Txnrd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Txnrd1
Entrez Gene ID	58819
Full Name	thioredoxin reductase 1
Synonyms	Tr
General protein information	Preferred Names thioredoxin reductase 1 Names thioredoxin reductase 1, cytoplasmic NADPH-dependent thioredoxin reductase selenoprotein oxidoreductase thioredoxin reductase TR1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, Jun 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001351981.1	NP_001338910.1	thioredoxin reductase 1, cytoplasmic isoform 1
NM_001351983.1	NP_001338912.1	thioredoxin reductase 1, cytoplasmic isoform 1
NM_031614.3	NP_113802.2	thioredoxin reductase 1, cytoplasmic isoform 1
NM_031614.2	NP_113802.2	thioredoxin reductase 1, cytoplasmic
NM_001351984.1	NP_001338913.1	thioredoxin reductase 1, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TXNRD1	NP_001116141.1
	TXNRD1	XP_002798903.1
Mus musculus (house mouse)	Txnrd1	NP_001035988.1
Arabidopsis thaliana (thale cress)	GR1	NP_001118688.1
Xenopus tropicalis (tropical clawed frog)	txnrd1	NP_001243400.1
Canis lupus familiaris (dog)	TXNRD1	NP_001116145.1
Bos taurus (cattle)	TXNRD1	NP_777050.1
Homo sapiens (human)	TXNRD1	NP_001087240.1
Rattus norvegicus (Norway rat)	Txnrd1	NP_113802.2
Gallus gallus (chicken)	TXNRD1	NP_001025933.2
Oryza sativa (rice)	Os02g0813500	NP_001048485.1

Gene Ontology
Bibliography

Related articles in PubMed

Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H
Dagnell M, Pace PE, Cheng Q, Frijhoff J, ?stman A, Arn?r ESJ, Hampton MB, Winterbourn CC
The Journal of biological chemistry292(35)14371-14380(2017 09)

Brevetoxin-2, is a unique inhibitor of the C-terminal redox center of mammalian thioredoxin reductase-1.
Chen W, Tuladhar A, Rolle S, Lai Y, Rodriguez Del Rey F, Zavala CE, Liu Y, Rein KS
Toxicology and applied pharmacology32958-66(2017 08)

Reduction of Tetrathionate by Mammalian Thioredoxin Reductase.
Narayan V, Kudva AK, Prabhu KS
Biochemistry54(33)5121-4(2015 Aug)

The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death.
Xu J, Eriksson SE, Cebula M, Sandalova T, Hedstr?m E, Pader I, Cheng Q, Myers CR, Antholine WE, Nagy P, Hellman U, Selivanova G, Lindqvist Y, Arn?r ES
Cell death & disease6e1616(2015 Jan)

Thioredoxin/thioredoxin reductase system involvement in cerebellar granule cell apoptosis.
Bobba A, Casalino E, Petragallo VA, Atlante A
Apoptosis : an international journal on programmed cell death19(10)1497-508(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest TrxR1 and NADPH directly protect PTP1B from inactivation by oxidation; this protection is independent of TRX1 and PRX2; this protection is blocked by auranofin (an inhibitor of TrxR1 and requires an intact selenocysteine residue in TrxR1. (TrxR1 = thioredoxin reductase 1; PTP1B = protein tyrosine phosphatase, non-receptor type 1; TRX1 = thioredoxin-1; PRX2 = paired related homeobox 2 protein)
Title: Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H

Inhibition of thioredoxin reductase-1 by brevetoxin-2 is via the formation of a Michael adduct between selenocysteine and the alpha, beta-unsaturated aldehyde moiety of the toxin.
Title: Brevetoxin-2, is a unique inhibitor of the C-terminal redox center of mammalian thioredoxin reductase-1.

rat liver selenoenzyme thioredoxin reductase (Txnrd1, TR1) efficiently reduces tetrathionate
Title: Reduction of Tetrathionate by Mammalian Thioredoxin Reductase.

Trp114 of TrxR1 serves a function reminiscent of an irreversible sensor for excessive oxidation.
Title: The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death.

Active TrxR1 and TrxR2 are required for cerebellar granule cell survival.
Title: Thioredoxin/thioredoxin reductase system involvement in cerebellar granule cell apoptosis.

The following Txnrd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Txnrd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12405	NM_001351981.1 Latest version!	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa12405	NM_031614.3 Latest version!	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa12405	NM_001351983.1 Latest version!	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa63271	NM_001351984.1 Latest version!	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
ORa12405	NM_031614.2	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Txnrd1 ( NM_001351981.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001338910.1 Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12405
Clone ID Related Accession (Same CDS sequence)	NM_001351981.1 , NM_001351983.1 , NM_031614.3 , NM_031614.2
Accession Version	NM_001351981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase 1, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB579100.1, AF220761.1, BC085726.1 and BF415095.1. Transcript Variant: This variant (2) lacks a 5' non-coding exon; therefore, has a shorter 5' UTR compared to variant 1. Variants 1-3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF108213.1, AF220760.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10333487, 10769168 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338910.1
CDS	146..1645
Translation	MNDSKDAPKSYDFDLIIIGGGSGGLAAAKEAAKFDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKLEDTVKHDWEKMTESVQNHIGSLNWGYRVALREKKVVYENAYGKFIGPHKIMATNNKGKEKVYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLKVTAKSTNSEETIEDEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKVVCNLKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G

Target ORF information:

RefSeq Version	NM_001351981.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351981.1

ORF Insert Sequence:

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Txnrd1 ( NM_001351983.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001338912.1 Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa12405
Clone ID Related Accession (Same CDS sequence)	NM_001351981.1 , NM_001351983.1 , NM_031614.3 , NM_031614.2
Accession Version	NM_001351983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase 1, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FQ090798.1, FQ225805.1, BC085726.1 and BF415095.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-3 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF108213.1, AF220760.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10333487, 10769168 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338912.1
CDS	146..1645
Translation	MNDSKDAPKSYDFDLIIIGGGSGGLAAAKEAAKFDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKLEDTVKHDWEKMTESVQNHIGSLNWGYRVALREKKVVYENAYGKFIGPHKIMATNNKGKEKVYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLKVTAKSTNSEETIEDEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKVVCNLKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G

Target ORF information:

RefSeq Version	NM_001351983.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351983.1

ORF Insert Sequence:

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Txnrd1 ( NM_031614.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113802.2 Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12405
Clone ID Related Accession (Same CDS sequence)	NM_001351981.1 , NM_001351983.1 , NM_031614.3 , NM_031614.2
Accession Version	NM_031614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase 1, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB579100.1, BC085726.1 and BF415095.1. On Jun 2, 2017 this sequence version replaced NM_031614.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. Variants 1-3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF108213.1, AF220760.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10333487, 10769168 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113802.2
CDS	254..1753
Translation	MNDSKDAPKSYDFDLIIIGGGSGGLAAAKEAAKFDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKLEDTVKHDWEKMTESVQNHIGSLNWGYRVALREKKVVYENAYGKFIGPHKIMATNNKGKEKVYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLKVTAKSTNSEETIEDEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKVVCNLKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G

Target ORF information:

RefSeq Version	NM_031614.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031614.3

ORF Insert Sequence:

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Txnrd1 ( NM_031614.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113802.2 Rattus norvegicus thioredoxin reductase 1 (Txnrd1), mRNA.

Price & Availability↑

CloneID	ORa12405
Clone ID Related Accession (Same CDS sequence)	NM_001351981.1 , NM_001351983.1 , NM_031614.3 , NM_031614.2
Accession Version	NM_031614.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-15
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase 1, cytoplasmic
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC085726.1 and AA955679.1. On Oct 27, 2005 this sequence version replaced NM_031614.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF108213.1, AF220760.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113802.2
CDS	238..1737
Translation	MNDSKDAPKSYDFDLIIIGGGSGGLAAAKEAAKFDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKLEDTVKHDWEKMTESVQNHIGSLNWGYRVALREKKVVYENAYGKFIGPHKIMATNNKGKEKVYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLKVTAKSTNSEETIEDEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKVVCNLKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G

Target ORF information:

RefSeq Version	NM_031614.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031614.2

ORF Insert Sequence:

ATGAATGACT CTAAAGATGC CCCTAAGTCC TATGACTTCG ACCTGATCAT CATTGGAGGA 
GGCTCGGGAG GCCTGGCGGC AGCTAAGGAG GCAGCCAAAT TTGACAAGAA GGTGATGGTC 
TTGGACTTCG TCACACCAAC TCCTCTCGGA ACGAGATGGG GTCTCGGGGG AACGTGTGTG 
AACGTGGGCT GCATACCTAA AAAACTGATG CACCAGGCGG CTCTGTTAGG ACAAGCTCTG 
AAAGACTCAC GCAACTATGG CTGGAAACTC GAGGACACAG TTAAGCATGA CTGGGAGAAA 
ATGACAGAAT CTGTGCAGAA TCATATCGGC TCGCTGAACT GGGGCTACCG AGTAGCTCTC 
CGGGAGAAGA AGGTTGTCTA TGAGAATGCT TACGGGAAAT TCATTGGTCC TCACAAAATT 
ATGGCAACAA ATAACAAAGG TAAAGAAAAA GTTTACTCAG CAGAGCGGTT CCTCATTGCC 
ACCGGTGAAA GGCCGCGCTA CCTGGGCATC CCTGGAGACA AAGAGTACTG CATCAGCAGT 
GACGATCTTT TCTCCTTGCC TTACTGCCCG GGGAAGACCC TAGTGGTTGG CGCGTCCTAT 
GTCGCCTTGG AATGTGCAGG ATTCCTGGCT GGTATCGGCC TCGACGTCAC TGTAATGGTG 
CGGTCCATTC TCCTTAGAGG ATTTGACCAG GACATGGCCA ACAAAATTGG TGAACACATG 
GAAGAGCATG GTATCAAGTT TATCAGGCAG TTCGTGCCGA CGAAAATTGA ACAGATTGAA 
GCAGGGACAC CAGGCCGACT CAAGGTGACC GCTAAGTCCA CAAACAGTGA GGAGACCATA 
GAAGACGAAT TTAACACAGT GTTGCTTGCA GTAGGAAGAG ATTCTTGTAC AAGAACTATT 
GGCTTAGAGA CCGTGGGCGT GAAGATCAAT GAAAAGACCG GGAAGATACC TGTCACGGAT 
GAGGAGCAGA CCAATGTGCC TTACATCTAC GCCATTGGTG ACATTCTGGA GGGGAAGCTG 
GAGCTGACCC CCGTGGCCAT CCAGGCGGGG AGATTGCTGG CTCAGAGGCT GTATGGCGGC 
TCCACTGTCA AATGTGACTA TGACAATGTC CCAACGACTG TGTTTACTCC TTTGGAGTAT 
GGCTGCTGTG GCCTCTCTGA AGAAAAAGCT GTAGAGAAAT TTGGGGAAGA AAATATTGAA 
GTTTACCACA GTTTCTTCTG GCCATTGGAA TGGACAGTTC CATCCCGGGA TAACAACAAA 
TGTTATGCAA AAGTCGTCTG TAACCTTAAA GACAACGAAC GTGTCGTGGG CTTCCACGTA 
CTGGGTCCAA ATGCTGGAGA GGTGACGCAG GGCTTTGCAG CCGCACTCAA GTGCGGGCTG 
ACCAAGCAGC AGCTGGACAG CACCATTGGC ATCCACCCGG TCTGTGCAGA GATATTTACA 
ACGCTGTCGG TGACTAAGCG TTCTGGGGGA GACATCCTCC AGTCTGGCTG CTGAGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Txnrd1 ( NM_001351984.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001338913.1 Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ORa63271
Clone ID Related Accession (Same CDS sequence)	NM_001351984.1
Accession Version	NM_001351984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase 1, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC085726.1 and BF415095.1. Transcript Variant: This variant (4) represents use of an alternate upstream promoter, and contains additional exons at the 5' end compared to variant 1. The encoded isoform (2) has a longer and distinct N-terminus, resulting from translation initiation at an in-frame upstream start codon, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085726.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10333487, 10769168 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGTGTG CTTCCGTCAG GCCTCGCATC CACGCGGGAG GTGCGGGACG CCGACGCGGC 
GGGGCGAGAA GAGCCGGTGT TTTCATCCTC CTTGCTCATA GGGCGGCGGG GCCTTGCAGC 
GGCGCGGGCA AACGGAGAGG CCGCGGGAGG CGGCGAGCCG GCAGAAGGCG AGGGAGAGCG 
GAGGGCGGCC ATGGTCCAGC CCTGAAGCCA AACAAAAAAG GCCTACTTCG AAAGCTGTCA 
ACAATGAATG ACTCTAAAGA TGCCCCTAAG TCCTATGACT TCGACCTGAT CATCATTGGA 
GGAGGCTCGG GAGGCCTGGC GGCAGCTAAG GAGGCAGCCA AATTTGACAA GAAGGTGATG 
GTCTTGGACT TCGTCACACC AACTCCTCTC GGAACGAGAT GGGGTCTCGG GGGAACGTGT 
GTGAACGTGG GCTGCATACC TAAAAAACTG ATGCACCAGG CGGCTCTGTT AGGACAAGCT 
CTGAAAGACT CACGCAACTA TGGCTGGAAA CTCGAGGACA CAGTTAAGCA TGACTGGGAG 
AAAATGACAG AATCTGTGCA GAATCATATC GGCTCGCTGA ACTGGGGCTA CCGAGTAGCT 
CTCCGGGAGA AGAAGGTTGT CTATGAGAAT GCTTACGGGA AATTCATTGG TCCTCACAAA 
ATTATGGCAA CAAATAACAA AGGTAAAGAA AAAGTTTACT CAGCAGAGCG GTTCCTCATT 
GCCACCGGTG AAAGGCCGCG CTACCTGGGC ATCCCTGGAG ACAAAGAGTA CTGCATCAGC 
AGTGACGATC TTTTCTCCTT GCCTTACTGC CCGGGGAAGA CCCTAGTGGT TGGCGCGTCC 
TATGTCGCCT TGGAATGTGC AGGATTCCTG GCTGGTATCG GCCTCGACGT CACTGTAATG 
GTGCGGTCCA TTCTCCTTAG AGGATTTGAC CAGGACATGG CCAACAAAAT TGGTGAACAC 
ATGGAAGAGC ATGGTATCAA GTTTATCAGG CAGTTCGTGC CGACGAAAAT TGAACAGATT 
GAAGCAGGGA CACCAGGCCG ACTCAAGGTG ACCGCTAAGT CCACAAACAG TGAGGAGACC 
ATAGAAGACG AATTTAACAC AGTGTTGCTT GCAGTAGGAA GAGATTCTTG TACAAGAACT 
ATTGGCTTAG AGACCGTGGG CGTGAAGATC AATGAAAAGA CCGGGAAGAT ACCTGTCACG 
GATGAGGAGC AGACCAATGT GCCTTACATC TACGCCATTG GTGACATTCT GGAGGGGAAG 
CTGGAGCTGA CCCCCGTGGC CATCCAGGCG GGGAGATTGC TGGCTCAGAG GCTGTATGGC 
GGCTCCACTG TCAAATGTGA CTATGACAAT GTCCCAACGA CTGTGTTTAC TCCTTTGGAG 
TATGGCTGCT GTGGCCTCTC TGAAGAAAAA GCTGTAGAGA AATTTGGGGA AGAAAATATT 
GAAGTTTACC ACAGTTTCTT CTGGCCATTG GAATGGACAG TTCCATCCCG GGATAACAAC 
AAATGTTATG CAAAAGTCGT CTGTAACCTT AAAGACAACG AACGTGTCGT GGGCTTCCAC 
GTACTGGGTC CAAATGCTGG AGAGGTGACG CAGGGCTTTG CAGCCGCACT CAAGTGCGGG 
CTGACCAAGC AGCAGCTGGA CAGCACCATT GGCATCCACC CGGTCTGTGC AGAGATATTT 
ACAACGCTGT CGGTGACTAA GCGTTCTGGG GGAGACATCC TCCAGTCTGG CTGCTGAGGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338913.1
CDS	92..1834
Translation	MKCASVRPRIHAGGAGRRRGGARRAGVFILLAHRAAGPCSGAGKRRGRGRRRAGRRRGRAEGGHGPALKPNKKGLLRKLSTMNDSKDAPKSYDFDLIIIGGGSGGLAAAKEAAKFDKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKLEDTVKHDWEKMTESVQNHIGSLNWGYRVALREKKVVYENAYGKFIGPHKIMATNNKGKEKVYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLKVTAKSTNSEETIEDEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKVVCNLKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G

Target ORF information:

RefSeq Version	NM_001351984.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus thioredoxin reductase 1 (Txnrd1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351984.1

ORF Insert Sequence:

ATGAAGTGTG CTTCCGTCAG GCCTCGCATC CACGCGGGAG GTGCGGGACG CCGACGCGGC 
GGGGCGAGAA GAGCCGGTGT TTTCATCCTC CTTGCTCATA GGGCGGCGGG GCCTTGCAGC 
GGCGCGGGCA AACGGAGAGG CCGCGGGAGG CGGCGAGCCG GCAGAAGGCG AGGGAGAGCG 
GAGGGCGGCC ATGGTCCAGC CCTGAAGCCA AACAAAAAAG GCCTACTTCG AAAGCTGTCA 
ACAATGAATG ACTCTAAAGA TGCCCCTAAG TCCTATGACT TCGACCTGAT CATCATTGGA 
GGAGGCTCGG GAGGCCTGGC GGCAGCTAAG GAGGCAGCCA AATTTGACAA GAAGGTGATG 
GTCTTGGACT TCGTCACACC AACTCCTCTC GGAACGAGAT GGGGTCTCGG GGGAACGTGT 
GTGAACGTGG GCTGCATACC TAAAAAACTG ATGCACCAGG CGGCTCTGTT AGGACAAGCT 
CTGAAAGACT CACGCAACTA TGGCTGGAAA CTCGAGGACA CAGTTAAGCA TGACTGGGAG 
AAAATGACAG AATCTGTGCA GAATCATATC GGCTCGCTGA ACTGGGGCTA CCGAGTAGCT 
CTCCGGGAGA AGAAGGTTGT CTATGAGAAT GCTTACGGGA AATTCATTGG TCCTCACAAA 
ATTATGGCAA CAAATAACAA AGGTAAAGAA AAAGTTTACT CAGCAGAGCG GTTCCTCATT 
GCCACCGGTG AAAGGCCGCG CTACCTGGGC ATCCCTGGAG ACAAAGAGTA CTGCATCAGC 
AGTGACGATC TTTTCTCCTT GCCTTACTGC CCGGGGAAGA CCCTAGTGGT TGGCGCGTCC 
TATGTCGCCT TGGAATGTGC AGGATTCCTG GCTGGTATCG GCCTCGACGT CACTGTAATG 
GTGCGGTCCA TTCTCCTTAG AGGATTTGAC CAGGACATGG CCAACAAAAT TGGTGAACAC 
ATGGAAGAGC ATGGTATCAA GTTTATCAGG CAGTTCGTGC CGACGAAAAT TGAACAGATT 
GAAGCAGGGA CACCAGGCCG ACTCAAGGTG ACCGCTAAGT CCACAAACAG TGAGGAGACC 
ATAGAAGACG AATTTAACAC AGTGTTGCTT GCAGTAGGAA GAGATTCTTG TACAAGAACT 
ATTGGCTTAG AGACCGTGGG CGTGAAGATC AATGAAAAGA CCGGGAAGAT ACCTGTCACG 
GATGAGGAGC AGACCAATGT GCCTTACATC TACGCCATTG GTGACATTCT GGAGGGGAAG 
CTGGAGCTGA CCCCCGTGGC CATCCAGGCG GGGAGATTGC TGGCTCAGAG GCTGTATGGC 
GGCTCCACTG TCAAATGTGA CTATGACAAT GTCCCAACGA CTGTGTTTAC TCCTTTGGAG 
TATGGCTGCT GTGGCCTCTC TGAAGAAAAA GCTGTAGAGA AATTTGGGGA AGAAAATATT 
GAAGTTTACC ACAGTTTCTT CTGGCCATTG GAATGGACAG TTCCATCCCG GGATAACAAC 
AAATGTTATG CAAAAGTCGT CTGTAACCTT AAAGACAACG AACGTGTCGT GGGCTTCCAC 
GTACTGGGTC CAAATGCTGG AGAGGTGACG CAGGGCTTTG CAGCCGCACT CAAGTGCGGG 
CTGACCAAGC AGCAGCTGGA CAGCACCATT GGCATCCACC CGGTCTGTGC AGAGATATTT 
ACAACGCTGT CGGTGACTAA GCGTTCTGGG GGAGACATCC TCCAGTCTGG CTGCTGAGGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thioredoxin reductase 1 and NADPH directly protect protein tyrosine phosphatase 1B from inactivation during H
The Journal of biological chemistry292(35)14371-14380(2017 09)
Dagnell M,Pace PE,Cheng Q,Frijhoff J,?stman A,Arn?r ESJ,Hampton MB,Winterbourn CC

Brevetoxin-2, is a unique inhibitor of the C-terminal redox center of mammalian thioredoxin reductase-1.
Toxicology and applied pharmacology32958-66(2017 08)
Chen W,Tuladhar A,Rolle S,Lai Y,Rodriguez Del Rey F,Zavala CE,Liu Y,Rein KS

Reduction of Tetrathionate by Mammalian Thioredoxin Reductase.
Biochemistry54(33)5121-4(2015 Aug)
Narayan V,Kudva AK,Prabhu KS

The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death.
Cell death & disease6e1616(2015 Jan)
Xu J,Eriksson SE,Cebula M,Sandalova T,Hedstr?m E,Pader I,Cheng Q,Myers CR,Antholine WE,Nagy P,Hellman U,Selivanova G,Lindqvist Y,Arn?r ES

Thioredoxin/thioredoxin reductase system involvement in cerebellar granule cell apoptosis.
Apoptosis : an international journal on programmed cell death19(10)1497-508(2014 Oct)
Bobba A,Casalino E,Petragallo VA,Atlante A

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Txnrd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報