Slc6a12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc6a12
Entrez Gene ID	50676
Full Name	solute carrier family 6 member 12
Synonyms	BGT1,Gat1,RNU28927,VGAT
General protein information	Preferred Names solute carrier family 6 member 12 Names sodium- and chloride-dependent betaine transporter Betaine/GABA transporter 1 GABA transporter 1 Na(+)/Cl(-) betaine/GABA transporter solute carrier family 6 (neurotransmitter transporter), member 12 solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 vesicular GABA transporter
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42
Summary	sodium/chloride-dependent neurotransmitter transport protein; expressed in exocytotic vesicles in GABAergic cells; associated with exocysts [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017335.1	NP_059031.1	sodium- and chloride-dependent betaine transporter

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC6A12	NP_001003322.1
Pan troglodytes (chimpanzee)	SLC6A12	XP_003313471.1
Mus musculus (house mouse)	Slc6a12	NP_598422.2
Rattus norvegicus (Norway rat)	Slc6a12	NP_059031.1
	SLC6A12	XP_001093673.1
Homo sapiens (human)	SLC6A12	NP_001116320.1
Gallus gallus (chicken)	SLC6A13	XP_004937977.1
Xenopus tropicalis (tropical clawed frog)	LOC100492029	XP_002941944.2

Gene Ontology
Bibliography

Related articles in PubMed

Sorting of the vesicular GABA transporter to functional vesicle pools by an atypical dileucine-like motif.
Santos MS, Park CK, Foss SM, Li H, Voglmaier SM
The Journal of neuroscience : the official journal of the Society for Neuroscience33(26)10634-46(2013 Jun)

A functional role for both -aminobutyric acid (GABA) transporter-1 and GABA transporter-3 in the modulation of extracellular GABA and GABAergic tonic conductances in the rat hippocampus.
Kersant? F, Rowley SC, Pavlov I, Guti?rrez-Mecinas M, Semyanov A, Reul JM, Walker MC, Linthorst AC
The Journal of physiology591(10)2429-41(2013 May)

Astrocytic GABA transporter GAT-1 dysfunction in experimental absence seizures.
Pirttimaki T, Parri HR, Crunelli V
The Journal of physiology591(4)823-33(2013 Feb)

GABA transporter subtype 1 and GABA transporter subtype 3 modulate glutamatergic transmission via activation of presynaptic GABA(B) receptors in the rat globus pallidus.
Jin XT, Par? JF, Smith Y
The European journal of neuroscience36(4)2482-92(2012 Aug)

Brain-derived neurotrophic factor (BDNF) enhances GABA transport by modulating the trafficking of GABA transporter-1 (GAT-1) from the plasma membrane of rat cortical astrocytes.
Vaz SH, J?rgensen TN, Crist?v?o-Ferreira S, Duflot S, Ribeiro JA, Gether U, Sebasti?o AM
The Journal of biological chemistry286(47)40464-76(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we report the identification of a unique amino acid motif in the vesicular GABA transporter (VGAT) that controls its synaptic localization and activity-dependent recycling
Title: Sorting of the vesicular GABA transporter to functional vesicle pools by an atypical dileucine-like motif.

GAT-1, which is predominantly presynaptically located, is the major GABA transporter in the hippocampus under baseline, quiescent conditions.
Title: A functional role for both -aminobutyric acid (GABA) transporter-1 and GABA transporter-3 in the modulation of extracellular GABA and GABAergic tonic conductances in the rat hippocampus.

The results demonstrate a malfunction of GAT-1 in thalamic astrocytes of a well-established genetic model of absence epilepsy, and indicate that this abnormality is not a reflection of a general pathology affecting all astrocytic functions.
Title: Astrocytic GABA transporter GAT-1 dysfunction in experimental absence seizures.

This study provides evidence that the GAT-1-mediated regulation of extracellular GABA can modulate glutamatergic transmission at the subthalamopallidal synapse in the rat globus pallidus.
Title: GABA transporter subtype 1 and GABA transporter subtype 3 modulate glutamatergic transmission via activation of presynaptic GABA(B) receptors in the rat globus pallidus.

BDNF enhances the time span of GAT-1 molecules at the plasma membrane of astrocytes.
Title: Brain-derived neurotrophic factor (BDNF) enhances GABA transport by modulating the trafficking of GABA transporter-1 (GAT-1) from the plasma membrane of rat cortical astrocytes.

The following Slc6a12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc6a12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00049	NM_017335.1 Latest version!	Rattus norvegicus solute carrier family 6 member 12 (Slc6a12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc6a12 ( NM_017335.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059031.1 Rattus norvegicus solute carrier family 6 member 12 (Slc6a12), mRNA.

Price & Availability↑

CloneID	ORa00049
Clone ID Related Accession (Same CDS sequence)	NM_017335.1
Accession Version	NM_017335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	sodium- and chloride-dependent betaine transporter
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U28927.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U28927.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCATCGG GAGCTTGGGA AGAAAGGGTC TCCCTCTCCA GTATGGACAG AAAGGTTACA 
GTCCATGAAG ATGGGTGTCC TGTGGTCTCT TGGGTCCCTG AGGAGGGAGA GATGATGGAC 
CAGAAAGACA AGGACCAGGT GAAGGATCGA GGCCAATGGA CCAACAAGAT GGAGTTCGTA 
CTGTCAGTGG CGGGAGAGAT CATTGGCTTA GGCAACGTCT GGAGGTTTCC CTATCTCTGC 
TACAAGAACG GGGGAGGAGC CTTCTTCATC CCCTACTTCA TCTTCTTCTT CAGCTGTGGT 
ATCCCAGTGT TCTTCCTGGA GGTGGCACTG GGTCAGTATA GCAGCCAGGG GAGTGTTACT 
GCTTGGAGGA AGATCTGTCC CCTTCTCCAA GGCATTGGCA TGGCATCTGT GGTCATCGAG 
TCTTATTTGA ACATCTACTA CATCATCATC CTTGCCTGGG CTCTCTTCTA CCTGTTCAGC 
TCCTTCACCT GGGAGCTTCC CTGGACAACC TGCACCAACT CCTGGAACAC AGAACATTGC 
GTGGACTTTC TGAACTACTC ATCGACCAGA GCCGCAAGCT ACTCTGAGAA CTTCACCTCA 
CCAGTCATGG AATTCTGGGA GAGACGGGTT TTGGGTATTA CATCAGGCAT CCATGACCTG 
GGGTCCCTGC GCTGGGAGCT GGCCCTGTGC CTCCTGCTCG CCTGGATCAT CTGCTACTTC 
TGCATCTGGA AGGGAGTCAA GTCCACGGGC AAGGTTGTTT ACTTCACAGC CACTTTTCCC 
TACCTGATGC TGATTATTCT TCTGATCCGG GGTGTTACCC TTCCTGGAGC CTACCAGGGC 
ATCGTCTTCT ACCTGAAGCC AGACTTGCTC CGCCTCAAGG ACCCCCAGGT GTGGATGGAT 
GCGGGCACCC AGATCTTCTT TTCCTTTGCC ATCTGCCAGG GGTGCCTCAC GGCCCTGGGC 
AGCTACAACA AGTACCACAA CAACTGCTAC AGGGACTCCA TCGCCCTCTG CTTCCTGAAC 
AGTGCCACCA GCTTCGTGGC TGGTTTTGTT GTCTTCTCCA TCCTGGGCTT CATGGCTCAG 
GAGCAGGGCG TGCCCATTTC TGAAGTGGCT GAGTCAGGTC CTGGACTGGC TTTCATTGCC 
TTCCCCAAAG CAGTGACGAT GATGCCTTTG TCCCAGCTGT GGTCCTGCCT CTTCTTCCTC 
ATGCTCCTAT TTCTAGGGCT GGACAGCCAG TTCGTCTGTA TGGAGTGCCT GGTCACTGCT 
TCCATGGATA TGTTTCCTCA GCAGCTCCGA AAGAGAGGCC GGCGCGAGCT GCTGATCCTC 
GCAGTTGCTA TCGTGTGCTA CTTGATGGGG CTCCTTCTGG TCACTGAGGG TGGGATGTAC 
ATTTTCCAGC TCTTTGACTA CTACGCCTCC AGCGGAATAT GCCTACTCTT CCTCTCCTTG 
TTTGAGGTCA TCTGCATTGG CTGGGTGTAC GGGGCGGATC GTTTCTATGA CAACGTGGAG 
GACATGATTG GCTATCGGCC ATGGCCCTTG GTGAAGATCT CTTGGCTCTT CCTGACCCCT 
GGTCTTTGCC TGGCCACCTT CTTCTTCTCC TTGAGCAAGT ATACACCTCT CAAATACAAC 
AATGTCTACA TATACCCTTC TTGGGGATAC TCCATTGGAT GGCTCTTGGC TTTTTCCTCC 
ATGGCCTGTG TACCGCTCTT TATTATCATC ACCCTCCTGA AGACCCAGGG CTCCTTCAAG 
AAGCGCCTTC AAAGACTCAT CACGCCAGAT CCCAGTCTGC CCCAGCCAGG GAGGCGTTCA 
CCTCAGGATG GCTCCTCTGC TCAAAACTGT TCGACCTCTC CAGTCAAGCA AGAACTCATT 
GCCTGGGAGA AGGAGACGCA TTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059031.1
CDS	305..2191
Translation	MSSGAWEERVSLSSMDRKVTVHEDGCPVVSWVPEEGEMMDQKDKDQVKDRGQWTNKMEFVLSVAGEIIGLGNVWRFPYLCYKNGGGAFFIPYFIFFFSCGIPVFFLEVALGQYSSQGSVTAWRKICPLLQGIGMASVVIESYLNIYYIIILAWALFYLFSSFTWELPWTTCTNSWNTEHCVDFLNYSSTRAASYSENFTSPVMEFWERRVLGITSGIHDLGSLRWELALCLLLAWIICYFCIWKGVKSTGKVVYFTATFPYLMLIILLIRGVTLPGAYQGIVFYLKPDLLRLKDPQVWMDAGTQIFFSFAICQGCLTALGSYNKYHNNCYRDSIALCFLNSATSFVAGFVVFSILGFMAQEQGVPISEVAESGPGLAFIAFPKAVTMMPLSQLWSCLFFLMLLFLGLDSQFVCMECLVTASMDMFPQQLRKRGRRELLILAVAIVCYLMGLLLVTEGGMYIFQLFDYYASSGICLLFLSLFEVICIGWVYGADRFYDNVEDMIGYRPWPLVKISWLFLTPGLCLATFFFSLSKYTPLKYNNVYIYPSWGYSIGWLLAFSSMACVPLFIIITLLKTQGSFKKRLQRLITPDPSLPQPGRRSPQDGSSAQNCSTSPVKQELIAWEKETHL

Target ORF information:

RefSeq Version	NM_017335.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 6 member 12 (Slc6a12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017335.1

ORF Insert Sequence:

ATGTCATCGG GAGCTTGGGA AGAAAGGGTC TCCCTCTCCA GTATGGACAG AAAGGTTACA 
GTCCATGAAG ATGGGTGTCC TGTGGTCTCT TGGGTCCCTG AGGAGGGAGA GATGATGGAC 
CAGAAAGACA AGGACCAGGT GAAGGATCGA GGCCAATGGA CCAACAAGAT GGAGTTCGTA 
CTGTCAGTGG CGGGAGAGAT CATTGGCTTA GGCAACGTCT GGAGGTTTCC CTATCTCTGC 
TACAAGAACG GGGGAGGAGC CTTCTTCATC CCCTACTTCA TCTTCTTCTT CAGCTGTGGT 
ATCCCAGTGT TCTTCCTGGA GGTGGCACTG GGTCAGTATA GCAGCCAGGG GAGTGTTACT 
GCTTGGAGGA AGATCTGTCC CCTTCTCCAA GGCATTGGCA TGGCATCTGT GGTCATCGAG 
TCTTATTTGA ACATCTACTA CATCATCATC CTTGCCTGGG CTCTCTTCTA CCTGTTCAGC 
TCCTTCACCT GGGAGCTTCC CTGGACAACC TGCACCAACT CCTGGAACAC AGAACATTGC 
GTGGACTTTC TGAACTACTC ATCGACCAGA GCCGCAAGCT ACTCTGAGAA CTTCACCTCA 
CCAGTCATGG AATTCTGGGA GAGACGGGTT TTGGGTATTA CATCAGGCAT CCATGACCTG 
GGGTCCCTGC GCTGGGAGCT GGCCCTGTGC CTCCTGCTCG CCTGGATCAT CTGCTACTTC 
TGCATCTGGA AGGGAGTCAA GTCCACGGGC AAGGTTGTTT ACTTCACAGC CACTTTTCCC 
TACCTGATGC TGATTATTCT TCTGATCCGG GGTGTTACCC TTCCTGGAGC CTACCAGGGC 
ATCGTCTTCT ACCTGAAGCC AGACTTGCTC CGCCTCAAGG ACCCCCAGGT GTGGATGGAT 
GCGGGCACCC AGATCTTCTT TTCCTTTGCC ATCTGCCAGG GGTGCCTCAC GGCCCTGGGC 
AGCTACAACA AGTACCACAA CAACTGCTAC AGGGACTCCA TCGCCCTCTG CTTCCTGAAC 
AGTGCCACCA GCTTCGTGGC TGGTTTTGTT GTCTTCTCCA TCCTGGGCTT CATGGCTCAG 
GAGCAGGGCG TGCCCATTTC TGAAGTGGCT GAGTCAGGTC CTGGACTGGC TTTCATTGCC 
TTCCCCAAAG CAGTGACGAT GATGCCTTTG TCCCAGCTGT GGTCCTGCCT CTTCTTCCTC 
ATGCTCCTAT TTCTAGGGCT GGACAGCCAG TTCGTCTGTA TGGAGTGCCT GGTCACTGCT 
TCCATGGATA TGTTTCCTCA GCAGCTCCGA AAGAGAGGCC GGCGCGAGCT GCTGATCCTC 
GCAGTTGCTA TCGTGTGCTA CTTGATGGGG CTCCTTCTGG TCACTGAGGG TGGGATGTAC 
ATTTTCCAGC TCTTTGACTA CTACGCCTCC AGCGGAATAT GCCTACTCTT CCTCTCCTTG 
TTTGAGGTCA TCTGCATTGG CTGGGTGTAC GGGGCGGATC GTTTCTATGA CAACGTGGAG 
GACATGATTG GCTATCGGCC ATGGCCCTTG GTGAAGATCT CTTGGCTCTT CCTGACCCCT 
GGTCTTTGCC TGGCCACCTT CTTCTTCTCC TTGAGCAAGT ATACACCTCT CAAATACAAC 
AATGTCTACA TATACCCTTC TTGGGGATAC TCCATTGGAT GGCTCTTGGC TTTTTCCTCC 
ATGGCCTGTG TACCGCTCTT TATTATCATC ACCCTCCTGA AGACCCAGGG CTCCTTCAAG 
AAGCGCCTTC AAAGACTCAT CACGCCAGAT CCCAGTCTGC CCCAGCCAGG GAGGCGTTCA 
CCTCAGGATG GCTCCTCTGC TCAAAACTGT TCGACCTCTC CAGTCAAGCA AGAACTCATT 
GCCTGGGAGA AGGAGACGCA TTTGTAG