Eif2s1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif2s1
Entrez Gene ID	54318
Full Name	eukaryotic translation initiation factor 2 subunit alpha
General protein information	Preferred Names eukaryotic translation initiation factor 2 subunit alpha Names eukaryotic translation initiation factor 2 subunit 1 EIF-2A EIF-2alpha eIF-2-alpha eukaryotic translation initiation factor 2 subunit 1 alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	subunit of translational initiation factor eIF-2; forms ternary complex with methionyl-tRNAi and GTP and promotes the binding of the initiator tRNA to 40 S ribosomal subunits [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019356.1	NP_062229.1	eukaryotic translation initiation factor 2 subunit 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	EIF2S1	NP_787007.1
Danio rerio (zebrafish)	eif2s1b	NP_571875.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011190	XP_309455.4
Saccharomyces cerevisiae (baker's yeast)	SUI2	NP_012540.3
Magnaporthe oryzae (rice blast fungus)	MGG_13200	XP_003712017.1
Arabidopsis thaliana (thale cress)	EIF2 ALPHA	NP_196166.1
Canis lupus familiaris (dog)	EIF2S1	XP_005623592.1
Caenorhabditis elegans (roundworm)	Y37E3.10	NP_490930.1
Neurospora crassa	NCU08277	XP_961905.1
Mus musculus (house mouse)	Eif2s1	NP_080390.1
Rattus norvegicus (Norway rat)	Eif2s1	NP_062229.1
Drosophila melanogaster (fruit fly)	eIF-2alpha	NP_573130.1
Schizosaccharomyces pombe (fission yeast)	tif211	NP_594081.1
Arabidopsis thaliana (thale cress)	AT2G40290	NP_565927.1
Xenopus tropicalis (tropical clawed frog)	eif2s1	NP_001005630.1
Homo sapiens (human)	EIF2S1	NP_004085.1
	EIF2S1	XP_001105924.1
Gallus gallus (chicken)	EIF2S1	NP_001006477.1
Kluyveromyces lactis	KLLA0A11814g	XP_451514.1
Eremothecium gossypii	AGOS_AGR320W	NP_986986.2
Oryza sativa (rice)	Os07g0692800	NP_001060724.1
Pan troglodytes (chimpanzee)	EIF2S1	XP_510016.1
Danio rerio (zebrafish)	eif2s1a	NP_955863.1
Oryza sativa (rice)	Os03g0296400	NP_001049829.1

Gene Ontology
Bibliography

Related articles in PubMed

Exploring the Interaction Between eIF2? Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain.
Beauvais G, Rodriguez-Losada N, Ying L, Zakirova Z, Watson JL, Readhead B, Gadue P, French DL, Ehrlich ME, Gonzalez-Alegre P
Neuroscience371455-468(2017 Dec)

AMP Kinase Activation Alters Oxidant-Induced Stress Granule Assembly by Modulating Cell Signaling and Microtubule Organization.
Mahboubi H, Koromilas AE, Stochaj U
Molecular pharmacology90(4)460-8(2016 Oct)

Amphetamine elevates phosphorylation of eukaryotic initiation factor 2? (eIF2?) in the rat forebrain via activating dopamine D1 and D2 receptors.
Xue B, Fitzgerald CA, Jin DZ, Mao LM, Wang JQ
Brain research1646459-466(2016 09)

A Critical Role of the mTOR/eIF2? Pathway in Hypoxia-Induced Pulmonary Hypertension.
Wang AP, Li XH, Yang YM, Li WQ, Zhang W, Hu CP, Zhang Z, Li YJ
PloS one10(6)e0130806(2015)

eIF2? dephosphorylation in basolateral amygdala mediates reconsolidation of drug memory.
Jian M, Luo YX, Xue YX, Han Y, Shi HS, Liu JF, Yan W, Wu P, Meng SQ, Deng JH, Shen HW, Shi J, Lu L
The Journal of neuroscience : the official journal of the Society for Neuroscience34(30)10010-21(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results demonstrate the sensitivity of eukaryotic initiation factor 2alpha-subunit (eIF2alpha) to stimulant exposure. Amphetamine possesses the ability to stimulate eIF2alpha phosphorylation in striatal and medial prefrontal cortex neurons in vivo in a dopamine D1 and D2 receptor-dependent manner.
Title: Amphetamine elevates phosphorylation of eukaryotic initiation factor 2? (eIF2?) in the rat forebrain via activating dopamine D1 and D2 receptors.

These results suggest a critical role of the mTOR/eIF2alpha pathway in hypoxic vascular remodeling and pulmonary arterial vascular smooth muscle cell proliferation of pulmonary hypertension.
Title: A Critical Role of the mTOR/eIF2? Pathway in Hypoxia-Induced Pulmonary Hypertension.

Dephosphorylation of eIF2alpha in the basolateral amygdala mediates memory reconsolidation of drug-paired stimuli.
Title: eIF2? dephosphorylation in basolateral amygdala mediates reconsolidation of drug memory.

Data suggest maternal overweight due to high-fat diet up-regulates mTOR complex 1 (mechanistic target of rapamycin) activity and down-regulates eIF2 alpha subunit phosphorylation in placenta; such alterations contribute to placental/fetal overgrowth.
Title: Maternal overweight induced by a diet with high content of saturated fat activates placental mTOR and eIF2alpha signaling and increases fetal growth in rats.

eIF2a is hyperphosphorylated in the cortex of aged rats.
Title: ApoE ?4 is associated with eIF2? phosphorylation and impaired learning in young mice.

The following Eif2s1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2s1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04534	NM_019356.1 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 2 subunit alpha (Eif2s1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif2s1 ( NM_019356.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062229.1 Rattus norvegicus eukaryotic translation initiation factor 2 subunit alpha (Eif2s1), mRNA.

Price & Availability↑

CloneID	ORa04534
Clone ID Related Accession (Same CDS sequence)	NM_019356.1
Accession Version	NM_019356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic translation initiation factor 2 subunit 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02646.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02646.1, BC087019.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGGTC TAAGTTGTAG ATTTTATCAA CACAAATTTC CTGAGGTCGA AGATGTAGTG 
ATGGTGAATG TAAGATCCAT TGCTGAAATG GGGGCCTATG TCAGCTTGTT GGAATATAAT 
AACATTGAAG GCATGATTCT TCTTAGTGAA TTATCCAGAC GACGTATCCG TTCTATAAAC 
AAACTGATCC GAATTGGCAG AAATGAATGT GTAGTTGTCA TTAGAGTGGA TAAAGAAAAA 
GGATATATAG ATTTGTCAAA AAGAAGAGTT TCTCCAGAGG AAGCAATCAA ATGTGAAGAC 
AAATTCACAA AATCCAAAAC TGTTTATAGC ATTCTTCGCC ATGTTGCTGA GGTATTAGAG 
TATACCAAGG ATGAGCAGCT GGAAAGCCTA TTCCAGAGGA CTGCCTGGGT CTTTGATGAC 
AAGTACAAGA GACCTGGATA TGGTGCCTAT GATGCCTTTA AGCATGCAGT CTCAGACCCA 
TCTATCTTGG ATAGTTTAGA TTTGAATGAA GATGAAAGAG AAGTACTCAT TAACAATATC 
AATAGGCGTT TGACCCCACA AGCTGTCAAG ATTCGAGCAG ATATTGAGGT AGCTTGCTAT 
GGTTACGAAG GCATTGATGC TGTAAAAGAA GCCCTGAGAG CAGGTTTGAA TTGTTCTACA 
GAAACCATGC CCATCAAGAT TAATCTAATA GCTCCACCCA GGTATGTGAT GACAACAACG 
ACCCTAGAGA GGACAGAAGG ACTCTCTGTT CTCAATCAGG CTATGGCAGT CATCAAAGAA 
AAGATTGAGG AGAAGAGGGG AGTGTTCAAT GTTCAGATGG AGCCCAAAGT GGTTACAGAT 
ACAGATGAGA CTGAACTTGC AAGGCAGCTG GAACGGCTTG AGAGAGAAAA TGCAGAAGTG 
GATGGAGATG ATGATGCAGA AGAAATGGAA GCCAAAGCTG AAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062229.1
CDS	28..975
Translation	MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLERENAEVDGDDDAEEMEAKAED

Target ORF information:

RefSeq Version	NM_019356.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 2 subunit alpha (Eif2s1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019356.1

ORF Insert Sequence:

ATGCCGGGTC TAAGTTGTAG ATTTTATCAA CACAAATTTC CTGAGGTCGA AGATGTAGTG 
ATGGTGAATG TAAGATCCAT TGCTGAAATG GGGGCCTATG TCAGCTTGTT GGAATATAAT 
AACATTGAAG GCATGATTCT TCTTAGTGAA TTATCCAGAC GACGTATCCG TTCTATAAAC 
AAACTGATCC GAATTGGCAG AAATGAATGT GTAGTTGTCA TTAGAGTGGA TAAAGAAAAA 
GGATATATAG ATTTGTCAAA AAGAAGAGTT TCTCCAGAGG AAGCAATCAA ATGTGAAGAC 
AAATTCACAA AATCCAAAAC TGTTTATAGC ATTCTTCGCC ATGTTGCTGA GGTATTAGAG 
TATACCAAGG ATGAGCAGCT GGAAAGCCTA TTCCAGAGGA CTGCCTGGGT CTTTGATGAC 
AAGTACAAGA GACCTGGATA TGGTGCCTAT GATGCCTTTA AGCATGCAGT CTCAGACCCA 
TCTATCTTGG ATAGTTTAGA TTTGAATGAA GATGAAAGAG AAGTACTCAT TAACAATATC 
AATAGGCGTT TGACCCCACA AGCTGTCAAG ATTCGAGCAG ATATTGAGGT AGCTTGCTAT 
GGTTACGAAG GCATTGATGC TGTAAAAGAA GCCCTGAGAG CAGGTTTGAA TTGTTCTACA 
GAAACCATGC CCATCAAGAT TAATCTAATA GCTCCACCCA GGTATGTGAT GACAACAACG 
ACCCTAGAGA GGACAGAAGG ACTCTCTGTT CTCAATCAGG CTATGGCAGT CATCAAAGAA 
AAGATTGAGG AGAAGAGGGG AGTGTTCAAT GTTCAGATGG AGCCCAAAGT GGTTACAGAT 
ACAGATGAGA CTGAACTTGC AAGGCAGCTG GAACGGCTTG AGAGAGAAAA TGCAGAAGTG 
GATGGAGATG ATGATGCAGA AGAAATGGAA GCCAAAGCTG AAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exploring the Interaction Between eIF2? Dysregulation, Acute Endoplasmic Reticulum Stress and DYT1 Dystonia in the Mammalian Brain.
Neuroscience371455-468(2017 Dec)
Beauvais G,Rodriguez-Losada N,Ying L,Zakirova Z,Watson JL,Readhead B,Gadue P,French DL,Ehrlich ME,Gonzalez-Alegre P

AMP Kinase Activation Alters Oxidant-Induced Stress Granule Assembly by Modulating Cell Signaling and Microtubule Organization.
Molecular pharmacology90(4)460-8(2016 Oct)
Mahboubi H,Koromilas AE,Stochaj U

Amphetamine elevates phosphorylation of eukaryotic initiation factor 2? (eIF2?) in the rat forebrain via activating dopamine D1 and D2 receptors.
Brain research1646459-466(2016 09)
Xue B,Fitzgerald CA,Jin DZ,Mao LM,Wang JQ

A Critical Role of the mTOR/eIF2? Pathway in Hypoxia-Induced Pulmonary Hypertension.
PloS one10(6)e0130806(2015)
Wang AP,Li XH,Yang YM,Li WQ,Zhang W,Hu CP,Zhang Z,Li YJ

eIF2? dephosphorylation in basolateral amygdala mediates reconsolidation of drug memory.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(30)10010-21(2014 Jul)
Jian M,Luo YX,Xue YX,Han Y,Shi HS,Liu JF,Yan W,Wu P,Meng SQ,Deng JH,Shen HW,Shi J,Lu L

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Eif2s1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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