gRNAs
The following Pclo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pclo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa12686 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001110797.1 | |
| Accession Version | NM_001110797.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 14643bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-20 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | protein piccolo isoform 2 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF138789.2. Transcript Variant: This variant (2) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF138789.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001104267.1 |
| CDS | 164..14806 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001110797.1 |
1 | ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa12687 | |
| Clone ID Related Accession (Same CDS sequence) | NM_020098.1 | |
| Accession Version | NM_020098.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 15258bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-26 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | protein piccolo isoform 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF227534.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF227534.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_064483.1 |
| CDS | 164..15421 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_020098.1 |
1 | ATGGGCAACG AGGCGAGCTT GGAAGGGGAA GGGCTCCCCG AAGGGCTGGC GGCGGCCGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The presynaptic scaffolding protein Piccolo organizes the readily releasable pool at the calyx of Held. |
Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. |
Synaptic activity controls localization and function of CtBP1 via binding to Bassoon and Piccolo. |
Piccolo Directs Activity Dependent F-Actin Assembly from Presynaptic Active Zones via Daam1. |
Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone. |
