Nsf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nsf
Entrez Gene ID	60355
Full Name	N-ethylmaleimide sensitive factor, vesicle fusing ATPase
General protein information	Preferred Names N-ethylmaleimide sensitive factor, vesicle fusing ATPase Names vesicle-fusing ATPase N-ethylmaleimide-sensitive factor N-ethylmaleimide-sensitive fusion protein NEM-sensitive fusion protein vesicular-fusion protein NSF
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	binds AMPA receptor GluR2 subunit and acts to disrupt GluR2-PICK1 interactions; may play a role in AMPA receptor trafficking and stabilization [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021748.1	NP_068516.1	vesicle-fusing ATPase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NSF	XP_511626.3
Canis lupus familiaris (dog)	NSF	XP_548044.2
Mus musculus (house mouse)	Nsf	NP_032766.2
Drosophila melanogaster (fruit fly)	comt	NP_001259506.1
Saccharomyces cerevisiae (baker's yeast)	SEC18	NP_009636.3
Gallus gallus (chicken)	NSF	NP_001019627.1
Danio rerio (zebrafish)	nsfa	NP_001037793.1
Neurospora crassa	NCU03387	XP_956834.1
Xenopus tropicalis (tropical clawed frog)	nsf	NP_001072788.1
Bos taurus (cattle)	NSF	XP_002696103.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012684	XP_307148.3
Schizosaccharomyces pombe (fission yeast)	sec18	NP_594690.1
Magnaporthe oryzae (rice blast fungus)	MGG_02418	XP_003709204.1
	NSF	XP_002800596.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076603.1
Caenorhabditis elegans (roundworm)	nsf-1	NP_001076604.1
Homo sapiens (human)	NSF	NP_006169.2
Rattus norvegicus (Norway rat)	Nsf	NP_068516.1
Drosophila melanogaster (fruit fly)	Nsf2	NP_788676.1
Kluyveromyces lactis	KLLA0C14520g	XP_452850.1
Eremothecium gossypii	AGOS_AER169C	NP_985027.1

Gene Ontology
Bibliography

Related articles in PubMed

Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus.
Xiong H, Cass? F, Zhou M, Xiong ZQ, Joels M, Martin S, Krugers HJ
Hippocampus26(7)848-56(2016 Jul)

Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia.
G?mez-Chac?n B, G?miz F, Foster TC, Gallo M
Neural plasticity20162726745(2016)

Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk.
Qu Y, Ma KN, Li XZ
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban34(6)801-7(2014 Dec)

The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2.
Migues PV, Hardt O, Finnie P, Wang YW, Nader K
Hippocampus24(9)1112-9(2014 Sep)

Interactions between N-ethylmaleimide-sensitive factor and GluR2 in the nucleus accumbens contribute to the expression of locomotor sensitization to cocaine.
Lu HF, Wu PF, Yang YJ, Xiao W, Fan J, Liu J, Li YL, Luo Y, Hu ZL, Jin Y, Wang F, Chen JG
The Journal of neuroscience : the official journal of the Society for Neuroscience34(10)3493-508(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that glucocorticoid hormones increase AMPA receptor mediated synaptic transmission and synaptic insertion of AMPA receptors via a mechanism that requires NSF/GluA2 interaction. This may contribute to the memory enhancing effects of glucocorticoid hormones.
Title: Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus.

We have found increased expression of NSF gene in basolateral amygdala and perirhinal cortex in groups of rats undergoing taste habituation.
Title: Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia.

TCST increases the expression of NSF in the hippocampus of adult rats with focal cerebral ischemia, which may contribute to the protection of the injured brain.
Title: Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk.

Results indicate that the persistence of long-term memory depends on the maintenance of a steady-state level of synaptic GluA2/AMPARs, which requires the interaction of NSF with GluA2
Title: The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2.

NSF-GluR2 interactions increase in the nucleus accumbens after cocaine withdrawal.
Title: Interactions between N-ethylmaleimide-sensitive factor and GluR2 in the nucleus accumbens contribute to the expression of locomotor sensitization to cocaine.

The following Nsf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13217	NM_021748.1 Latest version!	Rattus norvegicus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsf ( NM_021748.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068516.1 Rattus norvegicus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf), mRNA.

Price & Availability↑

CloneID	ORa13217
Clone ID Related Accession (Same CDS sequence)	NM_021748.1
Accession Version	NM_021748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	vesicle-fusing ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF189019.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ211783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGGCC GGACTATGCA AGCTGCGAGA TGCCCTACTG ATGAATTGTC TCTAAGCAAC 
TGTGCAGTTG TGAACGAAAA GGATTACCAG TCTGGCCAGC ATGTGATGGT GAGGACGTCC 
CCCAACCACA AGTACATATT TACGCTGCGG ACCCATCCGT CCGTGGTTCC CGGATGCATC 
GCGTTCAGCT TACCCCAGCG AAAATGGGCT GGACTTTCTA TTGGGCAGGA CATAGAAGTC 
GCCTTGTATT CGTTTGACAA AGCCAAGCAG TGCATCGGCA CAATGACCAT CGAGATCGAC 
TTCCTGCAGA AAAAGAACAT CGACTCCAAC CCTTACGACA CCGACAAGAT GGCTGCCGAG 
TTCATCCAGC AGTTCAACCA CCAGGCCTTC TCAGTGGGAC AGCAGCTTGT CTTTAGCTTC 
AATGATAAGC TCTTTGGATT GCTGGTGAAG GACATCGAAG CCATGGATCC CAGCATCCTG 
AAGGGAGAGC CTGCGTCAGG TAAAAGGCAG AAGATTGAAG TAGGACTGGT CGTTGGAAAC 
AGTCAAGTTG CATTTGAAAA AGCAGAAAAC TCATCACTCA ATCTTATTGG CAAGGCTAAA 
ACCAAGGAAA ATCGCCAGTC TATCATCAAT CCCGACTGGA ACTTTGAGAA AATGGGGATC 
GGCGGTCTGG ATAAGGAGTT TTCAGATATT TTCCGACGAG CATTTGCTTC TCGGGTGTTC 
CCTCCGGAGA TCGTGGAACA AATGGGTTGC AAACATGTTA AAGGCATCCT GTTATACGGA 
CCCCCTGGTT GTGGTAAGAC TCTCTTGGCT CGACAGATTG GCAAGATGCT GAATGCAAGA 
GAGCCCAAGG TGGTCAATGG GCCGGAAATC CTTAACAAAT ATGTGGGAGA ATCAGAGGCC 
AACATCCGCA AGCTCTTTGC TGATGCAGAA GAGGAGCAGA GACGGCTCGG TGCCAACAGC 
GGCCTGCACA TCATCATCTT TGATGAAATC GACGCCATCT GCAAGCAGAG AGGGAGCATG 
GCCGGCAGCA CCGGGGTGCA CGACACTGTG GTCAACCAGC TGCTGTCCAA GATCGATGGC 
GTCGAGCAGC TGAACAACAT CCTCGTTATT GGAATGACCA ATCGACCAGA TTTGATTGAT 
GAGGCTCTCC TTCGACCTGG AAGACTGGAA GTTAAGATGG AGATAGGCTT ACCAGATGAG 
AAGGGCCGAC TGCAGATCCT TCACATCCAC ACAGCAAGGA TGCGAGGGCA CCAGCTACTG 
TCTGCGGATG TGGACATCAA GGAACTGGCT GTGGAGACTA AGAATTTCAG TGGCGCCGAG 
CTGGAGGGCC TGGTGCGAGC AGCGCAGTCC ACAGCCATGA ACAGACACAT AAAGGCCAGC 
ACAAAAGTGG AAGTAGACAT GGAGAAGGCA GAGAGCCTGC AGGTGACAAG AGGGGACTTC 
CTCGCTTCCC TGGAGAATGA TATCAAACCG GCATTCGGCA CAAACCAAGA GGACTATGCA 
AGTTACATTA TGAATGGAAT CATCAAGTGG GGTGACCCCG TGACCCGAGT CCTCGACGAC 
GGAGAGCTGC TGGTCCAGCA GACTAAAAAC AGTGACCGCA CACCGCTGGT TAGCGTCCTT 
CTGGAAGGCC CTCCTCACAG CGGGAAGACT GCCCTCGCTG CAAAGATTGC CGAGGAATCA 
AACTTCCCCT TCATCAAGAT CTGCTCCCCC GATAAGATGA TTGGGTTCTC TGAGACAGCC 
AAGTGTCAGG CCATGAAGAA GATCTTTGAT GATGCTTACA AATCTCAGCT CAGTTGTGTG 
GTCGTGGATG ACATTGAGAG GTTGCTCGAT TACGTCCCCA TCGGCCCCCG GTTCTCCAAC 
CTGGTGTTAC AAGCTCTGCT CGTGTTACTG AAAAAGGCAC CTCCTCAGGG CCGCAAGCTT 
CTCATCATCG GGACCACCAG CCGCAAAGAT GTCCTGCAGG AAATGGAGAT GCTCAACGCC 
TTCAGCACCA CCATCCACGT GCCCAACATC GCCACAGGAG AGCAGCTGCT GGAAGCTCTG 
GAGCTTTTGG GCAACTTCAA AGATAAGGAA CGCACCACAA TTGCTCAGCA AGTCAAAGGG 
AAGAAAGTCT GGATAGGAAT CAAGAAGTTA CTAATGTTGA TTGAGATGTC CCTGCAGATG 
GACCCTGAGT ACCGTGTGAG GAAATTCTTG GCCCTCATGA GAGAAGAAGG CGCTAGTCCC 
CTGGACTTTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068516.1
CDS	5..2239
Translation	MAGRTMQAARCPTDELSLSNCAVVNEKDYQSGQHVMVRTSPNHKYIFTLRTHPSVVPGCIAFSLPQRKWAGLSIGQDIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNHQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALMREEGASPLDFD

Target ORF information:

RefSeq Version	NM_021748.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-ethylmaleimide sensitive factor, vesicle fusing ATPase (Nsf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021748.1

ORF Insert Sequence:

ATGGCGGGCC GGACTATGCA AGCTGCGAGA TGCCCTACTG ATGAATTGTC TCTAAGCAAC 
TGTGCAGTTG TGAACGAAAA GGATTACCAG TCTGGCCAGC ATGTGATGGT GAGGACGTCC 
CCCAACCACA AGTACATATT TACGCTGCGG ACCCATCCGT CCGTGGTTCC CGGATGCATC 
GCGTTCAGCT TACCCCAGCG AAAATGGGCT GGACTTTCTA TTGGGCAGGA CATAGAAGTC 
GCCTTGTATT CGTTTGACAA AGCCAAGCAG TGCATCGGCA CAATGACCAT CGAGATCGAC 
TTCCTGCAGA AAAAGAACAT CGACTCCAAC CCTTACGACA CCGACAAGAT GGCTGCCGAG 
TTCATCCAGC AGTTCAACCA CCAGGCCTTC TCAGTGGGAC AGCAGCTTGT CTTTAGCTTC 
AATGATAAGC TCTTTGGATT GCTGGTGAAG GACATCGAAG CCATGGATCC CAGCATCCTG 
AAGGGAGAGC CTGCGTCAGG TAAAAGGCAG AAGATTGAAG TAGGACTGGT CGTTGGAAAC 
AGTCAAGTTG CATTTGAAAA AGCAGAAAAC TCATCACTCA ATCTTATTGG CAAGGCTAAA 
ACCAAGGAAA ATCGCCAGTC TATCATCAAT CCCGACTGGA ACTTTGAGAA AATGGGGATC 
GGCGGTCTGG ATAAGGAGTT TTCAGATATT TTCCGACGAG CATTTGCTTC TCGGGTGTTC 
CCTCCGGAGA TCGTGGAACA AATGGGTTGC AAACATGTTA AAGGCATCCT GTTATACGGA 
CCCCCTGGTT GTGGTAAGAC TCTCTTGGCT CGACAGATTG GCAAGATGCT GAATGCAAGA 
GAGCCCAAGG TGGTCAATGG GCCGGAAATC CTTAACAAAT ATGTGGGAGA ATCAGAGGCC 
AACATCCGCA AGCTCTTTGC TGATGCAGAA GAGGAGCAGA GACGGCTCGG TGCCAACAGC 
GGCCTGCACA TCATCATCTT TGATGAAATC GACGCCATCT GCAAGCAGAG AGGGAGCATG 
GCCGGCAGCA CCGGGGTGCA CGACACTGTG GTCAACCAGC TGCTGTCCAA GATCGATGGC 
GTCGAGCAGC TGAACAACAT CCTCGTTATT GGAATGACCA ATCGACCAGA TTTGATTGAT 
GAGGCTCTCC TTCGACCTGG AAGACTGGAA GTTAAGATGG AGATAGGCTT ACCAGATGAG 
AAGGGCCGAC TGCAGATCCT TCACATCCAC ACAGCAAGGA TGCGAGGGCA CCAGCTACTG 
TCTGCGGATG TGGACATCAA GGAACTGGCT GTGGAGACTA AGAATTTCAG TGGCGCCGAG 
CTGGAGGGCC TGGTGCGAGC AGCGCAGTCC ACAGCCATGA ACAGACACAT AAAGGCCAGC 
ACAAAAGTGG AAGTAGACAT GGAGAAGGCA GAGAGCCTGC AGGTGACAAG AGGGGACTTC 
CTCGCTTCCC TGGAGAATGA TATCAAACCG GCATTCGGCA CAAACCAAGA GGACTATGCA 
AGTTACATTA TGAATGGAAT CATCAAGTGG GGTGACCCCG TGACCCGAGT CCTCGACGAC 
GGAGAGCTGC TGGTCCAGCA GACTAAAAAC AGTGACCGCA CACCGCTGGT TAGCGTCCTT 
CTGGAAGGCC CTCCTCACAG CGGGAAGACT GCCCTCGCTG CAAAGATTGC CGAGGAATCA 
AACTTCCCCT TCATCAAGAT CTGCTCCCCC GATAAGATGA TTGGGTTCTC TGAGACAGCC 
AAGTGTCAGG CCATGAAGAA GATCTTTGAT GATGCTTACA AATCTCAGCT CAGTTGTGTG 
GTCGTGGATG ACATTGAGAG GTTGCTCGAT TACGTCCCCA TCGGCCCCCG GTTCTCCAAC 
CTGGTGTTAC AAGCTCTGCT CGTGTTACTG AAAAAGGCAC CTCCTCAGGG CCGCAAGCTT 
CTCATCATCG GGACCACCAG CCGCAAAGAT GTCCTGCAGG AAATGGAGAT GCTCAACGCC 
TTCAGCACCA CCATCCACGT GCCCAACATC GCCACAGGAG AGCAGCTGCT GGAAGCTCTG 
GAGCTTTTGG GCAACTTCAA AGATAAGGAA CGCACCACAA TTGCTCAGCA AGTCAAAGGG 
AAGAAAGTCT GGATAGGAAT CAAGAAGTTA CTAATGTTGA TTGAGATGTC CCTGCAGATG 
GACCCTGAGT ACCGTGTGAG GAAATTCTTG GCCCTCATGA GAGAAGAAGG CGCTAGTCCC 
CTGGACTTTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interactions between N-Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus.
Hippocampus26(7)848-56(2016 Jul)
Xiong H,Cass? F,Zhou M,Xiong ZQ,Joels M,Martin S,Krugers HJ

Increased N-Ethylmaleimide-Sensitive Factor Expression in Amygdala and Perirhinal Cortex during Habituation of Taste Neophobia.
Neural plasticity20162726745(2016)
G?mez-Chac?n B,G?miz F,Foster TC,Gallo M

Identification of differentially expressed proteins and validation of the changes of N-ethylmaleimide-sensitive factor in rats with focal cerebral ischemia after transection of the cervical sympathetic trunk.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban34(6)801-7(2014 Dec)
Qu Y,Ma KN,Li XZ

The maintenance of long-term memory in the hippocampus depends on the interaction between N-ethylmaleimide-sensitive factor and GluA2.
Hippocampus24(9)1112-9(2014 Sep)
Migues PV,Hardt O,Finnie P,Wang YW,Nader K

Interactions between N-ethylmaleimide-sensitive factor and GluR2 in the nucleus accumbens contribute to the expression of locomotor sensitization to cocaine.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(10)3493-508(2014 Mar)
Lu HF,Wu PF,Yang YJ,Xiao W,Fan J,Liu J,Li YL,Luo Y,Hu ZL,Jin Y,Wang F,Chen JG

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Nsf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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