Ndufaf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndufaf3
Entrez Gene ID	56769
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 3
Synonyms	RGD708545
General protein information	Preferred Names NADH:ubiquinone oxidoreductase complex assembly factor 3 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 NADH dehydrogenase (ubiquinone) complex I, assembly factor 3 nuclear protein E3-3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	This gene encodes a protein that is required for assembly of mitochondrial NADH: ubiquinone oxidoreductase complex I, a multiprotein enzyme complex that is part of the oxidative phosphorylation pathway. In humans, mutations in this gene are associated with fatal neonatal mitochondrial disease and lactic acidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001033971.2	NP_001029143.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
NM_020080.3	NP_064465.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDUFAF3	NP_951032.1
Rattus norvegicus (Norway rat)	Ndufaf3	NP_064465.1
Drosophila melanogaster (fruit fly)	CG5569	NP_611840.1
Pan troglodytes (chimpanzee)	NDUFAF3	XP_001162332.1
Canis lupus familiaris (dog)	NDUFAF3	XP_533836.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003819	XP_310380.2
Caenorhabditis elegans (roundworm)	nuaf-3	NP_001023329.1
	NDUFAF3	XP_001111675.1
Bos taurus (cattle)	NDUFAF3	NP_001039570.1
Mus musculus (house mouse)	Ndufaf3	NP_075736.1
Danio rerio (zebrafish)	ndufaf3	NP_001074084.1
Xenopus tropicalis (tropical clawed frog)	ndufaf3	NP_001005023.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
Saada A, Vogel RO, Hoefs SJ, van den Brand MA, Wessels HJ, Willems PH, Venselaar H, Shaag A, Barghuti F, Reish O, Shohat M, Huynen MA, Smeitink JA, van den Heuvel LP, Nijtmans LG
American journal of human genetics84(6)718-27(2009 Jun)

2P1, a novel male mouse cDNA specifically expressed during meiosis.
Hammami-Hamza S, Doussau M, Allemand I, Segretain D, Gasc JM, Finaz C
The International journal of developmental biology47(1)71-6(2003 Feb)

Molecular cloning of a novel alternatively spliced nuclear protein.
Hartmann AM, Stamm S
Biochimica et biophysica acta1353(3)224-30(1997 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ndufaf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndufaf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12391	NM_001033971.2 Latest version!	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa12419	NM_020080.3 Latest version!	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndufaf3 ( NM_001033971.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029143.1 Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12391
Clone ID Related Accession (Same CDS sequence)	NM_001033971.2
Accession Version	NM_001033971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107280.6 and BC079306.1. On Mar 24, 2015 this sequence version replaced NM_001033971.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: BC079306.1, FM076125.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG CTCTAGGATT TCGCTGCCTT TACCGAACGC GACCTGCGAC GCTCGGTCGG 
AGGAGCCCGA GTCGGGGGCA CCGGCTTTCT CCGGCTGACG ATGAGTTATA CCAGCGGACG 
CGTATCTCTT TGCTGCAAAA CGAGTTCCCT CAGGCCGTAT ACATCGACAG CTACAATAGC 
AGAGGTTTCA CTATCAACGG AAACCGCGTG TTTGGACCTT GTGCGCTGCT CCCACAGACC 
GTGGTCCAGT GGAACGTGGG ATCTCATCAG GATATCACTG AAGAGAGCTT CTCGATTTTC 
TGGATGCTGG AACCTAGGAT AGAGATTGTT GTGGTGGGCA CCGGAAACAA GACTGAGCGG 
CTGCACTCTC AAGTCCTGCA GGCCATGAGG CAGCGGGGAA TTGCGGTGGA GATACAGGAC 
ACGCCTAACG CCTGTGCCAC CTTCAACTTC CTGTGCCACG AAGGTCGAGT GACAGGAGCT 
GCCCTCATCC CCCCACCTGG AGAGACTGCA CTTGCCTCTT CTGGTCAAAC CACAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029143.1
CDS	480..1019
Translation	MATALGFRCLYRTRPATLGRRSPSRGHRLSPADDELYQRTRISLLQNEFPQAVYIDSYNSRGFTINGNRVFGPCALLPQTVVQWNVGSHQDITEESFSIFWMLEPRIEIVVVGTGNKTERLHSQVLQAMRQRGIAVEIQDTPNACATFNFLCHEGRVTGAALIPPPGETALASSGQTTE

Target ORF information:

RefSeq Version	NM_001033971.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033971.2

ORF Insert Sequence:

ATGGCCACTG CTCTAGGATT TCGCTGCCTT TACCGAACGC GACCTGCGAC GCTCGGTCGG 
AGGAGCCCGA GTCGGGGGCA CCGGCTTTCT CCGGCTGACG ATGAGTTATA CCAGCGGACG 
CGTATCTCTT TGCTGCAAAA CGAGTTCCCT CAGGCCGTAT ACATCGACAG CTACAATAGC 
AGAGGTTTCA CTATCAACGG AAACCGCGTG TTTGGACCTT GTGCGCTGCT CCCACAGACC 
GTGGTCCAGT GGAACGTGGG ATCTCATCAG GATATCACTG AAGAGAGCTT CTCGATTTTC 
TGGATGCTGG AACCTAGGAT AGAGATTGTT GTGGTGGGCA CCGGAAACAA GACTGAGCGG 
CTGCACTCTC AAGTCCTGCA GGCCATGAGG CAGCGGGGAA TTGCGGTGGA GATACAGGAC 
ACGCCTAACG CCTGTGCCAC CTTCAACTTC CTGTGCCACG AAGGTCGAGT GACAGGAGCT 
GCCCTCATCC CCCCACCTGG AGAGACTGCA CTTGCCTCTT CTGGTCAAAC CACAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndufaf3 ( NM_020080.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_064465.1 Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12419
Clone ID Related Accession (Same CDS sequence)	NM_020080.3
Accession Version	NM_020080.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107280.6, BC079306.1 and CB609164.1. On Mar 24, 2015 this sequence version replaced NM_020080.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: FQ216095.1, U95160.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCACTG CTCTAGGATT TCGCTGCCTT TACCGAACGC GACCTGCGAC GCTCGGTCGG 
TACGTCGATA GGCTGTGGAG GAGCCCGAGT CGGGGGCACC GGCTTTCTCC GGCTGACGAT 
GAGTTATACC AGCGGACGCG TATCTCTTTG CTGCAAAACG AGTTCCCTCA GGCCGTATAC 
ATCGACAGCT ACAATAGCAG AGGTTTCACT ATCAACGGAA ACCGCGTGTT TGGACCTTGT 
GCGCTGCTCC CACAGACCGT GGTCCAGTGG AACGTGGGAT CTCATCAGGA TATCACTGAA 
GAGAGCTTCT CGATTTTCTG GATGCTGGAA CCTAGGATAG AGATTGTTGT GGTGGGCACC 
GGAAACAAGA CTGAGCGGCT GCACTCTCAA GTCCTGCAGG CCATGAGGCA GCGGGGAATT 
GCGGTGGAGA TACAGGACAC GCCTAACGCC TGTGCCACCT TCAACTTCCT GTGCCACGAA 
GGTCGAGTGA CAGGAGCTGC CCTCATCCCC CCACCTGGAG AGACTGCACT TGCCTCTTCT 
GGTCAAACCA CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064465.1
CDS	480..1037
Translation	MATALGFRCLYRTRPATLGRYVDRLWRSPSRGHRLSPADDELYQRTRISLLQNEFPQAVYIDSYNSRGFTINGNRVFGPCALLPQTVVQWNVGSHQDITEESFSIFWMLEPRIEIVVVGTGNKTERLHSQVLQAMRQRGIAVEIQDTPNACATFNFLCHEGRVTGAALIPPPGETALASSGQTTE

Target ORF information:

RefSeq Version	NM_020080.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NADH:ubiquinone oxidoreductase complex assembly factor 3 (Ndufaf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020080.3

ORF Insert Sequence:

ATGGCCACTG CTCTAGGATT TCGCTGCCTT TACCGAACGC GACCTGCGAC GCTCGGTCGG 
TACGTCGATA GGCTGTGGAG GAGCCCGAGT CGGGGGCACC GGCTTTCTCC GGCTGACGAT 
GAGTTATACC AGCGGACGCG TATCTCTTTG CTGCAAAACG AGTTCCCTCA GGCCGTATAC 
ATCGACAGCT ACAATAGCAG AGGTTTCACT ATCAACGGAA ACCGCGTGTT TGGACCTTGT 
GCGCTGCTCC CACAGACCGT GGTCCAGTGG AACGTGGGAT CTCATCAGGA TATCACTGAA 
GAGAGCTTCT CGATTTTCTG GATGCTGGAA CCTAGGATAG AGATTGTTGT GGTGGGCACC 
GGAAACAAGA CTGAGCGGCT GCACTCTCAA GTCCTGCAGG CCATGAGGCA GCGGGGAATT 
GCGGTGGAGA TACAGGACAC GCCTAACGCC TGTGCCACCT TCAACTTCCT GTGCCACGAA 
GGTCGAGTGA CAGGAGCTGC CCTCATCCCC CCACCTGGAG AGACTGCACT TGCCTCTTCT 
GGTCAAACCA CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.
American journal of human genetics84(6)718-27(2009 Jun)
Saada A,Vogel RO,Hoefs SJ,van den Brand MA,Wessels HJ,Willems PH,Venselaar H,Shaag A,Barghuti F,Reish O,Shohat M,Huynen MA,Smeitink JA,van den Heuvel LP,Nijtmans LG

2P1, a novel male mouse cDNA specifically expressed during meiosis.
The International journal of developmental biology47(1)71-6(2003 Feb)
Hammami-Hamza S,Doussau M,Allemand I,Segretain D,Gasc JM,Finaz C

Molecular cloning of a novel alternatively spliced nuclear protein.
Biochimica et biophysica acta1353(3)224-30(1997 Sep)
Hartmann AM,Stamm S

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Ndufaf3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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