Unc5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Unc5b
Entrez Gene ID	60630
Full Name	unc-5 netrin receptor B
Synonyms	Unc5h2
General protein information	Preferred Names unc-5 netrin receptor B Names netrin receptor UNC5B protein unc-5 homolog 2 protein unc-5 homolog B transmembrane receptor Unc5H2 unc-5 homolog 2 unc-5 homolog B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	a netrin receptor involved in neuronal migration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256423.3	XP_006256485.1	netrin receptor UNC5B isoform X1
NM_022207.1	NP_071543.1	netrin receptor UNC5B precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	UNC5B	NP_734465.2
	UNC5B	XP_001106162.2
Gallus gallus (chicken)	UNC5B	NP_001026396.2
Caenorhabditis elegans (roundworm)	unc-5	NP_500823.1
Pan troglodytes (chimpanzee)	UNC5B	XP_001135844.2
Mus musculus (house mouse)	Unc5b	NP_084046.2
Rattus norvegicus (Norway rat)	Unc5b	NP_071543.1
Danio rerio (zebrafish)	unc5b	NP_001104619.1
Xenopus tropicalis (tropical clawed frog)	unc5b	XP_004916000.1
Canis lupus familiaris (dog)	UNC5B	XP_850428.1
Bos taurus (cattle)	UNC5B	NP_001092499.1

Gene Ontology
Bibliography

Related articles in PubMed

Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B.
Hayano Y, Takasu K, Koyama Y, Yamada M, Ogawa K, Minami K, Asaki T, Kitada K, Kuwabara S, Yamashita T
The Journal of experimental medicine213(13)2949-2966(2016 Dec)

Netrin-1 induces the migration of Schwann cells via p38 MAPK and PI3K-Akt signaling pathway mediated by the UNC5B receptor.
Lv J, Sun X, Ma J, Ma X, Zhang Y, Li F, Li Y, Zhao Z
Biochemical and biophysical research communications464(1)263-8(2015 Aug)

Effects of treadmill exercise on the expression of netrin-1 and its receptors in rat brain after cerebral ischemia.
Liu N, Huang H, Lin F, Chen A, Zhang Y, Chen R, Du H
Neuroscience194349-58(2011 Oct)

FLRT2 and FLRT3 act as repulsive guidance cues for Unc5-positive neurons.
Yamagishi S, Hampel F, Hata K, Del Toro D, Schwark M, Kvachnina E, Bastmeyer M, Yamashita T, Tarabykin V, Klein R, Egea J
The EMBO journal30(14)2920-33(2011 Jun)

The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase.
Guenebeaud C, Goldschneider D, Castets M, Guix C, Chazot G, Delloye-Bourgeois C, Eisenberg-Lerner A, Shohat G, Zhang M, Laudet V, Kimchi A, Bernet A, Mehlen P
Molecular cell40(6)863-76(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the Unc5B receptor and subsequent activation of the tyrosine phosphatase SHP2 mediated Netrin-4-induced pain signaling in the spinal cord.
Title: Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B.

Netrin-1 induces the migration of Schwann cells via p38 MAPK and PI3K-Akt signaling pathway mediated by the UNC5B receptor.
Title: Netrin-1 induces the migration of Schwann cells via p38 MAPK and PI3K-Akt signaling pathway mediated by the UNC5B receptor.

Treadmill exercise has beneficial effects via involvement of netrin-1 and its receptors DCC and Unc5B in neuronal and vascular activities in brain-ischemic rats.
Title: Effects of treadmill exercise on the expression of netrin-1 and its receptors in rat brain after cerebral ischemia.

Findings suggest that DCC:UNC5H ratios in DA neurons at critical periods may have important consequences for the organization and function of mesocorticolimbic DA systems.
Title: Peri-pubertal emergence of UNC-5 homologue expression by dopamine neurons in rodents.

These data suggest that netrin-1 could be an endogenous trophic factor for Schwann cells in the injured peripheral nerves.
Title: Netrin-1 induces proliferation of Schwann cells through Unc5b receptor.

The following Unc5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Unc5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39448	XM_006256423.3 Latest version!	Rattus norvegicus unc-5 netrin receptor B (Unc5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa05433	NM_022207.1 Latest version!	Rattus norvegicus unc-5 netrin receptor B (Unc5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Unc5b ( XM_006256423.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256485.1 Rattus norvegicus unc-5 netrin receptor B (Unc5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39448
Clone ID Related Accession (Same CDS sequence)	XM_006256423.3
Accession Version	XM_006256423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	netrin receptor UNC5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGCCC GGAGCGGGGC GCGGGGCGCG CTGCTGCTGC TGGCGCTGCT GCTCTGCTGG 
GATCCGACAC CGAGCTTAGC AGGCATTGAC TCTGGTGGCC AGGCACTCCC AGACTCCTTC 
CCATCAGCAC CCGCGGAGCA GCTGCCTCAC TTCCTGCTGG AACCAGAGGA TGCCTACATC 
GTAAAGAACA AGCCAGTGGA ATTGCACTGC CGAGCCTTCC CTGCCACACA GATCTACTTC 
AAGTGTAATG GCGAGTGGGT TAGCCAGAAA GGCCACGTCA CGCAGGAGAG CCTGGATGAG 
GCCACAGGCT TGCGAATACG AGAGGTGCAG ATAGAGGTGT CGCGGCAGCA GGTGGAGGAA 
CTCTTTGGGC TCGAGGACTA CTGGTGTCAG TGCGTGGCCT GGAGCTCTTC GGGAACCACC 
AAGAGTCGCC GAGCCTACAT CCGCATTGCC TACTTGCGCA AGAACTTTGA CCAGGAGCCT 
CTGGCGAAGG AGGTACCCTT GGATCATGAG GTCCTTCTGC AGTGCCGCCC ACCAGAGGGA 
GTGCCTGTGG CTGAGGTGGA ATGGCTCAAG AATGAAGATG TCATCGATCC CGCTCAGGAC 
ACTAACTTCC TGCTCACCAT TGACCACAAC CTCATCATCC GCCAGGCGCG CCTCTCAGAC 
ACAGCCAACT ACACCTGTGT GGCCAAGAAT ATTGTGGCCA AGCGCCGGAG CACGACGGCC 
ACAGTCATCG TCTATGTGAA CGGAGGTTGG TCCAGCTGGG CAGAATGGTC ACCCTGCTCT 
AACCGCTGCG GCCGAGGTTG GCAGAAACGT ACTAGGACCT GCACCAACCC AGCCCCACTC 
AATGGAGGTG CCTTCTGCGA GGGACAGGCT TTCCAGAAGA CGGCTTGCAC CACCGTGTGC 
CCAGTGGATG GAGCGTGGAC TGAGTGGAGC AAGTGGTCCG CCTGCAGCAC AGAGTGTGCG 
CACTGGCGCA GCCGCGAGTG CATGGCACCG CCGCCCCAGA ACGGAGGCCG CGACTGCAGC 
GGGACGCTAC TTGACTCCAA GAACTGCACC GATGGGCTGT GCGTGCTGAC CCTGGAGCCG 
TCGGGAGACG TGGCGCTGTA TGCGGGCCTC GTGGTGGCCG TCTTTGTGGT TCTGGCAGTT 
CTCATGGCTG TAGGAGTGAT CGTGTACCGG AGAAACTGCC GGGACTTCGA CACGGACATC 
ACTGACTCCT CTGCTGCCCT CACTGGTGGT TTCCACCCCG TCAACTTCAA GACTGCAAGG 
CCCAGCAACC CACAGCTCCT GCACCCATCC GCCCCTCCGG ACCTAACGGC CAGTGCTGGC 
ATCTACCGCG GACCTGTGTA TGCCCTGCAG GACTCCGCCG ACAAGATCCC TATGACTAAT 
TCACCCCTGC TGGATCCCTT GCCCAGCCTC AAGATCAAGG TCTATGACTC CAGCACCATC 
GGCTCTGGGG CTGGCCTGGC TGATGGAGCC GACCTGCTGG GTGTCTTACC ACCCGGTACA 
TACCCAGGCG ATTTCTCCCG GGACACCCAC TTCCTGCACC TGCGCAGCGC CAGCCTCGGT 
TCCCAGCACC TCCTGGGCCT CCCTCGAGAC CCCAGCAGCA GTGTCAGTGG CACCTTTGGT 
TGCCTGGGTG GGAGGCTGAC CATTCCCGGC ACAGGGGTCA GCCTGTTGGT ACCAAATGGA 
GCCATTCCCC AGGGCAAGTT CTATGACTTG TATCTACGTA TCAACAAGAC TGAAAGCACC 
CTCCCACTTT CAGAAGGTTC CCAGACAGTA TTGAGCCCCT CGGTGACCTG CGGGCCCACG 
GGCCTCCTCC TGTGCCGCCC TGTTGTCCTC ACTGTGCCCC ACTGTGCTGA AGTCATTGCC 
GGAGACTGGA TCTTCCAGCT CAAGACCCAG GCCCATCAGG GCCACTGGGA GGAGGTGGTG 
ACTTTGGATG AGGAGACTCT GAACACCCCC TGCTACTGCC AGCTAGAGGC TAAATCCTGC 
CACATCCTGT TGGACCAGCT GGGTACCTAC GTGTTCACGG GCGAGTCCTA CTCCCGCTCC 
GCAGTCAAGC GGCTCCAGCT AGCCATCTTC GCCCCAGCCC TCTGCACCTC CCTGGAGTAT 
AGTCTCAGGG TCTACTGTCT GGAGGACACT CCTGCAGCAC TGAAGGAGGT CCTAGAACTG 
GAGAGGACTC TGGGTGGCTA CTTGGTGGAG GAGCCCAAGC CTTTGCTCTT TAAGGACAGT 
TACCACAACC TACGCCTCTC CCTCCATGAC ATCCCCCATG CCCACTGGAG GAGCAAACTA 
CTGGCCAAGT ACCAGGAGAT TCCCTTCTAC CATGTGTGGA ACGGCAGCCA GAAAGCCCTG 
CACTGCACTT TCACCCTGGA GAGACATAGC CTAGCCTCCA CTGAGTTCAC CTGTAAGGTC 
TGCGTGCGGC AGGTAGAAGG GGAAGGCCAG ATTTTCCAGC TGCACACCAC GCTGGCTGAG 
ACGCCTGCTG GCTCCCTGGA TGCACTCTGC TCTGCCCCTG GCAATGCTGC CACCACACAG 
CTGGGACCCT ATGCCTTCAA GATACCACTG TCCATCCGCC AGAAGATCTG CAACAGCCTG 
GACGCCCCCA ACTCACGGGG CAATGACTGG CGGCTGTTGG CACAGAAGCT CTCCATGGAC 
CGGTACCTGA ACTACTTCGC CACCAAAGCT AGTCCCACAG GCGTGATCTT AGACCTCTGG 
GAAGCTCGGC AGCAGGATGA TGGGGACCTC AACAGCCTGG CCAGTGCCTT GGAGGAGATG 
GGCAAGAGTG AGATGCTGGT AGCCATGACC ACTGATGGCG ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256485.1
CDS	397..3204
Translation	MRARSGARGALLLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLTLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC

Target ORF information:

RefSeq Version	XM_006256423.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-5 netrin receptor B (Unc5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256423.3

ORF Insert Sequence:

ATGAGGGCCC GGAGCGGGGC GCGGGGCGCG CTGCTGCTGC TGGCGCTGCT GCTCTGCTGG 
GATCCGACAC CGAGCTTAGC AGGCATTGAC TCTGGTGGCC AGGCACTCCC AGACTCCTTC 
CCATCAGCAC CCGCGGAGCA GCTGCCTCAC TTCCTGCTGG AACCAGAGGA TGCCTACATC 
GTAAAGAACA AGCCAGTGGA ATTGCACTGC CGAGCCTTCC CTGCCACACA GATCTACTTC 
AAGTGTAATG GCGAGTGGGT TAGCCAGAAA GGCCACGTCA CGCAGGAGAG CCTGGATGAG 
GCCACAGGCT TGCGAATACG AGAGGTGCAG ATAGAGGTGT CGCGGCAGCA GGTGGAGGAA 
CTCTTTGGGC TCGAGGACTA CTGGTGTCAG TGCGTGGCCT GGAGCTCTTC GGGAACCACC 
AAGAGTCGCC GAGCCTACAT CCGCATTGCC TACTTGCGCA AGAACTTTGA CCAGGAGCCT 
CTGGCGAAGG AGGTACCCTT GGATCATGAG GTCCTTCTGC AGTGCCGCCC ACCAGAGGGA 
GTGCCTGTGG CTGAGGTGGA ATGGCTCAAG AATGAAGATG TCATCGATCC CGCTCAGGAC 
ACTAACTTCC TGCTCACCAT TGACCACAAC CTCATCATCC GCCAGGCGCG CCTCTCAGAC 
ACAGCCAACT ACACCTGTGT GGCCAAGAAT ATTGTGGCCA AGCGCCGGAG CACGACGGCC 
ACAGTCATCG TCTATGTGAA CGGAGGTTGG TCCAGCTGGG CAGAATGGTC ACCCTGCTCT 
AACCGCTGCG GCCGAGGTTG GCAGAAACGT ACTAGGACCT GCACCAACCC AGCCCCACTC 
AATGGAGGTG CCTTCTGCGA GGGACAGGCT TTCCAGAAGA CGGCTTGCAC CACCGTGTGC 
CCAGTGGATG GAGCGTGGAC TGAGTGGAGC AAGTGGTCCG CCTGCAGCAC AGAGTGTGCG 
CACTGGCGCA GCCGCGAGTG CATGGCACCG CCGCCCCAGA ACGGAGGCCG CGACTGCAGC 
GGGACGCTAC TTGACTCCAA GAACTGCACC GATGGGCTGT GCGTGCTGAC CCTGGAGCCG 
TCGGGAGACG TGGCGCTGTA TGCGGGCCTC GTGGTGGCCG TCTTTGTGGT TCTGGCAGTT 
CTCATGGCTG TAGGAGTGAT CGTGTACCGG AGAAACTGCC GGGACTTCGA CACGGACATC 
ACTGACTCCT CTGCTGCCCT CACTGGTGGT TTCCACCCCG TCAACTTCAA GACTGCAAGG 
CCCAGCAACC CACAGCTCCT GCACCCATCC GCCCCTCCGG ACCTAACGGC CAGTGCTGGC 
ATCTACCGCG GACCTGTGTA TGCCCTGCAG GACTCCGCCG ACAAGATCCC TATGACTAAT 
TCACCCCTGC TGGATCCCTT GCCCAGCCTC AAGATCAAGG TCTATGACTC CAGCACCATC 
GGCTCTGGGG CTGGCCTGGC TGATGGAGCC GACCTGCTGG GTGTCTTACC ACCCGGTACA 
TACCCAGGCG ATTTCTCCCG GGACACCCAC TTCCTGCACC TGCGCAGCGC CAGCCTCGGT 
TCCCAGCACC TCCTGGGCCT CCCTCGAGAC CCCAGCAGCA GTGTCAGTGG CACCTTTGGT 
TGCCTGGGTG GGAGGCTGAC CATTCCCGGC ACAGGGGTCA GCCTGTTGGT ACCAAATGGA 
GCCATTCCCC AGGGCAAGTT CTATGACTTG TATCTACGTA TCAACAAGAC TGAAAGCACC 
CTCCCACTTT CAGAAGGTTC CCAGACAGTA TTGAGCCCCT CGGTGACCTG CGGGCCCACG 
GGCCTCCTCC TGTGCCGCCC TGTTGTCCTC ACTGTGCCCC ACTGTGCTGA AGTCATTGCC 
GGAGACTGGA TCTTCCAGCT CAAGACCCAG GCCCATCAGG GCCACTGGGA GGAGGTGGTG 
ACTTTGGATG AGGAGACTCT GAACACCCCC TGCTACTGCC AGCTAGAGGC TAAATCCTGC 
CACATCCTGT TGGACCAGCT GGGTACCTAC GTGTTCACGG GCGAGTCCTA CTCCCGCTCC 
GCAGTCAAGC GGCTCCAGCT AGCCATCTTC GCCCCAGCCC TCTGCACCTC CCTGGAGTAT 
AGTCTCAGGG TCTACTGTCT GGAGGACACT CCTGCAGCAC TGAAGGAGGT CCTAGAACTG 
GAGAGGACTC TGGGTGGCTA CTTGGTGGAG GAGCCCAAGC CTTTGCTCTT TAAGGACAGT 
TACCACAACC TACGCCTCTC CCTCCATGAC ATCCCCCATG CCCACTGGAG GAGCAAACTA 
CTGGCCAAGT ACCAGGAGAT TCCCTTCTAC CATGTGTGGA ACGGCAGCCA GAAAGCCCTG 
CACTGCACTT TCACCCTGGA GAGACATAGC CTAGCCTCCA CTGAGTTCAC CTGTAAGGTC 
TGCGTGCGGC AGGTAGAAGG GGAAGGCCAG ATTTTCCAGC TGCACACCAC GCTGGCTGAG 
ACGCCTGCTG GCTCCCTGGA TGCACTCTGC TCTGCCCCTG GCAATGCTGC CACCACACAG 
CTGGGACCCT ATGCCTTCAA GATACCACTG TCCATCCGCC AGAAGATCTG CAACAGCCTG 
GACGCCCCCA ACTCACGGGG CAATGACTGG CGGCTGTTGG CACAGAAGCT CTCCATGGAC 
CGGTACCTGA ACTACTTCGC CACCAAAGCT AGTCCCACAG GCGTGATCTT AGACCTCTGG 
GAAGCTCGGC AGCAGGATGA TGGGGACCTC AACAGCCTGG CCAGTGCCTT GGAGGAGATG 
GGCAAGAGTG AGATGCTGGT AGCCATGACC ACTGATGGCG ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Unc5b ( NM_022207.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071543.1 Rattus norvegicus unc-5 netrin receptor B (Unc5b), mRNA.

Price & Availability↑

CloneID	ORa05433
Clone ID Related Accession (Same CDS sequence)	NM_022207.1
Accession Version	NM_022207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	netrin receptor UNC5B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U87306.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U87306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGGGCCC GGAGCGGGGC GCGGGGCGCG CTGCTGCTGG CGCTGCTGCT CTGCTGGGAT 
CCGACACCGA GCTTAGCAGG CATTGACTCT GGTGGCCAGG CACTCCCAGA CTCCTTCCCA 
TCAGCACCCG CGGAGCAGCT GCCTCACTTC CTGCTGGAAC CAGAGGATGC CTACATCGTA 
AAGAACAAGC CAGTGGAATT GCACTGCCGA GCCTTCCCTG CCACACAGAT CTACTTCAAG 
TGTAATGGCG AGTGGGTTAG CCAGAAAGGC CACGTCACGC AGGAGAGCCT GGATGAGGCC 
ACAGGCTTGC GAATACGAGA GGTGCAGATA GAGGTGTCGC GGCAGCAGGT GGAGGAACTT 
TTTGGGCTCG AGGACTACTG GTGTCAGTGC GTGGCCTGGA GCTCTTCGGG AACCACCAAG 
AGTCGCCGAG CCTACATCCG CATTGCCTAC TTGCGCAAGA ACTTTGACCA GGAGCCTCTG 
GCGAAGGAGG TACCCTTGGA TCATGAGGTC CTTCTGCAGT GCCGCCCACC AGAGGGAGTG 
CCTGTGGCTG AGGTGGAATG GCTCAAGAAT GAAGATGTCA TCGATCCCGC TCAGGACACT 
AACTTCCTGC TCACCATTGA CCACAACCTC ATCATCCGCC AGGCGCGCCT CTCAGACACA 
GCCAACTACA CCTGTGTGGC AAAGAATATT GTGGCCAAGC GCCGGAGCAC GACGGCCACA 
GTCATCGTCT ATGTGAACGG AGGTTGGTCC AGCTGGGCAG AATGGTCACC CTGCTCTAAC 
CGCTGCGGCC GAGGTTGGCA GAAACGTACT AGGACCTGCA CCAACCCAGC CCCACTCAAT 
GGAGGTGCCT TCTGCGAGGG ACAGGCTTGC CAGAAGACGG CTTGCACCAC CGTGTGCCCA 
GTGGATGGAG CGTGGACTGA GTGGAGCAAG TGGTCCGCCT GCAGCACAGA GTGTGCGCAC 
TGGCGCAGCC GCGAGTGCAT GGCACCGCCG CCCCAGAACG GAGGCCGTGA CTGCAGCGGG 
ACGCTACTTG ACTCCAAGAA CTGCACCGAT GGGCTGTGCG TGCTGAATCA GAGAACTCTA 
AACGACCCTA AAAGCCGCCC CCTGGAGCCG TCGGGAGACG TGGCGCTGTA TGCGGGCCTC 
GTGGTGGCCG TCTTTGTGGT TCTGGCAGTT CTCATGGCTG TAGGAGTGAT CGTGTACCGG 
AGAAACTGCC GGGACTTCGA CACGGACATC ACTGACTCCT CTGCTGCCCT CACTGGTGGT 
TTCCACCCCG TCAACTTCAA GACTGCAAGG CCCAGCAACC CACAGCTCCT GCACCCATCC 
GCCCCTCCGG ACCTAACGGC CAGTGCTGGC ATCTACCGCG GACCTGTGTA TGCCCTGCAG 
GACTCTGCCG ACAAGATCCC TATGACTAAT TCACCCCTTC TGGATCCCTT GCCCAGCCTC 
AAGATCAAGG TCTATGACTC CAGCACCATC GGCTCTGGGG CTGGCCTGGC TGATGGAGCC 
GACCTGCTGG GTGTCTTACC ACCCGGTACA TACCCAGGCG ATTTCTCCCG GGACACCCAC 
TTCCTGCACC TGCGCAGCGC CAGCCTTGGT TCCCAGCACC TCCTGGGCCT CCCTCGAGAC 
CCCAGCAGCA GTGTCAGTGG CACCTTTGGT TGCCTGGGTG GGAGGCTGAC CATTCCCGGC 
ACAGGGGTCA GCCTGTTGGT ACCAAATGGA GCCATTCCCC AGGGCAAGTT CTATGACTTG 
TATCTACGTA TCAACAAGAC TGAAAGCACC CTCCCACTTT CGGAAGGTTC CCAGACAGTA 
TTGAGCCCCT CGGTGACCTG CGGGCCCACG GGCCTCCTCC TGTGCCGCCC TGTTGTCCTC 
ACTGTGCCCC ACTGTGCTGA AGTCATTGCC GGAGACTGGA TCTTCCAGCT CAAGACCCAG 
GCCCATCAGG GCCACTGGGA GGAGGTGGTG ACTTTGGATG AGGAGACTCT GAACACCCCC 
TGCTACTGCC AGCTAGAGGC TAAATCCTGC CACATCCTGT TGGACCAGCT GGGTACCTAC 
GTGTTCACGG GCGAGTCCTA CTCCCGCTCC GCAGTCAAGC GGCTCCAGCT AGCCATCTTC 
GCCCCAGCCC TCTGCACCTC CCTGGAGTAT AGTCTCAGGG TCTACTGTCT GGAGGACACT 
CCTGCAGCAC TGAAGGAGGT CCTAGAGCTG GAGAGGACTC TGGGTGGCTA CTTGGTGGAG 
GAGCCCAAGA CTTTGCTCTT TAAGGACAGT TACCACAACC TACGNCTCTC CCTCCATGAC 
ATCCCCCATG CCCACTGGAG GAGCAAACTA CTGGCCAAGT ACCAGGAGAT TCCCTTCTAC 
CATGTGTGGA ACGGCAGCCA GAAAGCCCTG CACTGCACTT TCACCCTGGA GAGACATAGC 
CTAGCCTCCA CTGAGTTCAC CTGTAAGGTC TGCGTGCGGC AGGTAGAAGG GGAAGGCCAG 
ATTTTCCAGC TGCACACCAC GCTGGCTGAG ACGCCTGCTG GCTCCCTGGA TGCACTCTGC 
TCTGCCCCTG GCAATGCTGC CACCACACAG CTGGGACCCT ATGCCTTCAA GATACCACTG 
TCCATCCGCC AGAAGATCTG CAACAGCCTG GACGCCCCCA ACTCACGGGG CAATGACTGG 
CGGCTGTTGG CACAGAAGCT CTCCATGGAC CGGTACCTGA ACTACTTCGC CACCAAAGCT 
AGTCCCACAG GCGTGATCTT AGACCTCTGG GAAGCTCGGC AGCAGGATGA TGGGGACCTC 
AACAGCCTGG CCAGTGCCTT GGAGGAGATG GGCAAGAGTG AGATGCTGGT AGCCATGACC 
ACTGATGGCG ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071543.1
CDS	1..2838
Translation	MRARSGARGALLLALLLCWDPTPSLAGIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGDVALYAGLVVAVFVVLAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYDSSTIGSGAGLADGADLLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLTIPGTGVSLLVPNGAIPQGKFYDLYLRINKTESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFTGESYSRSAVKRLQLAIFAPALCTSLEYSLRVYCLEDTPAALKEVLELERTLGGYLVEEPKTLLFKDSYHNLnullLSLHDIPHAHWRSKLLAKYQEIPFYHVWNGSQKALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAATTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLNSLASALEEMGKSEMLVAMTTDGDC

Target ORF information:

RefSeq Version	NM_022207.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus unc-5 netrin receptor B (Unc5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022207.1

ORF Insert Sequence:

ATGAGGGCCC GGAGCGGGGC GCGGGGCGCG CTGCTGCTGG CGCTGCTGCT CTGCTGGGAT 
CCGACACCGA GCTTAGCAGG CATTGACTCT GGTGGCCAGG CACTCCCAGA CTCCTTCCCA 
TCAGCACCCG CGGAGCAGCT GCCTCACTTC CTGCTGGAAC CAGAGGATGC CTACATCGTA 
AAGAACAAGC CAGTGGAATT GCACTGCCGA GCCTTCCCTG CCACACAGAT CTACTTCAAG 
TGTAATGGCG AGTGGGTTAG CCAGAAAGGC CACGTCACGC AGGAGAGCCT GGATGAGGCC 
ACAGGCTTGC GAATACGAGA GGTGCAGATA GAGGTGTCGC GGCAGCAGGT GGAGGAACTT 
TTTGGGCTCG AGGACTACTG GTGTCAGTGC GTGGCCTGGA GCTCTTCGGG AACCACCAAG 
AGTCGCCGAG CCTACATCCG CATTGCCTAC TTGCGCAAGA ACTTTGACCA GGAGCCTCTG 
GCGAAGGAGG TACCCTTGGA TCATGAGGTC CTTCTGCAGT GCCGCCCACC AGAGGGAGTG 
CCTGTGGCTG AGGTGGAATG GCTCAAGAAT GAAGATGTCA TCGATCCCGC TCAGGACACT 
AACTTCCTGC TCACCATTGA CCACAACCTC ATCATCCGCC AGGCGCGCCT CTCAGACACA 
GCCAACTACA CCTGTGTGGC AAAGAATATT GTGGCCAAGC GCCGGAGCAC GACGGCCACA 
GTCATCGTCT ATGTGAACGG AGGTTGGTCC AGCTGGGCAG AATGGTCACC CTGCTCTAAC 
CGCTGCGGCC GAGGTTGGCA GAAACGTACT AGGACCTGCA CCAACCCAGC CCCACTCAAT 
GGAGGTGCCT TCTGCGAGGG ACAGGCTTGC CAGAAGACGG CTTGCACCAC CGTGTGCCCA 
GTGGATGGAG CGTGGACTGA GTGGAGCAAG TGGTCCGCCT GCAGCACAGA GTGTGCGCAC 
TGGCGCAGCC GCGAGTGCAT GGCACCGCCG CCCCAGAACG GAGGCCGTGA CTGCAGCGGG 
ACGCTACTTG ACTCCAAGAA CTGCACCGAT GGGCTGTGCG TGCTGAATCA GAGAACTCTA 
AACGACCCTA AAAGCCGCCC CCTGGAGCCG TCGGGAGACG TGGCGCTGTA TGCGGGCCTC 
GTGGTGGCCG TCTTTGTGGT TCTGGCAGTT CTCATGGCTG TAGGAGTGAT CGTGTACCGG 
AGAAACTGCC GGGACTTCGA CACGGACATC ACTGACTCCT CTGCTGCCCT CACTGGTGGT 
TTCCACCCCG TCAACTTCAA GACTGCAAGG CCCAGCAACC CACAGCTCCT GCACCCATCC 
GCCCCTCCGG ACCTAACGGC CAGTGCTGGC ATCTACCGCG GACCTGTGTA TGCCCTGCAG 
GACTCTGCCG ACAAGATCCC TATGACTAAT TCACCCCTTC TGGATCCCTT GCCCAGCCTC 
AAGATCAAGG TCTATGACTC CAGCACCATC GGCTCTGGGG CTGGCCTGGC TGATGGAGCC 
GACCTGCTGG GTGTCTTACC ACCCGGTACA TACCCAGGCG ATTTCTCCCG GGACACCCAC 
TTCCTGCACC TGCGCAGCGC CAGCCTTGGT TCCCAGCACC TCCTGGGCCT CCCTCGAGAC 
CCCAGCAGCA GTGTCAGTGG CACCTTTGGT TGCCTGGGTG GGAGGCTGAC CATTCCCGGC 
ACAGGGGTCA GCCTGTTGGT ACCAAATGGA GCCATTCCCC AGGGCAAGTT CTATGACTTG 
TATCTACGTA TCAACAAGAC TGAAAGCACC CTCCCACTTT CGGAAGGTTC CCAGACAGTA 
TTGAGCCCCT CGGTGACCTG CGGGCCCACG GGCCTCCTCC TGTGCCGCCC TGTTGTCCTC 
ACTGTGCCCC ACTGTGCTGA AGTCATTGCC GGAGACTGGA TCTTCCAGCT CAAGACCCAG 
GCCCATCAGG GCCACTGGGA GGAGGTGGTG ACTTTGGATG AGGAGACTCT GAACACCCCC 
TGCTACTGCC AGCTAGAGGC TAAATCCTGC CACATCCTGT TGGACCAGCT GGGTACCTAC 
GTGTTCACGG GCGAGTCCTA CTCCCGCTCC GCAGTCAAGC GGCTCCAGCT AGCCATCTTC 
GCCCCAGCCC TCTGCACCTC CCTGGAGTAT AGTCTCAGGG TCTACTGTCT GGAGGACACT 
CCTGCAGCAC TGAAGGAGGT CCTAGAGCTG GAGAGGACTC TGGGTGGCTA CTTGGTGGAG 
GAGCCCAAGA CTTTGCTCTT TAAGGACAGT TACCACAACC TACGNCTCTC CCTCCATGAC 
ATCCCCCATG CCCACTGGAG GAGCAAACTA CTGGCCAAGT ACCAGGAGAT TCCCTTCTAC 
CATGTGTGGA ACGGCAGCCA GAAAGCCCTG CACTGCACTT TCACCCTGGA GAGACATAGC 
CTAGCCTCCA CTGAGTTCAC CTGTAAGGTC TGCGTGCGGC AGGTAGAAGG GGAAGGCCAG 
ATTTTCCAGC TGCACACCAC GCTGGCTGAG ACGCCTGCTG GCTCCCTGGA TGCACTCTGC 
TCTGCCCCTG GCAATGCTGC CACCACACAG CTGGGACCCT ATGCCTTCAA GATACCACTG 
TCCATCCGCC AGAAGATCTG CAACAGCCTG GACGCCCCCA ACTCACGGGG CAATGACTGG 
CGGCTGTTGG CACAGAAGCT CTCCATGGAC CGGTACCTGA ACTACTTCGC CACCAAAGCT 
AGTCCCACAG GCGTGATCTT AGACCTCTGG GAAGCTCGGC AGCAGGATGA TGGGGACCTC 
AACAGCCTGG CCAGTGCCTT GGAGGAGATG GGCAAGAGTG AGATGCTGGT AGCCATGACC 
ACTGATGGCG ATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B.
The Journal of experimental medicine213(13)2949-2966(2016 Dec)
Hayano Y,Takasu K,Koyama Y,Yamada M,Ogawa K,Minami K,Asaki T,Kitada K,Kuwabara S,Yamashita T

Netrin-1 induces the migration of Schwann cells via p38 MAPK and PI3K-Akt signaling pathway mediated by the UNC5B receptor.
Biochemical and biophysical research communications464(1)263-8(2015 Aug)
Lv J,Sun X,Ma J,Ma X,Zhang Y,Li F,Li Y,Zhao Z

Effects of treadmill exercise on the expression of netrin-1 and its receptors in rat brain after cerebral ischemia.
Neuroscience194349-58(2011 Oct)
Liu N,Huang H,Lin F,Chen A,Zhang Y,Chen R,Du H

FLRT2 and FLRT3 act as repulsive guidance cues for Unc5-positive neurons.
The EMBO journal30(14)2920-33(2011 Jun)
Yamagishi S,Hampel F,Hata K,Del Toro D,Schwark M,Kvachnina E,Bastmeyer M,Yamashita T,Tarabykin V,Klein R,Egea J

The dependence receptor UNC5H2/B triggers apoptosis via PP2A-mediated dephosphorylation of DAP kinase.
Molecular cell40(6)863-76(2010 Dec)
Guenebeaud C,Goldschneider D,Castets M,Guix C,Chazot G,Delloye-Bourgeois C,Eisenberg-Lerner A,Shohat G,Zhang M,Laudet V,Kimchi A,Bernet A,Mehlen P

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Unc5b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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