Nt5e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nt5e
Entrez Gene ID	58813
Full Name	5' nucleotidase, ecto
Synonyms	CD73,Nt5
General protein information	Preferred Names 5' nucleotidase, ecto Names 5'-nucleotidase 5'-NT ecto-5'-nucleotidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021576.2	NP_067587.2	5'-nucleotidase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NT5E	XP_518619.3
Gallus gallus (chicken)	NT5E	XP_004940454.1
Danio rerio (zebrafish)	nt5e	NP_957226.1
Drosophila melanogaster (fruit fly)	NT5E-2	NP_611218.1
Xenopus tropicalis (tropical clawed frog)	nt5e	XP_002933973.1
Canis lupus familiaris (dog)	NT5E	XP_532221.2
Rattus norvegicus (Norway rat)	Nt5e	NP_067587.2
Drosophila melanogaster (fruit fly)	veil	NP_611217.1
Homo sapiens (human)	NT5E	NP_002517.1
	NT5E	XP_001086989.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003629	XP_313394.4
Mus musculus (house mouse)	Nt5e	NP_035981.1
Magnaporthe oryzae (rice blast fungus)	MGG_12474	XP_003715781.1
Neurospora crassa	NCU09659	XP_958725.1

Gene Ontology
Bibliography

Related articles in PubMed

Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73
Suto EG, Mabuchi Y, Suzuki N, Suzuki K, Ogata Y, Taguchi M, Muneta T, Sekiya I, Akazawa C
Scientific reports7(1)4838(2017 Jul)

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.
Hesse J, Leberling S, Boden E, Friebe D, Schmidt T, Ding Z, Dieterich P, Deussen A, Roderigo C, Rose CR, Floss DM, Scheller J, Schrader J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3040-3053(2017 Jul)

Ecto-5'-nucleotidase (CD73) regulates peripheral chemoreceptor activity and cardiorespiratory responses to hypoxia.
Holmes AP, Ray CJ, Pearson SA, Coney AM, Kumar P
The Journal of physiology(2017 May)

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Maciel RM, Carvalho FB, Olabiyi AA, Schmatz R, Gutierres JM, Stefanello N, Zanini D, Rosa MM, Andrade CM, Rubin MA, Schetinger MR, Morsch VM, Danesi CC, Lopes STA
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Epicardium-derived cells from post-myocardial infarction hearts can generate adenosine from both extracellular ATP and NAD via the action of CD73.
Title: CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.

Quercetin improves memory and anxiogenic-like behavior in diabetic rats by preventing the decrease in Nt5e activity seen in streptozocin induced diabetes.
Title: Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.

Fluvastatin increases adenosine concentrations in the heart ventricles as a result of activation of PKC, mediated by stimulation of alpha1-adrenoceptors, through activation of ecto-5'-nucleotidase.
Title: Fluvastatin, an HMG-CoA reductase inhibitor, facilitate adenosine production in the rat hearts via activation of ecto-5'-nucleotidase.

Study supports that regulatory estradiol action at the activity and protein abundance of ecto-5'-nucleotidase is mediated by estrogen ERalpha and ERbeta in the hippocampal synaptosomes
Title: 17?-Estradiol upregulates ecto-5'-nucleotidase (CD73) in hippocampal synaptosomes of female rats through action mediated by estrogen receptor-? and -?.

The increase of CD73 activity is a key component in the production of high levels of adenosine and emerges as a new tool for the early diagnosis of tubular injury in diabetic kidney disease.
Title: Increased levels of adenosine and ecto 5'-nucleotidase (CD73) activity precede renal alterations in experimental diabetic rats.

The following Nt5e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nt5e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04842	NM_021576.2 Latest version!	Rattus norvegicus 5' nucleotidase, ecto (Nt5e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$335.30 $479.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nt5e ( NM_021576.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067587.2 Rattus norvegicus 5' nucleotidase, ecto (Nt5e), mRNA.

Price & Availability↑

CloneID	ORa04842
Clone ID Related Accession (Same CDS sequence)	NM_021576.2
Accession Version	NM_021576.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	5'-nucleotidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098665.1. On Jun 10, 2007 this sequence version replaced NM_021576.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098665.1, J05214.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGTCCCG CGGCTGCTAC GGCACCCAAG TGGCTGCTTC TCGCGCTAAG CGCTCTACTA 
CCACTGTGGC CCACAGCCAA ATCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC 
AGCCGGCTAG AGCAAACCAG CGATGACTCC ACCAAGTGCC TCAACGCAAG CCTGTGTGTG 
GGCGGCGTGG CCCGGCTCTT CACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTA 
CTGCTTTTGG ATGCTGGCGA TCAGTACCAG GGCACCATCT GGTTCACTGT TTACAAAGGC 
CTGGAAGTGG CACACTTCAT GAACCTCCTG GGCTACGATG CCATGGCACT GGGAAATCAT 
GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAATGT CAAATTTCCA 
ATTCTGAGTG CAAATATTAA GGCCCGGGGG CCACTAGCAC CTCAGATATC CGGACTTTAT 
TTGCCATATA AAGTTCTCTC TGTCGGTGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA 
AAGGAAACCC CCTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTCGAAGA TGAAGTCACT 
GCGTTGCAGC CTGAAGTGGA TAAACTAAAA ACTCTAAACG TGAACAAGAT CATTGCCCTG 
GGACACTCTG GTTTCGAGAT GGATAAACTC ATTGCCCAGA AGGTGAGAGG CGTGGACGTC 
GTGGTGGGAG GACACACCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG 
CCTGCTGGGA AGTACCCATT CATAGTCACC TCTGACGATG GGCGAAAGGT TCCCGTGGTC 
CAGGCCTATG CCTTTGGCAA ATACCTGGGC TATCTGAAGG TTGAGTTTGA TGATAAAGGA 
AATGTTGTCA CTTCCTATGG AAATCCCATT CTTCTGAACA GCACCATTCG TGAAGATGCA 
GCCATCAAAG CAGACATTAA CCAGTGGAGG ATAAAATTAG ATAATTATTC TACACAGGAA 
CTCGGGAGAA CCATCGTCTA CCTGAATGGC TCCGCTCAGG AGTGCCGCTT CAGAGAGTGC 
AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA 
ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAATGGAG GTGGCATCCG GTCCCCCATT 
GATGAGAGGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA 
GGGACATTTG ACCTTGTCCA ATTGAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGT 
GTGCATCGAT ATGGCCAGTC CACAGGGGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG 
TATGATATTT CCCGAAAGCC CTGGGACAGA GTGGTCCAAT TAAAAGTTCT CTGCACCAAG 
TGTCGAGTGC CCATCTATGA GCCTCTCGAA ATGGATAAAG TGTATAAAGT GGTCCTCCCA 
AGCTATCTGG TCAACGGTGG GGATGGATTC CAAATGATAA AAGATGAATT ATTAAAACAT 
GACTCTGGTG ACCAAGATAT CAGTGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAATT 
TACCCAGCAG TTGAAGGACG GATCAAGTTC TCTGCAGCAA GTCATTACCA AGGAAGCTTT 
CCTTTAATAA TTCTTTCCTT TTGGGCAGTG ATCCTTGTTT TGTACCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067587.2
CDS	36..1766
Translation	MRPAAATAPKWLLLALSALLPLWPTAKSWELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLLLDAGDQYQGTIWFTVYKGLEVAHFMNLLGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAPQISGLYLPYKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEVTALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHTNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKVEFDDKGNVVTSYGNPILLNSTIREDAAIKADINQWRIKLDNYSTQELGRTIVYLNGSAQECRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDISRKPWDRVVQLKVLCTKCRVPIYEPLEMDKVYKVVLPSYLVNGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVIYPAVEGRIKFSAASHYQGSFPLIILSFWAVILVLYQ

Target ORF information:

RefSeq Version	NM_021576.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5' nucleotidase, ecto (Nt5e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021576.2

ORF Insert Sequence:

ATGCGTCCCG CGGCTGCTAC GGCACCCAAG TGGCTGCTTC TCGCGCTAAG CGCTCTACTA 
CCACTGTGGC CCACAGCCAA ATCCTGGGAG CTCACGATCC TGCACACAAA CGACGTGCAC 
AGCCGGCTAG AGCAAACCAG CGATGACTCC ACCAAGTGCC TCAACGCAAG CCTGTGTGTG 
GGCGGCGTGG CCCGGCTCTT CACCAAGGTG CAGCAGATCC GCAAGGAAGA ACCCAACGTA 
CTGCTTTTGG ATGCTGGCGA TCAGTACCAG GGCACCATCT GGTTCACTGT TTACAAAGGC 
CTGGAAGTGG CACACTTCAT GAACCTCCTG GGCTACGATG CCATGGCACT GGGAAATCAT 
GAATTTGATA ACGGTGTGGA AGGACTGATT GATCCCCTCC TCAGAAATGT CAAATTTCCA 
ATTCTGAGTG CAAATATTAA GGCCCGGGGG CCACTAGCAC CTCAGATATC CGGACTTTAT 
TTGCCATATA AAGTTCTCTC TGTCGGTGGT GAGGTTGTGG GGATTGTTGG ATATACTTCA 
AAGGAAACCC CCTTCCTCTC AAATCCAGGG ACAAATTTAG TCTTCGAAGA TGAAGTCACT 
GCGTTGCAGC CTGAAGTGGA TAAACTAAAA ACTCTAAACG TGAACAAGAT CATTGCCCTG 
GGACACTCTG GTTTCGAGAT GGATAAACTC ATTGCCCAGA AGGTGAGAGG CGTGGACGTC 
GTGGTGGGAG GACACACCAA CACCTTTCTC TACACAGGAA ATCCACCTTC CAAAGAAGTG 
CCTGCTGGGA AGTACCCATT CATAGTCACC TCTGACGATG GGCGAAAGGT TCCCGTGGTC 
CAGGCCTATG CCTTTGGCAA ATACCTGGGC TATCTGAAGG TTGAGTTTGA TGATAAAGGA 
AATGTTGTCA CTTCCTATGG AAATCCCATT CTTCTGAACA GCACCATTCG TGAAGATGCA 
GCCATCAAAG CAGACATTAA CCAGTGGAGG ATAAAATTAG ATAATTATTC TACACAGGAA 
CTCGGGAGAA CCATCGTCTA CCTGAATGGC TCCGCTCAGG AGTGCCGCTT CAGAGAGTGC 
AACATGGGAA ACCTGATCTG TGATGCCATG ATTAACAACA ACCTCAGACA CCCAGATGAA 
ATGTTTTGGA ACCACGTGTC CATGTGCATT GTAAATGGAG GTGGCATCCG GTCCCCCATT 
GATGAGAGGA ACAATGGTAC CATCACCTGG GAGAACCTGG CTGCTGTGCT GCCCTTTGGA 
GGGACATTTG ACCTTGTCCA ATTGAAAGGG TCCACCCTGA AGAAGGCTTT TGAGCACAGT 
GTGCATCGAT ATGGCCAGTC CACAGGGGAG TTCCTGCAAG TGGGTGGAAT CCATGTGGTG 
TATGATATTT CCCGAAAGCC CTGGGACAGA GTGGTCCAAT TAAAAGTTCT CTGCACCAAG 
TGTCGAGTGC CCATCTATGA GCCTCTCGAA ATGGATAAAG TGTATAAAGT GGTCCTCCCA 
AGCTATCTGG TCAACGGTGG GGATGGATTC CAAATGATAA AAGATGAATT ATTAAAACAT 
GACTCTGGTG ACCAAGATAT CAGTGTGGTT TCTGAATACA TCTCAAAAAT GAAAGTAATT 
TACCCAGCAG TTGAAGGACG GATCAAGTTC TCTGCAGCAA GTCATTACCA AGGAAGCTTT 
CCTTTAATAA TTCTTTCCTT TTGGGCAGTG ATCCTTGTTT TGTACCAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73
Scientific reports7(1)4838(2017 Jul)
Suto EG,Mabuchi Y,Suzuki N,Suzuki K,Ogata Y,Taguchi M,Muneta T,Sekiya I,Akazawa C

CD73-derived adenosine and tenascin-C control cytokine production by epicardium-derived cells formed after myocardial infarction.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(7)3040-3053(2017 Jul)
Hesse J,Leberling S,Boden E,Friebe D,Schmidt T,Ding Z,Dieterich P,Deussen A,Roderigo C,Rose CR,Floss DM,Scheller J,Schrader J

Ecto-5'-nucleotidase (CD73) regulates peripheral chemoreceptor activity and cardiorespiratory responses to hypoxia.
The Journal of physiology(2017 May)
Holmes AP,Ray CJ,Pearson SA,Coney AM,Kumar P

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie84559-568(2016 Dec)
Maciel RM,Carvalho FB,Olabiyi AA,Schmatz R,Gutierres JM,Stefanello N,Zanini D,Rosa MM,Andrade CM,Rubin MA,Schetinger MR,Morsch VM,Danesi CC,Lopes STA

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Nt5e cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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