Zdhhc17 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zdhhc17
Entrez Gene ID	366889
Full Name	zinc finger, DHHC-type containing 17
General protein information	Preferred Names zinc finger, DHHC-type containing 17 Names palmitoyltransferase ZDHHC17 membrane-associated DHHC17 zinc finger protein zinc finger DHHC domain-containing protein 17 zinc finger, DHHC domain containing 17
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595017.1	XP_017450506.1	palmitoyltransferase ZDHHC17 isoform X1
NM_001039340.1	NP_001034429.1	palmitoyltransferase ZDHHC17

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ZDHHC17	NP_001192998.1
Arabidopsis thaliana (thale cress)	TIP1	NP_197535.2
Homo sapiens (human)	ZDHHC17	NP_056151.2
Canis lupus familiaris (dog)	ZDHHC17	XP_005629482.1
Saccharomyces cerevisiae (baker's yeast)	AKR1	NP_010550.1
Oryza sativa (rice)	Os06g0644500	NP_001058187.1
Pan troglodytes (chimpanzee)	ZDHHC17	XP_509231.3
Mus musculus (house mouse)	Zdhhc17	NP_766142.2
Caenorhabditis elegans (roundworm)	tag-233	NP_001024514.2
Magnaporthe oryzae (rice blast fungus)	MGG_07382	XP_003711258.1
Xenopus tropicalis (tropical clawed frog)	zdhhc17	XP_002935687.2
	ZDHHC17	XP_001082309.2
Gallus gallus (chicken)	ZDHHC17	NP_001025916.1
Danio rerio (zebrafish)	zdhhc17	NP_001121854.1
Drosophila melanogaster (fruit fly)	Hip14	NP_648824.1
Oryza sativa (rice)	Os02g0184000	NP_001046105.1
Rattus norvegicus (Norway rat)	Zdhhc17	NP_001034429.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011732	XP_320777.3
Kluyveromyces lactis	KLLA0E16721g	XP_454703.1
Eremothecium gossypii	AGOS_AER388C	NP_985243.1
Schizosaccharomyces pombe (fission yeast)	akr1	NP_592981.1
Neurospora crassa	NCU08218	XP_959308.2

Gene Ontology
Bibliography

Related articles in PubMed

The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.
Lemonidis K, Gorleku OA, Sanchez-Perez MC, Grefen C, Chamberlain LH
Molecular biology of the cell25(24)3870-83(2014 Dec)

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.
Butland SL, Sanders SS, Schmidt ME, Riechers SP, Lin DT, Martin DD, Vaid K, Graham RK, Singaraja RR, Wanker EE, Conibear E, Hayden MR
Human molecular genetics23(15)4142-60(2014 Aug)

Analysis of substrate specificity of human DHHC protein acyltransferases using a yeast expression system.
Ohno Y, Kashio A, Ogata R, Ishitomi A, Yamazaki Y, Kihara A
Molecular biology of the cell23(23)4543-51(2012 Dec)

Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein regulating insulin secretion and beta-cell apoptosis.
Berchtold LA, St?rling ZM, Ortis F, Lage K, Bang-Berthelsen C, Bergholdt R, Hald J, Brorsson CA, Eizirik DL, Pociot F, Brunak S, St?rling J
Proceedings of the National Academy of Sciences of the United States of America108(37)E681-8(2011 Sep)

Huntingtin-interacting proteins, HIP14 and HIP14L, mediate dual functions, palmitoyl acyltransferase and Mg2+ transport.
Goytain A, Hines RM, Quamme GA
The Journal of biological chemistry283(48)33365-74(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunohistochemical analysis of pancreatic sections demonstrated that HIP14 is almost exclusively expressed in insulin-positive cells in islets of Langerhans.
Title: Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein regulating insulin secretion and beta-cell apoptosis.

The following Zdhhc17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zdhhc17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09725	NM_001039340.1 Latest version!	Rattus norvegicus zinc finger DHHC-type palmitoyltransferase 17 (Zdhhc17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
ORa62113	XM_017595017.1 Latest version!	Rattus norvegicus zinc finger, DHHC-type containing 17 (Zdhhc17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zdhhc17 ( NM_001039340.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034429.1 Rattus norvegicus zinc finger DHHC-type palmitoyltransferase 17 (Zdhhc17), mRNA.

Price & Availability↑

CloneID	ORa09725
Clone ID Related Accession (Same CDS sequence)	NM_001039340.1
Accession Version	NM_001039340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Rattus norvegicus(Norway rat)
Product	palmitoyltransferase ZDHHC17
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY886533.1. ##Evidence-Data-START## Transcript exon combination :: AY886533.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACG GCCCGGATGA GTACGATACC GAAACGGGCT GTGTGCCCCT TCTCCACCCG 
GAGGAGATCA AACCACAGAG CCATTATAAC CATGGGTATG GTGAGCCTCT TGGGCGGAAA 
ACCCATGTTG ATGACTACAG CACATGGGAC ATTGTCAAGG CTACACAATA CGGAATATAT 
GAACGCTGCC GAGAATTGGT GGAAGCAGGT TATGACGTAC GGCAACCGGA CAAAGAGAAC 
GTTACACTTC TTCACTGGGC TGCCATCAAT AACAGAATAG ATTTAGTCAA ATACTATATT 
TCGAAAGGTG CTATTGTGGA TCAACTTGGA GGAGACCTGA ATTCAACTCC ATTGCACTGG 
GCCACAAGAC AAGGCCATTT ATCCATGGTT GTACAACTAA TGAAATATGG TGCAGATCCC 
TCATTGATCG ATGGTGAAGG GTGCAGCTGT ATCCACTTGG CTGCTCAGTT TGGACATACC 
TCAATTGTTG CTTACCTTAT AGCAAAAGGA CAGGATGTAG ATATGATGGA TCAGAACGGA 
ATGACACCTT TAATGTGGGC AGCTTATAGA ACACATAGTG TGGATCCAAC TAGATTGCTT 
TTAACATTCA ATGTTTCAGT TAACCTTGGT GACAAGTATC ACAAAAACAC TGCTCTGCAC 
TGGGCAGTCC TAGCAGGGAA CACCACAGTC ATTAGTCTTC TTCTGGAAGC TGGAGGGAAC 
GTGGATGCCC AGAATGTCAA GGGCGAATCA GCACTTGATT TGGCAAAGCA AAGAAAAAAC 
GTGTGGATGA TCAACCATTT GCAGGAGGCA AGGCAGGCGA AGGGTTACGA CAACCCGTCC 
TTCCTCAGAA AGCTGAAGGC TGACAAGGAA TTTCGGCAGA AAGTAATGCT AGGAACTCCA 
TTCCTAGTGA TTTGGCTAGT TGGGTTTATA GCAGACCTGA ACATTGATTC TTGGCTCATT 
AAAGGGTTAA TGTATGGTGG TGTTTGGGCT ACAGTACAAT TTCTTTCCAA GTCCTTTTTT 
GATCATTCAA TGCACAGCGC ATTGCCCCTT GGGATATATT TGGCAACCAA ATTCTGGATG 
TATGTGACAT GGTTCTTCTG GTTTTGGAAT GATCTCAGCT TCTTATCCAT CCACCTGCCC 
TTCCTCGCCA ACAGTGTTGC TCTTTTCTAC AATTTTGGAA AGTCTTGGAA ATCAGATCCA 
GGGATCATTA AAGCTACAGA AGAACAAAAG AAGAAGACAA TAGTTGAACT TGCAGAGACC 
GGCAGTCTGG ACCTCAGTAT ATTCTGCAGC ACCTGCTTGA TTCGGAAGCC GGTGAGGTCC 
AAACACTGTG GTGTGTGCAA CCGCTGTATA GCGAAATTTG ATCACCATTG CCCATGGGTG 
GGTAACTGTG TATGTGCAGG CAACCATAGA TACTTTATGG GCTACCTATT CTTCTTGCTT 
TTTATGATCT GCTGGATGAT TTATGGTTGT GTTTCTTACT GGGGACTTCA CTGTGAGACC 
ACGTACACGA AGGATGGCTT CTGGACATAT ATTACTCAGA TTGCCACATG CTCTCCTTGG 
ATGTTTTGGA TGTTTCTGAA CAGTGTGTTT CATTTCATGT GGGTGGCCGT GCTACTCATG 
TGTCAGATGT ACCAGATAAC ATGTTTAGGT ATTACTACAA ATGAAAGAAT GAATGCCAGG 
AGATACAAGC ACTTTAAAGT TACAACAACA TCTATAGAGA GCCCATTCAA CCATGGATGT 
GTAAGGAATA TTATAGACTT CTTTGAATTT CGATGCTGTG GCCTCTTTCG TCCTGTTATC 
GTGGACTGGA CCAGGCAATA CACAATAGAG TATGACCAAA TATCAGGATC TGGGTACCAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034429.1
CDS	9..1877
Translation	MADGPDEYDTETGCVPLLHPEEIKPQSHYNHGYGEPLGRKTHVDDYSTWDIVKATQYGIYERCRELVEAGYDVRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMVVQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHSVDPTRLLLTFNVSVNLGDKYHKNTALHWAVLAGNTTVISLLLEAGGNVDAQNVKGESALDLAKQRKNVWMINHLQEARQAKGYDNPSFLRKLKADKEFRQKVMLGTPFLVIWLVGFIADLNIDSWLIKGLMYGGVWATVQFLSKSFFDHSMHSALPLGIYLATKFWMYVTWFFWFWNDLSFLSIHLPFLANSVALFYNFGKSWKSDPGIIKATEEQKKKTIVELAETGSLDLSIFCSTCLIRKPVRSKHCGVCNRCIAKFDHHCPWVGNCVCAGNHRYFMGYLFFLLFMICWMIYGCVSYWGLHCETTYTKDGFWTYITQIATCSPWMFWMFLNSVFHFMWVAVLLMCQMYQITCLGITTNERMNARRYKHFKVTTTSIESPFNHGCVRNIIDFFEFRCCGLFRPVIVDWTRQYTIEYDQISGSGYQLV

Target ORF information:

RefSeq Version	NM_001039340.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger DHHC-type palmitoyltransferase 17 (Zdhhc17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039340.1

ORF Insert Sequence:

ATGGCGGACG GCCCGGATGA GTACGATACC GAAACGGGCT GTGTGCCCCT TCTCCACCCG 
GAGGAGATCA AACCACAGAG CCATTATAAC CATGGGTATG GTGAGCCTCT TGGGCGGAAA 
ACCCATGTTG ATGACTACAG CACATGGGAC ATTGTCAAGG CTACACAATA CGGAATATAT 
GAACGCTGCC GAGAATTGGT GGAAGCAGGT TATGACGTAC GGCAACCGGA CAAAGAGAAC 
GTTACACTTC TTCACTGGGC TGCCATCAAT AACAGAATAG ATTTAGTCAA ATACTATATT 
TCGAAAGGTG CTATTGTGGA TCAACTTGGA GGAGACCTGA ATTCAACTCC ATTGCACTGG 
GCCACAAGAC AAGGCCATTT ATCCATGGTT GTACAACTAA TGAAATATGG TGCAGATCCC 
TCATTGATCG ATGGTGAAGG GTGCAGCTGT ATCCACTTGG CTGCTCAGTT TGGACATACC 
TCAATTGTTG CTTACCTTAT AGCAAAAGGA CAGGATGTAG ATATGATGGA TCAGAACGGA 
ATGACACCTT TAATGTGGGC AGCTTATAGA ACACATAGTG TGGATCCAAC TAGATTGCTT 
TTAACATTCA ATGTTTCAGT TAACCTTGGT GACAAGTATC ACAAAAACAC TGCTCTGCAC 
TGGGCAGTCC TAGCAGGGAA CACCACAGTC ATTAGTCTTC TTCTGGAAGC TGGAGGGAAC 
GTGGATGCCC AGAATGTCAA GGGCGAATCA GCACTTGATT TGGCAAAGCA AAGAAAAAAC 
GTGTGGATGA TCAACCATTT GCAGGAGGCA AGGCAGGCGA AGGGTTACGA CAACCCGTCC 
TTCCTCAGAA AGCTGAAGGC TGACAAGGAA TTTCGGCAGA AAGTAATGCT AGGAACTCCA 
TTCCTAGTGA TTTGGCTAGT TGGGTTTATA GCAGACCTGA ACATTGATTC TTGGCTCATT 
AAAGGGTTAA TGTATGGTGG TGTTTGGGCT ACAGTACAAT TTCTTTCCAA GTCCTTTTTT 
GATCATTCAA TGCACAGCGC ATTGCCCCTT GGGATATATT TGGCAACCAA ATTCTGGATG 
TATGTGACAT GGTTCTTCTG GTTTTGGAAT GATCTCAGCT TCTTATCCAT CCACCTGCCC 
TTCCTCGCCA ACAGTGTTGC TCTTTTCTAC AATTTTGGAA AGTCTTGGAA ATCAGATCCA 
GGGATCATTA AAGCTACAGA AGAACAAAAG AAGAAGACAA TAGTTGAACT TGCAGAGACC 
GGCAGTCTGG ACCTCAGTAT ATTCTGCAGC ACCTGCTTGA TTCGGAAGCC GGTGAGGTCC 
AAACACTGTG GTGTGTGCAA CCGCTGTATA GCGAAATTTG ATCACCATTG CCCATGGGTG 
GGTAACTGTG TATGTGCAGG CAACCATAGA TACTTTATGG GCTACCTATT CTTCTTGCTT 
TTTATGATCT GCTGGATGAT TTATGGTTGT GTTTCTTACT GGGGACTTCA CTGTGAGACC 
ACGTACACGA AGGATGGCTT CTGGACATAT ATTACTCAGA TTGCCACATG CTCTCCTTGG 
ATGTTTTGGA TGTTTCTGAA CAGTGTGTTT CATTTCATGT GGGTGGCCGT GCTACTCATG 
TGTCAGATGT ACCAGATAAC ATGTTTAGGT ATTACTACAA ATGAAAGAAT GAATGCCAGG 
AGATACAAGC ACTTTAAAGT TACAACAACA TCTATAGAGA GCCCATTCAA CCATGGATGT 
GTAAGGAATA TTATAGACTT CTTTGAATTT CGATGCTGTG GCCTCTTTCG TCCTGTTATC 
GTGGACTGGA CCAGGCAATA CACAATAGAG TATGACCAAA TATCAGGATC TGGGTACCAG 
CTGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zdhhc17 ( XM_017595017.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450506.1 Rattus norvegicus zinc finger, DHHC-type containing 17 (Zdhhc17), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62113
Clone ID Related Accession (Same CDS sequence)	XM_017595017.1
Accession Version	XM_017595017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	palmitoyltransferase ZDHHC17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGG AGGAGGGATT TAACACCAAG ATGGCGGACG GCCCGGATGA GTACGATACC 
GAAACGGGCT GTGTGCCCCT TCTCCACCCG GAGGAGATCA AACCACAGAG CCATTATAAC 
CATGGGTATG GTGAGCCTCT TGGGCGGAAA ACCCATGTTG ATGACTACAG CACATGGGAC 
ATTGTCAAGG CTACACAATA CGGAATATAT GAACGCTGCC GAGAATTGGT GGAAGCAGGT 
TATGACGTAC GGCAACCGGA CAAAGAGAAC GTTACACTTC TTCACTGGGC TGCCATCAAT 
AACAGAATAG ATTTAGTCAA ATACTATATT TCGAAAGGTG CTATTGTGGA TCAACTTGGA 
GGAGACCTGA ATTCAACTCC ATTGCACTGG GCCACAAGAC AAGGCCATTT ATCCATGGTT 
GTACAACTAA TGAAATATGG TGCAGATCCC TCATTGATCG ATGGTGAAGG GTGCAGCTGT 
ATCCACTTGG CTGCTCAGTT TGGACATACC TCAATTGTTG CTTACCTTAT AGCAAAAGGA 
CAGGATGTAG ATATGATGGA TCAGAACGGA ATGACACCTT TAATGTGGGC AGCTTATAGA 
ACACATAGTG TGGATCCAAC TAGATTGCTT TTAACATTCA ATGTTTCAGT TAACCTTGGT 
GACAAGTATC ACAAAAACAC TGCTCTGCAC TGGGCAGTCC TAGCAGGGAA CACCACAGTC 
ATTAGTCTTC TTCTGGAAGC TGGAGGGAAC GTGGATGCCC AGAATGTCAA GGGCGAATCA 
GCACTTGATT TGGCAAAGCA AAGAAAAAAC GTGTGGATGA TCAACCATTT GCAGGAGGCA 
AGGCAGGCGA AGGGTTACGA CAACCCGTCC TTCCTCAGAA AGCTGAAGGC TGACAAGGAA 
TTTCGGCAGA AAGTAATGCT AGGAACTCCA TTCCTAGTGA TTTGGCTAGT TGGGTTTATA 
GCAGACCTGA ACATTGATTC TTGGCTCATT AAAGGGTTAA TGTATGGTGG TGTTTGGGCT 
ACAGTACAAT TTCTTTCCAA GTCCTTTTTT GATCATTCAA TGCACAGCGC ATTGCCCCTT 
GGGATATATT TGGCAACCAA ATTCTGGATG TATGTGACAT GGTTCTTCTG GTTTTGGAAT 
GATCTCAGCT TCTTATCCAT CCACCTGCCC TTCCTCGCCA ACAGTGTTGC TCTTTTCTAC 
AATTTTGGAA AGTCTTGGAA ATCAGATCCA GGGATCATTA AAGCTACAGA AGAACAAAAG 
AAGAAGACAA TAGTTGAACT TGCAGAGACC GGCAGTCTGG ACCTCAGTAT ATTCTGCAGC 
ACCTGCTTGA TTCGGAAGCC GGTGAGGTCC AAACACTGTG GTGTGTGCAA CCGCTGTATA 
GCGAAATTTG ATCACCATTG CCCATGGGTG GGTAACTGTG TAGACTGGGG ACTTCACTGT 
GAGACCACGT ACACGAAGGA TGGCTTCTGG ACATATATTA CTCAGATTGC CACATGCTCT 
CCTTGGATGT TTTGGATGTT TCTGAACAGT GTGTTTCATT TCATGTGGGT GGCCGTGCTA 
CTCATGTGTC AGATGTACCA GATAACATGT TTAGGTATTA CTACAAATGA AAGAATGAAT 
GCCAGGAGAT ACAAGCACTT TAAAGTTACA ACAACATCTA TAGAGAGCCC ATTCAACCAT 
GGATGTGTAA GGAATATTAT AGACTTCTTT GAATTTCGAT GCTGTGGCCT CTTTCGTCCT 
GTTATCGTGG ACTGGACCAG GCAATACACA ATAGAGTATG ACCAAATATC AGGATCTGGG 
TACCAGCTGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450506.1
CDS	107..1921
Translation	MQREEGFNTKMADGPDEYDTETGCVPLLHPEEIKPQSHYNHGYGEPLGRKTHVDDYSTWDIVKATQYGIYERCRELVEAGYDVRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMVVQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHSVDPTRLLLTFNVSVNLGDKYHKNTALHWAVLAGNTTVISLLLEAGGNVDAQNVKGESALDLAKQRKNVWMINHLQEARQAKGYDNPSFLRKLKADKEFRQKVMLGTPFLVIWLVGFIADLNIDSWLIKGLMYGGVWATVQFLSKSFFDHSMHSALPLGIYLATKFWMYVTWFFWFWNDLSFLSIHLPFLANSVALFYNFGKSWKSDPGIIKATEEQKKKTIVELAETGSLDLSIFCSTCLIRKPVRSKHCGVCNRCIAKFDHHCPWVGNCVDWGLHCETTYTKDGFWTYITQIATCSPWMFWMFLNSVFHFMWVAVLLMCQMYQITCLGITTNERMNARRYKHFKVTTTSIESPFNHGCVRNIIDFFEFRCCGLFRPVIVDWTRQYTIEYDQISGSGYQLV

Target ORF information:

RefSeq Version	XM_017595017.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus zinc finger, DHHC-type containing 17 (Zdhhc17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595017.1

ORF Insert Sequence:

ATGCAGCGGG AGGAGGGATT TAACACCAAG ATGGCGGACG GCCCGGATGA GTACGATACC 
GAAACGGGCT GTGTGCCCCT TCTCCACCCG GAGGAGATCA AACCACAGAG CCATTATAAC 
CATGGGTATG GTGAGCCTCT TGGGCGGAAA ACCCATGTTG ATGACTACAG CACATGGGAC 
ATTGTCAAGG CTACACAATA CGGAATATAT GAACGCTGCC GAGAATTGGT GGAAGCAGGT 
TATGACGTAC GGCAACCGGA CAAAGAGAAC GTTACACTTC TTCACTGGGC TGCCATCAAT 
AACAGAATAG ATTTAGTCAA ATACTATATT TCGAAAGGTG CTATTGTGGA TCAACTTGGA 
GGAGACCTGA ATTCAACTCC ATTGCACTGG GCCACAAGAC AAGGCCATTT ATCCATGGTT 
GTACAACTAA TGAAATATGG TGCAGATCCC TCATTGATCG ATGGTGAAGG GTGCAGCTGT 
ATCCACTTGG CTGCTCAGTT TGGACATACC TCAATTGTTG CTTACCTTAT AGCAAAAGGA 
CAGGATGTAG ATATGATGGA TCAGAACGGA ATGACACCTT TAATGTGGGC AGCTTATAGA 
ACACATAGTG TGGATCCAAC TAGATTGCTT TTAACATTCA ATGTTTCAGT TAACCTTGGT 
GACAAGTATC ACAAAAACAC TGCTCTGCAC TGGGCAGTCC TAGCAGGGAA CACCACAGTC 
ATTAGTCTTC TTCTGGAAGC TGGAGGGAAC GTGGATGCCC AGAATGTCAA GGGCGAATCA 
GCACTTGATT TGGCAAAGCA AAGAAAAAAC GTGTGGATGA TCAACCATTT GCAGGAGGCA 
AGGCAGGCGA AGGGTTACGA CAACCCGTCC TTCCTCAGAA AGCTGAAGGC TGACAAGGAA 
TTTCGGCAGA AAGTAATGCT AGGAACTCCA TTCCTAGTGA TTTGGCTAGT TGGGTTTATA 
GCAGACCTGA ACATTGATTC TTGGCTCATT AAAGGGTTAA TGTATGGTGG TGTTTGGGCT 
ACAGTACAAT TTCTTTCCAA GTCCTTTTTT GATCATTCAA TGCACAGCGC ATTGCCCCTT 
GGGATATATT TGGCAACCAA ATTCTGGATG TATGTGACAT GGTTCTTCTG GTTTTGGAAT 
GATCTCAGCT TCTTATCCAT CCACCTGCCC TTCCTCGCCA ACAGTGTTGC TCTTTTCTAC 
AATTTTGGAA AGTCTTGGAA ATCAGATCCA GGGATCATTA AAGCTACAGA AGAACAAAAG 
AAGAAGACAA TAGTTGAACT TGCAGAGACC GGCAGTCTGG ACCTCAGTAT ATTCTGCAGC 
ACCTGCTTGA TTCGGAAGCC GGTGAGGTCC AAACACTGTG GTGTGTGCAA CCGCTGTATA 
GCGAAATTTG ATCACCATTG CCCATGGGTG GGTAACTGTG TAGACTGGGG ACTTCACTGT 
GAGACCACGT ACACGAAGGA TGGCTTCTGG ACATATATTA CTCAGATTGC CACATGCTCT 
CCTTGGATGT TTTGGATGTT TCTGAACAGT GTGTTTCATT TCATGTGGGT GGCCGTGCTA 
CTCATGTGTC AGATGTACCA GATAACATGT TTAGGTATTA CTACAAATGA AAGAATGAAT 
GCCAGGAGAT ACAAGCACTT TAAAGTTACA ACAACATCTA TAGAGAGCCC ATTCAACCAT 
GGATGTGTAA GGAATATTAT AGACTTCTTT GAATTTCGAT GCTGTGGCCT CTTTCGTCCT 
GTTATCGTGG ACTGGACCAG GCAATACACA ATAGAGTATG ACCAAATATC AGGATCTGGG 
TACCAGCTGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Golgi S-acylation machinery comprises zDHHC enzymes with major differences in substrate affinity and S-acylation activity.
Molecular biology of the cell25(24)3870-83(2014 Dec)
Lemonidis K,Gorleku OA,Sanchez-Perez MC,Grefen C,Chamberlain LH

The palmitoyl acyltransferase HIP14 shares a high proportion of interactors with huntingtin: implications for a role in the pathogenesis of Huntington's disease.
Human molecular genetics23(15)4142-60(2014 Aug)
Butland SL,Sanders SS,Schmidt ME,Riechers SP,Lin DT,Martin DD,Vaid K,Graham RK,Singaraja RR,Wanker EE,Conibear E,Hayden MR

Analysis of substrate specificity of human DHHC protein acyltransferases using a yeast expression system.
Molecular biology of the cell23(23)4543-51(2012 Dec)
Ohno Y,Kashio A,Ogata R,Ishitomi A,Yamazaki Y,Kihara A

Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein regulating insulin secretion and beta-cell apoptosis.
Proceedings of the National Academy of Sciences of the United States of America108(37)E681-8(2011 Sep)
Berchtold LA,St?rling ZM,Ortis F,Lage K,Bang-Berthelsen C,Bergholdt R,Hald J,Brorsson CA,Eizirik DL,Pociot F,Brunak S,St?rling J

Huntingtin-interacting proteins, HIP14 and HIP14L, mediate dual functions, palmitoyl acyltransferase and Mg2+ transport.
The Journal of biological chemistry283(48)33365-74(2008 Nov)
Goytain A,Hines RM,Quamme GA

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Zdhhc17 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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