Ncan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ncan
Entrez Gene ID	58982
Full Name	neurocan
Synonyms	Cspg3
General protein information	Preferred Names neurocan Names neurocan core protein 245 kDa early postnatal core glycoprotein chondroitin sulfate proteoglycan 3 neurocan core protein-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	chondroitin sulfate proteoglycan that may play a role in cell adhesion and cell migration [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031653.2	NP_113841.2	neurocan core protein precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCAN	NP_004377.2
Mus musculus (house mouse)	Ncan	NP_031815.2
Canis lupus familiaris (dog)	NCAN	XP_541924.3
Bos taurus (cattle)	NCAN	NP_001180011.1
Rattus norvegicus (Norway rat)	Ncan	NP_113841.2
Pan troglodytes (chimpanzee)	NCAN	NP_001029342.1
	NCAN	XP_002808228.1
Rattus norvegicus (Norway rat)	LOC102550316	XP_006253042.1

Gene Ontology
Bibliography

Related articles in PubMed

Olfactory ensheathing cells (OECs) degrade neurocan in injured spinal cord by secreting matrix metalloproteinase-2 in a rat contusion model.
Yui S, Fujita N, Chung CS, Morita M, Nishimura R
The Japanese journal of veterinary research62(4)151-62(2014 Nov)

Upregulation of CSPG3 accompanies neuronal progenitor proliferation and migration in EAE.
Sajad M, Zargan J, Chawla R, Umar S, Khan HA
Journal of molecular neuroscience : MN43(3)531-40(2011 Mar)

Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain.
Shen LH, Li Y, Gao Q, Savant-Bhonsale S, Chopp M
Glia56(16)1747-54(2008 Dec)

Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum.
Cheng D, Hoogenraad CC, Rush J, Ramm E, Schlager MA, Duong DM, Xu P, Wijayawardana SR, Hanfelt J, Nakagawa T, Sheng M, Peng J
Molecular & cellular proteomics : MCP5(6)1158-70(2006 Jun)

Composition of perineuronal net extracellular matrix in rat brain: a different disaccharide composition for the net-associated proteoglycans.
Deepa SS, Carulli D, Galtrey C, Rhodes K, Fukuda J, Mikami T, Sugahara K, Fawcett JW
The Journal of biological chemistry281(26)17789-800(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In vitro results showed that MMP-2 was the dominant MMP expressed by olfactory ensheathing cells (OECs). In vivo results revealed that transplanted OECs secreted MMP-2 in injured spinal cord and that the transplantation decreased neurocan expression.
Title: Olfactory ensheathing cells (OECs) degrade neurocan in injured spinal cord by secreting matrix metalloproteinase-2 in a rat contusion model.

Results report for the first time that extracellular matrix chondroitin sulphate proteoglycan, CSPG3 (neurocan) is upregulated after primary inflammatory injury.
Title: Upregulation of CSPG3 accompanies neuronal progenitor proliferation and migration in EAE.

Our data suggest that BMSCs promote axonal regeneration by reducing neurocan expression in peri-infarct astrocytes.
Title: Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain.

accumulation of the full-length neurocan produced by reactive astrocytes may be one of the processes for tissue repair and reconstruction of neural networks
Title: Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain.

Data suggest that the reported temporal co-expression of cartilage link protein and neurocan in developing brain implicates the possibility of a cooperative function of these molecules.
Title: Cartilage link protein interacts with neurocan, which shows hyaluronan binding characteristics different from CD44 and TSG-6.

The following Ncan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ncan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00031	NM_031653.2 Latest version!	Rattus norvegicus neurocan (Ncan), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$923.30 $1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ncan ( NM_031653.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113841.2 Rattus norvegicus neurocan (Ncan), mRNA.

Price & Availability↑

CloneID	ORa00031
Clone ID Related Accession (Same CDS sequence)	NM_031653.2
Accession Version	NM_031653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	neurocan core protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134063.4. On Sep 12, 2012 this sequence version replaced NM_031653.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M97161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGCCG AATCTGTCTG GGCCTCAGGC CTCCTGGTGC TGTGGCTGCT TCTCCTAGTG 
TCTGGGGATC AGGACACACA GGACACCACC ACCACGGAAA AGGGGCTTCA CATGCTGAAG 
TCGGGGTCAG GACCCATCCA GGCTGCTTTG GCAGAGTTAG TGGCCCTGCC CTGCTTCTTT 
ACCCTTCAAC CACGGCAAAG CCCCCTGGGA GACATTCCTC GGATCAAGTG GACGAAAGTT 
CAGACTGCAT CAGGCCAGCG ACAGGATTTG CCAATCTTGG TGGCCAAAGA CAACGTGGTG 
CGTGTGGCCA AGGGCTGGCA GGGACGGGTG TCATTGCCTG CCTATCCCCG GCACAGAGCC 
AATGCTACAC TTCTGTTGGG GCCACTGCGA GCCAGTGACT CTGGGCTGTA TCGCTGCCAA 
GTGGTGAAGG GTATCGAGGA TGAGCAGGAC CTGGTAACCC TGGAAGTGAC AGGCGTCGTG 
TTCCATTATC GGGCGGCCCG GGACCGCTAT GCGTTGACCT TCGCTGAGGC CCAGGAGGCT 
TGTCACCTGA GCTCCGCTAC CATTGCGGCT CCAAGGCACC TGCAGGCTGC GTTCGAAGAT 
GGCTTTGACA ACTGCGATGC GGGCTGGCTC TCAGACCGCA CGGTCCGGTA CCCGATCACT 
CAGTCGCGTC CCGGTTGCTA TGGTGATCGC AGCAGCCTGC CAGGTGTCCG GAGCTACGGG 
AGACGCGACC CGCAGGAACT CTACGATGTC TACTGCTTTG CCCGCGAGCT AGGGGGTGAA 
GTCTTTTACG TGGGCCCCGC CCGCCGACTG ACCCTGGCGG GGGCGCGGGC ACTGTGTCAG 
CGTCAGGGTG CAGCGCTGGC CTCCGTGGGA CAGTTGCACC TGGCCTGGCA CGAGGGCCTG 
GACCAGTGCG ACCCGGGCTG GCTGGCAGAC GGCAGCGTGC GCTACCCGAT CCAGACTCCA 
CGGCGGCGTT GCGGGGGCTC CGCTCCAGGT GTGCGCACAG TGTACCGCTT CGCCAACCGC 
ACTGGCTTTC CTGCGCCAGG AGCCCGCTTC GACGCCTACT GCTTCCGAGC TCATCACCAT 
ACACCACAAC GTGGAGACTC CGAGATCCCC TCATCTGGAG ATGAGGGGGA GATTGTGTCA 
GCAGAGGGGC CGCCAGCCCC AGAACTAAAG CCCAGATTGG GGGAGCAGGA GGTGATAACA 
CCTGACTTCC AGGAACCTCT CGTATCCAGT GGAGAAGATG AACCCCTAGA TTTGACAAGG 
ACACAAGCAT CTCAGGAGAC GCTCGCCTCT ACCCCAGGGG GTCCCACACT GGCTTCATGG 
CCCTCTCGGG AAAAGTGGCT GCTTACAGGT GTCACAAGCT CCACGGGTGT CCCCAGCCCC 
AGCAGCCTGG GAGTAGACAT GGAAGAGACA ACACCCTCAG GCACACAGGT AGCCCCCACC 
CCCACAATGA GGAGGGGCCG CTTTAAAGGG TTGAATGGTC GACACTTCCA GCAACAGGGC 
CCAGAAGACC AGCTTCTGGA GGCAGCAGAG GCCAGTGCCC AGCCTCCCAC CCTGGAAGTT 
ACTGCTGACC ACATGGGGCC TTCTGCAGCC ACAGAGGCCT TGGAGAGTGA CCAGAGCCAC 
AGTCCTTGGG CCATTCTGAC CAATGAAGTG GATGTGCCAG GGGCAGGCTC TCTTGGCAGC 
AGGAGTCTCC CAGAGTCCCG GAAGTGGTCC CCGTCGCTGA TTTCACCCAG TACTGTCCCG 
AGCACTGACA GTACTCCTGG CCTGAAGCCA GGGGCAGATG AGGCCCCTGG TGTGAAGTCA 
GCCATCCACC ACCCACCCTG GTTGCCCTCA GAACCCGCTG TCCCATCCTC CATTCCCTCA 
GAGGCCCTAA GTGCTGTCTC CCTACAGGCA TCCCCTGGTG ATGGCTCTCC AGACTTCCCC 
ATTGTAGCCA TGCTTCGAGC CCCCAAACTG TGGCTTCTGC CACACTCTAC ACTCGTCCCG 
AATGTGTCCC CAATCCCACT CTCCCCAGCT TCTCCACTCC CCTCCTCGGT CCCAGAAGAA 
CAGGCTGTCA GACCTGTCAG CTTTGGAGCA GAAGACCCCG AGACCCCATT TCAGACCACC 
ATGGCTGCCC CAGGTGAAGC CAGCCACGGA TCCCCTGAGG CAGACTCCAT AGAAATCGAG 
GGGATCAGCT CCATGCAGGC TACAAAGCAC CCCATCTCTG GCCCATGGGC TTCTTTGGAC 
TCCAGTAATG TGACAGTGAA TCCTGTCCCT TCTGATGCTG GCATCCTAGG GACTGAGTCT 
GGGGTCTTGG ACTTACCAGG GAGTCCCACA TCAGACGGAC AGGCCACTGT GGACAAGGTG 
CTGGCCACCT GGCTACCACT GCCTGGCCAC GGACTGGACA CTGGCTCCCA GTCCACACCC 
ATGGAAGCCC ATGGAGTAAC CATGAGTGTG GAACCTACAG TGGCTTTGGA AGGAGGTGCC 
ACCAAAGACC CAATGGAGGC CACCATGGAT GTGGTCCCCA GCACTGTTGA TGCCACTTCG 
GGGTCTGAAC CCAAAAGTTC CATTTCTAGC ACCCATGTGG TTGTGACTGC AGCTGGGGAC 
CAGGGCACAC CCACACTGAC CCCTACAAGC TCTGAAGGTC AGGTGGTGGC CCAGGAGTCA 
CTGGGAACCC TCACCAGTCT GCCTTCTCAT CCCTGGTCAT CTCTGGCCTC CAGCATGGAC 
GAAGTGGCCT CGGTTTCCTC AGGAGAACCC ACAGGGTTGT GGGACATCCC CAGCACTCTG 
ATACCTGTGT CCTTGGGCTT GGATGAATCA GACCTGAAGG TTGTGGCTGA GAGCCCAGGC 
TTGGAGGGCT TCTGGGAAGA GGTGGCCAGT GGGCAGGAAG ACCCCACGGA TCCCTGCGAG 
AACAACCCTT GTCTGCACGG GGGCACCTGC CGCACAAATG GCACCATGTA CGGCTGTAGT 
TGTGATCAGG GCTATGCTGG GGAGAATTGT GAAATTGACA TTGATGACTG CTTGTGCAGC 
CCTTGTGAGA ATGGGGGTAC CTGCATTGAT GAGGTGAATG GTTTCATCTG CCTCTGTCTC 
CCCAGCTATG GGGGCAACCT GTGCGAGAAG GACACAGAAG GATGCGACCG TGGCTGGCAC 
AAATTCCAGG GCCACTGCTA CCGCTACTTT GCTCATCGGC GGGCCTGGGA GGACGCAGAG 
AGAGACTGCA GGCGCCGAGC CGGCCACCTG ACAAGTGTCC ACTCCCCAGA AGAGCACAAG 
TTTATTAACA GTTTTGGACA CGAGAATTCA TGGATTGGCC TGAATGACAG GACAGTAGAG 
AGGGACTTCC AGTGGACAGA CAACACAGGA CTGCAATATG AGAACTGGAG AGAGAAGCAG 
CCGGATAATT TCTTCGCAGG TGGGGAGGAT TGTGTGGTGA TGGTGGCGCA TGAGAATGGA 
CGCTGGAATG ATGTCCCCTG TAACTACAAC CTCCCCTACG TCTGCAAGAA GGGTACAGTG 
CTGTGTGGGC CCCCTCCAGC AGTGGAGAAT GCCTCTCTTG TTGGTGTGCG CAAGGTCAAG 
TACAATGTCC ATGCCACTGT GCGATACCAG TGTGATGAAG GATTCTCCCA GCACCATGTG 
GCTACCATCC GATGCCGAAA CAATGGGAAG TGGGACCGGC CTCAGATTGT GTGCACCAAG 
CCCAGGCGGT CACATCGGAT GCGTCGACAC CACCACCATC CACACCGGCA TCACAAGCCA 
CGCAAGGAGC ACAGAAAACA CAAGAGACAC CCAGCGGAAG ACTGGGAGAA AGATGAAGGG 
GATTTCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113841.2
CDS	77..3868
Translation	MGAESVWASGLLVLWLLLLVSGDQDTQDTTTTEKGLHMLKSGSGPIQAALAELVALPCFFTLQPRQSPLGDIPRIKWTKVQTASGQRQDLPILVAKDNVVRVAKGWQGRVSLPAYPRHRANATLLLGPLRASDSGLYRCQVVKGIEDEQDLVTLEVTGVVFHYRAARDRYALTFAEAQEACHLSSATIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRSYGRRDPQELYDVYCFARELGGEVFYVGPARRLTLAGARALCQRQGAALASVGQLHLAWHEGLDQCDPGWLADGSVRYPIQTPRRRCGGSAPGVRTVYRFANRTGFPAPGARFDAYCFRAHHHTPQRGDSEIPSSGDEGEIVSAEGPPAPELKPRLGEQEVITPDFQEPLVSSGEDEPLDLTRTQASQETLASTPGGPTLASWPSREKWLLTGVTSSTGVPSPSSLGVDMEETTPSGTQVAPTPTMRRGRFKGLNGRHFQQQGPEDQLLEAAEASAQPPTLEVTADHMGPSAATEALESDQSHSPWAILTNEVDVPGAGSLGSRSLPESRKWSPSLISPSTVPSTDSTPGLKPGADEAPGVKSAIHHPPWLPSEPAVPSSIPSEALSAVSLQASPGDGSPDFPIVAMLRAPKLWLLPHSTLVPNVSPIPLSPASPLPSSVPEEQAVRPVSFGAEDPETPFQTTMAAPGEASHGSPEADSIEIEGISSMQATKHPISGPWASLDSSNVTVNPVPSDAGILGTESGVLDLPGSPTSDGQATVDKVLATWLPLPGHGLDTGSQSTPMEAHGVTMSVEPTVALEGGATKDPMEATMDVVPSTVDATSGSEPKSSISSTHVVVTAAGDQGTPTLTPTSSEGQVVAQESLGTLTSLPSHPWSSLASSMDEVASVSSGEPTGLWDIPSTLIPVSLGLDESDLKVVAESPGLEGFWEEVASGQEDPTDPCENNPCLHGGTCRTNGTMYGCSCDQGYAGENCEIDIDDCLCSPCENGGTCIDEVNGFICLCLPSYGGNLCEKDTEGCDRGWHKFQGHCYRYFAHRRAWEDAERDCRRRAGHLTSVHSPEEHKFINSFGHENSWIGLNDRTVERDFQWTDNTGLQYENWREKQPDNFFAGGEDCVVMVAHENGRWNDVPCNYNLPYVCKKGTVLCGPPPAVENASLVGVRKVKYNVHATVRYQCDEGFSQHHVATIRCRNNGKWDRPQIVCTKPRRSHRMRRHHHHPHRHHKPRKEHRKHKRHPAEDWEKDEGDFC

Target ORF information:

RefSeq Version	NM_031653.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurocan (Ncan), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031653.2

ORF Insert Sequence:

ATGGGGGCCG AATCTGTCTG GGCCTCAGGC CTCCTGGTGC TGTGGCTGCT TCTCCTAGTG 
TCTGGGGATC AGGACACACA GGACACCACC ACCACGGAAA AGGGGCTTCA CATGCTGAAG 
TCGGGGTCAG GACCCATCCA GGCTGCTTTG GCAGAGTTAG TGGCCCTGCC CTGCTTCTTT 
ACCCTTCAAC CACGGCAAAG CCCCCTGGGA GACATTCCTC GGATCAAGTG GACGAAAGTT 
CAGACTGCAT CAGGCCAGCG ACAGGATTTG CCAATCTTGG TGGCCAAAGA CAACGTGGTG 
CGTGTGGCCA AGGGCTGGCA GGGACGGGTG TCATTGCCTG CCTATCCCCG GCACAGAGCC 
AATGCTACAC TTCTGTTGGG GCCACTGCGA GCCAGTGACT CTGGGCTGTA TCGCTGCCAA 
GTGGTGAAGG GTATCGAGGA TGAGCAGGAC CTGGTAACCC TGGAAGTGAC AGGCGTCGTG 
TTCCATTATC GGGCGGCCCG GGACCGCTAT GCGTTGACCT TCGCTGAGGC CCAGGAGGCT 
TGTCACCTGA GCTCCGCTAC CATTGCGGCT CCAAGGCACC TGCAGGCTGC GTTCGAAGAT 
GGCTTTGACA ACTGCGATGC GGGCTGGCTC TCAGACCGCA CGGTCCGGTA CCCGATCACT 
CAGTCGCGTC CCGGTTGCTA TGGTGATCGC AGCAGCCTGC CAGGTGTCCG GAGCTACGGG 
AGACGCGACC CGCAGGAACT CTACGATGTC TACTGCTTTG CCCGCGAGCT AGGGGGTGAA 
GTCTTTTACG TGGGCCCCGC CCGCCGACTG ACCCTGGCGG GGGCGCGGGC ACTGTGTCAG 
CGTCAGGGTG CAGCGCTGGC CTCCGTGGGA CAGTTGCACC TGGCCTGGCA CGAGGGCCTG 
GACCAGTGCG ACCCGGGCTG GCTGGCAGAC GGCAGCGTGC GCTACCCGAT CCAGACTCCA 
CGGCGGCGTT GCGGGGGCTC CGCTCCAGGT GTGCGCACAG TGTACCGCTT CGCCAACCGC 
ACTGGCTTTC CTGCGCCAGG AGCCCGCTTC GACGCCTACT GCTTCCGAGC TCATCACCAT 
ACACCACAAC GTGGAGACTC CGAGATCCCC TCATCTGGAG ATGAGGGGGA GATTGTGTCA 
GCAGAGGGGC CGCCAGCCCC AGAACTAAAG CCCAGATTGG GGGAGCAGGA GGTGATAACA 
CCTGACTTCC AGGAACCTCT CGTATCCAGT GGAGAAGATG AACCCCTAGA TTTGACAAGG 
ACACAAGCAT CTCAGGAGAC GCTCGCCTCT ACCCCAGGGG GTCCCACACT GGCTTCATGG 
CCCTCTCGGG AAAAGTGGCT GCTTACAGGT GTCACAAGCT CCACGGGTGT CCCCAGCCCC 
AGCAGCCTGG GAGTAGACAT GGAAGAGACA ACACCCTCAG GCACACAGGT AGCCCCCACC 
CCCACAATGA GGAGGGGCCG CTTTAAAGGG TTGAATGGTC GACACTTCCA GCAACAGGGC 
CCAGAAGACC AGCTTCTGGA GGCAGCAGAG GCCAGTGCCC AGCCTCCCAC CCTGGAAGTT 
ACTGCTGACC ACATGGGGCC TTCTGCAGCC ACAGAGGCCT TGGAGAGTGA CCAGAGCCAC 
AGTCCTTGGG CCATTCTGAC CAATGAAGTG GATGTGCCAG GGGCAGGCTC TCTTGGCAGC 
AGGAGTCTCC CAGAGTCCCG GAAGTGGTCC CCGTCGCTGA TTTCACCCAG TACTGTCCCG 
AGCACTGACA GTACTCCTGG CCTGAAGCCA GGGGCAGATG AGGCCCCTGG TGTGAAGTCA 
GCCATCCACC ACCCACCCTG GTTGCCCTCA GAACCCGCTG TCCCATCCTC CATTCCCTCA 
GAGGCCCTAA GTGCTGTCTC CCTACAGGCA TCCCCTGGTG ATGGCTCTCC AGACTTCCCC 
ATTGTAGCCA TGCTTCGAGC CCCCAAACTG TGGCTTCTGC CACACTCTAC ACTCGTCCCG 
AATGTGTCCC CAATCCCACT CTCCCCAGCT TCTCCACTCC CCTCCTCGGT CCCAGAAGAA 
CAGGCTGTCA GACCTGTCAG CTTTGGAGCA GAAGACCCCG AGACCCCATT TCAGACCACC 
ATGGCTGCCC CAGGTGAAGC CAGCCACGGA TCCCCTGAGG CAGACTCCAT AGAAATCGAG 
GGGATCAGCT CCATGCAGGC TACAAAGCAC CCCATCTCTG GCCCATGGGC TTCTTTGGAC 
TCCAGTAATG TGACAGTGAA TCCTGTCCCT TCTGATGCTG GCATCCTAGG GACTGAGTCT 
GGGGTCTTGG ACTTACCAGG GAGTCCCACA TCAGACGGAC AGGCCACTGT GGACAAGGTG 
CTGGCCACCT GGCTACCACT GCCTGGCCAC GGACTGGACA CTGGCTCCCA GTCCACACCC 
ATGGAAGCCC ATGGAGTAAC CATGAGTGTG GAACCTACAG TGGCTTTGGA AGGAGGTGCC 
ACCAAAGACC CAATGGAGGC CACCATGGAT GTGGTCCCCA GCACTGTTGA TGCCACTTCG 
GGGTCTGAAC CCAAAAGTTC CATTTCTAGC ACCCATGTGG TTGTGACTGC AGCTGGGGAC 
CAGGGCACAC CCACACTGAC CCCTACAAGC TCTGAAGGTC AGGTGGTGGC CCAGGAGTCA 
CTGGGAACCC TCACCAGTCT GCCTTCTCAT CCCTGGTCAT CTCTGGCCTC CAGCATGGAC 
GAAGTGGCCT CGGTTTCCTC AGGAGAACCC ACAGGGTTGT GGGACATCCC CAGCACTCTG 
ATACCTGTGT CCTTGGGCTT GGATGAATCA GACCTGAAGG TTGTGGCTGA GAGCCCAGGC 
TTGGAGGGCT TCTGGGAAGA GGTGGCCAGT GGGCAGGAAG ACCCCACGGA TCCCTGCGAG 
AACAACCCTT GTCTGCACGG GGGCACCTGC CGCACAAATG GCACCATGTA CGGCTGTAGT 
TGTGATCAGG GCTATGCTGG GGAGAATTGT GAAATTGACA TTGATGACTG CTTGTGCAGC 
CCTTGTGAGA ATGGGGGTAC CTGCATTGAT GAGGTGAATG GTTTCATCTG CCTCTGTCTC 
CCCAGCTATG GGGGCAACCT GTGCGAGAAG GACACAGAAG GATGCGACCG TGGCTGGCAC 
AAATTCCAGG GCCACTGCTA CCGCTACTTT GCTCATCGGC GGGCCTGGGA GGACGCAGAG 
AGAGACTGCA GGCGCCGAGC CGGCCACCTG ACAAGTGTCC ACTCCCCAGA AGAGCACAAG 
TTTATTAACA GTTTTGGACA CGAGAATTCA TGGATTGGCC TGAATGACAG GACAGTAGAG 
AGGGACTTCC AGTGGACAGA CAACACAGGA CTGCAATATG AGAACTGGAG AGAGAAGCAG 
CCGGATAATT TCTTCGCAGG TGGGGAGGAT TGTGTGGTGA TGGTGGCGCA TGAGAATGGA 
CGCTGGAATG ATGTCCCCTG TAACTACAAC CTCCCCTACG TCTGCAAGAA GGGTACAGTG 
CTGTGTGGGC CCCCTCCAGC AGTGGAGAAT GCCTCTCTTG TTGGTGTGCG CAAGGTCAAG 
TACAATGTCC ATGCCACTGT GCGATACCAG TGTGATGAAG GATTCTCCCA GCACCATGTG 
GCTACCATCC GATGCCGAAA CAATGGGAAG TGGGACCGGC CTCAGATTGT GTGCACCAAG 
CCCAGGCGGT CACATCGGAT GCGTCGACAC CACCACCATC CACACCGGCA TCACAAGCCA 
CGCAAGGAGC ACAGAAAACA CAAGAGACAC CCAGCGGAAG ACTGGGAGAA AGATGAAGGG 
GATTTCTGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Olfactory ensheathing cells (OECs) degrade neurocan in injured spinal cord by secreting matrix metalloproteinase-2 in a rat contusion model.
The Japanese journal of veterinary research62(4)151-62(2014 Nov)
Yui S,Fujita N,Chung CS,Morita M,Nishimura R

Upregulation of CSPG3 accompanies neuronal progenitor proliferation and migration in EAE.
Journal of molecular neuroscience : MN43(3)531-40(2011 Mar)
Sajad M,Zargan J,Chawla R,Umar S,Khan HA

Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain.
Glia56(16)1747-54(2008 Dec)
Shen LH,Li Y,Gao Q,Savant-Bhonsale S,Chopp M

Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum.
Molecular & cellular proteomics : MCP5(6)1158-70(2006 Jun)
Cheng D,Hoogenraad CC,Rush J,Ramm E,Schlager MA,Duong DM,Xu P,Wijayawardana SR,Hanfelt J,Nakagawa T,Sheng M,Peng J

Composition of perineuronal net extracellular matrix in rat brain: a different disaccharide composition for the net-associated proteoglycans.
The Journal of biological chemistry281(26)17789-800(2006 Jun)
Deepa SS,Carulli D,Galtrey C,Rhodes K,Fukuda J,Mikami T,Sugahara K,Fawcett JW

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Ncan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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