Ift81 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ift81
Entrez Gene ID	373066
Full Name	intraflagellar transport 81
Synonyms	Cdv-1,Cdv1
General protein information	Preferred Names intraflagellar transport 81 Names intraflagellar transport protein 81 homolog carnitine deficiency-associated gene expressed in ventricle 1 carnitine deficiency-associated protein expressed in ventricle 1 intraflagellar transport 81 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	mouse homolog is a downregulated gene in the hypertrophied ventricle of carnitine-deficient juvenile visceral steatosis mice [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199120.1	NP_954551.1	intraflagellar transport protein 81 homolog

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002293	XP_307884.3
Pan troglodytes (chimpanzee)	IFT81	XP_001141289.1
Rattus norvegicus (Norway rat)	Ift81	NP_954551.1
Danio rerio (zebrafish)	ift81	NP_001002313.1
Bos taurus (cattle)	IFT81	NP_001074994.1
Mus musculus (house mouse)	Ift81	NP_034009.2
Xenopus tropicalis (tropical clawed frog)	ift81	XP_002937744.2
Homo sapiens (human)	IFT81	NP_001137251.1
	IFT81	XP_001099060.2
Canis lupus familiaris (dog)	IFT81	XP_003639956.1
Gallus gallus (chicken)	IFT81	NP_001239238.1
Caenorhabditis elegans (roundworm)	ift-81	NP_508900.6

Gene Ontology
Bibliography

Related articles in PubMed

Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.
Duran I, Taylor SP, Zhang W, Martin J, Forlenza KN, Spiro RP, Nickerson DA, Bamshad M, Cohn DH, Krakow D
Scientific reports634232(2016 Sep)

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Liew GM, Ye F, Nager AR, Murphy JP, Lee JS, Aguiar M, Breslow DK, Gygi SP, Nachury MV
Developmental cell31(3)265-78(2014 Nov)

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
Ishikawa H, Ide T, Yagi T, Jiang X, Hirono M, Sasaki H, Yanagisawa H, Wemmer KA, Stainier DY, Qin H, Kamiya R, Marshall WF
eLife3e01566(2014 Jan)

Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport complex B proteins and KIF17.
Howard PW, Jue SF, Maurer RA
Experimental cell research319(14)2275-81(2013 Aug)

Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.
Bhogaraju S, Cajanek L, Fort C, Blisnick T, Weber K, Taschner M, Mizuno N, Lamla S, Bastin P, Nigg EA, Lorentzen E
Science (New York, N.Y.)341(6149)1009-12(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ift81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04702	NM_199120.1 Latest version!	Rattus norvegicus intraflagellar transport 81 (Ift81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ift81 ( NM_199120.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954551.1 Rattus norvegicus intraflagellar transport 81 (Ift81), mRNA.

Price & Availability↑

CloneID	ORa04702
Clone ID Related Accession (Same CDS sequence)	NM_199120.1
Accession Version	NM_199120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	intraflagellar transport protein 81 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY345164.1. On Jan 12, 2005 this sequence version replaced XM_222174.2. ##Evidence-Data-START## Transcript exon combination :: AY345164.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGACC AAATCAAATT CATCGTGGAC AGCCTCAATA AGGAGCCTTT TAAGAAGAAC 
TATAATTTAA TCACATTTGA TTCTTTGGGA CCAATGCAAC TATTACAAGT TCTCAATGAT 
GTCCTGGCTG AGATAGACCC CAAGCAAGAC GTTGATATCA GAGAGGAGAC GCCGGAGCAG 
ACAGCCAAGC GCATGTTGAG CCTTCTTGGT ATCCTGAAGT ACAAACCTCC GGGGAATGCC 
ACAGACATGA GCACCTTCCG CCAGGGTTTG GTGATCGGGA GTAAACCTGT CATCTACCCG 
GTGCTCCACT GGCTCCTCCA GCGGAGCAGT GAACTAAAGA AAAGGGCGTA TTTAGCTCGC 
TTCTTAATAA AGCTGGAGGT GCCCAGCGAG TTTCTCCAAG ATGAAACCGT GGCTGACACC 
AACAAACAGT ATGAAGAGCT GATGGAAGCC TTCAAAGCCC TGCATAAAGA GTGTGAGCAG 
CTCAAAACAT CTGGATTCTC CACAGCGGAG ATAAGAAGGG ATATCAGCGC AATGGAGGAG 
GAGAAAGACC AGCTCATGAA GAGAGTTGAA CGGTTGAAGA AGAGGGTGGA GACTGTCCAG 
AACCATCAGC GAATGCTTAA AATAGCAAGA CAGCTTCGGG TTGAGAAAGA GAGAGAAGAG 
TTTCTTGCTC AACAGAAGCA GGAACAAAAG AATCAGCTGT TTCATGCGGT GCAGAGATTG 
CAAAGAGTGC AAAACCAGCT GAAGAGTATG CGGCACGCGG CAGCAGACGC CAAGCCTGAG 
AGTTTAATGA AGAGGCTAGA GGAGGAGATA AAATTTAATT CATATATGGT CACTGAAAAA 
TTTCCAAAAG AATTAGAAAG CAAGAAAAAG GAATTACACT TTTTACAAAA AGTGGTTTCT 
GAGCCAGCTA TGGGCCACTC TGACCTTCTT GAACTTGAGT CTAAAGTCAA TGAAGTAAAC 
GCAGAGATCA ATCAGTTGAT TGAGAAAAAA ATGATGAGAA ACGAGCCAAT AGAAGGCAAG 
CTCTCACTGT ACAGGCAACA GGCGTCCATC ATTTCTCGTA AGAAAGAGGC CAAAGCTGAG 
GAGCTGCAGG AGACCAAGGA GAAGCTGGCC AGCCTCGAGA GAGAGGTGTC TGTGAAGACA 
AACCAGACCC GGGAATTTGA CGGCACTGAG GTTCTGAAGG GAGATGAGTT CAAGAGATAC 
GTCAGTAAAC TTCGGAGCAA AAGCACAGTT TTCAAAAAGA AGCACCAGAT AATAGCTGAG 
TTTAAGGCTG AATTTGGCCT CTTGCAGAGG ACAGAAGAAC TTCTGAAGCA ACGTCAGGAA 
ACCATTCAAC ATCAACTGCG AACCATTGAA GAGAAGAAGG GCATCTCTGG TTACAGCTAC 
ACCCAAGAGG AGCTTGAGAG AGTGTCGGCC CTGAAGAGCG AGGTTGACGA GATGAAAGGC 
CGGACCCTGG ACGACATGTC AGAGATGGTA AAAAAACTGA ATTCCTTGGT ATCAGAAAAG 
AAGTCAGCTT TAGCCCCAGT TATAAAAGAA CTACGGCAAT TGCGTCAGAA ATGTCAGCTA 
ACCCAGGAGT GTGATGAGAA GAGAACCCAG TATGATAGCT GTGCGGCAGG CCTTGAAAGC 
AACCGGTCCA AATTAGAGCA GGAAGTACGA GGACTCCGTG AAGAATGTCT TCAAGAAGAA 
AGTAAATACC ATTATACAAA CTGCATGATT AAGAACCTAG AAGTTCAGCT TCGCCGAGCC 
ACTGATGAGA TGAAGGCGTA CGTCTCTTCT GATCAACAAG AAAAGAGGAA AGCAATTAGG 
GAACAGTATA CCAAAAATAT TACTGAGCAA GAAAACCTGG GCAAGAAACT TCGGGAAAAA 
CAAAAGGCTG TTCGAGAAAG TCACGGTCCC AACATGAAGC AAGCGAAGAT GTGGCGGGAT 
CTTGAACAAC TGATGGAATG CAAAAAACAG TGCTTTCTGA AGCAGCAGAG CCCAGCCTCC 
ATCGGCCAGG TAATTCAGGA GGGCGGAGAG GACCGGCTGG TTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954551.1
CDS	103..2130
Translation	MSDQIKFIVDSLNKEPFKKNYNLITFDSLGPMQLLQVLNDVLAEIDPKQDVDIREETPEQTAKRMLSLLGILKYKPPGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRSSELKKRAYLARFLIKLEVPSEFLQDETVADTNKQYEELMEAFKALHKECEQLKTSGFSTAEIRRDISAMEEEKDQLMKRVERLKKRVETVQNHQRMLKIARQLRVEKEREEFLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRHAAADAKPESLMKRLEEEIKFNSYMVTEKFPKELESKKKELHFLQKVVSEPAMGHSDLLELESKVNEVNAEINQLIEKKMMRNEPIEGKLSLYRQQASIISRKKEAKAEELQETKEKLASLEREVSVKTNQTREFDGTEVLKGDEFKRYVSKLRSKSTVFKKKHQIIAEFKAEFGLLQRTEELLKQRQETIQHQLRTIEEKKGISGYSYTQEELERVSALKSEVDEMKGRTLDDMSEMVKKLNSLVSEKKSALAPVIKELRQLRQKCQLTQECDEKRTQYDSCAAGLESNRSKLEQEVRGLREECLQEESKYHYTNCMIKNLEVQLRRATDEMKAYVSSDQQEKRKAIREQYTKNITEQENLGKKLREKQKAVRESHGPNMKQAKMWRDLEQLMECKKQCFLKQQSPASIGQVIQEGGEDRLVL

Target ORF information:

RefSeq Version	NM_199120.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus intraflagellar transport 81 (Ift81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199120.1

ORF Insert Sequence:

ATGAGTGACC AAATCAAATT CATCGTGGAC AGCCTCAATA AGGAGCCTTT TAAGAAGAAC 
TATAATTTAA TCACATTTGA TTCTTTGGGA CCAATGCAAC TATTACAAGT TCTCAATGAT 
GTCCTGGCTG AGATAGACCC CAAGCAAGAC GTTGATATCA GAGAGGAGAC GCCGGAGCAG 
ACAGCCAAGC GCATGTTGAG CCTTCTTGGT ATCCTGAAGT ACAAACCTCC GGGGAATGCC 
ACAGACATGA GCACCTTCCG CCAGGGTTTG GTGATCGGGA GTAAACCTGT CATCTACCCG 
GTGCTCCACT GGCTCCTCCA GCGGAGCAGT GAACTAAAGA AAAGGGCGTA TTTAGCTCGC 
TTCTTAATAA AGCTGGAGGT GCCCAGCGAG TTTCTCCAAG ATGAAACCGT GGCTGACACC 
AACAAACAGT ATGAAGAGCT GATGGAAGCC TTCAAAGCCC TGCATAAAGA GTGTGAGCAG 
CTCAAAACAT CTGGATTCTC CACAGCGGAG ATAAGAAGGG ATATCAGCGC AATGGAGGAG 
GAGAAAGACC AGCTCATGAA GAGAGTTGAA CGGTTGAAGA AGAGGGTGGA GACTGTCCAG 
AACCATCAGC GAATGCTTAA AATAGCAAGA CAGCTTCGGG TTGAGAAAGA GAGAGAAGAG 
TTTCTTGCTC AACAGAAGCA GGAACAAAAG AATCAGCTGT TTCATGCGGT GCAGAGATTG 
CAAAGAGTGC AAAACCAGCT GAAGAGTATG CGGCACGCGG CAGCAGACGC CAAGCCTGAG 
AGTTTAATGA AGAGGCTAGA GGAGGAGATA AAATTTAATT CATATATGGT CACTGAAAAA 
TTTCCAAAAG AATTAGAAAG CAAGAAAAAG GAATTACACT TTTTACAAAA AGTGGTTTCT 
GAGCCAGCTA TGGGCCACTC TGACCTTCTT GAACTTGAGT CTAAAGTCAA TGAAGTAAAC 
GCAGAGATCA ATCAGTTGAT TGAGAAAAAA ATGATGAGAA ACGAGCCAAT AGAAGGCAAG 
CTCTCACTGT ACAGGCAACA GGCGTCCATC ATTTCTCGTA AGAAAGAGGC CAAAGCTGAG 
GAGCTGCAGG AGACCAAGGA GAAGCTGGCC AGCCTCGAGA GAGAGGTGTC TGTGAAGACA 
AACCAGACCC GGGAATTTGA CGGCACTGAG GTTCTGAAGG GAGATGAGTT CAAGAGATAC 
GTCAGTAAAC TTCGGAGCAA AAGCACAGTT TTCAAAAAGA AGCACCAGAT AATAGCTGAG 
TTTAAGGCTG AATTTGGCCT CTTGCAGAGG ACAGAAGAAC TTCTGAAGCA ACGTCAGGAA 
ACCATTCAAC ATCAACTGCG AACCATTGAA GAGAAGAAGG GCATCTCTGG TTACAGCTAC 
ACCCAAGAGG AGCTTGAGAG AGTGTCGGCC CTGAAGAGCG AGGTTGACGA GATGAAAGGC 
CGGACCCTGG ACGACATGTC AGAGATGGTA AAAAAACTGA ATTCCTTGGT ATCAGAAAAG 
AAGTCAGCTT TAGCCCCAGT TATAAAAGAA CTACGGCAAT TGCGTCAGAA ATGTCAGCTA 
ACCCAGGAGT GTGATGAGAA GAGAACCCAG TATGATAGCT GTGCGGCAGG CCTTGAAAGC 
AACCGGTCCA AATTAGAGCA GGAAGTACGA GGACTCCGTG AAGAATGTCT TCAAGAAGAA 
AGTAAATACC ATTATACAAA CTGCATGATT AAGAACCTAG AAGTTCAGCT TCGCCGAGCC 
ACTGATGAGA TGAAGGCGTA CGTCTCTTCT GATCAACAAG AAAAGAGGAA AGCAATTAGG 
GAACAGTATA CCAAAAATAT TACTGAGCAA GAAAACCTGG GCAAGAAACT TCGGGAAAAA 
CAAAAGGCTG TTCGAGAAAG TCACGGTCCC AACATGAAGC AAGCGAAGAT GTGGCGGGAT 
CTTGAACAAC TGATGGAATG CAAAAAACAG TGCTTTCTGA AGCAGCAGAG CCCAGCCTCC 
ATCGGCCAGG TAATTCAGGA GGGCGGAGAG GACCGGCTGG TTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.
Scientific reports634232(2016 Sep)
Duran I,Taylor SP,Zhang W,Martin J,Forlenza KN,Spiro RP,Nickerson DA,Bamshad M,Cohn DH,Krakow D

The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3.
Developmental cell31(3)265-78(2014 Nov)
Liew GM,Ye F,Nager AR,Murphy JP,Lee JS,Aguiar M,Breslow DK,Gygi SP,Nachury MV

TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella.
eLife3e01566(2014 Jan)
Ishikawa H,Ide T,Yagi T,Jiang X,Hirono M,Sasaki H,Yanagisawa H,Wemmer KA,Stainier DY,Qin H,Kamiya R,Marshall WF

Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport complex B proteins and KIF17.
Experimental cell research319(14)2275-81(2013 Aug)
Howard PW,Jue SF,Maurer RA

Molecular basis of tubulin transport within the cilium by IFT74 and IFT81.
Science (New York, N.Y.)341(6149)1009-12(2013 Aug)
Bhogaraju S,Cajanek L,Fort C,Blisnick T,Weber K,Taschner M,Mizuno N,Lamla S,Bastin P,Nigg EA,Lorentzen E

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Ift81 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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