Prmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prmt1
Entrez Gene ID	60421
Full Name	protein arginine methyltransferase 1
Synonyms	Hrmt1l2
General protein information	Preferred Names protein arginine methyltransferase 1 Names protein arginine N-methyltransferase 1 heterogeneous nuclear ribonucleoproteins methyltransferase-like 2 histone-arginine N-methyltransferase PRMT1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	catalyzes protein methyltransferase modification of arginine residues [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229146.3	XP_006229208.1	protein arginine N-methyltransferase 1 isoform X3
XM_006229144.3	XP_006229206.1	protein arginine N-methyltransferase 1 isoform X1
XM_006229145.3	XP_006229207.1	protein arginine N-methyltransferase 1 isoform X2
NM_024363.1	NP_077339.1	protein arginine N-methyltransferase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Art1	NP_650017.1
Neurospora crassa	NCU07459	XP_963910.1
Arabidopsis thaliana (thale cress)	PRMT1A	NP_179557.1
Homo sapiens (human)	PRMT1	NP_001527.3
	LOC719419	XP_001113609.2
Magnaporthe oryzae (rice blast fungus)	MGG_04584	XP_003713607.1
Oryza sativa (rice)	Os09g0359800	NP_001062975.1
Canis lupus familiaris (dog)	PRMT1	XP_851543.1
Mus musculus (house mouse)	Prmt1	NP_062804.1
Eremothecium gossypii	AGOS_AAR190W	NP_982733.1
Danio rerio (zebrafish)	prmt1	NP_956944.2
Caenorhabditis elegans (roundworm)	prmt-1	NP_507909.1
Saccharomyces cerevisiae (baker's yeast)	HMT1	NP_009590.1
Kluyveromyces lactis	KLLA0B07117g	XP_451847.1
Bos taurus (cattle)	PRMT1	NP_001015624.1
Rattus norvegicus (Norway rat)	Prmt1	NP_077339.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003462	XP_311750.4
Schizosaccharomyces pombe (fission yeast)	rmt1	NP_594825.2
Arabidopsis thaliana (thale cress)	PRMT11	NP_194680.1
Xenopus tropicalis (tropical clawed frog)	prmt1	NP_001005629.2

Gene Ontology
Bibliography

Related articles in PubMed

Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats.
Xiao HB, Sui GG, Lu XY, Sun ZL
Inflammation41(1)299-306(2018 Feb)

PRMT1 promotes hyperglycemia in a FoxO1-dependent manner, affecting glucose metabolism, during hypobaric hypoxia exposure, in rat model.
Bayen S, Saini S, Gaur P, Duraisamy AJ, Kumar Sharma A, Pal K, Vats P, Singh SB
Endocrine59(1)151-163(2018 Jan)

FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.
Akter KA, Mansour MA, Hyodo T, Senga T
The international journal of biochemistry & cell biology841-13(2017 03)

Novel helical assembly in arginine methyltransferase 8.
Toma-Fukai S, Kim JD, Park KE, Kuwabara N, Shimizu N, Krayukhina E, Uchiyama S, Fukamizu A, Shimizu T
Journal of molecular biology428(6)1197-1208(2016 Mar)

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
Zhang L, Tran NT, Su H, Wang R, Lu Y, Tang H, Aoyagi S, Guo A, Khodadadi-Jamayran A, Zhou D, Qian K, Hricik T, C?t? J, Han X, Zhou W, Laha S, Abdel-Wahab O, Levine RL, Raffel G, Liu Y, Chen D, Li H, Townes T, Wang H, Deng H, Zheng YG, Leslie C, Luo M, Zhao X
eLife4(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TGF-beta-induced PRMT1 expression participates in fibroblast proliferation and chronic airway inflammation in ovalbumin-induced asthma.
Title: PRMT1 Upregulated by Epithelial Proinflammatory Cytokines Participates in COX2 Expression in Fibroblasts and Chronic Antigen-Induced Pulmonary Inflammation.

higher energy barrier of SDMA formation (DeltaDeltaG(double dagger) = 3.2 kcal/mol as compared with ADMA) may explain the small amount of SDMA generated by M48F-PRMT1
Title: A remodeled protein arginine methyltransferase 1 (PRMT1) generates symmetric dimethylarginine.

The detailed mechanism of PRMT1-catalyzed asymmetric dimethylation, is reported.
Title: Theoretical insights into catalytic mechanism of protein arginine methyltransferase 1.

Study reports that PRMT1 methylates a multisite peptide substrate in a nonstochastic manner, with less C-terminal preference, consistent with the methylation patterns observed in vivo. With a single targeted arginine, PRMT1 catalyzed the dimethylation in a semiprocessive manner.
Title: Substrate-induced control of product formation by protein arginine methyltransferase 1.

PRMT1 and DDAH-1, responsible for the biosynthesis and degradation of asymmetric dimethylarginine respectively, are expressed in erythrocytes, leukocytes, and platelets.
Title: Asymmetric dimethylarginine, an endogenous NOS inhibitor, is actively metabolized in rat erythrocytes.

The following Prmt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prmt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13575	NM_024363.1 Latest version!	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa22776	XM_006229144.3 Latest version!	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22777	XM_006229145.3 Latest version!	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22778	XM_006229146.3 Latest version!	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prmt1 ( NM_024363.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077339.1 Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), mRNA.

Price & Availability↑

CloneID	ORa13575
Clone ID Related Accession (Same CDS sequence)	NM_024363.1
Accession Version	NM_024363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	protein arginine N-methyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U60882.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U60882.1, FQ219843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG CCGAGGCCGC GAACTGCATC ATGGAGGTTT CCTGTGGCCA AGCAGAAAGT 
AGTGAGAAGC CCAATGCTGA GGACATGACA TCCAAAGACT ACTACTTTGA CTCCTATGCC 
CACTTTGGCA TCCACGAGGA GATGCTAAAG GATGAGGTGC GAACCCTCAC GTACCGCAAC 
TCCATGTTTC ACAATCGGCA TCTCTTCAAA GACAAGGTGG TGCTGGATGT GGGCTCGGGC 
ACTGGCATCC TCTGCATGTT CGCTGCCAAG GCAGGGGCCC GCAAGGTCAT TGGGATCGAG 
TGCTCCAGTA TCTCTGATTA TGCTGTGAAG ATTGTCAAAG CCAATAAGTT AGACCACGTG 
GTGACCATCA TCAAGGGCAA GGTGGAGGAG GTGGAGCTGC CTGTGGAGAA GGTGGACATC 
ATCATCAGCG AGTGGATGGG TTATTGCCTC TTCTATGAGT CCATGCTCAA CACTGTGCTG 
CACGCTCGTG ACAAGTGGCT GGCACCTGAT GGCCTCATCT TCCCAGACCG AGCCACCCTG 
TATGTGACAG CCATTGAGGA CCGACAGTAT AAAGACTACA AGATCCACTG GTGGGAGAAT 
GTATATGGCT TTGATATGTC CTGCATTAAA GACGTGGCCA TCAAGGAGCC CCTGGTGGAC 
GTGGTGGACC CAAAGCAGCT GGTCACCAAC GCCTGCCTCA TAAAGGAGGT GGACATCTAC 
ACAGTCAAGG TGGAGGACCT GACCTTCACC TCCCCGTTTT GTCTGCAAGT GAAGAGGAAT 
GACTATGTGC ATGCACTAGT GGCCTACTTC AACATCGAGT TCACCCGATG CCACAAGAGG 
ACCGGCTTCT CTACCAGTCC TGAGTCTCCA TACACACATT GGAAGCAGAC TGTGTTCTAC 
ATGGAGGACT ACCTAACAGT GAAGACCGGC GAGGAGATTT TTGGCACTAT TGGAATGAGG 
CCCAACGCCA AAAACAATCG TGACTTGGAC TTTACCATCG ACCTGGACTT CAAGGGTCAG 
CTGTGTGAGC TCTCTTGTTC CACCGACTAC CGGATGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077339.1
CDS	3..1064
Translation	MAAAEAANCIMEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

Target ORF information:

RefSeq Version	NM_024363.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024363.1

ORF Insert Sequence:

ATGGCGGCAG CCGAGGCCGC GAACTGCATC ATGGAGGTTT CCTGTGGCCA AGCAGAAAGT 
AGTGAGAAGC CCAATGCTGA GGACATGACA TCCAAAGACT ACTACTTTGA CTCCTATGCC 
CACTTTGGCA TCCACGAGGA GATGCTAAAG GATGAGGTGC GAACCCTCAC GTACCGCAAC 
TCCATGTTTC ACAATCGGCA TCTCTTCAAA GACAAGGTGG TGCTGGATGT GGGCTCGGGC 
ACTGGCATCC TCTGCATGTT CGCTGCCAAG GCAGGGGCCC GCAAGGTCAT TGGGATCGAG 
TGCTCCAGTA TCTCTGATTA TGCTGTGAAG ATTGTCAAAG CCAATAAGTT AGACCACGTG 
GTGACCATCA TCAAGGGCAA GGTGGAGGAG GTGGAGCTGC CTGTGGAGAA GGTGGACATC 
ATCATCAGCG AGTGGATGGG TTATTGCCTC TTCTATGAGT CCATGCTCAA CACTGTGCTG 
CACGCTCGTG ACAAGTGGCT GGCACCTGAT GGCCTCATCT TCCCAGACCG AGCCACCCTG 
TATGTGACAG CCATTGAGGA CCGACAGTAT AAAGACTACA AGATCCACTG GTGGGAGAAT 
GTATATGGCT TTGATATGTC CTGCATTAAA GACGTGGCCA TCAAGGAGCC CCTGGTGGAC 
GTGGTGGACC CAAAGCAGCT GGTCACCAAC GCCTGCCTCA TAAAGGAGGT GGACATCTAC 
ACAGTCAAGG TGGAGGACCT GACCTTCACC TCCCCGTTTT GTCTGCAAGT GAAGAGGAAT 
GACTATGTGC ATGCACTAGT GGCCTACTTC AACATCGAGT TCACCCGATG CCACAAGAGG 
ACCGGCTTCT CTACCAGTCC TGAGTCTCCA TACACACATT GGAAGCAGAC TGTGTTCTAC 
ATGGAGGACT ACCTAACAGT GAAGACCGGC GAGGAGATTT TTGGCACTAT TGGAATGAGG 
CCCAACGCCA AAAACAATCG TGACTTGGAC TTTACCATCG ACCTGGACTT CAAGGGTCAG 
CTGTGTGAGC TCTCTTGTTC CACCGACTAC CGGATGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prmt1 ( XM_006229144.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229206.1 Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22776
Clone ID Related Accession (Same CDS sequence)	XM_006229144.3
Accession Version	XM_006229144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein arginine N-methyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAG CCGAGGCCGC GAACTGCATC ATGGAGAATT TTGTAGCCAC CTTGGCTAAT 
GGGATGAGCC TCCAGCCGCC TCTTGAAGAA GTTTCCTGTG GCCAAGCAGA AAGTAGTGAG 
AAGCCCAATG CTGAGGACAT GACATCCAAA GACTACTACT TTGACTCCTA TGCCCACTTT 
GGCATCCACG AGGAGATGCT AAAGGATGAG GTGCGAACCC TCACGTACCG CAACTCCATG 
TTTCACAATC GGCATCTCTT CAAAGACAAG GTGGTGCTGG ATGTGGGCTC GGGCACTGGC 
ATCCTCTGCA TGTTCGCTGC CAAGGCAGGG GCCCGCAAGG TCATTGGGAT CGAGTGCTCC 
AGTATCTCTG ATTATGCTGT GAAGATTGTC AAAGCCAATA AGTTAGACCA CGTGGTGACC 
ATCATCAAGG GCAAGGTGGA GGAGGTGGAG CTGCCTGTGG AGAAGGTGGA CATCATCATC 
AGCGAGTGGA TGGGTTATTG CCTCTTCTAT GAGTCCATGC TCAACACTGT GCTGCACGCT 
CGTGACAAGT GGCTGGCACC TGATGGCCTC ATCTTCCCAG ACCGAGCCAC CCTGTATGTG 
ACAGCCATTG AGGACCGACA GTATAAAGAC TACAAGATCC ACTGGTGGGA GAATGTATAT 
GGCTTTGATA TGTCCTGCAT TAAAGACGTG GCCATCAAGG AGCCCCTGGT GGACGTGGTG 
GACCCAAAGC AGCTGGTCAC CAACGCCTGC CTCATAAAGG AGGTGGACAT CTACACAGTC 
AAGGTGGAGG ACCTGACCTT CACCTCCCCG TTTTGTCTGC AAGTGAAGAG GAATGACTAT 
GTGCATGCAC TAGTGGCCTA CTTCAACATC GAGTTCACCC GATGCCACAA GAGGACCGGC 
TTCTCTACCA GTCCTGAGTC TCCATACACA CATTGGAAGC AGACTGTGTT CTACATGGAG 
GACTACCTAA CAGTGAAGAC CGGCGAGGAG ATTTTTGGCA CTATTGGAAT GAGGCCCAAC 
GCCAAAAACA ATCGTGACTT GGACTTTACC ATCGACCTGG ACTTCAAGGG TCAGCTGTGT 
GAGCTCTCTT GTTCCACCGA CTACCGGATG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229206.1
CDS	72..1187
Translation	MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

Target ORF information:

RefSeq Version	XM_006229144.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229144.3

ORF Insert Sequence:

ATGGCGGCAG CCGAGGCCGC GAACTGCATC ATGGAGAATT TTGTAGCCAC CTTGGCTAAT 
GGGATGAGCC TCCAGCCGCC TCTTGAAGAA GTTTCCTGTG GCCAAGCAGA AAGTAGTGAG 
AAGCCCAATG CTGAGGACAT GACATCCAAA GACTACTACT TTGACTCCTA TGCCCACTTT 
GGCATCCACG AGGAGATGCT AAAGGATGAG GTGCGAACCC TCACGTACCG CAACTCCATG 
TTTCACAATC GGCATCTCTT CAAAGACAAG GTGGTGCTGG ATGTGGGCTC GGGCACTGGC 
ATCCTCTGCA TGTTCGCTGC CAAGGCAGGG GCCCGCAAGG TCATTGGGAT CGAGTGCTCC 
AGTATCTCTG ATTATGCTGT GAAGATTGTC AAAGCCAATA AGTTAGACCA CGTGGTGACC 
ATCATCAAGG GCAAGGTGGA GGAGGTGGAG CTGCCTGTGG AGAAGGTGGA CATCATCATC 
AGCGAGTGGA TGGGTTATTG CCTCTTCTAT GAGTCCATGC TCAACACTGT GCTGCACGCT 
CGTGACAAGT GGCTGGCACC TGATGGCCTC ATCTTCCCAG ACCGAGCCAC CCTGTATGTG 
ACAGCCATTG AGGACCGACA GTATAAAGAC TACAAGATCC ACTGGTGGGA GAATGTATAT 
GGCTTTGATA TGTCCTGCAT TAAAGACGTG GCCATCAAGG AGCCCCTGGT GGACGTGGTG 
GACCCAAAGC AGCTGGTCAC CAACGCCTGC CTCATAAAGG AGGTGGACAT CTACACAGTC 
AAGGTGGAGG ACCTGACCTT CACCTCCCCG TTTTGTCTGC AAGTGAAGAG GAATGACTAT 
GTGCATGCAC TAGTGGCCTA CTTCAACATC GAGTTCACCC GATGCCACAA GAGGACCGGC 
TTCTCTACCA GTCCTGAGTC TCCATACACA CATTGGAAGC AGACTGTGTT CTACATGGAG 
GACTACCTAA CAGTGAAGAC CGGCGAGGAG ATTTTTGGCA CTATTGGAAT GAGGCCCAAC 
GCCAAAAACA ATCGTGACTT GGACTTTACC ATCGACCTGG ACTTCAAGGG TCAGCTGTGT 
GAGCTCTCTT GTTCCACCGA CTACCGGATG CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prmt1 ( XM_006229145.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229207.1 Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22777
Clone ID Related Accession (Same CDS sequence)	XM_006229145.3
Accession Version	XM_006229145.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein arginine N-methyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGGCG AGGCCGCTGC TCCCTGGATG GTGCTCTGGG TTTCCTGTGG CCAAGCAGAA 
AGTAGTGAGA AGCCCAATGC TGAGGACATG ACATCCAAAG ACTACTACTT TGACTCCTAT 
GCCCACTTTG GCATCCACGA GGAGATGCTA AAGGATGAGG TGCGAACCCT CACGTACCGC 
AACTCCATGT TTCACAATCG GCATCTCTTC AAAGACAAGG TGGTGCTGGA TGTGGGCTCG 
GGCACTGGCA TCCTCTGCAT GTTCGCTGCC AAGGCAGGGG CCCGCAAGGT CATTGGGATC 
GAGTGCTCCA GTATCTCTGA TTATGCTGTG AAGATTGTCA AAGCCAATAA GTTAGACCAC 
GTGGTGACCA TCATCAAGGG CAAGGTGGAG GAGGTGGAGC TGCCTGTGGA GAAGGTGGAC 
ATCATCATCA GCGAGTGGAT GGGTTATTGC CTCTTCTATG AGTCCATGCT CAACACTGTG 
CTGCACGCTC GTGACAAGTG GCTGGCACCT GATGGCCTCA TCTTCCCAGA CCGAGCCACC 
CTGTATGTGA CAGCCATTGA GGACCGACAG TATAAAGACT ACAAGATCCA CTGGTGGGAG 
AATGTATATG GCTTTGATAT GTCCTGCATT AAAGACGTGG CCATCAAGGA GCCCCTGGTG 
GACGTGGTGG ACCCAAAGCA GCTGGTCACC AACGCCTGCC TCATAAAGGA GGTGGACATC 
TACACAGTCA AGGTGGAGGA CCTGACCTTC ACCTCCCCGT TTTGTCTGCA AGTGAAGAGG 
AATGACTATG TGCATGCACT AGTGGCCTAC TTCAACATCG AGTTCACCCG ATGCCACAAG 
AGGACCGGCT TCTCTACCAG TCCTGAGTCT CCATACACAC ATTGGAAGCA GACTGTGTTC 
TACATGGAGG ACTACCTAAC AGTGAAGACC GGCGAGGAGA TTTTTGGCAC TATTGGAATG 
AGGCCCAACG CCAAAAACAA TCGTGACTTG GACTTTACCA TCGACCTGGA CTTCAAGGGT 
CAGCTGTGTG AGCTCTCTTG TTCCACCGAC TACCGGATGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229207.1
CDS	280..1344
Translation	MVGEAAAPWMVLWVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

Target ORF information:

RefSeq Version	XM_006229145.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229145.3

ORF Insert Sequence:

ATGGTAGGCG AGGCCGCTGC TCCCTGGATG GTGCTCTGGG TTTCCTGTGG CCAAGCAGAA 
AGTAGTGAGA AGCCCAATGC TGAGGACATG ACATCCAAAG ACTACTACTT TGACTCCTAT 
GCCCACTTTG GCATCCACGA GGAGATGCTA AAGGATGAGG TGCGAACCCT CACGTACCGC 
AACTCCATGT TTCACAATCG GCATCTCTTC AAAGACAAGG TGGTGCTGGA TGTGGGCTCG 
GGCACTGGCA TCCTCTGCAT GTTCGCTGCC AAGGCAGGGG CCCGCAAGGT CATTGGGATC 
GAGTGCTCCA GTATCTCTGA TTATGCTGTG AAGATTGTCA AAGCCAATAA GTTAGACCAC 
GTGGTGACCA TCATCAAGGG CAAGGTGGAG GAGGTGGAGC TGCCTGTGGA GAAGGTGGAC 
ATCATCATCA GCGAGTGGAT GGGTTATTGC CTCTTCTATG AGTCCATGCT CAACACTGTG 
CTGCACGCTC GTGACAAGTG GCTGGCACCT GATGGCCTCA TCTTCCCAGA CCGAGCCACC 
CTGTATGTGA CAGCCATTGA GGACCGACAG TATAAAGACT ACAAGATCCA CTGGTGGGAG 
AATGTATATG GCTTTGATAT GTCCTGCATT AAAGACGTGG CCATCAAGGA GCCCCTGGTG 
GACGTGGTGG ACCCAAAGCA GCTGGTCACC AACGCCTGCC TCATAAAGGA GGTGGACATC 
TACACAGTCA AGGTGGAGGA CCTGACCTTC ACCTCCCCGT TTTGTCTGCA AGTGAAGAGG 
AATGACTATG TGCATGCACT AGTGGCCTAC TTCAACATCG AGTTCACCCG ATGCCACAAG 
AGGACCGGCT TCTCTACCAG TCCTGAGTCT CCATACACAC ATTGGAAGCA GACTGTGTTC 
TACATGGAGG ACTACCTAAC AGTGAAGACC GGCGAGGAGA TTTTTGGCAC TATTGGAATG 
AGGCCCAACG CCAAAAACAA TCGTGACTTG GACTTTACCA TCGACCTGGA CTTCAAGGGT 
CAGCTGTGTG AGCTCTCTTG TTCCACCGAC TACCGGATGC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prmt1 ( XM_006229146.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229208.1 Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X3, mRNA.

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CloneID	ORa22778
Clone ID Related Accession (Same CDS sequence)	XM_006229146.3
Accession Version	XM_006229146.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein arginine N-methyltransferase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229146.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCCT GTGGCCAAGC AGAAAGTAGT GAGAAGCCCA ATGCTGAGGA CATGACATCC 
AAAGACTACT ACTTTGACTC CTATGCCCAC TTTGGCATCC ACGAGGAGAT GCTAAAGGAT 
GAGGTGCGAA CCCTCACGTA CCGCAACTCC ATGTTTCACA ATCGGCATCT CTTCAAAGAC 
AAGGTGGTGC TGGATGTGGG CTCGGGCACT GGCATCCTCT GCATGTTCGC TGCCAAGGCA 
GGGGCCCGCA AGGTCATTGG GATCGAGTGC TCCAGTATCT CTGATTATGC TGTGAAGATT 
GTCAAAGCCA ATAAGTTAGA CCACGTGGTG ACCATCATCA AGGGCAAGGT GGAGGAGGTG 
GAGCTGCCTG TGGAGAAGGT GGACATCATC ATCAGCGAGT GGATGGGTTA TTGCCTCTTC 
TATGAGTCCA TGCTCAACAC TGTGCTGCAC GCTCGTGACA AGTGGCTGGC ACCTGATGGC 
CTCATCTTCC CAGACCGAGC CACCCTGTAT GTGACAGCCA TTGAGGACCG ACAGTATAAA 
GACTACAAGA TCCACTGGTG GGAGAATGTA TATGGCTTTG ATATGTCCTG CATTAAAGAC 
GTGGCCATCA AGGAGCCCCT GGTGGACGTG GTGGACCCAA AGCAGCTGGT CACCAACGCC 
TGCCTCATAA AGGAGGTGGA CATCTACACA GTCAAGGTGG AGGACCTGAC CTTCACCTCC 
CCGTTTTGTC TGCAAGTGAA GAGGAATGAC TATGTGCATG CACTAGTGGC CTACTTCAAC 
ATCGAGTTCA CCCGATGCCA CAAGAGGACC GGCTTCTCTA CCAGTCCTGA GTCTCCATAC 
ACACATTGGA AGCAGACTGT GTTCTACATG GAGGACTACC TAACAGTGAA GACCGGCGAG 
GAGATTTTTG GCACTATTGG AATGAGGCCC AACGCCAAAA ACAATCGTGA CTTGGACTTT 
ACCATCGACC TGGACTTCAA GGGTCAGCTG TGTGAGCTCT CTTGTTCCAC CGACTACCGG 
ATGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229208.1
CDS	429..1457
Translation	MVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLHARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

Target ORF information:

RefSeq Version	XM_006229146.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein arginine methyltransferase 1 (Prmt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229146.3

ORF Insert Sequence:

ATGGTTTCCT GTGGCCAAGC AGAAAGTAGT GAGAAGCCCA ATGCTGAGGA CATGACATCC 
AAAGACTACT ACTTTGACTC CTATGCCCAC TTTGGCATCC ACGAGGAGAT GCTAAAGGAT 
GAGGTGCGAA CCCTCACGTA CCGCAACTCC ATGTTTCACA ATCGGCATCT CTTCAAAGAC 
AAGGTGGTGC TGGATGTGGG CTCGGGCACT GGCATCCTCT GCATGTTCGC TGCCAAGGCA 
GGGGCCCGCA AGGTCATTGG GATCGAGTGC TCCAGTATCT CTGATTATGC TGTGAAGATT 
GTCAAAGCCA ATAAGTTAGA CCACGTGGTG ACCATCATCA AGGGCAAGGT GGAGGAGGTG 
GAGCTGCCTG TGGAGAAGGT GGACATCATC ATCAGCGAGT GGATGGGTTA TTGCCTCTTC 
TATGAGTCCA TGCTCAACAC TGTGCTGCAC GCTCGTGACA AGTGGCTGGC ACCTGATGGC 
CTCATCTTCC CAGACCGAGC CACCCTGTAT GTGACAGCCA TTGAGGACCG ACAGTATAAA 
GACTACAAGA TCCACTGGTG GGAGAATGTA TATGGCTTTG ATATGTCCTG CATTAAAGAC 
GTGGCCATCA AGGAGCCCCT GGTGGACGTG GTGGACCCAA AGCAGCTGGT CACCAACGCC 
TGCCTCATAA AGGAGGTGGA CATCTACACA GTCAAGGTGG AGGACCTGAC CTTCACCTCC 
CCGTTTTGTC TGCAAGTGAA GAGGAATGAC TATGTGCATG CACTAGTGGC CTACTTCAAC 
ATCGAGTTCA CCCGATGCCA CAAGAGGACC GGCTTCTCTA CCAGTCCTGA GTCTCCATAC 
ACACATTGGA AGCAGACTGT GTTCTACATG GAGGACTACC TAACAGTGAA GACCGGCGAG 
GAGATTTTTG GCACTATTGG AATGAGGCCC AACGCCAAAA ACAATCGTGA CTTGGACTTT 
ACCATCGACC TGGACTTCAA GGGTCAGCTG TGTGAGCTCT CTTGTTCCAC CGACTACCGG 
ATGCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elevated Levels of ADMA Are Associated with Lower DDAH2 and Higher PRMT1 in LPS-Induced Endometritis Rats.
Inflammation41(1)299-306(2018 Feb)
Xiao HB,Sui GG,Lu XY,Sun ZL

PRMT1 promotes hyperglycemia in a FoxO1-dependent manner, affecting glucose metabolism, during hypobaric hypoxia exposure, in rat model.
Endocrine59(1)151-163(2018 Jan)
Bayen S,Saini S,Gaur P,Duraisamy AJ,Kumar Sharma A,Pal K,Vats P,Singh SB

FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.
The international journal of biochemistry & cell biology841-13(2017 03)
Akter KA,Mansour MA,Hyodo T,Senga T

Novel helical assembly in arginine methyltransferase 8.
Journal of molecular biology428(6)1197-1208(2016 Mar)
Toma-Fukai S,Kim JD,Park KE,Kuwabara N,Shimizu N,Krayukhina E,Uchiyama S,Fukamizu A,Shimizu T

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
eLife4(2015 Nov)
Zhang L,Tran NT,Su H,Wang R,Lu Y,Tang H,Aoyagi S,Guo A,Khodadadi-Jamayran A,Zhou D,Qian K,Hricik T,C?t? J,Han X,Zhou W,Laha S,Abdel-Wahab O,Levine RL,Raffel G,Liu Y,Chen D,Li H,Townes T,Wang H,Deng H,Zheng YG,Leslie C,Luo M,Zhao X

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Prmt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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