Capn10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn10
Entrez Gene ID	63834
Full Name	calpain 10
General protein information	Preferred Names calpain 10 Names calpain-10 CANP 10 calcium-activated neutral proteinase 10
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q36
Summary	large subunit of calpain, a calcium-dependent cysteine protease; human homolog has been associated with type II diabetes mellitus [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031673.2	NP_113861.1	calpain-10

Pathways from BioSystems

Homologies

	CAPN10	XP_001107045.2
Rattus norvegicus (Norway rat)	Capn10	NP_113861.1
Danio rerio (zebrafish)	capn10	XP_698873.4
Homo sapiens (human)	CAPN10	NP_075571.1
Xenopus tropicalis (tropical clawed frog)	capn10	XP_004914447.1
Pan troglodytes (chimpanzee)	CAPN10	XP_003309611.1
Bos taurus (cattle)	LOC789674	XP_002686664.1
Canis lupus familiaris (dog)	CAPN10	XP_848308.1
Mus musculus (house mouse)	Capn10	NP_035926.2
Gallus gallus (chicken)	CAPN10	XP_004943444.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of calpain 10 causes mitochondrial dysfunction during chronic hyperglycemia.
Smith MA, Covington MD, Schnellmann RG
Archives of biochemistry and biophysics523(2)161-8(2012 Jul)

Chronic high glucose downregulates mitochondrial calpain 10 and contributes to renal cell death and diabetes-induced renal injury.
Covington MD, Schnellmann RG
Kidney international81(4)391-400(2012 Feb)

Association of diabetes susceptibility gene calpain-10 with pancreatic cancer among smokers.
Fong PY, Fesinmeyer MD, White E, Farin FM, Srinouanprachanh S, Afsharinejad Z, Mandelson MT, Brentnall TA, Barnett MJ, Goodman GE, Austin MA
Journal of gastrointestinal cancer41(3)203-8(2010 Sep)

Association of CAPN10 gene with insulin sensitivity, glucose tolerance and renal function in essential hypertensive patients.
Zhou X, Wang Y, Zhang Y, Gao P, Zhu D
Clinica chimica acta; international journal of clinical chemistry411(15-16)1126-31(2010 Aug)

Calpain-10 expression is elevated in pancreatic islets from patients with type 2 diabetes.
Ling C, Groop L, Guerra SD, Lupi R
PloS one4(8)e6558(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

diabetes-induced renal mitochondrial dysfunction and renal injury may directly result from the loss of renal calpain 10
Title: Loss of calpain 10 causes mitochondrial dysfunction during chronic hyperglycemia.

Calpain 10 levels decreased in the kidney, but not in the liver, with age, and this decrease was attenuated with caloric restriction.
Title: Calpain 10 is required for cell viability and is decreased in the aging kidney.

These results provide evidence that several previously postulated splice variants of calpain 10 are localized to the mitochondria in kidneys of rabbits, rats, and mice.
Title: Mitochondrial calpain 10 activity and expression in the kidney of multiple species.

Finally, we identified a 54 kDa isoform as the major CAPN10 isoform that associates with the actin cytoskeleton.
Title: Calpain facilitates actin reorganization during glucose-stimulated insulin secretion.

Data show that calpain 10 is a mitochondrially targeted and is a mediator of mitochondrial dysfunction through the cleavage of Complex I subunits and activation of the mitochondrial permeability transition.
Title: Calpain 10: a mitochondrial calpain and its role in calcium-induced mitochondrial dysfunction.

The following Capn10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08439	NM_031673.2 Latest version!	Rattus norvegicus calpain 10 (Capn10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn10 ( NM_031673.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113861.1 Rattus norvegicus calpain 10 (Capn10), mRNA.

Price & Availability↑

CloneID	ORa08439
Clone ID Related Accession (Same CDS sequence)	NM_031673.2
Accession Version	NM_031673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	calpain-10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085725.1 and AF227909.3. On Oct 11, 2005 this sequence version replaced NM_031673.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGCGG TCCGGGCCGA GACGCGGGCG CGGGAGCTCT TCCGGGACGC CGCATTCCCC 
GCCTCGGACT CCTCGCTCTT TTACAACTTG TCCACGCCTC TGGCCCAGTT TCGGGAGGAC 
ATCACTTGGA GACGGCCCCA GGACATTTGT GCCACACCTC AACTGTTTCC AGATAACCCA 
TGGGAAGGAC AGGTGAAGCA AGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGTGCC 
GCCCTTCAGA AGAGTCGACA CCTCCTGGAC CAGGTCTTCC CTCCAGGACA GCCAGGCTGG 
TCTGACCAGG AATACCAAGG CTTCTTCACC TGTCGGATTT GGCAGTTTGG ACACTGGGAG 
GAAGTGACCA TAGATGACCG TCTGCCTTGT CTTGCCGGGA GACTCTGCTT TTCTCGATGC 
CAGAGAGAGG ATGTGTTCTG GCTTCCCTTA CTGGAAAAGG CCTACGCTAA GGTTCATGGA 
TCCTATGAGC ACCTGTGGGC AGGGCAGGTG GCAGATGCCC TGGTGGATCT CACTGGCAGC 
CTGGCAGAGA GGTGGAGCCT GAAGGACATA AGGAAAGCCA GCGGCCAGCA GGACAGACCC 
AGTGGTGGAG AGCACAGAGC CTGTCAGCAG CTACTCCGTC TCAAGGACCA GTGTCTGCTC 
AGCTGCTCTG TGCTGAGCCC GAGAGCAGGT GCTAGGGAGC TCGGGGAGTT CCATGCCTTC 
ATTATCTCAG ATCTGCAGGA GCTCAGGAGT CAGACTGGTC AGGGTATCCT CCTGCTGAGG 
ATTCACAACC CTTGGGGCCG GCGTTGTTGG CAGGGCCTCT GGAGAGAAGG CGGTGAAGGG 
TGGAACCAGG TAGAGCCAGC TAAGGAGTCT GAGCTGCTGG CACAACTCCA GGAAGGAGAG 
TTCTGGGTAG AGGAAGAGGA GTTCCTCAGG GAGTTTGATG AGGTCACCAT CGGCTACCCA 
GTCACAGAGG CCGGCCACCT GCAAAGTCTC TACACAGAGA AGGTGCTGTG CCATACGCGG 
GCACTGCCTG GTGCCTGGGT GACAGGGCAA TCAGCTGGAG GCTGCCGGAA CAACAGTTGC 
TTTCCCTGCA ACCCCAAGTT CTGGTTACGG CTCTTGGAAC CCAGTGAGGT GTGTGTGGCT 
GTCCTTCAGA GACCCCGGAG GCGCTTAGTG GGCCAGACCC GGGCACTGGC GGGCGCCAGC 
CCTGCACCAG TGAACCTCCC AGGCAAAGAC TACCAGGCTG TGGGCCTGCA CATCTGGAAG 
GTAGAGAAAC GGAAGATCAG CCTGCCCAGA GTCCTGTCTG CACCCCCGGT GGCTGGCACT 
GCATGCCATG CGTATGATCG TGAGATCCAC TTGCGTTGTG AGCTCTCACC AGGCTACTAC 
CTGGCTGTCC CTAGCACCTT TTTGAAGGAT GTGCCAGGGC AGTTCCTGCT CCGAGTCTTC 
TCCACCGGGA AAATCTCCCT CAGTGCCGTC AGGCTGGCCA CCAAGGGTGC ATCACCTGGA 
GCAGCCCTGC CTGCAGGCGA GTGGGAGACT GTGCAGTTGC AGGGCAGCTG GAGAGCTGGC 
CAGACAGCCG GGGGCAGTAG AAACTTTGCC TCTTACCCCT GCAATCCCTG CCTCCCCTTC 
TCTGTTCCTG AGGGTGCTGG TCCCCGCTAC ATCCGTATCA CCCTACAGCA ACACTGCCGG 
CTCAGTGACA GCCAGCTCCA CCCCATTGGT TTCCATGTCT TTCAGGTTCC AGCAGACGGT 
GAAAAACAGG ATGCGTGTTC CCTGCTGCTC CAGGAGCCAC TGCTAAGCTG TGTACCACAT 
TGCTATGCCC AGGAAGTAAG CCGCCTCTGC CTCCTTTCTG CGGGGAACTA CAGGATTGTG 
CCCTCCACCT ACCTGCCAGA TACAGAGGGT ACCTTCACAG TGACCATAGC AACCAGAATA 
GACAGACAGT CCATTCACAG CCAGGAGATG CTGGGCCAGC TACTCCAGGA GGTCTCCTTC 
ATGGCAGTGA TGAAAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113861.1
CDS	124..2124
Translation	MRAVRAETRARELFRDAAFPASDSSLFYNLSTPLAQFREDITWRRPQDICATPQLFPDNPWEGQVKQGLLGDCWFLCACAALQKSRHLLDQVFPPGQPGWSDQEYQGFFTCRIWQFGHWEEVTIDDRLPCLAGRLCFSRCQREDVFWLPLLEKAYAKVHGSYEHLWAGQVADALVDLTGSLAERWSLKDIRKASGQQDRPSGGEHRACQQLLRLKDQCLLSCSVLSPRAGARELGEFHAFIISDLQELRSQTGQGILLLRIHNPWGRRCWQGLWREGGEGWNQVEPAKESELLAQLQEGEFWVEEEEFLREFDEVTIGYPVTEAGHLQSLYTEKVLCHTRALPGAWVTGQSAGGCRNNSCFPCNPKFWLRLLEPSEVCVAVLQRPRRRLVGQTRALAGASPAPVNLPGKDYQAVGLHIWKVEKRKISLPRVLSAPPVAGTACHAYDREIHLRCELSPGYYLAVPSTFLKDVPGQFLLRVFSTGKISLSAVRLATKGASPGAALPAGEWETVQLQGSWRAGQTAGGSRNFASYPCNPCLPFSVPEGAGPRYIRITLQQHCRLSDSQLHPIGFHVFQVPADGEKQDACSLLLQEPLLSCVPHCYAQEVSRLCLLSAGNYRIVPSTYLPDTEGTFTVTIATRIDRQSIHSQEMLGQLLQEVSFMAVMKA

Target ORF information:

RefSeq Version	NM_031673.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 10 (Capn10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031673.2

ORF Insert Sequence:

ATGCGGGCGG TCCGGGCCGA GACGCGGGCG CGGGAGCTCT TCCGGGACGC CGCATTCCCC 
GCCTCGGACT CCTCGCTCTT TTACAACTTG TCCACGCCTC TGGCCCAGTT TCGGGAGGAC 
ATCACTTGGA GACGGCCCCA GGACATTTGT GCCACACCTC AACTGTTTCC AGATAACCCA 
TGGGAAGGAC AGGTGAAGCA AGGGCTGCTG GGGGATTGCT GGTTCCTGTG TGCCTGTGCC 
GCCCTTCAGA AGAGTCGACA CCTCCTGGAC CAGGTCTTCC CTCCAGGACA GCCAGGCTGG 
TCTGACCAGG AATACCAAGG CTTCTTCACC TGTCGGATTT GGCAGTTTGG ACACTGGGAG 
GAAGTGACCA TAGATGACCG TCTGCCTTGT CTTGCCGGGA GACTCTGCTT TTCTCGATGC 
CAGAGAGAGG ATGTGTTCTG GCTTCCCTTA CTGGAAAAGG CCTACGCTAA GGTTCATGGA 
TCCTATGAGC ACCTGTGGGC AGGGCAGGTG GCAGATGCCC TGGTGGATCT CACTGGCAGC 
CTGGCAGAGA GGTGGAGCCT GAAGGACATA AGGAAAGCCA GCGGCCAGCA GGACAGACCC 
AGTGGTGGAG AGCACAGAGC CTGTCAGCAG CTACTCCGTC TCAAGGACCA GTGTCTGCTC 
AGCTGCTCTG TGCTGAGCCC GAGAGCAGGT GCTAGGGAGC TCGGGGAGTT CCATGCCTTC 
ATTATCTCAG ATCTGCAGGA GCTCAGGAGT CAGACTGGTC AGGGTATCCT CCTGCTGAGG 
ATTCACAACC CTTGGGGCCG GCGTTGTTGG CAGGGCCTCT GGAGAGAAGG CGGTGAAGGG 
TGGAACCAGG TAGAGCCAGC TAAGGAGTCT GAGCTGCTGG CACAACTCCA GGAAGGAGAG 
TTCTGGGTAG AGGAAGAGGA GTTCCTCAGG GAGTTTGATG AGGTCACCAT CGGCTACCCA 
GTCACAGAGG CCGGCCACCT GCAAAGTCTC TACACAGAGA AGGTGCTGTG CCATACGCGG 
GCACTGCCTG GTGCCTGGGT GACAGGGCAA TCAGCTGGAG GCTGCCGGAA CAACAGTTGC 
TTTCCCTGCA ACCCCAAGTT CTGGTTACGG CTCTTGGAAC CCAGTGAGGT GTGTGTGGCT 
GTCCTTCAGA GACCCCGGAG GCGCTTAGTG GGCCAGACCC GGGCACTGGC GGGCGCCAGC 
CCTGCACCAG TGAACCTCCC AGGCAAAGAC TACCAGGCTG TGGGCCTGCA CATCTGGAAG 
GTAGAGAAAC GGAAGATCAG CCTGCCCAGA GTCCTGTCTG CACCCCCGGT GGCTGGCACT 
GCATGCCATG CGTATGATCG TGAGATCCAC TTGCGTTGTG AGCTCTCACC AGGCTACTAC 
CTGGCTGTCC CTAGCACCTT TTTGAAGGAT GTGCCAGGGC AGTTCCTGCT CCGAGTCTTC 
TCCACCGGGA AAATCTCCCT CAGTGCCGTC AGGCTGGCCA CCAAGGGTGC ATCACCTGGA 
GCAGCCCTGC CTGCAGGCGA GTGGGAGACT GTGCAGTTGC AGGGCAGCTG GAGAGCTGGC 
CAGACAGCCG GGGGCAGTAG AAACTTTGCC TCTTACCCCT GCAATCCCTG CCTCCCCTTC 
TCTGTTCCTG AGGGTGCTGG TCCCCGCTAC ATCCGTATCA CCCTACAGCA ACACTGCCGG 
CTCAGTGACA GCCAGCTCCA CCCCATTGGT TTCCATGTCT TTCAGGTTCC AGCAGACGGT 
GAAAAACAGG ATGCGTGTTC CCTGCTGCTC CAGGAGCCAC TGCTAAGCTG TGTACCACAT 
TGCTATGCCC AGGAAGTAAG CCGCCTCTGC CTCCTTTCTG CGGGGAACTA CAGGATTGTG 
CCCTCCACCT ACCTGCCAGA TACAGAGGGT ACCTTCACAG TGACCATAGC AACCAGAATA 
GACAGACAGT CCATTCACAG CCAGGAGATG CTGGGCCAGC TACTCCAGGA GGTCTCCTTC 
ATGGCAGTGA TGAAAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of calpain 10 causes mitochondrial dysfunction during chronic hyperglycemia.
Archives of biochemistry and biophysics523(2)161-8(2012 Jul)
Smith MA,Covington MD,Schnellmann RG

Chronic high glucose downregulates mitochondrial calpain 10 and contributes to renal cell death and diabetes-induced renal injury.
Kidney international81(4)391-400(2012 Feb)
Covington MD,Schnellmann RG

Association of diabetes susceptibility gene calpain-10 with pancreatic cancer among smokers.
Journal of gastrointestinal cancer41(3)203-8(2010 Sep)
Fong PY,Fesinmeyer MD,White E,Farin FM,Srinouanprachanh S,Afsharinejad Z,Mandelson MT,Brentnall TA,Barnett MJ,Goodman GE,Austin MA

Association of CAPN10 gene with insulin sensitivity, glucose tolerance and renal function in essential hypertensive patients.
Clinica chimica acta; international journal of clinical chemistry411(15-16)1126-31(2010 Aug)
Zhou X,Wang Y,Zhang Y,Gao P,Zhu D

Calpain-10 expression is elevated in pancreatic islets from patients with type 2 diabetes.
PloS one4(8)e6558(2009 Aug)
Ling C,Groop L,Guerra SD,Lupi R

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Capn10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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