Fxyd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fxyd1
Entrez Gene ID	58971
Full Name	FXYD domain-containing ion transport regulator 1
Synonyms	Plm
General protein information	Preferred Names FXYD domain-containing ion transport regulator 1 Names phospholemman sodium/potassium-transporting ATPase subunit FXYD1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589629.1	XP_017445118.1	phospholemman isoform X1
XM_006228839.3	XP_006228901.1	phospholemman isoform X3
XM_017589631.1	XP_017445120.1	phospholemman isoform X3
XM_008759184.2	XP_008757406.1	phospholemman isoform X3
NM_031648.2	NP_113836.2	phospholemman precursor
XM_017589630.1	XP_017445119.1	phospholemman isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FXYD1	NP_001069878.1
Xenopus tropicalis (tropical clawed frog)	fxyd1	NP_001011320.1
Pan troglodytes (chimpanzee)	FXYD1	XP_003316318.2
Rattus norvegicus (Norway rat)	Fxyd1	NP_113836.2
Homo sapiens (human)	FXYD1	NP_001265646.1
Canis lupus familiaris (dog)	FXYD1	NP_001182071.1
Mus musculus (house mouse)	Fxyd1	NP_919302.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman.
Hafver TL, Hodne K, Wanichawan P, Aronsen JM, Dalhus B, Lunde PK, Lunde M, Martinsen M, Enger UH, Fuller W, Sjaastad I, Louch WE, Sejersted OM, Carlson CR
The Journal of biological chemistry291(9)4561-79(2016 Feb)

Overexpression of the Na+/K+ ATPase ?2 but not ?1 isoform attenuates pathological cardiac hypertrophy and remodeling.
Correll RN, Eder P, Burr AR, Despa S, Davis J, Bers DM, Molkentin JD
Circulation research114(2)249-256(2014 Jan)

A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle.
Wypijewski KJ, Howie J, Reilly L, Tulloch LB, Aughton KL, McLatchie LM, Shattock MJ, Calaghan SC, Fuller W
The Journal of biological chemistry288(19)13808-20(2013 May)

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.
Chibalin AV, Heiny JA, Benziane B, Prokofiev AV, Vasiliev AV, Kravtsova VV, Krivoi II
PloS one7(3)e33719(2012)

The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation.
Tulloch LB, Howie J, Wypijewski KJ, Wilson CR, Bernard WG, Shattock MJ, Fuller W
The Journal of biological chemistry286(41)36020-31(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest Ncx1 (sodium-calcium exchanger 1) is substrate-specifying Pp1c (protein phosphatase 1c) regulator protein, regulating Ncx1 activity via Ser-68-Plm dephosphorylation in cardiomyocytes; Plm/Ncx1/Pp1c complex is up-regulated in heart failure.
Title: Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman.

PLM exists as a pump-inhibiting monomer and an unassociated oligomer.
Title: A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle.

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.
Title: Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.

role of protein phosphatase-1 on phospholemman phosphorylation and Na/K-ATPase activity in cardiomyocytes
Title: Phospholemman-dependent regulation of the cardiac Na/K-ATPase activity is modulated by inhibitor-1 sensitive type-1 phosphatase.

palmitoylation of PLM controls its turnover, and palmitoylated PLM inhibits the sodium pump.
Title: The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation.

The following Fxyd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fxyd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08368	NM_031648.2 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa22615	XM_017589630.1 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa22616	XM_017589631.1 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa22616	XM_006228839.3 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa22616	XM_008759184.2 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa56805	XM_017589629.1 Latest version!	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fxyd1 ( NM_031648.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113836.2 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), mRNA.

Price & Availability↑

CloneID	ORa08368
Clone ID Related Accession (Same CDS sequence)	NM_031648.2
Accession Version	NM_031648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB712570.1 and FM109156.1. On May 10, 2012 this sequence version replaced NM_031648.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ211349.1, CK476741.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGCAA TGGCATCTCC CGGCCACATC CTGATTGTCT GTGTGTGTCT CCTCTCCATG 
GCCAGTGCAG AAGCTCCGCA GGAACCAGAT CCATTCACCT ACGATTACCA CACCCTGCGG 
ATCGGCGGCC TCACTATCGC TGGGATCCTC TTCATCTTGG GCATCCTTAT CATCCTTAGC 
AAAAGATGCC GGTGCAAATT CAACCAACAG CAGAGAACTG GGGAACCCGA CGAAGAGGAG 
GGAACTTTCC GCAGCTCCAT CCGCCGTCTG TCCACCCGCA GGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113836.2
CDS	78..365
Translation	MGAMASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	NM_031648.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031648.2

ORF Insert Sequence:

ATGGGGGCAA TGGCATCTCC CGGCCACATC CTGATTGTCT GTGTGTGTCT CCTCTCCATG 
GCCAGTGCAG AAGCTCCGCA GGAACCAGAT CCATTCACCT ACGATTACCA CACCCTGCGG 
ATCGGCGGCC TCACTATCGC TGGGATCCTC TTCATCTTGG GCATCCTTAT CATCCTTAGC 
AAAAGATGCC GGTGCAAATT CAACCAACAG CAGAGAACTG GGGAACCCGA CGAAGAGGAG 
GGAACTTTCC GCAGCTCCAT CCGCCGTCTG TCCACCCGCA GGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd1 ( XM_017589630.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445119.1 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22615
Clone ID Related Accession (Same CDS sequence)	XM_017589630.1
Accession Version	XM_017589630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGTT TGTCTCACAG CATTCCACCT GTGTCCGGAG ATAGCTTGCT TCCCTTTCAA 
GGGGCAATGG CATCTCCCGG CCACATCCTG ATTGTCTGTG TGTGTCTCCT CTCCATGGCC 
AGTGCAGAAG CTCCGCAGGA ACCAGATCCA TTCACCTACG ATTACCACAC CCTGCGGATC 
GGCGGCCTCA CTATCGCTGG GATCCTCTTC ATCTTGGGCA TCCTTATCAT CCTTAGCAAA 
AGATGCCGGT GCAAATTCAA CCAACAGCAG AGAACTGGGG AACCCGACGA AGAGGAGGGA 
ACTTTCCGCA GCTCCATCCG CCGTCTGTCC ACCCGCAGGC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445119.1
CDS	67..411
Translation	MSCLSHSIPPVSGDSLLPFQGAMASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	XM_017589630.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589630.1

ORF Insert Sequence:

ATGTCCTGTT TGTCTCACAG CATTCCACCT GTGTCCGGAG ATAGCTTGCT TCCCTTTCAA 
GGGGCAATGG CATCTCCCGG CCACATCCTG ATTGTCTGTG TGTGTCTCCT CTCCATGGCC 
AGTGCAGAAG CTCCGCAGGA ACCAGATCCA TTCACCTACG ATTACCACAC CCTGCGGATC 
GGCGGCCTCA CTATCGCTGG GATCCTCTTC ATCTTGGGCA TCCTTATCAT CCTTAGCAAA 
AGATGCCGGT GCAAATTCAA CCAACAGCAG AGAACTGGGG AACCCGACGA AGAGGAGGGA 
ACTTTCCGCA GCTCCATCCG CCGTCTGTCC ACCCGCAGGC GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd1 ( XM_006228839.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228901.1 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22616
Clone ID Related Accession (Same CDS sequence)	XM_006228839.3 , XM_017589631.1 , XM_008759184.2
Accession Version	XM_006228839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228901.1
CDS	88..366
Translation	MASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	XM_006228839.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228839.3

ORF Insert Sequence:

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd1 ( XM_017589631.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445120.1 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa22616
Clone ID Related Accession (Same CDS sequence)	XM_006228839.3 , XM_017589631.1 , XM_008759184.2
Accession Version	XM_017589631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445120.1
CDS	158..436
Translation	MASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	XM_017589631.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589631.1

ORF Insert Sequence:

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd1 ( XM_008759184.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757406.1 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa22616
Clone ID Related Accession (Same CDS sequence)	XM_006228839.3 , XM_017589631.1 , XM_008759184.2
Accession Version	XM_008759184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757406.1
CDS	234..512
Translation	MASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	XM_008759184.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759184.2

ORF Insert Sequence:

ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fxyd1 ( XM_017589629.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445118.1 Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56805
Clone ID Related Accession (Same CDS sequence)	XM_017589629.1
Accession Version	XM_017589629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phospholemman isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGGA GATATTTATA CCCAGGGTCA GGGAGAGAGC GGGCAGGCGG CCGAGGGCAG 
GAGAACTGGG ACTGCCTGAG TACGGAGAGA CCACTGGCTG AGGTGTGTAG GGGCAGGGCA 
ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445118.1
CDS	81..479
Translation	MGGRYLYPGSGRERAGGRGQENWDCLSTERPLAEVCRGRAMASPGHILIVCVCLLSMASAEAPQEPDPFTYDYHTLRIGGLTIAGILFILGILIILSKRCRCKFNQQQRTGEPDEEEGTFRSSIRRLSTRRR

Target ORF information:

RefSeq Version	XM_017589629.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus FXYD domain-containing ion transport regulator 1 (Fxyd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589629.1

ORF Insert Sequence:

ATGGGTGGGA GATATTTATA CCCAGGGTCA GGGAGAGAGC GGGCAGGCGG CCGAGGGCAG 
GAGAACTGGG ACTGCCTGAG TACGGAGAGA CCACTGGCTG AGGTGTGTAG GGGCAGGGCA 
ATGGCATCTC CCGGCCACAT CCTGATTGTC TGTGTGTGTC TCCTCTCCAT GGCCAGTGCA 
GAAGCTCCGC AGGAACCAGA TCCATTCACC TACGATTACC ACACCCTGCG GATCGGCGGC 
CTCACTATCG CTGGGATCCT CTTCATCTTG GGCATCCTTA TCATCCTTAG CAAAAGATGC 
CGGTGCAAAT TCAACCAACA GCAGAGAACT GGGGAACCCG ACGAAGAGGA GGGAACTTTC 
CGCAGCTCCA TCCGCCGTCT GTCCACCCGC AGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman.
The Journal of biological chemistry291(9)4561-79(2016 Feb)
Hafver TL,Hodne K,Wanichawan P,Aronsen JM,Dalhus B,Lunde PK,Lunde M,Martinsen M,Enger UH,Fuller W,Sjaastad I,Louch WE,Sejersted OM,Carlson CR

Overexpression of the Na+/K+ ATPase ?2 but not ?1 isoform attenuates pathological cardiac hypertrophy and remodeling.
Circulation research114(2)249-256(2014 Jan)
Correll RN,Eder P,Burr AR,Despa S,Davis J,Bers DM,Molkentin JD

A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle.
The Journal of biological chemistry288(19)13808-20(2013 May)
Wypijewski KJ,Howie J,Reilly L,Tulloch LB,Aughton KL,McLatchie LM,Shattock MJ,Calaghan SC,Fuller W

Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.
PloS one7(3)e33719(2012)
Chibalin AV,Heiny JA,Benziane B,Prokofiev AV,Vasiliev AV,Kravtsova VV,Krivoi II

The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation.
The Journal of biological chemistry286(41)36020-31(2011 Oct)
Tulloch LB,Howie J,Wypijewski KJ,Wilson CR,Bernard WG,Shattock MJ,Fuller W

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Fxyd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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