Gja5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gja5
Entrez Gene ID	50563
Full Name	gap junction protein, alpha 5
Synonyms	Cx40
General protein information	Preferred Names gap junction protein, alpha 5 Names gap junction alpha-5 protein connexin 40 gap junction membrane channel protein alpha 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	forms intercellular connoxin channels in gap junctions; mediates cell-cell communication [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019280.1	NP_062153.1	gap junction alpha-5 protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gja5	NP_001258557.1
Pan troglodytes (chimpanzee)	GJA5	XP_513754.2
Bos taurus (cattle)	GJA5	NP_001071490.1
Rattus norvegicus (Norway rat)	Gja5	NP_062153.1
Gallus gallus (chicken)	GJA5	NP_990835.1
Homo sapiens (human)	GJA5	NP_859054.1
Canis lupus familiaris (dog)	GJA5	NP_001017442.2
Xenopus tropicalis (tropical clawed frog)	gja5	XP_004918273.1
	GJA5	XP_001094966.1

Gene Ontology
Bibliography

Related articles in PubMed

Lipopolysaccharide impairs permeability of pulmonary microvascular endothelial cells via Connexin40.
Zhou HS, Li M, Sui BD, Wei L, Hou R, Chen WS, Li Q, Bi SH, Zhang JZ, Yi DH
Microvascular research11558-67(2018 Jan)

Human Connexin40 Mutations Slow Conduction and Increase Propensity for Atrial Fibrillation.
Kanthan A, Fahmy P, Rao R, Pouliopoulos J, Alexander IE, Thomas SP, Kizana E
Heart, lung & circulation27(1)114-121(2018 Jan)

Connexin40 correlates with oxidative stress in brains of traumatic brain injury rats.
Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W
Restorative neurology and neuroscience35(2)217-224(2017)

Specificity of the connexin W3/4 locus for functional gap junction formation.
Xu Q, Lin X, Matiukas A, Zhang X, Veenstra RD
Channels (Austin, Tex.)10(6)453-65(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated the Cx40 was upregulated after traumatic brain injury in a linear correlated manner with increased oxidative stress.
Title: Connexin40 correlates with oxidative stress in brains of traumatic brain injury rats.

data are consistent with contributions of G2 and D3 residues to Cx40 channel pore structure, but not with postulated hydrophobic W4 intermolecular interactions; results indicate an absolute requirement for an amphipathic W3/4 residue; alternatively hypothesize that the connexin D2/3-W3/4 locus interacts with the highly conserved FIFR M1 motif to stabilize the NT domain within the pore
Title: Specificity of the connexin W3/4 locus for functional gap junction formation.

Data show that the ratio of peripheral blood CD4(+)/CD8(+) T lymphocytes and the levels of connexin-40 (Cx40), interleukins IL-2, IL-4 and IL-6 are significantly elevated in SH rats.
Title: [An increase in the ratio of peripheral blood CD4+/CD8+ T lymphocytes and the levels of connexin 40 and inflammatory factors in spontaneously hypertensive rats].

Cx40 expression in mesangial cells is related to mesangial cell regeneration
Title: Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis.

The expressions of Cx40 and HCN4 increase obviously after coculturing bone marrow mesenchymal stem cells with sinoatrial node tissue.
Title: [Expression of connexin 40 and hyperpolarization-activated cyclic nucleotide-gated cation channel 4 in rat bone marrow mesenchymal stem cells cocultured with sinoatrial node tissues in vitro].

The following Gja5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gja5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04256	NM_019280.1 Latest version!	Rattus norvegicus gap junction protein, alpha 5 (Gja5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gja5 ( NM_019280.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062153.1 Rattus norvegicus gap junction protein, alpha 5 (Gja5), mRNA.

Price & Availability↑

CloneID	ORa04256
Clone ID Related Accession (Same CDS sequence)	NM_019280.1
Accession Version	NM_019280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	gap junction alpha-5 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF021806.1. On Jun 25, 2006 this sequence version replaced XM_001063632.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF021806.1, CK365277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGACT GGAGCTTCCT GGGGGAGTTC CTGGAGGAGG TCCACAAGCA CTCTACGGTC 
ATCGGCAAGG TCTGGCTCAC CGTCCTGTTC ATTTTCCGCA TGCTGGTCCT CGGCACCGCT 
GCTGAGTCCT CCTGGGGAGA TGAGCAGGCT GATTTCCGGT GTGATACCAT TCAGCCTGGT 
TGCCAAAATG TCTGCTACGA CCAAGCCTTC CCCATCTCCC ACATTCGTTA CTGGGTACTG 
CAGATCATCT TTGTGTCCAC ACCATCTCTG GTGTACATGG GCCACGCCAT GCACACTGTG 
CGCATGCAGG AAAAGCAGAA GCTGCGGGAG GCTGAGAAAG CTAAAGAGGC CGGTGGCACC 
GGCACCTATG AGTACTTGGC GGAGAAAGCC GAGCTGTCCT GCTGGAAAGA GGTGAACGGG 
AAGATTGTCC TCCAGGGCAC CCTACTCAAC ACCTATGTCT GCACCATTCT CATCCGCACC 
GCTATGGAGG TGGCCTTCAT GGTGGGCCAG TACCTCCTCT ATGGGATCTT CCTGGACACC 
CTGCATGTCT GCCGCAGAAG TCCCTGTCCC CACCCCGTCA ACTGTTATGT CTCGAGGCCC 
ACAGAGAAGA ACGTCTTCAT TGTCTTTATG ATGGCTGTGG CTGGACTGTC TCTGTTTCTC 
AGCCTGGCTG AACTCTACCA CCTGGGCTGG AAGAAGATCC GACAGCGCCT GGCCAAGTCA 
CGGCAGGGTG ACAAGCACCA GCTTCTTGGC CCCTCCACCA GCCTGGTCCA GGGCCTCACT 
CCTCCTCCTG ACTTCAACCA GTGCCTAAAG AACAGCCCAG ATGAGAAATT CTTCAGTGAC 
TTCAGTAATA ACATGGGCTC CCGGAAGAAT CCAGACCCTC TGGCCACTGA GGAAGTGCCA 
AACCAGGAGC AGATCCCGGA GGAAGGTTTC ATCCACACAC AGTATGGCCA GAAGCCCGAG 
CAGCCCAGTG GCGCCTCTGC AGGCCACCGC TTTCCTCAGG GCTACCACAG TGACAAGCGC 
CGCCTTAGTA AGGCCAGCAG CAAAGCAAGG TCAGATGACC TGTCAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062153.1
CDS	91..1161
Translation	MGDWSFLGEFLEEVHKHSTVIGKVWLTVLFIFRMLVLGTAAESSWGDEQADFRCDTIQPGCQNVCYDQAFPISHIRYWVLQIIFVSTPSLVYMGHAMHTVRMQEKQKLREAEKAKEAGGTGTYEYLAEKAELSCWKEVNGKIVLQGTLLNTYVCTILIRTAMEVAFMVGQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMMAVAGLSLFLSLAELYHLGWKKIRQRLAKSRQGDKHQLLGPSTSLVQGLTPPPDFNQCLKNSPDEKFFSDFSNNMGSRKNPDPLATEEVPNQEQIPEEGFIHTQYGQKPEQPSGASAGHRFPQGYHSDKRRLSKASSKARSDDLSV

Target ORF information:

RefSeq Version	NM_019280.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, alpha 5 (Gja5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019280.1

ORF Insert Sequence:

ATGGGTGACT GGAGCTTCCT GGGGGAGTTC CTGGAGGAGG TCCACAAGCA CTCTACGGTC 
ATCGGCAAGG TCTGGCTCAC CGTCCTGTTC ATTTTCCGCA TGCTGGTCCT CGGCACCGCT 
GCTGAGTCCT CCTGGGGAGA TGAGCAGGCT GATTTCCGGT GTGATACCAT TCAGCCTGGT 
TGCCAAAATG TCTGCTACGA CCAAGCCTTC CCCATCTCCC ACATTCGTTA CTGGGTACTG 
CAGATCATCT TTGTGTCCAC ACCATCTCTG GTGTACATGG GCCACGCCAT GCACACTGTG 
CGCATGCAGG AAAAGCAGAA GCTGCGGGAG GCTGAGAAAG CTAAAGAGGC CGGTGGCACC 
GGCACCTATG AGTACTTGGC GGAGAAAGCC GAGCTGTCCT GCTGGAAAGA GGTGAACGGG 
AAGATTGTCC TCCAGGGCAC CCTACTCAAC ACCTATGTCT GCACCATTCT CATCCGCACC 
GCTATGGAGG TGGCCTTCAT GGTGGGCCAG TACCTCCTCT ATGGGATCTT CCTGGACACC 
CTGCATGTCT GCCGCAGAAG TCCCTGTCCC CACCCCGTCA ACTGTTATGT CTCGAGGCCC 
ACAGAGAAGA ACGTCTTCAT TGTCTTTATG ATGGCTGTGG CTGGACTGTC TCTGTTTCTC 
AGCCTGGCTG AACTCTACCA CCTGGGCTGG AAGAAGATCC GACAGCGCCT GGCCAAGTCA 
CGGCAGGGTG ACAAGCACCA GCTTCTTGGC CCCTCCACCA GCCTGGTCCA GGGCCTCACT 
CCTCCTCCTG ACTTCAACCA GTGCCTAAAG AACAGCCCAG ATGAGAAATT CTTCAGTGAC 
TTCAGTAATA ACATGGGCTC CCGGAAGAAT CCAGACCCTC TGGCCACTGA GGAAGTGCCA 
AACCAGGAGC AGATCCCGGA GGAAGGTTTC ATCCACACAC AGTATGGCCA GAAGCCCGAG 
CAGCCCAGTG GCGCCTCTGC AGGCCACCGC TTTCCTCAGG GCTACCACAG TGACAAGCGC 
CGCCTTAGTA AGGCCAGCAG CAAAGCAAGG TCAGATGACC TGTCAGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lipopolysaccharide impairs permeability of pulmonary microvascular endothelial cells via Connexin40.
Microvascular research11558-67(2018 Jan)
Zhou HS,Li M,Sui BD,Wei L,Hou R,Chen WS,Li Q,Bi SH,Zhang JZ,Yi DH

Human Connexin40 Mutations Slow Conduction and Increase Propensity for Atrial Fibrillation.
Heart, lung & circulation27(1)114-121(2018 Jan)
Kanthan A,Fahmy P,Rao R,Pouliopoulos J,Alexander IE,Thomas SP,Kizana E

Connexin40 correlates with oxidative stress in brains of traumatic brain injury rats.
Restorative neurology and neuroscience35(2)217-224(2017)
Chen W,Guo Y,Yang W,Zheng P,Zeng J,Tong W

Specificity of the connexin W3/4 locus for functional gap junction formation.
Channels (Austin, Tex.)10(6)453-65(2016 Nov)
Xu Q,Lin X,Matiukas A,Zhang X,Veenstra RD

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Gja5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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