Mapkapk5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mapkapk5
Entrez Gene ID	498183
Full Name	mitogen-activated protein kinase-activated protein kinase 5
General protein information	Preferred Names mitogen-activated protein kinase-activated protein kinase 5 Names MAP kinase-activated protein kinase 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164043.1	NP_001157515.1	MAP kinase-activated protein kinase 5 isoform 1
NM_001025761.2	NP_001020932.2	MAP kinase-activated protein kinase 5 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAPKAPK5	NP_620777.1
Pan troglodytes (chimpanzee)	MAPKAPK5	XP_003313994.2
Mus musculus (house mouse)	Mapkapk5	NP_034895.1
Rattus norvegicus (Norway rat)	Mapkapk5	NP_001157515.1
Gallus gallus (chicken)	MAPKAPK5	NP_001186736.1
	MAPKAPK5	XP_001102910.2
Canis lupus familiaris (dog)	MAPKAPK5	XP_005636302.1
Bos taurus (cattle)	MAPKAPK5	NP_001193787.1
Xenopus tropicalis (tropical clawed frog)	mapkapk5	NP_001006873.1
Danio rerio (zebrafish)	mapkapk5	NP_001002336.1

Gene Ontology
Bibliography

Related articles in PubMed

The extracellular signal-regulated kinase 3 (mitogen-activated protein kinase 6 [MAPK6])-MAPK-activated protein kinase 5 signaling complex regulates septin function and dendrite morphology.
Brand F, Schumacher S, Kant S, Menon MB, Simon R, Turgeon B, Britsch S, Meloche S, Gaestel M, Kotlyarov A
Molecular and cellular biology32(13)2467-78(2012 Jul)

High-throughput RNAi screening reveals novel regulators of telomerase.
Cerone MA, Burgess DJ, Naceur-Lombardelli C, Lord CJ, Ashworth A
Cancer research71(9)3328-40(2011 May)

The MK5/PRAK kinase and Myc form a negative feedback loop that is disrupted during colorectal tumorigenesis.
Kress TR, Cannell IG, Brenkman AB, Samans B, Gaestel M, Roepman P, Burgering BM, Bushell M, Rosenwald A, Eilers M
Molecular cell41(4)445-57(2011 Feb)

PRAK is essential for ras-induced senescence and tumor suppression.
Sun P, Yoshizuka N, New L, Moser BA, Li Y, Liao R, Xie C, Chen J, Deng Q, Yamout M, Dong MQ, Frangou CG, Yates JR 3rd, Wright PE, Han J
Cell128(2)295-308(2007 Jan)

MAPKAP kinases - MKs - two's company, three's a crowd.
Gaestel M
Nature reviews. Molecular cell biology7(2)120-30(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mapkapk5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mapkapk5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04773	NM_001164043.1 Latest version!	Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa07747	NM_001025761.2 Latest version!	Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mapkapk5 ( NM_001164043.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001157515.1 Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa04773
Clone ID Related Accession (Same CDS sequence)	NM_001164043.1
Accession Version	NM_001164043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	MAP kinase-activated protein kinase 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM032487.1, BC099161.1 and AI715466.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGG ATAGCAACAT GGAGAAAGCC ATCAAGGAGA CCTCCATTTT AGAAGAATAT 
AGTATCAATT GGACTCAGAA ACTGGGAGCT GGAATTAGTG GTCCGGTTAG AGTCTGTGTA 
AAGAAATCTA CTCAAGAACG GTTTGCACTG AAAATTCTTC TCGATCGTCC AAAAGCTAGA 
AATGAGGTGC GCCTGCACAT GATGTGTGCC ACACACTCCA ACATAGTTGA GATTATTGAA 
GTGTTTGCTA ACAGTGTACA GTTCCCTCAT GAGTCCAGCC CCAGGGCTCG ACTCTTAATT 
GTAATGGAGA TGATGGAAGG GGGCGAGCTG TTTCACAGAA TCAGCCAGCA CCGGCACTTT 
ACAGAGAAGC AAGCCAGCCA AGTAACAAAG CAGATAGCCC TGGCTCTACA GCACTGTCAC 
TTGCTAAACA TTGCGCACAG AGACCTCAAG CCTGAAAATC TGCTTTTCAA GGATAACTCT 
TTGGACGCCC CTGTGAAGTT ATGTGACTTT GGGTTTGCTA AAGTTGACCA AGGTGATTTG 
ATGACGCCCC AGTTTACCCC TTACTATGTA GCACCTCAGG TACTGGAAGC ACAGAGAAGG 
CACCAGAAGG AGAAGTCTGG CCTCATCCCT ACCTCGCCAA CGCCCTACAC TTACAACAAG 
AGCTGTGACT TGTGGTCCCT CGGGGTGATA ATTTATGTGA TGCTATGCGG ATACCCTCCT 
TTTTACTCCA AACACCACAG TCGGACCATC CCAAAGGACA TGCGGAAAAA GATCATGACA 
GGGAGTTTTG AGTTCCCGGA GGAAGAGTGG AGCCAGATCT CAGAGATGGC CAAAGACGTG 
GTGAGGAAGC TTCTAAAGGT CAAACCAGAG GAAAGACTCA CAATCGAGGG GGTGTTGGAC 
CATCCCTGGC TCAACTCGAC AGAGGCCCTG GATAATGTGC TACCCTCTGC TCAGCTGATG 
ATGGATAAGG CAGTGGTTGC AGGGATCCAG CAGGCTCATG CCGAGCAGCT GGCAAACATG 
AGGATCCAGG ACCTCAAAGT CAGCCTCAAA CCCCTGCACT CTGTGAACAA CCCCATTCTG 
AGGAAGAGGA AGCTACTGGG CTCCAAGCCA AAGGATGGTG TTTATATCCA CGACCATGAG 
AATGGAGTCG AGGATTCAAA TGTTGCCTTG GAAAAGCTTC GCGATGTCAT TGCCCAGTGC 
ATCCTTCCCC AGGCTGGTAA AGGAGAGAAT GAAGATGAGA AGCTCAATGA GGTAATGCAG 
GAGGCCTGGA AGTACAACCG GGAGTGCAAA CTCCTGCGGG ACGCGCTGCA GAGTTTTAGC 
TGGAATGGTC GTGGATTCAC AGATAAAGTG GACCGATTAA AGCTGGCAGA GGTTGTGAAG 
CAGGTGATCG AAGAGCAGAC CCTTCCCCAC GAGCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157515.1
CDS	168..1589
Translation	MSEDSNMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCATHSNIVEIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGLIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGSKPKDGVYIHDHENGVEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ

Target ORF information:

RefSeq Version	NM_001164043.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164043.1

ORF Insert Sequence:

ATGTCGGAGG ATAGCAACAT GGAGAAAGCC ATCAAGGAGA CCTCCATTTT AGAAGAATAT 
AGTATCAATT GGACTCAGAA ACTGGGAGCT GGAATTAGTG GTCCGGTTAG AGTCTGTGTA 
AAGAAATCTA CTCAAGAACG GTTTGCACTG AAAATTCTTC TCGATCGTCC AAAAGCTAGA 
AATGAGGTGC GCCTGCACAT GATGTGTGCC ACACACTCCA ACATAGTTGA GATTATTGAA 
GTGTTTGCTA ACAGTGTACA GTTCCCTCAT GAGTCCAGCC CCAGGGCTCG ACTCTTAATT 
GTAATGGAGA TGATGGAAGG GGGCGAGCTG TTTCACAGAA TCAGCCAGCA CCGGCACTTT 
ACAGAGAAGC AAGCCAGCCA AGTAACAAAG CAGATAGCCC TGGCTCTACA GCACTGTCAC 
TTGCTAAACA TTGCGCACAG AGACCTCAAG CCTGAAAATC TGCTTTTCAA GGATAACTCT 
TTGGACGCCC CTGTGAAGTT ATGTGACTTT GGGTTTGCTA AAGTTGACCA AGGTGATTTG 
ATGACGCCCC AGTTTACCCC TTACTATGTA GCACCTCAGG TACTGGAAGC ACAGAGAAGG 
CACCAGAAGG AGAAGTCTGG CCTCATCCCT ACCTCGCCAA CGCCCTACAC TTACAACAAG 
AGCTGTGACT TGTGGTCCCT CGGGGTGATA ATTTATGTGA TGCTATGCGG ATACCCTCCT 
TTTTACTCCA AACACCACAG TCGGACCATC CCAAAGGACA TGCGGAAAAA GATCATGACA 
GGGAGTTTTG AGTTCCCGGA GGAAGAGTGG AGCCAGATCT CAGAGATGGC CAAAGACGTG 
GTGAGGAAGC TTCTAAAGGT CAAACCAGAG GAAAGACTCA CAATCGAGGG GGTGTTGGAC 
CATCCCTGGC TCAACTCGAC AGAGGCCCTG GATAATGTGC TACCCTCTGC TCAGCTGATG 
ATGGATAAGG CAGTGGTTGC AGGGATCCAG CAGGCTCATG CCGAGCAGCT GGCAAACATG 
AGGATCCAGG ACCTCAAAGT CAGCCTCAAA CCCCTGCACT CTGTGAACAA CCCCATTCTG 
AGGAAGAGGA AGCTACTGGG CTCCAAGCCA AAGGATGGTG TTTATATCCA CGACCATGAG 
AATGGAGTCG AGGATTCAAA TGTTGCCTTG GAAAAGCTTC GCGATGTCAT TGCCCAGTGC 
ATCCTTCCCC AGGCTGGTAA AGGAGAGAAT GAAGATGAGA AGCTCAATGA GGTAATGCAG 
GAGGCCTGGA AGTACAACCG GGAGTGCAAA CTCCTGCGGG ACGCGCTGCA GAGTTTTAGC 
TGGAATGGTC GTGGATTCAC AGATAAAGTG GACCGATTAA AGCTGGCAGA GGTTGTGAAG 
CAGGTGATCG AAGAGCAGAC CCTTCCCCAC GAGCCCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mapkapk5 ( NM_001025761.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020932.2 Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa07747
Clone ID Related Accession (Same CDS sequence)	NM_001025761.2
Accession Version	NM_001025761.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	MAP kinase-activated protein kinase 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC099161.1 and AI715466.1. On Aug 11, 2009 this sequence version replaced NM_001025761.1. ##Evidence-Data-START## Transcript exon combination :: BC099161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632253, SAMN04563758 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATGA TGGAAGGGGG CGAGCTGTTT CACAGAATCA GCCAGCACCG GCACTTTACA 
GAGAAGCAAG CCAGCCAAGT AACAAAGCAG ATAGCCCTGG CTCTACAGCA CTGTCACTTG 
CTAAACATTG CGCACAGAGA CCTCAAGCCT GAAAATCTGC TTTTCAAGGA TAACTCTTTG 
GACGCCCCTG TGAAGTTATG TGACTTTGGG TTTGCTAAAG TTGACCAAGG TGATTTGATG 
ACGCCCCAGT TTACCCCTTA CTATGTAGCA CCTCAGGTAC TGGAAGCACA GAGAAGGCAC 
CAGAAGGAGA AGTCTGGCCT CATCCCTACC TCGCCAACGC CCTACACTTA CAACAAGAGC 
TGTGACTTGT GGTCCCTCGG GGTGATAATT TATGTGATGC TATGCGGATA CCCTCCTTTT 
TACTCCAAAC ACCACAGTCG GACCATCCCA AAGGACATGC GGAAAAAGAT CATGACAGGG 
AGTTTTGAGT TCCCGGAGGA AGAGTGGAGC CAGATCTCAG AGATGGCCAA AGACGTGGTG 
AGGAAGCTTC TAAAGGTCAA ACCAGAGGAA AGACTCACAA TCGAGGGGGT GTTGGACCAT 
CCCTGGCTCA ACTCGACAGA GGCCCTGGAT AATGTGCTAC CCTCTGCTCA GCTGATGATG 
GATAAGGCAG TGGTTGCAGG GATCCAGCAG GCTCATGCCG AGCAGCTGGC AAACATGAGG 
ATCCAGGACC TCAAAGTCAG CCTCAAACCC CTGCACTCTG TGAACAACCC CATTCTGAGG 
AAGAGGAAGC TACTGGGCTC CAAGCCAAAG GATGGTGTTT ATATCCACGA CCATGAGAAT 
GGAGTCGAGG ATTCAAATGT TGCCTTGGAA AAGCTTCGCG ATGTCATTGC CCAGTGCATC 
CTTCCCCAGG CTGGTAAAGG AGAGAATGAA GATGAGAAGC TCAATGAGGT AATGCAGGAG 
GCCTGGAAGT ACAACCGGGA GTGCAAACTC CTGCGGGACG CGCTGCAGAG TTTTAGCTGG 
AATGGTCGTG GATTCACAGA TAAAGTGGAC CGATTAAAGC TGGCAGAGGT TGTGAAGCAG 
GTGATCGAAG AGCAGACCCT TCCCCACGAG CCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020932.2
CDS	102..1220
Translation	MEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCHLLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGLIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLKPLHSVNNPILRKRKLLGSKPKDGVYIHDHENGVEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ

Target ORF information:

RefSeq Version	NM_001025761.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MAPK activated protein kinase 5 (Mapkapk5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025761.2

ORF Insert Sequence:

ATGGAGATGA TGGAAGGGGG CGAGCTGTTT CACAGAATCA GCCAGCACCG GCACTTTACA 
GAGAAGCAAG CCAGCCAAGT AACAAAGCAG ATAGCCCTGG CTCTACAGCA CTGTCACTTG 
CTAAACATTG CGCACAGAGA CCTCAAGCCT GAAAATCTGC TTTTCAAGGA TAACTCTTTG 
GACGCCCCTG TGAAGTTATG TGACTTTGGG TTTGCTAAAG TTGACCAAGG TGATTTGATG 
ACGCCCCAGT TTACCCCTTA CTATGTAGCA CCTCAGGTAC TGGAAGCACA GAGAAGGCAC 
CAGAAGGAGA AGTCTGGCCT CATCCCTACC TCGCCAACGC CCTACACTTA CAACAAGAGC 
TGTGACTTGT GGTCCCTCGG GGTGATAATT TATGTGATGC TATGCGGATA CCCTCCTTTT 
TACTCCAAAC ACCACAGTCG GACCATCCCA AAGGACATGC GGAAAAAGAT CATGACAGGG 
AGTTTTGAGT TCCCGGAGGA AGAGTGGAGC CAGATCTCAG AGATGGCCAA AGACGTGGTG 
AGGAAGCTTC TAAAGGTCAA ACCAGAGGAA AGACTCACAA TCGAGGGGGT GTTGGACCAT 
CCCTGGCTCA ACTCGACAGA GGCCCTGGAT AATGTGCTAC CCTCTGCTCA GCTGATGATG 
GATAAGGCAG TGGTTGCAGG GATCCAGCAG GCTCATGCCG AGCAGCTGGC AAACATGAGG 
ATCCAGGACC TCAAAGTCAG CCTCAAACCC CTGCACTCTG TGAACAACCC CATTCTGAGG 
AAGAGGAAGC TACTGGGCTC CAAGCCAAAG GATGGTGTTT ATATCCACGA CCATGAGAAT 
GGAGTCGAGG ATTCAAATGT TGCCTTGGAA AAGCTTCGCG ATGTCATTGC CCAGTGCATC 
CTTCCCCAGG CTGGTAAAGG AGAGAATGAA GATGAGAAGC TCAATGAGGT AATGCAGGAG 
GCCTGGAAGT ACAACCGGGA GTGCAAACTC CTGCGGGACG CGCTGCAGAG TTTTAGCTGG 
AATGGTCGTG GATTCACAGA TAAAGTGGAC CGATTAAAGC TGGCAGAGGT TGTGAAGCAG 
GTGATCGAAG AGCAGACCCT TCCCCACGAG CCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The extracellular signal-regulated kinase 3 (mitogen-activated protein kinase 6 [MAPK6])-MAPK-activated protein kinase 5 signaling complex regulates septin function and dendrite morphology.
Molecular and cellular biology32(13)2467-78(2012 Jul)
Brand F,Schumacher S,Kant S,Menon MB,Simon R,Turgeon B,Britsch S,Meloche S,Gaestel M,Kotlyarov A

High-throughput RNAi screening reveals novel regulators of telomerase.
Cancer research71(9)3328-40(2011 May)
Cerone MA,Burgess DJ,Naceur-Lombardelli C,Lord CJ,Ashworth A

The MK5/PRAK kinase and Myc form a negative feedback loop that is disrupted during colorectal tumorigenesis.
Molecular cell41(4)445-57(2011 Feb)
Kress TR,Cannell IG,Brenkman AB,Samans B,Gaestel M,Roepman P,Burgering BM,Bushell M,Rosenwald A,Eilers M

PRAK is essential for ras-induced senescence and tumor suppression.
Cell128(2)295-308(2007 Jan)
Sun P,Yoshizuka N,New L,Moser BA,Li Y,Liao R,Xie C,Chen J,Deng Q,Yamout M,Dong MQ,Frangou CG,Yates JR 3rd,Wright PE,Han J

MAPKAP kinases - MKs - two's company, three's a crowd.
Nature reviews. Molecular cell biology7(2)120-30(2006 Feb)
Gaestel M

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Mapkapk5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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