Col16a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col16a1
Entrez Gene ID	366474
Full Name	collagen type XVI alpha 1 chain
General protein information	Preferred Names collagen type XVI alpha 1 chain Names collagen alpha-1(XVI) chain collagen, type XVI, alpha 1 procollagen, type XVI, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_006238948.3	XP_006239010.1	collagen alpha-1(XVI) chain isoform X3
XM_006238953.3	XP_006239015.1	collagen alpha-1(XVI) chain isoform X6
XM_006238946.3	XP_006239008.1	collagen alpha-1(XVI) chain isoform X1
XM_006238950.3	XP_006239012.1	collagen alpha-1(XVI) chain isoform X4
XM_006238951.3	XP_006239013.1	collagen alpha-1(XVI) chain isoform X5
NM_001302967.1	NP_001289896.1	collagen alpha-1(XVI) chain precursor
XM_008764158.2	XP_008762380.1	collagen alpha-1(XVI) chain isoform X1
XM_006238947.3	XP_006239009.1	collagen alpha-1(XVI) chain isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	COL16A1	NP_001847.3
Mus musculus (house mouse)	Col16a1	NP_082542.3
Rattus norvegicus (Norway rat)	Col16a1	NP_001015033.2
Xenopus tropicalis (tropical clawed frog)	LOC100497018	XP_004919282.1
Pan troglodytes (chimpanzee)	COL16A1	XP_513270.4
Gallus gallus (chicken)	COL16A1	XP_003642629.2
	COL16A1	XP_001100182.1
Canis lupus familiaris (dog)	COL16A1	XP_544444.3
Bos taurus (cattle)	COL16A1	XP_005203115.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Collagen XVI induces expression of MMP9 via modulation of AP-1 transcription factors and facilitates invasion of oral squamous cell carcinoma.
Bedal KB, Gr?ssel S, Oefner PJ, Reinders J, Reichert TE, Bauer R
PloS one9(1)e86777(2014)

Difference in expression of hepatic microRNAs miR-29c, miR-34a, miR-155, and miR-200b is associated with strain-specific susceptibility to dietary nonalcoholic steatohepatitis in mice.
Pogribny IP, Starlard-Davenport A, Tryndyak VP, Han T, Ross SA, Rusyn I, Beland FA
Laboratory investigation; a journal of technical methods and pathology90(10)1437-46(2010 Oct)

Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains.
Eble JA, Kassner A, Niland S, M?rgelin M, Grifka J, Gr?ssel S
The Journal of biological chemistry281(35)25745-56(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Col16a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col16a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa28646	NM_001302967.1 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28641	XM_006238946.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28641	XM_008764158.2 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28642	XM_006238947.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28643	XM_006238948.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28645	XM_006238950.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28646	XM_006238951.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa28648	XM_006238953.3 Latest version!	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col16a1 ( NM_001302967.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001289896.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), mRNA.

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CloneID	ORa28646
Clone ID Related Accession (Same CDS sequence)	XM_006238951.3 , NM_001302967.1
Accession Version	NM_001302967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07050014.1 and AABR07050015.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GTCTTCAAGG GGAGCGAGGC 
CCCACAGGCC TGACTGGAGA CAAGGGAGAA CCGGGTCCCC CAGGACAGCC TGGCTACCCG 
GGTGCCATGG GTCCCCCAGG ACTGCCTGGC ATCAAGGGAG AAAGAGGATA TGCTGGCCCT 
GCGGGAGAGA AGGGGGAGTC GGGCCCACCA GGATCTGAAG GCCTTCCAGG TCCCCAAGGT 
CCAGCGGGTC CCCGAGGAGA GCGAGGTCCC CAAGGGAGCT CGGGAGAGAA GGGTGATCAG 
GGATTTCAAG GCCAGCCAGG TTTCCCTGGC CCACCGGGTC CCCCGGGATT CCCAGGCAAA 
GCTGGAGCTC CTGGCCCACC TGGCCCCCAA GCAGAGAAGG GCAGTGAAGG GATGCGAGGT 
CCCTCAGGCT TGCCTGGTTC CCCTGGACCA CCAGGCCCTC CTGGGATTCA GGGCCCTGCT 
GGACTGGATG GACTGGATGG GAAAGACGGC AAACCGGGCC TGAGGGGGGA CCCAGGTCCC 
GCTGGCCCTC CTGGACTCAT GGGACCTCCA GGTTTCAAAG GGAAAACAGG GCATCCTGGT 
CTCCCTGGAC CTAAGGGTGA CTGTGGAAAA CCAGGTCCTC CTGGCAGCAG TGGCCGGCCA 
GGTGCAGAGG GTGAGCCCGG TGCCATGGGA CCCCAGGGAC GACCAGGACC TCCAGGGCAT 
CTTGGGCCAC AGGGGCCTCC AGGCCAGCCA GGTCCACCTG GGCTCTCCAC CATGGGCCTG 
AAAGGAGATC GGGGATCCCC TGGAGAAAGG GGCTTGGCCG GCCTCCCAGG CCAGCCTGGC 
ACACCCGGAC ACCCCGGCCC TCCGGGTGAA CCTGGTTCGG ATGGTGCAGC TGGCAAAGAG 
GGACCTCCAG GAAAGCAGGG ACTCTATGGA CCCCCCGGCC CAAAGGGTGA CCCAGGACCT 
GCAGGACAGA AAGGCCAAGC AGGAGAAAAG GGAAGATCTG GCATGCCTGG GGGCCCTGGC 
AAGAGTGGCT CCATGGGACC GATTGGACCA CCGGGTCCTG CGGGAGAGAG AGGCCACCCT 
GGGTCCCCAG GGCCTGCTGG GAACCCTGGA TTGCCTGGTT TGCCAGGCTC TATGGGAGAC 
ATGGTGAATT ATGATGATAT CAAGAGGTTT ATCAGACAAG AGATCATTAA ACTGTTTGAT 
GAGAGGATGG CTTACTACAC CTCTAGGATG CAGTTCCCCA TGGAGGTGGC AGCAGCTCCA 
GGCCGGCCAG GGCCTCCAGG GAAGGATGGC GCCCCAGGGC GGCCAGGTGC TCCAGGGTCA 
CCTGGGCTCC CTGGTCAGAT AGGCCGAGAA GGACGCCAGG GTTTGCCAGG AATGAGAGGA 
TTGCCTGGTA CCAAAGGTGA TAAGGGAGAC ATTGGTGTTG GCATTGCAGG AGAAAATGGT 
CTCCCTGGCC CTCCAGGTCC TCAAGGGCCT CCAGGTTATG GCAAGATGGG TGCAACCGGG 
CCAATGGGCC AGCAAGGCAT TCCTGGGATC CCTGGCCCCC CGGGGCCCAT GGGCCAGCCA 
GGGAAGGCAG GCCACTGCAA CCCCTCAGAC TGTTTTGGGG CCATGCCAAT GGAGCAGCAG 
TACCCGCCCA TAAAAAGCAT GAAGGGGCCT TTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289896.1
CDS	239..5017
Translation	MLVSWAPGLWVLGLWATFSHGTNIGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	NM_001302967.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302967.1

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GTCTTCAAGG GGAGCGAGGC 
CCCACAGGCC TGACTGGAGA CAAGGGAGAA CCGGGTCCCC CAGGACAGCC TGGCTACCCG 
GGTGCCATGG GTCCCCCAGG ACTGCCTGGC ATCAAGGGAG AAAGAGGATA TGCTGGCCCT 
GCGGGAGAGA AGGGGGAGTC GGGCCCACCA GGATCTGAAG GCCTTCCAGG TCCCCAAGGT 
CCAGCGGGTC CCCGAGGAGA GCGAGGTCCC CAAGGGAGCT CGGGAGAGAA GGGTGATCAG 
GGATTTCAAG GCCAGCCAGG TTTCCCTGGC CCACCGGGTC CCCCGGGATT CCCAGGCAAA 
GCTGGAGCTC CTGGCCCACC TGGCCCCCAA GCAGAGAAGG GCAGTGAAGG GATGCGAGGT 
CCCTCAGGCT TGCCTGGTTC CCCTGGACCA CCAGGCCCTC CTGGGATTCA GGGCCCTGCT 
GGACTGGATG GACTGGATGG GAAAGACGGC AAACCGGGCC TGAGGGGGGA CCCAGGTCCC 
GCTGGCCCTC CTGGACTCAT GGGACCTCCA GGTTTCAAAG GGAAAACAGG GCATCCTGGT 
CTCCCTGGAC CTAAGGGTGA CTGTGGAAAA CCAGGTCCTC CTGGCAGCAG TGGCCGGCCA 
GGTGCAGAGG GTGAGCCCGG TGCCATGGGA CCCCAGGGAC GACCAGGACC TCCAGGGCAT 
CTTGGGCCAC AGGGGCCTCC AGGCCAGCCA GGTCCACCTG GGCTCTCCAC CATGGGCCTG 
AAAGGAGATC GGGGATCCCC TGGAGAAAGG GGCTTGGCCG GCCTCCCAGG CCAGCCTGGC 
ACACCCGGAC ACCCCGGCCC TCCGGGTGAA CCTGGTTCGG ATGGTGCAGC TGGCAAAGAG 
GGACCTCCAG GAAAGCAGGG ACTCTATGGA CCCCCCGGCC CAAAGGGTGA CCCAGGACCT 
GCAGGACAGA AAGGCCAAGC AGGAGAAAAG GGAAGATCTG GCATGCCTGG GGGCCCTGGC 
AAGAGTGGCT CCATGGGACC GATTGGACCA CCGGGTCCTG CGGGAGAGAG AGGCCACCCT 
GGGTCCCCAG GGCCTGCTGG GAACCCTGGA TTGCCTGGTT TGCCAGGCTC TATGGGAGAC 
ATGGTGAATT ATGATGATAT CAAGAGGTTT ATCAGACAAG AGATCATTAA ACTGTTTGAT 
GAGAGGATGG CTTACTACAC CTCTAGGATG CAGTTCCCCA TGGAGGTGGC AGCAGCTCCA 
GGCCGGCCAG GGCCTCCAGG GAAGGATGGC GCCCCAGGGC GGCCAGGTGC TCCAGGGTCA 
CCTGGGCTCC CTGGTCAGAT AGGCCGAGAA GGACGCCAGG GTTTGCCAGG AATGAGAGGA 
TTGCCTGGTA CCAAAGGTGA TAAGGGAGAC ATTGGTGTTG GCATTGCAGG AGAAAATGGT 
CTCCCTGGCC CTCCAGGTCC TCAAGGGCCT CCAGGTTATG GCAAGATGGG TGCAACCGGG 
CCAATGGGCC AGCAAGGCAT TCCTGGGATC CCTGGCCCCC CGGGGCCCAT GGGCCAGCCA 
GGGAAGGCAG GCCACTGCAA CCCCTCAGAC TGTTTTGGGG CCATGCCAAT GGAGCAGCAG 
TACCCGCCCA TAAAAAGCAT GAAGGGGCCT TTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238946.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239008.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X1, mRNA.

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CloneID	ORa28641
Clone ID Related Accession (Same CDS sequence)	XM_006238946.3 , XM_008764158.2
Accession Version	XM_006238946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGC 
CCCCCAGGTT TTCCTGGTGC TGTTGGCTCC CCCGGATTGC CTGGTCTTCA AGGGGAGCGA 
GGCCCCACAG GCCTGACTGG AGACAAGGGA GAACCGGGTC CCCCAGGACA GCCTGGCTAC 
CCGGGTGCCA TGGGTCCCCC AGGACTGCCT GGCATCAAGG GAGAAAGAGG ATATGCTGGC 
CCTGCGGGAG AGAAGGGGGA GTCGGGCCCA CCAGGATCTG AAGGCCTTCC AGGTCCCCAA 
GGTCCAGCGG GTCCCCGAGG AGAGCGAGGT CCCCAAGGGA GCTCGGGAGA GAAGGGTGAT 
CAGGGATTTC AAGGCCAGCC AGGTTTCCCT GGCCCACCGG GTCCCCCGGG ATTCCCAGGC 
AAAGCTGGAG CTCCTGGCCC ACCTGGCCCC CAAGCAGAGA AGGGCAGTGA AGGGATGCGA 
GGTCCCTCAG GCTTGCCTGG TTCCCCTGGA CCACCAGGCC CTCCTGGGAT TCAGGGCCCT 
GCTGGACTGG ATGGACTGGA TGGGAAAGAC GGCAAACCGG GCCTGAGGGG GGACCCAGGT 
CCCGCTGGCC CTCCTGGACT CATGGGACCT CCAGGTTTCA AAGGGAAAAC AGGGCATCCT 
GGTCTCCCTG GACCTAAGGG TGACTGTGGA AAACCAGGTC CTCCTGGCAG CAGTGGCCGG 
CCAGGTGCAG AGGGTGAGCC CGGTGCCATG GGACCCCAGG GACGACCAGG ACCTCCAGGG 
CATCTTGGGC CACAGGGGCC TCCAGGCCAG CCAGGTCCAC CTGGGCTCTC CACCATGGGC 
CTGAAAGGAG ATCGGGGATC CCCTGGAGAA AGGGGCTTGG CCGGCCTCCC AGGCCAGCCT 
GGCACACCCG GACACCCCGG CCCTCCGGGT GAACCTGGTT CGGATGGTGC AGCTGGCAAA 
GAGGGACCTC CAGGAAAGCA GGGACTCTAT GGACCCCCCG GCCCAAAGGG TGACCCAGGA 
CCTGCAGGAC AGAAAGGCCA AGCAGGAGAA AAGGGAAGAT CTGGCATGCC TGGGGGCCCT 
GGCAAGAGTG GCTCCATGGG ACCGATTGGA CCACCGGGTC CTGCGGGAGA GAGAGGCCAC 
CCTGGGTCCC CAGGGCCTGC TGGGAACCCT GGATTGCCTG GTTTGCCAGG CTCTATGGGA 
GACATGGTGA ATTATGATGA TATCAAGAGG TTTATCAGAC AAGAGATCAT TAAACTGTTT 
GATGAGAGGA TGGCTTACTA CACCTCTAGG ATGCAGTTCC CCATGGAGGT GGCAGCAGCT 
CCAGGCCGGC CAGGGCCTCC AGGGAAGGAT GGCGCCCCAG GGCGGCCAGG TGCTCCAGGG 
TCACCTGGGC TCCCTGGTCA GATAGGCCGA GAAGGACGCC AGGGTTTGCC AGGAATGAGA 
GGATTGCCTG GTACCAAAGG TGATAAGGGA GACATTGGTG TTGGCATTGC AGGAGAAAAT 
GGTCTCCCTG GCCCTCCAGG TCCTCAAGGG CCTCCAGGTT ATGGCAAGAT GGGTGCAACC 
GGGCCAATGG GCCAGCAAGG CATTCCTGGG ATCCCTGGCC CCCCGGGGCC CATGGGCCAG 
CCAGGGAAGG CAGGCCACTG CAACCCCTCA GACTGTTTTG GGGCCATGCC AATGGAGCAG 
CAGTACCCGC CCATAAAAAG CATGAAGGGG CCTTTTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239008.1
CDS	356..5197
Translation	MLVSWAPGLWVLGLWATFSHGTNIGANSVPGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGPPGFPGAVGSPGLPGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238946.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238946.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGC 
CCCCCAGGTT TTCCTGGTGC TGTTGGCTCC CCCGGATTGC CTGGTCTTCA AGGGGAGCGA 
GGCCCCACAG GCCTGACTGG AGACAAGGGA GAACCGGGTC CCCCAGGACA GCCTGGCTAC 
CCGGGTGCCA TGGGTCCCCC AGGACTGCCT GGCATCAAGG GAGAAAGAGG ATATGCTGGC 
CCTGCGGGAG AGAAGGGGGA GTCGGGCCCA CCAGGATCTG AAGGCCTTCC AGGTCCCCAA 
GGTCCAGCGG GTCCCCGAGG AGAGCGAGGT CCCCAAGGGA GCTCGGGAGA GAAGGGTGAT 
CAGGGATTTC AAGGCCAGCC AGGTTTCCCT GGCCCACCGG GTCCCCCGGG ATTCCCAGGC 
AAAGCTGGAG CTCCTGGCCC ACCTGGCCCC CAAGCAGAGA AGGGCAGTGA AGGGATGCGA 
GGTCCCTCAG GCTTGCCTGG TTCCCCTGGA CCACCAGGCC CTCCTGGGAT TCAGGGCCCT 
GCTGGACTGG ATGGACTGGA TGGGAAAGAC GGCAAACCGG GCCTGAGGGG GGACCCAGGT 
CCCGCTGGCC CTCCTGGACT CATGGGACCT CCAGGTTTCA AAGGGAAAAC AGGGCATCCT 
GGTCTCCCTG GACCTAAGGG TGACTGTGGA AAACCAGGTC CTCCTGGCAG CAGTGGCCGG 
CCAGGTGCAG AGGGTGAGCC CGGTGCCATG GGACCCCAGG GACGACCAGG ACCTCCAGGG 
CATCTTGGGC CACAGGGGCC TCCAGGCCAG CCAGGTCCAC CTGGGCTCTC CACCATGGGC 
CTGAAAGGAG ATCGGGGATC CCCTGGAGAA AGGGGCTTGG CCGGCCTCCC AGGCCAGCCT 
GGCACACCCG GACACCCCGG CCCTCCGGGT GAACCTGGTT CGGATGGTGC AGCTGGCAAA 
GAGGGACCTC CAGGAAAGCA GGGACTCTAT GGACCCCCCG GCCCAAAGGG TGACCCAGGA 
CCTGCAGGAC AGAAAGGCCA AGCAGGAGAA AAGGGAAGAT CTGGCATGCC TGGGGGCCCT 
GGCAAGAGTG GCTCCATGGG ACCGATTGGA CCACCGGGTC CTGCGGGAGA GAGAGGCCAC 
CCTGGGTCCC CAGGGCCTGC TGGGAACCCT GGATTGCCTG GTTTGCCAGG CTCTATGGGA 
GACATGGTGA ATTATGATGA TATCAAGAGG TTTATCAGAC AAGAGATCAT TAAACTGTTT 
GATGAGAGGA TGGCTTACTA CACCTCTAGG ATGCAGTTCC CCATGGAGGT GGCAGCAGCT 
CCAGGCCGGC CAGGGCCTCC AGGGAAGGAT GGCGCCCCAG GGCGGCCAGG TGCTCCAGGG 
TCACCTGGGC TCCCTGGTCA GATAGGCCGA GAAGGACGCC AGGGTTTGCC AGGAATGAGA 
GGATTGCCTG GTACCAAAGG TGATAAGGGA GACATTGGTG TTGGCATTGC AGGAGAAAAT 
GGTCTCCCTG GCCCTCCAGG TCCTCAAGGG CCTCCAGGTT ATGGCAAGAT GGGTGCAACC 
GGGCCAATGG GCCAGCAAGG CATTCCTGGG ATCCCTGGCC CCCCGGGGCC CATGGGCCAG 
CCAGGGAAGG CAGGCCACTG CAACCCCTCA GACTGTTTTG GGGCCATGCC AATGGAGCAG 
CAGTACCCGC CCATAAAAAG CATGAAGGGG CCTTTTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_008764158.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762380.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa28641
Clone ID Related Accession (Same CDS sequence)	XM_006238946.3 , XM_008764158.2
Accession Version	XM_008764158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGC 
CCCCCAGGTT TTCCTGGTGC TGTTGGCTCC CCCGGATTGC CTGGTCTTCA AGGGGAGCGA 
GGCCCCACAG GCCTGACTGG AGACAAGGGA GAACCGGGTC CCCCAGGACA GCCTGGCTAC 
CCGGGTGCCA TGGGTCCCCC AGGACTGCCT GGCATCAAGG GAGAAAGAGG ATATGCTGGC 
CCTGCGGGAG AGAAGGGGGA GTCGGGCCCA CCAGGATCTG AAGGCCTTCC AGGTCCCCAA 
GGTCCAGCGG GTCCCCGAGG AGAGCGAGGT CCCCAAGGGA GCTCGGGAGA GAAGGGTGAT 
CAGGGATTTC AAGGCCAGCC AGGTTTCCCT GGCCCACCGG GTCCCCCGGG ATTCCCAGGC 
AAAGCTGGAG CTCCTGGCCC ACCTGGCCCC CAAGCAGAGA AGGGCAGTGA AGGGATGCGA 
GGTCCCTCAG GCTTGCCTGG TTCCCCTGGA CCACCAGGCC CTCCTGGGAT TCAGGGCCCT 
GCTGGACTGG ATGGACTGGA TGGGAAAGAC GGCAAACCGG GCCTGAGGGG GGACCCAGGT 
CCCGCTGGCC CTCCTGGACT CATGGGACCT CCAGGTTTCA AAGGGAAAAC AGGGCATCCT 
GGTCTCCCTG GACCTAAGGG TGACTGTGGA AAACCAGGTC CTCCTGGCAG CAGTGGCCGG 
CCAGGTGCAG AGGGTGAGCC CGGTGCCATG GGACCCCAGG GACGACCAGG ACCTCCAGGG 
CATCTTGGGC CACAGGGGCC TCCAGGCCAG CCAGGTCCAC CTGGGCTCTC CACCATGGGC 
CTGAAAGGAG ATCGGGGATC CCCTGGAGAA AGGGGCTTGG CCGGCCTCCC AGGCCAGCCT 
GGCACACCCG GACACCCCGG CCCTCCGGGT GAACCTGGTT CGGATGGTGC AGCTGGCAAA 
GAGGGACCTC CAGGAAAGCA GGGACTCTAT GGACCCCCCG GCCCAAAGGG TGACCCAGGA 
CCTGCAGGAC AGAAAGGCCA AGCAGGAGAA AAGGGAAGAT CTGGCATGCC TGGGGGCCCT 
GGCAAGAGTG GCTCCATGGG ACCGATTGGA CCACCGGGTC CTGCGGGAGA GAGAGGCCAC 
CCTGGGTCCC CAGGGCCTGC TGGGAACCCT GGATTGCCTG GTTTGCCAGG CTCTATGGGA 
GACATGGTGA ATTATGATGA TATCAAGAGG TTTATCAGAC AAGAGATCAT TAAACTGTTT 
GATGAGAGGA TGGCTTACTA CACCTCTAGG ATGCAGTTCC CCATGGAGGT GGCAGCAGCT 
CCAGGCCGGC CAGGGCCTCC AGGGAAGGAT GGCGCCCCAG GGCGGCCAGG TGCTCCAGGG 
TCACCTGGGC TCCCTGGTCA GATAGGCCGA GAAGGACGCC AGGGTTTGCC AGGAATGAGA 
GGATTGCCTG GTACCAAAGG TGATAAGGGA GACATTGGTG TTGGCATTGC AGGAGAAAAT 
GGTCTCCCTG GCCCTCCAGG TCCTCAAGGG CCTCCAGGTT ATGGCAAGAT GGGTGCAACC 
GGGCCAATGG GCCAGCAAGG CATTCCTGGG ATCCCTGGCC CCCCGGGGCC CATGGGCCAG 
CCAGGGAAGG CAGGCCACTG CAACCCCTCA GACTGTTTTG GGGCCATGCC AATGGAGCAG 
CAGTACCCGC CCATAAAAAG CATGAAGGGG CCTTTTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762380.1
CDS	458..5299
Translation	MLVSWAPGLWVLGLWATFSHGTNIGANSVPGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGPPGFPGAVGSPGLPGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_008764158.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764158.2

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGC 
CCCCCAGGTT TTCCTGGTGC TGTTGGCTCC CCCGGATTGC CTGGTCTTCA AGGGGAGCGA 
GGCCCCACAG GCCTGACTGG AGACAAGGGA GAACCGGGTC CCCCAGGACA GCCTGGCTAC 
CCGGGTGCCA TGGGTCCCCC AGGACTGCCT GGCATCAAGG GAGAAAGAGG ATATGCTGGC 
CCTGCGGGAG AGAAGGGGGA GTCGGGCCCA CCAGGATCTG AAGGCCTTCC AGGTCCCCAA 
GGTCCAGCGG GTCCCCGAGG AGAGCGAGGT CCCCAAGGGA GCTCGGGAGA GAAGGGTGAT 
CAGGGATTTC AAGGCCAGCC AGGTTTCCCT GGCCCACCGG GTCCCCCGGG ATTCCCAGGC 
AAAGCTGGAG CTCCTGGCCC ACCTGGCCCC CAAGCAGAGA AGGGCAGTGA AGGGATGCGA 
GGTCCCTCAG GCTTGCCTGG TTCCCCTGGA CCACCAGGCC CTCCTGGGAT TCAGGGCCCT 
GCTGGACTGG ATGGACTGGA TGGGAAAGAC GGCAAACCGG GCCTGAGGGG GGACCCAGGT 
CCCGCTGGCC CTCCTGGACT CATGGGACCT CCAGGTTTCA AAGGGAAAAC AGGGCATCCT 
GGTCTCCCTG GACCTAAGGG TGACTGTGGA AAACCAGGTC CTCCTGGCAG CAGTGGCCGG 
CCAGGTGCAG AGGGTGAGCC CGGTGCCATG GGACCCCAGG GACGACCAGG ACCTCCAGGG 
CATCTTGGGC CACAGGGGCC TCCAGGCCAG CCAGGTCCAC CTGGGCTCTC CACCATGGGC 
CTGAAAGGAG ATCGGGGATC CCCTGGAGAA AGGGGCTTGG CCGGCCTCCC AGGCCAGCCT 
GGCACACCCG GACACCCCGG CCCTCCGGGT GAACCTGGTT CGGATGGTGC AGCTGGCAAA 
GAGGGACCTC CAGGAAAGCA GGGACTCTAT GGACCCCCCG GCCCAAAGGG TGACCCAGGA 
CCTGCAGGAC AGAAAGGCCA AGCAGGAGAA AAGGGAAGAT CTGGCATGCC TGGGGGCCCT 
GGCAAGAGTG GCTCCATGGG ACCGATTGGA CCACCGGGTC CTGCGGGAGA GAGAGGCCAC 
CCTGGGTCCC CAGGGCCTGC TGGGAACCCT GGATTGCCTG GTTTGCCAGG CTCTATGGGA 
GACATGGTGA ATTATGATGA TATCAAGAGG TTTATCAGAC AAGAGATCAT TAAACTGTTT 
GATGAGAGGA TGGCTTACTA CACCTCTAGG ATGCAGTTCC CCATGGAGGT GGCAGCAGCT 
CCAGGCCGGC CAGGGCCTCC AGGGAAGGAT GGCGCCCCAG GGCGGCCAGG TGCTCCAGGG 
TCACCTGGGC TCCCTGGTCA GATAGGCCGA GAAGGACGCC AGGGTTTGCC AGGAATGAGA 
GGATTGCCTG GTACCAAAGG TGATAAGGGA GACATTGGTG TTGGCATTGC AGGAGAAAAT 
GGTCTCCCTG GCCCTCCAGG TCCTCAAGGG CCTCCAGGTT ATGGCAAGAT GGGTGCAACC 
GGGCCAATGG GCCAGCAAGG CATTCCTGGG ATCCCTGGCC CCCCGGGGCC CATGGGCCAG 
CCAGGGAAGG CAGGCCACTG CAACCCCTCA GACTGTTTTG GGGCCATGCC AATGGAGCAG 
CAGTACCCGC CCATAAAAAG CATGAAGGGG CCTTTTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238947.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239009.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X3, mRNA.

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CloneID	ORa28642
Clone ID Related Accession (Same CDS sequence)	XM_006238947.3
Accession Version	XM_006238947.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238947.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GCCCCCCAGG TTTTCCTGGT 
GCTGTTGGCT CCCCCGGATT GCCTGGTCTT CAAGGGGAGC GAGGCCCCAC AGGCCTGACT 
GGAGACAAGG GAGAACCGGG TCCCCCAGGA CAGCCTGGCT ACCCGGGTGC CATGGGTCCC 
CCAGGACTGC CTGGCATCAA GGGAGAAAGA GGATATGCTG GCCCTGCGGG AGAGAAGGGG 
GAGTCGGGCC CACCAGGATC TGAAGGCCTT CCAGGTCCCC AAGGTCCAGC GGGTCCCCGA 
GGAGAGCGAG GTCCCCAAGG GAGCTCGGGA GAGAAGGGTG ATCAGGGATT TCAAGGCCAG 
CCAGGTTTCC CTGGCCCACC GGGTCCCCCG GGATTCCCAG GCAAAGCTGG AGCTCCTGGC 
CCACCTGGCC CCCAAGCAGA GAAGGGCAGT GAAGGGATGC GAGGTCCCTC AGGCTTGCCT 
GGTTCCCCTG GACCACCAGG CCCTCCTGGG ATTCAGGGCC CTGCTGGACT GGATGGACTG 
GATGGGAAAG ACGGCAAACC GGGCCTGAGG GGGGACCCAG GTCCCGCTGG CCCTCCTGGA 
CTCATGGGAC CTCCAGGTTT CAAAGGGAAA ACAGGGCATC CTGGTCTCCC TGGACCTAAG 
GGTGACTGTG GAAAACCAGG TCCTCCTGGC AGCAGTGGCC GGCCAGGTGC AGAGGGTGAG 
CCCGGTGCCA TGGGACCCCA GGGACGACCA GGACCTCCAG GGCATCTTGG GCCACAGGGG 
CCTCCAGGCC AGCCAGGTCC ACCTGGGCTC TCCACCATGG GCCTGAAAGG AGATCGGGGA 
TCCCCTGGAG AAAGGGGCTT GGCCGGCCTC CCAGGCCAGC CTGGCACACC CGGACACCCC 
GGCCCTCCGG GTGAACCTGG TTCGGATGGT GCAGCTGGCA AAGAGGGACC TCCAGGAAAG 
CAGGGACTCT ATGGACCCCC CGGCCCAAAG GGTGACCCAG GACCTGCAGG ACAGAAAGGC 
CAAGCAGGAG AAAAGGGAAG ATCTGGCATG CCTGGGGGCC CTGGCAAGAG TGGCTCCATG 
GGACCGATTG GACCACCGGG TCCTGCGGGA GAGAGAGGCC ACCCTGGGTC CCCAGGGCCT 
GCTGGGAACC CTGGATTGCC TGGTTTGCCA GGCTCTATGG GAGACATGGT GAATTATGAT 
GATATCAAGA GGTTTATCAG ACAAGAGATC ATTAAACTGT TTGATGAGAG GATGGCTTAC 
TACACCTCTA GGATGCAGTT CCCCATGGAG GTGGCAGCAG CTCCAGGCCG GCCAGGGCCT 
CCAGGGAAGG ATGGCGCCCC AGGGCGGCCA GGTGCTCCAG GGTCACCTGG GCTCCCTGGT 
CAGATAGGCC GAGAAGGACG CCAGGGTTTG CCAGGAATGA GAGGATTGCC TGGTACCAAA 
GGTGATAAGG GAGACATTGG TGTTGGCATT GCAGGAGAAA ATGGTCTCCC TGGCCCTCCA 
GGTCCTCAAG GGCCTCCAGG TTATGGCAAG ATGGGTGCAA CCGGGCCAAT GGGCCAGCAA 
GGCATTCCTG GGATCCCTGG CCCCCCGGGG CCCATGGGCC AGCCAGGGAA GGCAGGCCAC 
TGCAACCCCT CAGACTGTTT TGGGGCCATG CCAATGGAGC AGCAGTACCC GCCCATAAAA 
AGCATGAAGG GGCCTTTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239009.1
CDS	355..5178
Translation	MLVSWAPGLWVLGLWATFSHGTNIGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGPPGFPGAVGSPGLPGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238947.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238947.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GCCCCCCAGG TTTTCCTGGT 
GCTGTTGGCT CCCCCGGATT GCCTGGTCTT CAAGGGGAGC GAGGCCCCAC AGGCCTGACT 
GGAGACAAGG GAGAACCGGG TCCCCCAGGA CAGCCTGGCT ACCCGGGTGC CATGGGTCCC 
CCAGGACTGC CTGGCATCAA GGGAGAAAGA GGATATGCTG GCCCTGCGGG AGAGAAGGGG 
GAGTCGGGCC CACCAGGATC TGAAGGCCTT CCAGGTCCCC AAGGTCCAGC GGGTCCCCGA 
GGAGAGCGAG GTCCCCAAGG GAGCTCGGGA GAGAAGGGTG ATCAGGGATT TCAAGGCCAG 
CCAGGTTTCC CTGGCCCACC GGGTCCCCCG GGATTCCCAG GCAAAGCTGG AGCTCCTGGC 
CCACCTGGCC CCCAAGCAGA GAAGGGCAGT GAAGGGATGC GAGGTCCCTC AGGCTTGCCT 
GGTTCCCCTG GACCACCAGG CCCTCCTGGG ATTCAGGGCC CTGCTGGACT GGATGGACTG 
GATGGGAAAG ACGGCAAACC GGGCCTGAGG GGGGACCCAG GTCCCGCTGG CCCTCCTGGA 
CTCATGGGAC CTCCAGGTTT CAAAGGGAAA ACAGGGCATC CTGGTCTCCC TGGACCTAAG 
GGTGACTGTG GAAAACCAGG TCCTCCTGGC AGCAGTGGCC GGCCAGGTGC AGAGGGTGAG 
CCCGGTGCCA TGGGACCCCA GGGACGACCA GGACCTCCAG GGCATCTTGG GCCACAGGGG 
CCTCCAGGCC AGCCAGGTCC ACCTGGGCTC TCCACCATGG GCCTGAAAGG AGATCGGGGA 
TCCCCTGGAG AAAGGGGCTT GGCCGGCCTC CCAGGCCAGC CTGGCACACC CGGACACCCC 
GGCCCTCCGG GTGAACCTGG TTCGGATGGT GCAGCTGGCA AAGAGGGACC TCCAGGAAAG 
CAGGGACTCT ATGGACCCCC CGGCCCAAAG GGTGACCCAG GACCTGCAGG ACAGAAAGGC 
CAAGCAGGAG AAAAGGGAAG ATCTGGCATG CCTGGGGGCC CTGGCAAGAG TGGCTCCATG 
GGACCGATTG GACCACCGGG TCCTGCGGGA GAGAGAGGCC ACCCTGGGTC CCCAGGGCCT 
GCTGGGAACC CTGGATTGCC TGGTTTGCCA GGCTCTATGG GAGACATGGT GAATTATGAT 
GATATCAAGA GGTTTATCAG ACAAGAGATC ATTAAACTGT TTGATGAGAG GATGGCTTAC 
TACACCTCTA GGATGCAGTT CCCCATGGAG GTGGCAGCAG CTCCAGGCCG GCCAGGGCCT 
CCAGGGAAGG ATGGCGCCCC AGGGCGGCCA GGTGCTCCAG GGTCACCTGG GCTCCCTGGT 
CAGATAGGCC GAGAAGGACG CCAGGGTTTG CCAGGAATGA GAGGATTGCC TGGTACCAAA 
GGTGATAAGG GAGACATTGG TGTTGGCATT GCAGGAGAAA ATGGTCTCCC TGGCCCTCCA 
GGTCCTCAAG GGCCTCCAGG TTATGGCAAG ATGGGTGCAA CCGGGCCAAT GGGCCAGCAA 
GGCATTCCTG GGATCCCTGG CCCCCCGGGG CCCATGGGCC AGCCAGGGAA GGCAGGCCAC 
TGCAACCCCT CAGACTGTTT TGGGGCCATG CCAATGGAGC AGCAGTACCC GCCCATAAAA 
AGCATGAAGG GGCCTTTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238948.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239010.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X4, mRNA.

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CloneID	ORa28643
Clone ID Related Accession (Same CDS sequence)	XM_006238948.3
Accession Version	XM_006238948.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238948.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGT 
CTTCAAGGGG AGCGAGGCCC CACAGGCCTG ACTGGAGACA AGGGAGAACC GGGTCCCCCA 
GGACAGCCTG GCTACCCGGG TGCCATGGGT CCCCCAGGAC TGCCTGGCAT CAAGGGAGAA 
AGAGGATATG CTGGCCCTGC GGGAGAGAAG GGGGAGTCGG GCCCACCAGG ATCTGAAGGC 
CTTCCAGGTC CCCAAGGTCC AGCGGGTCCC CGAGGAGAGC GAGGTCCCCA AGGGAGCTCG 
GGAGAGAAGG GTGATCAGGG ATTTCAAGGC CAGCCAGGTT TCCCTGGCCC ACCGGGTCCC 
CCGGGATTCC CAGGCAAAGC TGGAGCTCCT GGCCCACCTG GCCCCCAAGC AGAGAAGGGC 
AGTGAAGGGA TGCGAGGTCC CTCAGGCTTG CCTGGTTCCC CTGGACCACC AGGCCCTCCT 
GGGATTCAGG GCCCTGCTGG ACTGGATGGA CTGGATGGGA AAGACGGCAA ACCGGGCCTG 
AGGGGGGACC CAGGTCCCGC TGGCCCTCCT GGACTCATGG GACCTCCAGG TTTCAAAGGG 
AAAACAGGGC ATCCTGGTCT CCCTGGACCT AAGGGTGACT GTGGAAAACC AGGTCCTCCT 
GGCAGCAGTG GCCGGCCAGG TGCAGAGGGT GAGCCCGGTG CCATGGGACC CCAGGGACGA 
CCAGGACCTC CAGGGCATCT TGGGCCACAG GGGCCTCCAG GCCAGCCAGG TCCACCTGGG 
CTCTCCACCA TGGGCCTGAA AGGAGATCGG GGATCCCCTG GAGAAAGGGG CTTGGCCGGC 
CTCCCAGGCC AGCCTGGCAC ACCCGGACAC CCCGGCCCTC CGGGTGAACC TGGTTCGGAT 
GGTGCAGCTG GCAAAGAGGG ACCTCCAGGA AAGCAGGGAC TCTATGGACC CCCCGGCCCA 
AAGGGTGACC CAGGACCTGC AGGACAGAAA GGCCAAGCAG GAGAAAAGGG AAGATCTGGC 
ATGCCTGGGG GCCCTGGCAA GAGTGGCTCC ATGGGACCGA TTGGACCACC GGGTCCTGCG 
GGAGAGAGAG GCCACCCTGG GTCCCCAGGG CCTGCTGGGA ACCCTGGATT GCCTGGTTTG 
CCAGGCTCTA TGGGAGACAT GGTGAATTAT GATGATATCA AGAGGTTTAT CAGACAAGAG 
ATCATTAAAC TGTTTGATGA GAGGATGGCT TACTACACCT CTAGGATGCA GTTCCCCATG 
GAGGTGGCAG CAGCTCCAGG CCGGCCAGGG CCTCCAGGGA AGGATGGCGC CCCAGGGCGG 
CCAGGTGCTC CAGGGTCACC TGGGCTCCCT GGTCAGATAG GCCGAGAAGG ACGCCAGGGT 
TTGCCAGGAA TGAGAGGATT GCCTGGTACC AAAGGTGATA AGGGAGACAT TGGTGTTGGC 
ATTGCAGGAG AAAATGGTCT CCCTGGCCCT CCAGGTCCTC AAGGGCCTCC AGGTTATGGC 
AAGATGGGTG CAACCGGGCC AATGGGCCAG CAAGGCATTC CTGGGATCCC TGGCCCCCCG 
GGGCCCATGG GCCAGCCAGG GAAGGCAGGC CACTGCAACC CCTCAGACTG TTTTGGGGCC 
ATGCCAATGG AGCAGCAGTA CCCGCCCATA AAAAGCATGA AGGGGCCTTT TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239010.1
CDS	356..5152
Translation	MLVSWAPGLWVLGLWATFSHGTNIGANSVPGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238948.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238948.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
GCTCCGGGAC TGTGGATGGG CAGCTCCTCG CAGCCGGGCC CGCAGGGTCC CCCAGGTGTC 
CCCGGACCAC CAGGTCCCCC CGGAATGCCT GGATTGCAGG GAGTGCCTGG ACACAACGGT 
TTGCCAGGAC AGCCTGGGCT CACTGCCGAG CTGGGATCCT TACCCATCGA GCAGCATCTC 
CTCAAGAGCA TCTGTGGTGA CTGTGCCCAG GGCCAGGCAG CCCATTCAGC CTCCCTTCTG 
GTGAAAGGAG AAAAGGGGGA CCAGGGTGTC CCTGGTGTGC CGGGTTTCAA CGACTGTGCC 
AGGTGCTTCA TAGAGAGGGA GCGCCCGAGA GCTGAGGAGG CCAGGGGAGA CAACAGTGAG 
GGAGAACCTG GCTGTCCTGG GAGCCCTGGC CTGCCTGGTC CTCCAGGGAT GCCTGGCCAA 
AGAGGAGAAG AGGGTCCACC TGGCATGAGG GGCTCTCCTG GTCTTCCAGG CCCTGTTGGT 
CTTCAAGGGG AGCGAGGCCC CACAGGCCTG ACTGGAGACA AGGGAGAACC GGGTCCCCCA 
GGACAGCCTG GCTACCCGGG TGCCATGGGT CCCCCAGGAC TGCCTGGCAT CAAGGGAGAA 
AGAGGATATG CTGGCCCTGC GGGAGAGAAG GGGGAGTCGG GCCCACCAGG ATCTGAAGGC 
CTTCCAGGTC CCCAAGGTCC AGCGGGTCCC CGAGGAGAGC GAGGTCCCCA AGGGAGCTCG 
GGAGAGAAGG GTGATCAGGG ATTTCAAGGC CAGCCAGGTT TCCCTGGCCC ACCGGGTCCC 
CCGGGATTCC CAGGCAAAGC TGGAGCTCCT GGCCCACCTG GCCCCCAAGC AGAGAAGGGC 
AGTGAAGGGA TGCGAGGTCC CTCAGGCTTG CCTGGTTCCC CTGGACCACC AGGCCCTCCT 
GGGATTCAGG GCCCTGCTGG ACTGGATGGA CTGGATGGGA AAGACGGCAA ACCGGGCCTG 
AGGGGGGACC CAGGTCCCGC TGGCCCTCCT GGACTCATGG GACCTCCAGG TTTCAAAGGG 
AAAACAGGGC ATCCTGGTCT CCCTGGACCT AAGGGTGACT GTGGAAAACC AGGTCCTCCT 
GGCAGCAGTG GCCGGCCAGG TGCAGAGGGT GAGCCCGGTG CCATGGGACC CCAGGGACGA 
CCAGGACCTC CAGGGCATCT TGGGCCACAG GGGCCTCCAG GCCAGCCAGG TCCACCTGGG 
CTCTCCACCA TGGGCCTGAA AGGAGATCGG GGATCCCCTG GAGAAAGGGG CTTGGCCGGC 
CTCCCAGGCC AGCCTGGCAC ACCCGGACAC CCCGGCCCTC CGGGTGAACC TGGTTCGGAT 
GGTGCAGCTG GCAAAGAGGG ACCTCCAGGA AAGCAGGGAC TCTATGGACC CCCCGGCCCA 
AAGGGTGACC CAGGACCTGC AGGACAGAAA GGCCAAGCAG GAGAAAAGGG AAGATCTGGC 
ATGCCTGGGG GCCCTGGCAA GAGTGGCTCC ATGGGACCGA TTGGACCACC GGGTCCTGCG 
GGAGAGAGAG GCCACCCTGG GTCCCCAGGG CCTGCTGGGA ACCCTGGATT GCCTGGTTTG 
CCAGGCTCTA TGGGAGACAT GGTGAATTAT GATGATATCA AGAGGTTTAT CAGACAAGAG 
ATCATTAAAC TGTTTGATGA GAGGATGGCT TACTACACCT CTAGGATGCA GTTCCCCATG 
GAGGTGGCAG CAGCTCCAGG CCGGCCAGGG CCTCCAGGGA AGGATGGCGC CCCAGGGCGG 
CCAGGTGCTC CAGGGTCACC TGGGCTCCCT GGTCAGATAG GCCGAGAAGG ACGCCAGGGT 
TTGCCAGGAA TGAGAGGATT GCCTGGTACC AAAGGTGATA AGGGAGACAT TGGTGTTGGC 
ATTGCAGGAG AAAATGGTCT CCCTGGCCCT CCAGGTCCTC AAGGGCCTCC AGGTTATGGC 
AAGATGGGTG CAACCGGGCC AATGGGCCAG CAAGGCATTC CTGGGATCCC TGGCCCCCCG 
GGGCCCATGG GCCAGCCAGG GAAGGCAGGC CACTGCAACC CCTCAGACTG TTTTGGGGCC 
ATGCCAATGG AGCAGCAGTA CCCGCCCATA AAAAGCATGA AGGGGCCTTT TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238950.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239012.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X5, mRNA.

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CloneID	ORa28645
Clone ID Related Accession (Same CDS sequence)	XM_006238950.3
Accession Version	XM_006238950.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238950.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
CCCCCAGGTG TCCCCGGACC ACCAGGTCCC CCCGGAATGC CTGGATTGCA GGGAGTGCCT 
GGACACAACG GTTTGCCAGG ACAGCCTGGG CTCACTGCCG AGCTGGGATC CTTACCCATC 
GAGCAGCATC TCCTCAAGAG CATCTGTGGT GACTGTGCCC AGGGCCAGGC AGCCCATTCA 
GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG GACCAGGGTG TCCCTGGTGT GCCGGGTTTC 
AACGACTGTG CCAGGTGCTT CATAGAGAGG GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA 
GACAACAGTG AGGGAGAACC TGGCTGTCCT GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG 
ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA CCTGGCATGA GGGGCTCTCC TGGTCTTCCA 
GGCCCTGTTG GCCCCCCAGG TTTTCCTGGT GCTGTTGGCT CCCCCGGATT GCCTGGTCTT 
CAAGGGGAGC GAGGCCCCAC AGGCCTGACT GGAGACAAGG GAGAACCGGG TCCCCCAGGA 
CAGCCTGGCT ACCCGGGTGC CATGGGTCCC CCAGGACTGC CTGGCATCAA GGGAGAAAGA 
GGATATGCTG GCCCTGCGGG AGAGAAGGGG GAGTCGGGCC CACCAGGATC TGAAGGCCTT 
CCAGGTCCCC AAGGTCCAGC GGGTCCCCGA GGAGAGCGAG GTCCCCAAGG GAGCTCGGGA 
GAGAAGGGTG ATCAGGGATT TCAAGGCCAG CCAGGTTTCC CTGGCCCACC GGGTCCCCCG 
GGATTCCCAG GCAAAGCTGG AGCTCCTGGC CCACCTGGCC CCCAAGCAGA GAAGGGCAGT 
GAAGGGATGC GAGGTCCCTC AGGCTTGCCT GGTTCCCCTG GACCACCAGG CCCTCCTGGG 
ATTCAGGGCC CTGCTGGACT GGATGGACTG GATGGGAAAG ACGGCAAACC GGGCCTGAGG 
GGGGACCCAG GTCCCGCTGG CCCTCCTGGA CTCATGGGAC CTCCAGGTTT CAAAGGGAAA 
ACAGGGCATC CTGGTCTCCC TGGACCTAAG GGTGACTGTG GAAAACCAGG TCCTCCTGGC 
AGCAGTGGCC GGCCAGGTGC AGAGGGTGAG CCCGGTGCCA TGGGACCCCA GGGACGACCA 
GGACCTCCAG GGCATCTTGG GCCACAGGGG CCTCCAGGCC AGCCAGGTCC ACCTGGGCTC 
TCCACCATGG GCCTGAAAGG AGATCGGGGA TCCCCTGGAG AAAGGGGCTT GGCCGGCCTC 
CCAGGCCAGC CTGGCACACC CGGACACCCC GGCCCTCCGG GTGAACCTGG TTCGGATGGT 
GCAGCTGGCA AAGAGGGACC TCCAGGAAAG CAGGGACTCT ATGGACCCCC CGGCCCAAAG 
GGTGACCCAG GACCTGCAGG ACAGAAAGGC CAAGCAGGAG AAAAGGGAAG ATCTGGCATG 
CCTGGGGGCC CTGGCAAGAG TGGCTCCATG GGACCGATTG GACCACCGGG TCCTGCGGGA 
GAGAGAGGCC ACCCTGGGTC CCCAGGGCCT GCTGGGAACC CTGGATTGCC TGGTTTGCCA 
GGCTCTATGG GAGACATGGT GAATTATGAT GATATCAAGA GGTTTATCAG ACAAGAGATC 
ATTAAACTGT TTGATGAGAG GATGGCTTAC TACACCTCTA GGATGCAGTT CCCCATGGAG 
GTGGCAGCAG CTCCAGGCCG GCCAGGGCCT CCAGGGAAGG ATGGCGCCCC AGGGCGGCCA 
GGTGCTCCAG GGTCACCTGG GCTCCCTGGT CAGATAGGCC GAGAAGGACG CCAGGGTTTG 
CCAGGAATGA GAGGATTGCC TGGTACCAAA GGTGATAAGG GAGACATTGG TGTTGGCATT 
GCAGGAGAAA ATGGTCTCCC TGGCCCTCCA GGTCCTCAAG GGCCTCCAGG TTATGGCAAG 
ATGGGTGCAA CCGGGCCAAT GGGCCAGCAA GGCATTCCTG GGATCCCTGG CCCCCCGGGG 
CCCATGGGCC AGCCAGGGAA GGCAGGCCAC TGCAACCCCT CAGACTGTTT TGGGGCCATG 
CCAATGGAGC AGCAGTACCC GCCCATAAAA AGCATGAAGG GGCCTTTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239012.1
CDS	356..5149
Translation	MLVSWAPGLWVLGLWATFSHGTNIGANSVPGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGPPGFPGAVGSPGLPGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238950.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238950.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GGGAACCCTG CTCATCCTGC 
AGTTCAGGTG TTGGGGCCCA GCATCTTGGG CCCTCCACTG GGGTCAATGA AGACATGGGC 
TCCCCTGTCC ATGGTTTACC TGGCCTTCCG GGCAAACCTG GAATTCCCGG GCCAAGAGGC 
ATGAAAGGAG AGAAGGGTAG TTTTGGCGAT GCTGGGCCGG CTGGAGTTGC GGGACCACCA 
GGACCAGTGG GACCGGCTGG TATCAAAGGG GCAAAGGGGG AGCCGTGTGA GCCCTGTGCA 
TCTTTGTCCA AGCTCCAAGA TGGGGACACT CAAGTGGTTC ACCTGCCCGG CCCAGCTGGA 
GAGAAGGGAG AGCCTGGGCC CCCGGGCTCC GGGCTGCCTG GGAAGCAGGG TAGAGCTGGA 
GAGCGTGGAC TCAAGGGGCA GAAGGGGGAT GCTGGGAATC CAGGAGACCC TGGAACGCCA 
GGCATCACAG GGCAGCCTGG AATGTCTGGA GAGCCTGGGG TTCGGGGTCC TGCAGGACCA 
AAAGGAGAAA AGGGCGATGG ATGCACTGCC TGCCCTAGCC TGCAGGGGGC GTTGACAGAT 
GTATCGGGAC TGCCTGGGAA ACCAGGTCCC AAAGGAGAGC CAGGTCCTGA AGGTGTGGGT 
CGGCCCGGTA AACCAGGCCA GCCTGGTCTA CCAGGAGTTC AAGGGCCTCC AGGACTGAAG 
GGCACTCAGG GAGAGCCTGG ACCTCCAGGC ACCGGTGCTG AGGGACCCCA GGGGGAGCCT 
GGAACCCCAG GTTTGCCTGG CATTCAGGGG CCCCCCGGAC CTCGGGGACC ACCTGGCTCA 
ACCGGCGAAC ACGGTGCCCA GGGACCTCCA GGGCCCAAAG GAGCCACAGG ACCCATGGGA 
CCTCCTGGTG CTAGTATCTC TGGGCCTCCG GGCCAAAAGG GATCACGAGG AGAGAAAGGT 
GAACCGGGAG AGTGTTCCTG TCCTTCTCGA GGAGAGCCCA TCTTCTCCGG CATGCCGGGT 
CCCCCAGGTG TCCCCGGACC ACCAGGTCCC CCCGGAATGC CTGGATTGCA GGGAGTGCCT 
GGACACAACG GTTTGCCAGG ACAGCCTGGG CTCACTGCCG AGCTGGGATC CTTACCCATC 
GAGCAGCATC TCCTCAAGAG CATCTGTGGT GACTGTGCCC AGGGCCAGGC AGCCCATTCA 
GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG GACCAGGGTG TCCCTGGTGT GCCGGGTTTC 
AACGACTGTG CCAGGTGCTT CATAGAGAGG GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA 
GACAACAGTG AGGGAGAACC TGGCTGTCCT GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG 
ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA CCTGGCATGA GGGGCTCTCC TGGTCTTCCA 
GGCCCTGTTG GCCCCCCAGG TTTTCCTGGT GCTGTTGGCT CCCCCGGATT GCCTGGTCTT 
CAAGGGGAGC GAGGCCCCAC AGGCCTGACT GGAGACAAGG GAGAACCGGG TCCCCCAGGA 
CAGCCTGGCT ACCCGGGTGC CATGGGTCCC CCAGGACTGC CTGGCATCAA GGGAGAAAGA 
GGATATGCTG GCCCTGCGGG AGAGAAGGGG GAGTCGGGCC CACCAGGATC TGAAGGCCTT 
CCAGGTCCCC AAGGTCCAGC GGGTCCCCGA GGAGAGCGAG GTCCCCAAGG GAGCTCGGGA 
GAGAAGGGTG ATCAGGGATT TCAAGGCCAG CCAGGTTTCC CTGGCCCACC GGGTCCCCCG 
GGATTCCCAG GCAAAGCTGG AGCTCCTGGC CCACCTGGCC CCCAAGCAGA GAAGGGCAGT 
GAAGGGATGC GAGGTCCCTC AGGCTTGCCT GGTTCCCCTG GACCACCAGG CCCTCCTGGG 
ATTCAGGGCC CTGCTGGACT GGATGGACTG GATGGGAAAG ACGGCAAACC GGGCCTGAGG 
GGGGACCCAG GTCCCGCTGG CCCTCCTGGA CTCATGGGAC CTCCAGGTTT CAAAGGGAAA 
ACAGGGCATC CTGGTCTCCC TGGACCTAAG GGTGACTGTG GAAAACCAGG TCCTCCTGGC 
AGCAGTGGCC GGCCAGGTGC AGAGGGTGAG CCCGGTGCCA TGGGACCCCA GGGACGACCA 
GGACCTCCAG GGCATCTTGG GCCACAGGGG CCTCCAGGCC AGCCAGGTCC ACCTGGGCTC 
TCCACCATGG GCCTGAAAGG AGATCGGGGA TCCCCTGGAG AAAGGGGCTT GGCCGGCCTC 
CCAGGCCAGC CTGGCACACC CGGACACCCC GGCCCTCCGG GTGAACCTGG TTCGGATGGT 
GCAGCTGGCA AAGAGGGACC TCCAGGAAAG CAGGGACTCT ATGGACCCCC CGGCCCAAAG 
GGTGACCCAG GACCTGCAGG ACAGAAAGGC CAAGCAGGAG AAAAGGGAAG ATCTGGCATG 
CCTGGGGGCC CTGGCAAGAG TGGCTCCATG GGACCGATTG GACCACCGGG TCCTGCGGGA 
GAGAGAGGCC ACCCTGGGTC CCCAGGGCCT GCTGGGAACC CTGGATTGCC TGGTTTGCCA 
GGCTCTATGG GAGACATGGT GAATTATGAT GATATCAAGA GGTTTATCAG ACAAGAGATC 
ATTAAACTGT TTGATGAGAG GATGGCTTAC TACACCTCTA GGATGCAGTT CCCCATGGAG 
GTGGCAGCAG CTCCAGGCCG GCCAGGGCCT CCAGGGAAGG ATGGCGCCCC AGGGCGGCCA 
GGTGCTCCAG GGTCACCTGG GCTCCCTGGT CAGATAGGCC GAGAAGGACG CCAGGGTTTG 
CCAGGAATGA GAGGATTGCC TGGTACCAAA GGTGATAAGG GAGACATTGG TGTTGGCATT 
GCAGGAGAAA ATGGTCTCCC TGGCCCTCCA GGTCCTCAAG GGCCTCCAGG TTATGGCAAG 
ATGGGTGCAA CCGGGCCAAT GGGCCAGCAA GGCATTCCTG GGATCCCTGG CCCCCCGGGG 
CCCATGGGCC AGCCAGGGAA GGCAGGCCAC TGCAACCCCT CAGACTGTTT TGGGGCCATG 
CCAATGGAGC AGCAGTACCC GCCCATAAAA AGCATGAAGG GGCCTTTTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238951.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239013.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X6, mRNA.

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CloneID	ORa28646
Clone ID Related Accession (Same CDS sequence)	XM_006238951.3 , NM_001302967.1
Accession Version	XM_006238951.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GTCTTCAAGG GGAGCGAGGC 
CCCACAGGCC TGACTGGAGA CAAGGGAGAA CCGGGTCCCC CAGGACAGCC TGGCTACCCG 
GGTGCCATGG GTCCCCCAGG ACTGCCTGGC ATCAAGGGAG AAAGAGGATA TGCTGGCCCT 
GCGGGAGAGA AGGGGGAGTC GGGCCCACCA GGATCTGAAG GCCTTCCAGG TCCCCAAGGT 
CCAGCGGGTC CCCGAGGAGA GCGAGGTCCC CAAGGGAGCT CGGGAGAGAA GGGTGATCAG 
GGATTTCAAG GCCAGCCAGG TTTCCCTGGC CCACCGGGTC CCCCGGGATT CCCAGGCAAA 
GCTGGAGCTC CTGGCCCACC TGGCCCCCAA GCAGAGAAGG GCAGTGAAGG GATGCGAGGT 
CCCTCAGGCT TGCCTGGTTC CCCTGGACCA CCAGGCCCTC CTGGGATTCA GGGCCCTGCT 
GGACTGGATG GACTGGATGG GAAAGACGGC AAACCGGGCC TGAGGGGGGA CCCAGGTCCC 
GCTGGCCCTC CTGGACTCAT GGGACCTCCA GGTTTCAAAG GGAAAACAGG GCATCCTGGT 
CTCCCTGGAC CTAAGGGTGA CTGTGGAAAA CCAGGTCCTC CTGGCAGCAG TGGCCGGCCA 
GGTGCAGAGG GTGAGCCCGG TGCCATGGGA CCCCAGGGAC GACCAGGACC TCCAGGGCAT 
CTTGGGCCAC AGGGGCCTCC AGGCCAGCCA GGTCCACCTG GGCTCTCCAC CATGGGCCTG 
AAAGGAGATC GGGGATCCCC TGGAGAAAGG GGCTTGGCCG GCCTCCCAGG CCAGCCTGGC 
ACACCCGGAC ACCCCGGCCC TCCGGGTGAA CCTGGTTCGG ATGGTGCAGC TGGCAAAGAG 
GGACCTCCAG GAAAGCAGGG ACTCTATGGA CCCCCCGGCC CAAAGGGTGA CCCAGGACCT 
GCAGGACAGA AAGGCCAAGC AGGAGAAAAG GGAAGATCTG GCATGCCTGG GGGCCCTGGC 
AAGAGTGGCT CCATGGGACC GATTGGACCA CCGGGTCCTG CGGGAGAGAG AGGCCACCCT 
GGGTCCCCAG GGCCTGCTGG GAACCCTGGA TTGCCTGGTT TGCCAGGCTC TATGGGAGAC 
ATGGTGAATT ATGATGATAT CAAGAGGTTT ATCAGACAAG AGATCATTAA ACTGTTTGAT 
GAGAGGATGG CTTACTACAC CTCTAGGATG CAGTTCCCCA TGGAGGTGGC AGCAGCTCCA 
GGCCGGCCAG GGCCTCCAGG GAAGGATGGC GCCCCAGGGC GGCCAGGTGC TCCAGGGTCA 
CCTGGGCTCC CTGGTCAGAT AGGCCGAGAA GGACGCCAGG GTTTGCCAGG AATGAGAGGA 
TTGCCTGGTA CCAAAGGTGA TAAGGGAGAC ATTGGTGTTG GCATTGCAGG AGAAAATGGT 
CTCCCTGGCC CTCCAGGTCC TCAAGGGCCT CCAGGTTATG GCAAGATGGG TGCAACCGGG 
CCAATGGGCC AGCAAGGCAT TCCTGGGATC CCTGGCCCCC CGGGGCCCAT GGGCCAGCCA 
GGGAAGGCAG GCCACTGCAA CCCCTCAGAC TGTTTTGGGG CCATGCCAAT GGAGCAGCAG 
TACCCGCCCA TAAAAAGCAT GAAGGGGCCT TTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239013.1
CDS	355..5133
Translation	MLVSWAPGLWVLGLWATFSHGTNIGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGEPCSSCSSGVGAQHLGPSTGVNEDMGSPVHGLPGLPGKPGIPGPRGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238951.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238951.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGTGAGCG CTGCCCAACT TCACAGGAAG AAGGACTCAA GCTGGAACAC 
AGCAGTGACC CGTCAACCAA CATGACTGGC TTCAACCTAA TCCGCAGGCT GAACCTTATG 
AAGACATCCG CCATCAAAAA GATCCGCAAC CCCAAGGGGC CTCTCATCTT GCGACTGGGG 
GCTGCCCCAC TGACCCAAGC AACTCGACGA GTGTTCCCTC GGGGCCTCCC CGAGGAGTTT 
GCCCTGGTCC TGACGGTCCT TCTGAAGAAA CACACCTTCC GGAATACATG GTACTTATTC 
CAAGTGACTG ACGCAAATGG GTACCCACAG GTCTCCTTGG AAGTCAACAG CCAGGAGCGG 
ACTCTAGAGC TCCGGGCACA GGGCCAAGAT GGTGACTTTG TGTCCTGCAT CTTCCCAGTG 
CCTCAGCTCT TCGATCTGCG TTGGCACAAG CTGATGCTGA GCGTGGCAAG CCGTGTGGCC 
TCTGTGCATG TGGACTGCGT CTCGGCTTCC TCCCAGCCTC TGGGGCCCCG GCAGCCCATC 
CGGCCTGGGG GACATGTGTT TCTGGGCTTG GACGCTGAGC AGGGCAAGCC GGTTTCATTT 
GACCTTCAGC AGGCGCACAT CTACTGTGAC CCGGAGCTGG TGCTGGAGGA AGGCTGCTGT 
GAGATCTTAC CGGGAGGGTG TCCCCCAGAG ACCTCCAAGT CACGCCGGGA CACCCAGAGT 
AATGAGCTCA TTGAGATCAA TCCTCAGACC GAGGGCAAGG TCTACACCCG CTGCTTCTGC 
TTGGAAGAAC CTCATAACAG CAAGGTGGAC GCACAGCTGA TGGGAAGAAG CAGCCAGAAG 
GCAGAAAGGG GGACAAAGGC CCACCAGGAG ACTGGAGCCA GCGAGTGTCC GCCCTGTGCC 
CGCAGCGCCC GGGAGAGCAA TGTCACACTT GGTCCCCCTG GCCTCAAGGG AGGGAAAGGT 
GACCAAGGCC TGACTGGACC ATCAGGCCCC AAGGGAGAGA AGGGAGCACG GGGCAATGAC 
TGTGTTCGAA TCTCCCCAGA TGGCCCACTT CAGTGTGCAG AAGGTCCGAA AGGAGAGAAG 
GGAGAATCAG GAGACTTGGG ACCCTCAGGA CTCCCGGGCC CAACAGGCCA GAAGGGCCAG 
AAAGGGGAGA AGGGCGATGG AGGCCTCAAA GGACTGCCGG GAAAGCCGGG GCGAGATGGT 
CGGCCAGGAG AGATCTGTGT CATTGGGCCC AAAGGGCAGA AAGGAGACCC TGGCTTTGTT 
GGGCCTGAGG GATTGGCAGG AGAGCCTGGG CCCCCTGGCC TACCCGGACC ACCTGGGATA 
GGACTGCCTG GGACTCCTGG AGATCCAGGT GGCCCACCAG GCCCTAAAGG AGCAAAGGGC 
AGCTCTGGAA TACCAGGAAA GGAAGGCCCA GGAGGGAAGC CGGGGAAGCC AGGAGTACCC 
GGGACTAAGG GAGAAAAGGG TGACCCCTGT GAAGTGTGTC CAACGCTGCC CGAAGGGTTC 
CAGAACTTTG TGGGGCTCCC TGGAAAGCCA GGGCCTAAAG GAGAACCAGG TGATCCTGCT 
CCAGCCAGGG AGGGCCTAGG AACTGTTGGA CTGAAAGGTG ACCGGGGAGA TCCAGGCATC 
CAAGGAATGA AAGGAGAGAA GGGGGAACCC TGCTCATCCT GCAGTTCAGG TGTTGGGGCC 
CAGCATCTTG GGCCCTCCAC TGGGGTCAAT GAAGACATGG GCTCCCCTGT CCATGGTTTA 
CCTGGCCTTC CGGGCAAACC TGGAATTCCC GGGCCAAGAG GCATGAAAGG AGAGAAGGGT 
AGTTTTGGCG ATGCTGGGCC GGCTGGAGTT GCGGGACCAC CAGGACCAGT GGGACCGGCT 
GGTATCAAAG GGGCAAAGGG GGAGCCGTGT GAGCCCTGTG CATCTTTGTC CAAGCTCCAA 
GATGGGGACA CTCAAGTGGT TCACCTGCCC GGCCCAGCTG GAGAGAAGGG AGAGCCTGGG 
CCCCCGGGCT CCGGGCTGCC TGGGAAGCAG GGTAGAGCTG GAGAGCGTGG ACTCAAGGGG 
CAGAAGGGGG ATGCTGGGAA TCCAGGAGAC CCTGGAACGC CAGGCATCAC AGGGCAGCCT 
GGAATGTCTG GAGAGCCTGG GGTTCGGGGT CCTGCAGGAC CAAAAGGAGA AAAGGGCGAT 
GGATGCACTG CCTGCCCTAG CCTGCAGGGG GCGTTGACAG ATGTATCGGG ACTGCCTGGG 
AAACCAGGTC CCAAAGGAGA GCCAGGTCCT GAAGGTGTGG GTCGGCCCGG TAAACCAGGC 
CAGCCTGGTC TACCAGGAGT TCAAGGGCCT CCAGGACTGA AGGGCACTCA GGGAGAGCCT 
GGACCTCCAG GCACCGGTGC TGAGGGACCC CAGGGGGAGC CTGGAACCCC AGGTTTGCCT 
GGCATTCAGG GGCCCCCCGG ACCTCGGGGA CCACCTGGCT CAACCGGCGA ACACGGTGCC 
CAGGGACCTC CAGGGCCCAA AGGAGCCACA GGACCCATGG GACCTCCTGG TGCTAGTATC 
TCTGGGCCTC CGGGCCAAAA GGGATCACGA GGAGAGAAAG GTGAACCGGG AGAGTGTTCC 
TGTCCTTCTC GAGGAGAGCC CATCTTCTCC GGCATGCCGG GTGCTCCGGG ACTGTGGATG 
GGCAGCTCCT CGCAGCCGGG CCCGCAGGGT CCCCCAGGTG TCCCCGGACC ACCAGGTCCC 
CCCGGAATGC CTGGATTGCA GGGAGTGCCT GGACACAACG GTTTGCCAGG ACAGCCTGGG 
CTCACTGCCG AGCTGGGATC CTTACCCATC GAGCAGCATC TCCTCAAGAG CATCTGTGGT 
GACTGTGCCC AGGGCCAGGC AGCCCATTCA GCCTCCCTTC TGGTGAAAGG AGAAAAGGGG 
GACCAGGGTG TCCCTGGTGT GCCGGGTTTC AACGACTGTG CCAGGTGCTT CATAGAGAGG 
GAGCGCCCGA GAGCTGAGGA GGCCAGGGGA GACAACAGTG AGGGAGAACC TGGCTGTCCT 
GGGAGCCCTG GCCTGCCTGG TCCTCCAGGG ATGCCTGGCC AAAGAGGAGA AGAGGGTCCA 
CCTGGCATGA GGGGCTCTCC TGGTCTTCCA GGCCCTGTTG GTCTTCAAGG GGAGCGAGGC 
CCCACAGGCC TGACTGGAGA CAAGGGAGAA CCGGGTCCCC CAGGACAGCC TGGCTACCCG 
GGTGCCATGG GTCCCCCAGG ACTGCCTGGC ATCAAGGGAG AAAGAGGATA TGCTGGCCCT 
GCGGGAGAGA AGGGGGAGTC GGGCCCACCA GGATCTGAAG GCCTTCCAGG TCCCCAAGGT 
CCAGCGGGTC CCCGAGGAGA GCGAGGTCCC CAAGGGAGCT CGGGAGAGAA GGGTGATCAG 
GGATTTCAAG GCCAGCCAGG TTTCCCTGGC CCACCGGGTC CCCCGGGATT CCCAGGCAAA 
GCTGGAGCTC CTGGCCCACC TGGCCCCCAA GCAGAGAAGG GCAGTGAAGG GATGCGAGGT 
CCCTCAGGCT TGCCTGGTTC CCCTGGACCA CCAGGCCCTC CTGGGATTCA GGGCCCTGCT 
GGACTGGATG GACTGGATGG GAAAGACGGC AAACCGGGCC TGAGGGGGGA CCCAGGTCCC 
GCTGGCCCTC CTGGACTCAT GGGACCTCCA GGTTTCAAAG GGAAAACAGG GCATCCTGGT 
CTCCCTGGAC CTAAGGGTGA CTGTGGAAAA CCAGGTCCTC CTGGCAGCAG TGGCCGGCCA 
GGTGCAGAGG GTGAGCCCGG TGCCATGGGA CCCCAGGGAC GACCAGGACC TCCAGGGCAT 
CTTGGGCCAC AGGGGCCTCC AGGCCAGCCA GGTCCACCTG GGCTCTCCAC CATGGGCCTG 
AAAGGAGATC GGGGATCCCC TGGAGAAAGG GGCTTGGCCG GCCTCCCAGG CCAGCCTGGC 
ACACCCGGAC ACCCCGGCCC TCCGGGTGAA CCTGGTTCGG ATGGTGCAGC TGGCAAAGAG 
GGACCTCCAG GAAAGCAGGG ACTCTATGGA CCCCCCGGCC CAAAGGGTGA CCCAGGACCT 
GCAGGACAGA AAGGCCAAGC AGGAGAAAAG GGAAGATCTG GCATGCCTGG GGGCCCTGGC 
AAGAGTGGCT CCATGGGACC GATTGGACCA CCGGGTCCTG CGGGAGAGAG AGGCCACCCT 
GGGTCCCCAG GGCCTGCTGG GAACCCTGGA TTGCCTGGTT TGCCAGGCTC TATGGGAGAC 
ATGGTGAATT ATGATGATAT CAAGAGGTTT ATCAGACAAG AGATCATTAA ACTGTTTGAT 
GAGAGGATGG CTTACTACAC CTCTAGGATG CAGTTCCCCA TGGAGGTGGC AGCAGCTCCA 
GGCCGGCCAG GGCCTCCAGG GAAGGATGGC GCCCCAGGGC GGCCAGGTGC TCCAGGGTCA 
CCTGGGCTCC CTGGTCAGAT AGGCCGAGAA GGACGCCAGG GTTTGCCAGG AATGAGAGGA 
TTGCCTGGTA CCAAAGGTGA TAAGGGAGAC ATTGGTGTTG GCATTGCAGG AGAAAATGGT 
CTCCCTGGCC CTCCAGGTCC TCAAGGGCCT CCAGGTTATG GCAAGATGGG TGCAACCGGG 
CCAATGGGCC AGCAAGGCAT TCCTGGGATC CCTGGCCCCC CGGGGCCCAT GGGCCAGCCA 
GGGAAGGCAG GCCACTGCAA CCCCTCAGAC TGTTTTGGGG CCATGCCAAT GGAGCAGCAG 
TACCCGCCCA TAAAAAGCAT GAAGGGGCCT TTTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col16a1 ( XM_006238953.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239015.1 Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa28648
Clone ID Related Accession (Same CDS sequence)	XM_006238953.3
Accession Version	XM_006238953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(XVI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GTAGTTTTGG CGATGCTGGG 
CCGGCTGGAG TTGCGGGACC ACCAGGACCA GTGGGACCGG CTGGTATCAA AGGGGCAAAG 
GGGGAGCCGT GTGAGCCCTG TGCATCTTTG TCCAAGCTCC AAGATGGGGA CACTCAAGTG 
GTTCACCTGC CCGGCCCAGC TGGAGAGAAG GGAGAGCCTG GGCCCCCGGG CTCCGGGCTG 
CCTGGGAAGC AGGGTAGAGC TGGAGAGCGT GGACTCAAGG GGCAGAAGGG GGATGCTGGG 
AATCCAGGAG ACCCTGGAAC GCCAGGCATC ACAGGGCAGC CTGGAATGTC TGGAGAGCCT 
GGGGTTCGGG GTCCTGCAGG ACCAAAAGGA GAAAAGGGCG ATGGATGCAC TGCCTGCCCT 
AGCCTGCAGG GGGCGTTGAC AGATGTATCG GGACTGCCTG GGAAACCAGG TCCCAAAGGA 
GAGCCAGGTC CTGAAGGTGT GGGTCGGCCC GGTAAACCAG GCCAGCCTGG TCTACCAGGA 
GTTCAAGGGC CTCCAGGACT GAAGGGCACT CAGGGAGAGC CTGGACCTCC AGGCACCGGT 
GCTGAGGGAC CCCAGGGGGA GCCTGGAACC CCAGGTTTGC CTGGCATTCA GGGGCCCCCC 
GGACCTCGGG GACCACCTGG CTCAACCGGC GAACACGGTG CCCAGGGACC TCCAGGGCCC 
AAAGGAGCCA CAGGACCCAT GGGACCTCCT GGTGCTAGTA TCTCTGGGCC TCCGGGCCAA 
AAGGGATCAC GAGGAGAGAA AGGTGAACCG GGAGAGTGTT CCTGTCCTTC TCGAGGAGAG 
CCCATCTTCT CCGGCATGCC GGGTGCTCCG GGACTGTGGA TGGGCAGCTC CTCGCAGCCG 
GGCCCGCAGG GTCCCCCAGG TGTCCCCGGA CCACCAGGTC CCCCCGGAAT GCCTGGATTG 
CAGGGAGTGC CTGGACACAA CGGTTTGCCA GGACAGCCTG GGCTCACTGC CGAGCTGGGA 
TCCTTACCCA TCGAGCAGCA TCTCCTCAAG AGCATCTGTG GTGACTGTGC CCAGGGCCAG 
GCAGCCCATT CAGCCTCCCT TCTGGTGAAA GGAGAAAAGG GGGACCAGGG TGTCCCTGGT 
GTGCCGGGTT TCAACGACTG TGCCAGGTGC TTCATAGAGA GGGAGCGCCC GAGAGCTGAG 
GAGGCCAGGG GAGACAACAG TGAGGGAGAA CCTGGCTGTC CTGGGAGCCC TGGCCTGCCT 
GGTCCTCCAG GGATGCCTGG CCAAAGAGGA GAAGAGGGTC CACCTGGCAT GAGGGGCTCT 
CCTGGTCTTC CAGGCCCTGT TGGCCCCCCA GGTTTTCCTG GTGCTGTTGG CTCCCCCGGA 
TTGCCTGGTC TTCAAGGGGA GCGAGGCCCC ACAGGCCTGA CTGGAGACAA GGGAGAACCG 
GGTCCCCCAG GACAGCCTGG CTACCCGGGT GCCATGGGTC CCCCAGGACT GCCTGGCATC 
AAGGGAGAAA GAGGATATGC TGGCCCTGCG GGAGAGAAGG GGGAGTCGGG CCCACCAGGA 
TCTGAAGGCC TTCCAGGTCC CCAAGGTCCA GCGGGTCCCC GAGGAGAGCG AGGTCCCCAA 
GGGAGCTCGG GAGAGAAGGG TGATCAGGGA TTTCAAGGCC AGCCAGGTTT CCCTGGCCCA 
CCGGGTCCCC CGGGATTCCC AGGCAAAGCT GGAGCTCCTG GCCCACCTGG CCCCCAAGCA 
GAGAAGGGCA GTGAAGGGAT GCGAGGTCCC TCAGGCTTGC CTGGTTCCCC TGGACCACCA 
GGCCCTCCTG GGATTCAGGG CCCTGCTGGA CTGGATGGAC TGGATGGGAA AGACGGCAAA 
CCGGGCCTGA GGGGGGACCC AGGTCCCGCT GGCCCTCCTG GACTCATGGG ACCTCCAGGT 
TTCAAAGGGA AAACAGGGCA TCCTGGTCTC CCTGGACCTA AGGGTGACTG TGGAAAACCA 
GGTCCTCCTG GCAGCAGTGG CCGGCCAGGT GCAGAGGGTG AGCCCGGTGC CATGGGACCC 
CAGGGACGAC CAGGACCTCC AGGGCATCTT GGGCCACAGG GGCCTCCAGG CCAGCCAGGT 
CCACCTGGGC TCTCCACCAT GGGCCTGAAA GGAGATCGGG GATCCCCTGG AGAAAGGGGC 
TTGGCCGGCC TCCCAGGCCA GCCTGGCACA CCCGGACACC CCGGCCCTCC GGGTGAACCT 
GGTTCGGATG GTGCAGCTGG CAAAGAGGGA CCTCCAGGAA AGCAGGGACT CTATGGACCC 
CCCGGCCCAA AGGGTGACCC AGGACCTGCA GGACAGAAAG GCCAAGCAGG AGAAAAGGGA 
AGATCTGGCA TGCCTGGGGG CCCTGGCAAG AGTGGCTCCA TGGGACCGAT TGGACCACCG 
GGTCCTGCGG GAGAGAGAGG CCACCCTGGG TCCCCAGGGC CTGCTGGGAA CCCTGGATTG 
CCTGGTTTGC CAGGCTCTAT GGGAGACATG GTGAATTATG ATGATATCAA GAGGTTTATC 
AGACAAGAGA TCATTAAACT GTTTGATGAG AGGATGGCTT ACTACACCTC TAGGATGCAG 
TTCCCCATGG AGGTGGCAGC AGCTCCAGGC CGGCCAGGGC CTCCAGGGAA GGATGGCGCC 
CCAGGGCGGC CAGGTGCTCC AGGGTCACCT GGGCTCCCTG GTCAGATAGG CCGAGAAGGA 
CGCCAGGGTT TGCCAGGAAT GAGAGGATTG CCTGGTACCA AAGGTGATAA GGGAGACATT 
GGTGTTGGCA TTGCAGGAGA AAATGGTCTC CCTGGCCCTC CAGGTCCTCA AGGGCCTCCA 
GGTTATGGCA AGATGGGTGC AACCGGGCCA ATGGGCCAGC AAGGCATTCC TGGGATCCCT 
GGCCCCCCGG GGCCCATGGG CCAGCCAGGG AAGGCAGGCC ACTGCAACCC CTCAGACTGT 
TTTGGGGCCA TGCCAATGGA GCAGCAGTAC CCGCCCATAA AAAGCATGAA GGGGCCTTTT 
GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239015.1
CDS	359..5044
Translation	MLVSWAPGLWVLGLWATFSHGTNIGANSVPGERCPTSQEEGLKLEHSSDPSTNMTGFNLIRRLNLMKTSAIKKIRNPKGPLILRLGAAPLTQATRRVFPRGLPEEFALVLTVLLKKHTFRNTWYLFQVTDANGYPQVSLEVNSQERTLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVASRVASVHVDCVSASSQPLGPRQPIRPGGHVFLGLDAEQGKPVSFDLQQAHIYCDPELVLEEGCCEILPGGCPPETSKSRRDTQSNELIEINPQTEGKVYTRCFCLEEPHNSKVDAQLMGRSSQKAERGTKAHQETGASECPPCARSARESNVTLGPPGLKGGKGDQGLTGPSGPKGEKGARGNDCVRISPDGPLQCAEGPKGEKGESGDLGPSGLPGPTGQKGQKGEKGDGGLKGLPGKPGRDGRPGEICVIGPKGQKGDPGFVGPEGLAGEPGPPGLPGPPGIGLPGTPGDPGGPPGPKGAKGSSGIPGKEGPGGKPGKPGVPGTKGEKGDPCEVCPTLPEGFQNFVGLPGKPGPKGEPGDPAPAREGLGTVGLKGDRGDPGIQGMKGEKGSFGDAGPAGVAGPPGPVGPAGIKGAKGEPCEPCASLSKLQDGDTQVVHLPGPAGEKGEPGPPGSGLPGKQGRAGERGLKGQKGDAGNPGDPGTPGITGQPGMSGEPGVRGPAGPKGEKGDGCTACPSLQGALTDVSGLPGKPGPKGEPGPEGVGRPGKPGQPGLPGVQGPPGLKGTQGEPGPPGTGAEGPQGEPGTPGLPGIQGPPGPRGPPGSTGEHGAQGPPGPKGATGPMGPPGASISGPPGQKGSRGEKGEPGECSCPSRGEPIFSGMPGAPGLWMGSSSQPGPQGPPGVPGPPGPPGMPGLQGVPGHNGLPGQPGLTAELGSLPIEQHLLKSICGDCAQGQAAHSASLLVKGEKGDQGVPGVPGFNDCARCFIERERPRAEEARGDNSEGEPGCPGSPGLPGPPGMPGQRGEEGPPGMRGSPGLPGPVGPPGFPGAVGSPGLPGLQGERGPTGLTGDKGEPGPPGQPGYPGAMGPPGLPGIKGERGYAGPAGEKGESGPPGSEGLPGPQGPAGPRGERGPQGSSGEKGDQGFQGQPGFPGPPGPPGFPGKAGAPGPPGPQAEKGSEGMRGPSGLPGSPGPPGPPGIQGPAGLDGLDGKDGKPGLRGDPGPAGPPGLMGPPGFKGKTGHPGLPGPKGDCGKPGPPGSSGRPGAEGEPGAMGPQGRPGPPGHLGPQGPPGQPGPPGLSTMGLKGDRGSPGERGLAGLPGQPGTPGHPGPPGEPGSDGAAGKEGPPGKQGLYGPPGPKGDPGPAGQKGQAGEKGRSGMPGGPGKSGSMGPIGPPGPAGERGHPGSPGPAGNPGLPGLPGSMGDMVNYDDIKRFIRQEIIKLFDERMAYYTSRMQFPMEVAAAPGRPGPPGKDGAPGRPGAPGSPGLPGQIGREGRQGLPGMRGLPGTKGDKGDIGVGIAGENGLPGPPGPQGPPGYGKMGATGPMGQQGIPGIPGPPGPMGQPGKAGHCNPSDCFGAMPMEQQYPPIKSMKGPFG

Target ORF information:

RefSeq Version	XM_006238953.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type XVI alpha 1 chain (Col16a1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006238953.3

ORF Insert Sequence:

ATGTTGGTAT CCTGGGCTCC CGGCCTATGG GTGCTTGGGC TCTGGGCCAC CTTCAGCCAT 
GGAACAAATA TAGGGGCCAA CTCTGTCCCA GGTGAGCGCT GCCCAACTTC ACAGGAAGAA 
GGACTCAAGC TGGAACACAG CAGTGACCCG TCAACCAACA TGACTGGCTT CAACCTAATC 
CGCAGGCTGA ACCTTATGAA GACATCCGCC ATCAAAAAGA TCCGCAACCC CAAGGGGCCT 
CTCATCTTGC GACTGGGGGC TGCCCCACTG ACCCAAGCAA CTCGACGAGT GTTCCCTCGG 
GGCCTCCCCG AGGAGTTTGC CCTGGTCCTG ACGGTCCTTC TGAAGAAACA CACCTTCCGG 
AATACATGGT ACTTATTCCA AGTGACTGAC GCAAATGGGT ACCCACAGGT CTCCTTGGAA 
GTCAACAGCC AGGAGCGGAC TCTAGAGCTC CGGGCACAGG GCCAAGATGG TGACTTTGTG 
TCCTGCATCT TCCCAGTGCC TCAGCTCTTC GATCTGCGTT GGCACAAGCT GATGCTGAGC 
GTGGCAAGCC GTGTGGCCTC TGTGCATGTG GACTGCGTCT CGGCTTCCTC CCAGCCTCTG 
GGGCCCCGGC AGCCCATCCG GCCTGGGGGA CATGTGTTTC TGGGCTTGGA CGCTGAGCAG 
GGCAAGCCGG TTTCATTTGA CCTTCAGCAG GCGCACATCT ACTGTGACCC GGAGCTGGTG 
CTGGAGGAAG GCTGCTGTGA GATCTTACCG GGAGGGTGTC CCCCAGAGAC CTCCAAGTCA 
CGCCGGGACA CCCAGAGTAA TGAGCTCATT GAGATCAATC CTCAGACCGA GGGCAAGGTC 
TACACCCGCT GCTTCTGCTT GGAAGAACCT CATAACAGCA AGGTGGACGC ACAGCTGATG 
GGAAGAAGCA GCCAGAAGGC AGAAAGGGGG ACAAAGGCCC ACCAGGAGAC TGGAGCCAGC 
GAGTGTCCGC CCTGTGCCCG CAGCGCCCGG GAGAGCAATG TCACACTTGG TCCCCCTGGC 
CTCAAGGGAG GGAAAGGTGA CCAAGGCCTG ACTGGACCAT CAGGCCCCAA GGGAGAGAAG 
GGAGCACGGG GCAATGACTG TGTTCGAATC TCCCCAGATG GCCCACTTCA GTGTGCAGAA 
GGTCCGAAAG GAGAGAAGGG AGAATCAGGA GACTTGGGAC CCTCAGGACT CCCGGGCCCA 
ACAGGCCAGA AGGGCCAGAA AGGGGAGAAG GGCGATGGAG GCCTCAAAGG ACTGCCGGGA 
AAGCCGGGGC GAGATGGTCG GCCAGGAGAG ATCTGTGTCA TTGGGCCCAA AGGGCAGAAA 
GGAGACCCTG GCTTTGTTGG GCCTGAGGGA TTGGCAGGAG AGCCTGGGCC CCCTGGCCTA 
CCCGGACCAC CTGGGATAGG ACTGCCTGGG ACTCCTGGAG ATCCAGGTGG CCCACCAGGC 
CCTAAAGGAG CAAAGGGCAG CTCTGGAATA CCAGGAAAGG AAGGCCCAGG AGGGAAGCCG 
GGGAAGCCAG GAGTACCCGG GACTAAGGGA GAAAAGGGTG ACCCCTGTGA AGTGTGTCCA 
ACGCTGCCCG AAGGGTTCCA GAACTTTGTG GGGCTCCCTG GAAAGCCAGG GCCTAAAGGA 
GAACCAGGTG ATCCTGCTCC AGCCAGGGAG GGCCTAGGAA CTGTTGGACT GAAAGGTGAC 
CGGGGAGATC CAGGCATCCA AGGAATGAAA GGAGAGAAGG GTAGTTTTGG CGATGCTGGG 
CCGGCTGGAG TTGCGGGACC ACCAGGACCA GTGGGACCGG CTGGTATCAA AGGGGCAAAG 
GGGGAGCCGT GTGAGCCCTG TGCATCTTTG TCCAAGCTCC AAGATGGGGA CACTCAAGTG 
GTTCACCTGC CCGGCCCAGC TGGAGAGAAG GGAGAGCCTG GGCCCCCGGG CTCCGGGCTG 
CCTGGGAAGC AGGGTAGAGC TGGAGAGCGT GGACTCAAGG GGCAGAAGGG GGATGCTGGG 
AATCCAGGAG ACCCTGGAAC GCCAGGCATC ACAGGGCAGC CTGGAATGTC TGGAGAGCCT 
GGGGTTCGGG GTCCTGCAGG ACCAAAAGGA GAAAAGGGCG ATGGATGCAC TGCCTGCCCT 
AGCCTGCAGG GGGCGTTGAC AGATGTATCG GGACTGCCTG GGAAACCAGG TCCCAAAGGA 
GAGCCAGGTC CTGAAGGTGT GGGTCGGCCC GGTAAACCAG GCCAGCCTGG TCTACCAGGA 
GTTCAAGGGC CTCCAGGACT GAAGGGCACT CAGGGAGAGC CTGGACCTCC AGGCACCGGT 
GCTGAGGGAC CCCAGGGGGA GCCTGGAACC CCAGGTTTGC CTGGCATTCA GGGGCCCCCC 
GGACCTCGGG GACCACCTGG CTCAACCGGC GAACACGGTG CCCAGGGACC TCCAGGGCCC 
AAAGGAGCCA CAGGACCCAT GGGACCTCCT GGTGCTAGTA TCTCTGGGCC TCCGGGCCAA 
AAGGGATCAC GAGGAGAGAA AGGTGAACCG GGAGAGTGTT CCTGTCCTTC TCGAGGAGAG 
CCCATCTTCT CCGGCATGCC GGGTGCTCCG GGACTGTGGA TGGGCAGCTC CTCGCAGCCG 
GGCCCGCAGG GTCCCCCAGG TGTCCCCGGA CCACCAGGTC CCCCCGGAAT GCCTGGATTG 
CAGGGAGTGC CTGGACACAA CGGTTTGCCA GGACAGCCTG GGCTCACTGC CGAGCTGGGA 
TCCTTACCCA TCGAGCAGCA TCTCCTCAAG AGCATCTGTG GTGACTGTGC CCAGGGCCAG 
GCAGCCCATT CAGCCTCCCT TCTGGTGAAA GGAGAAAAGG GGGACCAGGG TGTCCCTGGT 
GTGCCGGGTT TCAACGACTG TGCCAGGTGC TTCATAGAGA GGGAGCGCCC GAGAGCTGAG 
GAGGCCAGGG GAGACAACAG TGAGGGAGAA CCTGGCTGTC CTGGGAGCCC TGGCCTGCCT 
GGTCCTCCAG GGATGCCTGG CCAAAGAGGA GAAGAGGGTC CACCTGGCAT GAGGGGCTCT 
CCTGGTCTTC CAGGCCCTGT TGGCCCCCCA GGTTTTCCTG GTGCTGTTGG CTCCCCCGGA 
TTGCCTGGTC TTCAAGGGGA GCGAGGCCCC ACAGGCCTGA CTGGAGACAA GGGAGAACCG 
GGTCCCCCAG GACAGCCTGG CTACCCGGGT GCCATGGGTC CCCCAGGACT GCCTGGCATC 
AAGGGAGAAA GAGGATATGC TGGCCCTGCG GGAGAGAAGG GGGAGTCGGG CCCACCAGGA 
TCTGAAGGCC TTCCAGGTCC CCAAGGTCCA GCGGGTCCCC GAGGAGAGCG AGGTCCCCAA 
GGGAGCTCGG GAGAGAAGGG TGATCAGGGA TTTCAAGGCC AGCCAGGTTT CCCTGGCCCA 
CCGGGTCCCC CGGGATTCCC AGGCAAAGCT GGAGCTCCTG GCCCACCTGG CCCCCAAGCA 
GAGAAGGGCA GTGAAGGGAT GCGAGGTCCC TCAGGCTTGC CTGGTTCCCC TGGACCACCA 
GGCCCTCCTG GGATTCAGGG CCCTGCTGGA CTGGATGGAC TGGATGGGAA AGACGGCAAA 
CCGGGCCTGA GGGGGGACCC AGGTCCCGCT GGCCCTCCTG GACTCATGGG ACCTCCAGGT 
TTCAAAGGGA AAACAGGGCA TCCTGGTCTC CCTGGACCTA AGGGTGACTG TGGAAAACCA 
GGTCCTCCTG GCAGCAGTGG CCGGCCAGGT GCAGAGGGTG AGCCCGGTGC CATGGGACCC 
CAGGGACGAC CAGGACCTCC AGGGCATCTT GGGCCACAGG GGCCTCCAGG CCAGCCAGGT 
CCACCTGGGC TCTCCACCAT GGGCCTGAAA GGAGATCGGG GATCCCCTGG AGAAAGGGGC 
TTGGCCGGCC TCCCAGGCCA GCCTGGCACA CCCGGACACC CCGGCCCTCC GGGTGAACCT 
GGTTCGGATG GTGCAGCTGG CAAAGAGGGA CCTCCAGGAA AGCAGGGACT CTATGGACCC 
CCCGGCCCAA AGGGTGACCC AGGACCTGCA GGACAGAAAG GCCAAGCAGG AGAAAAGGGA 
AGATCTGGCA TGCCTGGGGG CCCTGGCAAG AGTGGCTCCA TGGGACCGAT TGGACCACCG 
GGTCCTGCGG GAGAGAGAGG CCACCCTGGG TCCCCAGGGC CTGCTGGGAA CCCTGGATTG 
CCTGGTTTGC CAGGCTCTAT GGGAGACATG GTGAATTATG ATGATATCAA GAGGTTTATC 
AGACAAGAGA TCATTAAACT GTTTGATGAG AGGATGGCTT ACTACACCTC TAGGATGCAG 
TTCCCCATGG AGGTGGCAGC AGCTCCAGGC CGGCCAGGGC CTCCAGGGAA GGATGGCGCC 
CCAGGGCGGC CAGGTGCTCC AGGGTCACCT GGGCTCCCTG GTCAGATAGG CCGAGAAGGA 
CGCCAGGGTT TGCCAGGAAT GAGAGGATTG CCTGGTACCA AAGGTGATAA GGGAGACATT 
GGTGTTGGCA TTGCAGGAGA AAATGGTCTC CCTGGCCCTC CAGGTCCTCA AGGGCCTCCA 
GGTTATGGCA AGATGGGTGC AACCGGGCCA ATGGGCCAGC AAGGCATTCC TGGGATCCCT 
GGCCCCCCGG GGCCCATGGG CCAGCCAGGG AAGGCAGGCC ACTGCAACCC CTCAGACTGT 
TTTGGGGCCA TGCCAATGGA GCAGCAGTAC CCGCCCATAA AAAGCATGAA GGGGCCTTTT 
GGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Collagen XVI induces expression of MMP9 via modulation of AP-1 transcription factors and facilitates invasion of oral squamous cell carcinoma.
PloS one9(1)e86777(2014)
Bedal KB,Gr?ssel S,Oefner PJ,Reinders J,Reichert TE,Bauer R

Difference in expression of hepatic microRNAs miR-29c, miR-34a, miR-155, and miR-200b is associated with strain-specific susceptibility to dietary nonalcoholic steatohepatitis in mice.
Laboratory investigation; a journal of technical methods and pathology90(10)1437-46(2010 Oct)
Pogribny IP,Starlard-Davenport A,Tryndyak VP,Han T,Ross SA,Rusyn I,Beland FA

Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains.
The Journal of biological chemistry281(35)25745-56(2006 Sep)
Eble JA,Kassner A,Niland S,M?rgelin M,Grifka J,Gr?ssel S

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Col16a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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