Selenot cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenot
Entrez Gene ID	365802
Full Name	selenoprotein T
General protein information	Preferred Names selenoprotein T Names thioredoxin reductase-like selenoprotein T thioredoxin reductase-like enzyme
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q26
Summary	This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif found in several redox active proteins. Studies in rat indicate a role for this gene in the regulation of calcium homeostasis and smooth muscle contraction. High induction of this gene is also associated with ontogenetic and regenerative processes in nervous, endocrine, and metabolic tissues. A pseudogene of this locus has been identified on chromosome 4. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014253.3	NP_001014275.2	thioredoxin reductase-like selenoprotein T precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SELT	NP_001096573.2
Mus musculus (house mouse)	Selt	NP_001035486.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009202	XP_319979.4
Canis lupus familiaris (dog)	SELT	NP_001157959.1
Homo sapiens (human)	SELT	NP_057359.2
Danio rerio (zebrafish)	selt1a	NP_840075.2
Rattus norvegicus (Norway rat)	Selt	NP_001014275.2
Gallus gallus (chicken)	SELT	NP_001006557.3
Xenopus tropicalis (tropical clawed frog)	selt	NP_988868.2
	SELT	NP_001152886.1
Drosophila melanogaster (fruit fly)	CG3887	NP_608897.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Se Enhances MLCK Activation by Regulating Selenoprotein T (SelT) in the Gastric Smooth Muscle of Rats.
Li JP, Zhou JX, Wang Q, Gu GQ, Yang SJ, Li CY, Qiu CW, Deng GZ, Guo MY
Biological trace element research173(1)116-25(2016 Sep)

Selenoprotein T Exerts an Essential Oxidoreductase Activity That Protects Dopaminergic Neurons in Mouse Models of Parkinson's Disease.
Boukhzar L, Hamieh A, Cartier D, Tanguy Y, Alsharif I, Castex M, Arabo A, El Hajji S, Bonnet JJ, Errami M, Falluel-Morel A, Chagraoui A, Lihrmann I, Anouar Y
Antioxidants & redox signaling24(11)557-74(2016 Apr)

Effects of Se on the Diversity of SelT Synthesis and Distribution in Different Smooth Muscle Tissues in Rats.
Guo M, Gao X, Zhang N, Qiu C, Li C, Deng G
Biological trace element research170(2)340-7(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Se plays a major role in regulating the contraction processes of gastric smooth muscle with the SelT.
Title: Se Enhances MLCK Activation by Regulating Selenoprotein T (SelT) in the Gastric Smooth Muscle of Rats.

SelT may play a major role in the mechanisms underlying the biological activity of Se in smooth muscle tissues.
Title: Effects of Se on the Diversity of SelT Synthesis and Distribution in Different Smooth Muscle Tissues in Rats.

SelT induction is associated with ontogenesis, tissue maturation, and regenerative mechanisms, indicating that this PACAP-regulated selenoprotein may play a crucial role in cell growth and activity in nervous, endocrine, and metabolic tissues.
Title: The PACAP-regulated gene selenoprotein T is highly induced in nervous, endocrine, and metabolic tissues during ontogenetic and regenerative processes.

role in the regulation of Ca(2+) homeostasis and neuroendocrine secretion in response to a cAMP-stimulating trophic factor.
Title: Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca2+ mobilization and neuroendocrine secretion.

The following Selenot gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenot cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13317	NM_001014253.3 Latest version!	Rattus norvegicus selenoprotein T (Selenot), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenot ( NM_001014253.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014275.2 Rattus norvegicus selenoprotein T (Selenot), mRNA.

Price & Availability↑

CloneID	ORa13317
Clone ID Related Accession (Same CDS sequence)	NM_001014253.3
Accession Version	NM_001014253.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Rattus norvegicus(Norway rat)
Product	thioredoxin reductase-like selenoprotein T precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112531.5, FM042340.1 and FM061880.1. On Apr 19, 2017 this sequence version replaced NM_001014253.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FQ211813.1, AY995234.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGGCTCT TGCTGCTTCT GCTGGTGGCG GCGTCGGCGG TGGTCCGCAG CGAGGCCTCT 
GCCAACCTGG GCGGCGTGCC CAGCAAGAGA TTAAAGATGC AGTACGCCAC GGGGCCGCTG 
CTCAAGTTTC AGATTTGTGT ATCCTGAGGG TACAGGCGGG TGTTTGAGGA GTACATGCGG 
GTTATTAGCC AGCGGTATCC AGACATCCGC ATTGAGGGGG AGAATTATCT CCCTCAACCA 
ATTTATAGAC ACATAGCATC TTTCCTGTCA GTCTTCAAAC TAGTATTAAT AGGCTTAATA 
ATTGTTGGCA AAGATCCTTT TGCTTTTTTC GGCATGCAAG CTCCTAGCAT CTGGCAGTGG 
GGCCAAGAAA ATAAGGTTTA TGCATGTATG ATGGTCTTCT TCTTGAGCAA CATGATTGAG 
AACCAGTGTA TGTCAACAGG TGCATTTGAG ATAACTTTAA ATGATGTACC GGTATGGTCT 
AAGCTGGAAT CTGGACATCT TCCATCCATG CAACAACTTG TTCAAATTCT TGACAATGAA 
ATGAAACTCA ATGTGCATAT GGATTCAATC CCACATCATC GATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014275.2
CDS	68..655
Translation	MRLLLLLLVAASAVVRSEASANLGGVPSKRLKMQYATGPLLKFQICVS*GYRRVFEEYMRVISQRYPDIRIEGENYLPQPIYRHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFFLSNMIENQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDSIPHHRS

Target ORF information:

RefSeq Version	NM_001014253.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus selenoprotein T (Selenot), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014253.3

ORF Insert Sequence:

ATGAGGCTCT TGCTGCTTCT GCTGGTGGCG GCGTCGGCGG TGGTCCGCAG CGAGGCCTCT 
GCCAACCTGG GCGGCGTGCC CAGCAAGAGA TTAAAGATGC AGTACGCCAC GGGGCCGCTG 
CTCAAGTTTC AGATTTGTGT ATCCTGAGGG TACAGGCGGG TGTTTGAGGA GTACATGCGG 
GTTATTAGCC AGCGGTATCC AGACATCCGC ATTGAGGGGG AGAATTATCT CCCTCAACCA 
ATTTATAGAC ACATAGCATC TTTCCTGTCA GTCTTCAAAC TAGTATTAAT AGGCTTAATA 
ATTGTTGGCA AAGATCCTTT TGCTTTTTTC GGCATGCAAG CTCCTAGCAT CTGGCAGTGG 
GGCCAAGAAA ATAAGGTTTA TGCATGTATG ATGGTCTTCT TCTTGAGCAA CATGATTGAG 
AACCAGTGTA TGTCAACAGG TGCATTTGAG ATAACTTTAA ATGATGTACC GGTATGGTCT 
AAGCTGGAAT CTGGACATCT TCCATCCATG CAACAACTTG TTCAAATTCT TGACAATGAA 
ATGAAACTCA ATGTGCATAT GGATTCAATC CCACATCATC GATCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Se Enhances MLCK Activation by Regulating Selenoprotein T (SelT) in the Gastric Smooth Muscle of Rats.
Biological trace element research173(1)116-25(2016 Sep)
Li JP,Zhou JX,Wang Q,Gu GQ,Yang SJ,Li CY,Qiu CW,Deng GZ,Guo MY

Selenoprotein T Exerts an Essential Oxidoreductase Activity That Protects Dopaminergic Neurons in Mouse Models of Parkinson's Disease.
Antioxidants & redox signaling24(11)557-74(2016 Apr)
Boukhzar L,Hamieh A,Cartier D,Tanguy Y,Alsharif I,Castex M,Arabo A,El Hajji S,Bonnet JJ,Errami M,Falluel-Morel A,Chagraoui A,Lihrmann I,Anouar Y

Effects of Se on the Diversity of SelT Synthesis and Distribution in Different Smooth Muscle Tissues in Rats.
Biological trace element research170(2)340-7(2016 Apr)
Guo M,Gao X,Zhang N,Qiu C,Li C,Deng G

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Selenot cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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