Tgm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tgm1
Entrez Gene ID	60335
Full Name	transglutaminase 1
General protein information	Preferred Names transglutaminase 1 Names protein-glutamine gamma-glutamyltransferase K TG(K) TGK TGase K TGase-1 epidermal TGase glutamine gamma-glutamyltransferase K transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase) transglutaminase 1, K polypeptide transglutaminase K
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	transglutaminase enzyme that catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins; stabilizes protein assemblies [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599792.1	XP_017455281.1	protein-glutamine gamma-glutamyltransferase K isoform X1
XM_006252014.3	XP_006252076.1	protein-glutamine gamma-glutamyltransferase K isoform X2
XM_008770691.2	XP_008768913.1	protein-glutamine gamma-glutamyltransferase K isoform X2
NM_031659.1	NP_113847.1	protein-glutamine gamma-glutamyltransferase K

Pathways from BioSystems

Homologies

	TGM1	XP_001113577.1
Canis lupus familiaris (dog)	TGM1	NP_001003079.1
Danio rerio (zebrafish)	LOC793448	XP_001331914.1
Danio rerio (zebrafish)	si:ch1073-138d10.2	XP_005169495.1
Drosophila melanogaster (fruit fly)	Tg	NP_609174.1
Mus musculus (house mouse)	Tgm1	NP_001155187.1
Danio rerio (zebrafish)	tgm1l4	NP_001025267.1
Danio rerio (zebrafish)	LOC100535918	XP_003201279.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009100	XP_319850.2
Homo sapiens (human)	TGM1	NP_000350.1
Pan troglodytes (chimpanzee)	TGM1	XP_001169496.3
Bos taurus (cattle)	TGM1	NP_001010991.1
Rattus norvegicus (Norway rat)	Tgm1	NP_113847.1
Danio rerio (zebrafish)	LOC100334173	XP_002665624.2
Danio rerio (zebrafish)	tgm1l1	XP_694950.3
Danio rerio (zebrafish)	tgm1l2	XP_001332075.5
Xenopus tropicalis (tropical clawed frog)	tgm1	XP_002939073.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and functional characterization of a novel transglutaminase 1 gene mutation associated with autosomal recessive congenital ichthyosis.
Zhang SQ, Li CX, Gao XQ, Qiu WY, Chen Q, Li XM, Zhou X, Tian X, Tang ZP, Zhao T, Zhang F, Zhang XB
International journal of dermatology55(2)201-7(2016 Feb)

Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls.
Petersen-Jones HG, Johnson KB, Hitomi K, Tykocki NR, Thompson JM, Watts SW
American journal of physiology. Heart and circulatory physiology308(6)H592-602(2015 Mar)

Transglutaminase inhibition protects against oxidative stress-induced neuronal death downstream of pathological ERK activation.
Basso M, Berlin J, Xia L, Sleiman SF, Ko B, Haskew-Layton R, Kim E, Antonyak MA, Cerione RA, Iismaa SE, Willis D, Cho S, Ratan RR
The Journal of neuroscience : the official journal of the Society for Neuroscience32(19)6561-9(2012 May)

Hornerin is a component of the epidermal cornified cell envelopes.
Henry J, Hsu CY, Haftek M, Nachat R, de Koning HD, Gardinal-Galera I, Hitomi K, Balica S, Jean-Decoster C, Schmitt AM, Paul C, Serre G, Simon M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(5)1567-76(2011 May)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression, activity, and functionality of TG1 and TG2 are decreased in the aorta, but not in the vena cava, from hypertensive rats.
Title: Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls.

Forced expression of TG1 or TG2 proteins is sufficient to induce neuronal death in Rattus norvegicus cortical neurons in vitro.
Title: Transglutaminase inhibition protects against oxidative stress-induced neuronal death downstream of pathological ERK activation.

Transglutaminase inhibition reduces fibrosis and preserves function in experimental chronic kidney disease
Title: Transglutaminase inhibition reduces fibrosis and preserves function in experimental chronic kidney disease.

These molecular and cellular data strongly suggest that TG(R) promotes axonal elongation at the surface of injured RGCs after optic nerve injury.
Title: Upregulation of retinal transglutaminase during the axonal elongation stage of goldfish optic nerve regeneration.

Cystamine and cysteamine increase brain levels of BDNF in Huntington disease via HSJ1b and transglutaminase
Title: Cystamine and cysteamine increase brain levels of BDNF in Huntington disease via HSJ1b and transglutaminase.

The following Tgm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tgm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08406	NM_031659.1 Latest version!	Rattus norvegicus transglutaminase 1 (Tgm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa08406	XM_008770691.2 Latest version!	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa08406	XM_006252014.3 Latest version!	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa58643	XM_017599792.1 Latest version!	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tgm1 ( NM_031659.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113847.1 Rattus norvegicus transglutaminase 1 (Tgm1), mRNA.

Price & Availability↑

CloneID	ORa08406
Clone ID Related Accession (Same CDS sequence)	XM_006252014.3 , XM_008770691.2 , NM_031659.1
Accession Version	NM_031659.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	protein-glutamine gamma-glutamyltransferase K
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M57263.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M57263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113847.1
CDS	104..2578
Translation	MEGPRSDVGRWGRSPWQPTTPSPEPEPEPEPDRSSRSRRGGGRSFWARCCGCCSCGNRADDDWGPEPSGSRSRGTSSRGGGSRGGDSRGRDSRGGRRPESRGSGVNAAGDGTIREGMLVVNGVDLLCSRSDQNRREHHTDEFEYDELILRRGQPFHIILFLNREYESSDRIALELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVTKTNGHNLTLRVHTSPNAIIGKFQFTVRTRSEAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVSVSRVVSAMVNSLDDNGVLIGNWTGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAINSNMREDITHIYKHPEGSEAERKAVEKAAAHGSKPNVYATRDSAEDVAMQVEAQDAVMGQDLTVSVVLTNRGSSRRTVKLHLYLCVTYYTGVSGPTFKETKKEVVLAPGASDTVAMPVAYKEYKPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPITLTNVVFRLEGSGLQRPKVLNVGDIGGNETVTLRQTFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPSSGGRGFSEAVGDSRSGENIPMAFRGGA

Target ORF information:

RefSeq Version	NM_031659.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transglutaminase 1 (Tgm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031659.1

ORF Insert Sequence:

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tgm1 ( XM_006252014.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252076.1 Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa08406
Clone ID Related Accession (Same CDS sequence)	XM_006252014.3 , XM_008770691.2 , NM_031659.1
Accession Version	XM_006252014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-glutamine gamma-glutamyltransferase K isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252076.1
CDS	1645..4119
Translation	MEGPRSDVGRWGRSPWQPTTPSPEPEPEPEPDRSSRSRRGGGRSFWARCCGCCSCGNRADDDWGPEPSGSRSRGTSSRGGGSRGGDSRGRDSRGGRRPESRGSGVNAAGDGTIREGMLVVNGVDLLCSRSDQNRREHHTDEFEYDELILRRGQPFHIILFLNREYESSDRIALELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVTKTNGHNLTLRVHTSPNAIIGKFQFTVRTRSEAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVSVSRVVSAMVNSLDDNGVLIGNWTGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAINSNMREDITHIYKHPEGSEAERKAVEKAAAHGSKPNVYATRDSAEDVAMQVEAQDAVMGQDLTVSVVLTNRGSSRRTVKLHLYLCVTYYTGVSGPTFKETKKEVVLAPGASDTVAMPVAYKEYKPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPITLTNVVFRLEGSGLQRPKVLNVGDIGGNETVTLRQTFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPSSGGRGFSEAVGDSRSGENIPMAFRGGA

Target ORF information:

RefSeq Version	XM_006252014.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252014.3

ORF Insert Sequence:

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tgm1 ( XM_008770691.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768913.1 Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa08406
Clone ID Related Accession (Same CDS sequence)	XM_006252014.3 , XM_008770691.2 , NM_031659.1
Accession Version	XM_008770691.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-glutamine gamma-glutamyltransferase K isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768913.1
CDS	1736..4210
Translation	MEGPRSDVGRWGRSPWQPTTPSPEPEPEPEPDRSSRSRRGGGRSFWARCCGCCSCGNRADDDWGPEPSGSRSRGTSSRGGGSRGGDSRGRDSRGGRRPESRGSGVNAAGDGTIREGMLVVNGVDLLCSRSDQNRREHHTDEFEYDELILRRGQPFHIILFLNREYESSDRIALELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVTKTNGHNLTLRVHTSPNAIIGKFQFTVRTRSEAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVSVSRVVSAMVNSLDDNGVLIGNWTGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAINSNMREDITHIYKHPEGSEAERKAVEKAAAHGSKPNVYATRDSAEDVAMQVEAQDAVMGQDLTVSVVLTNRGSSRRTVKLHLYLCVTYYTGVSGPTFKETKKEVVLAPGASDTVAMPVAYKEYKPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPITLTNVVFRLEGSGLQRPKVLNVGDIGGNETVTLRQTFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPSSGGRGFSEAVGDSRSGENIPMAFRGGA

Target ORF information:

RefSeq Version	XM_008770691.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770691.2

ORF Insert Sequence:

ATGGAAGGTC CTCGCTCAGA CGTGGGCCGC TGGGGTAGGA GCCCCTGGCA GCCCACGACA 
CCGTCGCCAG AGCCAGAGCC AGAGCCAGAG CCAGACAGAA GCTCGCGCTC CCGCCGAGGA 
GGAGGCCGCT CCTTCTGGGC TCGCTGTTGT GGCTGCTGCT CCTGCGGGAA CAGAGCTGAT 
GATGACTGGG GACCCGAACC TTCTGGCTCC AGAAGCCGAG GGACCAGCTC CCGGGGTGGA 
GGCTCCCGGG GTGGGGACTC TCGGGGTAGG GACTCTCGAG GTGGCCGAAG ACCTGAGTCT 
CGGGGCAGTG GTGTGAATGC AGCTGGAGAT GGCACCATCC GAGAGGGAAT GCTGGTTGTG 
AATGGTGTAG ATCTGCTGTG CTCGCGATCA GACCAGAACC GCCGAGAGCA CCACACCGAT 
GAGTTTGAAT ATGACGAGCT AATTTTGCGC CGTGGGCAGC CCTTCCACAT AATCCTCTTC 
CTGAACCGGG AGTATGAGTC CTCTGATCGC ATTGCCCTTG AGCTTCTCAT CGGAAACAAT 
CCTGAGGTGG GCAAGGGCAC CCACGTGATC ATCCCAGTGG GTAAGGGAGG CAGCGGTGGC 
TGGAAGGCCC AAGTGACTAA GACCAATGGA CACAACCTAA CCCTGCGCGT CCACACCTCC 
CCTAATGCCA TCATTGGCAA GTTTCAATTC ACTGTCCGTA CACGCTCAGA GGCTGGCGAG 
TTCCAGCTGC CCTTTGACCC CCGCAATGAG ATCTACATCC TCTTCAATCC CTGGTGTCCA 
GAGGACATAG TGTATGTGGA CCACGAAGAC TGGCGACAAG AATATGTGCT TAATGAGTCT 
GGAAGAATCT ACTATGGGAC AGAAGCACAG ATTGGCGAAC GGACCTGGAA TTATGGCCAG 
TTTGACCATG GGGTGCTGGA TGCCTGCCTG TACATTCTGG ATCGGAGGGG GATGCCATAT 
GGAGGTCGCG GGGACCCAGT CAGTGTCTCT CGGGTCGTCT CTGCCATGGT GAACTCCCTG 
GATGACAATG GAGTTCTGAT TGGGAACTGG ACTGGCGACT ACTCTCGAGG CACCAATCCC 
TCAGCGTGGG TGGGCAGTGT GGAGATCCTG CTTAGCTACC TACGCACCGG CTATTCCGTC 
CCCTATGGCC AATGCTGGGT CTTTGCCGGT GTGACCACCA CAGTGCTCCG ATGTCTGGGC 
CTTGCTACCC GTACTGTCAC CAACTTCAAC TCTGCACACG ACACGGACAC GTCCCTCACT 
ATGGACATTT ATTTTGATGA GAACATGAAG CCACTGGAGC ACCTGAACCA CGATTCTGTT 
TGGAACTTCC ACGTGTGGAA CGACTGCTGG ATGAAGAGGC CAGATCTGCC CTCAGGCTTT 
GATGGGTGGC AGGTTGTGGA TGCCACACCC CAGGAGACCA GCAGTGGCAT CTTCTGCTGT 
GGCCCCTGTT CAGTGGAGTC CATCAAGAAT GGCTTAGTCT ACATGAAGTA TGACACACCT 
TTCATTTTTG CCGAGGTAAA CAGTGATAAG GTATACTGGC AGCGGCAGGA TGACGGCAGC 
TTCAAGATCG TGTATGTGGA AGAGAAAGCC ATTGGCACAC TGATTGTCAC AAAGGCGATC 
AACTCCAACA TGCGAGAGGA CATCACCCAC ATCTATAAGC ACCCAGAAGG CTCAGAAGCA 
GAGAGGAAGG CTGTGGAAAA GGCTGCGGCC CATGGCAGCA AACCTAATGT GTATGCCACC 
CGGGACTCTG CTGAGGATGT GGCAATGCAG GTGGAGGCAC AGGATGCTGT GATGGGGCAG 
GATCTGACTG TCTCTGTGGT GTTGACCAAT CGTGGCAGTA GCCGACGCAC TGTGAAGTTG 
CACCTCTACC TTTGTGTCAC CTACTACACT GGTGTCTCTG GGCCTACCTT CAAGGAGACC 
AAGAAAGAAG TGGTATTAGC CCCAGGAGCC TCGGACACTG TGGCCATGCC TGTGGCCTAC 
AAGGAATACA AGCCCCACCT TGTGGACCAG GGGGCAATGT TGCTCAATGT CTCAGGCCAT 
GTCAAGGAGA GTGGGCAGGT ACTAGCCAAG CAACACACCT TCCGTTTGCG CACCCCAGAC 
CTCTCTCTGA CATTACTGGG AGCTGCAGTA GTTGGCCAGG AATGTGAAGT CCAGATCGTG 
TTCAAGAACC CCCTGCCTAT CACCCTCACC AACGTTGTCT TCCGGCTCGA AGGTTCTGGG 
TTACAGAGAC CCAAGGTCCT CAATGTTGGG GACATCGGGG GTAACGAGAC GGTTACACTG 
CGCCAGACAT TTGTTCCTGT GCGACCAGGC CCCCGCCAGC TCATTGCCAG TCTGGACAGT 
CCACAGCTTT CCCAAGTACA CGGTGTCATT CAAGTGGATG TGGCCCCATC CTCTGGAGGC 
AGAGGTTTCT CAGAGGCTGT AGGTGACAGT CGCTCCGGGG AGAACATACC TATGGCATTT 
CGAGGTGGAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tgm1 ( XM_017599792.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455281.1 Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58643
Clone ID Related Accession (Same CDS sequence)	XM_017599792.1
Accession Version	XM_017599792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-glutamine gamma-glutamyltransferase K isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCACCC TCTGTCTCTT CTACACAGGC ACAATGGAAG GTCCTCGCTC AGACGTGGGC 
CGCTGGGGTA GGAGCCCCTG GCAGCCCACG ACACCGTCGC CAGAGCCAGA GCCAGAGCCA 
GAGCCAGACA GAAGCTCGCG CTCCCGCCGA GGAGGAGGCC GCTCCTTCTG GGCTCGCTGT 
TGTGGCTGCT GCTCCTGCGG GAACAGAGCT GATGATGACT GGGGACCCGA ACCTTCTGGC 
TCCAGAAGCC GAGGGACCAG CTCCCGGGGT GGAGGCTCCC GGGGTGGGGA CTCTCGGGGT 
AGGGACTCTC GAGGTGGCCG AAGACCTGAG TCTCGGGGCA GTGGTGTGAA TGCAGCTGGA 
GATGGCACCA TCCGAGAGGG AATGCTGGTT GTGAATGGTG TAGATCTGCT GTGCTCGCGA 
TCAGACCAGA ACCGCCGAGA GCACCACACC GATGAGTTTG AATATGACGA GCTAATTTTG 
CGCCGTGGGC AGCCCTTCCA CATAATCCTC TTCCTGAACC GGGAGTATGA GTCCTCTGAT 
CGCATTGCCC TTGAGCTTCT CATCGGAAAC AATCCTGAGG TGGGCAAGGG CACCCACGTG 
ATCATCCCAG TGGGTAAGGG AGGCAGCGGT GGCTGGAAGG CCCAAGTGAC TAAGACCAAT 
GGACACAACC TAACCCTGCG CGTCCACACC TCCCCTAATG CCATCATTGG CAAGTTTCAA 
TTCACTGTCC GTACACGCTC AGAGGCTGGC GAGTTCCAGC TGCCCTTTGA CCCCCGCAAT 
GAGATCTACA TCCTCTTCAA TCCCTGGTGT CCAGAGGACA TAGTGTATGT GGACCACGAA 
GACTGGCGAC AAGAATATGT GCTTAATGAG TCTGGAAGAA TCTACTATGG GACAGAAGCA 
CAGATTGGCG AACGGACCTG GAATTATGGC CAGTTTGACC ATGGGGTGCT GGATGCCTGC 
CTGTACATTC TGGATCGGAG GGGGATGCCA TATGGAGGTC GCGGGGACCC AGTCAGTGTC 
TCTCGGGTCG TCTCTGCCAT GGTGAACTCC CTGGATGACA ATGGAGTTCT GATTGGGAAC 
TGGACTGGCG ACTACTCTCG AGGCACCAAT CCCTCAGCGT GGGTGGGCAG TGTGGAGATC 
CTGCTTAGCT ACCTACGCAC CGGCTATTCC GTCCCCTATG GCCAATGCTG GGTCTTTGCC 
GGTGTGACCA CCACAGTGCT CCGATGTCTG GGCCTTGCTA CCCGTACTGT CACCAACTTC 
AACTCTGCAC ACGACACGGA CACGTCCCTC ACTATGGACA TTTATTTTGA TGAGAACATG 
AAGCCACTGG AGCACCTGAA CCACGATTCT GTTTGGAACT TCCACGTGTG GAACGACTGC 
TGGATGAAGA GGCCAGATCT GCCCTCAGGC TTTGATGGGT GGCAGGTTGT GGATGCCACA 
CCCCAGGAGA CCAGCAGTGG CATCTTCTGC TGTGGCCCCT GTTCAGTGGA GTCCATCAAG 
AATGGCTTAG TCTACATGAA GTATGACACA CCTTTCATTT TTGCCGAGGT AAACAGTGAT 
AAGGTATACT GGCAGCGGCA GGATGACGGC AGCTTCAAGA TCGTGTATGT GGAAGAGAAA 
GCCATTGGCA CACTGATTGT CACAAAGGCG ATCAACTCCA ACATGCGAGA GGACATCACC 
CACATCTATA AGCACCCAGA AGGCTCAGAA GCAGAGAGGA AGGCTGTGGA AAAGGCTGCG 
GCCCATGGCA GCAAACCTAA TGTGTATGCC ACCCGGGACT CTGCTGAGGA TGTGGCAATG 
CAGGTGGAGG CACAGGATGC TGTGATGGGG CAGGATCTGA CTGTCTCTGT GGTGTTGACC 
AATCGTGGCA GTAGCCGACG CACTGTGAAG TTGCACCTCT ACCTTTGTGT CACCTACTAC 
ACTGGTGTCT CTGGGCCTAC CTTCAAGGAG ACCAAGAAAG AAGTGGTATT AGCCCCAGGA 
GCCTCGGACA CTGTGGCCAT GCCTGTGGCC TACAAGGAAT ACAAGCCCCA CCTTGTGGAC 
CAGGGGGCAA TGTTGCTCAA TGTCTCAGGC CATGTCAAGG AGAGTGGGCA GGTACTAGCC 
AAGCAACACA CCTTCCGTTT GCGCACCCCA GACCTCTCTC TGACATTACT GGGAGCTGCA 
GTAGTTGGCC AGGAATGTGA AGTCCAGATC GTGTTCAAGA ACCCCCTGCC TATCACCCTC 
ACCAACGTTG TCTTCCGGCT CGAAGGTTCT GGGTTACAGA GACCCAAGGT CCTCAATGTT 
GGGGACATCG GGGGTAACGA GACGGTTACA CTGCGCCAGA CATTTGTTCC TGTGCGACCA 
GGCCCCCGCC AGCTCATTGC CAGTCTGGAC AGTCCACAGC TTTCCCAAGT ACACGGTGTC 
ATTCAAGTGG ATGTGGCCCC ATCCTCTGGA GGCAGAGGTT TCTCAGAGGC TGTAGGTGAC 
AGTCGCTCCG GGGAGAACAT ACCTATGGCA TTTCGAGGTG GAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455281.1
CDS	379..2886
Translation	MSTLCLFYTGTMEGPRSDVGRWGRSPWQPTTPSPEPEPEPEPDRSSRSRRGGGRSFWARCCGCCSCGNRADDDWGPEPSGSRSRGTSSRGGGSRGGDSRGRDSRGGRRPESRGSGVNAAGDGTIREGMLVVNGVDLLCSRSDQNRREHHTDEFEYDELILRRGQPFHIILFLNREYESSDRIALELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVTKTNGHNLTLRVHTSPNAIIGKFQFTVRTRSEAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVSVSRVVSAMVNSLDDNGVLIGNWTGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAINSNMREDITHIYKHPEGSEAERKAVEKAAAHGSKPNVYATRDSAEDVAMQVEAQDAVMGQDLTVSVVLTNRGSSRRTVKLHLYLCVTYYTGVSGPTFKETKKEVVLAPGASDTVAMPVAYKEYKPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPITLTNVVFRLEGSGLQRPKVLNVGDIGGNETVTLRQTFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPSSGGRGFSEAVGDSRSGENIPMAFRGGA

Target ORF information:

RefSeq Version	XM_017599792.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transglutaminase 1 (Tgm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599792.1

ORF Insert Sequence:

ATGAGCACCC TCTGTCTCTT CTACACAGGC ACAATGGAAG GTCCTCGCTC AGACGTGGGC 
CGCTGGGGTA GGAGCCCCTG GCAGCCCACG ACACCGTCGC CAGAGCCAGA GCCAGAGCCA 
GAGCCAGACA GAAGCTCGCG CTCCCGCCGA GGAGGAGGCC GCTCCTTCTG GGCTCGCTGT 
TGTGGCTGCT GCTCCTGCGG GAACAGAGCT GATGATGACT GGGGACCCGA ACCTTCTGGC 
TCCAGAAGCC GAGGGACCAG CTCCCGGGGT GGAGGCTCCC GGGGTGGGGA CTCTCGGGGT 
AGGGACTCTC GAGGTGGCCG AAGACCTGAG TCTCGGGGCA GTGGTGTGAA TGCAGCTGGA 
GATGGCACCA TCCGAGAGGG AATGCTGGTT GTGAATGGTG TAGATCTGCT GTGCTCGCGA 
TCAGACCAGA ACCGCCGAGA GCACCACACC GATGAGTTTG AATATGACGA GCTAATTTTG 
CGCCGTGGGC AGCCCTTCCA CATAATCCTC TTCCTGAACC GGGAGTATGA GTCCTCTGAT 
CGCATTGCCC TTGAGCTTCT CATCGGAAAC AATCCTGAGG TGGGCAAGGG CACCCACGTG 
ATCATCCCAG TGGGTAAGGG AGGCAGCGGT GGCTGGAAGG CCCAAGTGAC TAAGACCAAT 
GGACACAACC TAACCCTGCG CGTCCACACC TCCCCTAATG CCATCATTGG CAAGTTTCAA 
TTCACTGTCC GTACACGCTC AGAGGCTGGC GAGTTCCAGC TGCCCTTTGA CCCCCGCAAT 
GAGATCTACA TCCTCTTCAA TCCCTGGTGT CCAGAGGACA TAGTGTATGT GGACCACGAA 
GACTGGCGAC AAGAATATGT GCTTAATGAG TCTGGAAGAA TCTACTATGG GACAGAAGCA 
CAGATTGGCG AACGGACCTG GAATTATGGC CAGTTTGACC ATGGGGTGCT GGATGCCTGC 
CTGTACATTC TGGATCGGAG GGGGATGCCA TATGGAGGTC GCGGGGACCC AGTCAGTGTC 
TCTCGGGTCG TCTCTGCCAT GGTGAACTCC CTGGATGACA ATGGAGTTCT GATTGGGAAC 
TGGACTGGCG ACTACTCTCG AGGCACCAAT CCCTCAGCGT GGGTGGGCAG TGTGGAGATC 
CTGCTTAGCT ACCTACGCAC CGGCTATTCC GTCCCCTATG GCCAATGCTG GGTCTTTGCC 
GGTGTGACCA CCACAGTGCT CCGATGTCTG GGCCTTGCTA CCCGTACTGT CACCAACTTC 
AACTCTGCAC ACGACACGGA CACGTCCCTC ACTATGGACA TTTATTTTGA TGAGAACATG 
AAGCCACTGG AGCACCTGAA CCACGATTCT GTTTGGAACT TCCACGTGTG GAACGACTGC 
TGGATGAAGA GGCCAGATCT GCCCTCAGGC TTTGATGGGT GGCAGGTTGT GGATGCCACA 
CCCCAGGAGA CCAGCAGTGG CATCTTCTGC TGTGGCCCCT GTTCAGTGGA GTCCATCAAG 
AATGGCTTAG TCTACATGAA GTATGACACA CCTTTCATTT TTGCCGAGGT AAACAGTGAT 
AAGGTATACT GGCAGCGGCA GGATGACGGC AGCTTCAAGA TCGTGTATGT GGAAGAGAAA 
GCCATTGGCA CACTGATTGT CACAAAGGCG ATCAACTCCA ACATGCGAGA GGACATCACC 
CACATCTATA AGCACCCAGA AGGCTCAGAA GCAGAGAGGA AGGCTGTGGA AAAGGCTGCG 
GCCCATGGCA GCAAACCTAA TGTGTATGCC ACCCGGGACT CTGCTGAGGA TGTGGCAATG 
CAGGTGGAGG CACAGGATGC TGTGATGGGG CAGGATCTGA CTGTCTCTGT GGTGTTGACC 
AATCGTGGCA GTAGCCGACG CACTGTGAAG TTGCACCTCT ACCTTTGTGT CACCTACTAC 
ACTGGTGTCT CTGGGCCTAC CTTCAAGGAG ACCAAGAAAG AAGTGGTATT AGCCCCAGGA 
GCCTCGGACA CTGTGGCCAT GCCTGTGGCC TACAAGGAAT ACAAGCCCCA CCTTGTGGAC 
CAGGGGGCAA TGTTGCTCAA TGTCTCAGGC CATGTCAAGG AGAGTGGGCA GGTACTAGCC 
AAGCAACACA CCTTCCGTTT GCGCACCCCA GACCTCTCTC TGACATTACT GGGAGCTGCA 
GTAGTTGGCC AGGAATGTGA AGTCCAGATC GTGTTCAAGA ACCCCCTGCC TATCACCCTC 
ACCAACGTTG TCTTCCGGCT CGAAGGTTCT GGGTTACAGA GACCCAAGGT CCTCAATGTT 
GGGGACATCG GGGGTAACGA GACGGTTACA CTGCGCCAGA CATTTGTTCC TGTGCGACCA 
GGCCCCCGCC AGCTCATTGC CAGTCTGGAC AGTCCACAGC TTTCCCAAGT ACACGGTGTC 
ATTCAAGTGG ATGTGGCCCC ATCCTCTGGA GGCAGAGGTT TCTCAGAGGC TGTAGGTGAC 
AGTCGCTCCG GGGAGAACAT ACCTATGGCA TTTCGAGGTG GAGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and functional characterization of a novel transglutaminase 1 gene mutation associated with autosomal recessive congenital ichthyosis.
International journal of dermatology55(2)201-7(2016 Feb)
Zhang SQ,Li CX,Gao XQ,Qiu WY,Chen Q,Li XM,Zhou X,Tian X,Tang ZP,Zhao T,Zhang F,Zhang XB

Transglutaminase activity is decreased in large arteries from hypertensive rats compared with normotensive controls.
American journal of physiology. Heart and circulatory physiology308(6)H592-602(2015 Mar)
Petersen-Jones HG,Johnson KB,Hitomi K,Tykocki NR,Thompson JM,Watts SW

Transglutaminase inhibition protects against oxidative stress-induced neuronal death downstream of pathological ERK activation.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(19)6561-9(2012 May)
Basso M,Berlin J,Xia L,Sleiman SF,Ko B,Haskew-Layton R,Kim E,Antonyak MA,Cerione RA,Iismaa SE,Willis D,Cho S,Ratan RR

Hornerin is a component of the epidermal cornified cell envelopes.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology25(5)1567-76(2011 May)
Henry J,Hsu CY,Haftek M,Nachat R,de Koning HD,Gardinal-Galera I,Hitomi K,Balica S,Jean-Decoster C,Schmitt AM,Paul C,Serre G,Simon M

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Journal of proteome research8(3)1304-14(2009 Mar)
Gonzalez-Begne M,Lu B,Han X,Hagen FK,Hand AR,Melvin JE,Yates JR

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Tgm1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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