Egln3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egln3
Entrez Gene ID	54702
Full Name	egl-9 family hypoxia-inducible factor 3
Synonyms	PHD-3,PHD3,SM-20
General protein information	Preferred Names egl-9 family hypoxia-inducible factor 3 Names egl nine homolog 3 HIF-PH3 HIF-prolyl hydroxylase 3 HPH-3 egl nine homolog 3, mitochondrial factor-responsive smooth muscle protein hypoxia-inducible factor prolyl hydroxylase 3 prolyl hydroxylase domain-containing protein 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q23
Summary	plays a role in regulation of cell proliferation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019371.1	NP_062244.1	egl nine homolog 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	EGLN3	NP_071356.1
Xenopus tropicalis (tropical clawed frog)	egln3	NP_001120484.1
	EGLN3	XP_002805061.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003523	XP_313264.5
Rattus norvegicus (Norway rat)	Egln3	NP_062244.1
Pan troglodytes (chimpanzee)	EGLN3	XP_509896.3
Bos taurus (cattle)	EGLN3	NP_001094634.1
Danio rerio (zebrafish)	egln3	NP_998475.1
Canis lupus familiaris (dog)	EGLN3	XP_537408.3
Mus musculus (house mouse)	Egln3	NP_082409.2
Gallus gallus (chicken)	EGLN3	XP_421233.3

Gene Ontology
Bibliography

Related articles in PubMed

Changes in Hypoxia-Inducible Factor-1 (HIF-1) and Regulatory Prolyl Hydroxylase (PHD) Enzymes Following Hypoxic-Ischemic Injury in the Neonatal Rat.
Chu HX, Jones NM
Neurochemical research41(3)515-22(2016 Mar)

Depletion of PHD3 protects heart from ischemia/reperfusion injury by inhibiting cardiomyocyte apoptosis.
Xie L, Pi X, Wang Z, He J, Willis MS, Patterson C
Journal of molecular and cellular cardiology80156-65(2015 Mar)

Advanced glycated end-products affect HIF-transcriptional activity in renal cells.
Bondeva T, Heinzig J, Ruhe C, Wolf G
Molecular endocrinology (Baltimore, Md.)27(11)1918-33(2013 Nov)

Prospective study assessing hypoxia-related proteins as markers for the outcome of treatment with sunitinib in advanced clear-cell renal cell carcinoma.
Garcia-Donas J, Leandro-Garc?a LJ, Gonz?lez Del Alba A, Morente M, Alemany I, Esteban E, Arranz JA, Climent MA, Gallardo E, Castellano DE, Bellmunt J, Mellado B, Puente J, Moreno F, Font A, Hernando S, Robledo M, Rodr?guez-Antona C
Annals of oncology : official journal of the European Society for Medical Oncology24(9)2409-14(2013 Sep)

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Laitala A, Aro E, Walkinshaw G, M?ki JM, Rossi M, Heikkil? M, Savolainen ER, Arend M, Kivirikko KI, Koivunen P, Myllyharju J
Blood120(16)3336-44(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

24 h after HI, there was a significant increase in PHD3 protein and an increase of HIF-1alpha compared to controls
Title: Changes in Hypoxia-Inducible Factor-1 (HIF-1) and Regulatory Prolyl Hydroxylase (PHD) Enzymes Following Hypoxic-Ischemic Injury in the Neonatal Rat.

depletion of PHD3 leads to increased stabilization of HIF-1alpha and inhibition of DNA damage response, both of which may contribute to the cardioprotective effect seen with depletion of PHD3.
Title: Depletion of PHD3 protects heart from ischemia/reperfusion injury by inhibiting cardiomyocyte apoptosis.

Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis factor-alpha (TNF-alpha) on cells of the nucleus pulposus through co-activation of nuclear factor kappaB (NF-kappaB)/p65 signaling.
Title: Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis factor-? (TNF-?) on cells of the nucleus pulposus through co-activation of nuclear factor ?B (NF-?B)/p65 signaling.

Egln3 suppresses glioma progression
Title: Suppression of glioma progression by Egln3.

PHD3 upregulation may be partially responsible for DOX-induced cardiomyocyte apoptosis via its interaction with Bcl-2.
Title: Prolyl hydroxylase 3 interacts with Bcl-2 to regulate doxorubicin-induced apoptosis in H9c2 cells.

The following Egln3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egln3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04591	NM_019371.1 Latest version!	Rattus norvegicus egl-9 family hypoxia-inducible factor 3 (Egln3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egln3 ( NM_019371.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062244.1 Rattus norvegicus egl-9 family hypoxia-inducible factor 3 (Egln3), mRNA.

Price & Availability↑

CloneID	ORa04591
Clone ID Related Accession (Same CDS sequence)	NM_019371.1
Accession Version	NM_019371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	egl nine homolog 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U06713.1. On Jan 12, 2005 this sequence version replaced XM_346767.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U06713.1, FM119740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACGTTGA GGTCAAGGCG GGGCTTCCTC TCGTTCCTCC CCGGGCTGCG GCCGCCGCGG 
CGGTGGCTCC GCATCTCCAA AAGGGGCCCT CCGACTTCTC ACTGGGCCAG TCCGGCGCTG 
GGTGGCAGAA CTCTACACTA CTCCTGCCGG TCTCAGAGCG GGACTCCCTT CTCTTCAGAG 
TTTCAAGCAA CTTTCCCCGC CTTTGCAGCT AAAGTGGCTC GGGGACCGTG GCTGCCGCAG 
GTGGTAGAGC CCCCGGCCCG GCTCTCTGCA TCTCCGCTGT GCGTTCGCTC TGGGCAAGCC 
CTCGGTGCGT GCACCCTGGG GGTCCCCCGC CTCGGCTCTG TGAGCGAGAT GCCTCTGGGA 
CACATCATGA GGCTGGATCT GGAGAAGATC GCCCTGGAGT ACATCGTGCC CTGTCTGCAC 
GAGGTCGGTT TCTGCTACCT GGACAACTTC CTAGGCGAGG TGGTGGGCGA CTGCGTACTG 
GAGCGAGTCA AGCAGCTGCA TTACAACGGG GCCCTGCGTG ACGGCCAGCT GGCGGGGCCC 
CGCGCCGGCG TCTCCAAGCG GCACCTGCGA GGCGACCAGA TCACGTGGAT CGGGGGCAAC 
GAGGAGGGCT GCGAGGCCAT CAACTTCCTC CTGTCCCTCA TCGACAGGCT GGTCCTGTAC 
TGCGGGAGCC GCCTGGGCAA ATACTATGTC AAGGAGCGGT CCAAGGCGAT GGTGGCTTGC 
TATCCAGGAA ATGGAACAGG TTATGTTCGT CATGTGGACA ACCCCAATGG TGATGGCCGC 
TGTATCACCT GTATCTACTA CCTGAATAAG AATTGGGACG CCAAGTTACA TGGAGGGGTC 
CTGCGGATAT TTCCAGAAGG AAAATCATTT GTAGCAGACG TGGAGCCCAT TTTTGATAGA 
CTCCTGTTCT CCTGGTCAGA CCGCAGGAAT CCACATGAAG TTCAGCCCTC CTATGCCACC 
AGGTACGCCA TGACTGTCTG GTACTTCGAT GCTGAAGAAA GGGCAGAAGC CAAAAAGAAA 
TTCAGGAATC TAACTAGAAA AACTGAATCT GCTCTTGCTA AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062244.1
CDS	191..1258
Translation	MTLRSRRGFLSFLPGLRPPRRWLRISKRGPPTSHWASPALGGRTLHYSCRSQSGTPFSSEFQATFPAFAAKVARGPWLPQVVEPPARLSASPLCVRSGQALGACTLGVPRLGSVSEMPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHYNGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAINFLLSLIDRLVLYCGSRLGKYYVKERSKAMVACYPGNGTGYVRHVDNPNGDGRCITCIYYLNKNWDAKLHGGVLRIFPEGKSFVADVEPIFDRLLFSWSDRRNPHEVQPSYATRYAMTVWYFDAEERAEAKKKFRNLTRKTESALAKD

Target ORF information:

RefSeq Version	NM_019371.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus egl-9 family hypoxia-inducible factor 3 (Egln3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019371.1

ORF Insert Sequence:

ATGACGTTGA GGTCAAGGCG GGGCTTCCTC TCGTTCCTCC CCGGGCTGCG GCCGCCGCGG 
CGGTGGCTCC GCATCTCCAA AAGGGGCCCT CCGACTTCTC ACTGGGCCAG TCCGGCGCTG 
GGTGGCAGAA CTCTACACTA CTCCTGCCGG TCTCAGAGCG GGACTCCCTT CTCTTCAGAG 
TTTCAAGCAA CTTTCCCCGC CTTTGCAGCT AAAGTGGCTC GGGGACCGTG GCTGCCGCAG 
GTGGTAGAGC CCCCGGCCCG GCTCTCTGCA TCTCCGCTGT GCGTTCGCTC TGGGCAAGCC 
CTCGGTGCGT GCACCCTGGG GGTCCCCCGC CTCGGCTCTG TGAGCGAGAT GCCTCTGGGA 
CACATCATGA GGCTGGATCT GGAGAAGATC GCCCTGGAGT ACATCGTGCC CTGTCTGCAC 
GAGGTCGGTT TCTGCTACCT GGACAACTTC CTAGGCGAGG TGGTGGGCGA CTGCGTACTG 
GAGCGAGTCA AGCAGCTGCA TTACAACGGG GCCCTGCGTG ACGGCCAGCT GGCGGGGCCC 
CGCGCCGGCG TCTCCAAGCG GCACCTGCGA GGCGACCAGA TCACGTGGAT CGGGGGCAAC 
GAGGAGGGCT GCGAGGCCAT CAACTTCCTC CTGTCCCTCA TCGACAGGCT GGTCCTGTAC 
TGCGGGAGCC GCCTGGGCAA ATACTATGTC AAGGAGCGGT CCAAGGCGAT GGTGGCTTGC 
TATCCAGGAA ATGGAACAGG TTATGTTCGT CATGTGGACA ACCCCAATGG TGATGGCCGC 
TGTATCACCT GTATCTACTA CCTGAATAAG AATTGGGACG CCAAGTTACA TGGAGGGGTC 
CTGCGGATAT TTCCAGAAGG AAAATCATTT GTAGCAGACG TGGAGCCCAT TTTTGATAGA 
CTCCTGTTCT CCTGGTCAGA CCGCAGGAAT CCACATGAAG TTCAGCCCTC CTATGCCACC 
AGGTACGCCA TGACTGTCTG GTACTTCGAT GCTGAAGAAA GGGCAGAAGC CAAAAAGAAA 
TTCAGGAATC TAACTAGAAA AACTGAATCT GCTCTTGCTA AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Changes in Hypoxia-Inducible Factor-1 (HIF-1) and Regulatory Prolyl Hydroxylase (PHD) Enzymes Following Hypoxic-Ischemic Injury in the Neonatal Rat.
Neurochemical research41(3)515-22(2016 Mar)
Chu HX,Jones NM

Depletion of PHD3 protects heart from ischemia/reperfusion injury by inhibiting cardiomyocyte apoptosis.
Journal of molecular and cellular cardiology80156-65(2015 Mar)
Xie L,Pi X,Wang Z,He J,Willis MS,Patterson C

Advanced glycated end-products affect HIF-transcriptional activity in renal cells.
Molecular endocrinology (Baltimore, Md.)27(11)1918-33(2013 Nov)
Bondeva T,Heinzig J,Ruhe C,Wolf G

Prospective study assessing hypoxia-related proteins as markers for the outcome of treatment with sunitinib in advanced clear-cell renal cell carcinoma.
Annals of oncology : official journal of the European Society for Medical Oncology24(9)2409-14(2013 Sep)
Garcia-Donas J,Leandro-Garc?a LJ,Gonz?lez Del Alba A,Morente M,Alemany I,Esteban E,Arranz JA,Climent MA,Gallardo E,Castellano DE,Bellmunt J,Mellado B,Puente J,Moreno F,Font A,Hernando S,Robledo M,Rodr?guez-Antona C

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Blood120(16)3336-44(2012 Oct)
Laitala A,Aro E,Walkinshaw G,M?ki JM,Rossi M,Heikkil? M,Savolainen ER,Arend M,Kivirikko KI,Koivunen P,Myllyharju J

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Egln3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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