Ctla4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ctla4
Entrez Gene ID	63835
Full Name	cytotoxic T-lymphocyte-associated protein 4
Synonyms	CTLA-4,Cd152,sCTLA4
General protein information	Preferred Names cytotoxic T-lymphocyte-associated protein 4 Names cytotoxic T-lymphocyte protein 4 CD152 antigen soluble form transmembrane form
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q32
Summary	immunoregulatory receptor expressed on the surface of T and B lymphocytes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245036.3	XP_006245098.1	cytotoxic T-lymphocyte protein 4 isoform X1
NM_031674.1	NP_113862.1	cytotoxic T-lymphocyte protein 4 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ctla4	NP_113862.1
Pan troglodytes (chimpanzee)	CTLA4	XP_526000.1
Mus musculus (house mouse)	Ctla4	NP_033973.2
Xenopus tropicalis (tropical clawed frog)	ctla4	XP_002936576.1
Bos taurus (cattle)	CTLA4	NP_776722.1
Homo sapiens (human)	CTLA4	NP_005205.2
	CTLA4	NP_001038204.1
Canis lupus familiaris (dog)	CTLA4	NP_001003106.1
Gallus gallus (chicken)	CTLA4	NP_001035180.1

Gene Ontology
Bibliography

Related articles in PubMed

Antibodies Against Immune Checkpoint Molecules Restore?Functions of Tumor-Infiltrating T Cells in Hepatocellular?Carcinomas.
Zhou G, Sprengers D, Boor PPC, Doukas M, Schutz H, Mancham S, Pedroza-Gonzalez A, Polak WG, de Jonge J, Gaspersz M, Dong H, Thielemans K, Pan Q, IJzermans JNM, Bruno MJ, Kwekkeboom J
Gastroenterology153(4)1107-1119.e10(2017 Oct)

Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab.
Ramagopal UA, Liu W, Garrett-Thomson SC, Bonanno JB, Yan Q, Srinivasan M, Wong SC, Bell A, Mankikar S, Rangan VS, Deshpande S, Korman AJ, Almo SC
Proceedings of the National Academy of Sciences of the United States of America114(21)E4223-E4232(2017 May)

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.
Schubert D, Bode C, Kenefeck R, Hou TZ, Wing JB, Kennedy A, Bulashevska A, Petersen BS, Sch?ffer AA, Gr?ning BA, Unger S, Frede N, Baumann U, Witte T, Schmidt RE, Dueckers G, Niehues T, Seneviratne S, Kanariou M, Speckmann C, Ehl S, Rensing-Ehl A, Warnatz K, Rakhmanov M, Thimme R, Hasselblatt P, Emmerich F, Cathomen T, Backofen R, Fisch P, Seidl M, May A, Schmitt-Graeff A, Ikemizu S, Salzer U, Franke A, Sakaguchi S, Walker LSK, Sansom DM, Grimbacher B
Nature medicine20(12)1410-1416(2014 Dec)

A DNA vaccine against cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) prevents tumor growth.
Lan KH, Liu YC, Shih YS, Tsaid CL, Yen SH, Lan KL
Biochemical and biophysical research communications440(2)222-8(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest the potential therapeutic applications for suppression of rheumatoid arthritis (RA) by local joint delivery of CTLA4-FasL.
Title: Intraarticular gene delivery of CTLA4-FasL suppresses experimental arthritis.

observations indicate that CTLA4-FasL protein represents a significantly suppressive effect on inflammatory fibroblast-like synoviocytes' proliferation
Title: CTLA4-FasL fusion product suppresses proliferation of fibroblast-like synoviocytes and progression of adjuvant-induced arthritis in rats.

The mixed lymphocyte reaction results indicate that ex vivo transfer of AdCTLA4Ig induces antigen-specific unresponsiveness. AdCTLA4Ig perfusion did not change the proportion of CD4+25+Foxp3+ T cells in recipients' spleens.
Title: Ex vivo transfer of adenovirus-mediated CTLA4Ig gene combined with a short course of rapamycin therapy prolongs free flap allograft survival.

CTLA-4 plays an important suppressive role in T-cell priming and protective immunity in a prime-challenge model of acute bacterial infection.
Title: Cytotoxic T-lymphocyte antigen 4 blockade augments the T-cell response primed by attenuated Listeria monocytogenes resulting in more rapid clearance of virulent bacterial challenge.

Administration of ethyl pyruvate to inhibit HMGB1 or anti-RAGE antibody could significantly decrease expression levels of CTLA-4, Foxp3 on Tregs, and IL-10 production after burns
Title: The effect of high-mobility group box 1 protein on activity of regulatory T cells after thermal injury in rats.

The following Ctla4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ctla4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08441	NM_031674.1 Latest version!	Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa32265	XM_006245036.3 Latest version!	Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ctla4 ( NM_031674.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113862.1 Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), mRNA.

Price & Availability↑

CloneID	ORa08441
Clone ID Related Accession (Same CDS sequence)	NM_031674.1
Accession Version	NM_031674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Rattus norvegicus(Norway rat)
Product	cytotoxic T-lymphocyte protein 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U37121.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U37121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTGTC TTGGACTCCA GAGGTACAAA ACTCACCTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGGAG TCCTGCTTTC TCTTCTCTTC ATCCCAATCT TCTCTGAAGC CATACAAGTG 
ACCCAACCTT CAGTGGTGTT GGCCAGCAGC CACGGTGTCG CCAGCTTTCC ATGTGAATAT 
GCATCTTCAC ACAACACTGA TGAGGTCCGG GTGACGGTGC TGCGGCAGAC AAATGACCAA 
GTGACAGAGG TCTGTGCCAC GACATTCACA GTGAAGAACA CGTTGGGCTT CCTAGATGAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGG 
GCTGCTGACA CCGGACTGTA CTTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC CCAGATTTAT GTCATCGATC CAGAACCATG CCCAGATTCA 
GACTTCCTCC TTTGGATCCT TGCTGCAGTT AGTTCGGGGT TGTTTTTCTA CAGTTTCCTG 
GTCACCGCTG TTTCTTTGAA CAGGACGCTA AAGAAAAGAA GTCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAACC TTATTTTATT 
CCAATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113862.1
CDS	1..672
Translation	MACLGLQRYKTHLQLPSRTWPFGVLLSLLFIPIFSEAIQVTQPSVVLASSHGVASFPCEYASSHNTDEVRVTVLRQTNDQVTEVCATTFTVKNTLGFLDDPFCSGTFNESRVNLTIQGLRAADTGLYFCKVELMYPPPYFVGMGNGTQIYVIDPEPCPDSDFLLWILAAVSSGLFFYSFLVTAVSLNRTLKKRSPLTTGVYVKMPPTEPECEKQFQPYFIPIN

Target ORF information:

RefSeq Version	NM_031674.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031674.1

ORF Insert Sequence:

ATGGCTTGTC TTGGACTCCA GAGGTACAAA ACTCACCTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGGAG TCCTGCTTTC TCTTCTCTTC ATCCCAATCT TCTCTGAAGC CATACAAGTG 
ACCCAACCTT CAGTGGTGTT GGCCAGCAGC CACGGTGTCG CCAGCTTTCC ATGTGAATAT 
GCATCTTCAC ACAACACTGA TGAGGTCCGG GTGACGGTGC TGCGGCAGAC AAATGACCAA 
GTGACAGAGG TCTGTGCCAC GACATTCACA GTGAAGAACA CGTTGGGCTT CCTAGATGAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGG 
GCTGCTGACA CCGGACTGTA CTTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAACGGGAC CCAGATTTAT GTCATCGATC CAGAACCATG CCCAGATTCA 
GACTTCCTCC TTTGGATCCT TGCTGCAGTT AGTTCGGGGT TGTTTTTCTA CAGTTTCCTG 
GTCACCGCTG TTTCTTTGAA CAGGACGCTA AAGAAAAGAA GTCCTCTTAC AACAGGGGTC 
TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAACC TTATTTTATT 
CCAATCAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ctla4 ( XM_006245036.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245098.1 Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32265
Clone ID Related Accession (Same CDS sequence)	XM_006245036.3
Accession Version	XM_006245036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytotoxic T-lymphocyte protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245036.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGTC TTGGAGTCCA GAGGTACAAA ACTCACCTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGGAG TCCTGCTTTC TCTTCTCTTC ATCCCAATCT TCTCTGAAGC CATACAAGTG 
ACCCAACCTT CAGTGGTGTT GGCCAGCAGC CACGGTGTCG CCAGCTTTCC ATGTGAATAT 
GCATCTTCAC ACAACACTGA TGAGGTCCGG GTGACGGTGC TGCGGCAGAC AAATGACCAA 
GTGACAGAGG TCTGTGCCAC GACATTCACA GTGAAGAACA CGTTGGGCTT CCTAGATGAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGG 
GCTGCTGACA CCGGACTGTA CTTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAATGGGAC CCAGATTTAT GTCATCGCTA AAGAAAAGAA GTCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245098.1
CDS	119..643
Translation	MARLGVQRYKTHLQLPSRTWPFGVLLSLLFIPIFSEAIQVTQPSVVLASSHGVASFPCEYASSHNTDEVRVTVLRQTNDQVTEVCATTFTVKNTLGFLDDPFCSGTFNESRVNLTIQGLRAADTGLYFCKVELMYPPPYFVGMGNGTQIYVIAKEKKSSYNRGLCENAPNRARM

Target ORF information:

RefSeq Version	XM_006245036.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytotoxic T-lymphocyte-associated protein 4 (Ctla4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245036.3

ORF Insert Sequence:

ATGGCTCGTC TTGGAGTCCA GAGGTACAAA ACTCACCTGC AGCTGCCTTC TAGGACTTGG 
CCTTTTGGAG TCCTGCTTTC TCTTCTCTTC ATCCCAATCT TCTCTGAAGC CATACAAGTG 
ACCCAACCTT CAGTGGTGTT GGCCAGCAGC CACGGTGTCG CCAGCTTTCC ATGTGAATAT 
GCATCTTCAC ACAACACTGA TGAGGTCCGG GTGACGGTGC TGCGGCAGAC AAATGACCAA 
GTGACAGAGG TCTGTGCCAC GACATTCACA GTGAAGAACA CGTTGGGCTT CCTAGATGAC 
CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGG 
GCTGCTGACA CCGGACTGTA CTTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT 
GTGGGCATGG GCAATGGGAC CCAGATTTAT GTCATCGCTA AAGAAAAGAA GTCCTCTTAC 
AACAGGGGTC TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antibodies Against Immune Checkpoint Molecules Restore?Functions of Tumor-Infiltrating T Cells in Hepatocellular?Carcinomas.
Gastroenterology153(4)1107-1119.e10(2017 Oct)
Zhou G,Sprengers D,Boor PPC,Doukas M,Schutz H,Mancham S,Pedroza-Gonzalez A,Polak WG,de Jonge J,Gaspersz M,Dong H,Thielemans K,Pan Q,IJzermans JNM,Bruno MJ,Kwekkeboom J

Structural basis for cancer immunotherapy by the first-in-class checkpoint inhibitor ipilimumab.
Proceedings of the National Academy of Sciences of the United States of America114(21)E4223-E4232(2017 May)
Ramagopal UA,Liu W,Garrett-Thomson SC,Bonanno JB,Yan Q,Srinivasan M,Wong SC,Bell A,Mankikar S,Rangan VS,Deshpande S,Korman AJ,Almo SC

Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.
Nature medicine20(12)1410-1416(2014 Dec)
Schubert D,Bode C,Kenefeck R,Hou TZ,Wing JB,Kennedy A,Bulashevska A,Petersen BS,Sch?ffer AA,Gr?ning BA,Unger S,Frede N,Baumann U,Witte T,Schmidt RE,Dueckers G,Niehues T,Seneviratne S,Kanariou M,Speckmann C,Ehl S,Rensing-Ehl A,Warnatz K,Rakhmanov M,Thimme R,Hasselblatt P,Emmerich F,Cathomen T,Backofen R,Fisch P,Seidl M,May A,Schmitt-Graeff A,Ikemizu S,Salzer U,Franke A,Sakaguchi S,Walker LSK,Sansom DM,Grimbacher B

A DNA vaccine against cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) prevents tumor growth.
Biochemical and biophysical research communications440(2)222-8(2013 Oct)
Lan KH,Liu YC,Shih YS,Tsaid CL,Yen SH,Lan KL

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Ctla4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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