Itpkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itpkb
Entrez Gene ID	54260
Full Name	inositol-trisphosphate 3-kinase B
Synonyms	IP3K B
General protein information	Preferred Names inositol-trisphosphate 3-kinase B Names inositol-trisphosphate 3-kinase B IP3 3-kinase B IP3K-B inositol 1,4,5-trisphosphate 3-kinase B insP 3-kinase B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q26
Summary	regulates inositol phosphate metabolism which is required in cell signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019312.2	NP_062185.2	inositol-trisphosphate 3-kinase B
XM_017598917.1	XP_017454406.1	inositol-trisphosphate 3-kinase B isoform X1
XM_017598918.1	XP_017454407.1	inositol-trisphosphate 3-kinase B isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	itpkb	XP_002938890.1
Homo sapiens (human)	ITPKB	NP_002212.3
	ITPKB	XP_001089824.2
Mus musculus (house mouse)	Itpkb	NP_001074644.1
Drosophila melanogaster (fruit fly)	IP3K2	NP_001096969.1
Canis lupus familiaris (dog)	ITPKB	XP_547504.2
Bos taurus (cattle)	ITPKB	XP_002707781.2
Danio rerio (zebrafish)	itpkb	XP_691475.2
Pan troglodytes (chimpanzee)	ITPKB	XP_001141610.1
Rattus norvegicus (Norway rat)	Itpkb	NP_062185.2
Gallus gallus (chicken)	ITPKB	XP_415039.2

Gene Ontology
Bibliography

Related articles in PubMed

Inositol trisphosphate 3-kinase B (InsP3KB) as a physiological modulator of myelopoiesis.
Jia Y, Loison F, Hattori H, Li Y, Erneux C, Park SY, Gao C, Chai L, Silberstein LE, Schurmans S, Luo HR
Proceedings of the National Academy of Sciences of the United States of America105(12)4739-44(2008 Mar)

Regulation of the localization and activity of inositol 1,4,5-trisphosphate 3-kinase B in intact cells by proteolysis.
Yu JC, Lloyd-Burton SM, Irvine RF, Schell MJ
The Biochemical journal392(Pt 3)435-41(2005 Dec)

Identification and subcellular distribution of endogenous Ins(1,4,5)P(3) 3-kinase B in mouse tissues.
Hascakova-Bartova R, Pouillon V, Dewaste V, Moreau C, Jacques C, Banting G, Schurmans S, Erneux C
Biochemical and biophysical research communications323(3)920-5(2004 Oct)

Inositol (1,4,5) trisphosphate 3 kinase B controls positive selection of T cells and modulates Erk activity.
Wen BG, Pletcher MT, Warashina M, Choe SH, Ziaee N, Wiltshire T, Sauer K, Cooke MP
Proceedings of the National Academy of Sciences of the United States of America101(15)5604-9(2004 Apr)

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ
Genome research14(4)750-7(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The localization and activity of inositol 1,4,5-trisphosphate 3-kinase B(IP3KB) and its modulation by proteolysis are reported.
Title: Regulation of the localization and activity of inositol 1,4,5-trisphosphate 3-kinase B in intact cells by proteolysis.

The following Itpkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itpkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04378	NM_019312.2 Latest version!	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
ORa04378	XM_017598917.1 Latest version!	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00
ORa04378	XM_017598918.1 Latest version!	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$552.30 $789.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itpkb ( NM_019312.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062185.2 Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), mRNA.

Price & Availability↑

CloneID	ORa04378
Clone ID Related Accession (Same CDS sequence)	NM_019312.2 , XM_017598917.1 , XM_017598918.1
Accession Version	NM_019312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	inositol-trisphosphate 3-kinase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA859368.1, BF418570.1, AJ242781.1 and BE113255.1. On Jan 10, 2008 this sequence version replaced NM_019312.1. ##Evidence-Data-START## CDS exon combination :: AJ242781.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062185.2
CDS	495..3299
Translation	MAVYCYALNSLVIMNSTNELKSGSPLPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSAGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVATRILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQTSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDANSRRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGSPASPRCGSPTPMEIDKRTAPSLEHFGTSLTLATKVAASAASAGPHPGHDSVLMEADCELGAMRPWEAHLERRGQFLGRETGSAPEPIRTHIREPPGRVERVHSVGGQGSWTPEVIKRPEEGTVDAQSSELSENPRWSRLPGDPGSVGPEKGGSRIPGIRGPQQTLDSMREGSSALGLLGGSQAAQPGSMDVETGISCGRMLEPLPPGEVTTNLKEPQCLPGDRMGMQPESSIVWPSAVEEAPLIWTCDTGIQLKGTWRSQDGDAHPSCQEKSPDQKDKACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRETLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLNNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	NM_019312.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019312.2

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_017598917.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454406.1 Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa04378
Clone ID Related Accession (Same CDS sequence)	NM_019312.2 , XM_017598917.1 , XM_017598918.1
Accession Version	XM_017598917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inositol-trisphosphate 3-kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454406.1
CDS	1084..3888
Translation	MAVYCYALNSLVIMNSTNELKSGSPLPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSAGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVATRILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQTSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDANSRRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGSPASPRCGSPTPMEIDKRTAPSLEHFGTSLTLATKVAASAASAGPHPGHDSVLMEADCELGAMRPWEAHLERRGQFLGRETGSAPEPIRTHIREPPGRVERVHSVGGQGSWTPEVIKRPEEGTVDAQSSELSENPRWSRLPGDPGSVGPEKGGSRIPGIRGPQQTLDSMREGSSALGLLGGSQAAQPGSMDVETGISCGRMLEPLPPGEVTTNLKEPQCLPGDRMGMQPESSIVWPSAVEEAPLIWTCDTGIQLKGTWRSQDGDAHPSCQEKSPDQKDKACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRETLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLNNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	XM_017598917.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598917.1

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itpkb ( XM_017598918.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454407.1 Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

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CloneID	ORa04378
Clone ID Related Accession (Same CDS sequence)	NM_019312.2 , XM_017598917.1 , XM_017598918.1
Accession Version	XM_017598918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inositol-trisphosphate 3-kinase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454407.1
CDS	1051..3855
Translation	MAVYCYALNSLVIMNSTNELKSGSPLPSGSETPQPSGRAALSPGSVFSPGRGASFLFPPAESLSLEEPGSAGGWRSGRRRLNSSSGSGGGSSSSNSSSSSGVGSPSWAGRLRGDAQQVVATRILSPPGPEEAQRKLRILQRELQNVQVNQKVGMFEAQIQAQTSAIQAPRSPRLGRARSPSPCPFRSSSQPPERVLAPCSPSEERRTKSWGEQCTETPDANSRRRSRLSTHPSKDKEGVAPLLGPASPTRLGTQSPSTSVRMERGSPASPRCGSPTPMEIDKRTAPSLEHFGTSLTLATKVAASAASAGPHPGHDSVLMEADCELGAMRPWEAHLERRGQFLGRETGSAPEPIRTHIREPPGRVERVHSVGGQGSWTPEVIKRPEEGTVDAQSSELSENPRWSRLPGDPGSVGPEKGGSRIPGIRGPQQTLDSMREGSSALGLLGGSQAAQPGSMDVETGISCGRMLEPLPPGEVTTNLKEPQCLPGDRMGMQPESSIVWPSAVEEAPLIWTCDTGIQLKGTWRSQDGDAHPSCQEKSPDQKDKACSPSNIPAIPAVIITDMGAQEDGGLEEIQGSPRGPLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPNSAFLHTLDQQKPRVSKSWRKIKNMVQWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCLDRLMADVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMYQKMVEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGSVNRDFKKTKTREQVTEAFREFTKGNQNILIAYRDRLKAIRETLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTLQHDVPWQEGNREDGYLSGLNNLIDILTEMSQGSPLT

Target ORF information:

RefSeq Version	XM_017598918.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus inositol-trisphosphate 3-kinase B (Itpkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598918.1

ORF Insert Sequence:

ATGGCTGTGT ACTGCTATGC CCTCAATAGC CTGGTGATCA TGAACAGCAC CAACGAGCTG 
AAGAGTGGCA GCCCCCTGCC CAGCGGCAGC GAAACGCCTC AGCCCTCCGG GAGGGCCGCG 
CTGAGCCCCG GCAGCGTCTT CAGCCCTGGG AGAGGCGCCT CCTTTCTCTT CCCCCCAGCA 
GAGTCGCTGT CGCTGGAGGA GCCTGGGAGT GCTGGGGGTT GGCGCAGCGG CCGACGGAGG 
CTGAATAGTA GCAGCGGTAG CGGAGGTGGC AGCAGCAGCA GCAACAGCAG CAGCAGCAGT 
GGCGTGGGCA GTCCCAGTTG GGCTGGCCGC CTGCGAGGGG ACGCGCAGCA GGTGGTGGCG 
ACCCGCATCC TCTCCCCACC TGGGCCGGAG GAGGCCCAGA GGAAGCTGAG GATTCTGCAG 
CGCGAATTGC AAAATGTGCA GGTGAACCAG AAAGTGGGCA TGTTCGAGGC GCAAATCCAG 
GCACAGACCT CTGCTATTCA AGCGCCCCGA AGCCCGCGTT TGGGTAGGGC TCGTTCGCCC 
TCCCCGTGTC CCTTCCGAAG CAGCAGCCAG CCTCCTGAAA GGGTCTTGGC TCCATGTTCC 
CCAAGTGAGG AACGGAGAAC AAAGTCCTGG GGAGAACAAT GTACAGAGAC CCCAGATGCC 
AACTCCAGGA GGAGAAGCAG ACTCAGCACA CACCCCTCGA AGGACAAGGA GGGAGTGGCC 
CCTCTTTTAG GCCCAGCCAG CCCGACCAGG TTAGGGACTC AGAGTCCATC TACTTCAGTG 
AGAATGGAAA GAGGTTCCCC GGCCAGTCCC CGCTGTGGCT CACCCACACC CATGGAAATT 
GACAAGAGGA CTGCTCCCTC ACTGGAGCAC TTTGGGACTA GCTTAACGTT GGCCACTAAA 
GTGGCAGCTT CGGCCGCATC CGCTGGACCA CACCCTGGAC ATGATTCTGT TCTCATGGAG 
GCAGACTGTG AGCTAGGGGC CATGCGCCCC TGGGAGGCCC ACCTGGAGAG ACGGGGGCAG 
TTTCTGGGCA GGGAGACCGG CTCAGCCCCA GAGCCTATCC GGACCCACAT TAGAGAACCC 
CCTGGAAGGG TGGAAAGAGT TCATTCTGTT GGTGGCCAGG GCTCCTGGAC ACCTGAAGTC 
ATCAAAAGAC CAGAAGAGGG AACTGTGGAT GCCCAAAGCT CAGAGCTCTC AGAGAACCCG 
AGATGGTCTA GACTGCCTGG AGACCCGGGT TCCGTAGGGC CTGAGAAGGG AGGTAGTAGG 
ATCCCAGGAA TCCGAGGACC CCAGCAGACC CTGGACAGCA TGAGAGAAGG GTCTTCAGCA 
CTGGGCTTGC TTGGGGGCAG CCAGGCAGCA CAGCCAGGGA GCATGGATGT GGAGACAGGC 
ATTAGTTGTG GCAGAATGCT GGAACCCTTA CCACCTGGGG AAGTAACAAC AAATTTGAAA 
GAACCCCAGT GCCTCCCTGG GGACAGGATG GGGATGCAGC CTGAGAGTTC CATAGTTTGG 
CCCAGTGCTG TGGAGGAAGC TCCCCTGATC TGGACGTGTG ACACAGGGAT ACAGTTAAAG 
GGGACTTGGA GAAGCCAAGA TGGAGATGCT CATCCTAGCT GCCAAGAGAA GTCCCCAGAC 
CAGAAGGACA AGGCCTGCAG CCCCAGCAAC ATCCCGGCCA TCCCTGCAGT CATCATTACA 
GATATGGGTG CTCAGGAGGA TGGAGGGTTA GAGGAGATCC AAGGAAGCCC TCGGGGTCCC 
CTGCCTCTGA GGAAGCTGTC GTCCTCCTCA GCCTCCTCCA CTGGCTTCTC CTCTTCCTAT 
GAGGACTCGG AGGAGGACAT CTCCAGTGAC CCTGAGCGCA CTCTGGACCC CAACTCAGCC 
TTTTTGCATA CCTTGGACCA GCAGAAGCCC AGAGTGAGCA AGTCATGGAG GAAGATAAAG 
AACATGGTGC AGTGGTCCCC CTTTGTCATG TCCTTCAAGA AGAAGTACCC CTGGATCCAG 
CTGGCAGGAC ATGCAGGGAG CTTCAAGGCA GCTGCGAATG GCCGTATCCT TAAGAAGCAT 
TGTGAGTCTG AACAGCGATG CCTGGACCGG TTAATGGCGG ATGTGCTGAG GCCCTTCGTG 
CCAGCCTACC ATGGCGACGT GGTGAAGGAC GGGGAACGCT ACAACCAGAT GGACGACCTG 
CTGGCTGACT TCGATTCACC CTGCGTGATG GACTGCAAGA TGGGCATCAG GACATACCTG 
GAGGAAGAAC TCACCAAGGC CCGGAAGAAG CCTAGCTTGC GGAAGGACAT GTACCAGAAG 
ATGGTGGAGG TGGACCCTGA GGCCCCCACT GAAGAGGAGA AAGCCCAGAG AGCTGTGACC 
AAGCCACGTT ATATGCAGTG GCGGGAAACC ATCAGTTCCA CAGCTACCTT AGGCTTCAGG 
ATCGAGGGCA TCAAGAAGGA AGATGGCTCT GTGAACCGTG ACTTCAAGAA GACCAAAACA 
AGGGAGCAGG TCACTGAGGC CTTCAGAGAA TTCACTAAAG GAAACCAGAA CATCCTGATT 
GCCTACCGGG ACCGGTTGAA GGCCATTCGA GAAACCCTGG AAGTCTCTCC CTTCTTCAAG 
TGCCATGAGG TCATTGGCAG CTCTCTCCTC TTCATCCACG ACAAGAAGGA GCAAGCCAAG 
GTGTGGATGA TTGACTTTGG GAAAACCACG CCCCTTCCGG AAGGCCAGAC CCTACAACAC 
GATGTCCCCT GGCAGGAGGG GAACCGGGAG GATGGCTACC TCTCAGGGCT GAACAACCTC 
ATCGACATCC TGACAGAAAT GTCCCAGGGC AGCCCACTCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inositol trisphosphate 3-kinase B (InsP3KB) as a physiological modulator of myelopoiesis.
Proceedings of the National Academy of Sciences of the United States of America105(12)4739-44(2008 Mar)
Jia Y,Loison F,Hattori H,Li Y,Erneux C,Park SY,Gao C,Chai L,Silberstein LE,Schurmans S,Luo HR

Regulation of the localization and activity of inositol 1,4,5-trisphosphate 3-kinase B in intact cells by proteolysis.
The Biochemical journal392(Pt 3)435-41(2005 Dec)
Yu JC,Lloyd-Burton SM,Irvine RF,Schell MJ

Identification and subcellular distribution of endogenous Ins(1,4,5)P(3) 3-kinase B in mouse tissues.
Biochemical and biophysical research communications323(3)920-5(2004 Oct)
Hascakova-Bartova R,Pouillon V,Dewaste V,Moreau C,Jacques C,Banting G,Schurmans S,Erneux C

Inositol (1,4,5) trisphosphate 3 kinase B controls positive selection of T cells and modulates Erk activity.
Proceedings of the National Academy of Sciences of the United States of America101(15)5604-9(2004 Apr)
Wen BG,Pletcher MT,Warashina M,Choe SH,Ziaee N,Wiltshire T,Sauer K,Cooke MP

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Genome research14(4)750-7(2004 Apr)
Kwitek AE,Gullings-Handley J,Yu J,Carlos DC,Orlebeke K,Nie J,Eckert J,Lemke A,Andrae JW,Bromberg S,Pasko D,Chen D,Scheetz TE,Casavant TL,Soares MB,Sheffield VC,Tonellato PJ,Jacob HJ

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Itpkb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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