Uso1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Uso1
Entrez Gene ID	56042
Full Name	USO1 vesicle transport factor
Synonyms	TAP,Vdp
General protein information	Preferred Names USO1 vesicle transport factor Names general vesicular transport factor p115 protein USO1 homolog transcytosis-associated protein vesicle docking protein, 115 kDa vesicle-docking protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	tethering protein involved with transcytotic vesicle transport, including ER to Golgi and between the Golgi stacks [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250750.3	XP_006250812.1	general vesicular transport factor p115 isoform X1
NM_019379.1	NP_062252.1	general vesicular transport factor p115

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Uso1	NP_062363.1
Danio rerio (zebrafish)	uso1	NP_956449.1
Drosophila melanogaster (fruit fly)	p115	NP_572417.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009840	XP_318953.4
Neurospora crassa	NCU01644	XP_956266.1
Canis lupus familiaris (dog)	USO1	XP_005639121.1
Bos taurus (cattle)	USO1	NP_777270.2
Gallus gallus (chicken)	USO1	NP_001026310.1
Caenorhabditis elegans (roundworm)	uso-1	NP_001040974.1
Kluyveromyces lactis	KLLA0E05281g	XP_454183.1
Homo sapiens (human)	USO1	NP_003706.1
Saccharomyces cerevisiae (baker's yeast)	USO1	NP_010225.1
	USO1	XP_001100314.2
Rattus norvegicus (Norway rat)	Uso1	NP_062252.1
Danio rerio (zebrafish)	LOC100334773	XP_002660510.1
Magnaporthe oryzae (rice blast fungus)	MGG_17317	XP_003717804.1
Pan troglodytes (chimpanzee)	USO1	XP_001153352.1
Eremothecium gossypii	AGOS_AER045C	NP_984905.1
Xenopus tropicalis (tropical clawed frog)	uso1	XP_004911135.1

Gene Ontology
Bibliography

Related articles in PubMed

p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles.
Wang T, Grabski R, Sztul E, Hay JC
Traffic (Copenhagen, Denmark)16(2)148-71(2015 Feb)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Golgi disruption and early embryonic lethality in mice lacking USO1.
Kim S, Hill A, Warman ML, Smits P
PloS one7(11)e50530(2012)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Monomeric SNAREs are a major binding site for p115 on COPII vesicles, and that p115 dissociates from its SNARE partners upon SNAREpin assembly.
Title: p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles.

Golgi organization depends on mutually interacting domains in betaCOP and p115, suggesting that vesicle tethering at the Golgi involves p115 binding to the COPI coat
Title: Coat-tether interaction in Golgi organization.

Regulation of this interaction by proteins such as GM130 and Giantin may control the membrane recruitment of p115 by Rab1.
Title: A cryptic Rab1-binding site in the p115 tethering protein.

p115 coordinates sequential tethering and docking of COPI vesicles by first using long tethers (Golgins) and then short tethers (SNAREs).
Title: Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115.

The following Uso1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Uso1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04610	NM_019379.1 Latest version!	Rattus norvegicus USO1 vesicle transport factor (Uso1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$566.30 $809.00
ORa35914	XM_006250750.3 Latest version!	Rattus norvegicus USO1 vesicle transport factor (Uso1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Uso1 ( NM_019379.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062252.1 Rattus norvegicus USO1 vesicle transport factor (Uso1), mRNA.

Price & Availability↑

CloneID	ORa04610
Clone ID Related Accession (Same CDS sequence)	NM_019379.1
Accession Version	NM_019379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	general vesicular transport factor p115
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U15589.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U15589.1, U14192.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAATTTCC TCCGCGGGGT GATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCT 
GAGACGATTC AGAAGCTCTG TGACCGCGTA GCTTCATCTA CTTTACTGGA CGACCGAAGA 
AATGCTGTCC GTGCCCTTAA GTCACTGTCT AAGAAATACC GCTTGGAAGT GGGAATCCAA 
GCCATGGAGC ATCTTATCCA CGTCTTACAG ACAGATCGTT CGGATTCTGA AATAATAGCG 
TATGCTTTGG ACACACTCTA TAATATAATA TCCAATGATG AAGAGGAAGA AGTAGAAGAA 
AATTCTACAA GACAGAGTGA GGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CCAGAAAATG TCACTCTCCT GTTATCTTTG TTGGAGGAGT TTGATTTCCA CGTCCGATGG 
CCTGGTGTGA GACTTCTGAC TTCTCTTTTA AAACAGCTAG GGCCTCCAGT GCAGCAAATC 
ATCCTAGTCA GCCCCATGGG TGTTTCAAAA TTAATGGACT TGCTGGCAGA TTCCAGGGAA 
ATTATACGTA ATGATGGCGT CTTACTACTG CAGGCATTAA CAAGGAGCAA CGGAGCGATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TACAGAGGAG 
GGGAACAGCG ATGGAGGTAT AGTAGTGGAA GATTGTTTGA TTTTGCTCCA AAATTTGTTA 
AAAAACAACA ATTCCAATCA AAATTTTTTT AAAGAAGGCT CTTATATTCA ACGTATGAAA 
GCTTGGTTTG AAGTTGGAGA TGAAAATCCT GGTTGGTCAG CACAGAAAGT GACCAATCTT 
CATTTAATGC TCCAGCTTGT GCGGGTACTG GTGTCTCCCA CCAACCCTCC CGGTGCTACC 
AGTAGCTGCC AGAAGGCCAT GTTCCAGTGT GGGTTACTAC AACAGCTTTG CACTATCCTG 
ATGGCTACCG GAATTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA TCAAGACTAC TTTGCTTCTG TGAATGCGCC TTCAAATCCC 
CCACGACCGG CAATCGTTGT GCTGCTCATG TCCATGGTCA ACGAGAGGCA GCCATTTGTA 
CTGCGCTGCG CCGTGCTCTA CTGTTTCCAG TGCTTCCTCT ATAAAAACGA GAAAGGACAA 
GGAGAGATTG TGGCCACTCT GCTACCTTCC ACTATTGACG CAACAGGTAA CTCGGTCTCA 
GCTGGCCAGC TGCTCTGCGG AGGTTTGTTT TCCACAGACT CCCTCTCAAA CTGGTGTGCT 
GCCGTGGCCC TCGCCCATGC ACTGCAAGGG AACGCCACCC AGAAGGAGCA GCTGCTCAGG 
GTTCAGCTGG CCACCAGCAT CGGCAACCCC CCAGTGTCGC TCTTGCAGCA GTGCACCAAC 
ATTCTGTCCC AGGGAAGCAA GATACAGACA AGAGTCGGGT TGTTGATGTT GCTTTGTACC 
TGGTTAAGCA ACTGTCCGAT TGCTGTAACA CACTTTCTTC ACAACTCAGC CAATGTTCCA 
TTTCTTACAG GACAGATTGC AGAAAATCTC GGAGAAGAAG AGCAGTTGGT CCAAGGCTTA 
TGTGCCCTTC TTTTGGGCAT TTCAATTTAT TTCAACGACA ACTCACTAGA AAACTACACG 
AAAGAGAAAC TAAAGCAACT AATAGAGAAG AGGATTGGCA AAGAGAATTA CATAGAGAAA 
CTTGGATTTA TTAGCAAGCA TGAGTTATAT CCCAGAGCAT CTCAGAAACC CCAGCCAAAC 
TTTCCGAGTC CAGAATACAT GATATTTGAT CATGAGTTTA CAAAACTGGT GAAAGAACTT 
GAAGGTGTTA TTACCAAGGC TATTTATAAG TCCAGCGAAG AAGATAAGAA AGAGGAAGAG 
GTAAAGAAAA CCTTAGAACA GCATGATAAT ATTGTGACTC ACTATAAGAA TGTGATCCGT 
GAGCAAGACC TACAACTGGA GGAACTAAAA CAGCAAGTGT CCACACTGAA ATGTCAGAAC 
GAGCAGCTGC AAACAGCAGT CACCCAGCAG GCTTCTCAGA TTCAGCAGCA CAAGGATCAG 
TACAACCTCC TCAAAGTTCA GCTCGGGAAA GACAATCACC ATCAAGGTTC TCACAGTGAC 
GGGGCTCAGG TAAATGGCAT TCAGCCAGAG GAAATCAGTC GGCTGAGGGA AGAGATAGAA 
GAATTAAGAA GTCATCAGGT GCTCTTACAG AGCCAGCTAG CTGAAAAGGA CACTGTGATT 
GAAAATTTGA GATCTTCACA AGTGTCTGGC ATGAGTGAAC AGGCTTTAGC AACGTGTTCA 
CCCAGAGATG CCGAGCAAGT TGCAGAGTTA AAACAGGAAT TGTCAGCATT AAAGTCCCAG 
TTATGTTCAC AGTCTCTGGA GATCACTAGA CTCCAGACAG AGAACCGTGA GCTGCAACAG 
AGAGCAGAGA CCTTGGCAAA GTCAGTTCCT GTAGAAGGAG AGAGTGAGCT TGTGACAGCC 
GCAAAAACTA CGGATGTAGA GGGAAGGCTG TCTGCCCTGC TGCAGGAGAC CAAAGAGTTA 
AAGAATGAGA TTAAAGCATT GTCTGAGGAG AGGACCTCCA TTCAAAAGCA GCTGGACTCC 
TCCAACAGCA CCATCGCCAT CCTACAAACG GAGAAAGACA AGCTATACTT GGAAGTTACG 
GATTCTAAGA AAGAACAAGA TGATCTTTTG GTGCTGTTGG CAGATCAAGA TCAGAAAATC 
CTGTCACTGA AGAGTAAACT CAAGGATCTT GGTCATCCAG TTGAAGAAGA AGATGAATCT 
GGAGACCAAG AAGATGATGA TGATGAACTT GACGATGGTG ACAGGGACCA GGATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062252.1
CDS	97..2976
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIAYALDTLYNIISNDEEEEVEENSTRQSEDLGSQFTEIFIKQPENVTLLLSLLEEFDFHVRWPGVRLLTSLLKQLGPPVQQIILVSPMGVSKLMDLLADSREIIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIITEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKAWFEVGDENPGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGIPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNEKGQGEIVATLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQGNATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLENYTKEKLKQLIEKRIGKENYIEKLGFISKHELYPRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNVIREQDLQLEELKQQVSTLKCQNEQLQTAVTQQASQIQQHKDQYNLLKVQLGKDNHHQGSHSDGAQVNGIQPEEISRLREEIEELRSHQVLLQSQLAEKDTVIENLRSSQVSGMSEQALATCSPRDAEQVAELKQELSALKSQLCSQSLEITRLQTENRELQQRAETLAKSVPVEGESELVTAAKTTDVEGRLSALLQETKELKNEIKALSEERTSIQKQLDSSNSTIAILQTEKDKLYLEVTDSKKEQDDLLVLLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDDELDDGDRDQDI

Target ORF information:

RefSeq Version	NM_019379.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus USO1 vesicle transport factor (Uso1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019379.1

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT GATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCT 
GAGACGATTC AGAAGCTCTG TGACCGCGTA GCTTCATCTA CTTTACTGGA CGACCGAAGA 
AATGCTGTCC GTGCCCTTAA GTCACTGTCT AAGAAATACC GCTTGGAAGT GGGAATCCAA 
GCCATGGAGC ATCTTATCCA CGTCTTACAG ACAGATCGTT CGGATTCTGA AATAATAGCG 
TATGCTTTGG ACACACTCTA TAATATAATA TCCAATGATG AAGAGGAAGA AGTAGAAGAA 
AATTCTACAA GACAGAGTGA GGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CCAGAAAATG TCACTCTCCT GTTATCTTTG TTGGAGGAGT TTGATTTCCA CGTCCGATGG 
CCTGGTGTGA GACTTCTGAC TTCTCTTTTA AAACAGCTAG GGCCTCCAGT GCAGCAAATC 
ATCCTAGTCA GCCCCATGGG TGTTTCAAAA TTAATGGACT TGCTGGCAGA TTCCAGGGAA 
ATTATACGTA ATGATGGCGT CTTACTACTG CAGGCATTAA CAAGGAGCAA CGGAGCGATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TACAGAGGAG 
GGGAACAGCG ATGGAGGTAT AGTAGTGGAA GATTGTTTGA TTTTGCTCCA AAATTTGTTA 
AAAAACAACA ATTCCAATCA AAATTTTTTT AAAGAAGGCT CTTATATTCA ACGTATGAAA 
GCTTGGTTTG AAGTTGGAGA TGAAAATCCT GGTTGGTCAG CACAGAAAGT GACCAATCTT 
CATTTAATGC TCCAGCTTGT GCGGGTACTG GTGTCTCCCA CCAACCCTCC CGGTGCTACC 
AGTAGCTGCC AGAAGGCCAT GTTCCAGTGT GGGTTACTAC AACAGCTTTG CACTATCCTG 
ATGGCTACCG GAATTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA TCAAGACTAC TTTGCTTCTG TGAATGCGCC TTCAAATCCC 
CCACGACCGG CAATCGTTGT GCTGCTCATG TCCATGGTCA ACGAGAGGCA GCCATTTGTA 
CTGCGCTGCG CCGTGCTCTA CTGTTTCCAG TGCTTCCTCT ATAAAAACGA GAAAGGACAA 
GGAGAGATTG TGGCCACTCT GCTACCTTCC ACTATTGACG CAACAGGTAA CTCGGTCTCA 
GCTGGCCAGC TGCTCTGCGG AGGTTTGTTT TCCACAGACT CCCTCTCAAA CTGGTGTGCT 
GCCGTGGCCC TCGCCCATGC ACTGCAAGGG AACGCCACCC AGAAGGAGCA GCTGCTCAGG 
GTTCAGCTGG CCACCAGCAT CGGCAACCCC CCAGTGTCGC TCTTGCAGCA GTGCACCAAC 
ATTCTGTCCC AGGGAAGCAA GATACAGACA AGAGTCGGGT TGTTGATGTT GCTTTGTACC 
TGGTTAAGCA ACTGTCCGAT TGCTGTAACA CACTTTCTTC ACAACTCAGC CAATGTTCCA 
TTTCTTACAG GACAGATTGC AGAAAATCTC GGAGAAGAAG AGCAGTTGGT CCAAGGCTTA 
TGTGCCCTTC TTTTGGGCAT TTCAATTTAT TTCAACGACA ACTCACTAGA AAACTACACG 
AAAGAGAAAC TAAAGCAACT AATAGAGAAG AGGATTGGCA AAGAGAATTA CATAGAGAAA 
CTTGGATTTA TTAGCAAGCA TGAGTTATAT CCCAGAGCAT CTCAGAAACC CCAGCCAAAC 
TTTCCGAGTC CAGAATACAT GATATTTGAT CATGAGTTTA CAAAACTGGT GAAAGAACTT 
GAAGGTGTTA TTACCAAGGC TATTTATAAG TCCAGCGAAG AAGATAAGAA AGAGGAAGAG 
GTAAAGAAAA CCTTAGAACA GCATGATAAT ATTGTGACTC ACTATAAGAA TGTGATCCGT 
GAGCAAGACC TACAACTGGA GGAACTAAAA CAGCAAGTGT CCACACTGAA ATGTCAGAAC 
GAGCAGCTGC AAACAGCAGT CACCCAGCAG GCTTCTCAGA TTCAGCAGCA CAAGGATCAG 
TACAACCTCC TCAAAGTTCA GCTCGGGAAA GACAATCACC ATCAAGGTTC TCACAGTGAC 
GGGGCTCAGG TAAATGGCAT TCAGCCAGAG GAAATCAGTC GGCTGAGGGA AGAGATAGAA 
GAATTAAGAA GTCATCAGGT GCTCTTACAG AGCCAGCTAG CTGAAAAGGA CACTGTGATT 
GAAAATTTGA GATCTTCACA AGTGTCTGGC ATGAGTGAAC AGGCTTTAGC AACGTGTTCA 
CCCAGAGATG CCGAGCAAGT TGCAGAGTTA AAACAGGAAT TGTCAGCATT AAAGTCCCAG 
TTATGTTCAC AGTCTCTGGA GATCACTAGA CTCCAGACAG AGAACCGTGA GCTGCAACAG 
AGAGCAGAGA CCTTGGCAAA GTCAGTTCCT GTAGAAGGAG AGAGTGAGCT TGTGACAGCC 
GCAAAAACTA CGGATGTAGA GGGAAGGCTG TCTGCCCTGC TGCAGGAGAC CAAAGAGTTA 
AAGAATGAGA TTAAAGCATT GTCTGAGGAG AGGACCTCCA TTCAAAAGCA GCTGGACTCC 
TCCAACAGCA CCATCGCCAT CCTACAAACG GAGAAAGACA AGCTATACTT GGAAGTTACG 
GATTCTAAGA AAGAACAAGA TGATCTTTTG GTGCTGTTGG CAGATCAAGA TCAGAAAATC 
CTGTCACTGA AGAGTAAACT CAAGGATCTT GGTCATCCAG TTGAAGAAGA AGATGAATCT 
GGAGACCAAG AAGATGATGA TGATGAACTT GACGATGGTG ACAGGGACCA GGATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Uso1 ( XM_006250750.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250812.1 Rattus norvegicus USO1 vesicle transport factor (Uso1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35914
Clone ID Related Accession (Same CDS sequence)	XM_006250750.3
Accession Version	XM_006250750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	general vesicular transport factor p115 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTCC TCCGCGGGGT GATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCT 
GAGACGATTC AGAAGCTCTG TGACCGCGTA GCTTCATCTA CTTTACTGGA CGACCGAAGA 
AATGCTGTCC GTGCCCTTAA GTCACTGTCT AAGAAATACC GCTTGGAAGT GGGAATCCAA 
GCCATGGAGC ATCTTATCCA CGTCTTACAG ACAGATCGTT CGGATTCTGA AATAATAGCG 
TATGCTTTGG ACACACTCTA TAATATAATA TCCAATGATG AAGAGGAAGA AGTAGAAGAA 
AATTCTACAA GACAGAGTGA GGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CCAGAAAATG TCACTCTCCT GTTATCTTTG TTGGAGGAGT TTGATTTCCA CGTCCGATGG 
CCTGGTGTGA GACTTCTGAC TTCTCTTTTA AAACAGCTAG GGCCTCCAGT GCAGCAAATC 
ATCCTAGTCA GCCCCATGGG TGTTTCAAAA TTAATGGACT TGCTGGCAGA TTCCAGGGAA 
ATTATACGTA ATGATGGCGT CTTACTACTG CAGGCATTAA CAAGGAGCAA CGGAGCGATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TACAGAGGAG 
GGGAACAGCG ATGGAGGTAT AGTAGTGGAA GATTGTTTGA TTTTGCTCCA AAATTTGTTA 
AAAAACAACA ATTCCAATCA AAATTTTTTT AAAGAAGGCT CTTATATTCA ACGTATGAAA 
GCTTGGTTTG AAGTTGGAGA TGAAAATCCT GGTTGGTCAG CACAGAAAGT GACCAATCTT 
CATTTAATGC TCCAGCTTGT GCGGGTACTG GTGTCTCCCA CCAACCCTCC CGGTGCTACC 
AGTAGCTGCC AGAAGGCCAT GTTCCAGTGT GGGTTACTAC AACAGCTTTG CACTATCCTG 
ATGGCTACCG GAATTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA TCAAGACTAC TTTGCTTCTG TGAATGCGCC TTCAAATCCC 
CCACGACCGG CAATCGTTGT GCTGCTCATG TCCATGGTCA ACGAGAGGCA GCCATTTGTA 
CTGCGCTGCG CCGTGCTCTA CTGTTTCCAG TGCTTCCTCT ATAAAAACGA GAAAGGACAA 
GGAGAGATTG TGGCCACTCT GCTACCTTCC ACTATTGACG CAACAGGTAA CTCGGTCTCA 
GCTGGCCAGC TGCTCTGCGG AGGTTTGTTT TCCACAGACT CCCTCTCAAA CTGGTGTGCT 
GCCGTGGCCC TCGCCCATGC ACTGCAAGGG AACGCCACCC AGAAGGAGCA GCTGCTCAGG 
GTTCAGCTGG CCACCAGCAT CGGCAACCCC CCAGTGTCGC TCTTGCAGCA GTGCACCAAC 
ATTCTGTCCC AGGGCGATAA GATCGACAGA CGGGGAAGCA AGATACAGAC AAGAGTCGGG 
TTGTTGATGT TGCTTTGTAC CTGGTTAAGC AACTGTCCGA TTGCTGTAAC ACACTTTCTT 
CACAACTCAG CCAATGTTCC ATTTCTTACA GGACAGATTG CAGAAAATCT CGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT CTTTTGGGCA TTTCAATTTA TTTCAACGAC 
AACTCACTAG AAAACTACAC GAAAGAGAAA CTAAAGCAAC TAATAGAGAA GAGGATTGGC 
AAAGAGAATT ACATAGAGAA ACTTGGATTT ATTAGCAAGC ATGAGTTATA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCGAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACAAAACTGG TGAAAGAACT TGAAGGTGTT ATTACCAAGG CTATTTATAA GTCCAGCGAA 
GAAGATAAGA AAGAGGAAGA GGTAAAGAAA ACCTTAGAAC AGCATGATAA TATTGTGACT 
CACTATAAGA ATATGATCCG TGAGCAAGAC CTACAACTGG AGGAACTAAA ACAGCAAGTG 
TCCACACTGA AATGTCAGAA CGAGCAGCTG CAAACAGCAG TCACCCAGCA GGCTTCTCAG 
ATTCAGCAGC ACAAGGATCA GTACAACCTC CTCAAAGTTC AGCTCGGGAA AGACAATCAC 
CATCAAGGTT CTCACAGTGA CGGGGCTCAG GTAAATGGCA TTCAGCCAGA GGAAATCAGT 
CGGCTGAGGG AAGAGATAGA AGAATTAAGA AGTCATCAGG TGCTCTTACA GAGCCAGCTA 
GCTGAAAAGG ACACTGTGAT TGAAAATTTG AGATCTTCAC AAGTGTCTGG CATGAGTGAA 
CAGGCTTTAG CAACGTGTTC ACCCAGAGAT GCCGAGCAAG TTGCAGAGTT AAAACAGGAA 
TTGTCAGCAT TAAAGTCCCA GTTATGTTCA CAGTCTCTGG AGATCACTAG ACTCCAGACA 
GAGAACAGTG AGCTGCAACA GAGAGCAGAG ACCTTGGCAA AGTCAGTTCC TGTAGAAGGA 
GAGAGTGAGC TTGTGACAGC CGCAAAAACT ACGGATGTAG AGGGAAGGCT GTCTGCCCTG 
CTGCAGGAGA CCAAAGAGTT AAAGAATGAG ATTAAAGCAT TGTCTGAGGA GAGGACCGCC 
ATTCAAAAGC AGCTGGACTC CTCCAACAGC ACCATCGCCA TCCTACAAAC GGAGAAAGAC 
AAGCTATACT TGGAAGTTAC GGATTCTAAG AAAGAACAAG ATGATCTTTT GGTGCTGTTG 
GCAGATCAAG ATCAGAAAAT CCTGTCACTG AAGAGTAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AAGATGAATC TGGAGACCAA GAAGATGATG ATGATGAACT TGACGATGGT 
GACAGGGACC AGGATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250812.1
CDS	148..3048
Translation	MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQTDRSDSEIIAYALDTLYNIISNDEEEEVEENSTRQSEDLGSQFTEIFIKQPENVTLLLSLLEEFDFHVRWPGVRLLTSLLKQLGPPVQQIILVSPMGVSKLMDLLADSREIIRNDGVLLLQALTRSNGAIQKIVAFENAFERLLDIITEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKAWFEVGDENPGWSAQKVTNLHLMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGIPADILTETINTVSEVIRGCQVNQDYFASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNEKGQGEIVATLLPSTIDATGNSVSAGQLLCGGLFSTDSLSNWCAAVALAHALQGNATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGDKIDRRGSKIQTRVGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLENYTKEKLKQLIEKRIGKENYIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKSSEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELKQQVSTLKCQNEQLQTAVTQQASQIQQHKDQYNLLKVQLGKDNHHQGSHSDGAQVNGIQPEEISRLREEIEELRSHQVLLQSQLAEKDTVIENLRSSQVSGMSEQALATCSPRDAEQVAELKQELSALKSQLCSQSLEITRLQTENSELQQRAETLAKSVPVEGESELVTAAKTTDVEGRLSALLQETKELKNEIKALSEERTAIQKQLDSSNSTIAILQTEKDKLYLEVTDSKKEQDDLLVLLADQDQKILSLKSKLKDLGHPVEEEDESGDQEDDDDELDDGDRDQDI

Target ORF information:

RefSeq Version	XM_006250750.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus USO1 vesicle transport factor (Uso1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250750.3

ORF Insert Sequence:

ATGAATTTCC TCCGCGGGGT GATGGGGGGT CAGAGTGCCG GACCCCAGCA CACAGAAGCT 
GAGACGATTC AGAAGCTCTG TGACCGCGTA GCTTCATCTA CTTTACTGGA CGACCGAAGA 
AATGCTGTCC GTGCCCTTAA GTCACTGTCT AAGAAATACC GCTTGGAAGT GGGAATCCAA 
GCCATGGAGC ATCTTATCCA CGTCTTACAG ACAGATCGTT CGGATTCTGA AATAATAGCG 
TATGCTTTGG ACACACTCTA TAATATAATA TCCAATGATG AAGAGGAAGA AGTAGAAGAA 
AATTCTACAA GACAGAGTGA GGATTTGGGA AGCCAATTTA CAGAAATTTT CATTAAGCAG 
CCAGAAAATG TCACTCTCCT GTTATCTTTG TTGGAGGAGT TTGATTTCCA CGTCCGATGG 
CCTGGTGTGA GACTTCTGAC TTCTCTTTTA AAACAGCTAG GGCCTCCAGT GCAGCAAATC 
ATCCTAGTCA GCCCCATGGG TGTTTCAAAA TTAATGGACT TGCTGGCAGA TTCCAGGGAA 
ATTATACGTA ATGATGGCGT CTTACTACTG CAGGCATTAA CAAGGAGCAA CGGAGCGATC 
CAGAAAATTG TTGCTTTTGA AAATGCTTTC GAGAGACTAC TGGACATTAT TACAGAGGAG 
GGGAACAGCG ATGGAGGTAT AGTAGTGGAA GATTGTTTGA TTTTGCTCCA AAATTTGTTA 
AAAAACAACA ATTCCAATCA AAATTTTTTT AAAGAAGGCT CTTATATTCA ACGTATGAAA 
GCTTGGTTTG AAGTTGGAGA TGAAAATCCT GGTTGGTCAG CACAGAAAGT GACCAATCTT 
CATTTAATGC TCCAGCTTGT GCGGGTACTG GTGTCTCCCA CCAACCCTCC CGGTGCTACC 
AGTAGCTGCC AGAAGGCCAT GTTCCAGTGT GGGTTACTAC AACAGCTTTG CACTATCCTG 
ATGGCTACCG GAATTCCTGC TGATATCCTG ACTGAGACCA TAAATACTGT ATCAGAAGTT 
ATTCGAGGCT GCCAAGTAAA TCAAGACTAC TTTGCTTCTG TGAATGCGCC TTCAAATCCC 
CCACGACCGG CAATCGTTGT GCTGCTCATG TCCATGGTCA ACGAGAGGCA GCCATTTGTA 
CTGCGCTGCG CCGTGCTCTA CTGTTTCCAG TGCTTCCTCT ATAAAAACGA GAAAGGACAA 
GGAGAGATTG TGGCCACTCT GCTACCTTCC ACTATTGACG CAACAGGTAA CTCGGTCTCA 
GCTGGCCAGC TGCTCTGCGG AGGTTTGTTT TCCACAGACT CCCTCTCAAA CTGGTGTGCT 
GCCGTGGCCC TCGCCCATGC ACTGCAAGGG AACGCCACCC AGAAGGAGCA GCTGCTCAGG 
GTTCAGCTGG CCACCAGCAT CGGCAACCCC CCAGTGTCGC TCTTGCAGCA GTGCACCAAC 
ATTCTGTCCC AGGGCGATAA GATCGACAGA CGGGGAAGCA AGATACAGAC AAGAGTCGGG 
TTGTTGATGT TGCTTTGTAC CTGGTTAAGC AACTGTCCGA TTGCTGTAAC ACACTTTCTT 
CACAACTCAG CCAATGTTCC ATTTCTTACA GGACAGATTG CAGAAAATCT CGGAGAAGAA 
GAGCAGTTGG TCCAAGGCTT ATGTGCCCTT CTTTTGGGCA TTTCAATTTA TTTCAACGAC 
AACTCACTAG AAAACTACAC GAAAGAGAAA CTAAAGCAAC TAATAGAGAA GAGGATTGGC 
AAAGAGAATT ACATAGAGAA ACTTGGATTT ATTAGCAAGC ATGAGTTATA TTCCAGAGCA 
TCTCAGAAAC CCCAGCCAAA CTTTCCGAGT CCAGAATACA TGATATTTGA TCATGAGTTT 
ACAAAACTGG TGAAAGAACT TGAAGGTGTT ATTACCAAGG CTATTTATAA GTCCAGCGAA 
GAAGATAAGA AAGAGGAAGA GGTAAAGAAA ACCTTAGAAC AGCATGATAA TATTGTGACT 
CACTATAAGA ATATGATCCG TGAGCAAGAC CTACAACTGG AGGAACTAAA ACAGCAAGTG 
TCCACACTGA AATGTCAGAA CGAGCAGCTG CAAACAGCAG TCACCCAGCA GGCTTCTCAG 
ATTCAGCAGC ACAAGGATCA GTACAACCTC CTCAAAGTTC AGCTCGGGAA AGACAATCAC 
CATCAAGGTT CTCACAGTGA CGGGGCTCAG GTAAATGGCA TTCAGCCAGA GGAAATCAGT 
CGGCTGAGGG AAGAGATAGA AGAATTAAGA AGTCATCAGG TGCTCTTACA GAGCCAGCTA 
GCTGAAAAGG ACACTGTGAT TGAAAATTTG AGATCTTCAC AAGTGTCTGG CATGAGTGAA 
CAGGCTTTAG CAACGTGTTC ACCCAGAGAT GCCGAGCAAG TTGCAGAGTT AAAACAGGAA 
TTGTCAGCAT TAAAGTCCCA GTTATGTTCA CAGTCTCTGG AGATCACTAG ACTCCAGACA 
GAGAACAGTG AGCTGCAACA GAGAGCAGAG ACCTTGGCAA AGTCAGTTCC TGTAGAAGGA 
GAGAGTGAGC TTGTGACAGC CGCAAAAACT ACGGATGTAG AGGGAAGGCT GTCTGCCCTG 
CTGCAGGAGA CCAAAGAGTT AAAGAATGAG ATTAAAGCAT TGTCTGAGGA GAGGACCGCC 
ATTCAAAAGC AGCTGGACTC CTCCAACAGC ACCATCGCCA TCCTACAAAC GGAGAAAGAC 
AAGCTATACT TGGAAGTTAC GGATTCTAAG AAAGAACAAG ATGATCTTTT GGTGCTGTTG 
GCAGATCAAG ATCAGAAAAT CCTGTCACTG AAGAGTAAAC TCAAGGATCT TGGTCATCCA 
GTTGAAGAAG AAGATGAATC TGGAGACCAA GAAGATGATG ATGATGAACT TGACGATGGT 
GACAGGGACC AGGATATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles.
Traffic (Copenhagen, Denmark)16(2)148-71(2015 Feb)
Wang T,Grabski R,Sztul E,Hay JC

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Golgi disruption and early embryonic lethality in mice lacking USO1.
PloS one7(11)e50530(2012)
Kim S,Hill A,Warman ML,Smits P

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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オンライン注文

Uso1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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