Zfp458 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp458
Entrez Gene ID	499563
Full Name	zinc finger protein 458
General protein information	Preferred Names zinc finger protein 458 Names zinc finger protein 458
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142962.1	NP_001136434.1	zinc finger protein 458 isoform 1
NM_001024299.1	NP_001019470.1	zinc finger protein 458 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zfp458	NP_001001152.1
Homo sapiens (human)	ZNF43	NP_001243582.1
Rattus norvegicus (Norway rat)	Zfp458	NP_001136434.1
	ZNF43	XP_002801204.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Zfp458 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp458 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04354	NM_001142962.1 Latest version!	Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa06252	NM_001024299.1 Latest version!	Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp458 ( NM_001142962.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001136434.1 Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa04354
Clone ID Related Accession (Same CDS sequence)	NM_001142962.1
Accession Version	NM_001142962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-07
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein 458 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07009106.1. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATA TACTGTCATT CAGAGATGTG GCCATTGATT TCTCTGCAGA GGAGTTGGAA 
TACCTTGGTC CTGCTCAGTG GAGTCTGTAC CGGGATGTGA TGTTGGAGAA TTACAGCAAC 
CTTGTGTTCC TGGGTCTTGC TTCCTCTAAG CCATACCTGA TTACACTTCT GGAACAAATA 
AAAGAGCCTT CAGATATGAA GAAACGAGAG GCTATCACTC TGCACCCAGG GGGAAAATGC 
TATATGTGCG AAGAATGTGG CCAAGTCTTT GAGTGGAAAA AAGTACTTCA GAATCACCAG 
AAAATTCATT TAAGAGCAAA GCCTTACAAG TGTGAAGAAT GTGGCAAGTC TTTCCATTTT 
CCATCATTAC TTTCTGCACA CAAAAGAAAT CATACAGGAG AAAAACTCTA CAACTGTGAA 
ATATGTTGTA AAGCCTTCCA TGCTTCTTCA CTGCTTTCTT TACACAAGAG AATTCATACA 
GGACAGAAAC CCCACGAGTC TGAAAATTAT AGCAAGGACT TCCATTATCC ATCATTACTT 
TCTCAAAACA AGGTAGTTCA TACAGGAGAA AAATCCTACC AATGTGAAGA CCATGGCAAG 
ACCTTCCATT ATCCATCAAC ATTTTCCAAG CATAAGAAAA TTCATACAGG AGAGAAACCA 
CACAAGTGTG AAGTATGTGG CAAGGCCTTC GACTATCCAT CAAGACTTTC CAACCATAAG 
AGAATTCATA CAGGAGAAAA ACCCTACAAA TGTGAAGTAT GTGACAGAGC CTTCCATGAT 
CCATCAAAAC TTTCTCAACA CAAAATAATT CATACAGGAG AAAAACCCTA CAAATGTGAC 
GTTTGTGGCA AGGCCTTTCA TTATCCATCC ATACTTTCTA AACATAAGAT AATTCATACA 
GAAGAGAATC TCTGCAAGTG TGATATATGT GGCAAGGCCT TCCATTATCC ATTATTACTT 
TCTCAACACA AAATAGTTCA TACAGGAGAA AAACCCTACA AATGTGAAGA CTGTGGCAAA 
GCCTTCTATT ATCCATCAAG ACTTTCCAGA CATAAGAAAA TACATACAGG AGAGAAACCA 
TACAAGTGTG AAGTATGTGG CAAGGCCTTC CATTATCTAT CCATACTTTC TAAACACAAG 
ATAGTTCATA CAGAAGAGAA TCCCTACAAG TGTGAAGTAT GTGGCAAGGC CTTTGATTAT 
CCATCAAGGC TTTCCAACCA TAAAAAAATT CATACAGAAG AGAAACCATA CAAGTGTGAA 
GTATGTGGAA ATGCCTTCTG TTTTTCATCA TCACTTCGTA AACACAAGAT AATTCACACA 
GGAGAGAAAC CCTACAAGTG TGAAGTATGT GGCAAGGCCT TTGGTTCTCC ATCAAGACTT 
TCCAAACATA AGAAAATTCA TACAGAAGAG AAACCATACA AGTGTGAAGT ATGTGGAAAG 
GCCTTCCACT TTCCATCATT ACTCTCGGTA CACAAGAGAA TTCATACGGG AGAAAAACCC 
TACAAGTGTG AGATATGTGG CAAAGCCTTC TATTGTGCAT CAACACTTTC TGTACACAAG 
AGAATTCATA CTGGAGAAAA CAACTACAAG TGTGAAGTAT GTGGCAAAGC CTTCCATTGT 
CCATCAACAC TTTCAGTGCA CAGGAGAATT CATACTCAAG AAAAACCATA CAAGTGTGAA 
GTATGTGGCC AGGCATTCCA TGTTTCATCT AAACTCTCTT ATCATAAGAG AATTCATACA 
GCAGAAAAAC CCTACAAATG TGAAATTTGT GGCAAGGCCT TCTATTATCC ATCAAGACTT 
TCCAAGCATA AGATAGTTCA TATGGGAGAG AAACGCTACC ATGGTCAAGC ATGTGGCAAG 
GCCCTTGATT ATCCATCAAG ACATTCTAAA CATAAGAAAC TTAATCCAGG AGAGAAACCA 
CACAAATGTG AAATATGTGG AAAAGCCTTC CACTTTCCAT CATTACTTTC GATACACAAG 
AAAATTCATA CTGGAGAAAA AACCTGTAAG TGTGAAGTAT GTGGCAAGGA CTTCCATTAT 
CCATCAAGAC TTTCAAACCA CAAGAAAATT CATACAGAAG GGAAAGCATA CAAGTGTGAT 
ATTTGTGACA AGACCTTCAA TTATAAATCA TTACTTTCTA AACACAAGAT TGGTCATACA 
GTAGAGAATA CCTACCACAG TGAAGTATGT GGGAAGGCCT TTGGTTATTC CTCAAGAATT 
TCCAAAGATA AGAAACTTCA TTCAGGAGAG AAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136434.1
CDS	33..2291
Translation	MEDILSFRDVAIDFSAEELEYLGPAQWSLYRDVMLENYSNLVFLGLASSKPYLITLLEQIKEPSDMKKREAITLHPGGKCYMCEECGQVFEWKKVLQNHQKIHLRAKPYKCEECGKSFHFPSLLSAHKRNHTGEKLYNCEICCKAFHASSLLSLHKRIHTGQKPHESENYSKDFHYPSLLSQNKVVHTGEKSYQCEDHGKTFHYPSTFSKHKKIHTGEKPHKCEVCGKAFDYPSRLSNHKRIHTGEKPYKCEVCDRAFHDPSKLSQHKIIHTGEKPYKCDVCGKAFHYPSILSKHKIIHTEENLCKCDICGKAFHYPLLLSQHKIVHTGEKPYKCEDCGKAFYYPSRLSRHKKIHTGEKPYKCEVCGKAFHYLSILSKHKIVHTEENPYKCEVCGKAFDYPSRLSNHKKIHTEEKPYKCEVCGNAFCFSSSLRKHKIIHTGEKPYKCEVCGKAFGSPSRLSKHKKIHTEEKPYKCEVCGKAFHFPSLLSVHKRIHTGEKPYKCEICGKAFYCASTLSVHKRIHTGENNYKCEVCGKAFHCPSTLSVHRRIHTQEKPYKCEVCGQAFHVSSKLSYHKRIHTAEKPYKCEICGKAFYYPSRLSKHKIVHMGEKRYHGQACGKALDYPSRHSKHKKLNPGEKPHKCEICGKAFHFPSLLSIHKKIHTGEKTCKCEVCGKDFHYPSRLSNHKKIHTEGKAYKCDICDKTFNYKSLLSKHKIGHTVENTYHSEVCGKAFGYSSRISKDKKLHSGEKL

Target ORF information:

RefSeq Version	NM_001142962.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142962.1

ORF Insert Sequence:

ATGGAGGATA TACTGTCATT CAGAGATGTG GCCATTGATT TCTCTGCAGA GGAGTTGGAA 
TACCTTGGTC CTGCTCAGTG GAGTCTGTAC CGGGATGTGA TGTTGGAGAA TTACAGCAAC 
CTTGTGTTCC TGGGTCTTGC TTCCTCTAAG CCATACCTGA TTACACTTCT GGAACAAATA 
AAAGAGCCTT CAGATATGAA GAAACGAGAG GCTATCACTC TGCACCCAGG GGGAAAATGC 
TATATGTGCG AAGAATGTGG CCAAGTCTTT GAGTGGAAAA AAGTACTTCA GAATCACCAG 
AAAATTCATT TAAGAGCAAA GCCTTACAAG TGTGAAGAAT GTGGCAAGTC TTTCCATTTT 
CCATCATTAC TTTCTGCACA CAAAAGAAAT CATACAGGAG AAAAACTCTA CAACTGTGAA 
ATATGTTGTA AAGCCTTCCA TGCTTCTTCA CTGCTTTCTT TACACAAGAG AATTCATACA 
GGACAGAAAC CCCACGAGTC TGAAAATTAT AGCAAGGACT TCCATTATCC ATCATTACTT 
TCTCAAAACA AGGTAGTTCA TACAGGAGAA AAATCCTACC AATGTGAAGA CCATGGCAAG 
ACCTTCCATT ATCCATCAAC ATTTTCCAAG CATAAGAAAA TTCATACAGG AGAGAAACCA 
CACAAGTGTG AAGTATGTGG CAAGGCCTTC GACTATCCAT CAAGACTTTC CAACCATAAG 
AGAATTCATA CAGGAGAAAA ACCCTACAAA TGTGAAGTAT GTGACAGAGC CTTCCATGAT 
CCATCAAAAC TTTCTCAACA CAAAATAATT CATACAGGAG AAAAACCCTA CAAATGTGAC 
GTTTGTGGCA AGGCCTTTCA TTATCCATCC ATACTTTCTA AACATAAGAT AATTCATACA 
GAAGAGAATC TCTGCAAGTG TGATATATGT GGCAAGGCCT TCCATTATCC ATTATTACTT 
TCTCAACACA AAATAGTTCA TACAGGAGAA AAACCCTACA AATGTGAAGA CTGTGGCAAA 
GCCTTCTATT ATCCATCAAG ACTTTCCAGA CATAAGAAAA TACATACAGG AGAGAAACCA 
TACAAGTGTG AAGTATGTGG CAAGGCCTTC CATTATCTAT CCATACTTTC TAAACACAAG 
ATAGTTCATA CAGAAGAGAA TCCCTACAAG TGTGAAGTAT GTGGCAAGGC CTTTGATTAT 
CCATCAAGGC TTTCCAACCA TAAAAAAATT CATACAGAAG AGAAACCATA CAAGTGTGAA 
GTATGTGGAA ATGCCTTCTG TTTTTCATCA TCACTTCGTA AACACAAGAT AATTCACACA 
GGAGAGAAAC CCTACAAGTG TGAAGTATGT GGCAAGGCCT TTGGTTCTCC ATCAAGACTT 
TCCAAACATA AGAAAATTCA TACAGAAGAG AAACCATACA AGTGTGAAGT ATGTGGAAAG 
GCCTTCCACT TTCCATCATT ACTCTCGGTA CACAAGAGAA TTCATACGGG AGAAAAACCC 
TACAAGTGTG AGATATGTGG CAAAGCCTTC TATTGTGCAT CAACACTTTC TGTACACAAG 
AGAATTCATA CTGGAGAAAA CAACTACAAG TGTGAAGTAT GTGGCAAAGC CTTCCATTGT 
CCATCAACAC TTTCAGTGCA CAGGAGAATT CATACTCAAG AAAAACCATA CAAGTGTGAA 
GTATGTGGCC AGGCATTCCA TGTTTCATCT AAACTCTCTT ATCATAAGAG AATTCATACA 
GCAGAAAAAC CCTACAAATG TGAAATTTGT GGCAAGGCCT TCTATTATCC ATCAAGACTT 
TCCAAGCATA AGATAGTTCA TATGGGAGAG AAACGCTACC ATGGTCAAGC ATGTGGCAAG 
GCCCTTGATT ATCCATCAAG ACATTCTAAA CATAAGAAAC TTAATCCAGG AGAGAAACCA 
CACAAATGTG AAATATGTGG AAAAGCCTTC CACTTTCCAT CATTACTTTC GATACACAAG 
AAAATTCATA CTGGAGAAAA AACCTGTAAG TGTGAAGTAT GTGGCAAGGA CTTCCATTAT 
CCATCAAGAC TTTCAAACCA CAAGAAAATT CATACAGAAG GGAAAGCATA CAAGTGTGAT 
ATTTGTGACA AGACCTTCAA TTATAAATCA TTACTTTCTA AACACAAGAT TGGTCATACA 
GTAGAGAATA CCTACCACAG TGAAGTATGT GGGAAGGCCT TTGGTTATTC CTCAAGAATT 
TCCAAAGATA AGAAACTTCA TTCAGGAGAG AAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp458 ( NM_001024299.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019470.1 Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa06252
Clone ID Related Accession (Same CDS sequence)	NM_001024299.1
Accession Version	NM_001024299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-07
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein 458 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085784.1. On May 27, 2005 this sequence version replaced XM_574887.1. Transcript Variant: This variant (2) uses an alternate splice pattern in the 5' region, compared to variant 1. The resulting isoform (2) is translated from a downstream AUG and has a shorter N-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC085784.1, CK480999.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689604 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGGAGA ATTACAGCAA CCTTGTGTTC CTGGGTCTTG CTTCCTCTAA GCCATACCTG 
ATTACACTTC TGGAACAAAT AAAAGAGCCT TCAGATATGA AGAAACGAGA GGCTATCACT 
CTGCACCCAG GGGGAAAATG CTATATGTGC GAAGAATGTG GCCAAGTCTT TGAGTGGAAA 
AAAGTACTTC AGAATCACCA GAAAATTCAT TTAAGAGCAA AGCCTTACAA GTGTGAAGAA 
TGTGGCAAGT CTTTCCATTT TCCATCATTA CTTTCTGCAC ACAAAAGAAA TCATACAGGA 
GAAAAACTCT ACAACTGTGA AATATGTTGT AAAGCCTTCC ATGCTTCTTC ACTGCTTTCT 
TTACACAAGA GAATTCATAC AGGACAGAAA CCCCACGAGT CTGAAAATTA TAGCAAGGAC 
TTCCATTATC CATCATTACT TTCTCAAAAC AAGGTAGTTC ATACAGGAGA AAAATCCTAC 
CAATGTGAAG ACCATGGCAA GACCTTCCAT TATCCATCAA CATTTTCCAA GCATAAGAAA 
ATTCATACAG GAGAGAAACC ACACAAGTGT GAAGTATGTG GCAAGGCCTT CGACTATCCA 
TCAAGACTTT CCAACCATAA GAGAATTCAT ACAGGAGAAA AACCCTACAA ATGTGAAGTA 
TGTGACAGAG CCTTCCATGA TCCATCAAAA CTTTCTCAAC ACAAAATAAT TCATACAGGA 
GAAAAACCCT ACAAATGTGA CGTTTGTGGC AAGGCCTTTC ATTATCCATC CATACTTTCT 
AAACATAAGA TAATTCATAC AGAAGAGAAT CTCTGCAAGT GTGATATATG TGGCAAGGCC 
TTCCATTATC CATTATTACT TTCTCAACAC AAAATAGTTC ATACAGGAGA AAAACCCTAC 
AAATGTGAAG ACTGTGGCAA AGCCTTCTAT TATCCATCAA GACTTTCCAG ACATAAGAAA 
ATACATACAG GAGAGAAACC ATACAAGTGT GAAGTATGTG GCAAGGCCTT CCATTATCTA 
TCCATACTTT CTAAACACAA GATAGTTCAT ACAGAAGAGA ATCCCTACAA GTGTGAAGTA 
TGTGGCAAGG CCTTTGATTA TCCATCAAGG CTTTCCAACC ATAAAAAAAT TCATACAGAA 
GAGAAACCAT ACAAGTGTGA AGTATGTGGA AATGCCTTCT GTTTTTCATC ATCACTTCGT 
AAACACAAGA TAATTCACAC AGGAGAGAAA CCCTACAAGT GTGAAGTATG TGGCAAGGCC 
TTTGGTTCTC CATCAAGACT TTCCAAACAT AAGAAAATTC ATACAGAAGA GAAACCATAC 
AAGTGTGAAG TATGTGGAAA GGCCTTCCAC TTTCCATCAT TACTCTCGGT ACACAAGAGA 
ATTCATACGG GAGAAAAACC CTACAAGTGT GAGATATGTG GCAAAGCCTT CTATTGTGCA 
TCAACACTTT CTGTACACAA GAGAATTCAT ACTGGAGAAA ACAACTACAA GTGTGAAGTA 
TGTGGCAAAG CCTTCCATTG TCCATCAACA CTTTCAGTGC ACAGGAGAAT TCATACTCAA 
GAAAAACCAT ACAAGTGTGA AGTATGTGGC CAGGCATTCC ATGTTTCATC TAAACTCTCT 
TATCATAAGA GAATTCATAC AGCAGAAAAA CCCTACAAAT GTGAAATTTG TGGCAAGGCC 
TTCTATTATC CATCAAGACT TTCCAAGCAT AAGATAGTTC ATATGGGAGA GAAACGCTAC 
CATGGTCAAG CATGTGGCAA GGCCCTTGAT TATCCATCAA GACATTCTAA ACATAAGAAA 
CTTAATCCAG GAGAGAAACC ACACAAATGT GAAATATGTG GAAAAGCCTT CCACTTTCCA 
TCATTACTTT CGATACACAA GAAAATTCAT ACTGGAGAAA AAACCTGTAA GTGTGAAGTA 
TGTGGCAAGG ACTTCCATTA TCCATCAAGA CTTTCAAACC ACAAGAAAAT TCATACAGAA 
GGGAAAGCAT ACAAGTGTGA TATTTGTGAC AAGACCTTCA ATTATAAATC ATTACTTTCT 
AAACACAAGA TTGGTCATAC AGTAGAGAAT ACCTACCACA GTGAAGTATG TGGGAAGGCC 
TTTGGTTATT CCTCAAGAAT TTCCAAAGAT AAGAAACTTC ATTCAGGAGA GAAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019470.1
CDS	231..2390
Translation	MLENYSNLVFLGLASSKPYLITLLEQIKEPSDMKKREAITLHPGGKCYMCEECGQVFEWKKVLQNHQKIHLRAKPYKCEECGKSFHFPSLLSAHKRNHTGEKLYNCEICCKAFHASSLLSLHKRIHTGQKPHESENYSKDFHYPSLLSQNKVVHTGEKSYQCEDHGKTFHYPSTFSKHKKIHTGEKPHKCEVCGKAFDYPSRLSNHKRIHTGEKPYKCEVCDRAFHDPSKLSQHKIIHTGEKPYKCDVCGKAFHYPSILSKHKIIHTEENLCKCDICGKAFHYPLLLSQHKIVHTGEKPYKCEDCGKAFYYPSRLSRHKKIHTGEKPYKCEVCGKAFHYLSILSKHKIVHTEENPYKCEVCGKAFDYPSRLSNHKKIHTEEKPYKCEVCGNAFCFSSSLRKHKIIHTGEKPYKCEVCGKAFGSPSRLSKHKKIHTEEKPYKCEVCGKAFHFPSLLSVHKRIHTGEKPYKCEICGKAFYCASTLSVHKRIHTGENNYKCEVCGKAFHCPSTLSVHRRIHTQEKPYKCEVCGQAFHVSSKLSYHKRIHTAEKPYKCEICGKAFYYPSRLSKHKIVHMGEKRYHGQACGKALDYPSRHSKHKKLNPGEKPHKCEICGKAFHFPSLLSIHKKIHTGEKTCKCEVCGKDFHYPSRLSNHKKIHTEGKAYKCDICDKTFNYKSLLSKHKIGHTVENTYHSEVCGKAFGYSSRISKDKKLHSGEKL

Target ORF information:

RefSeq Version	NM_001024299.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger protein 458 (Zfp458), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024299.1

ORF Insert Sequence:

ATGTTGGAGA ATTACAGCAA CCTTGTGTTC CTGGGTCTTG CTTCCTCTAA GCCATACCTG 
ATTACACTTC TGGAACAAAT AAAAGAGCCT TCAGATATGA AGAAACGAGA GGCTATCACT 
CTGCACCCAG GGGGAAAATG CTATATGTGC GAAGAATGTG GCCAAGTCTT TGAGTGGAAA 
AAAGTACTTC AGAATCACCA GAAAATTCAT TTAAGAGCAA AGCCTTACAA GTGTGAAGAA 
TGTGGCAAGT CTTTCCATTT TCCATCATTA CTTTCTGCAC ACAAAAGAAA TCATACAGGA 
GAAAAACTCT ACAACTGTGA AATATGTTGT AAAGCCTTCC ATGCTTCTTC ACTGCTTTCT 
TTACACAAGA GAATTCATAC AGGACAGAAA CCCCACGAGT CTGAAAATTA TAGCAAGGAC 
TTCCATTATC CATCATTACT TTCTCAAAAC AAGGTAGTTC ATACAGGAGA AAAATCCTAC 
CAATGTGAAG ACCATGGCAA GACCTTCCAT TATCCATCAA CATTTTCCAA GCATAAGAAA 
ATTCATACAG GAGAGAAACC ACACAAGTGT GAAGTATGTG GCAAGGCCTT CGACTATCCA 
TCAAGACTTT CCAACCATAA GAGAATTCAT ACAGGAGAAA AACCCTACAA ATGTGAAGTA 
TGTGACAGAG CCTTCCATGA TCCATCAAAA CTTTCTCAAC ACAAAATAAT TCATACAGGA 
GAAAAACCCT ACAAATGTGA CGTTTGTGGC AAGGCCTTTC ATTATCCATC CATACTTTCT 
AAACATAAGA TAATTCATAC AGAAGAGAAT CTCTGCAAGT GTGATATATG TGGCAAGGCC 
TTCCATTATC CATTATTACT TTCTCAACAC AAAATAGTTC ATACAGGAGA AAAACCCTAC 
AAATGTGAAG ACTGTGGCAA AGCCTTCTAT TATCCATCAA GACTTTCCAG ACATAAGAAA 
ATACATACAG GAGAGAAACC ATACAAGTGT GAAGTATGTG GCAAGGCCTT CCATTATCTA 
TCCATACTTT CTAAACACAA GATAGTTCAT ACAGAAGAGA ATCCCTACAA GTGTGAAGTA 
TGTGGCAAGG CCTTTGATTA TCCATCAAGG CTTTCCAACC ATAAAAAAAT TCATACAGAA 
GAGAAACCAT ACAAGTGTGA AGTATGTGGA AATGCCTTCT GTTTTTCATC ATCACTTCGT 
AAACACAAGA TAATTCACAC AGGAGAGAAA CCCTACAAGT GTGAAGTATG TGGCAAGGCC 
TTTGGTTCTC CATCAAGACT TTCCAAACAT AAGAAAATTC ATACAGAAGA GAAACCATAC 
AAGTGTGAAG TATGTGGAAA GGCCTTCCAC TTTCCATCAT TACTCTCGGT ACACAAGAGA 
ATTCATACGG GAGAAAAACC CTACAAGTGT GAGATATGTG GCAAAGCCTT CTATTGTGCA 
TCAACACTTT CTGTACACAA GAGAATTCAT ACTGGAGAAA ACAACTACAA GTGTGAAGTA 
TGTGGCAAAG CCTTCCATTG TCCATCAACA CTTTCAGTGC ACAGGAGAAT TCATACTCAA 
GAAAAACCAT ACAAGTGTGA AGTATGTGGC CAGGCATTCC ATGTTTCATC TAAACTCTCT 
TATCATAAGA GAATTCATAC AGCAGAAAAA CCCTACAAAT GTGAAATTTG TGGCAAGGCC 
TTCTATTATC CATCAAGACT TTCCAAGCAT AAGATAGTTC ATATGGGAGA GAAACGCTAC 
CATGGTCAAG CATGTGGCAA GGCCCTTGAT TATCCATCAA GACATTCTAA ACATAAGAAA 
CTTAATCCAG GAGAGAAACC ACACAAATGT GAAATATGTG GAAAAGCCTT CCACTTTCCA 
TCATTACTTT CGATACACAA GAAAATTCAT ACTGGAGAAA AAACCTGTAA GTGTGAAGTA 
TGTGGCAAGG ACTTCCATTA TCCATCAAGA CTTTCAAACC ACAAGAAAAT TCATACAGAA 
GGGAAAGCAT ACAAGTGTGA TATTTGTGAC AAGACCTTCA ATTATAAATC ATTACTTTCT 
AAACACAAGA TTGGTCATAC AGTAGAGAAT ACCTACCACA GTGAAGTATG TGGGAAGGCC 
TTTGGTTATT CCTCAAGAAT TTCCAAAGAT AAGAAACTTC ATTCAGGAGA GAAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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Zfp458 cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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ORF Insert Sequence:

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