Kcnj3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj3
Entrez Gene ID	50599
Full Name	potassium voltage-gated channel subfamily J member 3
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 3 Names G protein-activated inward rectifier potassium channel 1 GIRK-1 GIRK1 KGA KGB1 inward rectifier K(+) channel Kir3.1 potassium channel inwarding rectifying channel subfamily J member 3 potassium channel subunit Kir3.1 type 3 delta potassium channel, inwardly rectifying subfamily J member 3 potassium inwardly-rectifying channel, subfamily J, member 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q21
Summary	inwardly-rectifying potassium channel; gated by G-protein beta gamma-subunits [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031610.3	NP_113798.1	G protein-activated inward rectifier potassium channel 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KCNJ3	XP_545477.2
Rattus norvegicus (Norway rat)	Kcnj3	NP_113798.1
Mus musculus (house mouse)	Kcnj3	NP_032452.1
Bos taurus (cattle)	KCNJ3	NP_001179553.1
Gallus gallus (chicken)	KCNJ3	NP_990735.1
Danio rerio (zebrafish)	si:ch73-83c10.1	XP_002663356.2
Xenopus tropicalis (tropical clawed frog)	kcnj3	XP_002936698.1
Homo sapiens (human)	KCNJ3	NP_002230.1
	KCNJ3	XP_001084969.1

Gene Ontology
Bibliography

Related articles in PubMed

Prolonged seizure activity causes caspase dependent cleavage and dysfunction of G-protein activated inwardly rectifying potassium channels.
Baculis BC, Weiss AC, Pang W, Jeong HG, Lee JH, Liu DC, Tsai NP, Chung HJ
Scientific reports7(1)12313(2017 Sep)

Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.
Li Y, Fu X, Zhang Z, Yu B
Molecular and cellular biochemistry429(1-2)103-111(2017 May)

Calcium Release from Stores Inhibits GIRK.
Kramer PF, Williams JT
Cell reports17(12)3246-3255(2016 12)

G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy.
Lyu C, Mulder J, Barde S, Sahlholm K, Zeberg H, Nilsson J, ?rhem P, H?kfelt T, Fried K, Shi TJ
Molecular pain1144(2015 Jul)

Pharmacological Characterization of 5-HT1A Autoreceptor-Coupled GIRK Channels in Rat Dorsal Raphe 5-HT Neurons.
Montalbano A, Corradetti R, Mlinar B
PloS one10(10)e0140369(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study provides evidence that Kir3.1 is involved in the pathogenesis of sinus bradycardia since its ihibition with siRNA can reverse the sinus bradycardia.
Title: Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.

Transient activation of mGluRs decreased the GIRK current evoked by GABAB and D2 receptors, although less efficaciously for D2.
Title: Calcium Release from Stores Inhibits GIRK.

Fourteen days after axotomy, GIRK1 and GIRK2 were down-regulated in DRG neurons at the mRNA and protein levels.
Title: G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy.

Findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.
Title: Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex.

inwardly rectifying K+ channels coupled to 5-HT1A autoreceptors display pharmacological properties generally expected for neuronal GIRK channels, but different from GIRK1-GIRK2 heteromers
Title: Pharmacological Characterization of 5-HT1A Autoreceptor-Coupled GIRK Channels in Rat Dorsal Raphe 5-HT Neurons.

The following Kcnj3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12601	NM_031610.3 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 3 (Kcnj3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$293.30 $419.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj3 ( NM_031610.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113798.1 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 3 (Kcnj3), mRNA.

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CloneID	ORa12601
Clone ID Related Accession (Same CDS sequence)	NM_031610.3
Accession Version	NM_031610.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	G protein-activated inward rectifier potassium channel 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L25264.1. On Jun 10, 2007 this sequence version replaced NM_031610.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L25264.1, U09243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTACCAGG TAGTGACCAC TTCGTCCAGC 
GGTTCGGGCT TGCAGCCCCA GGGGCCAGGA CAGGGCCCAC AGCAGCAGCT TGTACCCAAG 
AAGAAACGGC AGCGGTTCGT GGACAAGAAC GGTCGGTGCA ATGTGCAGCA CGGCAACCTG 
GGCAGCGAGA CCAGTCGCTA CCTTTCCGAC CTCTTCACTA CCCTGGTGGA TCTCAAGTGG 
CGTTGGAACC TCTTTATCTT CATCCTCACC TACACCGTGG CCTGGCTCTT CATGGCGTCC 
ATGTGGTGGG TGATCGCTTA TACCCGGGGC GACCTGAACA AAGCCCATGT CGGCAACTAC 
ACTCCCTGTG TGGCCAATGT CTATAACTTC CCCTCTGCCT TCCTTTTCTT CATCGAGACC 
GAGGCCACCA TCGGCTATGG CTACCGCTAC ATCACCGACA AGTGCCCCGA GGGCATCATC 
CTTTTCCTTT TCCAGTCCAT CCTTGGCTCC ATCGTGGACG CTTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAAAAGCGC GCCGAGACCC TCATGTTTAG CGAGCATGCG 
GTTATTTCCA TGAGGGACGG AAAACTCACT CTCATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATCCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTTC TACCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC TCTCACCATT TGCCACGTGA TTGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGGTGGTCGT CATCCTGGAA 
GGCATCGTGG AAACCACAGG GATGACTTGT CAAGCTCGAA CATCATACAC CGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTCCCTGTA ATTTCTTTAG AAGAAGGATT CTTTAAAGTC 
GATTACTCCC AGTTCCATGC AACCTTTGAA GTCCCCACCC CTCCGTACAG TGTGAAAGAG 
CAGGAAGAAA TGCTTCTCAT GTCTTCCCCT TTAATAGCAC CAGCCATAAC CAACAGCAAA 
GAAAGACACA ATTCTGTGGA GTGCTTAGAT GGACTAGATG ACATTAGCAC AAAACTTCCA 
TCGAAGCTGC AGAAAATTAC GGGGAGAGAA GACTTTCCCA AAAAACTCCT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTATAGTTTG GGTGATTTGC CCATGAAACT CCAACGAATA 
AGTTCGGTTC CTGGCAACTC TGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCA 
GATCCCATGA GCCAGTCTGT GGCCGATTTG CCACCGAAGC TTCAAAAGAT GGCTGGAGGA 
CCTACCAGGA TGGAAGGGAA TCTTCCAGCC AAACTAAGAA AAATGAACTC TGACCGCTTC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113798.1
CDS	59..1564
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQGPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEVVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDISTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGPTRMEGNLPAKLRKMNSDRFT

Target ORF information:

RefSeq Version	NM_031610.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 3 (Kcnj3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031610.3

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTACCAGG TAGTGACCAC TTCGTCCAGC 
GGTTCGGGCT TGCAGCCCCA GGGGCCAGGA CAGGGCCCAC AGCAGCAGCT TGTACCCAAG 
AAGAAACGGC AGCGGTTCGT GGACAAGAAC GGTCGGTGCA ATGTGCAGCA CGGCAACCTG 
GGCAGCGAGA CCAGTCGCTA CCTTTCCGAC CTCTTCACTA CCCTGGTGGA TCTCAAGTGG 
CGTTGGAACC TCTTTATCTT CATCCTCACC TACACCGTGG CCTGGCTCTT CATGGCGTCC 
ATGTGGTGGG TGATCGCTTA TACCCGGGGC GACCTGAACA AAGCCCATGT CGGCAACTAC 
ACTCCCTGTG TGGCCAATGT CTATAACTTC CCCTCTGCCT TCCTTTTCTT CATCGAGACC 
GAGGCCACCA TCGGCTATGG CTACCGCTAC ATCACCGACA AGTGCCCCGA GGGCATCATC 
CTTTTCCTTT TCCAGTCCAT CCTTGGCTCC ATCGTGGACG CTTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAAAAGCGC GCCGAGACCC TCATGTTTAG CGAGCATGCG 
GTTATTTCCA TGAGGGACGG AAAACTCACT CTCATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATCCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTTC TACCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC TCTCACCATT TGCCACGTGA TTGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGGTGGTCGT CATCCTGGAA 
GGCATCGTGG AAACCACAGG GATGACTTGT CAAGCTCGAA CATCATACAC CGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTCCCTGTA ATTTCTTTAG AAGAAGGATT CTTTAAAGTC 
GATTACTCCC AGTTCCATGC AACCTTTGAA GTCCCCACCC CTCCGTACAG TGTGAAAGAG 
CAGGAAGAAA TGCTTCTCAT GTCTTCCCCT TTAATAGCAC CAGCCATAAC CAACAGCAAA 
GAAAGACACA ATTCTGTGGA GTGCTTAGAT GGACTAGATG ACATTAGCAC AAAACTTCCA 
TCGAAGCTGC AGAAAATTAC GGGGAGAGAA GACTTTCCCA AAAAACTCCT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTATAGTTTG GGTGATTTGC CCATGAAACT CCAACGAATA 
AGTTCGGTTC CTGGCAACTC TGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCA 
GATCCCATGA GCCAGTCTGT GGCCGATTTG CCACCGAAGC TTCAAAAGAT GGCTGGAGGA 
CCTACCAGGA TGGAAGGGAA TCTTCCAGCC AAACTAAGAA AAATGAACTC TGACCGCTTC 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prolonged seizure activity causes caspase dependent cleavage and dysfunction of G-protein activated inwardly rectifying potassium channels.
Scientific reports7(1)12313(2017 Sep)
Baculis BC,Weiss AC,Pang W,Jeong HG,Lee JH,Liu DC,Tsai NP,Chung HJ

Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.
Molecular and cellular biochemistry429(1-2)103-111(2017 May)
Li Y,Fu X,Zhang Z,Yu B

Calcium Release from Stores Inhibits GIRK.
Cell reports17(12)3246-3255(2016 12)
Kramer PF,Williams JT

G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy.
Molecular pain1144(2015 Jul)
Lyu C,Mulder J,Barde S,Sahlholm K,Zeberg H,Nilsson J,?rhem P,H?kfelt T,Fried K,Shi TJ

Pharmacological Characterization of 5-HT1A Autoreceptor-Coupled GIRK Channels in Rat Dorsal Raphe 5-HT Neurons.
PloS one10(10)e0140369(2015)
Montalbano A,Corradetti R,Mlinar B

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Kcnj3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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