Pif1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pif1
Entrez Gene ID	367645
Full Name	PIF1 5'-to-3' DNA helicase
General protein information	Preferred Names PIF1 5'-to-3' DNA helicase Names ATP-dependent DNA helicase PIF1 DNA repair and recombination helicase PIF1 PIF1/RRM3 DNA helicase-like protein Pif1/Rrm3 DNA-helicase-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766324.1	XP_008764546.1	ATP-dependent DNA helicase PIF1 isoform X1
XM_008766326.1	XP_008764548.1	ATP-dependent DNA helicase PIF1 isoform X1
XM_006243271.2	XP_006243333.1	ATP-dependent DNA helicase PIF1 isoform X1
XM_008766325.1	XP_008764547.1	ATP-dependent DNA helicase PIF1 isoform X1
XM_008766327.1	XP_008764549.1	ATP-dependent DNA helicase PIF1 isoform X1
NM_001044253.1	NP_001037718.1	ATP-dependent DNA helicase PIF1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pif1	NP_766041.1
Caenorhabditis elegans (roundworm)	pif-1	NP_490774.2
Xenopus tropicalis (tropical clawed frog)	pif1	NP_001016603.1
Homo sapiens (human)	PIF1	NP_001273426.1
	PIF1	XP_001108233.1
Gallus gallus (chicken)	PIF1	XP_426648.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007775	XP_317740.4
Xenopus tropicalis (tropical clawed frog)	LOC101730335	XP_004914011.1
Bos taurus (cattle)	PIF1	NP_001192981.1
Rattus norvegicus (Norway rat)	Pif1	NP_001037718.1
Danio rerio (zebrafish)	pif1	NP_942102.1
Pan troglodytes (chimpanzee)	PIF1	XP_510475.2
Canis lupus familiaris (dog)	PIF1	XP_003640106.1
Drosophila melanogaster (fruit fly)	CG3238	NP_608782.1

Gene Ontology
Bibliography

Related articles in PubMed

Pif1 family helicases suppress genome instability at G-quadruplex motifs.
Paeschke K, Bochman ML, Garcia PD, Cejka P, Friedman KL, Kowalczykowski SC, Zakian VA
Nature497(7450)458-62(2013 May)

Alternative translation initiation augments the human mitochondrial proteome.
Kazak L, Reyes A, Duncan AL, Rorbach J, Wood SR, Brea-Calvo G, Gammage PA, Robinson AJ, Minczuk M, Holt IJ
Nucleic acids research41(4)2354-69(2013 Feb)

The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity.
Zhang DH, Zhou B, Huang Y, Xu LX, Zhou JQ
Nucleic acids research34(5)1393-404(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12543	NM_001044253.1 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa31303	XM_006243271.2 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa31303	XM_008766325.1 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa31303	XM_008766327.1 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa31303	XM_008766324.1 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa31303	XM_008766326.1 Latest version!	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pif1 ( NM_001044253.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001037718.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), mRNA.

Price & Availability↑

CloneID	ORa12543
Clone ID Related Accession (Same CDS sequence)	NM_001044253.1
Accession Version	NM_001044253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ489562.1. On or before Aug 24, 2006 this sequence version replaced XM_346237.3, XM_001076669.1. ##Evidence-Data-START## Transcript exon combination :: DQ489562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGAGTCG 
CAAGACGATG AGGAGGCAAG CTCAGATCTG GAGAACATGG ACCCAAACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037718.1
CDS	2..1915
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLESQDDEEASSDLENMDPNL

Target ORF information:

RefSeq Version	NM_001044253.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044253.1

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGAGTCG 
CAAGACGATG AGGAGGCAAG CTCAGATCTG GAGAACATGG ACCCAAACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( XM_008766324.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764546.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31303
Clone ID Related Accession (Same CDS sequence)	XM_008766324.1 , XM_008766326.1 , XM_006243271.2 , XM_008766325.1 , XM_008766327.1
Accession Version	XM_008766324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764546.1
CDS	658..2664
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLVRSHLKRALLGVGRLRRRPSSESLLIPFWWRQKERGVGPQAVALGIGTNL

Target ORF information:

RefSeq Version	XM_008766324.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766324.1

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( XM_008766326.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764548.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa31303
Clone ID Related Accession (Same CDS sequence)	XM_008766324.1 , XM_008766326.1 , XM_006243271.2 , XM_008766325.1 , XM_008766327.1
Accession Version	XM_008766326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764548.1
CDS	589..2595
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLVRSHLKRALLGVGRLRRRPSSESLLIPFWWRQKERGVGPQAVALGIGTNL

Target ORF information:

RefSeq Version	XM_008766326.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766326.1

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( XM_006243271.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243333.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31303
Clone ID Related Accession (Same CDS sequence)	XM_008766324.1 , XM_008766326.1 , XM_006243271.2 , XM_008766325.1 , XM_008766327.1
Accession Version	XM_006243271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006243271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243333.1
CDS	939..2945
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLVRSHLKRALLGVGRLRRRPSSESLLIPFWWRQKERGVGPQAVALGIGTNL

Target ORF information:

RefSeq Version	XM_006243271.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243271.2

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( XM_008766325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764547.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa31303
Clone ID Related Accession (Same CDS sequence)	XM_008766324.1 , XM_008766326.1 , XM_006243271.2 , XM_008766325.1 , XM_008766327.1
Accession Version	XM_008766325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764547.1
CDS	630..2636
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLVRSHLKRALLGVGRLRRRPSSESLLIPFWWRQKERGVGPQAVALGIGTNL

Target ORF information:

RefSeq Version	XM_008766325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766325.1

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1 ( XM_008766327.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764549.1 Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X5, mRNA.

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CloneID	ORa31303
Clone ID Related Accession (Same CDS sequence)	XM_008766324.1 , XM_008766326.1 , XM_006243271.2 , XM_008766325.1 , XM_008766327.1
Accession Version	XM_008766327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA helicase PIF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764549.1
CDS	561..2567
Translation	MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFPLRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLLGPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAVLRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQCVALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGSQHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATRLCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLVNGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDCVEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLVRSHLKRALLGVGRLRRRPSSESLLIPFWWRQKERGVGPQAVALGIGTNL

Target ORF information:

RefSeq Version	XM_008766327.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PIF1 5'-to-3' DNA helicase (Pif1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766327.1

ORF Insert Sequence:

ATGCCTTCTA GCACTGAGGT GGCGACCGAT GAATGTGATG ACACCGAGCT GCGGTGCCGG 
GTAGCCGTAG AGGAGCTGAG TCCTGGAGGG CAACCTCGCA AGCGCCAGGC TCTGCGCGCC 
GCAGAGCTGA GCCTAGGTCG GAATGAACGC CGCGAGTTGA TGCTGCGACT GCAGGCACCC 
GGACCCGAAG GGCGGCCACG CTGTTTCCCA CTGCGCGCCG TGCGCCTCTT CACCCGCTTC 
GCCGCAGTTG GGCGCAGCAC TTTGCGACTC CCGGCCGATG GCGTGCCCCG GGCTGGCTCA 
GTGCAACTGC TGCTCTCTGA CTGTCCCCCG GAGCGCTTGC GCCGCTTCCT GCGCACCCTG 
CGCCTGAAGC TGGCGGTCGC CCCTGGGCCG GGACCCGCCT CTGCCCGTGC GCAATTGCTC 
GGTCCGCGGC CCCGAGACTT TGTCACGATC AGTCCAGTGC AGCCGGAGGA ACTGCGCCGT 
GCTGCGGCCA CCAAGGCTCC AGACTCAGCG CTGGAAAAGC GGCCAATGGA ATCCCAGCCT 
AGTATGGAAG CTCCAAGGTG GCCCCTGCCT GTGAAGAAGC TACGCATGCC CTCCAGCAAA 
CCGAAGCTTT CCGAAGAGCA GGCCGCTGTG CTGCGGATGG TTCTGAAAGG CCAGAGCATA 
TTCTTTACCG GGAGTGCAGG GACAGGGAAG TCCTACCTGC TGAAACATAT CCTGGGTTCC 
CTGCCTCCTA CTGGCACTGT GGCCACTGCC AGCACTGGGG TGGCAGCCTG CCACATTGGG 
GGCACCACCC TTCATGCCTT TGCAGGCATC GGTTCAGGCC AGGCTCCCCT GGCCCAGTGT 
GTGGCTCTGG CCCATCGGCC TGGTGTGCGG CAGGGCTGGC TGAACTGCCA GCGTTTGGTC 
ATTGATGAGA TCTCCATGGT AGAGGCAGAC TTCTTTGACA AGTTGGAAGC TGTGGCCAGA 
GCTGTCCGGC AACAGAAGAA ACCATTTGGA GGGATCCAGC TTATTATCTG TGGGGACTTC 
CTACAGTTGC CGCCAGTGAC CAAAGGTTCC CAGCATCCTC GATTCTGCTT TCAGGCCAAG 
AGCTGGAGGA AGTGTGTACC AGTGACTCTG GAGCTGACTG AGGTGTGGAG GCAAGCAGAC 
CAGACCTTCA TCTCTCTCCT GAAGGCTGTG AGATTAGGCA GATGTTCAGA TGAAGTGACC 
CGCCAGCTCA GGGCCACAGC TGCCCATAAG GTGGGACGAG ATGGAATTAT AGCCACGAGA 
CTGTGCACCC ATCAGGATGA TGTGGCCCTT ACCAATGAGA AGCGGCTGAA GGAACTACCA 
GGTGATGTAC ACAGCTTTGA GGCTATAGAC AGTGACCCTG AGCTAAGCCG GACCCTGGAT 
GCTCAGTGCC CTGTAGGCCG TGTCCTTCAG TTAAAGCTGG GGGCTCAAGT CATGCTGGTG 
AAGAACTTAG CAGTGTCTCG GGGCCTGGTG AATGGTGCCC GAGGGGTGGT AGTCGGGTTT 
GAGTCAGAAG GAAGAGGGCT TCCCCGGGTA CGGTTCCTGT GTGGCATCAC TGAGGTCATC 
CGCACCGACC GCTGGACAGT ACAGGTCACT GGGGGCCAGT ACCTCAGCCG GCAGCAGCTT 
CCCCTACAGC TAGCCTGGGC AATGTCCATC CACAAAAGCC AGGGCATGTC TCTGGACTGT 
GTAGAGATCT CTCTGGGCCG TGTGTTCGCC AGTGGTCAAG CCTATGTGGC CCTCTCCCGG 
GCCCGTAGCC TCCAGGGTCT ACGTGTGCTA GACTTCGACC CCACTGTGGT TCGATGCGAC 
TCCCGAGTGC TGCAATTCTA TGCCACCCTG CGGCAGGGCA GGGGCCTCAG TCTGGTAAGA 
AGTCACCTAA AGAGGGCCCT CCTGGGTGTG GGCAGGCTGA GAAGGAGGCC TAGCAGTGAA 
TCTTTACTGA TTCCTTTCTG GTGGAGGCAG AAGGAAAGAG GTGTTGGTCC CCAGGCTGTG 
GCCCTGGGGA TTGGCACAAA CCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pif1 family helicases suppress genome instability at G-quadruplex motifs.
Nature497(7450)458-62(2013 May)
Paeschke K,Bochman ML,Garcia PD,Cejka P,Friedman KL,Kowalczykowski SC,Zakian VA

Alternative translation initiation augments the human mitochondrial proteome.
Nucleic acids research41(4)2354-69(2013 Feb)
Kazak L,Reyes A,Duncan AL,Rorbach J,Wood SR,Brea-Calvo G,Gammage PA,Robinson AJ,Minczuk M,Holt IJ

The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity.
Nucleic acids research34(5)1393-404(2006)
Zhang DH,Zhou B,Huang Y,Xu LX,Zhou JQ

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Pif1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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