Si cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Si
Entrez Gene ID	497756
Full Name	sucrase-isomaltase
Synonyms	SUCIMAL
General protein information	Preferred Names sucrase-isomaltase Names sucrase-isomaltase, intestinal sucrase-isomaltase (alpha-glucosidase)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q32
Summary	catalyzes the hydrolysis of sucrose and maltose [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013061.1	NP_037193.1	sucrase-isomaltase, intestinal

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SI	XP_545265.3
Mus musculus (house mouse)	Sis	NP_001074606.1
Homo sapiens (human)	SI	NP_001032.2
	SI	XP_002802979.1
Bos taurus (cattle)	SI	NP_001107661.1
Pan troglodytes (chimpanzee)	SI	XP_001159286.2
Rattus norvegicus (Norway rat)	Si	NP_037193.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains.
Sim L, Willemsma C, Mohan S, Naim HY, Pinto BM, Rose DR
The Journal of biological chemistry285(23)17763-70(2010 Jun)

Induction of histone acetylation on the sucrase-isomaltase gene in the postnatal rat jejunum.
Yorita S, Mochizuki K, Goda T
Bioscience, biotechnology, and biochemistry73(4)933-5(2009 Apr)

Sodium tungstate decreases sucrase and Na+/D-glucose cotransporter in the jejunum of diabetic rats.
Mir?-Queralt M, Guinovart JJ, Planas JM
American journal of physiology. Gastrointestinal and liver physiology295(3)G479-84(2008 Sep)

Histone H3 modifications and Cdx-2 binding to the sucrase-isomaltase (SI) gene is involved in induction of the gene in the transition from the crypt to villus in the small intestine of rats.
Suzuki T, Mochizuki K, Goda T
Biochemical and biophysical research communications369(2)788-93(2008 May)

Effect of harmaline on rat intestinal brush border sucrase activity.
Kaur N, Kaur J, Mahmood A
Indian journal of biochemistry & biophysics39(2)119-23(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

An abrupt jejunal induction of histone acetylation changes on the sucrase-isomaltase gene during this period may be concerned with the expression of the gene.
Title: Induction of histone acetylation on the sucrase-isomaltase gene in the postnatal rat jejunum.

The antidiabetic effect of tungstate is partly due to normalization of the activity of sucrase and SGLT1 in the brush-border membrane of enterocytes.
Title: Sodium tungstate decreases sucrase and Na+/D-glucose cotransporter in the jejunum of diabetic rats.

These results suggest that induction of the SI gene during the transition from the crypt to the villi is associated with changes in histone H3 modifications from methylation at lysine 9 to di-acetylation at lysine 9/14.
Title: Histone H3 modifications and Cdx-2 binding to the sucrase-isomaltase (SI) gene is involved in induction of the gene in the transition from the crypt to villus in the small intestine of rats.

Spermidine induction of sucrase-isomaltase in small intestine may be mediated by altered ornithine decarboxylase expression.
Title: Oral polyamine administration modifies the ontogeny of hexose transporter gene expression in the postnatal rat intestine.

harmaline has an effect on rat intestinal brush border sucrase activity
Title: Effect of harmaline on rat intestinal brush border sucrase activity.

The following Si gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Si cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01821	NM_013061.1 Latest version!	Rattus norvegicus sucrase-isomaltase (Si), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Si ( NM_013061.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037193.1 Rattus norvegicus sucrase-isomaltase (Si), mRNA.

Price & Availability↑

CloneID	ORa01821
Clone ID Related Accession (Same CDS sequence)	NM_013061.1
Accession Version	NM_013061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	sucrase-isomaltase, intestinal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L25926.1. On Jan 11, 2004 this sequence version replaced XM_346624.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L25926.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN02642794 [ECO:0000350] ##Evidence-Data-END##

ATGGCAAAGA AGAAGTTCAG TGCACTAGAG ATCTCTCTGA TTGTCCTCTT CATCATAGTA 
ACAGCAATAG CTATTGCTCT TGTCACTGTT TTAGCAACCA AGGTGCCAGC TGTGGAAGAA 
ATAAAAAGTC CAACTCCAAC TTCAAATTCA ACTCCAACTT CAACTCCAAC TTCAACTTCA 
ACCCCAACTT CAACTTCAAC CCCTAGTCCT GGGAAGTGTC CTCCCGAACA AGGTGAACCT 
ATCAATGAGA GAATTAACTG GATTCCAGAA CAACATCCAA CAAAGGCAAT ATGTGAAGAG 
CGTGGCTGTT GCTGGCGTCC GTGGAACAAC ACTGTCATTC CTTGGTGCTT CTTTGCAGAC 
AACCATGGCT ATAATGCTGA GTCCATCACA AATGAAAACG CGGGACTTAA AGCCACACTG 
AACAGGATCC CTTCTCCCAC TCTATTTGGA GAAGACATTA AAAGCGTCAT CCTCACAACT 
CAGACTCAAA CAGGCAATCG TTTCCGGTTC AAGATCACAG ATCCAAATAA CAAGAGATAT 
GAAGTGCCGC ACCAGTTTGT AAAAGAGGAA ACTGGAATCC CAGCAGCTGA CACACTCTAT 
GATGTCCAGG TCTCAGAAAA TCCGTTCAGC ATCAAAGTGA TCAGGAAAAG CAACAACAAG 
GTCTTGTGTG ATACCAGTGT TGGTCCCCTG TTGTACTCAA ACCAATATTT GCAGATTTCG 
ACAAGACTTC CAAGTGAATA TATTTATGGC TTTGGGGGAC ATATTCATAA GAGATTTCGT 
CATGACTTAT ACTGGAAGAC ATGGCCAATC TTTACTCGAG ATGAAATCCC TGGTGATAAT 
AATCATAATC TCTATGGGCA TCAGACATTC TTCATGGGCA TTGGAGATAC CTCTGGGAAG 
TCATATGGTG TTTTCCTTAT GAACAGCAAT GCAATGGAGG TTTTCATCCA GCCAACTCCA 
ATAATTACTT ACAGAGTTAC TGGTGGCATA CTAGACTTTT ATATATTTCT TGGAGACACA 
CCAGAACAAG TAGTTCAACA ATACCAAGAG GTTCATTGGC GACCGGCGAT GCCAGCATAT 
TGGAACCTTG GCTTTCAACT GAGTCGCTGG AATTACGGGT CATTAGACAC TGTGTCAGAA 
GTAGTAAGAA GAAACCGGGA AGCTGGCATC CCATATGATG CACAAGTCAC TGATATTGAC 
TATATGGAAG ACCACAAGGA ATTCACTTAT GATAGAGTCA AATTTAATGG ACTTCCTGAA 
TTTGCCCAGG ATTTACATAA CCATGGCAAA TATATTATCA TCCTGGACCC TGCAATCTCC 
ATAAATAAGC GAGCCAATGG TGCAGAATAT CAAACATATG TGAGAGGGAA TGAGAAAAAT 
GTTTGGGTGA ATGAGTCAGA TGGGACTACA CCACTTATTG GAGAGGTATG GCCAGGATTA 
ACAGTGTACC CAGATTTTAC TAATCCCCAA ACCATCGAGT GGTGGGCAAA TGAATGCAAT 
CTTTTCCATC AGCAAGTCGA ATATGATGGA CTATGGATTG ACATGAATGA AGTTTCCAGC 
TTTATCCAAG GGTCTCTGAA TCTGAAGGGT GTACTTCTAA TCGTGCTGAA CTACCCTCCT 
TTTACTCCTG GTATTCTTGA CAAAGTCATG TATTCCAAAA CTCTTTGCAT GGATGCTGTG 
CAGCACTGGG GGAAGCAGTA TGATGTACAT AGCCTGTATG GGTACAGCAT GGCTATAGCC 
ACAGAGCAAG CTGTGGAAAG AGTTTTTCCT AATAAGAGAA GCTTCATCCT CACCCGGTCA 
ACCTTTGGTG GCTCTGGAAG GCATGCTAAT CATTGGCTCG GAGACAACAC AGCCTCGTGG 
GAGCAGATGG AATGGTCCAT CACAGGAATG CTGGAATTTG GCATATTTGG CATGCCTTTG 
GTAGGCGCGA CATCTTGTGG ATTTTTGGCT GACACCACAG AAGAACTCTG CAGAAGATGG 
ATGCAACTGG GAGCCTTTTA CCCATTTTCT AGAAACCACA ATGCAGAAGG CTACATGGAA 
CAGGATCCTG CTTATTTCGG ACAAGACTCA TCAAGGCACT ACCTAACCAT CCGCTATACT 
TTACTCCCTT TCCTCTACAC TCTGTTCTAT AGAGCCCACA TGTTTGGTGA GACAGTCGCA 
AGGCCATTTC TTTATGAGTT TTACGATGAC ACTAACAGCT GGATTGAGGA CACCCAGTTT 
CTGTGGGGCC CTGCACTACT TATCACTCCT GTTTTGAGAC CGGGAGTAGA AAACGTGAGT 
GCATATATTC CTAATGCTAC CTGGTATGAT TATGAAACAG GTATAAAAAG GCCATGGAGG 
AAAGAAAGGA TTAACATGTA CCTGCCAGGA GATAAAATAG GATTACATCT TAGAGGAGGT 
TATATCATCC CTACCCAGGA ACCTGATGTA ACAACAACAG CAAGCCGAAA GAATCCTCTA 
GGTCTCATTG TTGCATTAGA TGATAATCAG GCAGCAAAAG GAGAATTATT CTGGGATGAT 
GGCGAATCTA AAGATTCCAT AGAAAAGAAA ATGTATATAT TATACACGTT TTCAGTCTCA 
AATAATGAAT TGGTTCTAAA CTGCACGCAT TCATCATATG CAGAGGGAAC TTCCCTTGCA 
TTTAAAACAA TCAAAGTCCT TGGGCTGCGA GAGGATGTCA GGAGCATTAC AGTGGGTGAA 
AATGACCAGC AAATGGCTAC TCATACTAAC TTCACTTTTG ATTCTGCCAA TAAGATTCTG 
TCAATTACAG CTCTCAACTT CAACCTTGCC GGAAGCTTCA TAGTTAGATG GTGCCGAACC 
TTCTCAGACA ATGAGAAATT CACCTGTTAC CCAGATGTAG GCACAGCGAC AGAGGGGACA 
TGCACACAGA GAGGCTGCTT ATGGCAACCG GTTAGTGGAC TCTCCAATGT GCCTCCATAC 
TACTTTCCTC CAGAAAACAA TCCCTATACG TTAACTTCAA TTCAACCTTT GCCCACGGGC 
ATAACAGCAG AACTCCAGTT GAATCCTCCA AATGCTAGAA TCAAGCTTCC ATCTAATCCC 
ATCTCCACTC TCCGTGTGGG AGTAAAATAT CACCCAAATG ACATGCTACA GTTTAAAATT 
TATGATGCTC AGCATAAGAG ATATGAAGTT CCTGTACCAC TAAATATTCC AGACACCCCT 
ACCAGTTCTA ATGAAAGACT CTATGATGTA GAAATCAAGG AAAATCCTTT TGGCATCCAG 
GTTCGAAGAA GAAGCAGTGG AAAGCTTATC TGGGATTCTA GACTGCCTGG ATTTGGCTTC 
AATGATCAGT TTATCCAGAT ATCTACACGT CTGCCATCAA ATTATTTATA TGGTTTCGGG 
GAAGTGGAAC ACACGGCATT CAAGCGCGAT TTGAATTGGC ACACTTGGGG AATGTTCACA 
AGAGATCAAC CTCCTGGATA TAAACTTAAT TCCTATGGAT TTCATCCCTA TTATATGGCT 
TTGGAGAATG AGGGCAATGC TCATGGAGTT CTGTTACTGA ACAGCAATGG AATGGATGTT 
ACATTCCAGC CAACTCCTGC TCTAACATAC CGCACAATTG GTGGAATCTT GGATTTTTAC 
ATGTTTTTGG GACCAACACC AGAAATTGCA ACAAGACAAT ATCATGAAGT AATTGGCTTT 
CCAGTCATGC CACCTTACTG GGCTCTAGGA TTCCAATTAT GTCGTTATGG ATACAGAAAT 
ACTTCAGAGA TTGAACAACT ATATAACGAC ATGGTGGCTG CTAATATCCC CTATGATGTT 
CAGTATACAG ACATCAATTA TATGGAAAGG CAACTAGACT TCACAATTGG TGAACGATTC 
AAGACTCTTC CTGAATTTGT TGACCGAATA AGAAAAGATG GAATGAAATA TATTGTTATA 
CTGGCTCCAG CAATTTCAGG AAATGAAACA CAGCCTTACC CTGCCTTTGA GCGGGGAATA 
CAGAAAGATG TTTTTGTCAA ATGGCCTAAC ACAAATGACA TTTGTTGGCC AAAGGTCTGG 
CCAGATTTAC CTAATGTTAC AATAGATGAG ACTATAACCG AAGATGAAGC TGTTAACGCT 
TCCAGAGCAC ATGTAGCATT TCCAGACTTC TTTAGGAATT CCACATTGGA GTGGTGGGCA 
AGAGAAATTT ATGATTTCTA CAATGAAAAA ATGAAGTTTG ATGGCTTGTG GATTGATATG 
AATGAGCCTT CTAGTTTTGG AATTCAGATG GGGGGCAAGG TCCTTAATGA ATGCAGGAGA 
ATGATGACCC TAAATTATCC CCCCGTATTT TCCCCAGAAC TCCGCGTAAA AGAGGGTGAG 
GGGGCTAGCA TTTCCGAAGC CATGTGTATG GAGACTGAAC ATATCCTTAT CGATGGCTCA 
TCAGTTTTGC AGTATGATGT TCACAATTTA TATGGGTGGT CTCAAGTAAA ACCCACTCTG 
GATGCATTAC AGAACACCAC TGGTCTGAGA GGGATAGTCA TCTCTCGGTC CACATATCCC 
ACAACTGGTC GATGGGGTGG ACACTGGCTT GGAGACAATT ATACCACCTG GGATAATTTG 
GAAAAATCAC TCATTGGCAT GCTGGAACTT AATCTTTTTG GGATACCATA CATTGGAGCA 
GATATCTGCG GCGTTTTTCA TGATTCAGGA TACCCATCTC TGTACTTCGT TGGGATCCAA 
GTTGGAGCCT TTTATCCATA TCCAAGGGAA TCCCCAACAA TCAATTTTAC TAGAAGCCAA 
GATCCTGTTT CCTGGATGAA ACTTTTGCTC CAAATGTCAA AGAAAGTTCT AGAAATCAGA 
TACACTTTGC TGCCCTATTT CTACACACAG ATGCATGAAG CCCATGCTCA TGGTGGAACT 
GTCATCAGAC CCCTCATGCA TGAGTTCTTT GATGATAAAG AAACCTGGGA AATATACAAG 
CAGTTCCTAT GGGGTCCAGC TTTTATGGTG ACTCCTGTCG TTGAACCTTT CAGGACCAGT 
GTGACTGGCT ATGTTCCTAA GGCTCGATGG TTTGACTACC ATACAGGGGC CGATATAAAG 
CTCAAAGGAA TACTTCACAC ATTTTCAGCT CCCTTTGATA CAATAAATCT ACACGTCCGA 
GGAGGTTACA TTCTACCATG TCAAGAGCCA GCACGGAACA CGCATCTAAG TCGACAGAAT 
TACATGAAGC TCATTGTTGC TGCAGATGAC AACCAAATGG CACAAGGGAC TCTATTTGGG 
GATGATGGAG AGTCTATAGA CACTTATGAA AGAGGTCAAT ACACCTCGAT ACAATTCAAT 
TTAAATCAGA CAACTTTAAC AAGCACTGTA TTGGCAAATG GGTATAAAAA CAAACAGGAG 
ATGAGACTTG GCAGCATCCA CATATGGGGG AAAGGAACAC TCCGTATCAG CAATGCTAAT 
TTGGTTTATG GAGGCCGTAA ACATCAGCCT CCTTTTACTC AAGAGGAAGC TAAGGAGACA 
CTAATATTTG ACCTGAAAAA TATGAATGTT ACTCTTGATG AACCAATACA AATCACCTGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037193.1
CDS	42..5567
Translation	MAKKKFSALEISLIVLFIIVTAIAIALVTVLATKVPAVEEIKSPTPTSNSTPTSTPTSTSTPTSTSTPSPGKCPPEQGEPINERINWIPEQHPTKAICEERGCCWRPWNNTVIPWCFFADNHGYNAESITNENAGLKATLNRIPSPTLFGEDIKSVILTTQTQTGNRFRFKITDPNNKRYEVPHQFVKEETGIPAADTLYDVQVSENPFSIKVIRKSNNKVLCDTSVGPLLYSNQYLQISTRLPSEYIYGFGGHIHKRFRHDLYWKTWPIFTRDEIPGDNNHNLYGHQTFFMGIGDTSGKSYGVFLMNSNAMEVFIQPTPIITYRVTGGILDFYIFLGDTPEQVVQQYQEVHWRPAMPAYWNLGFQLSRWNYGSLDTVSEVVRRNREAGIPYDAQVTDIDYMEDHKEFTYDRVKFNGLPEFAQDLHNHGKYIIILDPAISINKRANGAEYQTYVRGNEKNVWVNESDGTTPLIGEVWPGLTVYPDFTNPQTIEWWANECNLFHQQVEYDGLWIDMNEVSSFIQGSLNLKGVLLIVLNYPPFTPGILDKVMYSKTLCMDAVQHWGKQYDVHSLYGYSMAIATEQAVERVFPNKRSFILTRSTFGGSGRHANHWLGDNTASWEQMEWSITGMLEFGIFGMPLVGATSCGFLADTTEELCRRWMQLGAFYPFSRNHNAEGYMEQDPAYFGQDSSRHYLTIRYTLLPFLYTLFYRAHMFGETVARPFLYEFYDDTNSWIEDTQFLWGPALLITPVLRPGVENVSAYIPNATWYDYETGIKRPWRKERINMYLPGDKIGLHLRGGYIIPTQEPDVTTTASRKNPLGLIVALDDNQAAKGELFWDDGESKDSIEKKMYILYTFSVSNNELVLNCTHSSYAEGTSLAFKTIKVLGLREDVRSITVGENDQQMATHTNFTFDSANKILSITALNFNLAGSFIVRWCRTFSDNEKFTCYPDVGTATEGTCTQRGCLWQPVSGLSNVPPYYFPPENNPYTLTSIQPLPTGITAELQLNPPNARIKLPSNPISTLRVGVKYHPNDMLQFKIYDAQHKRYEVPVPLNIPDTPTSSNERLYDVEIKENPFGIQVRRRSSGKLIWDSRLPGFGFNDQFIQISTRLPSNYLYGFGEVEHTAFKRDLNWHTWGMFTRDQPPGYKLNSYGFHPYYMALENEGNAHGVLLLNSNGMDVTFQPTPALTYRTIGGILDFYMFLGPTPEIATRQYHEVIGFPVMPPYWALGFQLCRYGYRNTSEIEQLYNDMVAANIPYDVQYTDINYMERQLDFTIGERFKTLPEFVDRIRKDGMKYIVILAPAISGNETQPYPAFERGIQKDVFVKWPNTNDICWPKVWPDLPNVTIDETITEDEAVNASRAHVAFPDFFRNSTLEWWAREIYDFYNEKMKFDGLWIDMNEPSSFGIQMGGKVLNECRRMMTLNYPPVFSPELRVKEGEGASISEAMCMETEHILIDGSSVLQYDVHNLYGWSQVKPTLDALQNTTGLRGIVISRSTYPTTGRWGGHWLGDNYTTWDNLEKSLIGMLELNLFGIPYIGADICGVFHDSGYPSLYFVGIQVGAFYPYPRESPTINFTRSQDPVSWMKLLLQMSKKVLEIRYTLLPYFYTQMHEAHAHGGTVIRPLMHEFFDDKETWEIYKQFLWGPAFMVTPVVEPFRTSVTGYVPKARWFDYHTGADIKLKGILHTFSAPFDTINLHVRGGYILPCQEPARNTHLSRQNYMKLIVAADDNQMAQGTLFGDDGESIDTYERGQYTSIQFNLNQTTLTSTVLANGYKNKQEMRLGSIHIWGKGTLRISNANLVYGGRKHQPPFTQEEAKETLIFDLKNMNVTLDEPIQITWS

Target ORF information:

RefSeq Version	NM_013061.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sucrase-isomaltase (Si), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013061.1

ORF Insert Sequence:

ATGGCAAAGA AGAAGTTCAG TGCACTAGAG ATCTCTCTGA TTGTCCTCTT CATCATAGTA 
ACAGCAATAG CTATTGCTCT TGTCACTGTT TTAGCAACCA AGGTGCCAGC TGTGGAAGAA 
ATAAAAAGTC CAACTCCAAC TTCAAATTCA ACTCCAACTT CAACTCCAAC TTCAACTTCA 
ACCCCAACTT CAACTTCAAC CCCTAGTCCT GGGAAGTGTC CTCCCGAACA AGGTGAACCT 
ATCAATGAGA GAATTAACTG GATTCCAGAA CAACATCCAA CAAAGGCAAT ATGTGAAGAG 
CGTGGCTGTT GCTGGCGTCC GTGGAACAAC ACTGTCATTC CTTGGTGCTT CTTTGCAGAC 
AACCATGGCT ATAATGCTGA GTCCATCACA AATGAAAACG CGGGACTTAA AGCCACACTG 
AACAGGATCC CTTCTCCCAC TCTATTTGGA GAAGACATTA AAAGCGTCAT CCTCACAACT 
CAGACTCAAA CAGGCAATCG TTTCCGGTTC AAGATCACAG ATCCAAATAA CAAGAGATAT 
GAAGTGCCGC ACCAGTTTGT AAAAGAGGAA ACTGGAATCC CAGCAGCTGA CACACTCTAT 
GATGTCCAGG TCTCAGAAAA TCCGTTCAGC ATCAAAGTGA TCAGGAAAAG CAACAACAAG 
GTCTTGTGTG ATACCAGTGT TGGTCCCCTG TTGTACTCAA ACCAATATTT GCAGATTTCG 
ACAAGACTTC CAAGTGAATA TATTTATGGC TTTGGGGGAC ATATTCATAA GAGATTTCGT 
CATGACTTAT ACTGGAAGAC ATGGCCAATC TTTACTCGAG ATGAAATCCC TGGTGATAAT 
AATCATAATC TCTATGGGCA TCAGACATTC TTCATGGGCA TTGGAGATAC CTCTGGGAAG 
TCATATGGTG TTTTCCTTAT GAACAGCAAT GCAATGGAGG TTTTCATCCA GCCAACTCCA 
ATAATTACTT ACAGAGTTAC TGGTGGCATA CTAGACTTTT ATATATTTCT TGGAGACACA 
CCAGAACAAG TAGTTCAACA ATACCAAGAG GTTCATTGGC GACCGGCGAT GCCAGCATAT 
TGGAACCTTG GCTTTCAACT GAGTCGCTGG AATTACGGGT CATTAGACAC TGTGTCAGAA 
GTAGTAAGAA GAAACCGGGA AGCTGGCATC CCATATGATG CACAAGTCAC TGATATTGAC 
TATATGGAAG ACCACAAGGA ATTCACTTAT GATAGAGTCA AATTTAATGG ACTTCCTGAA 
TTTGCCCAGG ATTTACATAA CCATGGCAAA TATATTATCA TCCTGGACCC TGCAATCTCC 
ATAAATAAGC GAGCCAATGG TGCAGAATAT CAAACATATG TGAGAGGGAA TGAGAAAAAT 
GTTTGGGTGA ATGAGTCAGA TGGGACTACA CCACTTATTG GAGAGGTATG GCCAGGATTA 
ACAGTGTACC CAGATTTTAC TAATCCCCAA ACCATCGAGT GGTGGGCAAA TGAATGCAAT 
CTTTTCCATC AGCAAGTCGA ATATGATGGA CTATGGATTG ACATGAATGA AGTTTCCAGC 
TTTATCCAAG GGTCTCTGAA TCTGAAGGGT GTACTTCTAA TCGTGCTGAA CTACCCTCCT 
TTTACTCCTG GTATTCTTGA CAAAGTCATG TATTCCAAAA CTCTTTGCAT GGATGCTGTG 
CAGCACTGGG GGAAGCAGTA TGATGTACAT AGCCTGTATG GGTACAGCAT GGCTATAGCC 
ACAGAGCAAG CTGTGGAAAG AGTTTTTCCT AATAAGAGAA GCTTCATCCT CACCCGGTCA 
ACCTTTGGTG GCTCTGGAAG GCATGCTAAT CATTGGCTCG GAGACAACAC AGCCTCGTGG 
GAGCAGATGG AATGGTCCAT CACAGGAATG CTGGAATTTG GCATATTTGG CATGCCTTTG 
GTAGGCGCGA CATCTTGTGG ATTTTTGGCT GACACCACAG AAGAACTCTG CAGAAGATGG 
ATGCAACTGG GAGCCTTTTA CCCATTTTCT AGAAACCACA ATGCAGAAGG CTACATGGAA 
CAGGATCCTG CTTATTTCGG ACAAGACTCA TCAAGGCACT ACCTAACCAT CCGCTATACT 
TTACTCCCTT TCCTCTACAC TCTGTTCTAT AGAGCCCACA TGTTTGGTGA GACAGTCGCA 
AGGCCATTTC TTTATGAGTT TTACGATGAC ACTAACAGCT GGATTGAGGA CACCCAGTTT 
CTGTGGGGCC CTGCACTACT TATCACTCCT GTTTTGAGAC CGGGAGTAGA AAACGTGAGT 
GCATATATTC CTAATGCTAC CTGGTATGAT TATGAAACAG GTATAAAAAG GCCATGGAGG 
AAAGAAAGGA TTAACATGTA CCTGCCAGGA GATAAAATAG GATTACATCT TAGAGGAGGT 
TATATCATCC CTACCCAGGA ACCTGATGTA ACAACAACAG CAAGCCGAAA GAATCCTCTA 
GGTCTCATTG TTGCATTAGA TGATAATCAG GCAGCAAAAG GAGAATTATT CTGGGATGAT 
GGCGAATCTA AAGATTCCAT AGAAAAGAAA ATGTATATAT TATACACGTT TTCAGTCTCA 
AATAATGAAT TGGTTCTAAA CTGCACGCAT TCATCATATG CAGAGGGAAC TTCCCTTGCA 
TTTAAAACAA TCAAAGTCCT TGGGCTGCGA GAGGATGTCA GGAGCATTAC AGTGGGTGAA 
AATGACCAGC AAATGGCTAC TCATACTAAC TTCACTTTTG ATTCTGCCAA TAAGATTCTG 
TCAATTACAG CTCTCAACTT CAACCTTGCC GGAAGCTTCA TAGTTAGATG GTGCCGAACC 
TTCTCAGACA ATGAGAAATT CACCTGTTAC CCAGATGTAG GCACAGCGAC AGAGGGGACA 
TGCACACAGA GAGGCTGCTT ATGGCAACCG GTTAGTGGAC TCTCCAATGT GCCTCCATAC 
TACTTTCCTC CAGAAAACAA TCCCTATACG TTAACTTCAA TTCAACCTTT GCCCACGGGC 
ATAACAGCAG AACTCCAGTT GAATCCTCCA AATGCTAGAA TCAAGCTTCC ATCTAATCCC 
ATCTCCACTC TCCGTGTGGG AGTAAAATAT CACCCAAATG ACATGCTACA GTTTAAAATT 
TATGATGCTC AGCATAAGAG ATATGAAGTT CCTGTACCAC TAAATATTCC AGACACCCCT 
ACCAGTTCTA ATGAAAGACT CTATGATGTA GAAATCAAGG AAAATCCTTT TGGCATCCAG 
GTTCGAAGAA GAAGCAGTGG AAAGCTTATC TGGGATTCTA GACTGCCTGG ATTTGGCTTC 
AATGATCAGT TTATCCAGAT ATCTACACGT CTGCCATCAA ATTATTTATA TGGTTTCGGG 
GAAGTGGAAC ACACGGCATT CAAGCGCGAT TTGAATTGGC ACACTTGGGG AATGTTCACA 
AGAGATCAAC CTCCTGGATA TAAACTTAAT TCCTATGGAT TTCATCCCTA TTATATGGCT 
TTGGAGAATG AGGGCAATGC TCATGGAGTT CTGTTACTGA ACAGCAATGG AATGGATGTT 
ACATTCCAGC CAACTCCTGC TCTAACATAC CGCACAATTG GTGGAATCTT GGATTTTTAC 
ATGTTTTTGG GACCAACACC AGAAATTGCA ACAAGACAAT ATCATGAAGT AATTGGCTTT 
CCAGTCATGC CACCTTACTG GGCTCTAGGA TTCCAATTAT GTCGTTATGG ATACAGAAAT 
ACTTCAGAGA TTGAACAACT ATATAACGAC ATGGTGGCTG CTAATATCCC CTATGATGTT 
CAGTATACAG ACATCAATTA TATGGAAAGG CAACTAGACT TCACAATTGG TGAACGATTC 
AAGACTCTTC CTGAATTTGT TGACCGAATA AGAAAAGATG GAATGAAATA TATTGTTATA 
CTGGCTCCAG CAATTTCAGG AAATGAAACA CAGCCTTACC CTGCCTTTGA GCGGGGAATA 
CAGAAAGATG TTTTTGTCAA ATGGCCTAAC ACAAATGACA TTTGTTGGCC AAAGGTCTGG 
CCAGATTTAC CTAATGTTAC AATAGATGAG ACTATAACCG AAGATGAAGC TGTTAACGCT 
TCCAGAGCAC ATGTAGCATT TCCAGACTTC TTTAGGAATT CCACATTGGA GTGGTGGGCA 
AGAGAAATTT ATGATTTCTA CAATGAAAAA ATGAAGTTTG ATGGCTTGTG GATTGATATG 
AATGAGCCTT CTAGTTTTGG AATTCAGATG GGGGGCAAGG TCCTTAATGA ATGCAGGAGA 
ATGATGACCC TAAATTATCC CCCCGTATTT TCCCCAGAAC TCCGCGTAAA AGAGGGTGAG 
GGGGCTAGCA TTTCCGAAGC CATGTGTATG GAGACTGAAC ATATCCTTAT CGATGGCTCA 
TCAGTTTTGC AGTATGATGT TCACAATTTA TATGGGTGGT CTCAAGTAAA ACCCACTCTG 
GATGCATTAC AGAACACCAC TGGTCTGAGA GGGATAGTCA TCTCTCGGTC CACATATCCC 
ACAACTGGTC GATGGGGTGG ACACTGGCTT GGAGACAATT ATACCACCTG GGATAATTTG 
GAAAAATCAC TCATTGGCAT GCTGGAACTT AATCTTTTTG GGATACCATA CATTGGAGCA 
GATATCTGCG GCGTTTTTCA TGATTCAGGA TACCCATCTC TGTACTTCGT TGGGATCCAA 
GTTGGAGCCT TTTATCCATA TCCAAGGGAA TCCCCAACAA TCAATTTTAC TAGAAGCCAA 
GATCCTGTTT CCTGGATGAA ACTTTTGCTC CAAATGTCAA AGAAAGTTCT AGAAATCAGA 
TACACTTTGC TGCCCTATTT CTACACACAG ATGCATGAAG CCCATGCTCA TGGTGGAACT 
GTCATCAGAC CCCTCATGCA TGAGTTCTTT GATGATAAAG AAACCTGGGA AATATACAAG 
CAGTTCCTAT GGGGTCCAGC TTTTATGGTG ACTCCTGTCG TTGAACCTTT CAGGACCAGT 
GTGACTGGCT ATGTTCCTAA GGCTCGATGG TTTGACTACC ATACAGGGGC CGATATAAAG 
CTCAAAGGAA TACTTCACAC ATTTTCAGCT CCCTTTGATA CAATAAATCT ACACGTCCGA 
GGAGGTTACA TTCTACCATG TCAAGAGCCA GCACGGAACA CGCATCTAAG TCGACAGAAT 
TACATGAAGC TCATTGTTGC TGCAGATGAC AACCAAATGG CACAAGGGAC TCTATTTGGG 
GATGATGGAG AGTCTATAGA CACTTATGAA AGAGGTCAAT ACACCTCGAT ACAATTCAAT 
TTAAATCAGA CAACTTTAAC AAGCACTGTA TTGGCAAATG GGTATAAAAA CAAACAGGAG 
ATGAGACTTG GCAGCATCCA CATATGGGGG AAAGGAACAC TCCGTATCAG CAATGCTAAT 
TTGGTTTATG GAGGCCGTAA ACATCAGCCT CCTTTTACTC AAGAGGAAGC TAAGGAGACA 
CTAATATTTG ACCTGAAAAA TATGAATGTT ACTCTTGATG AACCAATACA AATCACCTGG 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for substrate selectivity in human maltase-glucoamylase and sucrase-isomaltase N-terminal domains.
The Journal of biological chemistry285(23)17763-70(2010 Jun)
Sim L,Willemsma C,Mohan S,Naim HY,Pinto BM,Rose DR

Induction of histone acetylation on the sucrase-isomaltase gene in the postnatal rat jejunum.
Bioscience, biotechnology, and biochemistry73(4)933-5(2009 Apr)
Yorita S,Mochizuki K,Goda T

Sodium tungstate decreases sucrase and Na+/D-glucose cotransporter in the jejunum of diabetic rats.
American journal of physiology. Gastrointestinal and liver physiology295(3)G479-84(2008 Sep)
Mir?-Queralt M,Guinovart JJ,Planas JM

Histone H3 modifications and Cdx-2 binding to the sucrase-isomaltase (SI) gene is involved in induction of the gene in the transition from the crypt to villus in the small intestine of rats.
Biochemical and biophysical research communications369(2)788-93(2008 May)
Suzuki T,Mochizuki K,Goda T

Effect of harmaline on rat intestinal brush border sucrase activity.
Indian journal of biochemistry & biophysics39(2)119-23(2002 Apr)
Kaur N,Kaur J,Mahmood A

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Si cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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