Slc28a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc28a2
Entrez Gene ID	60423
Full Name	solute carrier family 28 member 2
General protein information	Preferred Names solute carrier family 28 member 2 Names sodium/nucleoside cotransporter 2 CNT 2 Na(+)/nucleoside cotransporter 2 SPNT concentrative nucleoside transporter 2 rCNT2 sodium-coupled nucleoside transporter 2 sodium/purine nucleoside cotransporter solute carrier family 28 (concentrative nucleoside transporter), member 2 solute carrier family 28 (sodium-coupled nucleoside transporter), member 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	regulates adenosine transport across the blood-brain barrierinto the brain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031664.1	NP_113852.1	sodium/nucleoside cotransporter 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC28A2	NP_004203.2
Pan troglodytes (chimpanzee)	SLC28A2	XP_510372.1
Gallus gallus (chicken)	SLC28A2	XP_004943667.1
Bos taurus (cattle)	SLC28A2	XP_002690952.2
Rattus norvegicus (Norway rat)	Slc28a2	NP_113852.1
	SLC28A2	XP_001111449.2
Xenopus tropicalis (tropical clawed frog)	slc28a2	XP_002934615.2
Mus musculus (house mouse)	Slc28a2	NP_766568.1
Danio rerio (zebrafish)	si:dkey-24l11.1	NP_001074966.1
Drosophila melanogaster (fruit fly)	CNT1	NP_610447.2

Gene Ontology
Bibliography

Related articles in PubMed

Localization of nucleoside transporters in rat epididymis.
Klein DM, Harding MC, Crowther MK, Cherrington NJ
Journal of biochemical and molecular toxicology31(8)(2017 Aug)

rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.
Pinilla-Macua I, Claudio-Montero A, Pastor-Anglada M
FEBS letters588(23)4382-9(2014 Nov)

Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2?in differentiated neuronal PC12 cells.
Medina-Pulido L, Molina-Arcas M, Justicia C, Soriano E, Burgaya F, Planas AM, Pastor-Anglada M
The Biochemical journal454(3)437-45(2013 Sep)

Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.
Pinilla-Macua I, Casado FJ, Pastor-Anglada M
The Biochemical journal442(3)517-25(2012 Mar)

Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.
Nishimura T, Chishu T, Tomi M, Nakamura R, Sato K, Kose N, Sai Y, Nakashima E
Drug metabolism and pharmacokinetics27(4)439-46(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The purpose of this study is to determine the localization of nucleoside transporters expressed within rat epididymis to demonstrate the potential of epididymal reabsorption. Using immunohistochemistry, we determined that equilibrative nucleoside transporter 1 (ENT1) is localized to the basolateral membrane of epithelial cells, ENT2 is expressed in the nucleus of the epithelium and CNT2 is expressed by basal cells.
Title: Localization of nucleoside transporters in rat epididymis.

rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane
Title: rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.

CNT2 is likely to modulate extracellular adenosine and cell energy balance in neuronal tissue.
Title: Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2?in differentiated neuronal PC12 cells.

the N-terminal tail of rCNT2 contains dual sorting signals. An acidic region is responsible for its proper stabilization at the plasma membrane, whereas the putative CK2 domain (Ser(46)) is implicated in the apical sorting of the transporter.
Title: Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.

Report induction of CNT2 in experimental diabetes mellitus.
Title: Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.

The following Slc28a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc28a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08416	NM_031664.1 Latest version!	Rattus norvegicus solute carrier family 28 member 2 (Slc28a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc28a2 ( NM_031664.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113852.1 Rattus norvegicus solute carrier family 28 member 2 (Slc28a2), mRNA.

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CloneID	ORa08416
Clone ID Related Accession (Same CDS sequence)	NM_031664.1
Accession Version	NM_031664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	sodium/nucleoside cotransporter 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U25055.1. On Jan 12, 2005 this sequence version replaced XM_345427.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U66723.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN02642802 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGT CAGAGGGAAG AAAGTCTGCT TCCCAGGACA CATCGGAGAA TGGCATGGAG 
AACCCAGGCC TGGAGCTCAT GGAAGTCGGA AACCTTGAGC AAGGCAAAAC ACTGGAGGAA 
GTGACACAGG GACATAGCCT GAAGGATGGC TTGGGGCATT CCTCTCTCTG GAGGAGGATT 
CTTCAGCCCT TCACCAAGGC GAGAAGTTTC TATCAAAGAC ATGCTGGTTT ATTCAAAAAG 
ATCCTGCTTG GTCTCTTGTG TTTAGCGTAT GCTGCCTACC TCCTGGCAGC TTGCATCCTG 
AACTTCCGGA GGGCACTGGC CTTGTTTGTC ATCACCTGCC TTGTCATCTT CATCCTGGCT 
TGCCACTTTC TGAAAAAATT CTTTGCTAAA AAGTCAATAA GATGCTTGAA GCCCTTGAAA 
AACACCCGCC TGAGACTTTG GCTTAAGAGG GTGTTCATGG GAGCCGCTGT TGTCGGCCTT 
ATTCTCTGGC TGGCTCTGGA CACAGCTCAA AGGCCAGAGC AGCTGATCTC CTTTGCGGGA 
ATCTGCATGT TCATCCTCAT CCTCTTTGCC TGCTCCAAAC ACCACAGTGC GGTGAGCTGG 
AGGACAGTGT TTTGGGGCCT GGGCCTTCAG TTCGTCTTTG GGATTTTGGT CATCAGAACT 
GAACCTGGAT TTAATGCATT TCAATGGCTG GGAGATCAGA TCCAGATTTT CCTGGCATAT 
ACTGTAGAAG GATCCAGTTT TGTTTTTGGT GACACACTGG TCCAAAGTGT TTTTGCTTTT 
CAGTCTCTCC CAATCATTAT TTTCTTCGGA TGTGTGATGT CTATTCTCTA CTACCTGGGC 
CTTGTGCAGT GGGTGATCCA GAAGATTGCC TGGTTCCTGC AAATCACCAT GGGTACCACA 
GCTGCAGAGA CCCTGGCCGT GGCAGGAAAC ATCTTTGTGG GAATGACAGA GGCACCTCTG 
CTCATCCGTC CCTACCTTGC AGACATGACC CTCTCTGAAA TCCATGCAGT GATGACTGGA 
GGCTTTGCTA CTATAGCAGG CACAGTGTTG GGAGCCTTCA TATCCTTTGG GATTGATGCA 
TCATCCTTGA TTTCTGCCTC AGTGATGGCT GCCCCTTGTG CACTTGCCTT GTCCAAACTG 
GTATATCCAG AAGTAGAAGA GTCCAAGTTC AAGAGCAAGG AGGGAGTGAA GCTGCCTCGA 
GGGGAAGAGA GGAATATTCT GGAAGCCGCG AGCAACGGAG CCACAGATGC CATAGCGCTT 
GTTGCTAATG TTGCAGCCAA CCTGATTGCC TTTCTGGCTG TATTGGCCTT CATCAATTCT 
ACCCTTTCCT GGCTGGGGGA AATGGTGGAT ATCCATGGAC TCACTTTCCA GGTCATCTGC 
TCCTATGTCC TAAGGCCAAT GGTTTTCATG ATGGGTGTAC AGTGGGCAGA CTGCCCATTG 
GTGGCTGAGA TAGTGGGAGT CAAATTCTTC ATAAATGAAT TTGTGGCCTA TCAACAACTG 
TCTCAGTACA AGAACAAACG TCTCTCTGGA GTCGAAGAGT GGATCAATGG CGAGAAACAG 
TGGATTTCTG TGAAGGCTGA AATCATTGCA ACATTTTCTC TCTGTGGATT TGCCAATCTT 
ACTTCCATAG GGATCACACT GGGAGGCTTG ACATCCATGG TTCCCCAACG GAAGAGTGAC 
TTGTGCAAGC TTGTGGTCAG GGCCCTCTTC ACAGGTGCCT GTGTTTCCTT CATCAGTGCC 
TGTATGGCAG GAATCCTCTA TGTTCCCAGA GGCGCTGAAA CTGACTGTGT CTCATTCTTA 
AACACAAATT TCACCAACAG AACCTATGAG ACATATGTGT GCTGCAGAGA GCTCTTCCAG 
AGTACTTTGC TGAATGGCAC CAACATGCCT TCTTTCTCAG GCCCCTGGCA AGACAAGGAG 
TCCAGCCTCA GGAATCTTGC TAAATGCTGT GACCTCTATA CCAGCACTGT GTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113852.1
CDS	272..2251
Translation	MAKSEGRKSASQDTSENGMENPGLELMEVGNLEQGKTLEEVTQGHSLKDGLGHSSLWRRILQPFTKARSFYQRHAGLFKKILLGLLCLAYAAYLLAACILNFRRALALFVITCLVIFILACHFLKKFFAKKSIRCLKPLKNTRLRLWLKRVFMGAAVVGLILWLALDTAQRPEQLISFAGICMFILILFACSKHHSAVSWRTVFWGLGLQFVFGILVIRTEPGFNAFQWLGDQIQIFLAYTVEGSSFVFGDTLVQSVFAFQSLPIIIFFGCVMSILYYLGLVQWVIQKIAWFLQITMGTTAAETLAVAGNIFVGMTEAPLLIRPYLADMTLSEIHAVMTGGFATIAGTVLGAFISFGIDASSLISASVMAAPCALALSKLVYPEVEESKFKSKEGVKLPRGEERNILEAASNGATDAIALVANVAANLIAFLAVLAFINSTLSWLGEMVDIHGLTFQVICSYVLRPMVFMMGVQWADCPLVAEIVGVKFFINEFVAYQQLSQYKNKRLSGVEEWINGEKQWISVKAEIIATFSLCGFANLTSIGITLGGLTSMVPQRKSDLCKLVVRALFTGACVSFISACMAGILYVPRGAETDCVSFLNTNFTNRTYETYVCCRELFQSTLLNGTNMPSFSGPWQDKESSLRNLAKCCDLYTSTVCA

Target ORF information:

RefSeq Version	NM_031664.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 28 member 2 (Slc28a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031664.1

ORF Insert Sequence:

ATGGCGAAGT CAGAGGGAAG AAAGTCTGCT TCCCAGGACA CATCGGAGAA TGGCATGGAG 
AACCCAGGCC TGGAGCTCAT GGAAGTCGGA AACCTTGAGC AAGGCAAAAC ACTGGAGGAA 
GTGACACAGG GACATAGCCT GAAGGATGGC TTGGGGCATT CCTCTCTCTG GAGGAGGATT 
CTTCAGCCCT TCACCAAGGC GAGAAGTTTC TATCAAAGAC ATGCTGGTTT ATTCAAAAAG 
ATCCTGCTTG GTCTCTTGTG TTTAGCGTAT GCTGCCTACC TCCTGGCAGC TTGCATCCTG 
AACTTCCGGA GGGCACTGGC CTTGTTTGTC ATCACCTGCC TTGTCATCTT CATCCTGGCT 
TGCCACTTTC TGAAAAAATT CTTTGCTAAA AAGTCAATAA GATGCTTGAA GCCCTTGAAA 
AACACCCGCC TGAGACTTTG GCTTAAGAGG GTGTTCATGG GAGCCGCTGT TGTCGGCCTT 
ATTCTCTGGC TGGCTCTGGA CACAGCTCAA AGGCCAGAGC AGCTGATCTC CTTTGCGGGA 
ATCTGCATGT TCATCCTCAT CCTCTTTGCC TGCTCCAAAC ACCACAGTGC GGTGAGCTGG 
AGGACAGTGT TTTGGGGCCT GGGCCTTCAG TTCGTCTTTG GGATTTTGGT CATCAGAACT 
GAACCTGGAT TTAATGCATT TCAATGGCTG GGAGATCAGA TCCAGATTTT CCTGGCATAT 
ACTGTAGAAG GATCCAGTTT TGTTTTTGGT GACACACTGG TCCAAAGTGT TTTTGCTTTT 
CAGTCTCTCC CAATCATTAT TTTCTTCGGA TGTGTGATGT CTATTCTCTA CTACCTGGGC 
CTTGTGCAGT GGGTGATCCA GAAGATTGCC TGGTTCCTGC AAATCACCAT GGGTACCACA 
GCTGCAGAGA CCCTGGCCGT GGCAGGAAAC ATCTTTGTGG GAATGACAGA GGCACCTCTG 
CTCATCCGTC CCTACCTTGC AGACATGACC CTCTCTGAAA TCCATGCAGT GATGACTGGA 
GGCTTTGCTA CTATAGCAGG CACAGTGTTG GGAGCCTTCA TATCCTTTGG GATTGATGCA 
TCATCCTTGA TTTCTGCCTC AGTGATGGCT GCCCCTTGTG CACTTGCCTT GTCCAAACTG 
GTATATCCAG AAGTAGAAGA GTCCAAGTTC AAGAGCAAGG AGGGAGTGAA GCTGCCTCGA 
GGGGAAGAGA GGAATATTCT GGAAGCCGCG AGCAACGGAG CCACAGATGC CATAGCGCTT 
GTTGCTAATG TTGCAGCCAA CCTGATTGCC TTTCTGGCTG TATTGGCCTT CATCAATTCT 
ACCCTTTCCT GGCTGGGGGA AATGGTGGAT ATCCATGGAC TCACTTTCCA GGTCATCTGC 
TCCTATGTCC TAAGGCCAAT GGTTTTCATG ATGGGTGTAC AGTGGGCAGA CTGCCCATTG 
GTGGCTGAGA TAGTGGGAGT CAAATTCTTC ATAAATGAAT TTGTGGCCTA TCAACAACTG 
TCTCAGTACA AGAACAAACG TCTCTCTGGA GTCGAAGAGT GGATCAATGG CGAGAAACAG 
TGGATTTCTG TGAAGGCTGA AATCATTGCA ACATTTTCTC TCTGTGGATT TGCCAATCTT 
ACTTCCATAG GGATCACACT GGGAGGCTTG ACATCCATGG TTCCCCAACG GAAGAGTGAC 
TTGTGCAAGC TTGTGGTCAG GGCCCTCTTC ACAGGTGCCT GTGTTTCCTT CATCAGTGCC 
TGTATGGCAG GAATCCTCTA TGTTCCCAGA GGCGCTGAAA CTGACTGTGT CTCATTCTTA 
AACACAAATT TCACCAACAG AACCTATGAG ACATATGTGT GCTGCAGAGA GCTCTTCCAG 
AGTACTTTGC TGAATGGCAC CAACATGCCT TCTTTCTCAG GCCCCTGGCA AGACAAGGAG 
TCCAGCCTCA GGAATCTTGC TAAATGCTGT GACCTCTATA CCAGCACTGT GTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localization of nucleoside transporters in rat epididymis.
Journal of biochemical and molecular toxicology31(8)(2017 Aug)
Klein DM,Harding MC,Crowther MK,Cherrington NJ

rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.
FEBS letters588(23)4382-9(2014 Nov)
Pinilla-Macua I,Claudio-Montero A,Pastor-Anglada M

Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2?in differentiated neuronal PC12 cells.
The Biochemical journal454(3)437-45(2013 Sep)
Medina-Pulido L,Molina-Arcas M,Justicia C,Soriano E,Burgaya F,Planas AM,Pastor-Anglada M

Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.
The Biochemical journal442(3)517-25(2012 Mar)
Pinilla-Macua I,Casado FJ,Pastor-Anglada M

Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.
Drug metabolism and pharmacokinetics27(4)439-46(2012)
Nishimura T,Chishu T,Tomi M,Nakamura R,Sato K,Kose N,Sai Y,Nakashima E

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Slc28a2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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