Alkbh8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alkbh8
Entrez Gene ID	366783
Full Name	alkB homolog 8, tRNA methyltransferase
Synonyms	RGD1304687
General protein information	Preferred Names alkB homolog 8, tRNA methyltransferase Names alkylated DNA repair protein alkB homolog 8 alkB, alkylation repair homolog 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239751.3	XP_006239813.1	alkylated DNA repair protein alkB homolog 8 isoform X2
XM_006239752.3	XP_006239814.1	alkylated DNA repair protein alkB homolog 8 isoform X3
XM_006239749.3	XP_006239811.1	alkylated DNA repair protein alkB homolog 8 isoform X1
XM_006239750.3	XP_006239812.1	alkylated DNA repair protein alkB homolog 8 isoform X1
XM_017594262.1	XP_017449751.1	alkylated DNA repair protein alkB homolog 8 isoform X1
NM_001191909.1	NP_001178838.1	alkylated DNA repair protein alkB homolog 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	ALKBH8	NP_620130.2
Canis lupus familiaris (dog)	ALKBH8	XP_005619893.1
Bos taurus (cattle)	ALKBH8	NP_001073810.2
Gallus gallus (chicken)	ALKBH8	XP_004938885.1
Saccharomyces cerevisiae (baker's yeast)	TRM9	NP_013698.1
	ALKBH8	XP_001102856.2
Mus musculus (house mouse)	Alkbh8	NP_080579.1
Rattus norvegicus (Norway rat)	Alkbh8	NP_001178838.1
Caenorhabditis elegans (roundworm)	alkb-8	NP_497751.1
Eremothecium gossypii	AGOS_ADR181W	NP_984277.2
Neurospora crassa	NCU01705	XP_956911.1
Xenopus tropicalis (tropical clawed frog)	alkbh8	NP_001017297.2
Danio rerio (zebrafish)	alkbh8	XP_684243.5
Drosophila melanogaster (fruit fly)	CG17807	NP_611690.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011900	XP_320626.2
Schizosaccharomyces pombe (fission yeast)	trm9	NP_593681.1
Magnaporthe oryzae (rice blast fungus)	MGG_14001	XP_003716091.1
Pan troglodytes (chimpanzee)	ALKBH8	XP_522172.3
Kluyveromyces lactis	KLLA0B01023g	XP_451576.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure and RNA binding properties of the RNA recognition motif (RRM) and AlkB domains in human AlkB homolog 8 (ABH8), an enzyme catalyzing tRNA hypermodification.
Pastore C, Topalidou I, Forouhar F, Yan AC, Levy M, Hunt JF
The Journal of biological chemistry287(3)2130-43(2012 Jan)

ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.
van den Born E, V?gb? CB, Songe-M?ller L, Leihne V, Lien GF, Leszczynska G, Malkiewicz A, Krokan HE, Kirpekar F, Klungland A, Falnes P?
Nature communications2172(2011 Feb)

The AlkB domain of mammalian ABH8 catalyzes hydroxylation of 5-methoxycarbonylmethyluridine at the wobble position of tRNA.
Fu Y, Dai Q, Zhang W, Ren J, Pan T, He C
Angewandte Chemie (International ed. in English)49(47)8885-8(2010 Nov)

Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.
Fu D, Brophy JA, Chan CT, Atmore KA, Begley U, Paules RS, Dedon PC, Begley TJ, Samson LD
Molecular and cellular biology30(10)2449-59(2010 May)

Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.
Songe-M?ller L, van den Born E, Leihne V, V?gb? CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes P?, Klungland A
Molecular and cellular biology30(7)1814-27(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Alkbh8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alkbh8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08001	NM_001191909.1 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa08001	XM_006239752.3 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa29128	XM_017594262.1 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29128	XM_006239749.3 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29128	XM_006239750.3 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29129	XM_006239751.3 Latest version!	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alkbh8 ( NM_001191909.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178838.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), mRNA.

Price & Availability↑

CloneID	ORa08001
Clone ID Related Accession (Same CDS sequence)	XM_006239752.3 , NM_001191909.1
Accession Version	NM_001191909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07063132.1 and AABR07063133.1. On Jul 17, 2010 this sequence version replaced XM_345766.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGAG 
CGCAGAGTGG AAGCTCTCCA AGAAATTGCC CGTCTCCTGA GATCTGGTGG GCAGGCCCTC 
ATTTATGTCT GGGCAATGGA ACAAGAATAT AGAGATCAGA AGTCCAAGTA TCTTAAAGGA 
AACAGAATTA GCCAAGGCGA TAAAGGTGAA CTAAACAGTG CCACATCCAT GGAACAATTG 
CTAGTAAATC AAATGCCTGA GGGAGTCAGT GAAGACCCAG GATTGTCTGT CCACTCCAGT 
AATAACACCA AGGATGAAGA GTGTAAGTCA AGGAAAGTTC TTAATTCTGA ACTTCCTATT 
CATACAAATA GGACCTGTTT TCATTCCCAA GATGTGCTGG TTCCTTGGCA CCTCAAAGGA 
AAGCCTGGTA AAGACAAAGC TGTTGAACAG TCAGGCCTAG CCAGATGTCC TGACCCACGT 
CCTGTGTTTC ATCGTTATTA CCATGTTTTC TGTAATGGAG AGTTGGAAGC TTCCTGCCAG 
GCTGTGGGTG ATGTTAGCAT TCTGCAGAGC TTCTATGACC AAGGGAACTG GTGTGTTGTT 
CTTCAAAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178838.1
CDS	1..1995
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTGNQHFQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	NM_001191909.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191909.1

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGAG 
CGCAGAGTGG AAGCTCTCCA AGAAATTGCC CGTCTCCTGA GATCTGGTGG GCAGGCCCTC 
ATTTATGTCT GGGCAATGGA ACAAGAATAT AGAGATCAGA AGTCCAAGTA TCTTAAAGGA 
AACAGAATTA GCCAAGGCGA TAAAGGTGAA CTAAACAGTG CCACATCCAT GGAACAATTG 
CTAGTAAATC AAATGCCTGA GGGAGTCAGT GAAGACCCAG GATTGTCTGT CCACTCCAGT 
AATAACACCA AGGATGAAGA GTGTAAGTCA AGGAAAGTTC TTAATTCTGA ACTTCCTATT 
CATACAAATA GGACCTGTTT TCATTCCCAA GATGTGCTGG TTCCTTGGCA CCTCAAAGGA 
AAGCCTGGTA AAGACAAAGC TGTTGAACAG TCAGGCCTAG CCAGATGTCC TGACCCACGT 
CCTGTGTTTC ATCGTTATTA CCATGTTTTC TGTAATGGAG AGTTGGAAGC TTCCTGCCAG 
GCTGTGGGTG ATGTTAGCAT TCTGCAGAGC TTCTATGACC AAGGGAACTG GTGTGTTGTT 
CTTCAAAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alkbh8 ( XM_006239752.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239814.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa08001
Clone ID Related Accession (Same CDS sequence)	XM_006239752.3 , NM_001191909.1
Accession Version	XM_006239752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGAG 
CGCAGAGTGG AAGCTCTCCA AGAAATTGCC CGTCTCCTGA GATCTGGTGG GCAGGCCCTC 
ATTTATGTCT GGGCAATGGA ACAAGAATAT AGAGATCAGA AGTCCAAGTA TCTTAAAGGA 
AACAGAATTA GCCAAGGCGA TAAAGGTGAA CTAAACAGTG CCACATCCAT GGAACAATTG 
CTAGTAAATC AAATGCCTGA GGGAGTCAGT GAAGACCCAG GATTGTCTGT CCACTCCAGT 
AATAACACCA AGGATGAAGA GTGTAAGTCA AGGAAAGTTC TTAATTCTGA ACTTCCTATT 
CATACAAATA GGACCTGTTT TCATTCCCAA GATGTGCTGG TTCCTTGGCA CCTCAAAGGA 
AAGCCTGGTA AAGACAAAGC TGTTGAACAG TCAGGCCTAG CCAGATGTCC TGACCCACGT 
CCTGTGTTTC ATCGTTATTA CCATGTTTTC TGTAATGGAG AGTTGGAAGC TTCCTGCCAG 
GCTGTGGGTG ATGTTAGCAT TCTGCAGAGC TTCTATGACC AAGGGAACTG GTGTGTTGTT 
CTTCAAAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239814.1
CDS	142..2136
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTGNQHFQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	XM_006239752.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239752.3

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGAG 
CGCAGAGTGG AAGCTCTCCA AGAAATTGCC CGTCTCCTGA GATCTGGTGG GCAGGCCCTC 
ATTTATGTCT GGGCAATGGA ACAAGAATAT AGAGATCAGA AGTCCAAGTA TCTTAAAGGA 
AACAGAATTA GCCAAGGCGA TAAAGGTGAA CTAAACAGTG CCACATCCAT GGAACAATTG 
CTAGTAAATC AAATGCCTGA GGGAGTCAGT GAAGACCCAG GATTGTCTGT CCACTCCAGT 
AATAACACCA AGGATGAAGA GTGTAAGTCA AGGAAAGTTC TTAATTCTGA ACTTCCTATT 
CATACAAATA GGACCTGTTT TCATTCCCAA GATGTGCTGG TTCCTTGGCA CCTCAAAGGA 
AAGCCTGGTA AAGACAAAGC TGTTGAACAG TCAGGCCTAG CCAGATGTCC TGACCCACGT 
CCTGTGTTTC ATCGTTATTA CCATGTTTTC TGTAATGGAG AGTTGGAAGC TTCCTGCCAG 
GCTGTGGGTG ATGTTAGCAT TCTGCAGAGC TTCTATGACC AAGGGAACTG GTGTGTTGTT 
CTTCAAAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alkbh8 ( XM_006239749.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239811.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29128
Clone ID Related Accession (Same CDS sequence)	XM_006239749.3 , XM_006239750.3 , XM_017594262.1
Accession Version	XM_006239749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239811.1
CDS	142..2163
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTGNQHFQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAVSVAFPSVKERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	XM_006239749.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239749.3

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alkbh8 ( XM_006239750.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239812.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa29128
Clone ID Related Accession (Same CDS sequence)	XM_006239749.3 , XM_006239750.3 , XM_017594262.1
Accession Version	XM_006239750.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239750.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239812.1
CDS	160..2181
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTGNQHFQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAVSVAFPSVKERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	XM_006239750.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239750.3

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alkbh8 ( XM_017594262.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449751.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa29128
Clone ID Related Accession (Same CDS sequence)	XM_006239749.3 , XM_006239750.3 , XM_017594262.1
Accession Version	XM_017594262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449751.1
CDS	276..2297
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTGNQHFQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAVSVAFPSVKERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	XM_017594262.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594262.1

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGGTA ATCAGCATTT TCAAAGGTCC TTAAAACACA GAAGAGTAAA ACATTTTGGT 
TATGAATTTC ACTATGAGAA CAACACTGTG GATAAAGATA AGCCCTTACC TGGGGGTCTT 
CCTGAGATTT GCAGTAGCAT TTTGGAGAAG TTGTTGAAAG AAGGTTATAT TAAACATAAG 
CCAGACCAGT TGACCATAAA TCAGTATGAG CCTGGGCATG GAATTCCTGC CCATATTGAC 
TCGCATTCTG CTTTTGAACA TGAGATTGTT TCCCTCAGTT TGGGGTCAGC GATTGTTATG 
GATTTCAAGC ACCCAGAGGG TGTCACAGTA CAAGTTATGT TACCTCGTCG GAGTTTGCTG 
GTAATGACGG GAGAGTCTAG ATACCTTTGG ACTCATGGAA TTACACCTCG AAAATTTGAT 
ACCATCCAAG CATCTGAGCA ATTCAAAGGT GGAATAATCA ACAGTGACAT TGGAGACTTG 
ACTTTAAGCA AGAGGGGGAT GCGCACATCG TTTACGTTTA GGAAAGTGAG GCGAATACCG 
TGCAATTGTA GTTACTCCTC CGTCTGTGAC AGACAAAGGA AGGCCACACC TCCATCCCTT 
TTGGAGAGCA GTAAAGAAGC ACTGGAGCTA GAGCAAAAGC ATGTCCATCA TGTGTACGAT 
GAGATTGCAA GTCACTTCAG CAGCACGAGG CATAGCCCAT GGCCACGCAT AGTAGAGTTC 
CTGAAAGCTT TGCCAAGTGG TTCCATAGTG GCAGATATTG GATGTGGAAA TGGAAAGTAC 
CTTGGCATCA ACAAGGAGTT ATATATGATT GGGTGTGATC GTAGCCGGAA TCTTGTGGAC 
ATTTGTAGAG AGAGGCAGTT CCAAGCCTTA GTGTGCGATG CACTAGCTGT TCCTGTCAGA 
AGTGGGTCTT GTGATGCTTG TATCTCTATT GCTGTCATTC ACCACTTTGC AACTGCAGTA 
AGTGTTGCTT TTCCCTCTGT AAAAGAGCGC AGAGTGGAAG CTCTCCAAGA AATTGCCCGT 
CTCCTGAGAT CTGGTGGGCA GGCCCTCATT TATGTCTGGG CAATGGAACA AGAATATAGA 
GATCAGAAGT CCAAGTATCT TAAAGGAAAC AGAATTAGCC AAGGCGATAA AGGTGAACTA 
AACAGTGCCA CATCCATGGA ACAATTGCTA GTAAATCAAA TGCCTGAGGG AGTCAGTGAA 
GACCCAGGAT TGTCTGTCCA CTCCAGTAAT AACACCAAGG ATGAAGAGTG TAAGTCAAGG 
AAAGTTCTTA ATTCTGAACT TCCTATTCAT ACAAATAGGA CCTGTTTTCA TTCCCAAGAT 
GTGCTGGTTC CTTGGCACCT CAAAGGAAAG CCTGGTAAAG ACAAAGCTGT TGAACAGTCA 
GGCCTAGCCA GATGTCCTGA CCCACGTCCT GTGTTTCATC GTTATTACCA TGTTTTCTGT 
AATGGAGAGT TGGAAGCTTC CTGCCAGGCT GTGGGTGATG TTAGCATTCT GCAGAGCTTC 
TATGACCAAG GGAACTGGTG TGTTGTTCTT CAAAAGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alkbh8 ( XM_006239751.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239813.1 Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X4, mRNA.

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CloneID	ORa29129
Clone ID Related Accession (Same CDS sequence)	XM_006239751.3
Accession Version	XM_006239751.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alkylated DNA repair protein alkB homolog 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239751.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGTTC AAAGGTCCTT AAAACACAGA AGAGTAAAAC ATTTTGGTTA TGAATTTCAC 
TATGAGAACA ACACTGTGGA TAAAGATAAG CCCTTACCTG GGGGTCTTCC TGAGATTTGC 
AGTAGCATTT TGGAGAAGTT GTTGAAAGAA GGTTATATTA AACATAAGCC AGACCAGTTG 
ACCATAAATC AGTATGAGCC TGGGCATGGA ATTCCTGCCC ATATTGACTC GCATTCTGCT 
TTTGAACATG AGATTGTTTC CCTCAGTTTG GGGTCAGCGA TTGTTATGGA TTTCAAGCAC 
CCAGAGGGTG TCACAGTACA AGTTATGTTA CCTCGTCGGA GTTTGCTGGT AATGACGGGA 
GAGTCTAGAT ACCTTTGGAC TCATGGAATT ACACCTCGAA AATTTGATAC CATCCAAGCA 
TCTGAGCAAT TCAAAGGTGG AATAATCAAC AGTGACATTG GAGACTTGAC TTTAAGCAAG 
AGGGGGATGC GCACATCGTT TACGTTTAGG AAAGTGAGGC GAATACCGTG CAATTGTAGT 
TACTCCTCCG TCTGTGACAG ACAAAGGAAG GCCACACCTC CATCCCTTTT GGAGAGCAGT 
AAAGAAGCAC TGGAGCTAGA GCAAAAGCAT GTCCATCATG TGTACGATGA GATTGCAAGT 
CACTTCAGCA GCACGAGGCA TAGCCCATGG CCACGCATAG TAGAGTTCCT GAAAGCTTTG 
CCAAGTGGTT CCATAGTGGC AGATATTGGA TGTGGAAATG GAAAGTACCT TGGCATCAAC 
AAGGAGTTAT ATATGATTGG GTGTGATCGT AGCCGGAATC TTGTGGACAT TTGTAGAGAG 
AGGCAGTTCC AAGCCTTAGT GTGCGATGCA CTAGCTGTTC CTGTCAGAAG TGGGTCTTGT 
GATGCTTGTA TCTCTATTGC TGTCATTCAC CACTTTGCAA CTGCAGTAAG TGTTGCTTTT 
CCCTCTGTAA AAGAGCGCAG AGTGGAAGCT CTCCAAGAAA TTGCCCGTCT CCTGAGATCT 
GGTGGGCAGG CCCTCATTTA TGTCTGGGCA ATGGAACAAG AATATAGAGA TCAGAAGTCC 
AAGTATCTTA AAGGAAACAG AATTAGCCAA GGCGATAAAG GTGAACTAAA CAGTGCCACA 
TCCATGGAAC AATTGCTAGT AAATCAAATG CCTGAGGGAG TCAGTGAAGA CCCAGGATTG 
TCTGTCCACT CCAGTAATAA CACCAAGGAT GAAGAGTGTA AGTCAAGGAA AGTTCTTAAT 
TCTGAACTTC CTATTCATAC AAATAGGACC TGTTTTCATT CCCAAGATGT GCTGGTTCCT 
TGGCACCTCA AAGGAAAGCC TGGTAAAGAC AAAGCTGTTG AACAGTCAGG CCTAGCCAGA 
TGTCCTGACC CACGTCCTGT GTTTCATCGT TATTACCATG TTTTCTGTAA TGGAGAGTTG 
GAAGCTTCCT GCCAGGCTGT GGGTGATGTT AGCATTCTGC AGAGCTTCTA TGACCAAGGG 
AACTGGTGTG TTGTTCTTCA AAAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239813.1
CDS	142..2151
Translation	MNINHEGVLKLTKMEKKFLRKQSKARHVLLKHEGIQAVSYPTQSLVIANGGLGNAVSRKQLLLTLEKCGPVEALLMPPNKPYAFVIFQTIEESKKAYSILNGKEIIDDLGQKIFLYLNFVEKAQWKKLGLQALPPGLLVVEEIISSEEEKMLLESVNWTEDTVQRSLKHRRVKHFGYEFHYENNTVDKDKPLPGGLPEICSSILEKLLKEGYIKHKPDQLTINQYEPGHGIPAHIDSHSAFEHEIVSLSLGSAIVMDFKHPEGVTVQVMLPRRSLLVMTGESRYLWTHGITPRKFDTIQASEQFKGGIINSDIGDLTLSKRGMRTSFTFRKVRRIPCNCSYSSVCDRQRKATPPSLLESSKEALELEQKHVHHVYDEIASHFSSTRHSPWPRIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSRNLVDICRERQFQALVCDALAVPVRSGSCDACISIAVIHHFATAVSVAFPSVKERRVEALQEIARLLRSGGQALIYVWAMEQEYRDQKSKYLKGNRISQGDKGELNSATSMEQLLVNQMPEGVSEDPGLSVHSSNNTKDEECKSRKVLNSELPIHTNRTCFHSQDVLVPWHLKGKPGKDKAVEQSGLARCPDPRPVFHRYYHVFCNGELEASCQAVGDVSILQSFYDQGNWCVVLQKV

Target ORF information:

RefSeq Version	XM_006239751.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alkB homolog 8, tRNA methyltransferase (Alkbh8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239751.3

ORF Insert Sequence:

ATGAATATCA ATCATGAAGG TGTTTTGAAA CTCACTAAAA TGGAGAAGAA GTTCTTAAGG 
AAACAGAGTA AAGCCAGACA TGTGTTGCTG AAACATGAAG GCATCCAGGC AGTCTCTTAT 
CCCACTCAGA GCCTGGTTAT TGCCAATGGT GGTCTGGGTA ATGCTGTGAG TAGGAAACAA 
CTGCTTTTGA CTTTAGAGAA GTGTGGACCT GTGGAAGCCC TCCTCATGCC ACCTAATAAG 
CCATATGCAT TTGTCATATT CCAAACTATT GAAGAATCCA AGAAAGCCTA TTCTATTCTC 
AATGGAAAAG AAATCATTGA TGATTTGGGG CAGAAGATCT TTCTGTACTT GAATTTTGTT 
GAAAAAGCAC AATGGAAAAA ATTGGGACTT CAAGCCTTAC CTCCAGGCCT TTTGGTGGTA 
GAAGAAATTA TTTCTTCTGA GGAGGAGAAA ATGCTTTTGG AAAGTGTTAA TTGGACAGAA 
GATACAGTTC AAAGGTCCTT AAAACACAGA AGAGTAAAAC ATTTTGGTTA TGAATTTCAC 
TATGAGAACA ACACTGTGGA TAAAGATAAG CCCTTACCTG GGGGTCTTCC TGAGATTTGC 
AGTAGCATTT TGGAGAAGTT GTTGAAAGAA GGTTATATTA AACATAAGCC AGACCAGTTG 
ACCATAAATC AGTATGAGCC TGGGCATGGA ATTCCTGCCC ATATTGACTC GCATTCTGCT 
TTTGAACATG AGATTGTTTC CCTCAGTTTG GGGTCAGCGA TTGTTATGGA TTTCAAGCAC 
CCAGAGGGTG TCACAGTACA AGTTATGTTA CCTCGTCGGA GTTTGCTGGT AATGACGGGA 
GAGTCTAGAT ACCTTTGGAC TCATGGAATT ACACCTCGAA AATTTGATAC CATCCAAGCA 
TCTGAGCAAT TCAAAGGTGG AATAATCAAC AGTGACATTG GAGACTTGAC TTTAAGCAAG 
AGGGGGATGC GCACATCGTT TACGTTTAGG AAAGTGAGGC GAATACCGTG CAATTGTAGT 
TACTCCTCCG TCTGTGACAG ACAAAGGAAG GCCACACCTC CATCCCTTTT GGAGAGCAGT 
AAAGAAGCAC TGGAGCTAGA GCAAAAGCAT GTCCATCATG TGTACGATGA GATTGCAAGT 
CACTTCAGCA GCACGAGGCA TAGCCCATGG CCACGCATAG TAGAGTTCCT GAAAGCTTTG 
CCAAGTGGTT CCATAGTGGC AGATATTGGA TGTGGAAATG GAAAGTACCT TGGCATCAAC 
AAGGAGTTAT ATATGATTGG GTGTGATCGT AGCCGGAATC TTGTGGACAT TTGTAGAGAG 
AGGCAGTTCC AAGCCTTAGT GTGCGATGCA CTAGCTGTTC CTGTCAGAAG TGGGTCTTGT 
GATGCTTGTA TCTCTATTGC TGTCATTCAC CACTTTGCAA CTGCAGTAAG TGTTGCTTTT 
CCCTCTGTAA AAGAGCGCAG AGTGGAAGCT CTCCAAGAAA TTGCCCGTCT CCTGAGATCT 
GGTGGGCAGG CCCTCATTTA TGTCTGGGCA ATGGAACAAG AATATAGAGA TCAGAAGTCC 
AAGTATCTTA AAGGAAACAG AATTAGCCAA GGCGATAAAG GTGAACTAAA CAGTGCCACA 
TCCATGGAAC AATTGCTAGT AAATCAAATG CCTGAGGGAG TCAGTGAAGA CCCAGGATTG 
TCTGTCCACT CCAGTAATAA CACCAAGGAT GAAGAGTGTA AGTCAAGGAA AGTTCTTAAT 
TCTGAACTTC CTATTCATAC AAATAGGACC TGTTTTCATT CCCAAGATGT GCTGGTTCCT 
TGGCACCTCA AAGGAAAGCC TGGTAAAGAC AAAGCTGTTG AACAGTCAGG CCTAGCCAGA 
TGTCCTGACC CACGTCCTGT GTTTCATCGT TATTACCATG TTTTCTGTAA TGGAGAGTTG 
GAAGCTTCCT GCCAGGCTGT GGGTGATGTT AGCATTCTGC AGAGCTTCTA TGACCAAGGG 
AACTGGTGTG TTGTTCTTCA AAAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure and RNA binding properties of the RNA recognition motif (RRM) and AlkB domains in human AlkB homolog 8 (ABH8), an enzyme catalyzing tRNA hypermodification.
The Journal of biological chemistry287(3)2130-43(2012 Jan)
Pastore C,Topalidou I,Forouhar F,Yan AC,Levy M,Hunt JF

ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA.
Nature communications2172(2011 Feb)
van den Born E,V?gb? CB,Songe-M?ller L,Leihne V,Lien GF,Leszczynska G,Malkiewicz A,Krokan HE,Kirpekar F,Klungland A,Falnes P?

The AlkB domain of mammalian ABH8 catalyzes hydroxylation of 5-methoxycarbonylmethyluridine at the wobble position of tRNA.
Angewandte Chemie (International ed. in English)49(47)8885-8(2010 Nov)
Fu Y,Dai Q,Zhang W,Ren J,Pan T,He C

Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival.
Molecular and cellular biology30(10)2449-59(2010 May)
Fu D,Brophy JA,Chan CT,Atmore KA,Begley U,Paules RS,Dedon PC,Begley TJ,Samson LD

Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding.
Molecular and cellular biology30(7)1814-27(2010 Apr)
Songe-M?ller L,van den Born E,Leihne V,V?gb? CB,Kristoffersen T,Krokan HE,Kirpekar F,Falnes P?,Klungland A

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Alkbh8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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