Espn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Espn
Entrez Gene ID	56227
Full Name	espin
Synonyms	Je
General protein information	Preferred Names espin Names espin Jerker, deafness locus actin cytoskeletal regulatory protein ectoplasmic specialization protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	is a fusion protein which binds actin filaments to each other and to the membranes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239484.3	XP_006239546.1	espin isoform X6
XM_008764377.1	XP_008762599.1	espin isoform X1
XM_017593602.1	XP_017449091.1	espin isoform X2
XM_008764378.1	XP_008762600.1	espin isoform X3
NM_019622.2	NP_062568.2	espin
XM_006239479.2	XP_006239541.1	espin isoform X4
XM_008764380.1	XP_008762602.1	espin isoform X5

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ESPN	XP_417532.4
Xenopus tropicalis (tropical clawed frog)	espn	XP_004916250.1
Homo sapiens (human)	ESPN	NP_113663.2
Mus musculus (house mouse)	Espn	NP_997570.1
Pan troglodytes (chimpanzee)	ESPN	XP_003307841.2
Canis lupus familiaris (dog)	ESPN	XP_005620513.1
Rattus norvegicus (Norway rat)	Espn	NP_062568.1
Danio rerio (zebrafish)	espn	NP_001116754.1

Gene Ontology
Bibliography

Related articles in PubMed

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
Lelli A, Michel V, Boutet de Monvel J, Cortese M, Bosch-Grau M, Aghaie A, Perfettini I, Dupont T, Avan P, El-Amraoui A, Petit C
The Journal of cell biology212(2)231-44(2016 Jan)

Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.
Merritt RC, Manor U, Salles FT, Grati M, Dose AC, Unrath WC, Quintero OA, Yengo CM, Kachar B
Current biology : CB22(4)320-5(2012 Feb)

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Revenu C, Ubelmann F, Hurbain I, El-Marjou F, Dingli F, Loew D, Delacour D, Gilet J, Brot-Laroche E, Rivero F, Louvard D, Robine S
Molecular biology of the cell23(2)324-36(2012 Jan)

Review series: The cell biology of hearing.
Schwander M, Kachar B, M?ller U
The Journal of cell biology190(1)9-20(2010 Jul)

Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing.
Kitajiri S, Sakamoto T, Belyantseva IA, Goodyear RJ, Stepanyan R, Fujiwara I, Bird JE, Riazuddin S, Riazuddin S, Ahmed ZM, Hinshaw JE, Sellers J, Bartles JR, Hammer JA 3rd, Richardson GP, Griffith AJ, Frolenkov GI, Friedman TB
Cell141(5)786-98(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study provides the basis for understanding the role of myosin IIIa and espin 1 in regulating stereocilia length.
Title: Myosin IIIa boosts elongation of stereocilia by transporting espin 1 to the plus ends of actin filaments.

Localization of espins to a large proportion of rat type I spiral ganglion neurons (SGNs) and their projections to the cochlear nucleus.
Title: Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal.

Espin is expressed in the epididymis and is regulated by androgens.
Title: Microvillar size and espin expression in principal cells of the adult rat epididymis are regulated by androgens.

Following hypophysectomy, the actin cytoskeleton of the Sertoli cell shifts to a predominantly depolymerized state, perhaps in part because of decreases in profilin II and espin gene products.
Title: An ex vivo analysis of Sertoli cell actin dynamics following gonadotropic hormone withdrawal.

The following Espn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Espn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa28945	XM_006239484.3 Latest version!	Rattus norvegicus espin (Espn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa43445	NM_019622.2 Latest version!	Rattus norvegicus espin (Espn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa28940	XM_006239479.2 Latest version!	Rattus norvegicus espin (Espn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa43441	XM_008764377.1 Latest version!	Rattus norvegicus espin (Espn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa43442	XM_008764378.1 Latest version!	Rattus norvegicus espin (Espn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa43444	XM_008764380.1 Latest version!	Rattus norvegicus espin (Espn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa61367	XM_017593602.1 Latest version!	Rattus norvegicus espin (Espn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Espn ( XM_006239484.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239546.1 Rattus norvegicus espin (Espn), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa28945
Clone ID Related Accession (Same CDS sequence)	XM_006239484.3
Accession Version	XM_006239484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCCC AGGGGCCTCT ACGTGGGGGC CGAATGCCCA GCACCAAATC TTTCAACATG 
ATGTCCCCAA CGGGTGACAA CTCGGAGTTG CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG 
AAACCAACAC CGCAGAGCAA GGGGCTGACA ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC 
TCCCAGGTAG GCACTGGACG GGTGCCCCGC CCGGGCTCCC AGTGCCTGCC CAGTGCTCAG 
CCCTACCGCT TCTCCCGGCA GCCTGAGTCA CCACAGCCTG CGGTGTCACC TGGGCCATCT 
CGGGCCCGGA GCCCCACCCC ACCAGCCTCT GGGCCTCAGC CACTGCTCAA TGGCAGCATA 
GTACCGGCAC CACCTGCCAC CCTAGCACCA GGAGTGCATC TGGATGTGGA GGCCCTCATC 
CCCACACTTG ATGAGCAGGG CCGGCCCATC CCGGAGTGGA AGCGCCAGGT GATGGTCCGG 
AAGCTGCAGC AGAAAATGCA GGAGGAAGAG GAGCAGCGGA GGAAGGAAGA AGAGGAGGAG 
GCCCGGCTGG CCAGCCTGCC CGCCTGGAGA CGAGACATTC TTAGGAAGAA GCTGGAGGAG 
GAGAGGGAGC AGAAGCGAAA AGAGGAGGAG CGGCAGAAGC TGGAGGAAAT CCAGAGGGCG 
AAAGAACAGT CGGAGAAGCT GCGGACACTA GGATACGACG AGGCCAAGCT CGCGCCCTGG 
CAGCGACAGG TCATCTTGAA GAAGGGGGAG ATCCCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239546.1
CDS	685..1446
Translation	MNSQGPLRGGRMPSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQVGTGRVPRPGSQCLPSAQPYRFSRQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_006239484.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239484.3

ORF Insert Sequence:

ATGAACTCCC AGGGGCCTCT ACGTGGGGGC CGAATGCCCA GCACCAAATC TTTCAACATG 
ATGTCCCCAA CGGGTGACAA CTCGGAGTTG CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG 
AAACCAACAC CGCAGAGCAA GGGGCTGACA ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC 
TCCCAGGTAG GCACTGGACG GGTGCCCCGC CCGGGCTCCC AGTGCCTGCC CAGTGCTCAG 
CCCTACCGCT TCTCCCGGCA GCCTGAGTCA CCACAGCCTG CGGTGTCACC TGGGCCATCT 
CGGGCCCGGA GCCCCACCCC ACCAGCCTCT GGGCCTCAGC CACTGCTCAA TGGCAGCATA 
GTACCGGCAC CACCTGCCAC CCTAGCACCA GGAGTGCATC TGGATGTGGA GGCCCTCATC 
CCCACACTTG ATGAGCAGGG CCGGCCCATC CCGGAGTGGA AGCGCCAGGT GATGGTCCGG 
AAGCTGCAGC AGAAAATGCA GGAGGAAGAG GAGCAGCGGA GGAAGGAAGA AGAGGAGGAG 
GCCCGGCTGG CCAGCCTGCC CGCCTGGAGA CGAGACATTC TTAGGAAGAA GCTGGAGGAG 
GAGAGGGAGC AGAAGCGAAA AGAGGAGGAG CGGCAGAAGC TGGAGGAAAT CCAGAGGGCG 
AAAGAACAGT CGGAGAAGCT GCGGACACTA GGATACGACG AGGCCAAGCT CGCGCCCTGG 
CAGCGACAGG TCATCTTGAA GAAGGGGGAG ATCCCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( NM_019622.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062568.2 Rattus norvegicus espin (Espn), mRNA.

Price & Availability↑

CloneID	ORa43445
Clone ID Related Accession (Same CDS sequence)	NM_019622.2
Accession Version	NM_019622.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	espin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07050551.1 and AABR07050550.1. On Feb 5, 2015 this sequence version replaced NM_019622.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U46007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGCG 
GCGCTGCTCC CCGGGAACCA CGTGCACAAC GGCTGCTCCG CGGACTCCAA AGCGTCCAGG 
GAGCTGCCGC CGCCACCGCC GCCGCCGCCC CTGCCCGAGG CCCTGAGTTC GCCGCCACCC 
GCCCCACCTC TGCCCATCGA GGGCGCGGGC GCAGCCTGCG GACAGCGGCG TTCCTCATCT 
TCTACTGGCA GCACCAAATC TTTCAACATG ATGTCCCCAA CGGGTGACAA CTCGGAGTTG 
CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG AAACCAACAC CGCAGAGCAA GGGGCTGACA 
ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC TCCCAGCCTG AGTCACCACA GCCTGCGGTG 
TCACCTGGGC CATCTCGGGC CCGGAGCCCC ACCCCACCAG CCTCTGGGCC TCAGCCACTG 
CTCAATGGCA GCATAGTACC GGCACCACCT GCCACCCTAG CACCAGGAGT GCATCTGGAT 
GTGGAGGCCC TCATCCCCAC ACTTGATGAG CAGGGCCGGC CCATCCCGGA GTGGAAGCGC 
CAGGTGATGG TCCGGAAGCT GCAGCAGAAA ATGCAGGAGG AAGAGGAGCA GCGGAGGAAG 
GAAGAAGAGG AGGAGGCCCG GCTGGCCAGC CTGCCCGCCT GGAGACGAGA CATTCTTAGG 
AAGAAGCTGG AGGAGGAGAG GGAGCAGAAG CGAAAAGAGG AGGAGCGGCA GAAGCTGGAG 
GAAATCCAGA GGGCGAAAGA ACAGTCGGAG AAGCTGCGGA CACTAGGATA CGACGAGGCC 
AAGCTCGCGC CCTGGCAGCG ACAGGTCATC TTGAAGAAGG GGGAGATCCC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062568.2
CDS	16..2532
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	NM_019622.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019622.2

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGCG 
GCGCTGCTCC CCGGGAACCA CGTGCACAAC GGCTGCTCCG CGGACTCCAA AGCGTCCAGG 
GAGCTGCCGC CGCCACCGCC GCCGCCGCCC CTGCCCGAGG CCCTGAGTTC GCCGCCACCC 
GCCCCACCTC TGCCCATCGA GGGCGCGGGC GCAGCCTGCG GACAGCGGCG TTCCTCATCT 
TCTACTGGCA GCACCAAATC TTTCAACATG ATGTCCCCAA CGGGTGACAA CTCGGAGTTG 
CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG AAACCAACAC CGCAGAGCAA GGGGCTGACA 
ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC TCCCAGCCTG AGTCACCACA GCCTGCGGTG 
TCACCTGGGC CATCTCGGGC CCGGAGCCCC ACCCCACCAG CCTCTGGGCC TCAGCCACTG 
CTCAATGGCA GCATAGTACC GGCACCACCT GCCACCCTAG CACCAGGAGT GCATCTGGAT 
GTGGAGGCCC TCATCCCCAC ACTTGATGAG CAGGGCCGGC CCATCCCGGA GTGGAAGCGC 
CAGGTGATGG TCCGGAAGCT GCAGCAGAAA ATGCAGGAGG AAGAGGAGCA GCGGAGGAAG 
GAAGAAGAGG AGGAGGCCCG GCTGGCCAGC CTGCCCGCCT GGAGACGAGA CATTCTTAGG 
AAGAAGCTGG AGGAGGAGAG GGAGCAGAAG CGAAAAGAGG AGGAGCGGCA GAAGCTGGAG 
GAAATCCAGA GGGCGAAAGA ACAGTCGGAG AAGCTGCGGA CACTAGGATA CGACGAGGCC 
AAGCTCGCGC CCTGGCAGCG ACAGGTCATC TTGAAGAAGG GGGAGATCCC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_006239479.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239541.1 Rattus norvegicus espin (Espn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa28940
Clone ID Related Accession (Same CDS sequence)	XM_006239479.2
Accession Version	XM_006239479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006239479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGCG 
GCGCTGCTCC CCGGGAACCA CGTGCACAAC GGCTGCTCCG CGGACTCCAA AGCGTCCAGG 
GAGCTGCCGC CGCCACCGCC GCCGCCGCCC CTGCCCGAGG CCCTGAGTTC GCCGCCACCC 
GCCCCACCTC TGCCCATCGA GGGCGCGGGC GCAGCCTGCG GACAGCGGCG TTCCTCATCT 
TCTACTGGCA GCACCAAATC TTTCAACATG ATGTCCCCAA CGGGTGACAA CTCGGAGTTG 
CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG AAACCAACAC CGCAGAGCAA GGGGCTGACA 
ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC TCCCAGGTAG GCACTGGACG GGTGCCCCGC 
CCGGGCTCCC AGTGCCTGCC CAGTGCTCAG CCCTACCGCT TCTCCCGGCA GCCTGAGTCA 
CCACAGCCTG CGGTGTCACC TGGGCCATCT CGGGCCCGGA GCCCCACCCC ACCAGCCTCT 
GGGCCTCAGC CACTGCTCAA TGGCAGCATA GTACCGGCAC CACCTGCCAC CCTAGCACCA 
GGAGTGCATC TGGATGTGGA GGCCCTCATC CCCACACTTG ATGAGCAGGG CCGGCCCATC 
CCGGAGTGGA AGCGCCAGGT GATGGTCCGG AAGCTGCAGC AGAAAATGCA GGAGGAAGAG 
GAGCAGCGGA GGAAGGAAGA AGAGGAGGAG GCCCGGCTGG CCAGCCTGCC CGCCTGGAGA 
CGAGACATTC TTAGGAAGAA GCTGGAGGAG GAGAGGGAGC AGAAGCGAAA AGAGGAGGAG 
CGGCAGAAGC TGGAGGAAAT CCAGAGGGCG AAAGAACAGT CGGAGAAGCT GCGGACACTA 
GGATACGACG AGGCCAAGCT CGCGCCCTGG CAGCGACAGG TCATCTTGAA GAAGGGGGAG 
ATCCCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239541.1
CDS	191..2782
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQVGTGRVPRPGSQCLPSAQPYRFSRQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_006239479.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239479.2

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGCG 
GCGCTGCTCC CCGGGAACCA CGTGCACAAC GGCTGCTCCG CGGACTCCAA AGCGTCCAGG 
GAGCTGCCGC CGCCACCGCC GCCGCCGCCC CTGCCCGAGG CCCTGAGTTC GCCGCCACCC 
GCCCCACCTC TGCCCATCGA GGGCGCGGGC GCAGCCTGCG GACAGCGGCG TTCCTCATCT 
TCTACTGGCA GCACCAAATC TTTCAACATG ATGTCCCCAA CGGGTGACAA CTCGGAGTTG 
CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG AAACCAACAC CGCAGAGCAA GGGGCTGACA 
ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC TCCCAGGTAG GCACTGGACG GGTGCCCCGC 
CCGGGCTCCC AGTGCCTGCC CAGTGCTCAG CCCTACCGCT TCTCCCGGCA GCCTGAGTCA 
CCACAGCCTG CGGTGTCACC TGGGCCATCT CGGGCCCGGA GCCCCACCCC ACCAGCCTCT 
GGGCCTCAGC CACTGCTCAA TGGCAGCATA GTACCGGCAC CACCTGCCAC CCTAGCACCA 
GGAGTGCATC TGGATGTGGA GGCCCTCATC CCCACACTTG ATGAGCAGGG CCGGCCCATC 
CCGGAGTGGA AGCGCCAGGT GATGGTCCGG AAGCTGCAGC AGAAAATGCA GGAGGAAGAG 
GAGCAGCGGA GGAAGGAAGA AGAGGAGGAG GCCCGGCTGG CCAGCCTGCC CGCCTGGAGA 
CGAGACATTC TTAGGAAGAA GCTGGAGGAG GAGAGGGAGC AGAAGCGAAA AGAGGAGGAG 
CGGCAGAAGC TGGAGGAAAT CCAGAGGGCG AAAGAACAGT CGGAGAAGCT GCGGACACTA 
GGATACGACG AGGCCAAGCT CGCGCCCTGG CAGCGACAGG TCATCTTGAA GAAGGGGGAG 
ATCCCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_008764377.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762599.1 Rattus norvegicus espin (Espn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43441
Clone ID Related Accession (Same CDS sequence)	XM_008764377.1
Accession Version	XM_008764377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGTGC GTGTCCACCA GCCCGCACCT GCGGGCTGCA CAGGATCGAA CCCTGTCTCA 
CACTCCTCAC TCTCTGGCCC CTCTGCTCCC CCGCAGGCGG CGCTGCTCCC CGGGAACCAC 
GTGCACAACG GCTGCTCCGC GGACTCCAAA GCGTCCAGGG AGCTGCCGCC GCCACCGCCG 
CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG CCGCCACCCG CCCCACCTCT GCCCATCGAG 
GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT TCCTCATCTT CTACTGGCAG CACCAAATCT 
TTCAACATGA TGTCCCCAAC GGGTGACAAC TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC 
AAGAGTCTGA AACCAACACC GCAGAGCAAG GGGCTGACAA CAGTGTTCTC AGGCAGCGGG 
CAGCCAGCCT CCCAGCCTGA GTCACCACAG CCTGCGGTGT CACCTGGGCC ATCTCGGGCC 
CGGAGCCCCA CCCCACCAGC CTCTGGGCCT CAGCCACTGC TCAATGGCAG CATAGTACCG 
GCACCACCTG CCACCCTAGC ACCAGGAGTG CATCTGGATG TGGAGGCCCT CATCCCCACA 
CTTGATGAGC AGGGCCGGCC CATCCCGGAG TGGAAGCGCC AGGTGATGGT CCGGAAGCTG 
CAGCAGAAAA TGCAGGAGGA AGAGGAGCAG CGGAGGAAGG AAGAAGAGGA GGAGGCCCGG 
CTGGCCAGCC TGCCCGCCTG GAGACGAGAC ATTCTTAGGA AGAAGCTGGA GGAGGAGAGG 
GAGCAGAAGC GAAAAGAGGA GGAGCGGCAG AAGCTGGAGG AAATCCAGAG GGCGAAAGAA 
CAGTCGGAGA AGCTGCGGAC ACTAGGATAC GACGAGGCCA AGCTCGCGCC CTGGCAGCGA 
CAGGTCATCT TGAAGAAGGG GGAGATCCCT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762599.1
CDS	191..2806
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGVPGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEVRVHQPAPAGCTGSNPVSHSSLSGPSAPPQAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_008764377.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764377.1

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGTGC GTGTCCACCA GCCCGCACCT GCGGGCTGCA CAGGATCGAA CCCTGTCTCA 
CACTCCTCAC TCTCTGGCCC CTCTGCTCCC CCGCAGGCGG CGCTGCTCCC CGGGAACCAC 
GTGCACAACG GCTGCTCCGC GGACTCCAAA GCGTCCAGGG AGCTGCCGCC GCCACCGCCG 
CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG CCGCCACCCG CCCCACCTCT GCCCATCGAG 
GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT TCCTCATCTT CTACTGGCAG CACCAAATCT 
TTCAACATGA TGTCCCCAAC GGGTGACAAC TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC 
AAGAGTCTGA AACCAACACC GCAGAGCAAG GGGCTGACAA CAGTGTTCTC AGGCAGCGGG 
CAGCCAGCCT CCCAGCCTGA GTCACCACAG CCTGCGGTGT CACCTGGGCC ATCTCGGGCC 
CGGAGCCCCA CCCCACCAGC CTCTGGGCCT CAGCCACTGC TCAATGGCAG CATAGTACCG 
GCACCACCTG CCACCCTAGC ACCAGGAGTG CATCTGGATG TGGAGGCCCT CATCCCCACA 
CTTGATGAGC AGGGCCGGCC CATCCCGGAG TGGAAGCGCC AGGTGATGGT CCGGAAGCTG 
CAGCAGAAAA TGCAGGAGGA AGAGGAGCAG CGGAGGAAGG AAGAAGAGGA GGAGGCCCGG 
CTGGCCAGCC TGCCCGCCTG GAGACGAGAC ATTCTTAGGA AGAAGCTGGA GGAGGAGAGG 
GAGCAGAAGC GAAAAGAGGA GGAGCGGCAG AAGCTGGAGG AAATCCAGAG GGCGAAAGAA 
CAGTCGGAGA AGCTGCGGAC ACTAGGATAC GACGAGGCCA AGCTCGCGCC CTGGCAGCGA 
CAGGTCATCT TGAAGAAGGG GGAGATCCCT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_008764378.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762600.1 Rattus norvegicus espin (Espn), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43442
Clone ID Related Accession (Same CDS sequence)	XM_008764378.1
Accession Version	XM_008764378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGCGG CGCTGCTCCC CGGGAACCAC GTGCACAACG GCTGCTCCGC GGACTCCAAA 
GCGTCCAGGG AGCTGCCGCC GCCACCGCCG CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG 
CCGCCACCCG CCCCACCTCT GCCCATCGAG GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT 
TCCTCATCTT CTACTGGCAG CACCAAATCT TTCAACATGA TGTCCCCAAC GGGTGACAAC 
TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC AAGAGTCTGA AACCAACACC GCAGAGCAAG 
GGGCTGACAA CAGTGTTCTC AGGCAGCGGG CAGCCAGCCT CCCAGGTAGG CACTGGACGG 
GTGCCCCGCC CGGGCTCCCA GTGCCTGCCC AGTGCTCAGC CCTACCGCTT CTCCCGGCAG 
CCTGAGTCAC CACAGCCTGC GGTGTCACCT GGGCCATCTC GGGCCCGGAG CCCCACCCCA 
CCAGCCTCTG GGCCTCAGCC ACTGCTCAAT GGCAGCATAG TACCGGCACC ACCTGCCACC 
CTAGCACCAG GAGTGCATCT GGATGTGGAG GCCCTCATCC CCACACTTGA TGAGCAGGGC 
CGGCCCATCC CGGAGTGGAA GCGCCAGGTG ATGGTCCGGA AGCTGCAGCA GAAAATGCAG 
GAGGAAGAGG AGCAGCGGAG GAAGGAAGAA GAGGAGGAGG CCCGGCTGGC CAGCCTGCCC 
GCCTGGAGAC GAGACATTCT TAGGAAGAAG CTGGAGGAGG AGAGGGAGCA GAAGCGAAAA 
GAGGAGGAGC GGCAGAAGCT GGAGGAAATC CAGAGGGCGA AAGAACAGTC GGAGAAGCTG 
CGGACACTAG GATACGACGA GGCCAAGCTC GCGCCCTGGC AGCGACAGGT CATCTTGAAG 
AAGGGGGAGA TCCCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762600.1
CDS	191..2791
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGVPGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQVGTGRVPRPGSQCLPSAQPYRFSRQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_008764378.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764378.1

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGCGG CGCTGCTCCC CGGGAACCAC GTGCACAACG GCTGCTCCGC GGACTCCAAA 
GCGTCCAGGG AGCTGCCGCC GCCACCGCCG CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG 
CCGCCACCCG CCCCACCTCT GCCCATCGAG GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT 
TCCTCATCTT CTACTGGCAG CACCAAATCT TTCAACATGA TGTCCCCAAC GGGTGACAAC 
TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC AAGAGTCTGA AACCAACACC GCAGAGCAAG 
GGGCTGACAA CAGTGTTCTC AGGCAGCGGG CAGCCAGCCT CCCAGGTAGG CACTGGACGG 
GTGCCCCGCC CGGGCTCCCA GTGCCTGCCC AGTGCTCAGC CCTACCGCTT CTCCCGGCAG 
CCTGAGTCAC CACAGCCTGC GGTGTCACCT GGGCCATCTC GGGCCCGGAG CCCCACCCCA 
CCAGCCTCTG GGCCTCAGCC ACTGCTCAAT GGCAGCATAG TACCGGCACC ACCTGCCACC 
CTAGCACCAG GAGTGCATCT GGATGTGGAG GCCCTCATCC CCACACTTGA TGAGCAGGGC 
CGGCCCATCC CGGAGTGGAA GCGCCAGGTG ATGGTCCGGA AGCTGCAGCA GAAAATGCAG 
GAGGAAGAGG AGCAGCGGAG GAAGGAAGAA GAGGAGGAGG CCCGGCTGGC CAGCCTGCCC 
GCCTGGAGAC GAGACATTCT TAGGAAGAAG CTGGAGGAGG AGAGGGAGCA GAAGCGAAAA 
GAGGAGGAGC GGCAGAAGCT GGAGGAAATC CAGAGGGCGA AAGAACAGTC GGAGAAGCTG 
CGGACACTAG GATACGACGA GGCCAAGCTC GCGCCCTGGC AGCGACAGGT CATCTTGAAG 
AAGGGGGAGA TCCCTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_008764380.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762602.1 Rattus norvegicus espin (Espn), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa43444
Clone ID Related Accession (Same CDS sequence)	XM_008764380.1
Accession Version	XM_008764380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGCGG CGCTGCTCCC CGGGAACCAC GTGCACAACG GCTGCTCCGC GGACTCCAAA 
GCGTCCAGGG AGCTGCCGCC GCCACCGCCG CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG 
CCGCCACCCG CCCCACCTCT GCCCATCGAG GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT 
TCCTCATCTT CTACTGGCAG CACCAAATCT TTCAACATGA TGTCCCCAAC GGGTGACAAC 
TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC AAGAGTCTGA AACCAACACC GCAGAGCAAG 
GGGCTGACAA CAGTGTTCTC AGGCAGCGGG CAGCCAGCCT CCCAGCCTGA GTCACCACAG 
CCTGCGGTGT CACCTGGGCC ATCTCGGGCC CGGAGCCCCA CCCCACCAGC CTCTGGGCCT 
CAGCCACTGC TCAATGGCAG CATAGTACCG GCACCACCTG CCACCCTAGC ACCAGGAGTG 
CATCTGGATG TGGAGGCCCT CATCCCCACA CTTGATGAGC AGGGCCGGCC CATCCCGGAG 
TGGAAGCGCC AGGTGATGGT CCGGAAGCTG CAGCAGAAAA TGCAGGAGGA AGAGGAGCAG 
CGGAGGAAGG AAGAAGAGGA GGAGGCCCGG CTGGCCAGCC TGCCCGCCTG GAGACGAGAC 
ATTCTTAGGA AGAAGCTGGA GGAGGAGAGG GAGCAGAAGC GAAAAGAGGA GGAGCGGCAG 
AAGCTGGAGG AAATCCAGAG GGCGAAAGAA CAGTCGGAGA AGCTGCGGAC ACTAGGATAC 
GACGAGGCCA AGCTCGCGCC CTGGCAGCGA CAGGTCATCT TGAAGAAGGG GGAGATCCCT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762602.1
CDS	191..2716
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGVPGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_008764380.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764380.1

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGGGTTCCA 
GGTGCCAGAT CCTCAGACTT ACAGAGCTAC ATGGACATGC TGAACCCAGA GCCTCGGAGC 
AAGCAAGGGA AGCCTTCATC TCTACCACCA CCGCCACCAC CAAGCTTCCC TCCACCACCA 
CCCCCAGGCA CACAGCTGCC CCCACCTCCA CCAGGCTACC CAGCTCCCAA TCCCCCTGTG 
GGGCTGCATT TGGATAACAT TTACATGCAG ACCAAGAACA AACTCCGCCA CGTGGAGGTG 
GACTCCCTCA AGAAGGAGCC GAGCTCCGGC GACGGCTACT CGGGGCTACG CAGGCAGGAT 
TCCGGGCTGC TCAGGCAGGA TTCGGAGCTG CTGCTCAGGC ACAACACCGG ACTGCGCAGG 
CAGGACTCCG ACCGCAAACA GCGTTCATTC AGTAAACAGC CCAGCACGGG GGACTACTAC 
CGCCAGCTGG GCCGCAGCCC GGGGGAGCCG CTGGCCGCAC GCCCGGGCAT GGCCCACAGC 
GAGGAGGCGG CGCTGCTCCC CGGGAACCAC GTGCACAACG GCTGCTCCGC GGACTCCAAA 
GCGTCCAGGG AGCTGCCGCC GCCACCGCCG CCGCCGCCCC TGCCCGAGGC CCTGAGTTCG 
CCGCCACCCG CCCCACCTCT GCCCATCGAG GGCGCGGGCG CAGCCTGCGG ACAGCGGCGT 
TCCTCATCTT CTACTGGCAG CACCAAATCT TTCAACATGA TGTCCCCAAC GGGTGACAAC 
TCGGAGTTGC TGGCTGAAAT AAAAGCAGGC AAGAGTCTGA AACCAACACC GCAGAGCAAG 
GGGCTGACAA CAGTGTTCTC AGGCAGCGGG CAGCCAGCCT CCCAGCCTGA GTCACCACAG 
CCTGCGGTGT CACCTGGGCC ATCTCGGGCC CGGAGCCCCA CCCCACCAGC CTCTGGGCCT 
CAGCCACTGC TCAATGGCAG CATAGTACCG GCACCACCTG CCACCCTAGC ACCAGGAGTG 
CATCTGGATG TGGAGGCCCT CATCCCCACA CTTGATGAGC AGGGCCGGCC CATCCCGGAG 
TGGAAGCGCC AGGTGATGGT CCGGAAGCTG CAGCAGAAAA TGCAGGAGGA AGAGGAGCAG 
CGGAGGAAGG AAGAAGAGGA GGAGGCCCGG CTGGCCAGCC TGCCCGCCTG GAGACGAGAC 
ATTCTTAGGA AGAAGCTGGA GGAGGAGAGG GAGCAGAAGC GAAAAGAGGA GGAGCGGCAG 
AAGCTGGAGG AAATCCAGAG GGCGAAAGAA CAGTCGGAGA AGCTGCGGAC ACTAGGATAC 
GACGAGGCCA AGCTCGCGCC CTGGCAGCGA CAGGTCATCT TGAAGAAGGG GGAGATCCCT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Espn ( XM_017593602.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449091.1 Rattus norvegicus espin (Espn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61367
Clone ID Related Accession (Same CDS sequence)	XM_017593602.1
Accession Version	XM_017593602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	espin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGTG 
CGTGTCCACC AGCCCGCACC TGCGGGCTGC ACAGGATCGA ACCCTGTCTC ACACTCCTCA 
CTCTCTGGCC CCTCTGCTCC CCCGCAGGCG GCGCTGCTCC CCGGGAACCA CGTGCACAAC 
GGCTGCTCCG CGGACTCCAA AGCGTCCAGG GAGCTGCCGC CGCCACCGCC GCCGCCGCCC 
CTGCCCGAGG CCCTGAGTTC GCCGCCACCC GCCCCACCTC TGCCCATCGA GGGCGCGGGC 
GCAGCCTGCG GACAGCGGCG TTCCTCATCT TCTACTGGCA GCACCAAATC TTTCAACATG 
ATGTCCCCAA CGGGTGACAA CTCGGAGTTG CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG 
AAACCAACAC CGCAGAGCAA GGGGCTGACA ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC 
TCCCAGCCTG AGTCACCACA GCCTGCGGTG TCACCTGGGC CATCTCGGGC CCGGAGCCCC 
ACCCCACCAG CCTCTGGGCC TCAGCCACTG CTCAATGGCA GCATAGTACC GGCACCACCT 
GCCACCCTAG CACCAGGAGT GCATCTGGAT GTGGAGGCCC TCATCCCCAC ACTTGATGAG 
CAGGGCCGGC CCATCCCGGA GTGGAAGCGC CAGGTGATGG TCCGGAAGCT GCAGCAGAAA 
ATGCAGGAGG AAGAGGAGCA GCGGAGGAAG GAAGAAGAGG AGGAGGCCCG GCTGGCCAGC 
CTGCCCGCCT GGAGACGAGA CATTCTTAGG AAGAAGCTGG AGGAGGAGAG GGAGCAGAAG 
CGAAAAGAGG AGGAGCGGCA GAAGCTGGAG GAAATCCAGA GGGCGAAAGA ACAGTCGGAG 
AAGCTGCGGA CACTAGGATA CGACGAGGCC AAGCTCGCGC CCTGGCAGCG ACAGGTCATC 
TTGAAGAAGG GGGAGATCCC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449091.1
CDS	191..2797
Translation	MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAARSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDNSGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPLHDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEHRVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKGQRSTRGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGYPAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELLLRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEVRVHQPAPAGCTGSNPVSHSSLSGPSAPPQAALLPGNHVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQPESPQPAVSPGPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKRQVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQKLEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK

Target ORF information:

RefSeq Version	XM_017593602.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus espin (Espn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593602.1

ORF Insert Sequence:

ATGGCCCTGG AACAGGCGAT GCAGGCGGCA CGGCGGGGCG ACCTGGACGT GCTGAGGTCC 
CTGCACGCCG CCGGCCTGCT GGGGCCTTCT CTGCGCGACC CGCTAGACGC CCTGCCGGTG 
CACCATGCGG CCCGCTCAGG CAAGCTGCAC TGTTTGCGCT ACTTGGTGGA GGAGGTTGCC 
CTCCCAGCTG TGTCCCGCGC GCGCAACGGC GCCACACCAG CCCATGATGC CGCTGCCACG 
GGCTACCTCT CTTGCCTGCA GTGGCTGCTC ACACAGGGTG GCTGCAGGGT GCAGGAAAAA 
GATAACTCTG GTGCCACGGT CCTGCACCTG GCTGCCCGCT TTGGCCACCC GGACGTGGTG 
AACTGGCTGC TGTACCAGGG CGGTGCGAAC TCTGCCATCA CCACAGACAC GGGCGCCCTG 
CCTATCCACT ATGCTGCCGC CAAAGGAGAT CTCCCCTCCA TGAAGCTTCT TGTCGGGCAC 
TACCCTGAGG GAGTGAATGC CCAAACCAAC AACGGTGCCA CGCCCCTGTA CCTGGCGTGC 
CAGGAGGGCC ACCTGGAAGT GACGAAGTAC CTTGTGCAGG AGTGCAGTGC AGATCCGCAC 
CTGCGCGCCC AAGACGGCAT GACCCCCCTG CATGCCGCGG CGCAGATGGG CCACAACCCA 
GTCCTGGTGT GGCTGGTGAG CTTTGCGGAC GTGAGCTTTT CGGAGCAGGA CCACGACGGC 
GCCACAGCCA TGCACTTTGC AGCCAGCCGC GGCCACACCA AAGTGCTCAG CTGGCTCCTG 
CTGCACGGCG CAGAGATCTC CCAGGACCTG TGGGGCGGGA CCCCGCTGCA TGATGCTGCT 
GAGAACGGGG AACTGGAGTG CTGCCAGATC CTCGCGGTGA ATGGTGCGGG GCTGGACGTC 
CGCGACCACG ATGGGTATAC GGCTGCGGAC CTGGCTGATT TCAATGGCCA CACCCACTGC 
TCCCGCTACC TACGTACGGT GCAAACCCTG AGCTTGGAAC ACCGAGTCCT GTCCCGGGAT 
CCATCCATGG ACCTGGAGGC AAAGCAGCCG GACTCAGGCA TGTCTTCACC CAACACCACC 
ATGTCGGTCC AGCCGCCGAA CTTTGACCTT GGCTCACCCA CCAGCACCCT CTCCAACTAT 
GACTCCTGCT CCTCCAGCCA TTCCAGCAGC AAGGGTCAGC GCTCTACTCG AGGTGCCAGA 
TCCTCAGACT TACAGAGCTA CATGGACATG CTGAACCCAG AGCCTCGGAG CAAGCAAGGG 
AAGCCTTCAT CTCTACCACC ACCGCCACCA CCAAGCTTCC CTCCACCACC ACCCCCAGGC 
ACACAGCTGC CCCCACCTCC ACCAGGCTAC CCAGCTCCCA ATCCCCCTGT GGGGCTGCAT 
TTGGATAACA TTTACATGCA GACCAAGAAC AAACTCCGCC ACGTGGAGGT GGACTCCCTC 
AAGAAGGAGC CGAGCTCCGG CGACGGCTAC TCGGGGCTAC GCAGGCAGGA TTCCGGGCTG 
CTCAGGCAGG ATTCGGAGCT GCTGCTCAGG CACAACACCG GACTGCGCAG GCAGGACTCC 
GACCGCAAAC AGCGTTCATT CAGTAAACAG CCCAGCACGG GGGACTACTA CCGCCAGCTG 
GGCCGCAGCC CGGGGGAGCC GCTGGCCGCA CGCCCGGGCA TGGCCCACAG CGAGGAGGTG 
CGTGTCCACC AGCCCGCACC TGCGGGCTGC ACAGGATCGA ACCCTGTCTC ACACTCCTCA 
CTCTCTGGCC CCTCTGCTCC CCCGCAGGCG GCGCTGCTCC CCGGGAACCA CGTGCACAAC 
GGCTGCTCCG CGGACTCCAA AGCGTCCAGG GAGCTGCCGC CGCCACCGCC GCCGCCGCCC 
CTGCCCGAGG CCCTGAGTTC GCCGCCACCC GCCCCACCTC TGCCCATCGA GGGCGCGGGC 
GCAGCCTGCG GACAGCGGCG TTCCTCATCT TCTACTGGCA GCACCAAATC TTTCAACATG 
ATGTCCCCAA CGGGTGACAA CTCGGAGTTG CTGGCTGAAA TAAAAGCAGG CAAGAGTCTG 
AAACCAACAC CGCAGAGCAA GGGGCTGACA ACAGTGTTCT CAGGCAGCGG GCAGCCAGCC 
TCCCAGCCTG AGTCACCACA GCCTGCGGTG TCACCTGGGC CATCTCGGGC CCGGAGCCCC 
ACCCCACCAG CCTCTGGGCC TCAGCCACTG CTCAATGGCA GCATAGTACC GGCACCACCT 
GCCACCCTAG CACCAGGAGT GCATCTGGAT GTGGAGGCCC TCATCCCCAC ACTTGATGAG 
CAGGGCCGGC CCATCCCGGA GTGGAAGCGC CAGGTGATGG TCCGGAAGCT GCAGCAGAAA 
ATGCAGGAGG AAGAGGAGCA GCGGAGGAAG GAAGAAGAGG AGGAGGCCCG GCTGGCCAGC 
CTGCCCGCCT GGAGACGAGA CATTCTTAGG AAGAAGCTGG AGGAGGAGAG GGAGCAGAAG 
CGAAAAGAGG AGGAGCGGCA GAAGCTGGAG GAAATCCAGA GGGCGAAAGA ACAGTCGGAG 
AAGCTGCGGA CACTAGGATA CGACGAGGCC AAGCTCGCGC CCTGGCAGCG ACAGGTCATC 
TTGAAGAAGG GGGAGATCCC TAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
The Journal of cell biology212(2)231-44(2016 Jan)
Lelli A,Michel V,Boutet de Monvel J,Cortese M,Bosch-Grau M,Aghaie A,Perfettini I,Dupont T,Avan P,El-Amraoui A,Petit C

Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.
Current biology : CB22(4)320-5(2012 Feb)
Merritt RC,Manor U,Salles FT,Grati M,Dose AC,Unrath WC,Quintero OA,Yengo CM,Kachar B

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Molecular biology of the cell23(2)324-36(2012 Jan)
Revenu C,Ubelmann F,Hurbain I,El-Marjou F,Dingli F,Loew D,Delacour D,Gilet J,Brot-Laroche E,Rivero F,Louvard D,Robine S

Review series: The cell biology of hearing.
The Journal of cell biology190(1)9-20(2010 Jul)
Schwander M,Kachar B,M?ller U

Actin-bundling protein TRIOBP forms resilient rootlets of hair cell stereocilia essential for hearing.
Cell141(5)786-98(2010 May)
Kitajiri S,Sakamoto T,Belyantseva IA,Goodyear RJ,Stepanyan R,Fujiwara I,Bird JE,Riazuddin S,Riazuddin S,Ahmed ZM,Hinshaw JE,Sellers J,Bartles JR,Hammer JA 3rd,Richardson GP,Griffith AJ,Frolenkov GI,Friedman TB

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Espn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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