Lhx8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lhx8
Entrez Gene ID	365963
Full Name	LIM homeobox 8
Synonyms	Lhx7
General protein information	Preferred Names LIM homeobox 8 Names LIM/homeobox protein Lhx8 LIM homeobox protein 8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q45
Summary	may play a role in development of the cholinergic system [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233528.3	XP_006233590.1	LIM/homeobox protein Lhx8 isoform X1
NM_001012219.1	NP_001012219.1	LIM/homeobox protein Lhx8

Pathways from BioSystems

Homologies

	LHX8	XP_001097664.2
Canis lupus familiaris (dog)	LHX8	XP_005622116.1
Rattus norvegicus (Norway rat)	Lhx8	NP_001012219.1
Bos taurus (cattle)	LHX8	XP_002686347.1
Gallus gallus (chicken)	LHX8	NP_001035556.1
Danio rerio (zebrafish)	lhx8a	NP_001003980.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006540	XP_316578.4
Homo sapiens (human)	LHX8	NP_001001933.1
Xenopus tropicalis (tropical clawed frog)	lhx8	NP_001015899.1
Pan troglodytes (chimpanzee)	LHX8	XP_524738.2
Mus musculus (house mouse)	Lhx8	NP_034843.2
Drosophila melanogaster (fruit fly)	Awh	NP_728906.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest in PC12 cell line.
Li H, Qin J, Jin G, Zou L, Shi J, Han X, Cheng X, Zhang X
In vitro cellular & developmental biology. Animal51(4)329-35(2015 Apr)

LIM homeobox 8 (Lhx8) is a key regulator of the cholinergic neuronal function via a tropomyosin receptor kinase A (TrkA)-mediated positive feedback loop.
Tomioka T, Shimazaki T, Yamauchi T, Oki T, Ohgoh M, Okano H
The Journal of biological chemistry289(2)1000-10(2014 Jan)

LIM-homeobox gene Lhx8 promote the differentiation of hippocampal newborn neurons into cholinergic neurons in vitro.
Zhu P, Li H, Jin G, Tian M, Tan X, Shi J, Zou L, Qin J
In vitro cellular & developmental biology. Animal49(2)103-7(2013 Feb)

Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro.
Shi J, Li H, Jin G, Zhu P, Tian M, Qin J, Tan X, Zhao S, Wang F, Hua Y, Xiao Y
In vitro cellular & developmental biology. Animal48(10)603-9(2012 Dec)

Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression.
Choi Y, Yuan D, Rajkovic A
Biology of reproduction79(6)1176-82(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lhx8 plays role as a suppressor gene of cell proliferation.
Title: Overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest in PC12 cell line.

Data indicate that LIM homeobox 8 (Lhx8) regulates functions of cholinergic neurons by modulating tropomyosin receptor kinase A (TrkA) expression.
Title: LIM homeobox 8 (Lhx8) is a key regulator of the cholinergic neuronal function via a tropomyosin receptor kinase A (TrkA)-mediated positive feedback loop.

Although the potential interaction of Lhx8 with other factors remains unknown, our results suggested that neuronal subtype specification can be directly manipulated in the stage of immature newborn neurons by a specific transcription factor in vitro.
Title: LIM-homeobox gene Lhx8 promote the differentiation of hippocampal newborn neurons into cholinergic neurons in vitro.

Lhx8 was not associated with the MAP2-positive neurons differentiation on adherent conditions, but played a role in the specification of cholinergic neurons derived from hippocampal neural stem/progenitor cells in vitro
Title: Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro.

Combined expression of Lhx7 and Gbx1 plays a role in the development of the cholinergic system of the basal forebrain.
Title: The homeobox genes Lhx7 and Gbx1 are expressed in the basal forebrain cholinergic system.

The following Lhx8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lhx8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12744	NM_001012219.1 Latest version!	Rattus norvegicus LIM homeobox 8 (Lhx8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa25399	XM_006233528.3 Latest version!	Rattus norvegicus LIM homeobox 8 (Lhx8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lhx8 ( NM_001012219.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012219.1 Rattus norvegicus LIM homeobox 8 (Lhx8), mRNA.

Price & Availability↑

CloneID	ORa12744
Clone ID Related Accession (Same CDS sequence)	NM_001012219.1
Accession Version	NM_001012219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	LIM/homeobox protein Lhx8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090011.1. On Feb 11, 2005 this sequence version replaced XM_345303.1. ##Evidence-Data-START## Transcript exon combination :: BC090011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642703, SAMN02642731 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGG AGTGCGGGCG GCCTGCAGCC CTGGCGGCCG GGAGGACCCG CAAAGGCGCT 
GGGGAAGAAG GACTGGTGAA TCCCGAGGGA GCCGGGGACG AGGACTCCTG CTCCTCCTCG 
GCCCCGCTTT CCCCGTCGTC CTCGCCCCAG TCCATGGCCT CGGGCTCCGT GTGCCCACCA 
GGCAAGTGTG TGTGCAGCAG CTGCGGCCTG GAGATTGTGG ACAAATACCT CCTCAAGGTG 
AATGACTTAT GCTGGCATGT CCGCTGTCTC TCCTGCAGTG TCTGCAGAAC GTCCCTGGGA 
AGGCACACAA GCTGTTACAT TAAGGATAAA GACATTTTCT GTAAACTCGA TTACTTCAGA 
CGGTATGGGA CACGCTGCTC CCGCTGTGGA AGGCACATCC ATTCTACTGA CTGGGTCCGC 
AGGGCCAAGG GCAACGTGTA TCACTTGGCT TGCTTTGCCT GCTTTTCTTG CAAGAGGCAA 
CTGTCCACGG GAGAGGAGTT TGCCTTGGTA GAGGAGAAGG TCCTCTGTAG AGTGCACTTT 
GACTGCATGC TGGACAATCT GAAGAGAGAA GTGGAGAACG GTAATGGGAT TAGTGTGGAA 
GGAGCCCTTC TCACAGAACA AGACGTCAAT CATCCAAAGC CAGCCAAAAG AGCTCGGACC 
AGCTTCACAG CCGATCAGCT CCAGGTTATG CAAGCACAGT TTGCTCAGGA CAACAACCCA 
GACGCACAGA CCCTCCAGAA ACTGGCAGAA AGGACAGGCT TAAGCCGGCG AGTCATACAG 
GTGTGGTTTC AGAACTGCAG GGCTCGTCAT AAGAAACATG TCAGTCCCAA CCACTCCTCC 
TCTGCCCCGG TCACAGCAGT CCCCTCATCC AGGCTGTCTC CACCCATGTT GGAAGAAATG 
GCTTATTCCG CCTACGTGCC CCAGGACGGG ACGATGCTGA CGGCGCTGCA TTCATACATG 
GACGCTCACC AACAACTCCT GGACTCCAGC CCTTGCTACC CCATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012219.1
CDS	72..1082
Translation	MSEECGRPAALAAGRTRKGAGEEGLVNPEGAGDEDSCSSSAPLSPSSSPQSMASGSVCPPGKCVCSSCGLEIVDKYLLKVNDLCWHVRCLSCSVCRTSLGRHTSCYIKDKDIFCKLDYFRRYGTRCSRCGRHIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRVHFDCMLDNLKREVENGNGISVEGALLTEQDVNHPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSSAPVTAVPSSRLSPPMLEEMAYSAYVPQDGTMLTALHSYMDAHQQLLDSSPCYPIQ

Target ORF information:

RefSeq Version	NM_001012219.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM homeobox 8 (Lhx8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012219.1

ORF Insert Sequence:

ATGTCGGAGG AGTGCGGGCG GCCTGCAGCC CTGGCGGCCG GGAGGACCCG CAAAGGCGCT 
GGGGAAGAAG GACTGGTGAA TCCCGAGGGA GCCGGGGACG AGGACTCCTG CTCCTCCTCG 
GCCCCGCTTT CCCCGTCGTC CTCGCCCCAG TCCATGGCCT CGGGCTCCGT GTGCCCACCA 
GGCAAGTGTG TGTGCAGCAG CTGCGGCCTG GAGATTGTGG ACAAATACCT CCTCAAGGTG 
AATGACTTAT GCTGGCATGT CCGCTGTCTC TCCTGCAGTG TCTGCAGAAC GTCCCTGGGA 
AGGCACACAA GCTGTTACAT TAAGGATAAA GACATTTTCT GTAAACTCGA TTACTTCAGA 
CGGTATGGGA CACGCTGCTC CCGCTGTGGA AGGCACATCC ATTCTACTGA CTGGGTCCGC 
AGGGCCAAGG GCAACGTGTA TCACTTGGCT TGCTTTGCCT GCTTTTCTTG CAAGAGGCAA 
CTGTCCACGG GAGAGGAGTT TGCCTTGGTA GAGGAGAAGG TCCTCTGTAG AGTGCACTTT 
GACTGCATGC TGGACAATCT GAAGAGAGAA GTGGAGAACG GTAATGGGAT TAGTGTGGAA 
GGAGCCCTTC TCACAGAACA AGACGTCAAT CATCCAAAGC CAGCCAAAAG AGCTCGGACC 
AGCTTCACAG CCGATCAGCT CCAGGTTATG CAAGCACAGT TTGCTCAGGA CAACAACCCA 
GACGCACAGA CCCTCCAGAA ACTGGCAGAA AGGACAGGCT TAAGCCGGCG AGTCATACAG 
GTGTGGTTTC AGAACTGCAG GGCTCGTCAT AAGAAACATG TCAGTCCCAA CCACTCCTCC 
TCTGCCCCGG TCACAGCAGT CCCCTCATCC AGGCTGTCTC CACCCATGTT GGAAGAAATG 
GCTTATTCCG CCTACGTGCC CCAGGACGGG ACGATGCTGA CGGCGCTGCA TTCATACATG 
GACGCTCACC AACAACTCCT GGACTCCAGC CCTTGCTACC CCATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lhx8 ( XM_006233528.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233590.1 Rattus norvegicus LIM homeobox 8 (Lhx8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25399
Clone ID Related Accession (Same CDS sequence)	XM_006233528.3
Accession Version	XM_006233528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LIM/homeobox protein Lhx8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTGGA AGAGCGATCA GATGTTTGTG TGTAAACTGG AGGGAAAGGA CGTGCCGGAG 
CTGGCGGTTC CCCGCGAGAA GTGCCCCGGG CTCATGTCGG AGGAGTGCGG GCGGCCTGCA 
GCCCTGGCGG CCGGGAGGAC CCGCAAAGGC GCTGGGGAAG AAGGACTGGT GAATCCCGAG 
GGAGCCGGGG ACGAGGACTC CTGCTCCTCC TCGGCCCCGC TTTCCCCGTC GTCCTCGCCC 
CAGTCCATGG CCTCGGGCTC CGTGTGCCCA CCAGGCAAGT GTGTGTGCAG CAGCTGCGGC 
CTGGAGATTG TGGACAAATA CCTCCTCAAG GTGAATGACT TATGCTGGCA TGTCCGCTGT 
CTCTCCTGCA GTGTCTGCAG AACGTCCCTG GGAAGGCACA CAAGCTGTTA CATTAAGGAT 
AAAGACATTT TCTGTAAACT CGATTACTTC AGACGGTATG GGACACGCTG CTCCCGCTGT 
GGAAGGCACA TCCATTCTAC TGACTGGGTC CGCAGGGCCA AGGGCAACGT GTATCACTTG 
GCTTGCTTTG CCTGCTTTTC TTGCAAGAGG CAACTGTCCA CGGGAGAGGA GTTTGCCTTG 
GTAGAGGAGA AGGTCCTCTG TAGAGTGCAC TTTGACTGCA TGCTGGACAA TCTGAAGAGA 
GAAGTGGAGA ACGGTAATGG GATTAGTGTG GAAGGAGCCC TTCTCACAGA ACAAGACGTC 
AATCATCCAA AGCCAGCCAA AAGAGCTCGG ACCAGCTTCA CAGCCGATCA GCTCCAGGTT 
ATGCAAGCAC AGTTTGCTCA GGACAACAAC CCAGACGCAC AGACCCTCCA GAAACTGGCA 
GAAAGGACAG GCTTAAGCCG GCGAGTCATA CAGGTGTGGT TTCAGAACTG CAGGGCTCGT 
CATAAGAAAC ATGTCAGTCC CAACCACTCC TCCTCTGCCC CGGTCACAGC AGTCCCCTCA 
TCCAGGCTGT CTCCACCCAT GTTGGAAGAA ATGGCTTATT CCGCCTACGT GCCCCAGGAC 
GGGACGATGC TGACGGCGCT GCATTCATAC ATGGACGCTC ACCAACAACT CCTGGACTCC 
AGCCCTTGCT ACCCCATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233590.1
CDS	245..1348
Translation	MYWKSDQMFVCKLEGKDVPELAVPREKCPGLMSEECGRPAALAAGRTRKGAGEEGLVNPEGAGDEDSCSSSAPLSPSSSPQSMASGSVCPPGKCVCSSCGLEIVDKYLLKVNDLCWHVRCLSCSVCRTSLGRHTSCYIKDKDIFCKLDYFRRYGTRCSRCGRHIHSTDWVRRAKGNVYHLACFACFSCKRQLSTGEEFALVEEKVLCRVHFDCMLDNLKREVENGNGISVEGALLTEQDVNHPKPAKRARTSFTADQLQVMQAQFAQDNNPDAQTLQKLAERTGLSRRVIQVWFQNCRARHKKHVSPNHSSSAPVTAVPSSRLSPPMLEEMAYSAYVPQDGTMLTALHSYMDAHQQLLDSSPCYPIQ

Target ORF information:

RefSeq Version	XM_006233528.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus LIM homeobox 8 (Lhx8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233528.3

ORF Insert Sequence:

ATGTATTGGA AGAGCGATCA GATGTTTGTG TGTAAACTGG AGGGAAAGGA CGTGCCGGAG 
CTGGCGGTTC CCCGCGAGAA GTGCCCCGGG CTCATGTCGG AGGAGTGCGG GCGGCCTGCA 
GCCCTGGCGG CCGGGAGGAC CCGCAAAGGC GCTGGGGAAG AAGGACTGGT GAATCCCGAG 
GGAGCCGGGG ACGAGGACTC CTGCTCCTCC TCGGCCCCGC TTTCCCCGTC GTCCTCGCCC 
CAGTCCATGG CCTCGGGCTC CGTGTGCCCA CCAGGCAAGT GTGTGTGCAG CAGCTGCGGC 
CTGGAGATTG TGGACAAATA CCTCCTCAAG GTGAATGACT TATGCTGGCA TGTCCGCTGT 
CTCTCCTGCA GTGTCTGCAG AACGTCCCTG GGAAGGCACA CAAGCTGTTA CATTAAGGAT 
AAAGACATTT TCTGTAAACT CGATTACTTC AGACGGTATG GGACACGCTG CTCCCGCTGT 
GGAAGGCACA TCCATTCTAC TGACTGGGTC CGCAGGGCCA AGGGCAACGT GTATCACTTG 
GCTTGCTTTG CCTGCTTTTC TTGCAAGAGG CAACTGTCCA CGGGAGAGGA GTTTGCCTTG 
GTAGAGGAGA AGGTCCTCTG TAGAGTGCAC TTTGACTGCA TGCTGGACAA TCTGAAGAGA 
GAAGTGGAGA ACGGTAATGG GATTAGTGTG GAAGGAGCCC TTCTCACAGA ACAAGACGTC 
AATCATCCAA AGCCAGCCAA AAGAGCTCGG ACCAGCTTCA CAGCCGATCA GCTCCAGGTT 
ATGCAAGCAC AGTTTGCTCA GGACAACAAC CCAGACGCAC AGACCCTCCA GAAACTGGCA 
GAAAGGACAG GCTTAAGCCG GCGAGTCATA CAGGTGTGGT TTCAGAACTG CAGGGCTCGT 
CATAAGAAAC ATGTCAGTCC CAACCACTCC TCCTCTGCCC CGGTCACAGC AGTCCCCTCA 
TCCAGGCTGT CTCCACCCAT GTTGGAAGAA ATGGCTTATT CCGCCTACGT GCCCCAGGAC 
GGGACGATGC TGACGGCGCT GCATTCATAC ATGGACGCTC ACCAACAACT CCTGGACTCC 
AGCCCTTGCT ACCCCATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest in PC12 cell line.
In vitro cellular & developmental biology. Animal51(4)329-35(2015 Apr)
Li H,Qin J,Jin G,Zou L,Shi J,Han X,Cheng X,Zhang X

LIM homeobox 8 (Lhx8) is a key regulator of the cholinergic neuronal function via a tropomyosin receptor kinase A (TrkA)-mediated positive feedback loop.
The Journal of biological chemistry289(2)1000-10(2014 Jan)
Tomioka T,Shimazaki T,Yamauchi T,Oki T,Ohgoh M,Okano H

LIM-homeobox gene Lhx8 promote the differentiation of hippocampal newborn neurons into cholinergic neurons in vitro.
In vitro cellular & developmental biology. Animal49(2)103-7(2013 Feb)
Zhu P,Li H,Jin G,Tian M,Tan X,Shi J,Zou L,Qin J

Lhx8 promote differentiation of hippocampal neural stem/progenitor cells into cholinergic neurons in vitro.
In vitro cellular & developmental biology. Animal48(10)603-9(2012 Dec)
Shi J,Li H,Jin G,Zhu P,Tian M,Qin J,Tan X,Zhao S,Wang F,Hua Y,Xiao Y

Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression.
Biology of reproduction79(6)1176-82(2008 Dec)
Choi Y,Yuan D,Rajkovic A

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Lhx8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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