Srgap3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srgap3
Entrez Gene ID	500287
Full Name	SLIT-ROBO Rho GTPase activating protein 3
Synonyms	RGD1563152
General protein information	Preferred Names SLIT-ROBO Rho GTPase activating protein 3 Names SLIT-ROBO Rho GTPase-activating protein 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191975.1	NP_001178904.1	SLIT-ROBO Rho GTPase-activating protein 3
XM_006237048.3	XP_006237110.1	SLIT-ROBO Rho GTPase-activating protein 3 isoform X1
XM_008763196.2	XP_008761418.1	SLIT-ROBO Rho GTPase-activating protein 3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SRGAP3	NP_055665.1
Canis lupus familiaris (dog)	SRGAP3	XP_852764.1
Mus musculus (house mouse)	Srgap3	NP_536696.4
Gallus gallus (chicken)	SRGAP3	NP_001025775.1
Danio rerio (zebrafish)	srgap3	XP_005166172.1
Xenopus tropicalis (tropical clawed frog)	srgap3	XP_002936299.1
Pan troglodytes (chimpanzee)	SRGAP3	XP_003309646.2
Rattus norvegicus (Norway rat)	Srgap3	NP_001178904.1
Bos taurus (cattle)	SRGAP3	NP_001179895.1

Gene Ontology
Bibliography

Related articles in PubMed

The mental retardation-associated protein srGAP3 regulates survival, proliferation, and differentiation of rat embryonic neural stem/progenitor cells.
Lu H, Jiao Q, Wang Y, Yang Z, Feng M, Wang L, Chen X, Jin W, Liu Y
Stem cells and development22(11)1709-16(2013 Jun)

Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.
Yao Q, Jin WL, Wang Y, Ju G
Cellular and molecular neurobiology28(2)205-21(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results provide strong evidence that srGAP3 is involved in the regulation of biological behavior and the morphological features in neural stem cells/neural progenitor cells in vitro
Title: The mental retardation-associated protein srGAP3 regulates survival, proliferation, and differentiation of rat embryonic neural stem/progenitor cells.

results suggest that srGAPs are not only involved in the regulation of the Slit-Robo signal transduction, but also in neuronal development and that the translocation of srGAPs is important for their functions
Title: Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.

The following Srgap3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srgap3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa49331	XM_008763196.2 Latest version!	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa08228	NM_001191975.1 Latest version!	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa27576	XM_006237048.3 Latest version!	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srgap3 ( XM_008763196.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761418.1 Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa49331
Clone ID Related Accession (Same CDS sequence)	XM_008763196.2
Accession Version	XM_008763196.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGGCCC 
CCCTGTCTTC CCCCTAAACC ACAGAAAATG AGGAGACCTA GGCCTCTTTC AGTGTATAGC 
CATAAACTCT TTAACGGCAG TATGGAAGCG TTCATTAAGG ATTCAGGACA AGCTATACCA 
CTTGTAGTCG AGAGCTGTAT TCGATTCATC AATTTATATG GACTCCAGCA GCAGGGGATC 
TTCAGAGTTC CAGGGTCTCA GGTTGAAGTC AATGACATCA AGAATTCCTT TGAGAGAGGT 
GAAGACCCCC TCGTGGATGA CCAAAATGAG CGAGATATCA ATTCAGTTGC TGGTGTTTTA 
AAACTGTATT TCCGAGGACT GGAAAACCCA CTCTTTCCTA AGGAAAGGTT TCAAGATTTG 
ATATCTACTA TTAAACTGGA GAACCCAGCT GACAGGGTGC ACCCAATCCA GCAGATCCTC 
ATCACCCTGC CCCGCGTGGT CATTGTGGTC ATGAGATACC TCTTTGCTTT CCTCAATCAC 
CTTTCCCAGT ACAGTGATGA GAACATGATG GATCCCTACA ACTTGGCCAT CTGCTTCGGG 
CCCACCCTCA TGCACATCCC TGATGGGCAG GACCCTGTAT CCTGCCAGGC ACATGTCAAT 
GAAGTCATCA AAACCATCAT CATCCACCAT GAAGCCATCT TCCCCAGCCC GAGGGAGCTA 
GAGGGACCCG TATACGAAAA ATGCATGGCT GGAGGGGAAG AGTACTGTGA CAGCCCACAC 
AGTGAGCCGG GGACCATTGA TGAAGTTGAC CATGACAATG GCACGGAGCC TCATACCAGC 
GATGAAGAAG TGGAACAGAT CGAGGCCATC GCCAAGTTTG ACTATGTGGG GCGGTCCCCT 
CGTGAACTAT CTTTCAAAAA AGGGGCCTCA CTACTCCTGT ACCACCGTGC CTCCGAGGAC 
TGGTGGGAGG GCCGTCACAA TGGCGTAGAT GGACTCATCC CTCATCAGTA CATAGTTGTA 
CAGGACATGG ATGATGCCTT CTCCGATAGC CTGAGCCAAA AGGCTGACAG CGAGGCCAGC 
AGTGGGCCAC TGCTGGATGA CAAGGCCTCC TCCAAGAATG ACCTCCAGTC CCCCACAGAG 
CACATCTCTG ATTACGGCTT TGGGGGGGTG ATGGGCCGGA CATCGAGAAG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761418.1
CDS	837..3413
Translation	MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDIIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRFINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPADRVHPIQQILITLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTEHISDYGFGGVMGRTSRRP

Target ORF information:

RefSeq Version	XM_008763196.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763196.2

ORF Insert Sequence:

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGGCCC 
CCCTGTCTTC CCCCTAAACC ACAGAAAATG AGGAGACCTA GGCCTCTTTC AGTGTATAGC 
CATAAACTCT TTAACGGCAG TATGGAAGCG TTCATTAAGG ATTCAGGACA AGCTATACCA 
CTTGTAGTCG AGAGCTGTAT TCGATTCATC AATTTATATG GACTCCAGCA GCAGGGGATC 
TTCAGAGTTC CAGGGTCTCA GGTTGAAGTC AATGACATCA AGAATTCCTT TGAGAGAGGT 
GAAGACCCCC TCGTGGATGA CCAAAATGAG CGAGATATCA ATTCAGTTGC TGGTGTTTTA 
AAACTGTATT TCCGAGGACT GGAAAACCCA CTCTTTCCTA AGGAAAGGTT TCAAGATTTG 
ATATCTACTA TTAAACTGGA GAACCCAGCT GACAGGGTGC ACCCAATCCA GCAGATCCTC 
ATCACCCTGC CCCGCGTGGT CATTGTGGTC ATGAGATACC TCTTTGCTTT CCTCAATCAC 
CTTTCCCAGT ACAGTGATGA GAACATGATG GATCCCTACA ACTTGGCCAT CTGCTTCGGG 
CCCACCCTCA TGCACATCCC TGATGGGCAG GACCCTGTAT CCTGCCAGGC ACATGTCAAT 
GAAGTCATCA AAACCATCAT CATCCACCAT GAAGCCATCT TCCCCAGCCC GAGGGAGCTA 
GAGGGACCCG TATACGAAAA ATGCATGGCT GGAGGGGAAG AGTACTGTGA CAGCCCACAC 
AGTGAGCCGG GGACCATTGA TGAAGTTGAC CATGACAATG GCACGGAGCC TCATACCAGC 
GATGAAGAAG TGGAACAGAT CGAGGCCATC GCCAAGTTTG ACTATGTGGG GCGGTCCCCT 
CGTGAACTAT CTTTCAAAAA AGGGGCCTCA CTACTCCTGT ACCACCGTGC CTCCGAGGAC 
TGGTGGGAGG GCCGTCACAA TGGCGTAGAT GGACTCATCC CTCATCAGTA CATAGTTGTA 
CAGGACATGG ATGATGCCTT CTCCGATAGC CTGAGCCAAA AGGCTGACAG CGAGGCCAGC 
AGTGGGCCAC TGCTGGATGA CAAGGCCTCC TCCAAGAATG ACCTCCAGTC CCCCACAGAG 
CACATCTCTG ATTACGGCTT TGGGGGGGTG ATGGGCCGGA CATCGAGAAG ACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap3 ( NM_001191975.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178904.1 Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), mRNA.

Price & Availability↑

CloneID	ORa08228
Clone ID Related Accession (Same CDS sequence)	NM_001191975.1
Accession Version	NM_001191975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	SLIT-ROBO Rho GTPase-activating protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07061801.1, AABR07061800.1, AABR07061798.1 and AABR07061797.1. On Jul 17, 2010 this sequence version replaced XM_575637.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGGCCC 
CCCTGTCTTC CCCCTAAACC ACAGAAAATG AGGAGACCTA GGCCTCTTTC AGTGTATAGC 
CATAAACTCT TTAACGGCAG TATGGAAGCG TTCATTAAGG ATTCAGGACA AGCTATACCA 
CTTGTAGTCG AGAGCTGTAT TCGATTCATC AATTTATATG GACTCCAGCA GCAGGGGATC 
TTCAGAGTTC CAGGGTCTCA GGTTGAAGTC AATGACATCA AGAATTCCTT TGAGAGAGGT 
GAAGACCCCC TCGTGGATGA CCAAAATGAG CGAGATATCA ATTCAGTTGC TGGTGTTTTA 
AAACTGTATT TCCGAGGACT GGAAAACCCA CTCTTTCCTA AGGAAAGGTT TCAAGATTTG 
ATATCTACTA TTAAACTGGA GAACCCAGCT GACAGGGTGC ACCCAATCCA GCAGATCCTC 
ATCACCCTGC CCCGCGTGGT CATTGTGGTC ATGAGATACC TCTTTGCTTT CCTCAATCAC 
CTTTCCCAGT ACAGTGATGA GAACATGATG GATCCCTACA ACTTGGCCAT CTGCTTCGGG 
CCCACCCTCA TGCACATCCC TGATGGGCAG GACCCTGTAT CCTGCCAGGC ACATGTCAAT 
GAAGTCATCA AAACCATCAT CATCCACCAT GAAGCCATCT TCCCCAGCCC GAGGGAGCTA 
GAGGGACCCG TATACGAAAA ATGCATGGCT GGAGGGGAAG AGTACTGTGA CAGCCCACAC 
AGTGAGCCGG GGACCATTGA TGAAGTTGAC CATGACAATG GCACGGAGCC TCATACCAGC 
GATGAAGAAG TGGAACAGAT CGAGGCCATC GCCAAGTTTG ACTATGTGGG GCGGTCCCCT 
CGTGAACTAT CTTTCAAAAA AGGGGCCTCA CTACTCCTGT ACCACCGTGC CTCCGAGGAC 
TGGTGGGAGG GCCGTCACAA TGGCGTAGAT GGACTCATCC CTCATCAGTA CATAGTTGTA 
CAGGACATGG ATGATGCCTT CTCCGATAGC CTGAGCCAAA AGGCTGACAG CGAGGCCAGC 
AGTGGGCCAC TGCTGGATGA CAAGGCCTCC TCCAAGAATG ACCTCCAGTC CCCCACAGAG 
CACATCTCTG ATTACGGCTT TGGGGGGGTG ATGGGCCGAG TGAGACTACG GTCCGACGGA 
GCAGCCATCC CCAGGCGCCG AAGTGGGGGC GACACACACA GCCCACCCCG GGGCTTGGGT 
CCCAGCATAG ATACGCCACC CCGAGCTGCT GCCTGTCCCA GCAGCCCCCA CAAAATCCCC 
CTCAGCCGGG GACGGATTGA GAGCCCTGAG AAGAGGAGGA TGGCGACGTT CGGAAGCGCT 
GGCAGCATCA ACTACCCTGA CAAGAAGGCG CTGACAGAAG GGCTCTCCAT GAGGTCAACT 
TGCGGCTCCA CTCGACACAG CAGCCTAGGG GACCACAAGT CCCTAGAGGC TGAGGCCCTC 
GCAGAAGACA TCGAGAAGAC CATGAGCACG GCTCTTCATG AGTTGCGGGA ACTTGAGAGA 
CAGAACACTG TCAAGCAGGC ACCAGATGTG GTGCTGGACA CCCTGGAGCC CCTAAAGAAT 
CCACCAGGCC CCATCAGCTC AGAGCCTGCC AGCCCCCTGC ACACCATTGT CATCCGAGAC 
CCCGATGCTG CCATGCGTCG AAGCAGCAGC TCGTCTACAG AGATGATGAC CACCTTCAAG 
CCAGCCCTGT CGGCTCGCCT GGCTGGTGCC CAGCTGCGTC CACCCCCCAT GCGTCCAGTC 
AGACCTGTGG TCCAGCACCG GTCCAGCAGC AGCAGCAGCT CAGGTGTGGG CAGCCCGGCA 
GTGACACCTA CCGAGAAGAT GTTCCCTAAT AGCTCCTCAG ACAAGTCCGG CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178904.1
CDS	1..3300
Translation	MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDIIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRFINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPADRVHPIQQILITLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTEHISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLSRGRIESPEKRRMATFGSAGSINYPDKKALTEGLSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGPISSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSSDKSGTM

Target ORF information:

RefSeq Version	NM_001191975.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191975.1

ORF Insert Sequence:

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGGCCC 
CCCTGTCTTC CCCCTAAACC ACAGAAAATG AGGAGACCTA GGCCTCTTTC AGTGTATAGC 
CATAAACTCT TTAACGGCAG TATGGAAGCG TTCATTAAGG ATTCAGGACA AGCTATACCA 
CTTGTAGTCG AGAGCTGTAT TCGATTCATC AATTTATATG GACTCCAGCA GCAGGGGATC 
TTCAGAGTTC CAGGGTCTCA GGTTGAAGTC AATGACATCA AGAATTCCTT TGAGAGAGGT 
GAAGACCCCC TCGTGGATGA CCAAAATGAG CGAGATATCA ATTCAGTTGC TGGTGTTTTA 
AAACTGTATT TCCGAGGACT GGAAAACCCA CTCTTTCCTA AGGAAAGGTT TCAAGATTTG 
ATATCTACTA TTAAACTGGA GAACCCAGCT GACAGGGTGC ACCCAATCCA GCAGATCCTC 
ATCACCCTGC CCCGCGTGGT CATTGTGGTC ATGAGATACC TCTTTGCTTT CCTCAATCAC 
CTTTCCCAGT ACAGTGATGA GAACATGATG GATCCCTACA ACTTGGCCAT CTGCTTCGGG 
CCCACCCTCA TGCACATCCC TGATGGGCAG GACCCTGTAT CCTGCCAGGC ACATGTCAAT 
GAAGTCATCA AAACCATCAT CATCCACCAT GAAGCCATCT TCCCCAGCCC GAGGGAGCTA 
GAGGGACCCG TATACGAAAA ATGCATGGCT GGAGGGGAAG AGTACTGTGA CAGCCCACAC 
AGTGAGCCGG GGACCATTGA TGAAGTTGAC CATGACAATG GCACGGAGCC TCATACCAGC 
GATGAAGAAG TGGAACAGAT CGAGGCCATC GCCAAGTTTG ACTATGTGGG GCGGTCCCCT 
CGTGAACTAT CTTTCAAAAA AGGGGCCTCA CTACTCCTGT ACCACCGTGC CTCCGAGGAC 
TGGTGGGAGG GCCGTCACAA TGGCGTAGAT GGACTCATCC CTCATCAGTA CATAGTTGTA 
CAGGACATGG ATGATGCCTT CTCCGATAGC CTGAGCCAAA AGGCTGACAG CGAGGCCAGC 
AGTGGGCCAC TGCTGGATGA CAAGGCCTCC TCCAAGAATG ACCTCCAGTC CCCCACAGAG 
CACATCTCTG ATTACGGCTT TGGGGGGGTG ATGGGCCGAG TGAGACTACG GTCCGACGGA 
GCAGCCATCC CCAGGCGCCG AAGTGGGGGC GACACACACA GCCCACCCCG GGGCTTGGGT 
CCCAGCATAG ATACGCCACC CCGAGCTGCT GCCTGTCCCA GCAGCCCCCA CAAAATCCCC 
CTCAGCCGGG GACGGATTGA GAGCCCTGAG AAGAGGAGGA TGGCGACGTT CGGAAGCGCT 
GGCAGCATCA ACTACCCTGA CAAGAAGGCG CTGACAGAAG GGCTCTCCAT GAGGTCAACT 
TGCGGCTCCA CTCGACACAG CAGCCTAGGG GACCACAAGT CCCTAGAGGC TGAGGCCCTC 
GCAGAAGACA TCGAGAAGAC CATGAGCACG GCTCTTCATG AGTTGCGGGA ACTTGAGAGA 
CAGAACACTG TCAAGCAGGC ACCAGATGTG GTGCTGGACA CCCTGGAGCC CCTAAAGAAT 
CCACCAGGCC CCATCAGCTC AGAGCCTGCC AGCCCCCTGC ACACCATTGT CATCCGAGAC 
CCCGATGCTG CCATGCGTCG AAGCAGCAGC TCGTCTACAG AGATGATGAC CACCTTCAAG 
CCAGCCCTGT CGGCTCGCCT GGCTGGTGCC CAGCTGCGTC CACCCCCCAT GCGTCCAGTC 
AGACCTGTGG TCCAGCACCG GTCCAGCAGC AGCAGCAGCT CAGGTGTGGG CAGCCCGGCA 
GTGACACCTA CCGAGAAGAT GTTCCCTAAT AGCTCCTCAG ACAAGTCCGG CACTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap3 ( XM_006237048.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237110.1 Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X1, mRNA.

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CloneID	ORa27576
Clone ID Related Accession (Same CDS sequence)	XM_006237048.3
Accession Version	XM_006237048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SLIT-ROBO Rho GTPase-activating protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGAGGA 
AGAAGAAATG CTCGAACCAG GAACCAGGAT TCAGGACAAG CTATACCACT TGTAGTCGAG 
AGCTGTATTC GATTCATCAA TTTATATGGA CTCCAGCAGC AGGGGATCTT CAGAGTTCCA 
GGGTCTCAGG TTGAAGTCAA TGACATCAAG AATTCCTTTG AGAGAGGTGA AGACCCCCTC 
GTGGATGACC AAAATGAGCG AGATATCAAT TCAGTTGCTG GTGTTTTAAA ACTGTATTTC 
CGAGGACTGG AAAACCCACT CTTTCCTAAG GAAAGGTTTC AAGATTTGAT ATCTACTATT 
AAACTGGAGA ACCCAGCTGA CAGGGTGCAC CCAATCCAGC AGATCCTCAT CACCCTGCCC 
CGCGTGGTCA TTGTGGTCAT GAGATACCTC TTTGCTTTCC TCAATCACCT TTCCCAGTAC 
AGTGATGAGA ACATGATGGA TCCCTACAAC TTGGCCATCT GCTTCGGGCC CACCCTCATG 
CACATCCCTG ATGGGCAGGA CCCTGTATCC TGCCAGGCAC ATGTCAATGA AGTCATCAAA 
ACCATCATCA TCCACCATGA AGCCATCTTC CCCAGCCCGA GGGAGCTAGA GGGACCCGTA 
TACGAAAAAT GCATGGCTGG AGGGGAAGAG TACTGTGACA GCCCACACAG TGAGCCGGGG 
ACCATTGATG AAGTTGACCA TGACAATGGC ACGGAGCCTC ATACCAGCGA TGAAGAAGTG 
GAACAGATCG AGGCCATCGC CAAGTTTGAC TATGTGGGGC GGTCCCCTCG TGAACTATCT 
TTCAAAAAAG GGGCCTCACT ACTCCTGTAC CACCGTGCCT CCGAGGACTG GTGGGAGGGC 
CGTCACAATG GCGTAGATGG ACTCATCCCT CATCAGTACA TAGTTGTACA GGACATGGAT 
GATGCCTTCT CCGATAGCCT GAGCCAAAAG GCTGACAGCG AGGCCAGCAG TGGGCCACTG 
CTGGATGACA AGGCCTCCTC CAAGAATGAC CTCCAGTCCC CCACAGAGCA CATCTCTGAT 
TACGGCTTTG GGGGGGTGAT GGGCCGAGTG AGACTACGGT CCGACGGAGC AGCCATCCCC 
AGGCGCCGAA GTGGGGGCGA CACACACAGC CCACCCCGGG GCTTGGGTCC CAGCATAGAT 
ACGCCACCCC GAGCTGCTGC CTGTCCCAGC AGCCCCCACA AAATCCCCCT CAGCCGGGGA 
CGGATTGAGA GCCCTGAGAA GAGGAGGATG GCGACGTTCG GAAGCGCTGG CAGCATCAAC 
TACCCTGACA AGAAGGCGCT GACAGAAGGG CTCTCCATGA GGTCAACTTG CGGCTCCACT 
CGACACAGCA GCCTAGGGGA CCACAAGTCC CTAGAGGCTG AGGCCCTCGC AGAAGACATC 
GAGAAGACCA TGAGCACGGC TCTTCATGAG TTGCGGGAAC TTGAGAGACA GAACACTGTC 
AAGCAGGCAC CAGATGTGGT GCTGGACACC CTGGAGCCCC TAAAGAATCC ACCAGGCCCC 
ATCAGCTCAG AGCCTGCCAG CCCCCTGCAC ACCATTGTCA TCCGAGACCC CGATGCTGCC 
ATGCGTCGAA GCAGCAGCTC GTCTACAGAG ATGATGACCA CCTTCAAGCC AGCCCTGTCG 
GCTCGCCTGG CTGGTGCCCA GCTGCGTCCA CCCCCCATGC GTCCAGTCAG ACCTGTGGTC 
CAGCACCGGT CCAGCAGCAG CAGCAGCTCA GGTGTGGGCA GCCCGGCAGT GACACCTACC 
GAGAAGATGT TCCCTAATAG CTCCTCAGAC AAGTCCGGCA CTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237110.1
CDS	837..4064
Translation	MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLDIIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRGRRNARTRNQDSGQAIPLVVESCIRFINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPADRVHPIQQILITLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTEHISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLSRGRIESPEKRRMATFGSAGSINYPDKKALTEGLSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGPISSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSSDKSGTM

Target ORF information:

RefSeq Version	XM_006237048.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237048.3

ORF Insert Sequence:

ATGTCATCTC AAACTAAGTT CAAGAAAGAC AAAGAGATCA TTGCTGAATA TGAAGCCCAA 
ATAAAAGAGA TCCGCACGCA GCTGGTGGAG CAGTTCAAAT GCCTGGAGCA GCAGTCAGAG 
TCGCGGCTGC AGCTGCTGCA AGACTTGCAG GAGTTCTTCC GCAGGAAAGC TGAGATCGAG 
CTGGAGTATT CCCGCAGCCT GGAGAAGCTG GCCGAGCGCT TCTCCTCCAA GATTCGAAGC 
TCCCGGGAGC ACCAGTTCAA GAAGGATCAA TACCTCCTCT CACCTGTGAA CTGTTGGTAT 
CTGGTACTGC ATCAGACTCG GCGGGAGAGC CGAGATCACG CCACCCTCAA TGACATCTTC 
ATGAACAATG TCATCGTCCG CCTCTCCCAG ATCAGTGAAG ATGTCATCAG ACTCTTCAAA 
AAGAGCAAAG AGATTGGCCT GCAGATGCAT GAGGAGCTGC TGAAGGTGAC CAATGAGCTC 
TACACAGTCA TGAAAACCTA CCACATGTAC CATGCAGAGA GCATCAGTGC AGAAAGCAAG 
CTGAAGGAGG CCGAGAAGCA GGAGGAGAAA CAGTTTAACA AGTCAGGAGA CCTGAGCATG 
AACCTTCTCC GGCACGAGGA CAGACCCCAG CGCCGCAGCT CCGTGAAGAA GATTGAGAAG 
ATGAAGGAGA AGAGGCAGGC CAAGTATTCT GAGAACAAGC TCAAGTGCAC AAAGGCCCGA 
AATGACTACC TGCTGAATCT GGCAGCCACC AACGCAGCTA TCAGCAAATA CTACATCCAC 
GATGTCTCTG ATCTCATCGA TTGCTGTGAT TTGGGCTTTC ACGCCAGCCT GGCCCGCACC 
TTTCGGACCT ACCTCTCAGC AGAATATAAC CTGGAGACCT CTCGCCACGA GGGCCTGGAC 
ATCATAGAGA ATGCAGTGGA CAACCTGGAC TCCCGGAGTG ACAAGCACAC AGTCATGGAC 
ATGTGCAGCC AGGTCTTCTG CCCTCCACTC AAGTTTGAGT TCCAGCCCCA CATGGGGGAT 
GAGGTCTGCC AGGTCAGTGC ACAGCAGCCT GTCCAGACGG AGCTGCTTAT GCGGTACCAC 
CAGCTGCAGT CCAGGCTGGC CACTCTCAAG ATTGAGAACG AAGAGGTTAG GAAAACCCTG 
GATGCCACCA TGCAGACCTT ACAGGACATG CTGACCGTGG AGGACTTTGA TGTCTCTGAC 
GCCTTCCAAC ACAGCCGATC TACAGAGTCC ATAAAGTCAG CTGCTTCTGA GACCTACATG 
AGCAAAATCA ACATCGCCAA AAGGAGAGCC AACCAGCAGG AGACAGAAAT GTTTTATTTT 
ACAAAATTTA AAGAATATGT GAATGGCAGT AACCTCATCA CCAAGCTGCA AGCCAAGCAT 
GACTTACTCA AGCAGACCCT GGGTGAAGGG GAGAGAGCAG AGTGCGGCAC AACCAGAGGA 
AGAAGAAATG CTCGAACCAG GAACCAGGAT TCAGGACAAG CTATACCACT TGTAGTCGAG 
AGCTGTATTC GATTCATCAA TTTATATGGA CTCCAGCAGC AGGGGATCTT CAGAGTTCCA 
GGGTCTCAGG TTGAAGTCAA TGACATCAAG AATTCCTTTG AGAGAGGTGA AGACCCCCTC 
GTGGATGACC AAAATGAGCG AGATATCAAT TCAGTTGCTG GTGTTTTAAA ACTGTATTTC 
CGAGGACTGG AAAACCCACT CTTTCCTAAG GAAAGGTTTC AAGATTTGAT ATCTACTATT 
AAACTGGAGA ACCCAGCTGA CAGGGTGCAC CCAATCCAGC AGATCCTCAT CACCCTGCCC 
CGCGTGGTCA TTGTGGTCAT GAGATACCTC TTTGCTTTCC TCAATCACCT TTCCCAGTAC 
AGTGATGAGA ACATGATGGA TCCCTACAAC TTGGCCATCT GCTTCGGGCC CACCCTCATG 
CACATCCCTG ATGGGCAGGA CCCTGTATCC TGCCAGGCAC ATGTCAATGA AGTCATCAAA 
ACCATCATCA TCCACCATGA AGCCATCTTC CCCAGCCCGA GGGAGCTAGA GGGACCCGTA 
TACGAAAAAT GCATGGCTGG AGGGGAAGAG TACTGTGACA GCCCACACAG TGAGCCGGGG 
ACCATTGATG AAGTTGACCA TGACAATGGC ACGGAGCCTC ATACCAGCGA TGAAGAAGTG 
GAACAGATCG AGGCCATCGC CAAGTTTGAC TATGTGGGGC GGTCCCCTCG TGAACTATCT 
TTCAAAAAAG GGGCCTCACT ACTCCTGTAC CACCGTGCCT CCGAGGACTG GTGGGAGGGC 
CGTCACAATG GCGTAGATGG ACTCATCCCT CATCAGTACA TAGTTGTACA GGACATGGAT 
GATGCCTTCT CCGATAGCCT GAGCCAAAAG GCTGACAGCG AGGCCAGCAG TGGGCCACTG 
CTGGATGACA AGGCCTCCTC CAAGAATGAC CTCCAGTCCC CCACAGAGCA CATCTCTGAT 
TACGGCTTTG GGGGGGTGAT GGGCCGAGTG AGACTACGGT CCGACGGAGC AGCCATCCCC 
AGGCGCCGAA GTGGGGGCGA CACACACAGC CCACCCCGGG GCTTGGGTCC CAGCATAGAT 
ACGCCACCCC GAGCTGCTGC CTGTCCCAGC AGCCCCCACA AAATCCCCCT CAGCCGGGGA 
CGGATTGAGA GCCCTGAGAA GAGGAGGATG GCGACGTTCG GAAGCGCTGG CAGCATCAAC 
TACCCTGACA AGAAGGCGCT GACAGAAGGG CTCTCCATGA GGTCAACTTG CGGCTCCACT 
CGACACAGCA GCCTAGGGGA CCACAAGTCC CTAGAGGCTG AGGCCCTCGC AGAAGACATC 
GAGAAGACCA TGAGCACGGC TCTTCATGAG TTGCGGGAAC TTGAGAGACA GAACACTGTC 
AAGCAGGCAC CAGATGTGGT GCTGGACACC CTGGAGCCCC TAAAGAATCC ACCAGGCCCC 
ATCAGCTCAG AGCCTGCCAG CCCCCTGCAC ACCATTGTCA TCCGAGACCC CGATGCTGCC 
ATGCGTCGAA GCAGCAGCTC GTCTACAGAG ATGATGACCA CCTTCAAGCC AGCCCTGTCG 
GCTCGCCTGG CTGGTGCCCA GCTGCGTCCA CCCCCCATGC GTCCAGTCAG ACCTGTGGTC 
CAGCACCGGT CCAGCAGCAG CAGCAGCTCA GGTGTGGGCA GCCCGGCAGT GACACCTACC 
GAGAAGATGT TCCCTAATAG CTCCTCAGAC AAGTCCGGCA CTATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mental retardation-associated protein srGAP3 regulates survival, proliferation, and differentiation of rat embryonic neural stem/progenitor cells.
Stem cells and development22(11)1709-16(2013 Jun)
Lu H,Jiao Q,Wang Y,Yang Z,Feng M,Wang L,Chen X,Jin W,Liu Y

Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.
Cellular and molecular neurobiology28(2)205-21(2008 Feb)
Yao Q,Jin WL,Wang Y,Ju G

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Srgap3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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