Rapgef3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rapgef3
Entrez Gene ID	59326
Full Name	Rap guanine nucleotide exchange factor 3
Synonyms	Epac
General protein information	Preferred Names Rap guanine nucleotide exchange factor 3 Names rap guanine nucleotide exchange factor 3 Rap guanine nucleotide exchange factor (GEF) 3 cAMP-GEFI cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI) epac 1 exchange factor directly activated by cAMP 1 exchange protein directly activated by cAMP 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	binds cAMP and activates the Ras superfamily guanine nucleotide binding protein (Rap1A)in a PKA-independent manner [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765778.2	XP_008764000.1	rap guanine nucleotide exchange factor 3 isoform X1
XM_008765779.1	XP_008764001.1	rap guanine nucleotide exchange factor 3 isoform X3
XM_006242352.3	XP_006242414.1	rap guanine nucleotide exchange factor 3 isoform X2
NM_021690.1	NP_067722.1	rap guanine nucleotide exchange factor 3
XM_008765780.2	XP_008764002.1	rap guanine nucleotide exchange factor 3 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAPGEF3	XP_001162479.2
Xenopus tropicalis (tropical clawed frog)	rapgef3	XP_002935702.2
Rattus norvegicus (Norway rat)	Rapgef3	NP_067722.1
Danio rerio (zebrafish)	LOC101886094	XP_005162410.1
	RAPGEF3	XP_001098291.2
Gallus gallus (chicken)	RAPGEF3	XP_424494.4
Bos taurus (cattle)	RAPGEF3	NP_001073771.1
Homo sapiens (human)	RAPGEF3	NP_001092001.1
Canis lupus familiaris (dog)	RAPGEF3	NP_001093414.1
Mus musculus (house mouse)	Rapgef3	NP_001171281.1

Gene Ontology
Bibliography

Related articles in PubMed

Early effects of Epac depend on the fine-tuning of the sarcoplasmic reticulum Ca
Lezcano N, Mari?ngelo JIE, Vittone L, Wehrens XHT, Said M, Mundi?a-Weilenmann C
Journal of molecular and cellular cardiology1141-9(2017 Oct)

Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.
Sun W, Jiao W, Huang Y, Li R, Zhang Z, Wang J, Lei T
Biochemical and biophysical research communications485(2)355-359(2017 04)

Epac-Rap1-activated mesenchymal stem cells improve cardiac function in rat model of myocardial infarction.
Khan I, Ali A, Akhter MA, Naeem N, Chotani MA, Iqbal H, Kabir N, Atiq M, Salim A
Cardiovascular therapeutics35(2)(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rap guanine nucleotide exchange factors Epac1 and Epac2 induces the phosphorylation of extracellular signal-regulated kinases ERK1/2 in pituitary tumor GH3 cells.
Title: Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.

Pharmacological activation of Epac-Rap1 improves cardiac function through better survival, adhesion and differentiation of transplanted cells. Transplantation of these MSCs in the infarct area restored functional myocardium.
Title: Epac-Rap1-activated mesenchymal stem cells improve cardiac function in rat model of myocardial infarction.

Transactivation of protein kinase C-[epsilon] during inflammatory neuropathic pain, and nociceptor priming is mediated by the cAMP sensor exchange protein directly activated by cAMP (EPAC).
Title: Acquired Exchange Protein Directly Activated by Cyclic Adenosine Monophosphate Activity Induced by p38 Mitogen-activated Protein Kinase in Primary Afferent Neurons Contributes to Sustaining Postincisional Nociception.

EPAC-dependent vasorelaxations occur in part via activation of Kv7 channels. This contributes to the isoproterenol-mediated relaxation in mesenteric, but not renal, arteries.
Title: Kv7 Channel Activation Underpins EPAC-Dependent Relaxations of Rat Arteries.

after inflammation, an Epac-PKC dependent increase in F-actin in dorsal root ganglion neurons enhances the membrane expression of P2X3Rs to bring about sensitization of P2X3Rs and abnormal pain behaviors.
Title: EXPRESS: F-actin links Epac-PKC signaling to purinergic P2X3 receptors sensitization in dorsal root ganglia following inflammation.

The following Rapgef3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05070	NM_021690.1 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa30746	XM_006242352.3 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa30747	XM_008765779.1 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa43501	XM_008765778.2 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa43502	XM_008765780.2 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rapgef3 ( NM_021690.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067722.1 Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), mRNA.

Price & Availability↑

CloneID	ORa05070
Clone ID Related Accession (Same CDS sequence)	NM_021690.1
Accession Version	NM_021690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U78167.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U78167.1, BC107667.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCTGA AGAGAATGCA CCGTCCCCGG TGCTGCTCTT ACCAGCTAGT GTTCGAGCAC 
CGGCGCCCAA GCTGCATCCA GGGACTTCGC TGGACGCCAC TTACCAACAG TGAGGGCTCC 
CTGGACTTCA GAGTGAGCCT GGAGCAGGCC ACCACAGAGC ATGTGCACAA GGCCGGGAAG 
CTCCTGTACC GTCATCTCTT GGCAACGTAC CCTACCCTCA TCCGAGACAG AAAATACCAT 
CTGCGACTAC ATCGGCAGTG CTGCTCTGGC CGGGAGCTAG TGGATGGGAT CTTGGCTCTG 
GGTCTTGGGG TCCACTCACG GAGCCAAGCT GTGGGCATCT GCCAGGTGTT GCTGGATGAG 
GGTGCCCTTT GCCATGTAAA ACATGACTGG ACCTTCCAGG ACCGAGACGC CCAATTCTAC 
AGATTCCCTG GACCGGAGCC CCAGCCTGCA GGAACTCATG ACGTGGAAGA GGAGCTTGTT 
GAGGCAATGG CCCTACTCTC CCAGCGAGGG CCTGATGCCC TACTCACTGT TGCACTCCGG 
AAGTCCCCGG GTCAGCGTAC AGATGAAGAG CTGGACCTCA TCTTCGAGGA GCTCGTACAT 
ATCAAGGCGG TGGCTCACCT TTCTAACTCG GTGAAACGGG AACTAGCTGC TGTTCTGCTC 
TTTGAACCAC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGTACCTCA 
TGGTACATTA TCTGGAAGGG ATCTGTCAAT GTGGTGACCC GTGGCAAGGG GCTGGTGACC 
ACGTTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGACGC ACCTCGAGCA 
GCCACCATCA TCCTTCGAGA AAATAACTGT CACTTTCTGC GTGTGGACAA GCAGGACTTC 
AACCGCATCA TCAAGGATGT GGAAGCAAAA ACCATGAGAC TGGAAGAACA CGGCAAAGTG 
GTGTTAGTTT TGGAGAGAAC CTCTCAGGGT GCTGGCCCTT CCCGCCCTCC GACCCCAGGC 
AGGAACCGAT ATACGGTAAT GTCTGGCACC CCAGAGAAAA TCCTAGAACT CCTGTTGGAG 
GCTATGAGAC CGGATTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTG 
CTGACGCACA GTGTCTTCAT GCCCTGCACA CAGCTCTTTG CCGCCCTCCT GCACCACTTC 
CACGTGGAGC CATCAGAGCC TGCCGGGGGC AGCGAGCAGG AACGCAGCAC CTACATCTGC 
AACAAGAGGC AGCAGATTCT GCGTCTGGTC AGCCGGTGGG TGGCCCTCTA CAGCCCCATG 
CTCCGCTCAG ATCCCGTGGC CACCAGCTTC CTCCAGAAAC TCTCAGACCT GGTGAGCAGA 
GATACCCGAC TTAGCAACTT GCTGAGGGAA CAGTATCCGG AGAGACGGCG ACACCACAGG 
TTGGAGAATG GCTGTGGGAA TGTATCTCCT CAGACCAAGG CCCGGAATGC ACCTGTTTGG 
TTTCCTAACC ATGAGGAACC CCTCCCAAGC AGCGCTGGGG CCATCCGAGT CGGGGACAAA 
GTCCCCTATG ATATCTGCAG ACCCGACCAC TCGGTGCTGA CCCTGCACCT GCCGGTGACA 
GCCTCCGTGA GGGAAGTGAT GGCAGCTTTG GCCCATGAGG ACCACTGGAC CAAGGGGCAG 
GTGCTGGTAA AGGTCAATTC TGCCGGTGAT GTCGTTGGCT TGCAGCCAGA TGCCCGCGGT 
GTGGCCACAT CCCTGGGGCT CAATGAGCGG ATCTTTGTTG TCGACCCACA GGAAGTGCAC 
GAGCTGACCC CACACCCTGA GCAGCTGGGG CCCACTCTGG GTTCTTCTGA GATGCTGGAC 
CTAGTGAGTG CCAAGGACCT GGCAGGCCAG CTCACAGAGC ATGACTGGAA CCTCTTCAAC 
AGGATCCACC AGGTGGAGCT GATCCACTAT GTACTGGGCC CCCAGCACCT GCGGGACGTC 
ACCACTGCAA ACCTGGAGCG CTTCATGCGA CGCTTCAACG AGCTGCAGTA CTGGGTGGCC 
ACCGAACTCT GTCTCTGCCC GGTTCCTGGC CCCCGGGCTC AGCTACTCCG GAAGTTCATC 
AAGCTGGCAG CCCACCTCAA GGAGCAGAAG AATCTCAACT CTTTCTTTGC GGTCATGTTT 
GGCCTCAGCA ACTCGGCCAT CAGCCGCCTG GCCCACACCT GGGAGCGTCT GCCCCATAAA 
GTACGGAAGC TGTACTCGGC CCTGGAAAGG TTGCTGGACC CTTCCTGGAA CCACCGAGTG 
TACCGATTGG CTCTCACCAA GCTCTCTCCT CCTGTCATCC CTTTCATGCC CCTGCTACTC 
AAAGACATGA CCTTCATTCA TGAAGGGAAC CACACACTGG TAGAAAACCT CATCAACTTT 
GAGAAGATGC GAATGATGGC CAGAGCCGTG CGGATGCTCC ACCACTGCCG AAGCCACAGC 
ACCGCGCCTC TATCACCACT CAGAAGCCGG GTTTCCCACA TCCACGAGGA CAGCCAGGCA 
TCAAGAATCT CCACATGTTC CGAGCAGTCC CTGAGCACCC GGAGTCCAGC CAGCACCTGG 
GCTTATGTCC AGCAGCTGAA GGTCATTGAC AACCAGCGGG AACTGTCCCG CCTCTCCCGG 
GAACTGGAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067722.1
CDS	197..2851
Translation	MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELVHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTRGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSPQTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQVLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	NM_021690.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021690.1

ORF Insert Sequence:

ATGGTGCTGA AGAGAATGCA CCGTCCCCGG TGCTGCTCTT ACCAGCTAGT GTTCGAGCAC 
CGGCGCCCAA GCTGCATCCA GGGACTTCGC TGGACGCCAC TTACCAACAG TGAGGGCTCC 
CTGGACTTCA GAGTGAGCCT GGAGCAGGCC ACCACAGAGC ATGTGCACAA GGCCGGGAAG 
CTCCTGTACC GTCATCTCTT GGCAACGTAC CCTACCCTCA TCCGAGACAG AAAATACCAT 
CTGCGACTAC ATCGGCAGTG CTGCTCTGGC CGGGAGCTAG TGGATGGGAT CTTGGCTCTG 
GGTCTTGGGG TCCACTCACG GAGCCAAGCT GTGGGCATCT GCCAGGTGTT GCTGGATGAG 
GGTGCCCTTT GCCATGTAAA ACATGACTGG ACCTTCCAGG ACCGAGACGC CCAATTCTAC 
AGATTCCCTG GACCGGAGCC CCAGCCTGCA GGAACTCATG ACGTGGAAGA GGAGCTTGTT 
GAGGCAATGG CCCTACTCTC CCAGCGAGGG CCTGATGCCC TACTCACTGT TGCACTCCGG 
AAGTCCCCGG GTCAGCGTAC AGATGAAGAG CTGGACCTCA TCTTCGAGGA GCTCGTACAT 
ATCAAGGCGG TGGCTCACCT TTCTAACTCG GTGAAACGGG AACTAGCTGC TGTTCTGCTC 
TTTGAACCAC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGTACCTCA 
TGGTACATTA TCTGGAAGGG ATCTGTCAAT GTGGTGACCC GTGGCAAGGG GCTGGTGACC 
ACGTTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGACGC ACCTCGAGCA 
GCCACCATCA TCCTTCGAGA AAATAACTGT CACTTTCTGC GTGTGGACAA GCAGGACTTC 
AACCGCATCA TCAAGGATGT GGAAGCAAAA ACCATGAGAC TGGAAGAACA CGGCAAAGTG 
GTGTTAGTTT TGGAGAGAAC CTCTCAGGGT GCTGGCCCTT CCCGCCCTCC GACCCCAGGC 
AGGAACCGAT ATACGGTAAT GTCTGGCACC CCAGAGAAAA TCCTAGAACT CCTGTTGGAG 
GCTATGAGAC CGGATTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTG 
CTGACGCACA GTGTCTTCAT GCCCTGCACA CAGCTCTTTG CCGCCCTCCT GCACCACTTC 
CACGTGGAGC CATCAGAGCC TGCCGGGGGC AGCGAGCAGG AACGCAGCAC CTACATCTGC 
AACAAGAGGC AGCAGATTCT GCGTCTGGTC AGCCGGTGGG TGGCCCTCTA CAGCCCCATG 
CTCCGCTCAG ATCCCGTGGC CACCAGCTTC CTCCAGAAAC TCTCAGACCT GGTGAGCAGA 
GATACCCGAC TTAGCAACTT GCTGAGGGAA CAGTATCCGG AGAGACGGCG ACACCACAGG 
TTGGAGAATG GCTGTGGGAA TGTATCTCCT CAGACCAAGG CCCGGAATGC ACCTGTTTGG 
TTTCCTAACC ATGAGGAACC CCTCCCAAGC AGCGCTGGGG CCATCCGAGT CGGGGACAAA 
GTCCCCTATG ATATCTGCAG ACCCGACCAC TCGGTGCTGA CCCTGCACCT GCCGGTGACA 
GCCTCCGTGA GGGAAGTGAT GGCAGCTTTG GCCCATGAGG ACCACTGGAC CAAGGGGCAG 
GTGCTGGTAA AGGTCAATTC TGCCGGTGAT GTCGTTGGCT TGCAGCCAGA TGCCCGCGGT 
GTGGCCACAT CCCTGGGGCT CAATGAGCGG ATCTTTGTTG TCGACCCACA GGAAGTGCAC 
GAGCTGACCC CACACCCTGA GCAGCTGGGG CCCACTCTGG GTTCTTCTGA GATGCTGGAC 
CTAGTGAGTG CCAAGGACCT GGCAGGCCAG CTCACAGAGC ATGACTGGAA CCTCTTCAAC 
AGGATCCACC AGGTGGAGCT GATCCACTAT GTACTGGGCC CCCAGCACCT GCGGGACGTC 
ACCACTGCAA ACCTGGAGCG CTTCATGCGA CGCTTCAACG AGCTGCAGTA CTGGGTGGCC 
ACCGAACTCT GTCTCTGCCC GGTTCCTGGC CCCCGGGCTC AGCTACTCCG GAAGTTCATC 
AAGCTGGCAG CCCACCTCAA GGAGCAGAAG AATCTCAACT CTTTCTTTGC GGTCATGTTT 
GGCCTCAGCA ACTCGGCCAT CAGCCGCCTG GCCCACACCT GGGAGCGTCT GCCCCATAAA 
GTACGGAAGC TGTACTCGGC CCTGGAAAGG TTGCTGGACC CTTCCTGGAA CCACCGAGTG 
TACCGATTGG CTCTCACCAA GCTCTCTCCT CCTGTCATCC CTTTCATGCC CCTGCTACTC 
AAAGACATGA CCTTCATTCA TGAAGGGAAC CACACACTGG TAGAAAACCT CATCAACTTT 
GAGAAGATGC GAATGATGGC CAGAGCCGTG CGGATGCTCC ACCACTGCCG AAGCCACAGC 
ACCGCGCCTC TATCACCACT CAGAAGCCGG GTTTCCCACA TCCACGAGGA CAGCCAGGCA 
TCAAGAATCT CCACATGTTC CGAGCAGTCC CTGAGCACCC GGAGTCCAGC CAGCACCTGG 
GCTTATGTCC AGCAGCTGAA GGTCATTGAC AACCAGCGGG AACTGTCCCG CCTCTCCCGG 
GAACTGGAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef3 ( XM_006242352.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242414.1 Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa30746
Clone ID Related Accession (Same CDS sequence)	XM_006242352.3
Accession Version	XM_006242352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTCA GCTGGCCAGG TGAGAACCAC TGGCAGGTGG GCCCAGCTGT GGTGGAGAGT 
CCAGCTGTGG GGGCACCGCA GGTGGGAGGT CTCCCGGACG TGGTTCCGGA GGGCACGCTG 
CTCAATATGG TGCTGAAGAG AATGCACCGT CCCCGGTGCT GCTCTTACCA GCTAGTGTTC 
GAGCACCGGC GCCCAAGCTG CATCCAGGGA CTTCGCTGGA CGCCACTTAC CAACAGTGAG 
GGCTCCCTGG ACTTCAGAGT GAGCCTGGAG CAGGCCACCA CAGAGCATGT GCACAAGGCC 
GGGAAGCTCC TGTACCGTCA TCTCTTGGCA ACGTACCCTA CCCTCATCCG AGACAGAAAA 
TACCATCTGC GACTACATCG GCAGTGCTGC TCTGGCCGGG AGCTAGTGGA TGGGATCTTG 
GCTCTGGGTC TTGGGGTCCA CTCACGGAGC CAAGCTGTGG GCATCTGCCA GGTGTTGCTG 
GATGAGGGTG CCCTTTGCCA TGTAAAACAT GACTGGACCT TCCAGGACCG AGACGCCCAA 
TTCTACAGAT TCCCTGGACC GGAGCCCCAG CCTGCAGGAA CTCATGACGT GGAAGAGGAG 
CTTGTTGAGG CAATGGCCCT ACTCTCCCAG CGAGGGCCTG ATGCCCTACT CACTGTTGCA 
CTCCGGAAGT CCCCGGGTCA GCGTACAGAT GAAGAGCTGG ACCTCATCTT CGAGGAGCTG 
CTACATATCA AGGCGGTGGC TCACCTTTCT AACTCGGTGA AACGGGAACT AGCTGCTGTT 
CTGCTCTTTG AACCACACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGT 
ACCTCATGGT ACATTATCTG GAAGGGATCT GTCAATGTGG TGACCCATGG CAAGGGGCTG 
GTGACCACGT TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGACGCACCT 
CGAGCAGCCA CCATCATCCT TCGAGAAAAT AACTGTCACT TTCTGCGTGT GGACAAGCAG 
GACTTCAACC GCATCATCAA GGATGTGGAA GCAAAAACCA TGAGACTGGA AGAACACGGC 
AAAGTGGTGT TAGTTTTGGA GAGAACCTCT CAGGGTGCTG GCCCTTCCCG CCCTCCGACC 
CCAGGCAGGA ACCGATATAC GGTAATGTCT GGCACCCCAG AGAAAATCCT AGAACTCCTG 
TTGGAGGCTA TGAGACCGGA TTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTGCTGA CGCACAGTGT CTTCATGCCC TGCACACAGC TCTTTGCCGC CCTCCTGCAC 
CACTTCCACG TGGAGCCATC AGAGCCTGCC GGGGGCAGCG AGCAGGAACG CAGCACCTAC 
ATCTGCAACA AGAGGCAGCA GATTCTGCGT CTGGTCAGCC GGTGGGTGGC CCTCTACAGC 
CCCATGCTCC GCTCAGATCC CGTGGCCACC AGCTTCCTCC AGAAACTCTC AGACCTGGTG 
AGCAGAGATA CCCGACTTAG CAACTTGCTG AGGGAACAGT ATCCGGAGAG ACGGCGACAC 
CACAGGTTGG AGAATGGCTG TGGGAATGTA TCTCCTCAGA CCAAGGCCCG GAATGCACCT 
GTTTGGTTTC CTAACCATGA GGAACCCCTC CCAAGCAGCG CTGGGGCCAT CCGAGTCGGG 
GACAAAGTCC CCTATGATAT CTGCAGACCC GACCACTCGG TGCTGACCCT GCACCTGCCG 
GTGACAGCCT CCGTGAGGGA AGTGATGGCA GCTTTGGCCC ATGAGGACCA CTGGACCAAG 
GGGCAGGTGC TGGTAAAGGT CAATTCTGCC GGTGATGTCG TTGGCTTGCA GCCAGATGCC 
CGCGGTGTGG CCACATCCCT GGGGCTCAAT GAGCGGATCT TTGTTGTCGA CCCACAGGAA 
GTGCACGAGC TGACCCCACA CCCTGAGCAG CTGGGGCCCA CTCTGGGTTC TTCTGAGATG 
CTGGACCTAG TGAGTGCCAA GGACCTGGCA GGCCAGCTCA CAGAGCATGA CTGGAACCTC 
TTCAACAGGA TCCACCAGGT GGAGCTGATC CACTATGTAC TGGGCCCCCA GCACCTGCGG 
GACGTCACCA CTGCAAACCT GGAGCGCTTC ATGCGACGCT TCAACGAGCT GCAGTACTGG 
GTGGCCACCG AACTCTGTCT CTGCCCGGTT CCTGGCCCCC GGGCTCAGCT ACTCCGGAAG 
TTCATCAAGC TGGCAGCCCA CCTCAAGGAG CAGAAGAATC TCAACTCTTT CTTTGCGGTC 
ATGTTTGGCC TCAGCAACTC GGCCATCAGC CGCCTGGCCC ACACCTGGGA GCGTCTGCCC 
CATAAAGTAC GGAAGCTGTA CTCGGCCCTG GAAAGGTTGC TGGACCCTTC CTGGAACCAC 
CGAGTGTACC GATTGGCTCT CACCAAGCTC TCTCCTCCTG TCATCCCTTT CATGCCCCTG 
CTACTCAAAG ACATGACCTT CATCCATGAA GGGAACCACA CACTGGTAGA AAACCTCATC 
AACTTTGAGA AGATGCGAAT GATGGCCAGA GCCGTGCGGA TGCTCCACCA CTGCCGAAGC 
CACAGCACCG CGCCTCTATC ACCACTCAGA AGCCGGGTTT CCCACATCCA CGAGGACAGC 
CAGGCATCAA GAATCTCCAC ATGTTCCGAG CAGTCCCTGA GCACCCGGAG TCCAGCCAGC 
ACCTGGGCTT ATGTCCAGCA GCTGAAGGTC ATTGACAACC AGCGGGAACT GTCCCGCCTC 
TCCCGGGAAC TGGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242414.1
CDS	118..2898
Translation	MKVSWPGENHWQVGPAVVESPAVGAPQVGGLPDVVPEGTLLNMVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSPQTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQVLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_006242352.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242352.3

ORF Insert Sequence:

ATGAAGGTCA GCTGGCCAGG TGAGAACCAC TGGCAGGTGG GCCCAGCTGT GGTGGAGAGT 
CCAGCTGTGG GGGCACCGCA GGTGGGAGGT CTCCCGGACG TGGTTCCGGA GGGCACGCTG 
CTCAATATGG TGCTGAAGAG AATGCACCGT CCCCGGTGCT GCTCTTACCA GCTAGTGTTC 
GAGCACCGGC GCCCAAGCTG CATCCAGGGA CTTCGCTGGA CGCCACTTAC CAACAGTGAG 
GGCTCCCTGG ACTTCAGAGT GAGCCTGGAG CAGGCCACCA CAGAGCATGT GCACAAGGCC 
GGGAAGCTCC TGTACCGTCA TCTCTTGGCA ACGTACCCTA CCCTCATCCG AGACAGAAAA 
TACCATCTGC GACTACATCG GCAGTGCTGC TCTGGCCGGG AGCTAGTGGA TGGGATCTTG 
GCTCTGGGTC TTGGGGTCCA CTCACGGAGC CAAGCTGTGG GCATCTGCCA GGTGTTGCTG 
GATGAGGGTG CCCTTTGCCA TGTAAAACAT GACTGGACCT TCCAGGACCG AGACGCCCAA 
TTCTACAGAT TCCCTGGACC GGAGCCCCAG CCTGCAGGAA CTCATGACGT GGAAGAGGAG 
CTTGTTGAGG CAATGGCCCT ACTCTCCCAG CGAGGGCCTG ATGCCCTACT CACTGTTGCA 
CTCCGGAAGT CCCCGGGTCA GCGTACAGAT GAAGAGCTGG ACCTCATCTT CGAGGAGCTG 
CTACATATCA AGGCGGTGGC TCACCTTTCT AACTCGGTGA AACGGGAACT AGCTGCTGTT 
CTGCTCTTTG AACCACACAG CAAGGCAGGA ACTGTGTTGT TCAGCCAGGG GGACAAGGGT 
ACCTCATGGT ACATTATCTG GAAGGGATCT GTCAATGTGG TGACCCATGG CAAGGGGCTG 
GTGACCACGT TGCACGAGGG AGATGACTTT GGACAGCTGG CTCTGGTGAA CGACGCACCT 
CGAGCAGCCA CCATCATCCT TCGAGAAAAT AACTGTCACT TTCTGCGTGT GGACAAGCAG 
GACTTCAACC GCATCATCAA GGATGTGGAA GCAAAAACCA TGAGACTGGA AGAACACGGC 
AAAGTGGTGT TAGTTTTGGA GAGAACCTCT CAGGGTGCTG GCCCTTCCCG CCCTCCGACC 
CCAGGCAGGA ACCGATATAC GGTAATGTCT GGCACCCCAG AGAAAATCCT AGAACTCCTG 
TTGGAGGCTA TGAGACCGGA TTCCAGTGCT CATGACCCAA CAGAGACATT CCTCAGTGAC 
TTCCTGCTGA CGCACAGTGT CTTCATGCCC TGCACACAGC TCTTTGCCGC CCTCCTGCAC 
CACTTCCACG TGGAGCCATC AGAGCCTGCC GGGGGCAGCG AGCAGGAACG CAGCACCTAC 
ATCTGCAACA AGAGGCAGCA GATTCTGCGT CTGGTCAGCC GGTGGGTGGC CCTCTACAGC 
CCCATGCTCC GCTCAGATCC CGTGGCCACC AGCTTCCTCC AGAAACTCTC AGACCTGGTG 
AGCAGAGATA CCCGACTTAG CAACTTGCTG AGGGAACAGT ATCCGGAGAG ACGGCGACAC 
CACAGGTTGG AGAATGGCTG TGGGAATGTA TCTCCTCAGA CCAAGGCCCG GAATGCACCT 
GTTTGGTTTC CTAACCATGA GGAACCCCTC CCAAGCAGCG CTGGGGCCAT CCGAGTCGGG 
GACAAAGTCC CCTATGATAT CTGCAGACCC GACCACTCGG TGCTGACCCT GCACCTGCCG 
GTGACAGCCT CCGTGAGGGA AGTGATGGCA GCTTTGGCCC ATGAGGACCA CTGGACCAAG 
GGGCAGGTGC TGGTAAAGGT CAATTCTGCC GGTGATGTCG TTGGCTTGCA GCCAGATGCC 
CGCGGTGTGG CCACATCCCT GGGGCTCAAT GAGCGGATCT TTGTTGTCGA CCCACAGGAA 
GTGCACGAGC TGACCCCACA CCCTGAGCAG CTGGGGCCCA CTCTGGGTTC TTCTGAGATG 
CTGGACCTAG TGAGTGCCAA GGACCTGGCA GGCCAGCTCA CAGAGCATGA CTGGAACCTC 
TTCAACAGGA TCCACCAGGT GGAGCTGATC CACTATGTAC TGGGCCCCCA GCACCTGCGG 
GACGTCACCA CTGCAAACCT GGAGCGCTTC ATGCGACGCT TCAACGAGCT GCAGTACTGG 
GTGGCCACCG AACTCTGTCT CTGCCCGGTT CCTGGCCCCC GGGCTCAGCT ACTCCGGAAG 
TTCATCAAGC TGGCAGCCCA CCTCAAGGAG CAGAAGAATC TCAACTCTTT CTTTGCGGTC 
ATGTTTGGCC TCAGCAACTC GGCCATCAGC CGCCTGGCCC ACACCTGGGA GCGTCTGCCC 
CATAAAGTAC GGAAGCTGTA CTCGGCCCTG GAAAGGTTGC TGGACCCTTC CTGGAACCAC 
CGAGTGTACC GATTGGCTCT CACCAAGCTC TCTCCTCCTG TCATCCCTTT CATGCCCCTG 
CTACTCAAAG ACATGACCTT CATCCATGAA GGGAACCACA CACTGGTAGA AAACCTCATC 
AACTTTGAGA AGATGCGAAT GATGGCCAGA GCCGTGCGGA TGCTCCACCA CTGCCGAAGC 
CACAGCACCG CGCCTCTATC ACCACTCAGA AGCCGGGTTT CCCACATCCA CGAGGACAGC 
CAGGCATCAA GAATCTCCAC ATGTTCCGAG CAGTCCCTGA GCACCCGGAG TCCAGCCAGC 
ACCTGGGCTT ATGTCCAGCA GCTGAAGGTC ATTGACAACC AGCGGGAACT GTCCCGCCTC 
TCCCGGGAAC TGGAACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef3 ( XM_008765779.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764001.1 Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa30747
Clone ID Related Accession (Same CDS sequence)	XM_008765779.1
Accession Version	XM_008765779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA AGAGAATGCA CCGTCCCCGG TGCTGCTCTT ACCAGCTAGT GTTCGAGCAC 
CGGCGCCCAA GCTGCATCCA GGGACTTCGC TGGACGCCAC TTACCAACAG TGAGGGCTCC 
CTGGACTTCA GAGTGAGCCT GGAGCAGGCC ACCACAGAGC ATGTGCACAA GGCCGGGAAG 
CTCCTGTACC GTCATCTCTT GGCAACGTAC CCTACCCTCA TCCGAGACAG AAAATACCAT 
CTGCGACTAC ATCGGCAGTG CTGCTCTGGC CGGGAGCTAG TGGATGGGAT CTTGGCTCTG 
GGTCTTGGGG TCCACTCACG GAGCCAAGCT GTGGGCATCT GCCAGGTGTT GCTGGATGAG 
GGTGCCCTTT GCCATGTAAA ACATGACTGG ACCTTCCAGG ACCGAGACGC CCAATTCTAC 
AGATTCCCTG GACCGGAGCC CCAGCCTGCA GGAACTCATG ACGTGGAAGA GGAGCTTGTT 
GAGGCAATGG CCCTACTCTC CCAGCGAGGG CCTGATGCCC TACTCACTGT TGCACTCCGG 
AAGTCCCCGG GTCAGCGTAC AGATGAAGAG CTGGACCTCA TCTTCGAGGA GCTGCTACAT 
ATCAAGGCGG TGGCTCACCT TTCTAACTCG GTGAAACGGG AACTAGCTGC TGTTCTGCTC 
TTTGAACCAC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGTACCTCA 
TGGTACATTA TCTGGAAGGG ATCTGTCAAT GTGGTGACCC ATGGCAAGGG GCTGGTGACC 
ACGTTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGACGC ACCTCGAGCA 
GCCACCATCA TCCTTCGAGA AAATAACTGT CACTTTCTGC GTGTGGACAA GCAGGACTTC 
AACCGCATCA TCAAGGATGT GGAAGCAAAA ACCATGAGAC TGGAAGAACA CGGCAAAGTG 
GTGTTAGTTT TGGAGAGAAC CTCTCAGGGT GCTGGCCCTT CCCGCCCTCC GACCCCAGGC 
AGGAACCGAT ATACGGTAAT GTCTGGCACC CCAGAGAAAA TCCTAGAACT CCTGTTGGAG 
GCTATGAGAC CGGATTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTG 
CTGACGCACA GTGTCTTCAT GCCCTGCACA CAGCTCTTTG CCGCCCTCCT GCACCACTTC 
CACGTGGAGC CATCAGAGCC TGCCGGGGGC AGCGAGCAGG AACGCAGCAC CTACATCTGC 
AACAAGAGGC AGCAGATTCT GCGTCTGGTC AGCCGGTGGG TGGCCCTCTA CAGCCCCATG 
CTCCGCTCAG ATCCCGTGGC CACCAGCTTC CTCCAGAAAC TCTCAGACCT GGTGAGCAGA 
GATACCCGAC TTAGCAACTT GCTGAGGGAA CAGTATCCGG AGAGACGGCG ACACCACAGG 
TTGGAGAATG GCTGTGGGAA TGTATCTCCT CAGACCAAGG CCCGGAATGC ACCTGTTTGG 
TTTCCTAACC ATGAGGAACC CCTCCCAAGC AGCGCTGGGG CCATCCGAGT CGGGGACAAA 
GTCCCCTATG ATATCTGCAG ACCCGACCAC TCGGTGCTGA CCCTGCACCT GCCGGTGACA 
GCCTCCGTGA GGGAAGTGAT GGCAGCTTTG GCCCATGAGG ACCACTGGAC CAAGGGGCAG 
GTGCTGGTAA AGGTCAATTC TGCCGGTGAT GTCGTTGGCT TGCAGCCAGA TGCCCGCGGT 
GTGGCCACAT CCCTGGGGCT CAATGAGCGG ATCTTTGTTG TCGACCCACA GGAAGTGCAC 
GAGCTGACCC CACACCCTGA GCAGCTGGGG CCCACTCTGG GTTCTTCTGA GATGCTGGAC 
CTAGTGAGTG CCAAGGACCT GGCAGGCCAG CTCACAGAGC ATGACTGGAA CCTCTTCAAC 
AGGATCCACC AGGTGGAGCT GATCCACTAT GTACTGGGCC CCCAGCACCT GCGGGACGTC 
ACCACTGCAA ACCTGGAGCG CTTCATGCGA CGCTTCAACG AGCTGCAGTA CTGGGTGGCC 
ACCGAACTCT GTCTCTGCCC GGTTCCTGGC CCCCGGGCTC AGCTACTCCG GAAGTTCATC 
AAGCTGGCAG CCCACCTCAA GGAGCAGAAG AATCTCAACT CTTTCTTTGC GGTCATGTTT 
GGCCTCAGCA ACTCGGCCAT CAGCCGCCTG GCCCACACCT GGGAGCGTCT GCCCCATAAA 
GTACGGAAGC TGTACTCGGC CCTGGAAAGG TTGCTGGACC CTTCCTGGAA CCACCGAGTG 
TACCGATTGG CTCTCACCAA GCTCTCTCCT CCTGTCATCC CTTTCATGCC CCTGCTACTC 
AAAGACATGA CCTTCATCCA TGAAGGGAAC CACACACTGG TAGAAAACCT CATCAACTTT 
GAGAAGATGC GAATGATGGC CAGAGCCGTG CGGATGCTCC ACCACTGCCG AAGCCACAGC 
ACCGCGCCTC TATCACCACT CAGAAGCCGG GTTTCCCACA TCCACGAGGA CAGCCAGGCA 
TCAAGAATCT CCACATGTTC CGAGCAGTCC CTGAGCACCC GGAGTCCAGC CAGCACCTGG 
GCTTATGTCC AGCAGCTGAA GGTCATTGAC AACCAGCGGG AACTGTCCCG CCTCTCCCGG 
GAACTGGAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764001.1
CDS	364..3018
Translation	MVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSPQTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQVLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_008765779.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765779.1

ORF Insert Sequence:

ATGGTGCTGA AGAGAATGCA CCGTCCCCGG TGCTGCTCTT ACCAGCTAGT GTTCGAGCAC 
CGGCGCCCAA GCTGCATCCA GGGACTTCGC TGGACGCCAC TTACCAACAG TGAGGGCTCC 
CTGGACTTCA GAGTGAGCCT GGAGCAGGCC ACCACAGAGC ATGTGCACAA GGCCGGGAAG 
CTCCTGTACC GTCATCTCTT GGCAACGTAC CCTACCCTCA TCCGAGACAG AAAATACCAT 
CTGCGACTAC ATCGGCAGTG CTGCTCTGGC CGGGAGCTAG TGGATGGGAT CTTGGCTCTG 
GGTCTTGGGG TCCACTCACG GAGCCAAGCT GTGGGCATCT GCCAGGTGTT GCTGGATGAG 
GGTGCCCTTT GCCATGTAAA ACATGACTGG ACCTTCCAGG ACCGAGACGC CCAATTCTAC 
AGATTCCCTG GACCGGAGCC CCAGCCTGCA GGAACTCATG ACGTGGAAGA GGAGCTTGTT 
GAGGCAATGG CCCTACTCTC CCAGCGAGGG CCTGATGCCC TACTCACTGT TGCACTCCGG 
AAGTCCCCGG GTCAGCGTAC AGATGAAGAG CTGGACCTCA TCTTCGAGGA GCTGCTACAT 
ATCAAGGCGG TGGCTCACCT TTCTAACTCG GTGAAACGGG AACTAGCTGC TGTTCTGCTC 
TTTGAACCAC ACAGCAAGGC AGGAACTGTG TTGTTCAGCC AGGGGGACAA GGGTACCTCA 
TGGTACATTA TCTGGAAGGG ATCTGTCAAT GTGGTGACCC ATGGCAAGGG GCTGGTGACC 
ACGTTGCACG AGGGAGATGA CTTTGGACAG CTGGCTCTGG TGAACGACGC ACCTCGAGCA 
GCCACCATCA TCCTTCGAGA AAATAACTGT CACTTTCTGC GTGTGGACAA GCAGGACTTC 
AACCGCATCA TCAAGGATGT GGAAGCAAAA ACCATGAGAC TGGAAGAACA CGGCAAAGTG 
GTGTTAGTTT TGGAGAGAAC CTCTCAGGGT GCTGGCCCTT CCCGCCCTCC GACCCCAGGC 
AGGAACCGAT ATACGGTAAT GTCTGGCACC CCAGAGAAAA TCCTAGAACT CCTGTTGGAG 
GCTATGAGAC CGGATTCCAG TGCTCATGAC CCAACAGAGA CATTCCTCAG TGACTTCCTG 
CTGACGCACA GTGTCTTCAT GCCCTGCACA CAGCTCTTTG CCGCCCTCCT GCACCACTTC 
CACGTGGAGC CATCAGAGCC TGCCGGGGGC AGCGAGCAGG AACGCAGCAC CTACATCTGC 
AACAAGAGGC AGCAGATTCT GCGTCTGGTC AGCCGGTGGG TGGCCCTCTA CAGCCCCATG 
CTCCGCTCAG ATCCCGTGGC CACCAGCTTC CTCCAGAAAC TCTCAGACCT GGTGAGCAGA 
GATACCCGAC TTAGCAACTT GCTGAGGGAA CAGTATCCGG AGAGACGGCG ACACCACAGG 
TTGGAGAATG GCTGTGGGAA TGTATCTCCT CAGACCAAGG CCCGGAATGC ACCTGTTTGG 
TTTCCTAACC ATGAGGAACC CCTCCCAAGC AGCGCTGGGG CCATCCGAGT CGGGGACAAA 
GTCCCCTATG ATATCTGCAG ACCCGACCAC TCGGTGCTGA CCCTGCACCT GCCGGTGACA 
GCCTCCGTGA GGGAAGTGAT GGCAGCTTTG GCCCATGAGG ACCACTGGAC CAAGGGGCAG 
GTGCTGGTAA AGGTCAATTC TGCCGGTGAT GTCGTTGGCT TGCAGCCAGA TGCCCGCGGT 
GTGGCCACAT CCCTGGGGCT CAATGAGCGG ATCTTTGTTG TCGACCCACA GGAAGTGCAC 
GAGCTGACCC CACACCCTGA GCAGCTGGGG CCCACTCTGG GTTCTTCTGA GATGCTGGAC 
CTAGTGAGTG CCAAGGACCT GGCAGGCCAG CTCACAGAGC ATGACTGGAA CCTCTTCAAC 
AGGATCCACC AGGTGGAGCT GATCCACTAT GTACTGGGCC CCCAGCACCT GCGGGACGTC 
ACCACTGCAA ACCTGGAGCG CTTCATGCGA CGCTTCAACG AGCTGCAGTA CTGGGTGGCC 
ACCGAACTCT GTCTCTGCCC GGTTCCTGGC CCCCGGGCTC AGCTACTCCG GAAGTTCATC 
AAGCTGGCAG CCCACCTCAA GGAGCAGAAG AATCTCAACT CTTTCTTTGC GGTCATGTTT 
GGCCTCAGCA ACTCGGCCAT CAGCCGCCTG GCCCACACCT GGGAGCGTCT GCCCCATAAA 
GTACGGAAGC TGTACTCGGC CCTGGAAAGG TTGCTGGACC CTTCCTGGAA CCACCGAGTG 
TACCGATTGG CTCTCACCAA GCTCTCTCCT CCTGTCATCC CTTTCATGCC CCTGCTACTC 
AAAGACATGA CCTTCATCCA TGAAGGGAAC CACACACTGG TAGAAAACCT CATCAACTTT 
GAGAAGATGC GAATGATGGC CAGAGCCGTG CGGATGCTCC ACCACTGCCG AAGCCACAGC 
ACCGCGCCTC TATCACCACT CAGAAGCCGG GTTTCCCACA TCCACGAGGA CAGCCAGGCA 
TCAAGAATCT CCACATGTTC CGAGCAGTCC CTGAGCACCC GGAGTCCAGC CAGCACCTGG 
GCTTATGTCC AGCAGCTGAA GGTCATTGAC AACCAGCGGG AACTGTCCCG CCTCTCCCGG 
GAACTGGAAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef3 ( XM_008765778.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764000.1 Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43501
Clone ID Related Accession (Same CDS sequence)	XM_008765778.2
Accession Version	XM_008765778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGTC CAAGTCCAGA GGACGCTAAG CCTGGGGTGG TGGTAGGAAG GTCCAGCTCC 
TCCGGCCGAT GGCTGTCCAC TTTCCTCCCC CCATCACAGG TCAGCTGGCC AGGTGAGAAC 
CACTGGCAGG TGGGCCCAGC TGTGGTGGAG AGTCCAGCTG TGGGGGCACC GCAGGTGGGA 
GGTCTCCCGG ACGTGGTTCC GGAGGGCACG CTGCTCAATA TGGTGCTGAA GAGAATGCAC 
CGTCCCCGGT GCTGCTCTTA CCAGCTAGTG TTCGAGCACC GGCGCCCAAG CTGCATCCAG 
GGACTTCGCT GGACGCCACT TACCAACAGT GAGGGCTCCC TGGACTTCAG AGTGAGCCTG 
GAGCAGGCCA CCACAGAGCA TGTGCACAAG GCCGGGAAGC TCCTGTACCG TCATCTCTTG 
GCAACGTACC CTACCCTCAT CCGAGACAGA AAATACCATC TGCGACTACA TCGGCAGTGC 
TGCTCTGGCC GGGAGCTAGT GGATGGGATC TTGGCTCTGG GTCTTGGGGT CCACTCACGG 
AGCCAAGCTG TGGGCATCTG CCAGGTGTTG CTGGATGAGG GTGCCCTTTG CCATGTAAAA 
CATGACTGGA CCTTCCAGGA CCGAGACGCC CAATTCTACA GATTCCCTGG ACCGGAGCCC 
CAGCCTGCAG GAACTCATGA CGTGGAAGAG GAGCTTGTTG AGGCAATGGC CCTACTCTCC 
CAGCGAGGGC CTGATGCCCT ACTCACTGTT GCACTCCGGA AGTCCCCGGG TCAGCGTACA 
GATGAAGAGC TGGACCTCAT CTTCGAGGAG CTGCTACATA TCAAGGCGGT GGCTCACCTT 
TCTAACTCGG TGAAACGGGA ACTAGCTGCT GTTCTGCTCT TTGAACCACA CAGCAAGGCA 
GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCAT GGTACATTAT CTGGAAGGGA 
TCTGTCAATG TGGTGACCCA TGGCAAGGGG CTGGTGACCA CGTTGCACGA GGGAGATGAC 
TTTGGACAGC TGGCTCTGGT GAACGACGCA CCTCGAGCAG CCACCATCAT CCTTCGAGAA 
AATAACTGTC ACTTTCTGCG TGTGGACAAG CAGGACTTCA ACCGCATCAT CAAGGATGTG 
GAAGCAAAAA CCATGAGACT GGAAGAACAC GGCAAAGTGG TGTTAGTTTT GGAGAGAACC 
TCTCAGGGTG CTGGCCCTTC CCGCCCTCCG ACCCCAGGCA GGAACCGATA TACGGTAATG 
TCTGGCACCC CAGAGAAAAT CCTAGAACTC CTGTTGGAGG CTATGAGACC GGATTCCAGT 
GCTCATGACC CAACAGAGAC ATTCCTCAGT GACTTCCTGC TGACGCACAG TGTCTTCATG 
CCCTGCACAC AGCTCTTTGC CGCCCTCCTG CACCACTTCC ACGTGGAGCC ATCAGAGCCT 
GCCGGGGGCA GCGAGCAGGA ACGCAGCACC TACATCTGCA ACAAGAGGCA GCAGATTCTG 
CGTCTGGTCA GCCGGTGGGT GGCCCTCTAC AGCCCCATGC TCCGCTCAGA TCCCGTGGCC 
ACCAGCTTCC TCCAGAAACT CTCAGACCTG GTGAGCAGAG ATACCCGACT TAGCAACTTG 
CTGAGGGAAC AGTATCCGGA GAGACGGCGA CACCACAGGT TGGAGAATGG CTGTGGGAAT 
GTATCTCCTC AGACCAAGGC CCGGAATGCA CCTGTTTGGT TTCCTAACCA TGAGGAACCC 
CTCCCAAGCA GCGCTGGGGC CATCCGAGTC GGGGACAAAG TCCCCTATGA TATCTGCAGA 
CCCGACCACT CGGTGCTGAC CCTGCACCTG CCGGTGACAG CCTCCGTGAG GGAAGTGATG 
GCAGCTTTGG CCCATGAGGA CCACTGGACC AAGGGGCAGG TGCTGGTAAA GGTCAATTCT 
GCCGGTGATG TCGTTGGCTT GCAGCCAGAT GCCCGCGGTG TGGCCACATC CCTGGGGCTC 
AATGAGCGGA TCTTTGTTGT CGACCCACAG GAAGTGCACG AGCTGACCCC ACACCCTGAG 
CAGCTGGGGC CCACTCTGGG TTCTTCTGAG ATGCTGGACC TAGTGAGTGC CAAGGACCTG 
GCAGGCCAGC TCACAGAGCA TGACTGGAAC CTCTTCAACA GGATCCACCA GGTGGAGCTG 
ATCCACTATG TACTGGGCCC CCAGCACCTG CGGGACGTCA CCACTGCAAA CCTGGAGCGC 
TTCATGCGAC GCTTCAACGA GCTGCAGTAC TGGGTGGCCA CCGAACTCTG TCTCTGCCCG 
GTTCCTGGCC CCCGGGCTCA GCTACTCCGG AAGTTCATCA AGCTGGCAGC CCACCTCAAG 
GAGCAGAAGA ATCTCAACTC TTTCTTTGCG GTCATGTTTG GCCTCAGCAA CTCGGCCATC 
AGCCGCCTGG CCCACACCTG GGAGCGTCTG CCCCATAAAG TACGGAAGCT GTACTCGGCC 
CTGGAAAGGT TGCTGGACCC TTCCTGGAAC CACCGAGTGT ACCGATTGGC TCTCACCAAG 
CTCTCTCCTC CTGTCATCCC TTTCATGCCC CTGCTACTCA AAGACATGAC CTTCATCCAT 
GAAGGGAACC ACACACTGGT AGAAAACCTC ATCAACTTTG AGAAGATGCG AATGATGGCC 
AGAGCCGTGC GGATGCTCCA CCACTGCCGA AGCCACAGCA CCGCGCCTCT ATCACCACTC 
AGAAGCCGGG TTTCCCACAT CCACGAGGAC AGCCAGGCAT CAAGAATCTC CACATGTTCC 
GAGCAGTCCC TGAGCACCCG GAGTCCAGCC AGCACCTGGG CTTATGTCCA GCAGCTGAAG 
GTCATTGACA ACCAGCGGGA ACTGTCCCGC CTCTCCCGGG AACTGGAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764000.1
CDS	277..3150
Translation	MTSPSPEDAKPGVVVGRSSSSGRWLSTFLPPSQVSWPGENHWQVGPAVVESPAVGAPQVGGLPDVVPEGTLLNMVLKRMHRPRCCSYQLVFEHRRPSCIQGLRWTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSPQTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQVLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_008765778.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765778.2

ORF Insert Sequence:

ATGACCAGTC CAAGTCCAGA GGACGCTAAG CCTGGGGTGG TGGTAGGAAG GTCCAGCTCC 
TCCGGCCGAT GGCTGTCCAC TTTCCTCCCC CCATCACAGG TCAGCTGGCC AGGTGAGAAC 
CACTGGCAGG TGGGCCCAGC TGTGGTGGAG AGTCCAGCTG TGGGGGCACC GCAGGTGGGA 
GGTCTCCCGG ACGTGGTTCC GGAGGGCACG CTGCTCAATA TGGTGCTGAA GAGAATGCAC 
CGTCCCCGGT GCTGCTCTTA CCAGCTAGTG TTCGAGCACC GGCGCCCAAG CTGCATCCAG 
GGACTTCGCT GGACGCCACT TACCAACAGT GAGGGCTCCC TGGACTTCAG AGTGAGCCTG 
GAGCAGGCCA CCACAGAGCA TGTGCACAAG GCCGGGAAGC TCCTGTACCG TCATCTCTTG 
GCAACGTACC CTACCCTCAT CCGAGACAGA AAATACCATC TGCGACTACA TCGGCAGTGC 
TGCTCTGGCC GGGAGCTAGT GGATGGGATC TTGGCTCTGG GTCTTGGGGT CCACTCACGG 
AGCCAAGCTG TGGGCATCTG CCAGGTGTTG CTGGATGAGG GTGCCCTTTG CCATGTAAAA 
CATGACTGGA CCTTCCAGGA CCGAGACGCC CAATTCTACA GATTCCCTGG ACCGGAGCCC 
CAGCCTGCAG GAACTCATGA CGTGGAAGAG GAGCTTGTTG AGGCAATGGC CCTACTCTCC 
CAGCGAGGGC CTGATGCCCT ACTCACTGTT GCACTCCGGA AGTCCCCGGG TCAGCGTACA 
GATGAAGAGC TGGACCTCAT CTTCGAGGAG CTGCTACATA TCAAGGCGGT GGCTCACCTT 
TCTAACTCGG TGAAACGGGA ACTAGCTGCT GTTCTGCTCT TTGAACCACA CAGCAAGGCA 
GGAACTGTGT TGTTCAGCCA GGGGGACAAG GGTACCTCAT GGTACATTAT CTGGAAGGGA 
TCTGTCAATG TGGTGACCCA TGGCAAGGGG CTGGTGACCA CGTTGCACGA GGGAGATGAC 
TTTGGACAGC TGGCTCTGGT GAACGACGCA CCTCGAGCAG CCACCATCAT CCTTCGAGAA 
AATAACTGTC ACTTTCTGCG TGTGGACAAG CAGGACTTCA ACCGCATCAT CAAGGATGTG 
GAAGCAAAAA CCATGAGACT GGAAGAACAC GGCAAAGTGG TGTTAGTTTT GGAGAGAACC 
TCTCAGGGTG CTGGCCCTTC CCGCCCTCCG ACCCCAGGCA GGAACCGATA TACGGTAATG 
TCTGGCACCC CAGAGAAAAT CCTAGAACTC CTGTTGGAGG CTATGAGACC GGATTCCAGT 
GCTCATGACC CAACAGAGAC ATTCCTCAGT GACTTCCTGC TGACGCACAG TGTCTTCATG 
CCCTGCACAC AGCTCTTTGC CGCCCTCCTG CACCACTTCC ACGTGGAGCC ATCAGAGCCT 
GCCGGGGGCA GCGAGCAGGA ACGCAGCACC TACATCTGCA ACAAGAGGCA GCAGATTCTG 
CGTCTGGTCA GCCGGTGGGT GGCCCTCTAC AGCCCCATGC TCCGCTCAGA TCCCGTGGCC 
ACCAGCTTCC TCCAGAAACT CTCAGACCTG GTGAGCAGAG ATACCCGACT TAGCAACTTG 
CTGAGGGAAC AGTATCCGGA GAGACGGCGA CACCACAGGT TGGAGAATGG CTGTGGGAAT 
GTATCTCCTC AGACCAAGGC CCGGAATGCA CCTGTTTGGT TTCCTAACCA TGAGGAACCC 
CTCCCAAGCA GCGCTGGGGC CATCCGAGTC GGGGACAAAG TCCCCTATGA TATCTGCAGA 
CCCGACCACT CGGTGCTGAC CCTGCACCTG CCGGTGACAG CCTCCGTGAG GGAAGTGATG 
GCAGCTTTGG CCCATGAGGA CCACTGGACC AAGGGGCAGG TGCTGGTAAA GGTCAATTCT 
GCCGGTGATG TCGTTGGCTT GCAGCCAGAT GCCCGCGGTG TGGCCACATC CCTGGGGCTC 
AATGAGCGGA TCTTTGTTGT CGACCCACAG GAAGTGCACG AGCTGACCCC ACACCCTGAG 
CAGCTGGGGC CCACTCTGGG TTCTTCTGAG ATGCTGGACC TAGTGAGTGC CAAGGACCTG 
GCAGGCCAGC TCACAGAGCA TGACTGGAAC CTCTTCAACA GGATCCACCA GGTGGAGCTG 
ATCCACTATG TACTGGGCCC CCAGCACCTG CGGGACGTCA CCACTGCAAA CCTGGAGCGC 
TTCATGCGAC GCTTCAACGA GCTGCAGTAC TGGGTGGCCA CCGAACTCTG TCTCTGCCCG 
GTTCCTGGCC CCCGGGCTCA GCTACTCCGG AAGTTCATCA AGCTGGCAGC CCACCTCAAG 
GAGCAGAAGA ATCTCAACTC TTTCTTTGCG GTCATGTTTG GCCTCAGCAA CTCGGCCATC 
AGCCGCCTGG CCCACACCTG GGAGCGTCTG CCCCATAAAG TACGGAAGCT GTACTCGGCC 
CTGGAAAGGT TGCTGGACCC TTCCTGGAAC CACCGAGTGT ACCGATTGGC TCTCACCAAG 
CTCTCTCCTC CTGTCATCCC TTTCATGCCC CTGCTACTCA AAGACATGAC CTTCATCCAT 
GAAGGGAACC ACACACTGGT AGAAAACCTC ATCAACTTTG AGAAGATGCG AATGATGGCC 
AGAGCCGTGC GGATGCTCCA CCACTGCCGA AGCCACAGCA CCGCGCCTCT ATCACCACTC 
AGAAGCCGGG TTTCCCACAT CCACGAGGAC AGCCAGGCAT CAAGAATCTC CACATGTTCC 
GAGCAGTCCC TGAGCACCCG GAGTCCAGCC AGCACCTGGG CTTATGTCCA GCAGCTGAAG 
GTCATTGACA ACCAGCGGGA ACTGTCCCGC CTCTCCCGGG AACTGGAACC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef3 ( XM_008765780.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764002.1 Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X4, mRNA.

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CloneID	ORa43502
Clone ID Related Accession (Same CDS sequence)	XM_008765780.2
Accession Version	XM_008765780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGAAG GAACTGGTCT GACGCCACTT ACCAACAGTG AGGGCTCCCT GGACTTCAGA 
GTGAGCCTGG AGCAGGCCAC CACAGAGCAT GTGCACAAGG CCGGGAAGCT CCTGTACCGT 
CATCTCTTGG CAACGTACCC TACCCTCATC CGAGACAGAA AATACCATCT GCGACTACAT 
CGGCAGTGCT GCTCTGGCCG GGAGCTAGTG GATGGGATCT TGGCTCTGGG TCTTGGGGTC 
CACTCACGGA GCCAAGCTGT GGGCATCTGC CAGGTGTTGC TGGATGAGGG TGCCCTTTGC 
CATGTAAAAC ATGACTGGAC CTTCCAGGAC CGAGACGCCC AATTCTACAG ATTCCCTGGA 
CCGGAGCCCC AGCCTGCAGG AACTCATGAC GTGGAAGAGG AGCTTGTTGA GGCAATGGCC 
CTACTCTCCC AGCGAGGGCC TGATGCCCTA CTCACTGTTG CACTCCGGAA GTCCCCGGGT 
CAGCGTACAG ATGAAGAGCT GGACCTCATC TTCGAGGAGC TGCTACATAT CAAGGCGGTG 
GCTCACCTTT CTAACTCGGT GAAACGGGAA CTAGCTGCTG TTCTGCTCTT TGAACCACAC 
AGCAAGGCAG GAACTGTGTT GTTCAGCCAG GGGGACAAGG GTACCTCATG GTACATTATC 
TGGAAGGGAT CTGTCAATGT GGTGACCCAT GGCAAGGGGC TGGTGACCAC GTTGCACGAG 
GGAGATGACT TTGGACAGCT GGCTCTGGTG AACGACGCAC CTCGAGCAGC CACCATCATC 
CTTCGAGAAA ATAACTGTCA CTTTCTGCGT GTGGACAAGC AGGACTTCAA CCGCATCATC 
AAGGATGTGG AAGCAAAAAC CATGAGACTG GAAGAACACG GCAAAGTGGT GTTAGTTTTG 
GAGAGAACCT CTCAGGGTGC TGGCCCTTCC CGCCCTCCGA CCCCAGGCAG GAACCGATAT 
ACGGTAATGT CTGGCACCCC AGAGAAAATC CTAGAACTCC TGTTGGAGGC TATGAGACCG 
GATTCCAGTG CTCATGACCC AACAGAGACA TTCCTCAGTG ACTTCCTGCT GACGCACAGT 
GTCTTCATGC CCTGCACACA GCTCTTTGCC GCCCTCCTGC ACCACTTCCA CGTGGAGCCA 
TCAGAGCCTG CCGGGGGCAG CGAGCAGGAA CGCAGCACCT ACATCTGCAA CAAGAGGCAG 
CAGATTCTGC GTCTGGTCAG CCGGTGGGTG GCCCTCTACA GCCCCATGCT CCGCTCAGAT 
CCCGTGGCCA CCAGCTTCCT CCAGAAACTC TCAGACCTGG TGAGCAGAGA TACCCGACTT 
AGCAACTTGC TGAGGGAACA GTATCCGGAG AGACGGCGAC ACCACAGGTT GGAGAATGGC 
TGTGGGAATG TATCTCCTCA GACCAAGGCC CGGAATGCAC CTGTTTGGTT TCCTAACCAT 
GAGGAACCCC TCCCAAGCAG CGCTGGGGCC ATCCGAGTCG GGGACAAAGT CCCCTATGAT 
ATCTGCAGAC CCGACCACTC GGTGCTGACC CTGCACCTGC CGGTGACAGC CTCCGTGAGG 
GAAGTGATGG CAGCTTTGGC CCATGAGGAC CACTGGACCA AGGGGCAGGT GCTGGTAAAG 
GTCAATTCTG CCGGTGATGT CGTTGGCTTG CAGCCAGATG CCCGCGGTGT GGCCACATCC 
CTGGGGCTCA ATGAGCGGAT CTTTGTTGTC GACCCACAGG AAGTGCACGA GCTGACCCCA 
CACCCTGAGC AGCTGGGGCC CACTCTGGGT TCTTCTGAGA TGCTGGACCT AGTGAGTGCC 
AAGGACCTGG CAGGCCAGCT CACAGAGCAT GACTGGAACC TCTTCAACAG GATCCACCAG 
GTGGAGCTGA TCCACTATGT ACTGGGCCCC CAGCACCTGC GGGACGTCAC CACTGCAAAC 
CTGGAGCGCT TCATGCGACG CTTCAACGAG CTGCAGTACT GGGTGGCCAC CGAACTCTGT 
CTCTGCCCGG TTCCTGGCCC CCGGGCTCAG CTACTCCGGA AGTTCATCAA GCTGGCAGCC 
CACCTCAAGG AGCAGAAGAA TCTCAACTCT TTCTTTGCGG TCATGTTTGG CCTCAGCAAC 
TCGGCCATCA GCCGCCTGGC CCACACCTGG GAGCGTCTGC CCCATAAAGT ACGGAAGCTG 
TACTCGGCCC TGGAAAGGTT GCTGGACCCT TCCTGGAACC ACCGAGTGTA CCGATTGGCT 
CTCACCAAGC TCTCTCCTCC TGTCATCCCT TTCATGCCCC TGCTACTCAA AGACATGACC 
TTCATCCATG AAGGGAACCA CACACTGGTA GAAAACCTCA TCAACTTTGA GAAGATGCGA 
ATGATGGCCA GAGCCGTGCG GATGCTCCAC CACTGCCGAA GCCACAGCAC CGCGCCTCTA 
TCACCACTCA GAAGCCGGGT TTCCCACATC CACGAGGACA GCCAGGCATC AAGAATCTCC 
ACATGTTCCG AGCAGTCCCT GAGCACCCGG AGTCCAGCCA GCACCTGGGC TTATGTCCAG 
CAGCTGAAGG TCATTGACAA CCAGCGGGAA CTGTCCCGCC TCTCCCGGGA ACTGGAACCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764002.1
CDS	792..3374
Translation	MTEGTGLTPLTNSEGSLDFRVSLEQATTEHVHKAGKLLYRHLLATYPTLIRDRKYHLRLHRQCCSGRELVDGILALGLGVHSRSQAVGICQVLLDEGALCHVKHDWTFQDRDAQFYRFPGPEPQPAGTHDVEEELVEAMALLSQRGPDALLTVALRKSPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILRENNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERTSQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMRPDSSAHDPTETFLSDFLLTHSVFMPCTQLFAALLHHFHVEPSEPAGGSEQERSTYICNKRQQILRLVSRWVALYSPMLRSDPVATSFLQKLSDLVSRDTRLSNLLREQYPERRRHHRLENGCGNVSPQTKARNAPVWFPNHEEPLPSSAGAIRVGDKVPYDICRPDHSVLTLHLPVTASVREVMAALAHEDHWTKGQVLVKVNSAGDVVGLQPDARGVATSLGLNERIFVVDPQEVHELTPHPEQLGPTLGSSEMLDLVSAKDLAGQLTEHDWNLFNRIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALTKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAVRMLHHCRSHSTAPLSPLRSRVSHIHEDSQASRISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Target ORF information:

RefSeq Version	XM_008765780.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 3 (Rapgef3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765780.2

ORF Insert Sequence:

ATGACCGAAG GAACTGGTCT GACGCCACTT ACCAACAGTG AGGGCTCCCT GGACTTCAGA 
GTGAGCCTGG AGCAGGCCAC CACAGAGCAT GTGCACAAGG CCGGGAAGCT CCTGTACCGT 
CATCTCTTGG CAACGTACCC TACCCTCATC CGAGACAGAA AATACCATCT GCGACTACAT 
CGGCAGTGCT GCTCTGGCCG GGAGCTAGTG GATGGGATCT TGGCTCTGGG TCTTGGGGTC 
CACTCACGGA GCCAAGCTGT GGGCATCTGC CAGGTGTTGC TGGATGAGGG TGCCCTTTGC 
CATGTAAAAC ATGACTGGAC CTTCCAGGAC CGAGACGCCC AATTCTACAG ATTCCCTGGA 
CCGGAGCCCC AGCCTGCAGG AACTCATGAC GTGGAAGAGG AGCTTGTTGA GGCAATGGCC 
CTACTCTCCC AGCGAGGGCC TGATGCCCTA CTCACTGTTG CACTCCGGAA GTCCCCGGGT 
CAGCGTACAG ATGAAGAGCT GGACCTCATC TTCGAGGAGC TGCTACATAT CAAGGCGGTG 
GCTCACCTTT CTAACTCGGT GAAACGGGAA CTAGCTGCTG TTCTGCTCTT TGAACCACAC 
AGCAAGGCAG GAACTGTGTT GTTCAGCCAG GGGGACAAGG GTACCTCATG GTACATTATC 
TGGAAGGGAT CTGTCAATGT GGTGACCCAT GGCAAGGGGC TGGTGACCAC GTTGCACGAG 
GGAGATGACT TTGGACAGCT GGCTCTGGTG AACGACGCAC CTCGAGCAGC CACCATCATC 
CTTCGAGAAA ATAACTGTCA CTTTCTGCGT GTGGACAAGC AGGACTTCAA CCGCATCATC 
AAGGATGTGG AAGCAAAAAC CATGAGACTG GAAGAACACG GCAAAGTGGT GTTAGTTTTG 
GAGAGAACCT CTCAGGGTGC TGGCCCTTCC CGCCCTCCGA CCCCAGGCAG GAACCGATAT 
ACGGTAATGT CTGGCACCCC AGAGAAAATC CTAGAACTCC TGTTGGAGGC TATGAGACCG 
GATTCCAGTG CTCATGACCC AACAGAGACA TTCCTCAGTG ACTTCCTGCT GACGCACAGT 
GTCTTCATGC CCTGCACACA GCTCTTTGCC GCCCTCCTGC ACCACTTCCA CGTGGAGCCA 
TCAGAGCCTG CCGGGGGCAG CGAGCAGGAA CGCAGCACCT ACATCTGCAA CAAGAGGCAG 
CAGATTCTGC GTCTGGTCAG CCGGTGGGTG GCCCTCTACA GCCCCATGCT CCGCTCAGAT 
CCCGTGGCCA CCAGCTTCCT CCAGAAACTC TCAGACCTGG TGAGCAGAGA TACCCGACTT 
AGCAACTTGC TGAGGGAACA GTATCCGGAG AGACGGCGAC ACCACAGGTT GGAGAATGGC 
TGTGGGAATG TATCTCCTCA GACCAAGGCC CGGAATGCAC CTGTTTGGTT TCCTAACCAT 
GAGGAACCCC TCCCAAGCAG CGCTGGGGCC ATCCGAGTCG GGGACAAAGT CCCCTATGAT 
ATCTGCAGAC CCGACCACTC GGTGCTGACC CTGCACCTGC CGGTGACAGC CTCCGTGAGG 
GAAGTGATGG CAGCTTTGGC CCATGAGGAC CACTGGACCA AGGGGCAGGT GCTGGTAAAG 
GTCAATTCTG CCGGTGATGT CGTTGGCTTG CAGCCAGATG CCCGCGGTGT GGCCACATCC 
CTGGGGCTCA ATGAGCGGAT CTTTGTTGTC GACCCACAGG AAGTGCACGA GCTGACCCCA 
CACCCTGAGC AGCTGGGGCC CACTCTGGGT TCTTCTGAGA TGCTGGACCT AGTGAGTGCC 
AAGGACCTGG CAGGCCAGCT CACAGAGCAT GACTGGAACC TCTTCAACAG GATCCACCAG 
GTGGAGCTGA TCCACTATGT ACTGGGCCCC CAGCACCTGC GGGACGTCAC CACTGCAAAC 
CTGGAGCGCT TCATGCGACG CTTCAACGAG CTGCAGTACT GGGTGGCCAC CGAACTCTGT 
CTCTGCCCGG TTCCTGGCCC CCGGGCTCAG CTACTCCGGA AGTTCATCAA GCTGGCAGCC 
CACCTCAAGG AGCAGAAGAA TCTCAACTCT TTCTTTGCGG TCATGTTTGG CCTCAGCAAC 
TCGGCCATCA GCCGCCTGGC CCACACCTGG GAGCGTCTGC CCCATAAAGT ACGGAAGCTG 
TACTCGGCCC TGGAAAGGTT GCTGGACCCT TCCTGGAACC ACCGAGTGTA CCGATTGGCT 
CTCACCAAGC TCTCTCCTCC TGTCATCCCT TTCATGCCCC TGCTACTCAA AGACATGACC 
TTCATCCATG AAGGGAACCA CACACTGGTA GAAAACCTCA TCAACTTTGA GAAGATGCGA 
ATGATGGCCA GAGCCGTGCG GATGCTCCAC CACTGCCGAA GCCACAGCAC CGCGCCTCTA 
TCACCACTCA GAAGCCGGGT TTCCCACATC CACGAGGACA GCCAGGCATC AAGAATCTCC 
ACATGTTCCG AGCAGTCCCT GAGCACCCGG AGTCCAGCCA GCACCTGGGC TTATGTCCAG 
CAGCTGAAGG TCATTGACAA CCAGCGGGAA CTGTCCCGCC TCTCCCGGGA ACTGGAACCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Early effects of Epac depend on the fine-tuning of the sarcoplasmic reticulum Ca
Journal of molecular and cellular cardiology1141-9(2017 Oct)
Lezcano N,Mari?ngelo JIE,Vittone L,Wehrens XHT,Said M,Mundi?a-Weilenmann C

Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.
Biochemical and biophysical research communications485(2)355-359(2017 04)
Sun W,Jiao W,Huang Y,Li R,Zhang Z,Wang J,Lei T

Epac-Rap1-activated mesenchymal stem cells improve cardiac function in rat model of myocardial infarction.
Cardiovascular therapeutics35(2)(2017 Apr)
Khan I,Ali A,Akhter MA,Naeem N,Chotani MA,Iqbal H,Kabir N,Atiq M,Salim A

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Rapgef3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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