Dock8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dock8
Entrez Gene ID	499337
Full Name	dedicator of cytokinesis 8
General protein information	Preferred Names dedicator of cytokinesis 8 Names dedicator of cytokinesis protein 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q51

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231245.2	XP_006231307.1	dedicator of cytokinesis protein 8 isoform X1
XM_006231244.3	XP_006231306.1	dedicator of cytokinesis protein 8 isoform X1
NM_001037793.2	NP_001032882.2	dedicator of cytokinesis protein 8
XM_008760310.2	XP_008758532.1	dedicator of cytokinesis protein 8 isoform X1
XM_008760311.2	XP_008758533.1	dedicator of cytokinesis protein 8 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOCK8	NP_001178461.1
Danio rerio (zebrafish)	dock8	XP_001920107.4
Homo sapiens (human)	DOCK8	NP_982272.2
Pan troglodytes (chimpanzee)	DOCK8	XP_003312029.1
Xenopus tropicalis (tropical clawed frog)	dock8	XP_002935647.2
Canis lupus familiaris (dog)	DOCK8	XP_005615946.1
Gallus gallus (chicken)	DOCK8	XP_424926.4
	LOC698081	XP_002808151.1
Rattus norvegicus (Norway rat)	Dock8	NP_001032882.2
Mus musculus (house mouse)	Dock8	NP_083061.2

Gene Ontology
Bibliography

Related articles in PubMed

LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis.
Xu X, Han L, Zhao G, Xue S, Gao Y, Xiao J, Zhang S, Chen P, Wu ZY, Ding J, Hu R, Wei B, Wang H
The Journal of experimental medicine214(1)209-226(2017 Jan)

A novel Dock8 gene mutation confers diabetogenic susceptibility in the LEW.1AR1/Ztm-iddm rat, an animal model of human type 1 diabetes.
Arndt T, Wedekind D, J?rns A, Tsiavaliaris G, Cuppen E, Hedrich HJ, Lenzen S
Diabetologia58(12)2800-9(2015 Dec)

Coincidental loss of DOCK8 function in NLRP10-deficient and C3H/HeJ mice results in defective dendritic cell migration.
Krishnaswamy JK, Singh A, Gowthaman U, Wu R, Gorrepati P, Sales Nascimento M, Gallman A, Liu D, Rhebergen AM, Calabro S, Xu L, Ranney P, Srivastava A, Ranson M, Gorham JD, McCaw Z, Kleeberger SR, Heinz LX, M?ller AC, Bennett KL, Superti-Furga G, Henao-Mejia J, Sutterwala FS, Williams A, Flavell RA, Eisenbarth SC
Proceedings of the National Academy of Sciences of the United States of America112(10)3056-61(2015 Mar)

DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses.
Harada Y, Tanaka Y, Terasawa M, Pieczyk M, Habiro K, Katakai T, Hanawa-Suetsugu K, Kukimoto-Niino M, Nishizaki T, Shirouzu M, Duan X, Uruno T, Nishikimi A, Sanematsu F, Yokoyama S, Stein JV, Kinashi T, Fukui Y
Blood119(19)4451-61(2012 May)

DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.
Randall KL, Chan SS, Ma CS, Fung I, Mei Y, Yabas M, Tan A, Arkwright PD, Al Suwairi W, Lugo Reyes SO, Yamazaki-Nakashimada MA, Garcia-Cruz Mde L, Smart JM, Picard C, Okada S, Jouanguy E, Casanova JL, Lambe T, Cornall RJ, Russell S, Oliaro J, Tangye SG, Bertram EM, Goodnow CC
The Journal of experimental medicine208(11)2305-20(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Dock8 mutation gave rise to a new type 1 diabetes rat model with very close similarity to type 1 diabetes in humans
Title: A novel Dock8 gene mutation confers diabetogenic susceptibility in the LEW.1AR1/Ztm-iddm rat, an animal model of human type 1 diabetes.

The following Dock8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dock8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08294	NM_001037793.2 Latest version!	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24021	XM_008760310.2 Latest version!	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24021	XM_006231245.2 Latest version!	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24021	XM_006231244.3 Latest version!	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa24021	XM_008760311.2 Latest version!	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dock8 ( NM_001037793.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001032882.2 Rattus norvegicus dedicator of cytokinesis 8 (Dock8), mRNA.

Price & Availability↑

CloneID	ORa08294
Clone ID Related Accession (Same CDS sequence)	NM_001037793.2
Accession Version	NM_001037793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-04
Organism	Rattus norvegicus(Norway rat)
Product	dedicator of cytokinesis protein 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07006624.1, AABR07006625.1, AABR07006626.1 and AABR07006627.1. On Sep 4, 2008 this sequence version replaced NM_001037793.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689597 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACGC TACCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCATCAGAAA TAAGGAAGCA GTTTACGCTC CCGCCCAACC TCGGACAGTA CCATCGGCAC 
AGCCTCAGTA CCTCTGGTTT CCCTTCTCTT CAGCTACCTC AGCTGTATGA GCCCGTTGAG 
CCTGTGGACT TTGAAGGACT TGTGATGACG CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAGGAGTGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGGGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTGAACC GGAAAAACCA AGGAAGTCCA 
GAGTTTTGCA GCTTCAGAAA GACTGGATCT CGCAGAGATT TTCACAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG CCACTCAGAC GGGACCCCGC 
CATCGCTTCA ACGTGCTGTG TGATGTGTCT GGGAAGGGCC CCATCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGACGAGCGG CTGGAAAACC TGCTTCAGCT GAGTGCTGAG 
GACTTTGAGA AGGAGAAAGA GGAGGCCCGA AAGACCAATC GGACGGCTGA GCTCTTCGCC 
CTCTATCCAT CCGTAGATGA GGAGGATGCT GTGGAAATCC GTCCAGTACC AGAATGTCCT 
AAGGAACATC TGGGCAACAG AATATTGGTG AAGGTGCTGA CCCTGAAGTT TGAGATTGAA 
ATTGAACCTC TGTTTGCCAG TATTGCCCTC TATGATGTTA AAGAAAGGAA AAAGATCTCA 
GAAAACTTCC ACTGTGACCT GAACTCCGAC CAGTTCAAAG GGTTTCTGCG AGCTCACACA 
CCCTCGGTTG ACCCGTCGAG TCAGGCAAGG TCTGCAGTGT TCTCGGTCAC CTACCCATCT 
TCAGACATCT ACCTGGTTGT CAAGATTGAA AAGGTCCTTC AGCAAGGAGA GATCGCAGAC 
TGTGCAGAAC CCTACATGAT CATCAAAGAA AGCGATGGTG GAAAGAGTAA AGAAAAGGTT 
GAAAAACTAA AACTCCAAGC TGAATCCTTC TGCCAACGTT TGGGGAAATA CCGGATGCCC 
TTCGCCTGGG CCCCCATCAG CTTAGCAAGC TTCTTCAACG TCTCCACCCT TGAAAGGGAG 
AGCACAGACT TGGAGCCTGG GGCTGGGAGA AACTCTGTGG GGGAGAGGAG GAGTTTGTCC 
CAGTCCAGGA GGCCTTCTGA GAGAACCCTC TCCTTTGAGG AAAATGGAGT TGGATCCAAC 
TTCAAAACCA CCACCTTGGC CACTAACATC TTCTTCAAGC AGGAGGGAGA TCGCCTTAGT 
GATGAAGACT TGTTCAAGTT TTTAGCTGAC TACAAGAGAT CTTCGTCCCT ACAGCGAAGA 
GTGAAATCCA TCCCAGGCTC ACTTAGGCTG GAGATAGCCC CAGCTCCTGA CGTGATGAAC 
TGCTGCCTGA CACCCGAGAT GCTGCCGGTC AAACCCTTCC CTGAAAATCG GACGCGTCCC 
CACAAGGAGA TTTTGGAATT TCCAATCCGG GAAGTGTATG TTCCCCACAC CGTGTACAGA 
AACCTTCTGT ACGTATACCC ACAGAGACTG AACTTTGCTA ACAAGCTAGC ATCCGCCCGG 
AACATTACAA TAAAGATTCA GTTTATGTGT GGAGAAGACC CCAGCAATGC TATGCCTGTC 
ATCTTTGGGA AGTCCAGTGG GCCTGAATTT CTGCAGGAAG TGTACACAGC TATTACATAC 
CATAATAAGT CTCCCGACTT TTATGAAGAA GTAAAGATTA AGCTTCCGGC CAAGCTCACA 
GTGAATCACC ACCTCCTCTT CACCTTCTAC CACATCAGCT GTCAGCAGAA GCAAGGCGCC 
TCTGGAGAAA GCCTTCTGGG ATACTCGTGG CTGCCGATTC TCCTGAACGA ACGTCTTCAA 
ACCGGATCCT ACTGTCTACC AGTTGCCTTG GAAAAACTAC CACCTAACTA CTCCATACAT 
TCTGCTGAGA AAGTCCCTTT ACAGAATCCT CCCATTAAGT GGGCCGAGGG CCATAAGGGG 
GTATTTAATA TTGAAGTGCA AGCTGTTTCC TCCGTTCACA CCCAGGATAA CCACCTGGAG 
AAGTTCTTCA CCCTTTGCCA CTCCCTGGAG AGCCAGGTGA CCTTCCCTAT CCGCGTGCTG 
GACCAGAAGA TCACGGAGAA CACGCTGGAG CATGAGCTTA AGCTCAGCAT CATCTGCCTC 
AACTCCTCCC GCCTGGAGCC CCTCGTGCTC TTCCTCCACC TGGTGCTGGA TAAGCTCTTC 
CAGCTCTCTG TGCAGCCCAT GGTCATCGCT GGCCAAACAG CAAACTTCTC CCAGTTTGCC 
TTCGAATCTG TGGTGGCCAT TGCCAATAGC CTTCACAACA GCAAGGACCT GAGGAAGGAC 
CAGCACGGGA GGAACTGCCT GCTGGCCTCC TATGTGCACT ACGTGTTCCG GCTGCCCGAG 
CTGCACAGGG ATGCACCCAA GTCAGGTGGC CCCACCGCCG TAGTCCCTGA CCCCCGATAC 
CACACATATG GACGCACGTC TGCCGCTGCA GTGAGTTCAA AGCTGATGCA GGCCCGTGTG 
ATGAGCAGCA GTAACCCAGA CCTGGCTGGG ACACACTGTG CAGCAGATGA GGAGGTTAAG 
AACATCATGT CTTCCAAGAT TGCTGATCGC AACTGCAGCC GGATGTCTTA CTATTGCTCT 
GGCAACAGTG ACGTTCCAGG TTCAACTGCA GCCCCAAGGC CAGTCAGCAA AAAGCATTTC 
CATGAGGAGC TTGCCCTGCA GATGGTGGTC AGCACTGGAC TAGTGAGAGA AACCGTCTTC 
AAGTACGCCT GGTTCTTCTT TGAGCTTCTG GTGAAAAGCA TGGCACAGTA CGTCCATAAC 
CTGGATAAAC GGGACAGTTT TCGGAGGACT CGTTTTTCCG ACCGCTTCAA GGATGACATA 
ACTACCATTG TTAATGTGGT CACCTCGGAG ATAGCAGCCC TTCTAGTGAA ACCTCAGAAG 
GAAAGTGAGC AGGCCGAGAA GATCAACATC AGCCTGGCCT TCTTCCTGTA TGACCTCCTC 
TCAATCATGG ACAGAGGCTT CGTGTTCAAC CTCATCAAGC ATTACTGCAC TCAGCTATCA 
GCCAAGCTTA ACACCCTTCC AACCCTCATT TCCATGCGGC TGGAGTTCCT GAGAATCCTC 
TGCAGCCACG AGCACTACCT CAACTTGAAC CTGCTCTTCA TGAATCCTGA CACCGCACCA 
GCCTCTCCCT GTCCCTCCAT ATCCTCCCAG AACTCAAGCT CCTGCTCCAG TTTCCAGGAC 
CAAAAGATTG CCAGCATGTT CGATCTGACC CCAGAGTACC GCCAGCAGCA CTTCCTCACA 
GGGCTCCTCT TCACAGAACT GGCCGTTGCC CTGGATGCTG AAGGGGACGG AATTAGCAGA 
GTACAGAGAA AAGCCGTGAG TGCCATCCAC AGCCTTCTGA GTTCTCACGA CCTGGATCCA 
CGGTGTCTCA AACCAGAAGT GAAAGTCAAA ATCGCTGCCC TTTACCTGCC GTTGGTCGGC 
ATCATTCTGG ATGCGCTGCC ACAGCTCTAT GACTTTACAG ATGCTCGCAG TGGAAGGAGC 
CGTGCCAGTG GCTCATATGA AGAACAAGAT GTGGCCAATG GAATCAACCA GAATGTGGCC 
CTGGCCATAG CAGGGAATCA TTTTAATTTG AAGACCAGCG GAGCAATGCT GTCTTCCTTG 
CCCTATAAGC AGTACAACAT GCTGAACGCG GACACTACCC GCCACCTCAT GGTCTGCTTT 
CTGTGGATCA TGAAAAACGC GGATCAGAGC CTCATCAGGA AGTGGATCGC CGACCTGCCT 
TCCATGCAGC TCAACAGGAT TCTAGACCTG CTGTTCATCT GTGTCTCCTG CTTTGAATAC 
AAGGGAAAGC AGAGTTCTGA CAAAGTCAGT AACCAGGTCC TGCAGAAGTC AAGAGATGTC 
AAGGCCAAGT TGGAAGAAGC CCTGCTCCGT GGGGAAGGAG CCCGGGGGGA GATGATGCGA 
CGTCGGATTC CAGGCACTGA CCGGTTTCCA GGCCTAAATG AGAATCTGAG GTGGAGGAAG 
GAGCAGACAC AGTGGCGGCA AGCTAACGAG AAGCTGGACA AAACAAAGGC AGAGTTAGAT 
CAAGAAGCCT TGATCAGTGG CAACCTGGCT ACAGAGGCTA ATTTGATCAT ACTGGACATG 
CAGGAAAACA TCATCCAGGC AAGCTCCTCC CTGGACTGTA AAGACAGCCT GCTGGGAGGT 
GTCCTCCGGG TCCTGGTGAA TTCTCTGAGC TGTGATCAGA GCACCACCTA CCTGACTCAC 
TGTTTTGCAA CTCTCCGAGC CCTCATCGCC AAGTTTGGAG ACCTGCTGTT CGAGGAGGAA 
ATGGAGCAGT GTGCTGACCT GTGTCAGCGG GTGCTCCATC ACTGCAGCAG TAGCATGGAT 
GTCACCCGGA GCCAAGCCTG CGCCACCCTC TATCTACTCA TGCGGTTCAG CTTCGGAGCC 
ACCAGTAACT TTGCAAGAGT AAAGATGCAG GTAACCATGG CACTGGCATC CTTGGTAGGC 
AAAGCACCAG ACTTCAATGA GGAGCACCTG AGAAAGTCCT TAAGGACCAT TCTGGCCTAT 
TCAGAAGAGG ACACGGCCAT GCGGGCAACT CTTTTTCCCA CGCAGGTGGA GGAGCTTCTC 
TGCAATCTCA ACAGCATTTT ATACGACACA GTGAAGATGA GGGAATTCCA GGAGGACCCT 
GAGATGCTCA TGGACCTCAT GTACAGAATT GCCAAGAGCT ACCAGGCATC CCCTGACCTG 
CGGCTGACCT GGCTCCAGAA CATGGCAGAG AAACACACTA AGAGGAAGTG CTTCACAGAG 
GCCGCCATGT GCCTGGTGCA TGCAGCAGCC TTGGTGGCAG AGTACCTGAG CATGCTGGAG 
GACCACAGTT ACCTGCCTGT GGGCAGCGTC AGCTTTCAGA ATATTTCTTC CAATGTGCTT 
GAGGAATCTG CAGTCTCCGA TGACACCTTG TCACCTGATG AGGACGGTGT ATGCTCTGGT 
CGCTACTTCA CTGAGAGTGG CCTGGTGGGC CTCCTGGAGC AGGCTGCAGA GCTCTTCAGC 
ACGGGAGGCT TGTACGAGAC AGTTAATGAG GTCTACAAGC TGGTCATTCC CATCCTGGAA 
GCACATAGAG ATTTCCGGAA GCTGACCTCT ACTCACGACA AGCTGCAGAA GGCCTTTGAC 
AACATCATCA ACAAGGACCA TAAGAGGATG TTTGGAACTT ACTTCCGAGT TGGTTTCTAC 
GGATCCAAAT TTGGGGATTT GGATGAGCAG GAATTTGTGT ACAAGGAACC TGCAATTACA 
AAGCTCCCCG AGATCTCACA TAGACTAGAG GGATTTTATG GCCAGTGTTT TGGTGCCGAG 
TTTGTGGAAG TGATAAAGGA CTCTACTCCC GTGGATAAAA CCAAGTTGGA TCCTAACAAG 
GCCTACATAC AGATCACTTT TGTGGAGCCT TACTTTGATG AGTATGAGAT GAAAGACAGG 
GTGACCTACT TCGAGAAGAA TTTCAACCTT CGGAGGTTCA TGTACACCAC CCCCTTCACC 
CTGGAGGGGA GGCCCCGGGG AGAGCTGCAC GAGCAGCATC GCAGGAACAC GGTGCTCACC 
ACCATGCATG CCTTCCCCTA CATCAAGACC AGGATCCGAG TCAGCCAGAA AGAGGAGTTC 
GTCTTGACTC CGATTGAAGT TGCCATTGAA GACATGAAGA AGAAGACCTT GCAGTTAGCG 
GTGGCCACTC ACCAGGAGCC CCCTGACGCG AAGATGCTCC AGATGGTACT GCAAGGCTCT 
GTGGGAGCCA CCGTGAATCA GGGCCCACTG GAGGTGGCCC AAGTGTTCTT GGCTGAAATT 
CCGGCTGACC CAAAACTCTA CCGGCATCAC AACAAGCTGA GGTTATGCTT CAAGGAGTTC 
ATAATGCGAT GCGGTGAGGC CGTAGAGAAG AACAGGAGAC TCATTACCGC AGAGCAGCGG 
GAGTACCAGC AGGAACTCAA GAAGAACTAC AACAGGCTGA AGGACAGCCT CAGGCCCATG 
ATTGAGCGGA AAATCCCAGA GCTCTACAAG CCCATATTCA GGGTTGACAG TCAAAAGAGG 
GACTCTTTCC ACAGATCTAG TTTCAGGAAA TGTGAAACCC AGTTGTCACA GGGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032882.2
CDS	120..6419
Translation	MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSLSTSGFPSLQLPQLYEPVEPVDFEGLVMTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDERLENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERESTDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAGTHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	NM_001037793.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037793.2

ORF Insert Sequence:

ATGGCCACGC TACCGAGCGC CGAGCGCCGC GCCTTCGCGC TCAAGATCAA CAGGTATTCA 
TCATCAGAAA TAAGGAAGCA GTTTACGCTC CCGCCCAACC TCGGACAGTA CCATCGGCAC 
AGCCTCAGTA CCTCTGGTTT CCCTTCTCTT CAGCTACCTC AGCTGTATGA GCCCGTTGAG 
CCTGTGGACT TTGAAGGACT TGTGATGACG CACTTAAACA GCTTGGATGC AGAGCTGGCC 
CAGGAGCTGG GGGACCTCAC CGATGACGAC CTGCATGTGG CCTTCACACC CAAGGAGTGT 
AGGACTTTGC AGCACTCTCT GCCAGAGGAA GGAGTTGAAC TGGATCCTCA CGTCAGGGAC 
TGTGTTCAGA CCTATATTCG AGAGTGGCTG ATTGTGAACC GGAAAAACCA AGGAAGTCCA 
GAGTTTTGCA GCTTCAGAAA GACTGGATCT CGCAGAGATT TTCACAAGAC GCTTCAGAAA 
CAGACGTTTG AGTCAGAAAC CTTGGAGTGC AGTGAACCGG CCACTCAGAC GGGACCCCGC 
CATCGCTTCA ACGTGCTGTG TGATGTGTCT GGGAAGGGCC CCATCACTTC TTGTGACTTC 
GACCTCCGCA GCCTGCAGCC TGACGAGCGG CTGGAAAACC TGCTTCAGCT GAGTGCTGAG 
GACTTTGAGA AGGAGAAAGA GGAGGCCCGA AAGACCAATC GGACGGCTGA GCTCTTCGCC 
CTCTATCCAT CCGTAGATGA GGAGGATGCT GTGGAAATCC GTCCAGTACC AGAATGTCCT 
AAGGAACATC TGGGCAACAG AATATTGGTG AAGGTGCTGA CCCTGAAGTT TGAGATTGAA 
ATTGAACCTC TGTTTGCCAG TATTGCCCTC TATGATGTTA AAGAAAGGAA AAAGATCTCA 
GAAAACTTCC ACTGTGACCT GAACTCCGAC CAGTTCAAAG GGTTTCTGCG AGCTCACACA 
CCCTCGGTTG ACCCGTCGAG TCAGGCAAGG TCTGCAGTGT TCTCGGTCAC CTACCCATCT 
TCAGACATCT ACCTGGTTGT CAAGATTGAA AAGGTCCTTC AGCAAGGAGA GATCGCAGAC 
TGTGCAGAAC CCTACATGAT CATCAAAGAA AGCGATGGTG GAAAGAGTAA AGAAAAGGTT 
GAAAAACTAA AACTCCAAGC TGAATCCTTC TGCCAACGTT TGGGGAAATA CCGGATGCCC 
TTCGCCTGGG CCCCCATCAG CTTAGCAAGC TTCTTCAACG TCTCCACCCT TGAAAGGGAG 
AGCACAGACT TGGAGCCTGG GGCTGGGAGA AACTCTGTGG GGGAGAGGAG GAGTTTGTCC 
CAGTCCAGGA GGCCTTCTGA GAGAACCCTC TCCTTTGAGG AAAATGGAGT TGGATCCAAC 
TTCAAAACCA CCACCTTGGC CACTAACATC TTCTTCAAGC AGGAGGGAGA TCGCCTTAGT 
GATGAAGACT TGTTCAAGTT TTTAGCTGAC TACAAGAGAT CTTCGTCCCT ACAGCGAAGA 
GTGAAATCCA TCCCAGGCTC ACTTAGGCTG GAGATAGCCC CAGCTCCTGA CGTGATGAAC 
TGCTGCCTGA CACCCGAGAT GCTGCCGGTC AAACCCTTCC CTGAAAATCG GACGCGTCCC 
CACAAGGAGA TTTTGGAATT TCCAATCCGG GAAGTGTATG TTCCCCACAC CGTGTACAGA 
AACCTTCTGT ACGTATACCC ACAGAGACTG AACTTTGCTA ACAAGCTAGC ATCCGCCCGG 
AACATTACAA TAAAGATTCA GTTTATGTGT GGAGAAGACC CCAGCAATGC TATGCCTGTC 
ATCTTTGGGA AGTCCAGTGG GCCTGAATTT CTGCAGGAAG TGTACACAGC TATTACATAC 
CATAATAAGT CTCCCGACTT TTATGAAGAA GTAAAGATTA AGCTTCCGGC CAAGCTCACA 
GTGAATCACC ACCTCCTCTT CACCTTCTAC CACATCAGCT GTCAGCAGAA GCAAGGCGCC 
TCTGGAGAAA GCCTTCTGGG ATACTCGTGG CTGCCGATTC TCCTGAACGA ACGTCTTCAA 
ACCGGATCCT ACTGTCTACC AGTTGCCTTG GAAAAACTAC CACCTAACTA CTCCATACAT 
TCTGCTGAGA AAGTCCCTTT ACAGAATCCT CCCATTAAGT GGGCCGAGGG CCATAAGGGG 
GTATTTAATA TTGAAGTGCA AGCTGTTTCC TCCGTTCACA CCCAGGATAA CCACCTGGAG 
AAGTTCTTCA CCCTTTGCCA CTCCCTGGAG AGCCAGGTGA CCTTCCCTAT CCGCGTGCTG 
GACCAGAAGA TCACGGAGAA CACGCTGGAG CATGAGCTTA AGCTCAGCAT CATCTGCCTC 
AACTCCTCCC GCCTGGAGCC CCTCGTGCTC TTCCTCCACC TGGTGCTGGA TAAGCTCTTC 
CAGCTCTCTG TGCAGCCCAT GGTCATCGCT GGCCAAACAG CAAACTTCTC CCAGTTTGCC 
TTCGAATCTG TGGTGGCCAT TGCCAATAGC CTTCACAACA GCAAGGACCT GAGGAAGGAC 
CAGCACGGGA GGAACTGCCT GCTGGCCTCC TATGTGCACT ACGTGTTCCG GCTGCCCGAG 
CTGCACAGGG ATGCACCCAA GTCAGGTGGC CCCACCGCCG TAGTCCCTGA CCCCCGATAC 
CACACATATG GACGCACGTC TGCCGCTGCA GTGAGTTCAA AGCTGATGCA GGCCCGTGTG 
ATGAGCAGCA GTAACCCAGA CCTGGCTGGG ACACACTGTG CAGCAGATGA GGAGGTTAAG 
AACATCATGT CTTCCAAGAT TGCTGATCGC AACTGCAGCC GGATGTCTTA CTATTGCTCT 
GGCAACAGTG ACGTTCCAGG TTCAACTGCA GCCCCAAGGC CAGTCAGCAA AAAGCATTTC 
CATGAGGAGC TTGCCCTGCA GATGGTGGTC AGCACTGGAC TAGTGAGAGA AACCGTCTTC 
AAGTACGCCT GGTTCTTCTT TGAGCTTCTG GTGAAAAGCA TGGCACAGTA CGTCCATAAC 
CTGGATAAAC GGGACAGTTT TCGGAGGACT CGTTTTTCCG ACCGCTTCAA GGATGACATA 
ACTACCATTG TTAATGTGGT CACCTCGGAG ATAGCAGCCC TTCTAGTGAA ACCTCAGAAG 
GAAAGTGAGC AGGCCGAGAA GATCAACATC AGCCTGGCCT TCTTCCTGTA TGACCTCCTC 
TCAATCATGG ACAGAGGCTT CGTGTTCAAC CTCATCAAGC ATTACTGCAC TCAGCTATCA 
GCCAAGCTTA ACACCCTTCC AACCCTCATT TCCATGCGGC TGGAGTTCCT GAGAATCCTC 
TGCAGCCACG AGCACTACCT CAACTTGAAC CTGCTCTTCA TGAATCCTGA CACCGCACCA 
GCCTCTCCCT GTCCCTCCAT ATCCTCCCAG AACTCAAGCT CCTGCTCCAG TTTCCAGGAC 
CAAAAGATTG CCAGCATGTT CGATCTGACC CCAGAGTACC GCCAGCAGCA CTTCCTCACA 
GGGCTCCTCT TCACAGAACT GGCCGTTGCC CTGGATGCTG AAGGGGACGG AATTAGCAGA 
GTACAGAGAA AAGCCGTGAG TGCCATCCAC AGCCTTCTGA GTTCTCACGA CCTGGATCCA 
CGGTGTCTCA AACCAGAAGT GAAAGTCAAA ATCGCTGCCC TTTACCTGCC GTTGGTCGGC 
ATCATTCTGG ATGCGCTGCC ACAGCTCTAT GACTTTACAG ATGCTCGCAG TGGAAGGAGC 
CGTGCCAGTG GCTCATATGA AGAACAAGAT GTGGCCAATG GAATCAACCA GAATGTGGCC 
CTGGCCATAG CAGGGAATCA TTTTAATTTG AAGACCAGCG GAGCAATGCT GTCTTCCTTG 
CCCTATAAGC AGTACAACAT GCTGAACGCG GACACTACCC GCCACCTCAT GGTCTGCTTT 
CTGTGGATCA TGAAAAACGC GGATCAGAGC CTCATCAGGA AGTGGATCGC CGACCTGCCT 
TCCATGCAGC TCAACAGGAT TCTAGACCTG CTGTTCATCT GTGTCTCCTG CTTTGAATAC 
AAGGGAAAGC AGAGTTCTGA CAAAGTCAGT AACCAGGTCC TGCAGAAGTC AAGAGATGTC 
AAGGCCAAGT TGGAAGAAGC CCTGCTCCGT GGGGAAGGAG CCCGGGGGGA GATGATGCGA 
CGTCGGATTC CAGGCACTGA CCGGTTTCCA GGCCTAAATG AGAATCTGAG GTGGAGGAAG 
GAGCAGACAC AGTGGCGGCA AGCTAACGAG AAGCTGGACA AAACAAAGGC AGAGTTAGAT 
CAAGAAGCCT TGATCAGTGG CAACCTGGCT ACAGAGGCTA ATTTGATCAT ACTGGACATG 
CAGGAAAACA TCATCCAGGC AAGCTCCTCC CTGGACTGTA AAGACAGCCT GCTGGGAGGT 
GTCCTCCGGG TCCTGGTGAA TTCTCTGAGC TGTGATCAGA GCACCACCTA CCTGACTCAC 
TGTTTTGCAA CTCTCCGAGC CCTCATCGCC AAGTTTGGAG ACCTGCTGTT CGAGGAGGAA 
ATGGAGCAGT GTGCTGACCT GTGTCAGCGG GTGCTCCATC ACTGCAGCAG TAGCATGGAT 
GTCACCCGGA GCCAAGCCTG CGCCACCCTC TATCTACTCA TGCGGTTCAG CTTCGGAGCC 
ACCAGTAACT TTGCAAGAGT AAAGATGCAG GTAACCATGG CACTGGCATC CTTGGTAGGC 
AAAGCACCAG ACTTCAATGA GGAGCACCTG AGAAAGTCCT TAAGGACCAT TCTGGCCTAT 
TCAGAAGAGG ACACGGCCAT GCGGGCAACT CTTTTTCCCA CGCAGGTGGA GGAGCTTCTC 
TGCAATCTCA ACAGCATTTT ATACGACACA GTGAAGATGA GGGAATTCCA GGAGGACCCT 
GAGATGCTCA TGGACCTCAT GTACAGAATT GCCAAGAGCT ACCAGGCATC CCCTGACCTG 
CGGCTGACCT GGCTCCAGAA CATGGCAGAG AAACACACTA AGAGGAAGTG CTTCACAGAG 
GCCGCCATGT GCCTGGTGCA TGCAGCAGCC TTGGTGGCAG AGTACCTGAG CATGCTGGAG 
GACCACAGTT ACCTGCCTGT GGGCAGCGTC AGCTTTCAGA ATATTTCTTC CAATGTGCTT 
GAGGAATCTG CAGTCTCCGA TGACACCTTG TCACCTGATG AGGACGGTGT ATGCTCTGGT 
CGCTACTTCA CTGAGAGTGG CCTGGTGGGC CTCCTGGAGC AGGCTGCAGA GCTCTTCAGC 
ACGGGAGGCT TGTACGAGAC AGTTAATGAG GTCTACAAGC TGGTCATTCC CATCCTGGAA 
GCACATAGAG ATTTCCGGAA GCTGACCTCT ACTCACGACA AGCTGCAGAA GGCCTTTGAC 
AACATCATCA ACAAGGACCA TAAGAGGATG TTTGGAACTT ACTTCCGAGT TGGTTTCTAC 
GGATCCAAAT TTGGGGATTT GGATGAGCAG GAATTTGTGT ACAAGGAACC TGCAATTACA 
AAGCTCCCCG AGATCTCACA TAGACTAGAG GGATTTTATG GCCAGTGTTT TGGTGCCGAG 
TTTGTGGAAG TGATAAAGGA CTCTACTCCC GTGGATAAAA CCAAGTTGGA TCCTAACAAG 
GCCTACATAC AGATCACTTT TGTGGAGCCT TACTTTGATG AGTATGAGAT GAAAGACAGG 
GTGACCTACT TCGAGAAGAA TTTCAACCTT CGGAGGTTCA TGTACACCAC CCCCTTCACC 
CTGGAGGGGA GGCCCCGGGG AGAGCTGCAC GAGCAGCATC GCAGGAACAC GGTGCTCACC 
ACCATGCATG CCTTCCCCTA CATCAAGACC AGGATCCGAG TCAGCCAGAA AGAGGAGTTC 
GTCTTGACTC CGATTGAAGT TGCCATTGAA GACATGAAGA AGAAGACCTT GCAGTTAGCG 
GTGGCCACTC ACCAGGAGCC CCCTGACGCG AAGATGCTCC AGATGGTACT GCAAGGCTCT 
GTGGGAGCCA CCGTGAATCA GGGCCCACTG GAGGTGGCCC AAGTGTTCTT GGCTGAAATT 
CCGGCTGACC CAAAACTCTA CCGGCATCAC AACAAGCTGA GGTTATGCTT CAAGGAGTTC 
ATAATGCGAT GCGGTGAGGC CGTAGAGAAG AACAGGAGAC TCATTACCGC AGAGCAGCGG 
GAGTACCAGC AGGAACTCAA GAAGAACTAC AACAGGCTGA AGGACAGCCT CAGGCCCATG 
ATTGAGCGGA AAATCCCAGA GCTCTACAAG CCCATATTCA GGGTTGACAG TCAAAAGAGG 
GACTCTTTCC ACAGATCTAG TTTCAGGAAA TGTGAAACCC AGTTGTCACA GGGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_006231245.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231307.1 Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

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CloneID	ORa24021
Clone ID Related Accession (Same CDS sequence)	XM_006231245.2 , XM_006231244.3 , XM_008760310.2 , XM_008760311.2
Accession Version	XM_006231245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231307.1
CDS	1099..7194
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDERLENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERESTDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAGTHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_006231245.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231245.2

ORF Insert Sequence:

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_006231244.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231306.1 Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.

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CloneID	ORa24021
Clone ID Related Accession (Same CDS sequence)	XM_006231245.2 , XM_006231244.3 , XM_008760310.2 , XM_008760311.2
Accession Version	XM_006231244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231306.1
CDS	532..6627
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDERLENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERESTDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAGTHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_006231244.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231244.3

ORF Insert Sequence:

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_008760310.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758532.1 Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

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CloneID	ORa24021
Clone ID Related Accession (Same CDS sequence)	XM_006231245.2 , XM_006231244.3 , XM_008760310.2 , XM_008760311.2
Accession Version	XM_008760310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758532.1
CDS	587..6682
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDERLENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERESTDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAGTHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_008760310.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760310.2

ORF Insert Sequence:

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dock8 ( XM_008760311.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758533.1 Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X4, mRNA.

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CloneID	ORa24021
Clone ID Related Accession (Same CDS sequence)	XM_006231245.2 , XM_006231244.3 , XM_008760310.2 , XM_008760311.2
Accession Version	XM_008760311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758533.1
CDS	197..6292
Translation	MTHLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSPEFCSFRKTGSRRDFHKTLQKQTFESETLECSEPATQTGPRHRFNVLCDVSGKGPITSCDFDLRSLQPDERLENLLQLSAEDFEKEKEEARKTNRTAELFALYPSVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEIEIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSVDPSSQARSAVFSVTYPSSDIYLVVKIEKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNVSTLERESTDLEPGAGRNSVGERRSLSQSRRPSERTLSFEENGVGSNFKTTTLATNIFFKQEGDRLSDEDLFKFLADYKRSSSLQRRVKSIPGSLRLEIAPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVYRNLLYVYPQRLNFANKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSIHSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVTFPIRVLDQKITENTLEHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRKDQHGRNCLLASYVHYVFRLPELHRDAPKSGGPTAVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLAGTHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDVPGSTAAPRPVSKKHFHEELALQMVVSTGLVRETVFKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKINISLAFFLYDLLSIMDRGFVFNLIKHYCTQLSAKLNTLPTLISMRLEFLRILCSHEHYLNLNLLFMNPDTAPASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAIHSLLSSHDLDPRCLKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNVALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMVCFLWIMKNADQSLIRKWIADLPSMQLNRILDLLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGLNENLRWRKEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSSLDCKDSLLGGVLRVLVNSLSCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFGATSNFARVKMQVTMALASLVGKAPDFNEEHLRKSLRTILAYSEEDTAMRATLFPTQVEELLCNLNSILYDTVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKRKCFTEAAMCLVHAAALVAEYLSMLEDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVNEVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSKFGDLDEQEFVYKEPAITKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFNLRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQLAVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNRRLITAEQREYQQELKKNYNRLKDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS

Target ORF information:

RefSeq Version	XM_008760311.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dedicator of cytokinesis 8 (Dock8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760311.2

ORF Insert Sequence:

ATGACGCACT TAAACAGCTT GGATGCAGAG CTGGCCCAGG AGCTGGGGGA CCTCACCGAT 
GACGACCTGC ATGTGGCCTT CACACCCAAG GAGTGTAGGA CTTTGCAGCA CTCTCTGCCA 
GAGGAAGGAG TTGAACTGGA TCCTCACGTC AGGGACTGTG TTCAGACCTA TATTCGAGAG 
TGGCTGATTG TGAACCGGAA AAACCAAGGA AGTCCAGAGT TTTGCAGCTT CAGAAAGACT 
GGATCTCGCA GAGATTTTCA CAAGACGCTT CAGAAACAGA CGTTTGAGTC AGAAACCTTG 
GAGTGCAGTG AACCGGCCAC TCAGACGGGA CCCCGCCATC GCTTCAACGT GCTGTGTGAT 
GTGTCTGGGA AGGGCCCCAT CACTTCTTGT GACTTCGACC TCCGCAGCCT GCAGCCTGAC 
GAGCGGCTGG AAAACCTGCT TCAGCTGAGT GCTGAGGACT TTGAGAAGGA GAAAGAGGAG 
GCCCGAAAGA CCAATCGGAC GGCTGAGCTC TTCGCCCTCT ATCCATCCGT AGATGAGGAG 
GATGCTGTGG AAATCCGTCC AGTACCAGAA TGTCCTAAGG AACATCTGGG CAACAGAATA 
TTGGTGAAGG TGCTGACCCT GAAGTTTGAG ATTGAAATTG AACCTCTGTT TGCCAGTATT 
GCCCTCTATG ATGTTAAAGA AAGGAAAAAG ATCTCAGAAA ACTTCCACTG TGACCTGAAC 
TCCGACCAGT TCAAAGGGTT TCTGCGAGCT CACACACCCT CGGTTGACCC GTCGAGTCAG 
GCAAGGTCTG CAGTGTTCTC GGTCACCTAC CCATCTTCAG ACATCTACCT GGTTGTCAAG 
ATTGAAAAGG TCCTTCAGCA AGGAGAGATC GCAGACTGTG CAGAACCCTA CATGATCATC 
AAAGAAAGCG ATGGTGGAAA GAGTAAAGAA AAGGTTGAAA AACTAAAACT CCAAGCTGAA 
TCCTTCTGCC AACGTTTGGG GAAATACCGG ATGCCCTTCG CCTGGGCCCC CATCAGCTTA 
GCAAGCTTCT TCAACGTCTC CACCCTTGAA AGGGAGAGCA CAGACTTGGA GCCTGGGGCT 
GGGAGAAACT CTGTGGGGGA GAGGAGGAGT TTGTCCCAGT CCAGGAGGCC TTCTGAGAGA 
ACCCTCTCCT TTGAGGAAAA TGGAGTTGGA TCCAACTTCA AAACCACCAC CTTGGCCACT 
AACATCTTCT TCAAGCAGGA GGGAGATCGC CTTAGTGATG AAGACTTGTT CAAGTTTTTA 
GCTGACTACA AGAGATCTTC GTCCCTACAG CGAAGAGTGA AATCCATCCC AGGCTCACTT 
AGGCTGGAGA TAGCCCCAGC TCCTGACGTG ATGAACTGCT GCCTGACACC CGAGATGCTG 
CCGGTCAAAC CCTTCCCTGA AAATCGGACG CGTCCCCACA AGGAGATTTT GGAATTTCCA 
ATCCGGGAAG TGTATGTTCC CCACACCGTG TACAGAAACC TTCTGTACGT ATACCCACAG 
AGACTGAACT TTGCTAACAA GCTAGCATCC GCCCGGAACA TTACAATAAA GATTCAGTTT 
ATGTGTGGAG AAGACCCCAG CAATGCTATG CCTGTCATCT TTGGGAAGTC CAGTGGGCCT 
GAATTTCTGC AGGAAGTGTA CACAGCTATT ACATACCATA ATAAGTCTCC CGACTTTTAT 
GAAGAAGTAA AGATTAAGCT TCCGGCCAAG CTCACAGTGA ATCACCACCT CCTCTTCACC 
TTCTACCACA TCAGCTGTCA GCAGAAGCAA GGCGCCTCTG GAGAAAGCCT TCTGGGATAC 
TCGTGGCTGC CGATTCTCCT GAACGAACGT CTTCAAACCG GATCCTACTG TCTACCAGTT 
GCCTTGGAAA AACTACCACC TAACTACTCC ATACATTCTG CTGAGAAAGT CCCTTTACAG 
AATCCTCCCA TTAAGTGGGC CGAGGGCCAT AAGGGGGTAT TTAATATTGA AGTGCAAGCT 
GTTTCCTCCG TTCACACCCA GGATAACCAC CTGGAGAAGT TCTTCACCCT TTGCCACTCC 
CTGGAGAGCC AGGTGACCTT CCCTATCCGC GTGCTGGACC AGAAGATCAC GGAGAACACG 
CTGGAGCATG AGCTTAAGCT CAGCATCATC TGCCTCAACT CCTCCCGCCT GGAGCCCCTC 
GTGCTCTTCC TCCACCTGGT GCTGGATAAG CTCTTCCAGC TCTCTGTGCA GCCCATGGTC 
ATCGCTGGCC AAACAGCAAA CTTCTCCCAG TTTGCCTTCG AATCTGTGGT GGCCATTGCC 
AATAGCCTTC ACAACAGCAA GGACCTGAGG AAGGACCAGC ACGGGAGGAA CTGCCTGCTG 
GCCTCCTATG TGCACTACGT GTTCCGGCTG CCCGAGCTGC ACAGGGATGC ACCCAAGTCA 
GGTGGCCCCA CCGCCGTAGT CCCTGACCCC CGATACCACA CATATGGACG CACGTCTGCC 
GCTGCAGTGA GTTCAAAGCT GATGCAGGCC CGTGTGATGA GCAGCAGTAA CCCAGACCTG 
GCTGGGACAC ACTGTGCAGC AGATGAGGAG GTTAAGAACA TCATGTCTTC CAAGATTGCT 
GATCGCAACT GCAGCCGGAT GTCTTACTAT TGCTCTGGCA ACAGTGACGT TCCAGGTTCA 
ACTGCAGCCC CAAGGCCAGT CAGCAAAAAG CATTTCCATG AGGAGCTTGC CCTGCAGATG 
GTGGTCAGCA CTGGACTAGT GAGAGAAACC GTCTTCAAGT ACGCCTGGTT CTTCTTTGAG 
CTTCTGGTGA AAAGCATGGC ACAGTACGTC CATAACCTGG ATAAACGGGA CAGTTTTCGG 
AGGACTCGTT TTTCCGACCG CTTCAAGGAT GACATAACTA CCATTGTTAA TGTGGTCACC 
TCGGAGATAG CAGCCCTTCT AGTGAAACCT CAGAAGGAAA GTGAGCAGGC CGAGAAGATC 
AACATCAGCC TGGCCTTCTT CCTGTATGAC CTCCTCTCAA TCATGGACAG AGGCTTCGTG 
TTCAACCTCA TCAAGCATTA CTGCACTCAG CTATCAGCCA AGCTTAACAC CCTTCCAACC 
CTCATTTCCA TGCGGCTGGA GTTCCTGAGA ATCCTCTGCA GCCACGAGCA CTACCTCAAC 
TTGAACCTGC TCTTCATGAA TCCTGACACC GCACCAGCCT CTCCCTGTCC CTCCATATCC 
TCCCAGAACT CAAGCTCCTG CTCCAGTTTC CAGGACCAAA AGATTGCCAG CATGTTCGAT 
CTGACCCCAG AGTACCGCCA GCAGCACTTC CTCACAGGGC TCCTCTTCAC AGAACTGGCC 
GTTGCCCTGG ATGCTGAAGG GGACGGAATT AGCAGAGTAC AGAGAAAAGC CGTGAGTGCC 
ATCCACAGCC TTCTGAGTTC TCACGACCTG GATCCACGGT GTCTCAAACC AGAAGTGAAA 
GTCAAAATCG CTGCCCTTTA CCTGCCGTTG GTCGGCATCA TTCTGGATGC GCTGCCACAG 
CTCTATGACT TTACAGATGC TCGCAGTGGA AGGAGCCGTG CCAGTGGCTC ATATGAAGAA 
CAAGATGTGG CCAATGGAAT CAACCAGAAT GTGGCCCTGG CCATAGCAGG GAATCATTTT 
AATTTGAAGA CCAGCGGAGC AATGCTGTCT TCCTTGCCCT ATAAGCAGTA CAACATGCTG 
AACGCGGACA CTACCCGCCA CCTCATGGTC TGCTTTCTGT GGATCATGAA AAACGCGGAT 
CAGAGCCTCA TCAGGAAGTG GATCGCCGAC CTGCCTTCCA TGCAGCTCAA CAGGATTCTA 
GACCTGCTGT TCATCTGTGT CTCCTGCTTT GAATACAAGG GAAAGCAGAG TTCTGACAAA 
GTCAGTAACC AGGTCCTGCA GAAGTCAAGA GATGTCAAGG CCAAGTTGGA AGAAGCCCTG 
CTCCGTGGGG AAGGAGCCCG GGGGGAGATG ATGCGACGTC GGATTCCAGG CACTGACCGG 
TTTCCAGGCC TAAATGAGAA TCTGAGGTGG AGGAAGGAGC AGACACAGTG GCGGCAAGCT 
AACGAGAAGC TGGACAAAAC AAAGGCAGAG TTAGATCAAG AAGCCTTGAT CAGTGGCAAC 
CTGGCTACAG AGGCTAATTT GATCATACTG GACATGCAGG AAAACATCAT CCAGGCAAGC 
TCCTCCCTGG ACTGTAAAGA CAGCCTGCTG GGAGGTGTCC TCCGGGTCCT GGTGAATTCT 
CTGAGCTGTG ATCAGAGCAC CACCTACCTG ACTCACTGTT TTGCAACTCT CCGAGCCCTC 
ATCGCCAAGT TTGGAGACCT GCTGTTCGAG GAGGAAATGG AGCAGTGTGC TGACCTGTGT 
CAGCGGGTGC TCCATCACTG CAGCAGTAGC ATGGATGTCA CCCGGAGCCA AGCCTGCGCC 
ACCCTCTATC TACTCATGCG GTTCAGCTTC GGAGCCACCA GTAACTTTGC AAGAGTAAAG 
ATGCAGGTAA CCATGGCACT GGCATCCTTG GTAGGCAAAG CACCAGACTT CAATGAGGAG 
CACCTGAGAA AGTCCTTAAG GACCATTCTG GCCTATTCAG AAGAGGACAC GGCCATGCGG 
GCAACTCTTT TTCCCACGCA GGTGGAGGAG CTTCTCTGCA ATCTCAACAG CATTTTATAC 
GACACAGTGA AGATGAGGGA ATTCCAGGAG GACCCTGAGA TGCTCATGGA CCTCATGTAC 
AGAATTGCCA AGAGCTACCA GGCATCCCCT GACCTGCGGC TGACCTGGCT CCAGAACATG 
GCAGAGAAAC ACACTAAGAG GAAGTGCTTC ACAGAGGCCG CCATGTGCCT GGTGCATGCA 
GCAGCCTTGG TGGCAGAGTA CCTGAGCATG CTGGAGGACC ACAGTTACCT GCCTGTGGGC 
AGCGTCAGCT TTCAGAATAT TTCTTCCAAT GTGCTTGAGG AATCTGCAGT CTCCGATGAC 
ACCTTGTCAC CTGATGAGGA CGGTGTATGC TCTGGTCGCT ACTTCACTGA GAGTGGCCTG 
GTGGGCCTCC TGGAGCAGGC TGCAGAGCTC TTCAGCACGG GAGGCTTGTA CGAGACAGTT 
AATGAGGTCT ACAAGCTGGT CATTCCCATC CTGGAAGCAC ATAGAGATTT CCGGAAGCTG 
ACCTCTACTC ACGACAAGCT GCAGAAGGCC TTTGACAACA TCATCAACAA GGACCATAAG 
AGGATGTTTG GAACTTACTT CCGAGTTGGT TTCTACGGAT CCAAATTTGG GGATTTGGAT 
GAGCAGGAAT TTGTGTACAA GGAACCTGCA ATTACAAAGC TCCCCGAGAT CTCACATAGA 
CTAGAGGGAT TTTATGGCCA GTGTTTTGGT GCCGAGTTTG TGGAAGTGAT AAAGGACTCT 
ACTCCCGTGG ATAAAACCAA GTTGGATCCT AACAAGGCCT ACATACAGAT CACTTTTGTG 
GAGCCTTACT TTGATGAGTA TGAGATGAAA GACAGGGTGA CCTACTTCGA GAAGAATTTC 
AACCTTCGGA GGTTCATGTA CACCACCCCC TTCACCCTGG AGGGGAGGCC CCGGGGAGAG 
CTGCACGAGC AGCATCGCAG GAACACGGTG CTCACCACCA TGCATGCCTT CCCCTACATC 
AAGACCAGGA TCCGAGTCAG CCAGAAAGAG GAGTTCGTCT TGACTCCGAT TGAAGTTGCC 
ATTGAAGACA TGAAGAAGAA GACCTTGCAG TTAGCGGTGG CCACTCACCA GGAGCCCCCT 
GACGCGAAGA TGCTCCAGAT GGTACTGCAA GGCTCTGTGG GAGCCACCGT GAATCAGGGC 
CCACTGGAGG TGGCCCAAGT GTTCTTGGCT GAAATTCCGG CTGACCCAAA ACTCTACCGG 
CATCACAACA AGCTGAGGTT ATGCTTCAAG GAGTTCATAA TGCGATGCGG TGAGGCCGTA 
GAGAAGAACA GGAGACTCAT TACCGCAGAG CAGCGGGAGT ACCAGCAGGA ACTCAAGAAG 
AACTACAACA GGCTGAAGGA CAGCCTCAGG CCCATGATTG AGCGGAAAAT CCCAGAGCTC 
TACAAGCCCA TATTCAGGGT TGACAGTCAA AAGAGGGACT CTTTCCACAG ATCTAGTTTC 
AGGAAATGTG AAACCCAGTT GTCACAGGGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis.
The Journal of experimental medicine214(1)209-226(2017 Jan)
Xu X,Han L,Zhao G,Xue S,Gao Y,Xiao J,Zhang S,Chen P,Wu ZY,Ding J,Hu R,Wei B,Wang H

A novel Dock8 gene mutation confers diabetogenic susceptibility in the LEW.1AR1/Ztm-iddm rat, an animal model of human type 1 diabetes.
Diabetologia58(12)2800-9(2015 Dec)
Arndt T,Wedekind D,J?rns A,Tsiavaliaris G,Cuppen E,Hedrich HJ,Lenzen S

Coincidental loss of DOCK8 function in NLRP10-deficient and C3H/HeJ mice results in defective dendritic cell migration.
Proceedings of the National Academy of Sciences of the United States of America112(10)3056-61(2015 Mar)
Krishnaswamy JK,Singh A,Gowthaman U,Wu R,Gorrepati P,Sales Nascimento M,Gallman A,Liu D,Rhebergen AM,Calabro S,Xu L,Ranney P,Srivastava A,Ranson M,Gorham JD,McCaw Z,Kleeberger SR,Heinz LX,M?ller AC,Bennett KL,Superti-Furga G,Henao-Mejia J,Sutterwala FS,Williams A,Flavell RA,Eisenbarth SC

DOCK8 is a Cdc42 activator critical for interstitial dendritic cell migration during immune responses.
Blood119(19)4451-61(2012 May)
Harada Y,Tanaka Y,Terasawa M,Pieczyk M,Habiro K,Katakai T,Hanawa-Suetsugu K,Kukimoto-Niino M,Nishizaki T,Shirouzu M,Duan X,Uruno T,Nishikimi A,Sanematsu F,Yokoyama S,Stein JV,Kinashi T,Fukui Y

DOCK8 deficiency impairs CD8 T cell survival and function in humans and mice.
The Journal of experimental medicine208(11)2305-20(2011 Oct)
Randall KL,Chan SS,Ma CS,Fung I,Mei Y,Yabas M,Tan A,Arkwright PD,Al Suwairi W,Lugo Reyes SO,Yamazaki-Nakashimada MA,Garcia-Cruz Mde L,Smart JM,Picard C,Okada S,Jouanguy E,Casanova JL,Lambe T,Cornall RJ,Russell S,Oliaro J,Tangye SG,Bertram EM,Goodnow CC

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Dock8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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