Tmem100 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmem100
Entrez Gene ID	497979
Full Name	transmembrane protein 100
General protein information	Preferred Names transmembrane protein 100 Names transmembrane protein 100
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26

mRNA and Protein(s)

mRNA	Protein	Name
XM_008768102.2	XP_008766324.1	transmembrane protein 100 isoform X1
NM_001017479.1	NP_001017479.1	transmembrane protein 100

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TMEM100	XP_003315670.1
Canis lupus familiaris (dog)	TMEM100	XP_005624756.1
Homo sapiens (human)	TMEM100	NP_001093110.1
Mus musculus (house mouse)	Tmem100	NP_080709.1
Gallus gallus (chicken)	TMEM100	XP_415651.1
Danio rerio (zebrafish)	LOC100334742	XP_002667042.1
	TMEM100	XP_001103766.1
Bos taurus (cattle)	TMEM100	NP_001039987.1
Xenopus tropicalis (tropical clawed frog)	tmem100	XP_004918619.1
Rattus norvegicus (Norway rat)	Tmem100	NP_001017479.1

Gene Ontology
Bibliography

Related articles in PubMed

Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain.
Weng HJ, Patel KN, Jeske NA, Bierbower SM, Zou W, Tiwari V, Zheng Q, Tang Z, Mo GC, Wang Y, Geng Y, Zhang J, Guan Y, Akopian AN, Dong X
Neuron85(4)833-46(2015 Feb)

Impairment of endothelial-mesenchymal transformation during atrioventricular cushion formation in Tmem100 null embryos.
Mizuta K, Sakabe M, Hashimoto A, Ioka T, Sakai C, Okumura K, Hattammaru M, Fujita M, Araki M, Somekawa S, Saito Y, Nakagawa O
Developmental dynamics : an official publication of the American Association of Anatomists244(1)31-42(2015 Jan)

Distribution of TMEM100 in the mouse and human gastrointestinal tract--a novel marker of enteric nerves.
Eisenman ST, Gibbons SJ, Singh RD, Bernard CE, Wu J, Sarr MG, Kendrick ML, Larson DW, Dozois EJ, Shen KR, Farrugia G
Neuroscience240117-28(2013 Jun)

Tmem100, an ALK1 receptor signaling-dependent gene essential for arterial endothelium differentiation and vascular morphogenesis.
Somekawa S, Imagawa K, Hayashi H, Sakabe M, Ioka T, Sato GE, Inada K, Iwamoto T, Mori T, Uemura S, Nakagawa O, Saito Y
Proceedings of the National Academy of Sciences of the United States of America109(30)12064-9(2012 Jul)

Generation of mice with a conditional and reporter allele for Tmem100.
Moon EH, Kim MJ, Ko KS, Kim YS, Seo J, Oh SP, Lee YJ
Genesis (New York, N.Y. : 2000)48(11)673-8(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmem100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmem100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05949	NM_001017479.1 Latest version!	Rattus norvegicus transmembrane protein 100 (Tmem100), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa05949	XM_008768102.2 Latest version!	Rattus norvegicus transmembrane protein 100 (Tmem100), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmem100 ( NM_001017479.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001017479.1 Rattus norvegicus transmembrane protein 100 (Tmem100), mRNA.

Price & Availability↑

CloneID	ORa05949
Clone ID Related Accession (Same CDS sequence)	XM_008768102.2 , NM_001017479.1
Accession Version	NM_001017479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane protein 100
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092577.1. On Apr 29, 2005 this sequence version replaced XM_573175.1. ##Evidence-Data-START## Transcript exon combination :: BC092577.1, FQ212975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGACTGAAG AACCCACAAA AGAGAACCTG GGAGGCCCGA AGTCTCCCAC ACCTGTGACA 
ATGGAGAAAA GCCCCAAGAG TGAAGTTGTG GTCACCACGG TCCCCTTGGT CAGTGAGGTT 
CAGCTGACGG CCGCCACCGG GGGTGCCGAA CTCTCTTGCT ACCGCTGCAT CATCCCCTTT 
GCCGTGGTGG TCTTCATCAC CGGGATCGTG GTCACCGCTG TAGCTTACAG CTTCAATTCC 
CATGGTTCCG TCATCTCCAT CTTAGGCCTG GTCCTTCTGT CCTCTGGACT GTTTTTACTA 
GCCTCCAGCG CGTTGTGCTG GAAGGTGAGA CAAAGGAACA AAAAAGTCAA GAGACGCGAG 
AGTCAGACGG CTCTGGTGGT AAATCAGAGA AGTTTGTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017479.1
CDS	233..637
Translation	MTEEPTKENLGGPKSPTPVTMEKSPKSEVVVTTVPLVSEVQLTAATGGAELSCYRCIIPFAVVVFITGIVVTAVAYSFNSHGSVISILGLVLLSSGLFLLASSALCWKVRQRNKKVKRRESQTALVVNQRSLFA

Target ORF information:

RefSeq Version	NM_001017479.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane protein 100 (Tmem100), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017479.1

ORF Insert Sequence:

ATGACTGAAG AACCCACAAA AGAGAACCTG GGAGGCCCGA AGTCTCCCAC ACCTGTGACA 
ATGGAGAAAA GCCCCAAGAG TGAAGTTGTG GTCACCACGG TCCCCTTGGT CAGTGAGGTT 
CAGCTGACGG CCGCCACCGG GGGTGCCGAA CTCTCTTGCT ACCGCTGCAT CATCCCCTTT 
GCCGTGGTGG TCTTCATCAC CGGGATCGTG GTCACCGCTG TAGCTTACAG CTTCAATTCC 
CATGGTTCCG TCATCTCCAT CTTAGGCCTG GTCCTTCTGT CCTCTGGACT GTTTTTACTA 
GCCTCCAGCG CGTTGTGCTG GAAGGTGAGA CAAAGGAACA AAAAAGTCAA GAGACGCGAG 
AGTCAGACGG CTCTGGTGGT AAATCAGAGA AGTTTGTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmem100 ( XM_008768102.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766324.1 Rattus norvegicus transmembrane protein 100 (Tmem100), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05949
Clone ID Related Accession (Same CDS sequence)	XM_008768102.2 , NM_001017479.1
Accession Version	XM_008768102.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane protein 100 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768102.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAAG AACCCACAAA AGAGAACCTG GGAGGCCCGA AGTCTCCCAC ACCTGTGACA 
ATGGAGAAAA GCCCCAAGAG TGAAGTTGTG GTCACCACGG TCCCCTTGGT CAGTGAGGTT 
CAGCTGACGG CCGCCACCGG GGGTGCCGAA CTCTCTTGCT ACCGCTGCAT CATCCCCTTT 
GCCGTGGTGG TCTTCATCAC CGGGATCGTG GTCACCGCTG TAGCTTACAG CTTCAATTCC 
CATGGTTCCG TCATCTCCAT CTTAGGCCTG GTCCTTCTGT CCTCTGGACT GTTTTTACTA 
GCCTCCAGCG CGTTGTGCTG GAAGGTGAGA CAAAGGAACA AAAAAGTCAA GAGACGCGAG 
AGTCAGACGG CTCTGGTGGT AAATCAGAGA AGTTTGTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766324.1
CDS	457..861
Translation	MTEEPTKENLGGPKSPTPVTMEKSPKSEVVVTTVPLVSEVQLTAATGGAELSCYRCIIPFAVVVFITGIVVTAVAYSFNSHGSVISILGLVLLSSGLFLLASSALCWKVRQRNKKVKRRESQTALVVNQRSLFA

Target ORF information:

RefSeq Version	XM_008768102.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane protein 100 (Tmem100), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768102.2

ORF Insert Sequence:

ATGACTGAAG AACCCACAAA AGAGAACCTG GGAGGCCCGA AGTCTCCCAC ACCTGTGACA 
ATGGAGAAAA GCCCCAAGAG TGAAGTTGTG GTCACCACGG TCCCCTTGGT CAGTGAGGTT 
CAGCTGACGG CCGCCACCGG GGGTGCCGAA CTCTCTTGCT ACCGCTGCAT CATCCCCTTT 
GCCGTGGTGG TCTTCATCAC CGGGATCGTG GTCACCGCTG TAGCTTACAG CTTCAATTCC 
CATGGTTCCG TCATCTCCAT CTTAGGCCTG GTCCTTCTGT CCTCTGGACT GTTTTTACTA 
GCCTCCAGCG CGTTGTGCTG GAAGGTGAGA CAAAGGAACA AAAAAGTCAA GAGACGCGAG 
AGTCAGACGG CTCTGGTGGT AAATCAGAGA AGTTTGTTTG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain.
Neuron85(4)833-46(2015 Feb)
Weng HJ,Patel KN,Jeske NA,Bierbower SM,Zou W,Tiwari V,Zheng Q,Tang Z,Mo GC,Wang Y,Geng Y,Zhang J,Guan Y,Akopian AN,Dong X

Impairment of endothelial-mesenchymal transformation during atrioventricular cushion formation in Tmem100 null embryos.
Developmental dynamics : an official publication of the American Association of Anatomists244(1)31-42(2015 Jan)
Mizuta K,Sakabe M,Hashimoto A,Ioka T,Sakai C,Okumura K,Hattammaru M,Fujita M,Araki M,Somekawa S,Saito Y,Nakagawa O

Distribution of TMEM100 in the mouse and human gastrointestinal tract--a novel marker of enteric nerves.
Neuroscience240117-28(2013 Jun)
Eisenman ST,Gibbons SJ,Singh RD,Bernard CE,Wu J,Sarr MG,Kendrick ML,Larson DW,Dozois EJ,Shen KR,Farrugia G

Tmem100, an ALK1 receptor signaling-dependent gene essential for arterial endothelium differentiation and vascular morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America109(30)12064-9(2012 Jul)
Somekawa S,Imagawa K,Hayashi H,Sakabe M,Ioka T,Sato GE,Inada K,Iwamoto T,Mori T,Uemura S,Nakagawa O,Saito Y

Generation of mice with a conditional and reporter allele for Tmem100.
Genesis (New York, N.Y. : 2000)48(11)673-8(2010 Nov)
Moon EH,Kim MJ,Ko KS,Kim YS,Seo J,Oh SP,Lee YJ

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Tmem100 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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