Ppp1r3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r3a
Entrez Gene ID	500036
Full Name	protein phosphatase 1, regulatory subunit 3A
Synonyms	RGD1561312
General protein information	Preferred Names protein phosphatase 1, regulatory subunit 3A Names protein phosphatase 1 regulatory subunit 3A protein phosphatase 1, regulatory (inhibitor) subunit 3A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109222.1	NP_001102692.1	protein phosphatase 1 regulatory subunit 3A
XM_008762717.2	XP_008760939.1	protein phosphatase 1 regulatory subunit 3A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1R3A	NP_002702.2
Canis lupus familiaris (dog)	PPP1R3A	XP_539528.2
Pan troglodytes (chimpanzee)	PPP1R3A	XP_519320.3
	PPP1R3A	XP_001100958.2
Bos taurus (cattle)	PPP1R3A	NP_001192369.1
Mus musculus (house mouse)	Ppp1r3a	NP_536712.2
Rattus norvegicus (Norway rat)	Ppp1r3a	NP_001102692.1
Gallus gallus (chicken)	PPP1R3A	XP_004937598.1

Gene Ontology
Bibliography

Related articles in PubMed

Rg1 prevents myocardial hypoxia/reoxygenation injury by regulating mitochondrial dynamics imbalance via modulation of glutamate dehydrogenase and mitofusin 2.
Dong G, Chen T, Ren X, Zhang Z, Huang W, Liu L, Luo P, Zhou H
Mitochondrion267-18(2016 Jan)

Functional diversity of protein phosphatase-1, a cellular economizer and reset button.
Ceulemans H, Bollen M
Physiological reviews84(1)1-39(2004 Jan)

Male preponderance in early diagnosed type 2 diabetes is associated with the ARE insertion/deletion polymorphism in the PPP1R3A locus.
Doney AS, Fischer B, Cecil JE, Cohen PT, Boyle DI, Leese G, Morris AD, Palmer CN
BMC genetics411(2003 Jun)

Digenic inheritance of severe insulin resistance in a human pedigree.
Savage DB, Agostini M, Barroso I, Gurnell M, Luan J, Meirhaeghe A, Harding AH, Ihrke G, Rajanayagam O, Soos MA, George S, Berger D, Thomas EL, Bell JD, Meeran K, Ross RJ, Vidal-Puig A, Wareham NJ, O'Rahilly S, Chatterjee VK, Schafer AJ
Nature genetics31(4)379-84(2002 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rg1 through modulation of GDH and MFN2 maintained mitochondrial dynamics that resulted in protection against H/R-induced cardiomyocyte injury. All these results put forward a new protective mechanism of Rg1 on the therapeutic potential in cardiac I/R disorders.
Title: Rg1 prevents myocardial hypoxia/reoxygenation injury by regulating mitochondrial dynamics imbalance via modulation of glutamate dehydrogenase and mitofusin 2.

The following Ppp1r3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09361	NM_001109222.1 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa49298	XM_008762717.2 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r3a ( NM_001109222.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102692.1 Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), mRNA.

Price & Availability↑

CloneID	ORa09361
Clone ID Related Accession (Same CDS sequence)	NM_001109222.1
Accession Version	NM_001109222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 3A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473959.1. On or before Oct 4, 2007 this sequence version replaced XM_575392.2, XM_001057957.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689602, SAMN02642627 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCG CCGAAGTACC CGGTCAGATT AGCAAAGATA ACTTTTTAGA AGTTCCTAAT 
CTGTCCGATT CTGTGTGTGA AGATGAAGAA GTTAAAGCGA CCTTCAAGCC TGGCTTCTCC 
CCTCAGCCAA GCAGACGGGG GTCAGAATCT TCTGAAGACT TATATCTGGA CACCCCAACC 
TCAGCTTCCC GGAGAGTTTC CTTTGCTGAC AGCCTTGGAT TCAGTCTTGT GTCTGTTAAA 
GAATTTGATT GTTGGGACTT ACCAAGTGTT TCAACTGATT TTGACTTAAC TAGGGATGTC 
CTCCACACAG ATGAATATAT TTTATCTCCA CTGTTTGACT TGCCTTCTTC CAAAGAAGAG 
CTTATGGAAC AACTTCAAGT CCAGAAGGCA GTGCTGGAGT CGGCTGAACA CCTTCCTGGG 
TCGACAAGTG TGAAGGGCAT CATTCGAGTT CTGAACATTT CTTTTGAGAA GTTAGTGTAT 
GTGCGCATGT CTTTGGACGA CTGGCAGACA CATTATGACA TTTTAGCAGA ATATGTTCCT 
AATTCGTGCG ATGGTGAAAC TGACCAGTTC TCCTTTAAGA TTTCATTGGT TCCACCTTAT 
CAAATGGACG GCGGTAAAGT GGAGTTTTGC ATACGCTATG AAACTTCTGT TGGTACATTT 
TGGTCAAATA ATAATGGGAC AAATTATATA TTGGTTTGTC AAAAGAAAAC AAAGGAGCCA 
GAGCCTGTAG TAAAGCCACT GGAAGAAGGT CCTAGTAGAC AAATAAAAGG CTGCTTAAAG 
GTAAAATCAA GCAGTAAAGA AGAACCATTG CTAACACCAG AAGAAAATAA GCTTGGGACT 
TTGAAGTTCA CAGAGTCCTA CATCCCAACC ATTATTTGTT CTCATGAGGA CAGGGATGAC 
TTGGGAGCCA ATTATCAACA TGTAAAAGAC ATCGACAAGA AGCATGATGA ACATAATGAA 
AAGGAATTAG ACTTGATGAT AAACCAACGG TTAATAACTT CCCATGACGA AAAGAATACA 
TTTTCAACAG ATGCAGTCAA CTCCACAAAT AAAGCTGAGG GGTCAGAGAA GAAACAAGCT 
CGCCATGAAA TCCACACTGA CTTGTGCAGA GGGCCTTTGT CTCCGAGTTT ATCAGCAGAA 
AGTTCTTTAA AGAGAGATTT CTACCACAGC AGAAATTATT CCCCAGGAAA TGAGCATGGT 
CATCCACCTT CAGAGGAAAT TATTTCGGAT GTGGGAGCGA TAGGGTCACC ACTGGGAGAT 
ACTAGTTCTG ATGAGTTAAT GCAACTGAAA GTCTGCAGCA AAGAAGACGT AGACGATAAT 
GCCAACCCAG CCCACGGGAG TGGCAGATTG TGTTCTTCCT CTGATCAACT AAGGGCAGGT 
GGTCTTGAGA ATAATGAAGC AGGAATCAAG AAAACTGGAA TAAAAGATTA CAAATATTCA 
CACGGAGATT CTTATTTAGA GGCATCCACA AGTTTGAGAG AATCAAATGC GTCTTACAAA 
GATGAGTACG ACAAAGTGGA TGATAAAAGG GAGAAGAAAA CATGCTTAGG TGTTAATGAA 
AACCCAGGCA AGAATTTTCA ATCAATATTC CAGAACCAAG AGAGACAAAT GGGGTGTGCA 
AAAATAAGCG GTGAAGGGGC TAATGCTAAT AACCAAGACT TGACCACTCT ACTGAGCAAA 
GATACCACAA CTAACACCTG GGCGGTAACC GTCGATCCCT CTCCTTCAAC AAATACAAAC 
GTGAATTGGA GAGAAGCCGG AAATGGTACC GATTTAGAAC GAAGAACTAC TGATCTTGGT 
TCTCCAAGGA ATTTCAGTCT CCTGACTGAT GGTTATCTTT TTCAAGCTGA CAGGGAAAAA 
TCCGACTCTA GTAATTCTGA AGATCGGAAT ATGAACCCAC AGCACAAAAA AAATTGGAAC 
GTTCTGGAAA GCCAGCCAGA AACAAGAGAG ACTGAAACAA ATATAACCAA GCATACCAAA 
GAACAGGCAG AGTACAAAGA CACGTGGGGA AAAAGAGATA ACAGTAGGAA TCTGAAAGCT 
ACTTCTACAG AGCAATTGTT TACGTGCCAA GAAACAGAAT GCTGTGAACT GTCTTCTCTA 
GCTGATCATG GTATCACTGA GAAAGCACAA GCAGTTGCAG CTTATATAAT TAAGACAACA 
TTAGAAAGTA CTCCAGAAAG CGCGTCTGCT AGAGGAAAAG CAATAATTGC TAAGCTACCT 
CAAGAGACAG CACGACATGA CAGGCCCATC GAGGTAAAAG AAACAGCGTT TGATCCACAT 
GAAGGGCGGA AGGATGATTC ACATTATTCC CTTTGTCATG GAGATACAGC AGGTGTAATC 
CATGACAATG ATTTTGAAAG GGAGTCACAT TTAGATATTT GTAATGTACG TGTAGATGAA 
ATGGAGAAGG AGAAAACCAC ATCTACGTGC TCTCCTAGGA AGACATATGA CAAGGAGAAA 
CATGGAATTG GAAGTGTAAC ATCTATAGAC GAACCTTCAC AGGTCATTAC AGGCAATCAA 
AAAGCTACGT CAAAACTGGA TTTACATTTG GGAGTGTTAC CAACAGACAG AGCAATACTC 
TCGGCAAACG CAGATCTTGA GCTGCTTCAA GAATTATCAA GGAGAACAGA CTTCAATGCT 
GTTCACTCTG CATTTAACTC AGACCCTGGC AGCGCTTCTC AGGACAGCTC TCAAGTTTAC 
AGACGCTGCT CTAAAAAACC AGTACCTTCT TATGGTGAAC AGAAAGCAGT TATTAACACA 
ACACTCCAGT CTGTTCCTAC GAAATCAGAA TATAATTGTC ACCCAGAAAG TGAGATCTTC 
GGTCATGCCA CGTCTAAACC GGAAGTTGTT TTCAAAAGTT CAGAAATAAT AAAATCAGGT 
AGTGGAGGAG AGCAAGGAGT GGGTCCGATT CTCCAACAAA AAGAAGGTAG TTTGGAAAAA 
TCCCAAGACC CGATGATTTC AATCAACGAG CCTCTTGAGA ATCTGGATGA AGTACGTTCT 
GAAAATGAAG GGTTAATGCG TTCTGGTCAG TTACAGTGTT ATTTGGGTGA CAAGGGATCA 
GTGAGTTCTG CTTCTGCTAC TGTCCCTACC CAGGAACTCC AAGCTCAAGG CAGCGAGTCT 
CTGCTTTCAA TATCCATCAA CTCTAAAATA CCTTATTTCC TTTTGTTTCT AATATTTCTT 
GCAACTGTCT ACTACTATGA CCTAATGATA GGCTTGGCAT TCTATCTTTT TTCCTTGTAC 
TGGTTGTACT GGGAAGGGGG GAGGCAAAGA GAGTCTGTCA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102692.1
CDS	61..3351
Translation	MEPAEVPGQISKDNFLEVPNLSDSVCEDEEVKATFKPGFSPQPSRRGSESSEDLYLDTPTSASRRVSFADSLGFSLVSVKEFDCWDLPSVSTDFDLTRDVLHTDEYILSPLFDLPSSKEELMEQLQVQKAVLESAEHLPGSTSVKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQMDGGKVEFCIRYETSVGTFWSNNNGTNYILVCQKKTKEPEPVVKPLEEGPSRQIKGCLKVKSSSKEEPLLTPEENKLGTLKFTESYIPTIICSHEDRDDLGANYQHVKDIDKKHDEHNEKELDLMINQRLITSHDEKNTFSTDAVNSTNKAEGSEKKQARHEIHTDLCRGPLSPSLSAESSLKRDFYHSRNYSPGNEHGHPPSEEIISDVGAIGSPLGDTSSDELMQLKVCSKEDVDDNANPAHGSGRLCSSSDQLRAGGLENNEAGIKKTGIKDYKYSHGDSYLEASTSLRESNASYKDEYDKVDDKREKKTCLGVNENPGKNFQSIFQNQERQMGCAKISGEGANANNQDLTTLLSKDTTTNTWAVTVDPSPSTNTNVNWREAGNGTDLERRTTDLGSPRNFSLLTDGYLFQADREKSDSSNSEDRNMNPQHKKNWNVLESQPETRETETNITKHTKEQAEYKDTWGKRDNSRNLKATSTEQLFTCQETECCELSSLADHGITEKAQAVAAYIIKTTLESTPESASARGKAIIAKLPQETARHDRPIEVKETAFDPHEGRKDDSHYSLCHGDTAGVIHDNDFERESHLDICNVRVDEMEKEKTTSTCSPRKTYDKEKHGIGSVTSIDEPSQVITGNQKATSKLDLHLGVLPTDRAILSANADLELLQELSRRTDFNAVHSAFNSDPGSASQDSSQVYRRCSKKPVPSYGEQKAVINTTLQSVPTKSEYNCHPESEIFGHATSKPEVVFKSSEIIKSGSGGEQGVGPILQQKEGSLEKSQDPMISINEPLENLDEVRSENEGLMRSGQLQCYLGDKGSVSSASATVPTQELQAQGSESLLSISINSKIPYFLLFLIFLATVYYYDLMIGLAFYLFSLYWLYWEGGRQRESVKKK

Target ORF information:

RefSeq Version	NM_001109222.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109222.1

ORF Insert Sequence:

ATGGAGCCCG CCGAAGTACC CGGTCAGATT AGCAAAGATA ACTTTTTAGA AGTTCCTAAT 
CTGTCCGATT CTGTGTGTGA AGATGAAGAA GTTAAAGCGA CCTTCAAGCC TGGCTTCTCC 
CCTCAGCCAA GCAGACGGGG GTCAGAATCT TCTGAAGACT TATATCTGGA CACCCCAACC 
TCAGCTTCCC GGAGAGTTTC CTTTGCTGAC AGCCTTGGAT TCAGTCTTGT GTCTGTTAAA 
GAATTTGATT GTTGGGACTT ACCAAGTGTT TCAACTGATT TTGACTTAAC TAGGGATGTC 
CTCCACACAG ATGAATATAT TTTATCTCCA CTGTTTGACT TGCCTTCTTC CAAAGAAGAG 
CTTATGGAAC AACTTCAAGT CCAGAAGGCA GTGCTGGAGT CGGCTGAACA CCTTCCTGGG 
TCGACAAGTG TGAAGGGCAT CATTCGAGTT CTGAACATTT CTTTTGAGAA GTTAGTGTAT 
GTGCGCATGT CTTTGGACGA CTGGCAGACA CATTATGACA TTTTAGCAGA ATATGTTCCT 
AATTCGTGCG ATGGTGAAAC TGACCAGTTC TCCTTTAAGA TTTCATTGGT TCCACCTTAT 
CAAATGGACG GCGGTAAAGT GGAGTTTTGC ATACGCTATG AAACTTCTGT TGGTACATTT 
TGGTCAAATA ATAATGGGAC AAATTATATA TTGGTTTGTC AAAAGAAAAC AAAGGAGCCA 
GAGCCTGTAG TAAAGCCACT GGAAGAAGGT CCTAGTAGAC AAATAAAAGG CTGCTTAAAG 
GTAAAATCAA GCAGTAAAGA AGAACCATTG CTAACACCAG AAGAAAATAA GCTTGGGACT 
TTGAAGTTCA CAGAGTCCTA CATCCCAACC ATTATTTGTT CTCATGAGGA CAGGGATGAC 
TTGGGAGCCA ATTATCAACA TGTAAAAGAC ATCGACAAGA AGCATGATGA ACATAATGAA 
AAGGAATTAG ACTTGATGAT AAACCAACGG TTAATAACTT CCCATGACGA AAAGAATACA 
TTTTCAACAG ATGCAGTCAA CTCCACAAAT AAAGCTGAGG GGTCAGAGAA GAAACAAGCT 
CGCCATGAAA TCCACACTGA CTTGTGCAGA GGGCCTTTGT CTCCGAGTTT ATCAGCAGAA 
AGTTCTTTAA AGAGAGATTT CTACCACAGC AGAAATTATT CCCCAGGAAA TGAGCATGGT 
CATCCACCTT CAGAGGAAAT TATTTCGGAT GTGGGAGCGA TAGGGTCACC ACTGGGAGAT 
ACTAGTTCTG ATGAGTTAAT GCAACTGAAA GTCTGCAGCA AAGAAGACGT AGACGATAAT 
GCCAACCCAG CCCACGGGAG TGGCAGATTG TGTTCTTCCT CTGATCAACT AAGGGCAGGT 
GGTCTTGAGA ATAATGAAGC AGGAATCAAG AAAACTGGAA TAAAAGATTA CAAATATTCA 
CACGGAGATT CTTATTTAGA GGCATCCACA AGTTTGAGAG AATCAAATGC GTCTTACAAA 
GATGAGTACG ACAAAGTGGA TGATAAAAGG GAGAAGAAAA CATGCTTAGG TGTTAATGAA 
AACCCAGGCA AGAATTTTCA ATCAATATTC CAGAACCAAG AGAGACAAAT GGGGTGTGCA 
AAAATAAGCG GTGAAGGGGC TAATGCTAAT AACCAAGACT TGACCACTCT ACTGAGCAAA 
GATACCACAA CTAACACCTG GGCGGTAACC GTCGATCCCT CTCCTTCAAC AAATACAAAC 
GTGAATTGGA GAGAAGCCGG AAATGGTACC GATTTAGAAC GAAGAACTAC TGATCTTGGT 
TCTCCAAGGA ATTTCAGTCT CCTGACTGAT GGTTATCTTT TTCAAGCTGA CAGGGAAAAA 
TCCGACTCTA GTAATTCTGA AGATCGGAAT ATGAACCCAC AGCACAAAAA AAATTGGAAC 
GTTCTGGAAA GCCAGCCAGA AACAAGAGAG ACTGAAACAA ATATAACCAA GCATACCAAA 
GAACAGGCAG AGTACAAAGA CACGTGGGGA AAAAGAGATA ACAGTAGGAA TCTGAAAGCT 
ACTTCTACAG AGCAATTGTT TACGTGCCAA GAAACAGAAT GCTGTGAACT GTCTTCTCTA 
GCTGATCATG GTATCACTGA GAAAGCACAA GCAGTTGCAG CTTATATAAT TAAGACAACA 
TTAGAAAGTA CTCCAGAAAG CGCGTCTGCT AGAGGAAAAG CAATAATTGC TAAGCTACCT 
CAAGAGACAG CACGACATGA CAGGCCCATC GAGGTAAAAG AAACAGCGTT TGATCCACAT 
GAAGGGCGGA AGGATGATTC ACATTATTCC CTTTGTCATG GAGATACAGC AGGTGTAATC 
CATGACAATG ATTTTGAAAG GGAGTCACAT TTAGATATTT GTAATGTACG TGTAGATGAA 
ATGGAGAAGG AGAAAACCAC ATCTACGTGC TCTCCTAGGA AGACATATGA CAAGGAGAAA 
CATGGAATTG GAAGTGTAAC ATCTATAGAC GAACCTTCAC AGGTCATTAC AGGCAATCAA 
AAAGCTACGT CAAAACTGGA TTTACATTTG GGAGTGTTAC CAACAGACAG AGCAATACTC 
TCGGCAAACG CAGATCTTGA GCTGCTTCAA GAATTATCAA GGAGAACAGA CTTCAATGCT 
GTTCACTCTG CATTTAACTC AGACCCTGGC AGCGCTTCTC AGGACAGCTC TCAAGTTTAC 
AGACGCTGCT CTAAAAAACC AGTACCTTCT TATGGTGAAC AGAAAGCAGT TATTAACACA 
ACACTCCAGT CTGTTCCTAC GAAATCAGAA TATAATTGTC ACCCAGAAAG TGAGATCTTC 
GGTCATGCCA CGTCTAAACC GGAAGTTGTT TTCAAAAGTT CAGAAATAAT AAAATCAGGT 
AGTGGAGGAG AGCAAGGAGT GGGTCCGATT CTCCAACAAA AAGAAGGTAG TTTGGAAAAA 
TCCCAAGACC CGATGATTTC AATCAACGAG CCTCTTGAGA ATCTGGATGA AGTACGTTCT 
GAAAATGAAG GGTTAATGCG TTCTGGTCAG TTACAGTGTT ATTTGGGTGA CAAGGGATCA 
GTGAGTTCTG CTTCTGCTAC TGTCCCTACC CAGGAACTCC AAGCTCAAGG CAGCGAGTCT 
CTGCTTTCAA TATCCATCAA CTCTAAAATA CCTTATTTCC TTTTGTTTCT AATATTTCTT 
GCAACTGTCT ACTACTATGA CCTAATGATA GGCTTGGCAT TCTATCTTTT TTCCTTGTAC 
TGGTTGTACT GGGAAGGGGG GAGGCAAAGA GAGTCTGTCA AGAAAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r3a ( XM_008762717.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760939.1 Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa49298
Clone ID Related Accession (Same CDS sequence)	XM_008762717.2
Accession Version	XM_008762717.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG CCGAAGTACC CGGTCAGATT AGCAAAGATA ACTTTTTAGA AGTTCCTAAT 
CTGTCCGATT CTGTGTGTGA AGATGAAGAA GTTAAAGCGA CCTTCAAGCC TGGCTTCTCC 
CCTCAGCCAA GCAGACGGGG GTCAGAATCT TCTGAAGACT TATATCTGGA CACCCCAACC 
TCAGCTTCCC GGAGAGTTTC CTTTGCTGAC AGCCTTGGAT TCAGTCTTGT GTCTGTTAAA 
GAATTTGATT GTTGGGACTT ACCAAGTGTT TCAACTGATT TTGACTTAAC TAGGGATGTC 
CTCCACACAG ATGAATATAT TTTATCTCCA CTGTTTGACT TGCCTTCTTC CAAAGAAGAG 
CTTATGGAAC AACTTCAAGT CCAGAAGGCA GTGCTGGAGT CGGCTGAACA CCTTCCTGGG 
TCGACAAGTG TGAAGGGCAT CATTCGAGTT CTGAACATTT CTTTTGAGAA GTTAGTGTAT 
GTGCGCATGT CTTTGGACGA CTGGCAGACA CATTATGACA TTTTAGCAGA ATATGTTCCT 
AATTCGTGCG ATGGTGAAAC TGACCAGTTC TCCTTTAAGA TTTCATTGGT TCCACCTTAT 
CAAATGGACG GCGGTAAAGT GGAGTTTTGC ATACGCTATG AAACTTCTGT TGGTACATTT 
TGGTCAAATA ATAATGGGAC AAATTATATA TTGGTTTGTC AAAAGAAAAC AAAGGAGCCA 
GAGCCTGTAG TAAAGCCACT GGAAGAAGGT CCTAGTAGAC AAATAAAAGG CTGCTTAAAG 
GTAAAATCAA GTAAAGAAGA ACCATTGCTA ACACCAGAAG AAAATAAGCT TGGGACTTTG 
AAGTTCACAG AGTCCTACAT CCCAACCATT ATTTGTTCTC ATGAGGACAG GGATGACTTG 
GGAGCCAATT ATCAACATGT AAAAGACATC GACAAGAAGC ATGATGAACA TAATGAAAAG 
GAATTAGACT TGATGATAAA CCAACGGTTA ATAACTTCCC ATGACGAAAA GAATACATTT 
TCAACAGATG CAGTCAACTC CACAAATAAA GCTGAGGGGT CAGAGAAGAA ACAAGCTCGC 
CATGAAATCC ACACTGACTT GTGCAGAGGG CCTTTGTCTC CGAGTTTATC AGCAGAAAGT 
TCTTTAAAGA GAGATTTCTA CCACAGCAGA AATTATTCCC CAGGAAATGA GCATGGTCAT 
CCACCTTCAG AGGAAATTAT TTCGGATGTG GGAGCGATAG GGTCACCACT GGGAGATACT 
AGTTCTGATG AGTTAATGCA ACTGAAAGTC TGCAGCAAAG AAGACGTAGA CGATAATGCC 
AACCCAGCCC ACGGGAGTGG CAGATTGTGT TCTTCCTCTG ATCAACTAAG GGCAGGTGGT 
CTTGAGAATA ATGAAGCAGG AATCAAGAAA ACTGGAATAA AAGATTACAA ATATTCACAC 
GGAGATTCTT ATTTAGAGGC ATCCACAAGT TTGAGAGAAT CAAATGCGTC TTACAAAGAT 
GAGTACGACA AAGTGGATGA TAAAAGGGAG AAGAAAACAT GCTTAGGTGT TAATGAAAAC 
CCAGGCAAGA ATTTTCAATC AATATTCCAG AACCAAGAGA GACAAATGGG GTGTGCAAAA 
ATAAGCGGTG AAGGGGCTAA TGCTAATAAC CAAGACTTGA CCACTCTACT GAGCAAAGAT 
ACCACAACTA ACACCTGGGC GGTAACCGTC GATCCCTCTC CTTCAACAAA TACAAACGTG 
AATTGGAGAG AAGCCGGAAA TGGTACCGAT TTAGAACGAA GAACTACTGA TCTTGGTTCT 
CCAAGGAATT TCAGTCTCCT GACTGATGGT TATCTTTTTC AAGCTGACAG GGAAAAATCC 
GACTCTAGTA ATTCTGAAGA TCGGAATATG AACCCACAGC ACAAAAAAAA TTGGAACGTT 
CTGGAAAGCC AGCCAGAAAC AAGAGAGACT GAAACAAATA TAACCAAGCA TACCAAAGAA 
CAGGCAGAGT ACAAAGACAC GTGGGGAAAA AGAGATAACA GTAGGAATCT GAAAGCTACT 
TCTACAGAGC AATTGTTTAC GTGCCAAGAA ACAGAATGCT GTGAACTGTC TTCTCTAGCT 
GATCATGGTA TCACTGAGAA AGCACAAGCA GTTGCAGCTT ATATAATTAA GACAACATTA 
GAAAGTACTC CAGAAAGCGC GTCTGCTAGA GGAAAAGCAA TAATTGCTAA GCTACCTCAA 
GAGACAGCAC GACATGACAG GCCCATCGAG GTAAAAGAAA CAGCGTTTGA TCCACATGAA 
GGGCGGAAGG ATGATTCACA TTATTCCCTT TGTCATGGAG ATACAGCAGG TGTAATCCAT 
GACAATGATT TTGAAAGGGA GTCACATTTA GATATTTGTA ATGTACGTGT AGATGAAATG 
GAGAAGGAGA AAACCACATC TACGTGCTCT CCTAGGAAGA CATATGACAA GGAGAAACAT 
GGAATTGGAA GTGTAACATC TATAGACGAA CCTTCACAGG TCATTACAGG CAATCAAAAA 
GCTACGTCAA AACTGGATTT ACATTTGGGA GTGTTACCAA CAGACAGAGC AATACTCTCG 
GCAAACGCAG ATCTTGAGCT GCTTCAAGAA TTATCAAGGA GAACAGACTT CAATGCTGTT 
CACTCTGCAT TTAACTCAGA CCCTGGCAGC GCTTCTCAGG ACAGCTCTCA AGTTTACAGA 
CGCTGCTCTA AAAAACCAGT ACCTTCTTAT GGTGAACAGA AAGCAGTTAT TAACACAACA 
CTCCAGTCTG TTCCTACGAA ATCAGAATAT AATTGTCACC CAGAAAGTGA GATCTTCGGT 
CATGCCACGT CTAAACCGGA AGTTGTTTTC AAAAGTTCAG AAATAATAAA ATCAGGTAGT 
GGAGGAGAGC AAGGAGTGGG TCCGATTCTC CAACAAAAAG AAGGTAGTTT GGAAAAATCC 
CAAGACCCGA TGATTTCAAT CAACGAGCCT CTTGAGAATC TGGATGAAGT ACGTTCTGAA 
AATGAAGGGT TAATGCGTTC TGGTCAGTTA CAGTGTTATT TGGGTGACAA GGGATCAGTG 
AGTTCTGCTT CTGCTACTGT CCCTACCCAG GAACTCCAAG CTCAAGGCAG CGAGTCTCTG 
CTTTCAATAT CCATCAACTC TAAAATACCT TATTTCCTTT TGTTTCTAAT ATTTCTTGCA 
ACTGTCTACT ACTATGACCT AATGATAGGC TTGGCATTCT ATCTTTTTTC CTTGTACTGG 
TTGTACTGGG AAGGGGGGAG GCAAAGAGAG TCTGTCAAGA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760939.1
CDS	105..3392
Translation	MEPAEVPGQISKDNFLEVPNLSDSVCEDEEVKATFKPGFSPQPSRRGSESSEDLYLDTPTSASRRVSFADSLGFSLVSVKEFDCWDLPSVSTDFDLTRDVLHTDEYILSPLFDLPSSKEELMEQLQVQKAVLESAEHLPGSTSVKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQMDGGKVEFCIRYETSVGTFWSNNNGTNYILVCQKKTKEPEPVVKPLEEGPSRQIKGCLKVKSSKEEPLLTPEENKLGTLKFTESYIPTIICSHEDRDDLGANYQHVKDIDKKHDEHNEKELDLMINQRLITSHDEKNTFSTDAVNSTNKAEGSEKKQARHEIHTDLCRGPLSPSLSAESSLKRDFYHSRNYSPGNEHGHPPSEEIISDVGAIGSPLGDTSSDELMQLKVCSKEDVDDNANPAHGSGRLCSSSDQLRAGGLENNEAGIKKTGIKDYKYSHGDSYLEASTSLRESNASYKDEYDKVDDKREKKTCLGVNENPGKNFQSIFQNQERQMGCAKISGEGANANNQDLTTLLSKDTTTNTWAVTVDPSPSTNTNVNWREAGNGTDLERRTTDLGSPRNFSLLTDGYLFQADREKSDSSNSEDRNMNPQHKKNWNVLESQPETRETETNITKHTKEQAEYKDTWGKRDNSRNLKATSTEQLFTCQETECCELSSLADHGITEKAQAVAAYIIKTTLESTPESASARGKAIIAKLPQETARHDRPIEVKETAFDPHEGRKDDSHYSLCHGDTAGVIHDNDFERESHLDICNVRVDEMEKEKTTSTCSPRKTYDKEKHGIGSVTSIDEPSQVITGNQKATSKLDLHLGVLPTDRAILSANADLELLQELSRRTDFNAVHSAFNSDPGSASQDSSQVYRRCSKKPVPSYGEQKAVINTTLQSVPTKSEYNCHPESEIFGHATSKPEVVFKSSEIIKSGSGGEQGVGPILQQKEGSLEKSQDPMISINEPLENLDEVRSENEGLMRSGQLQCYLGDKGSVSSASATVPTQELQAQGSESLLSISINSKIPYFLLFLIFLATVYYYDLMIGLAFYLFSLYWLYWEGGRQRESVKKK

Target ORF information:

RefSeq Version	XM_008762717.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory subunit 3A (Ppp1r3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762717.2

ORF Insert Sequence:

ATGGAGCCCG CCGAAGTACC CGGTCAGATT AGCAAAGATA ACTTTTTAGA AGTTCCTAAT 
CTGTCCGATT CTGTGTGTGA AGATGAAGAA GTTAAAGCGA CCTTCAAGCC TGGCTTCTCC 
CCTCAGCCAA GCAGACGGGG GTCAGAATCT TCTGAAGACT TATATCTGGA CACCCCAACC 
TCAGCTTCCC GGAGAGTTTC CTTTGCTGAC AGCCTTGGAT TCAGTCTTGT GTCTGTTAAA 
GAATTTGATT GTTGGGACTT ACCAAGTGTT TCAACTGATT TTGACTTAAC TAGGGATGTC 
CTCCACACAG ATGAATATAT TTTATCTCCA CTGTTTGACT TGCCTTCTTC CAAAGAAGAG 
CTTATGGAAC AACTTCAAGT CCAGAAGGCA GTGCTGGAGT CGGCTGAACA CCTTCCTGGG 
TCGACAAGTG TGAAGGGCAT CATTCGAGTT CTGAACATTT CTTTTGAGAA GTTAGTGTAT 
GTGCGCATGT CTTTGGACGA CTGGCAGACA CATTATGACA TTTTAGCAGA ATATGTTCCT 
AATTCGTGCG ATGGTGAAAC TGACCAGTTC TCCTTTAAGA TTTCATTGGT TCCACCTTAT 
CAAATGGACG GCGGTAAAGT GGAGTTTTGC ATACGCTATG AAACTTCTGT TGGTACATTT 
TGGTCAAATA ATAATGGGAC AAATTATATA TTGGTTTGTC AAAAGAAAAC AAAGGAGCCA 
GAGCCTGTAG TAAAGCCACT GGAAGAAGGT CCTAGTAGAC AAATAAAAGG CTGCTTAAAG 
GTAAAATCAA GTAAAGAAGA ACCATTGCTA ACACCAGAAG AAAATAAGCT TGGGACTTTG 
AAGTTCACAG AGTCCTACAT CCCAACCATT ATTTGTTCTC ATGAGGACAG GGATGACTTG 
GGAGCCAATT ATCAACATGT AAAAGACATC GACAAGAAGC ATGATGAACA TAATGAAAAG 
GAATTAGACT TGATGATAAA CCAACGGTTA ATAACTTCCC ATGACGAAAA GAATACATTT 
TCAACAGATG CAGTCAACTC CACAAATAAA GCTGAGGGGT CAGAGAAGAA ACAAGCTCGC 
CATGAAATCC ACACTGACTT GTGCAGAGGG CCTTTGTCTC CGAGTTTATC AGCAGAAAGT 
TCTTTAAAGA GAGATTTCTA CCACAGCAGA AATTATTCCC CAGGAAATGA GCATGGTCAT 
CCACCTTCAG AGGAAATTAT TTCGGATGTG GGAGCGATAG GGTCACCACT GGGAGATACT 
AGTTCTGATG AGTTAATGCA ACTGAAAGTC TGCAGCAAAG AAGACGTAGA CGATAATGCC 
AACCCAGCCC ACGGGAGTGG CAGATTGTGT TCTTCCTCTG ATCAACTAAG GGCAGGTGGT 
CTTGAGAATA ATGAAGCAGG AATCAAGAAA ACTGGAATAA AAGATTACAA ATATTCACAC 
GGAGATTCTT ATTTAGAGGC ATCCACAAGT TTGAGAGAAT CAAATGCGTC TTACAAAGAT 
GAGTACGACA AAGTGGATGA TAAAAGGGAG AAGAAAACAT GCTTAGGTGT TAATGAAAAC 
CCAGGCAAGA ATTTTCAATC AATATTCCAG AACCAAGAGA GACAAATGGG GTGTGCAAAA 
ATAAGCGGTG AAGGGGCTAA TGCTAATAAC CAAGACTTGA CCACTCTACT GAGCAAAGAT 
ACCACAACTA ACACCTGGGC GGTAACCGTC GATCCCTCTC CTTCAACAAA TACAAACGTG 
AATTGGAGAG AAGCCGGAAA TGGTACCGAT TTAGAACGAA GAACTACTGA TCTTGGTTCT 
CCAAGGAATT TCAGTCTCCT GACTGATGGT TATCTTTTTC AAGCTGACAG GGAAAAATCC 
GACTCTAGTA ATTCTGAAGA TCGGAATATG AACCCACAGC ACAAAAAAAA TTGGAACGTT 
CTGGAAAGCC AGCCAGAAAC AAGAGAGACT GAAACAAATA TAACCAAGCA TACCAAAGAA 
CAGGCAGAGT ACAAAGACAC GTGGGGAAAA AGAGATAACA GTAGGAATCT GAAAGCTACT 
TCTACAGAGC AATTGTTTAC GTGCCAAGAA ACAGAATGCT GTGAACTGTC TTCTCTAGCT 
GATCATGGTA TCACTGAGAA AGCACAAGCA GTTGCAGCTT ATATAATTAA GACAACATTA 
GAAAGTACTC CAGAAAGCGC GTCTGCTAGA GGAAAAGCAA TAATTGCTAA GCTACCTCAA 
GAGACAGCAC GACATGACAG GCCCATCGAG GTAAAAGAAA CAGCGTTTGA TCCACATGAA 
GGGCGGAAGG ATGATTCACA TTATTCCCTT TGTCATGGAG ATACAGCAGG TGTAATCCAT 
GACAATGATT TTGAAAGGGA GTCACATTTA GATATTTGTA ATGTACGTGT AGATGAAATG 
GAGAAGGAGA AAACCACATC TACGTGCTCT CCTAGGAAGA CATATGACAA GGAGAAACAT 
GGAATTGGAA GTGTAACATC TATAGACGAA CCTTCACAGG TCATTACAGG CAATCAAAAA 
GCTACGTCAA AACTGGATTT ACATTTGGGA GTGTTACCAA CAGACAGAGC AATACTCTCG 
GCAAACGCAG ATCTTGAGCT GCTTCAAGAA TTATCAAGGA GAACAGACTT CAATGCTGTT 
CACTCTGCAT TTAACTCAGA CCCTGGCAGC GCTTCTCAGG ACAGCTCTCA AGTTTACAGA 
CGCTGCTCTA AAAAACCAGT ACCTTCTTAT GGTGAACAGA AAGCAGTTAT TAACACAACA 
CTCCAGTCTG TTCCTACGAA ATCAGAATAT AATTGTCACC CAGAAAGTGA GATCTTCGGT 
CATGCCACGT CTAAACCGGA AGTTGTTTTC AAAAGTTCAG AAATAATAAA ATCAGGTAGT 
GGAGGAGAGC AAGGAGTGGG TCCGATTCTC CAACAAAAAG AAGGTAGTTT GGAAAAATCC 
CAAGACCCGA TGATTTCAAT CAACGAGCCT CTTGAGAATC TGGATGAAGT ACGTTCTGAA 
AATGAAGGGT TAATGCGTTC TGGTCAGTTA CAGTGTTATT TGGGTGACAA GGGATCAGTG 
AGTTCTGCTT CTGCTACTGT CCCTACCCAG GAACTCCAAG CTCAAGGCAG CGAGTCTCTG 
CTTTCAATAT CCATCAACTC TAAAATACCT TATTTCCTTT TGTTTCTAAT ATTTCTTGCA 
ACTGTCTACT ACTATGACCT AATGATAGGC TTGGCATTCT ATCTTTTTTC CTTGTACTGG 
TTGTACTGGG AAGGGGGGAG GCAAAGAGAG TCTGTCAAGA AAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rg1 prevents myocardial hypoxia/reoxygenation injury by regulating mitochondrial dynamics imbalance via modulation of glutamate dehydrogenase and mitofusin 2.
Mitochondrion267-18(2016 Jan)
Dong G,Chen T,Ren X,Zhang Z,Huang W,Liu L,Luo P,Zhou H

Functional diversity of protein phosphatase-1, a cellular economizer and reset button.
Physiological reviews84(1)1-39(2004 Jan)
Ceulemans H,Bollen M

Male preponderance in early diagnosed type 2 diabetes is associated with the ARE insertion/deletion polymorphism in the PPP1R3A locus.
BMC genetics411(2003 Jun)
Doney AS,Fischer B,Cecil JE,Cohen PT,Boyle DI,Leese G,Morris AD,Palmer CN

Digenic inheritance of severe insulin resistance in a human pedigree.
Nature genetics31(4)379-84(2002 Aug)
Savage DB,Agostini M,Barroso I,Gurnell M,Luan J,Meirhaeghe A,Harding AH,Ihrke G,Rajanayagam O,Soos MA,George S,Berger D,Thomas EL,Bell JD,Meeran K,Ross RJ,Vidal-Puig A,Wareham NJ,O'Rahilly S,Chatterjee VK,Schafer AJ

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Ppp1r3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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