Trpm5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trpm5
Entrez Gene ID	365391
Full Name	transient receptor potential cation channel, subfamily M, member 5
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 5 Names transient receptor potential cation channel subfamily M member 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589501.1	XP_017444990.1	transient receptor potential cation channel subfamily M member 5 isoform X1
XM_008760160.2	XP_008758382.1	transient receptor potential cation channel subfamily M member 5 isoform X2
XM_017589502.1	XP_017444991.1	transient receptor potential cation channel subfamily M member 5 isoform X3
NM_001191896.1	NP_001178825.1	transient receptor potential cation channel subfamily M member 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRPM5	XP_521720.4
Mus musculus (house mouse)	Trpm5	NP_064673.2
Rattus norvegicus (Norway rat)	Trpm5	NP_001178825.1
Homo sapiens (human)	TRPM5	NP_055370.1
	TRPM5	XP_001093334.1
Bos taurus (cattle)	TRPM5	XP_002699492.2
Gallus gallus (chicken)	TRPM5	XP_003641369.2
Canis lupus familiaris (dog)	TRPM5	XP_005631408.1
Danio rerio (zebrafish)	trpm5	NP_001121711.1

Gene Ontology
Bibliography

Related articles in PubMed

Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs).
Qian J, Mummalaneni S, Grider JR, Damaj MI, Lyall V
PloS one13(1)e0190465(2018)

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
Feng R, Qian C, Liu Q, Jin Y, Liu L, Li S, Liao Y, Zhou H, Liu W, Rayner CK, Ma J
General and comparative endocrinology252142-149(2017 Oct)

Role of transient receptor potential melastatin-like subtype 5 channel in insulin secretion from rat ?-cells.
Krishnan K, Ma Z, Bj?rklund A, Islam MS
Pancreas43(4)597-604(2014 May)

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response.
Ren Z, Rhyu MR, Phan TH, Mummalaneni S, Murthy KS, Grider JR, DeSimone JA, Lyall V
American journal of physiology. Gastrointestinal and liver physiology305(1)G106-17(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sweet taste molecules of T1R2, T1R3, alpha-gustducin and TRPM5 in ileum were dramatically higher than those in duodenum (P<0.01 for each).
Title: Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.

In rat pancreatic islets, TRPM5 is involved in mediating insulin secretion by glucose and l-arginine and in potentiating the glucose-induced insulin secretion by glucagon-like peptide 1.
Title: Role of transient receptor potential melastatin-like subtype 5 channel in insulin secretion from rat ?-cells.

The results suggest that pharmacological or genetic alteration of TRPM5 activity modulates the benzamil-insensitive NaCl chorda tympani response and its modulation by TRPV1 agonists.
Title: TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response.

These channels may play an important role in determining the distinct firing properties of vasopressin neurones in the supraoptic nucleus.
Title: Transient receptor potential channel m4 and m5 in magnocellular cells in rat supraoptic and paraventricular nuclei.

The following Trpm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trpm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07959	NM_001191896.1 Latest version!	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa56761	XM_017589501.1 Latest version!	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa56762	XM_017589502.1 Latest version!	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa48727	XM_008760160.2 Latest version!	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trpm5 ( NM_001191896.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178825.1 Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.

Price & Availability↑

CloneID	ORa07959
Clone ID Related Accession (Same CDS sequence)	NM_001191896.1
Accession Version	NM_001191896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-26
Organism	Rattus norvegicus(Norway rat)
Product	transient receptor potential cation channel subfamily M member 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC095135.11. On Jul 17, 2010 this sequence version replaced XM_344979.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCCGATGG CCCAGAGCTC TTGTCCTGGA AGCCCCCCAG ATACTGGGGA TGGATGGGAG 
CCAGTCCTAT GCAAGGGAGA GGTCAACTTC GGAGGGTCTG GGAAAAAGCG AAGCAAGTTT 
GTGAAGGTGC CAAGCAATGT GGCCCCCTCC ATGCTCTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGCG AGGAACGGCT TTTTGCTATG 
AAGTCCTGGC TTCGGGATGT CTTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCATGTG GGCCTGGCAC GCCATGTTGG ACAGGCTGTA 
CGTGATCACT CTCTGGCTAG CACGTCCACC AAGGTCCGTG TGGTGGCCAT CGGAATGGCC 
TCTCTGGACC GAATCCTTCA CCGCCAACTT CTAGATGGTG TCCAGGAGGA TACTCCCATC 
CACTACCCAG CAGATGAGGG CAGCACTCAG GGACCCCTCT GCCCTCTGGA CAGCAATCTC 
TCCCACTTCA TCCTCGTGGA GCCAGGCACC CTTGGGAGTG GGAACGACGG ACTGGCAGAG 
CTGCAGCTGA GCCTGGAGAA GCACATCTCT CAGCAGAGGA CAGGTTATGG GGGTACCAGC 
AGCATCCAGA TACCTGTCCT TTGCTTGCTA GTCAATGGTG ACCCCAGCAC CCTAGAGAGG 
ATGTCCAGGG CAGTGGAGCA GGCTGCCCCA TGGCTGATCC TGGCAGGTTC TGGGGGCATT 
GCTGATGTAC TCGCTGCCCT GGTGGGCCAG CCTCATCTCC TGGTGCCCCA GGTGACCGAG 
AAGCAGTTCA GAGAGAAATT CCCAAGCGAG TGTTTCTCTT GGGAAGCCAT TGTACACTGG 
ACAGAGCTGC TACAGAACAT TGCTGCACAC CCCCACCTGC TCACAGTGTA CGACTTTGAG 
CAGGAGGGTT CCGAGGACCT GGACACCGTC ATCCTCAAGG CACTTGTGAA AGCCTGCAAG 
AGTCACAGCC GAGACGCACA AGACTACCTA GATGAGCTCA AGTTAGCAGT GGCCTGGGAT 
CGCGTGGACA TTGCCAAGAG TGAAATCTTC AATGGGGACG TGGAGTGGAA GTCCTGTGAC 
TTGGAAGAGG TGATGACAGA TGCCCTAGTG AGCAACAAGC CTGACTTCGT GCGCCTCTTT 
GTGGACAGTG GTGCTGACAT GGCCGAGTTC TTGACCTATG GGCGGCTGCA GCAGCTTTAC 
CACTCTGTGT CCCCCAAGAG CCTCCTCTTT GAACTGCTGG AGCGTAAGCA TGAGGAGGGT 
CGGCTGACAC TGGCTGGCCT GGGTGCCCAG CAGACCCGGG AGCTGCCCGT TGGTCTGCCT 
GCCTTTTCAC TCCATGAGGT CTCCCGAGTT CTCAAAGATT TCCTGCATGA CGCCTGCCGT 
GGCTTCTACC AGGATGGGCG CAGGATGGAG GAGAGAGGGC CACCCAAGCG GCCTGCAGGC 
CAGAAATGGC TGCCGGACCT CAGTCGGAAG AGTGAAGACC CATGGAGGGA CCTGTTCCTT 
TGGGCTGTGC TGCAGAACCG TTATGAGATG GCCACATACT TCTGGGCCAT GGGCCGGGAG 
GGTGTGGCTG CTGCTCTGGC GGCCTGCAAG ATCATCAAGG AAATGTCCCA CCTGGAGAAA 
GAGGCAGAGG TGGCCCGCAC TATGCGTGAG GCCAAGTATG AGCAGCTGGC CCTCGATCTT 
TTCTCAGAGT GCTACAGCAA CAGTGAGGAC CGTGCCTTTG CCCTGTTGGT GCGCAGGAAC 
CACAGCTGGA GCAGGACCAC CTGCCTGCAC CTGGCCACTG AGGCCGATGC CAAGGCCTTC 
TTTGCCCATG ATGGTGTGCA AGCATTCCTG ACGAAGATCT GGTGGGGAGA CATGGCCACA 
GGCACACCCA TCTTACGACT TCTGGGTGCC TTCACCTGCC CAGCCCTCAT CTACACAAAT 
CTCATCTCCT TCAGTGAGGA TGCCCCGCAG AGGATGGACC TGGAAGATCT GCAGGAGCCA 
GACAGTTTGG ATATGGAAAA GAGCTTCCTG TGCAGCCATG GTGGCCAATT GGAGAAGTTA 
ACAGAGGCGC CAAGGGCTCC TGGCGATCTA GGCCCACAAG CTGCCTTCCT GCTCACACGG 
TGGAGGAAGT TCTGGGGCGC TCCTGTGACT GTGTTCTTGG GGAATGTGGT CATGTACTTT 
GCATTCCTCT TCCTATTCTC CTACGTCCTG CTGGTGGATT TCAGGCCACC ACCCCAGGGG 
CCATCTGGGT CGGAAGTTAC CCTGTATTTC TGGGTCTTCA CACTGGTGCT GGAGGAAATC 
CGACAGGGAT TCTTCACAAA CGAGGACACC CGTCTGGTGA AGAAGTTCAC TCTGTACGTA 
GAAGACAACT GGAACAAATG TGACATGGTG GCCATCTTCC TGTTCATTGT TGGTGTCACC 
TGTAGAATGG TGCCCTCCGT GTTTGAGGCT GGCCGGACTG TTCTGGCCAT TGACTTCATG 
GTGTTCACAC TTCGGCTCAT CCACATCTTT GCTATTCACA AGCAGCTGGG TCCTAAGATC 
ATCATTGTAG AGCGGATGAT GAAAGATGTC TTCTTCTTCC TCTTCTTCCT GAGCGTGTGG 
CTCGTGGCCT ATGGCGTGAC CACTCAGGCC CTGCTGCACC CCCACGATGG CCGTCTGGAG 
TGGATTTTCC GCCGTGTGCT CTACAGGCCT TACCTGCAGA TCTTTGGGCA AATCCCTCTG 
GATGAAATTG ATGAGGCCCG TGTGAACTGC TCTCTTCACC CGTTGCTGCT GGACAGCTCA 
GCTTCCTGCC CTAATCTCTA TGCCAACTGG CTGGTCATTC TCCTGCTGGT TACCTTCCTC 
CTCGTCACTA ATGTGCTACT TATGAACCTT CTGATCGCCA TGTTCAGCTA CACATTCCAG 
GTGGTGCAGG GCAATGCAGA CATGTTCTGG AAGTTTCAAC GCTACCACCT CATCGTTGAA 
TACCACGGAA GGCCGGCTCT GGCCCCGCCC TTCATCCTGC TCAGCCACCT GAGCCTGGTG 
CTCAAGCAGG TCTTCAGGAA GGAAGCCCAG CACAAACAGC AACACCTGGA GAGAGACTTG 
CCTGACCCCG TGGACCAGAA GATCATTACC TGGGAAACAG TTCAAAAGGA GAACTTCCTG 
AGTACCATGG AGAAACGGAG GAGGGACAGT GAGGAGGAGG TGCTGAGGAA AACGGCACAC 
AGAGTGGACT TGATTGCCAA ATACATCGGG GGTCTGAGAG AGCAAGAAAA GAGGATCAAG 
TGTCTGGAGT CACAGGCCAA CTACTGTATG CTCCTCTTGT CCTCCATGAC TGACACACTG 
GCTCCTGGAG GCACCTACTC AAGTTCTCAA AACTGTGGTC GCAGGAGTCA GCCAGCCTCT 
GCTAGAGACA GGGAGTACCT AGAGGCTGGC TTGCCACACT CAGACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178825.1
CDS	1..3471
Translation	MPMAQSSCPGSPPDTGDGWEPVLCKGEVNFGGSGKKRSKFVKVPSNVAPSMLFELLLTEWHLPAPNLVVSLVGEERLFAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKVRVVAIGMASLDRILHRQLLDGVQEDTPIHYPADEGSTQGPLCPLDSNLSHFILVEPGTLGSGNDGLAELQLSLEKHISQQRTGYGGTSSIQIPVLCLLVNGDPSTLERMSRAVEQAAPWLILAGSGGIADVLAALVGQPHLLVPQVTEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSRDAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLERKHEEGRLTLAGLGAQQTRELPVGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYSNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSHGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFSYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTNEDTRLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLDSSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKQQHLERDLPDPVDQKIITWETVQKENFLSTMEKRRRDSEEEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGRRSQPASARDREYLEAGLPHSDT

Target ORF information:

RefSeq Version	NM_001191896.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191896.1

ORF Insert Sequence:

ATGCCGATGG CCCAGAGCTC TTGTCCTGGA AGCCCCCCAG ATACTGGGGA TGGATGGGAG 
CCAGTCCTAT GCAAGGGAGA GGTCAACTTC GGAGGGTCTG GGAAAAAGCG AAGCAAGTTT 
GTGAAGGTGC CAAGCAATGT GGCCCCCTCC ATGCTCTTTG AACTCCTGCT CACCGAGTGG 
CACCTGCCAG CCCCCAACCT GGTGGTGTCC CTGGTGGGCG AGGAACGGCT TTTTGCTATG 
AAGTCCTGGC TTCGGGATGT CTTGCGCAAG GGGCTGGTGA AAGCAGCTCA GAGCACAGGT 
GCCTGGATCC TGACCAGTGC CCTCCATGTG GGCCTGGCAC GCCATGTTGG ACAGGCTGTA 
CGTGATCACT CTCTGGCTAG CACGTCCACC AAGGTCCGTG TGGTGGCCAT CGGAATGGCC 
TCTCTGGACC GAATCCTTCA CCGCCAACTT CTAGATGGTG TCCAGGAGGA TACTCCCATC 
CACTACCCAG CAGATGAGGG CAGCACTCAG GGACCCCTCT GCCCTCTGGA CAGCAATCTC 
TCCCACTTCA TCCTCGTGGA GCCAGGCACC CTTGGGAGTG GGAACGACGG ACTGGCAGAG 
CTGCAGCTGA GCCTGGAGAA GCACATCTCT CAGCAGAGGA CAGGTTATGG GGGTACCAGC 
AGCATCCAGA TACCTGTCCT TTGCTTGCTA GTCAATGGTG ACCCCAGCAC CCTAGAGAGG 
ATGTCCAGGG CAGTGGAGCA GGCTGCCCCA TGGCTGATCC TGGCAGGTTC TGGGGGCATT 
GCTGATGTAC TCGCTGCCCT GGTGGGCCAG CCTCATCTCC TGGTGCCCCA GGTGACCGAG 
AAGCAGTTCA GAGAGAAATT CCCAAGCGAG TGTTTCTCTT GGGAAGCCAT TGTACACTGG 
ACAGAGCTGC TACAGAACAT TGCTGCACAC CCCCACCTGC TCACAGTGTA CGACTTTGAG 
CAGGAGGGTT CCGAGGACCT GGACACCGTC ATCCTCAAGG CACTTGTGAA AGCCTGCAAG 
AGTCACAGCC GAGACGCACA AGACTACCTA GATGAGCTCA AGTTAGCAGT GGCCTGGGAT 
CGCGTGGACA TTGCCAAGAG TGAAATCTTC AATGGGGACG TGGAGTGGAA GTCCTGTGAC 
TTGGAAGAGG TGATGACAGA TGCCCTAGTG AGCAACAAGC CTGACTTCGT GCGCCTCTTT 
GTGGACAGTG GTGCTGACAT GGCCGAGTTC TTGACCTATG GGCGGCTGCA GCAGCTTTAC 
CACTCTGTGT CCCCCAAGAG CCTCCTCTTT GAACTGCTGG AGCGTAAGCA TGAGGAGGGT 
CGGCTGACAC TGGCTGGCCT GGGTGCCCAG CAGACCCGGG AGCTGCCCGT TGGTCTGCCT 
GCCTTTTCAC TCCATGAGGT CTCCCGAGTT CTCAAAGATT TCCTGCATGA CGCCTGCCGT 
GGCTTCTACC AGGATGGGCG CAGGATGGAG GAGAGAGGGC CACCCAAGCG GCCTGCAGGC 
CAGAAATGGC TGCCGGACCT CAGTCGGAAG AGTGAAGACC CATGGAGGGA CCTGTTCCTT 
TGGGCTGTGC TGCAGAACCG TTATGAGATG GCCACATACT TCTGGGCCAT GGGCCGGGAG 
GGTGTGGCTG CTGCTCTGGC GGCCTGCAAG ATCATCAAGG AAATGTCCCA CCTGGAGAAA 
GAGGCAGAGG TGGCCCGCAC TATGCGTGAG GCCAAGTATG AGCAGCTGGC CCTCGATCTT 
TTCTCAGAGT GCTACAGCAA CAGTGAGGAC CGTGCCTTTG CCCTGTTGGT GCGCAGGAAC 
CACAGCTGGA GCAGGACCAC CTGCCTGCAC CTGGCCACTG AGGCCGATGC CAAGGCCTTC 
TTTGCCCATG ATGGTGTGCA AGCATTCCTG ACGAAGATCT GGTGGGGAGA CATGGCCACA 
GGCACACCCA TCTTACGACT TCTGGGTGCC TTCACCTGCC CAGCCCTCAT CTACACAAAT 
CTCATCTCCT TCAGTGAGGA TGCCCCGCAG AGGATGGACC TGGAAGATCT GCAGGAGCCA 
GACAGTTTGG ATATGGAAAA GAGCTTCCTG TGCAGCCATG GTGGCCAATT GGAGAAGTTA 
ACAGAGGCGC CAAGGGCTCC TGGCGATCTA GGCCCACAAG CTGCCTTCCT GCTCACACGG 
TGGAGGAAGT TCTGGGGCGC TCCTGTGACT GTGTTCTTGG GGAATGTGGT CATGTACTTT 
GCATTCCTCT TCCTATTCTC CTACGTCCTG CTGGTGGATT TCAGGCCACC ACCCCAGGGG 
CCATCTGGGT CGGAAGTTAC CCTGTATTTC TGGGTCTTCA CACTGGTGCT GGAGGAAATC 
CGACAGGGAT TCTTCACAAA CGAGGACACC CGTCTGGTGA AGAAGTTCAC TCTGTACGTA 
GAAGACAACT GGAACAAATG TGACATGGTG GCCATCTTCC TGTTCATTGT TGGTGTCACC 
TGTAGAATGG TGCCCTCCGT GTTTGAGGCT GGCCGGACTG TTCTGGCCAT TGACTTCATG 
GTGTTCACAC TTCGGCTCAT CCACATCTTT GCTATTCACA AGCAGCTGGG TCCTAAGATC 
ATCATTGTAG AGCGGATGAT GAAAGATGTC TTCTTCTTCC TCTTCTTCCT GAGCGTGTGG 
CTCGTGGCCT ATGGCGTGAC CACTCAGGCC CTGCTGCACC CCCACGATGG CCGTCTGGAG 
TGGATTTTCC GCCGTGTGCT CTACAGGCCT TACCTGCAGA TCTTTGGGCA AATCCCTCTG 
GATGAAATTG ATGAGGCCCG TGTGAACTGC TCTCTTCACC CGTTGCTGCT GGACAGCTCA 
GCTTCCTGCC CTAATCTCTA TGCCAACTGG CTGGTCATTC TCCTGCTGGT TACCTTCCTC 
CTCGTCACTA ATGTGCTACT TATGAACCTT CTGATCGCCA TGTTCAGCTA CACATTCCAG 
GTGGTGCAGG GCAATGCAGA CATGTTCTGG AAGTTTCAAC GCTACCACCT CATCGTTGAA 
TACCACGGAA GGCCGGCTCT GGCCCCGCCC TTCATCCTGC TCAGCCACCT GAGCCTGGTG 
CTCAAGCAGG TCTTCAGGAA GGAAGCCCAG CACAAACAGC AACACCTGGA GAGAGACTTG 
CCTGACCCCG TGGACCAGAA GATCATTACC TGGGAAACAG TTCAAAAGGA GAACTTCCTG 
AGTACCATGG AGAAACGGAG GAGGGACAGT GAGGAGGAGG TGCTGAGGAA AACGGCACAC 
AGAGTGGACT TGATTGCCAA ATACATCGGG GGTCTGAGAG AGCAAGAAAA GAGGATCAAG 
TGTCTGGAGT CACAGGCCAA CTACTGTATG CTCCTCTTGT CCTCCATGAC TGACACACTG 
GCTCCTGGAG GCACCTACTC AAGTTCTCAA AACTGTGGTC GCAGGAGTCA GCCAGCCTCT 
GCTAGAGACA GGGAGTACCT AGAGGCTGGC TTGCCACACT CAGACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_017589501.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444990.1 Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56761
Clone ID Related Accession (Same CDS sequence)	XM_017589501.1
Accession Version	XM_017589501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transient receptor potential cation channel subfamily M member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG TCTGGAAGGA ACAGACAGGA GACCCTCTGC AAAAGCCTCT GGAGAGCTGT 
GTCGAGGGTG TTGGAATCCA GATGCCCGGA GTTCGAAAAG TCACAATGCC GATGGCCCAG 
AGCTCTTGTC CTGGAAGCCC CCCAGATACT GGGGATGGAT GGGAGCCAGT CCTATGCAAG 
GGAGAGGTCA ACTTCGGAGG GTCTGGGAAA AAGCGAAGCA AGTTTGTGAA GGTGCCAAGC 
AATGTGGCCC CCTCCATGCT CTTTGAACTC CTGCTCACCG AGTGGCACCT GCCAGCCCCC 
AACCTGGTGG TGTCCCTGGT GGGCGAGGAA CGGCTTTTTG CTATGAAGTC CTGGCTTCGG 
GATGTCTTGC GCAAGGGGCT GGTGAAAGCA GCTCAGAGCA CAGGTGCCTG GATCCTGACC 
AGTGCCCTCC ATGTGGGCCT GGCACGCCAT GTTGGACAGG CTGTACGTGA TCACTCTCTG 
GCTAGCACGT CCACCAAGGT CCGTGTGGTG GCCATCGGAA TGGCCTCTCT GGACCGAATC 
CTTCACCGCC AACTTCTAGA TGGTGTCCAG GAGGATACTC CCATCCACTA CCCAGCAGAT 
GAGGGCAGCA CTCAGGGACC CCTCTGCCCT CTGGACAGCA ATCTCTCCCA CTTCATCCTC 
GTGGAGCCAG GCACCCTTGG GAGTGGGAAC GACGGACTGG CAGAGCTGCA GCTGAGCCTG 
GAGAAGCACA TCTCTCAGCA GAGGACAGGT TATGGGGGTA CCAGCAGCAT CCAGATACCT 
GTCCTTTGCT TGCTAGTCAA TGGTGACCCC AGCACCCTAG AGAGGATGTC CAGGGCAGTG 
GAGCAGGCTG CCCCATGGCT GATCCTGGCA GGTTCTGGGG GCATTGCTGA TGTACTCGCT 
GCCCTGGTGG GCCAGCCTCA TCTCCTGGTG CCCCAGGTGA CCGAGAAGCA GTTCAGAGAG 
AAATTCCCAA GCGAGTGTTT CTCTTGGGAA GCCATTGTAC ACTGGACAGA GCTGCTACAG 
AACATTGCTG CACACCCCCA CCTGCTCACA GTGTACGACT TTGAGCAGGA GGGTTCCGAG 
GACCTGGACA CCGTCATCCT CAAGGCACTT GTGAAAGCCT GCAAGAGTCA CAGCCGAGAC 
GCACAAGACT ACCTAGATGA GCTCAAGTTA GCAGTGGCCT GGGATCGCGT GGACATTGCC 
AAGAGTGAAA TCTTCAATGG GGACGTGGAG TGGAAGTCCT GTGACTTGGA AGAGGTGATG 
ACAGATGCCC TAGTGAGCAA CAAGCCTGAC TTCGTGCGCC TCTTTGTGGA CAGTGGTGCT 
GACATGGCCG AGTTCTTGAC CTATGGGCGG CTGCAGCAGC TTTACCACTC TGTGTCCCCC 
AAGAGCCTCC TCTTTGAACT GCTGGAGCGT AAGCATGAGG AGGGTCGGCT GACACTGGCT 
GGCCTGGGTG CCCAGCAGAC CCGGGAGCTG CCCGTTGGTC TGCCTGCCTT TTCACTCCAT 
GAGGTCTCCC GAGTTCTCAA AGATTTCCTG CATGACGCCT GCCGTGGCTT CTACCAGGAT 
GGGCGCAGGA TGGAGGAGAG AGGGCCACCC AAGCGGCCTG CAGGCCAGAA ATGGCTGCCG 
GACCTCAGTC GGAAGAGTGA AGACCCATGG AGGGACCTGT TCCTTTGGGC TGTGCTGCAG 
AACCGTTATG AGATGGCCAC ATACTTCTGG GCCATGGGCC GGGAGGGTGT GGCTGCTGCT 
CTGGCGGCCT GCAAGATCAT CAAGGAAATG TCCCACCTGG AGAAAGAGGC AGAGGTGGCC 
CGCACTATGC GTGAGGCCAA GTATGAGCAG CTGGCCCTCG ATCTTTTCTC AGAGTGCTAC 
AGCAACAGTG AGGACCGTGC CTTTGCCCTG TTGGTGCGCA GGAACCACAG CTGGAGCAGG 
ACCACCTGCC TGCACCTGGC CACTGAGGCC GATGCCAAGG CCTTCTTTGC CCATGATGGT 
GTGCAAGCAT TCCTGACGAA GATCTGGTGG GGAGACATGG CCACAGGCAC ACCCATCTTA 
CGACTTCTGG GTGCCTTCAC CTGCCCAGCC CTCATCTACA CAAATCTCAT CTCCTTCAGT 
GAGGATGCCC CGCAGAGGAT GGACCTGGAA GATCTGCAGG AGCCAGACAG TTTGGATATG 
GAAAAGAGCT TCCTGTGCAG CCATGGTGGC CAATTGGAGA AGTTAACAGA GGCGCCAAGG 
GCTCCTGGCG ATCTAGGCCC ACAAGCTGCC TTCCTGCTCA CACGGTGGAG GAAGTTCTGG 
GGCGCTCCTG TGACTGTGTT CTTGGGGAAT GTGGTCATGT ACTTTGCATT CCTCTTCCTA 
TTCTCCTACG TCCTGCTGGT GGATTTCAGG CCACCACCCC AGGGGCCATC TGGGTCGGAA 
GTTACCCTGT ATTTCTGGGT CTTCACACTG GTGCTGGAGG AAATCCGACA GGGATTCTTC 
ACAAACGAGG ACACCCGTCT GGTGAAGAAG TTCACTCTGT ACGTAGAAGA CAACTGGAAC 
AAATGTGACA TGGTGGCCAT CTTCCTGTTC ATTGTTGGTG TCACCTGTAG AATGGTGCCC 
TCCGTGTTTG AGGCTGGCCG GACTGTTCTG GCCATTGACT TCATGGTGTT CACACTTCGG 
CTCATCCACA TCTTTGCTAT TCACAAGCAG CTGGGTCCTA AGATCATCAT TGTAGAGCGG 
ATGATGAAAG ATGTCTTCTT CTTCCTCTTC TTCCTGAGCG TGTGGCTCGT GGCCTATGGC 
GTGACCACTC AGGCCCTGCT GCACCCCCAC GATGGCCGTC TGGAGTGGAT TTTCCGCCGT 
GTGCTCTACA GGCCTTACCT GCAGATCTTT GGGCAAATCC CTCTGGATGA AATTGATGAG 
GCCCGTGTGA ACTGCTCTCT TCACCCGTTG CTGCTGGACA GCTCAGCTTC CTGCCCTAAT 
CTCTATGCCA ACTGGCTGGT CATTCTCCTG CTGGTTACCT TCCTCCTCGT CACTAATGTG 
CTACTTATGA ACCTTCTGAT CGCCATGTTC AGCTACACAT TCCAGGTGGT GCAGGGCAAT 
GCAGACATGT TCTGGAAGTT TCAACGCTAC CACCTCATCG TTGAATACCA CGGAAGGCCG 
GCTCTGGCCC CGCCCTTCAT CCTGCTCAGC CACCTGAGCC TGGTGCTCAA GCAGGTCTTC 
AGGAAGGAAG CCCAGCACAA ACAGCAACAC CTGGAGAGAG ACTTGCCTGA CCCCGTGGAC 
CAGAAGATCA TTACCTGGGA AACAGTTCAA AAGGAGAACT TCCTGAGTAC CATGGAGAAA 
CGGAGGAGGG ACAGTGAGGA GGAGGTGCTG AGGAAAACGG CACACAGAGT GGACTTGATT 
GCCAAATACA TCGGGGGTCT GAGAGAGCAA GAAAAGAGGA TCAAGTGTCT GGAGTCACAG 
GCCAACTACT GTATGCTCCT CTTGTCCTCC ATGACTGACA CACTGGCTCC TGGAGGCACC 
TACTCAAGTT CTCAAAACTG TGGTCGCAGG AGTCAGCCAG CCTCTGCTAG AGACAGGGAG 
TACCTAGAGG CTGGCTTGCC ACACTCAGAC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444990.1
CDS	2107..5682
Translation	MPGVWKEQTGDPLQKPLESCVEGVGIQMPGVRKVTMPMAQSSCPGSPPDTGDGWEPVLCKGEVNFGGSGKKRSKFVKVPSNVAPSMLFELLLTEWHLPAPNLVVSLVGEERLFAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKVRVVAIGMASLDRILHRQLLDGVQEDTPIHYPADEGSTQGPLCPLDSNLSHFILVEPGTLGSGNDGLAELQLSLEKHISQQRTGYGGTSSIQIPVLCLLVNGDPSTLERMSRAVEQAAPWLILAGSGGIADVLAALVGQPHLLVPQVTEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSRDAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLERKHEEGRLTLAGLGAQQTRELPVGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYSNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSHGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFSYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTNEDTRLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLDSSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKQQHLERDLPDPVDQKIITWETVQKENFLSTMEKRRRDSEEEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGRRSQPASARDREYLEAGLPHSDT

Target ORF information:

RefSeq Version	XM_017589501.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589501.1

ORF Insert Sequence:

ATGCCAGGAG TCTGGAAGGA ACAGACAGGA GACCCTCTGC AAAAGCCTCT GGAGAGCTGT 
GTCGAGGGTG TTGGAATCCA GATGCCCGGA GTTCGAAAAG TCACAATGCC GATGGCCCAG 
AGCTCTTGTC CTGGAAGCCC CCCAGATACT GGGGATGGAT GGGAGCCAGT CCTATGCAAG 
GGAGAGGTCA ACTTCGGAGG GTCTGGGAAA AAGCGAAGCA AGTTTGTGAA GGTGCCAAGC 
AATGTGGCCC CCTCCATGCT CTTTGAACTC CTGCTCACCG AGTGGCACCT GCCAGCCCCC 
AACCTGGTGG TGTCCCTGGT GGGCGAGGAA CGGCTTTTTG CTATGAAGTC CTGGCTTCGG 
GATGTCTTGC GCAAGGGGCT GGTGAAAGCA GCTCAGAGCA CAGGTGCCTG GATCCTGACC 
AGTGCCCTCC ATGTGGGCCT GGCACGCCAT GTTGGACAGG CTGTACGTGA TCACTCTCTG 
GCTAGCACGT CCACCAAGGT CCGTGTGGTG GCCATCGGAA TGGCCTCTCT GGACCGAATC 
CTTCACCGCC AACTTCTAGA TGGTGTCCAG GAGGATACTC CCATCCACTA CCCAGCAGAT 
GAGGGCAGCA CTCAGGGACC CCTCTGCCCT CTGGACAGCA ATCTCTCCCA CTTCATCCTC 
GTGGAGCCAG GCACCCTTGG GAGTGGGAAC GACGGACTGG CAGAGCTGCA GCTGAGCCTG 
GAGAAGCACA TCTCTCAGCA GAGGACAGGT TATGGGGGTA CCAGCAGCAT CCAGATACCT 
GTCCTTTGCT TGCTAGTCAA TGGTGACCCC AGCACCCTAG AGAGGATGTC CAGGGCAGTG 
GAGCAGGCTG CCCCATGGCT GATCCTGGCA GGTTCTGGGG GCATTGCTGA TGTACTCGCT 
GCCCTGGTGG GCCAGCCTCA TCTCCTGGTG CCCCAGGTGA CCGAGAAGCA GTTCAGAGAG 
AAATTCCCAA GCGAGTGTTT CTCTTGGGAA GCCATTGTAC ACTGGACAGA GCTGCTACAG 
AACATTGCTG CACACCCCCA CCTGCTCACA GTGTACGACT TTGAGCAGGA GGGTTCCGAG 
GACCTGGACA CCGTCATCCT CAAGGCACTT GTGAAAGCCT GCAAGAGTCA CAGCCGAGAC 
GCACAAGACT ACCTAGATGA GCTCAAGTTA GCAGTGGCCT GGGATCGCGT GGACATTGCC 
AAGAGTGAAA TCTTCAATGG GGACGTGGAG TGGAAGTCCT GTGACTTGGA AGAGGTGATG 
ACAGATGCCC TAGTGAGCAA CAAGCCTGAC TTCGTGCGCC TCTTTGTGGA CAGTGGTGCT 
GACATGGCCG AGTTCTTGAC CTATGGGCGG CTGCAGCAGC TTTACCACTC TGTGTCCCCC 
AAGAGCCTCC TCTTTGAACT GCTGGAGCGT AAGCATGAGG AGGGTCGGCT GACACTGGCT 
GGCCTGGGTG CCCAGCAGAC CCGGGAGCTG CCCGTTGGTC TGCCTGCCTT TTCACTCCAT 
GAGGTCTCCC GAGTTCTCAA AGATTTCCTG CATGACGCCT GCCGTGGCTT CTACCAGGAT 
GGGCGCAGGA TGGAGGAGAG AGGGCCACCC AAGCGGCCTG CAGGCCAGAA ATGGCTGCCG 
GACCTCAGTC GGAAGAGTGA AGACCCATGG AGGGACCTGT TCCTTTGGGC TGTGCTGCAG 
AACCGTTATG AGATGGCCAC ATACTTCTGG GCCATGGGCC GGGAGGGTGT GGCTGCTGCT 
CTGGCGGCCT GCAAGATCAT CAAGGAAATG TCCCACCTGG AGAAAGAGGC AGAGGTGGCC 
CGCACTATGC GTGAGGCCAA GTATGAGCAG CTGGCCCTCG ATCTTTTCTC AGAGTGCTAC 
AGCAACAGTG AGGACCGTGC CTTTGCCCTG TTGGTGCGCA GGAACCACAG CTGGAGCAGG 
ACCACCTGCC TGCACCTGGC CACTGAGGCC GATGCCAAGG CCTTCTTTGC CCATGATGGT 
GTGCAAGCAT TCCTGACGAA GATCTGGTGG GGAGACATGG CCACAGGCAC ACCCATCTTA 
CGACTTCTGG GTGCCTTCAC CTGCCCAGCC CTCATCTACA CAAATCTCAT CTCCTTCAGT 
GAGGATGCCC CGCAGAGGAT GGACCTGGAA GATCTGCAGG AGCCAGACAG TTTGGATATG 
GAAAAGAGCT TCCTGTGCAG CCATGGTGGC CAATTGGAGA AGTTAACAGA GGCGCCAAGG 
GCTCCTGGCG ATCTAGGCCC ACAAGCTGCC TTCCTGCTCA CACGGTGGAG GAAGTTCTGG 
GGCGCTCCTG TGACTGTGTT CTTGGGGAAT GTGGTCATGT ACTTTGCATT CCTCTTCCTA 
TTCTCCTACG TCCTGCTGGT GGATTTCAGG CCACCACCCC AGGGGCCATC TGGGTCGGAA 
GTTACCCTGT ATTTCTGGGT CTTCACACTG GTGCTGGAGG AAATCCGACA GGGATTCTTC 
ACAAACGAGG ACACCCGTCT GGTGAAGAAG TTCACTCTGT ACGTAGAAGA CAACTGGAAC 
AAATGTGACA TGGTGGCCAT CTTCCTGTTC ATTGTTGGTG TCACCTGTAG AATGGTGCCC 
TCCGTGTTTG AGGCTGGCCG GACTGTTCTG GCCATTGACT TCATGGTGTT CACACTTCGG 
CTCATCCACA TCTTTGCTAT TCACAAGCAG CTGGGTCCTA AGATCATCAT TGTAGAGCGG 
ATGATGAAAG ATGTCTTCTT CTTCCTCTTC TTCCTGAGCG TGTGGCTCGT GGCCTATGGC 
GTGACCACTC AGGCCCTGCT GCACCCCCAC GATGGCCGTC TGGAGTGGAT TTTCCGCCGT 
GTGCTCTACA GGCCTTACCT GCAGATCTTT GGGCAAATCC CTCTGGATGA AATTGATGAG 
GCCCGTGTGA ACTGCTCTCT TCACCCGTTG CTGCTGGACA GCTCAGCTTC CTGCCCTAAT 
CTCTATGCCA ACTGGCTGGT CATTCTCCTG CTGGTTACCT TCCTCCTCGT CACTAATGTG 
CTACTTATGA ACCTTCTGAT CGCCATGTTC AGCTACACAT TCCAGGTGGT GCAGGGCAAT 
GCAGACATGT TCTGGAAGTT TCAACGCTAC CACCTCATCG TTGAATACCA CGGAAGGCCG 
GCTCTGGCCC CGCCCTTCAT CCTGCTCAGC CACCTGAGCC TGGTGCTCAA GCAGGTCTTC 
AGGAAGGAAG CCCAGCACAA ACAGCAACAC CTGGAGAGAG ACTTGCCTGA CCCCGTGGAC 
CAGAAGATCA TTACCTGGGA AACAGTTCAA AAGGAGAACT TCCTGAGTAC CATGGAGAAA 
CGGAGGAGGG ACAGTGAGGA GGAGGTGCTG AGGAAAACGG CACACAGAGT GGACTTGATT 
GCCAAATACA TCGGGGGTCT GAGAGAGCAA GAAAAGAGGA TCAAGTGTCT GGAGTCACAG 
GCCAACTACT GTATGCTCCT CTTGTCCTCC ATGACTGACA CACTGGCTCC TGGAGGCACC 
TACTCAAGTT CTCAAAACTG TGGTCGCAGG AGTCAGCCAG CCTCTGCTAG AGACAGGGAG 
TACCTAGAGG CTGGCTTGCC ACACTCAGAC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_017589502.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444991.1 Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.

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CloneID	ORa56762
Clone ID Related Accession (Same CDS sequence)	XM_017589502.1
Accession Version	XM_017589502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transient receptor potential cation channel subfamily M member 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAG TTCGAAAAGT CACAATGCCG ATGGCCCAGA GCTCTTGTCC TGGAAGCCCC 
CCAGATACTG GGGATGGATG GGAGCCAGTC CTATGCAAGG GAGAGGTCAA CTTCGGAGGG 
TCTGGGAAAA AGCGAAGCAA GTTTGTGAAG GTGCCAAGCA ATGTGGCCCC CTCCATGCTC 
TTTGAACTCC TGCTCACCGA GTGGCACCTG CCAGCCCCCA ACCTGGTGGT GTCCCTGGTG 
GGCGAGGAAC GGCTTTTTGC TATGAAGTCC TGGCTTCGGG ATGTCTTGCG CAAGGGGCTG 
GTGAAAGCAG CTCAGAGCAC AGGTGCCTGG ATCCTGACCA GTGCCCTCCA TGTGGGCCTG 
GCACGCCATG TTGGACAGGC TGTACGTGAT CACTCTCTGG CTAGCACGTC CACCAAGGTC 
CGTGTGGTGG CCATCGGAAT GGCCTCTCTG GACCGAATCC TTCACCGCCA ACTTCTAGAT 
GGTGTCCAGG AGGATACTCC CATCCACTAC CCAGCAGATG AGGGCAGCAC TCAGGGACCC 
CTCTGCCCTC TGGACAGCAA TCTCTCCCAC TTCATCCTCG TGGAGCCAGG CACCCTTGGG 
AGTGGGAACG ACGGACTGGC AGAGCTGCAG CTGAGCCTGG AGAAGCACAT CTCTCAGCAG 
AGGACAGGTT ATGGGGGTAC CAGCAGCATC CAGATACCTG TCCTTTGCTT GCTAGTCAAT 
GGTGACCCCA GCACCCTAGA GAGGATGTCC AGGGCAGTGG AGCAGGCTGC CCCATGGCTG 
ATCCTGGCAG GTTCTGGGGG CATTGCTGAT GTACTCGCTG CCCTGGTGGG CCAGCCTCAT 
CTCCTGGTGC CCCAGGTGAC CGAGAAGCAG TTCAGAGAGA AATTCCCAAG CGAGTGTTTC 
TCTTGGGAAG CCATTGTACA CTGGACAGAG CTGCTACAGA ACATTGCTGC ACACCCCCAC 
CTGCTCACAG TGTACGACTT TGAGCAGGAG GGTTCCGAGG ACCTGGACAC CGTCATCCTC 
AAGGCACTTG TGAAAGCCTG CAAGAGTCAC AGCCGAGACG CACAAGACTA CCTAGATGAG 
CTCAAGTTAG CAGTGGCCTG GGATCGCGTG GACATTGCCA AGAGTGAAAT CTTCAATGGG 
GACGTGGAGT GGAAGTCCTG TGACTTGGAA GAGGTGATGA CAGATGCCCT AGTGAGCAAC 
AAGCCTGACT TCGTGCGCCT CTTTGTGGAC AGTGGTGCTG ACATGGCCGA GTTCTTGACC 
TATGGGCGGC TGCAGCAGCT TTACCACTCT GTGTCCCCCA AGAGCCTCCT CTTTGAACTG 
CTGGAGCGTA AGCATGAGGA GGGTCGGCTG ACACTGGCTG GCCTGGGTGC CCAGCAGACC 
CGGGAGCTGC CCGTTGGTCT GCCTGCCTTT TCACTCCATG AGGTCTCCCG AGTTCTCAAA 
GATTTCCTGC ATGACGCCTG CCGTGGCTTC TACCAGGATG GGCGCAGGAT GGAGGAGAGA 
GGGCCACCCA AGCGGCCTGC AGGCCAGAAA TGGCTGCCGG ACCTCAGTCG GAAGAGTGAA 
GACCCATGGA GGGACCTGTT CCTTTGGGCT GTGCTGCAGA ACCGTTATGA GATGGCCACA 
TACTTCTGGG CCATGGGCCG GGAGGGTGTG GCTGCTGCTC TGGCGGCCTG CAAGATCATC 
AAGGAAATGT CCCACCTGGA GAAAGAGGCA GAGGTGGCCC GCACTATGCG TGAGGCCAAG 
TATGAGCAGC TGGCCCTCGA TCTTTTCTCA GAGTGCTACA GCAACAGTGA GGACCGTGCC 
TTTGCCCTGT TGGTGCGCAG GAACCACAGC TGGAGCAGGA CCACCTGCCT GCACCTGGCC 
ACTGAGGCCG ATGCCAAGGC CTTCTTTGCC CATGATGGTG TGCAAGCATT CCTGACGAAG 
ATCTGGTGGG GAGACATGGC CACAGGCACA CCCATCTTAC GACTTCTGGG TGCCTTCACC 
TGCCCAGCCC TCATCTACAC AAATCTCATC TCCTTCAGTG AGGATGCCCC GCAGAGGATG 
GACCTGGAAG ATCTGCAGGA GCCAGACAGT TTGGATATGG AAAAGAGCTT CCTGTGCAGC 
CATGGTGGCC AATTGGAGAA GTTAACAGAG GCGCCAAGGG CTCCTGGCGA TCTAGGCCCA 
CAAGCTGCCT TCCTGCTCAC ACGGTGGAGG AAGTTCTGGG GCGCTCCTGT GACTGTGTTC 
TTGGGGAATG TGGTCATGTA CTTTGCATTC CTCTTCCTAT TCTCCTACGT CCTGCTGGTG 
GATTTCAGGC CACCACCCCA GGGGCCATCT GGGTCGGAAG TTACCCTGTA TTTCTGGGTC 
TTCACACTGG TGCTGGAGGA AATCCGACAG GGATTCTTCA CAAACGAGGA CACCCGTCTG 
GTGAAGAAGT TCACTCTGTA CGTAGAAGAC AACTGGAACA AATGTGACAT GGTGGCCATC 
TTCCTGTTCA TTGTTGGTGT CACCTGTAGA ATGGTGCCCT CCGTGTTTGA GGCTGGCCGG 
ACTGTTCTGG CCATTGACTT CATGGTGTTC ACACTTCGGC TCATCCACAT CTTTGCTATT 
CACAAGCAGC TGGGTCCTAA GATCATCATT GTAGAGCGGA TGATGAAAGA TGTCTTCTTC 
TTCCTCTTCT TCCTGAGCGT GTGGCTCGTG GCCTATGGCG TGACCACTCA GGCCCTGCTG 
CACCCCCACG ATGGCCGTCT GGAGTGGATT TTCCGCCGTG TGCTCTACAG GCCTTACCTG 
CAGATCTTTG GGCAAATCCC TCTGGATGAA ATTGATGAGG CCCGTGTGAA CTGCTCTCTT 
CACCCGTTGC TGCTGGACAG CTCAGCTTCC TGCCCTAATC TCTATGCCAA CTGGCTGGTC 
ATTCTCCTGC TGGTTACCTT CCTCCTCGTC ACTAATGTGC TACTTATGAA CCTTCTGATC 
GCCATGTTCA GCTACACATT CCAGGTGGTG CAGGGCAATG CAGACATGTT CTGGAAGTTT 
CAACGCTACC ACCTCATCGT TGAATACCAC GGAAGGCCGG CTCTGGCCCC GCCCTTCATC 
CTGCTCAGCC ACCTGAGCCT GGTGCTCAAG CAGGTCTTCA GGAAGGAAGC CCAGCACAAA 
CAGCAACACC TGGAGAGAGA CTTGCCTGAC CCCGTGGACC AGAAGATCAT TACCTGGGAA 
ACAGTTCAAA AGGAGAACTT CCTGAGTACC ATGGAGAAAC GGAGGAGGGA CAGTGAGGAG 
GAGGTGCTGA GGAAAACGGC ACACAGAGTG GACTTGATTG CCAAATACAT CGGGGGTCTG 
AGAGAGCAAG AAAAGAGGAT CAAGTGTCTG GAGTCACAGG CCAACTACTG TATGCTCCTC 
TTGTCCTCCA TGACTGACAC ACTGGCTCCT GGAGGCACCT ACTCAAGTTC TCAAAACTGT 
GGTCGCAGGA GTCAGCCAGC CTCTGCTAGA GACAGGGAGT ACCTAGAGGC TGGCTTGCCA 
CACTCAGACA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444991.1
CDS	93..3587
Translation	MPGVRKVTMPMAQSSCPGSPPDTGDGWEPVLCKGEVNFGGSGKKRSKFVKVPSNVAPSMLFELLLTEWHLPAPNLVVSLVGEERLFAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKVRVVAIGMASLDRILHRQLLDGVQEDTPIHYPADEGSTQGPLCPLDSNLSHFILVEPGTLGSGNDGLAELQLSLEKHISQQRTGYGGTSSIQIPVLCLLVNGDPSTLERMSRAVEQAAPWLILAGSGGIADVLAALVGQPHLLVPQVTEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSRDAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLERKHEEGRLTLAGLGAQQTRELPVGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALDLFSECYSNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSHGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFSYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTNEDTRLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLDSSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKQQHLERDLPDPVDQKIITWETVQKENFLSTMEKRRRDSEEEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGRRSQPASARDREYLEAGLPHSDT

Target ORF information:

RefSeq Version	XM_017589502.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589502.1

ORF Insert Sequence:

ATGCCCGGAG TTCGAAAAGT CACAATGCCG ATGGCCCAGA GCTCTTGTCC TGGAAGCCCC 
CCAGATACTG GGGATGGATG GGAGCCAGTC CTATGCAAGG GAGAGGTCAA CTTCGGAGGG 
TCTGGGAAAA AGCGAAGCAA GTTTGTGAAG GTGCCAAGCA ATGTGGCCCC CTCCATGCTC 
TTTGAACTCC TGCTCACCGA GTGGCACCTG CCAGCCCCCA ACCTGGTGGT GTCCCTGGTG 
GGCGAGGAAC GGCTTTTTGC TATGAAGTCC TGGCTTCGGG ATGTCTTGCG CAAGGGGCTG 
GTGAAAGCAG CTCAGAGCAC AGGTGCCTGG ATCCTGACCA GTGCCCTCCA TGTGGGCCTG 
GCACGCCATG TTGGACAGGC TGTACGTGAT CACTCTCTGG CTAGCACGTC CACCAAGGTC 
CGTGTGGTGG CCATCGGAAT GGCCTCTCTG GACCGAATCC TTCACCGCCA ACTTCTAGAT 
GGTGTCCAGG AGGATACTCC CATCCACTAC CCAGCAGATG AGGGCAGCAC TCAGGGACCC 
CTCTGCCCTC TGGACAGCAA TCTCTCCCAC TTCATCCTCG TGGAGCCAGG CACCCTTGGG 
AGTGGGAACG ACGGACTGGC AGAGCTGCAG CTGAGCCTGG AGAAGCACAT CTCTCAGCAG 
AGGACAGGTT ATGGGGGTAC CAGCAGCATC CAGATACCTG TCCTTTGCTT GCTAGTCAAT 
GGTGACCCCA GCACCCTAGA GAGGATGTCC AGGGCAGTGG AGCAGGCTGC CCCATGGCTG 
ATCCTGGCAG GTTCTGGGGG CATTGCTGAT GTACTCGCTG CCCTGGTGGG CCAGCCTCAT 
CTCCTGGTGC CCCAGGTGAC CGAGAAGCAG TTCAGAGAGA AATTCCCAAG CGAGTGTTTC 
TCTTGGGAAG CCATTGTACA CTGGACAGAG CTGCTACAGA ACATTGCTGC ACACCCCCAC 
CTGCTCACAG TGTACGACTT TGAGCAGGAG GGTTCCGAGG ACCTGGACAC CGTCATCCTC 
AAGGCACTTG TGAAAGCCTG CAAGAGTCAC AGCCGAGACG CACAAGACTA CCTAGATGAG 
CTCAAGTTAG CAGTGGCCTG GGATCGCGTG GACATTGCCA AGAGTGAAAT CTTCAATGGG 
GACGTGGAGT GGAAGTCCTG TGACTTGGAA GAGGTGATGA CAGATGCCCT AGTGAGCAAC 
AAGCCTGACT TCGTGCGCCT CTTTGTGGAC AGTGGTGCTG ACATGGCCGA GTTCTTGACC 
TATGGGCGGC TGCAGCAGCT TTACCACTCT GTGTCCCCCA AGAGCCTCCT CTTTGAACTG 
CTGGAGCGTA AGCATGAGGA GGGTCGGCTG ACACTGGCTG GCCTGGGTGC CCAGCAGACC 
CGGGAGCTGC CCGTTGGTCT GCCTGCCTTT TCACTCCATG AGGTCTCCCG AGTTCTCAAA 
GATTTCCTGC ATGACGCCTG CCGTGGCTTC TACCAGGATG GGCGCAGGAT GGAGGAGAGA 
GGGCCACCCA AGCGGCCTGC AGGCCAGAAA TGGCTGCCGG ACCTCAGTCG GAAGAGTGAA 
GACCCATGGA GGGACCTGTT CCTTTGGGCT GTGCTGCAGA ACCGTTATGA GATGGCCACA 
TACTTCTGGG CCATGGGCCG GGAGGGTGTG GCTGCTGCTC TGGCGGCCTG CAAGATCATC 
AAGGAAATGT CCCACCTGGA GAAAGAGGCA GAGGTGGCCC GCACTATGCG TGAGGCCAAG 
TATGAGCAGC TGGCCCTCGA TCTTTTCTCA GAGTGCTACA GCAACAGTGA GGACCGTGCC 
TTTGCCCTGT TGGTGCGCAG GAACCACAGC TGGAGCAGGA CCACCTGCCT GCACCTGGCC 
ACTGAGGCCG ATGCCAAGGC CTTCTTTGCC CATGATGGTG TGCAAGCATT CCTGACGAAG 
ATCTGGTGGG GAGACATGGC CACAGGCACA CCCATCTTAC GACTTCTGGG TGCCTTCACC 
TGCCCAGCCC TCATCTACAC AAATCTCATC TCCTTCAGTG AGGATGCCCC GCAGAGGATG 
GACCTGGAAG ATCTGCAGGA GCCAGACAGT TTGGATATGG AAAAGAGCTT CCTGTGCAGC 
CATGGTGGCC AATTGGAGAA GTTAACAGAG GCGCCAAGGG CTCCTGGCGA TCTAGGCCCA 
CAAGCTGCCT TCCTGCTCAC ACGGTGGAGG AAGTTCTGGG GCGCTCCTGT GACTGTGTTC 
TTGGGGAATG TGGTCATGTA CTTTGCATTC CTCTTCCTAT TCTCCTACGT CCTGCTGGTG 
GATTTCAGGC CACCACCCCA GGGGCCATCT GGGTCGGAAG TTACCCTGTA TTTCTGGGTC 
TTCACACTGG TGCTGGAGGA AATCCGACAG GGATTCTTCA CAAACGAGGA CACCCGTCTG 
GTGAAGAAGT TCACTCTGTA CGTAGAAGAC AACTGGAACA AATGTGACAT GGTGGCCATC 
TTCCTGTTCA TTGTTGGTGT CACCTGTAGA ATGGTGCCCT CCGTGTTTGA GGCTGGCCGG 
ACTGTTCTGG CCATTGACTT CATGGTGTTC ACACTTCGGC TCATCCACAT CTTTGCTATT 
CACAAGCAGC TGGGTCCTAA GATCATCATT GTAGAGCGGA TGATGAAAGA TGTCTTCTTC 
TTCCTCTTCT TCCTGAGCGT GTGGCTCGTG GCCTATGGCG TGACCACTCA GGCCCTGCTG 
CACCCCCACG ATGGCCGTCT GGAGTGGATT TTCCGCCGTG TGCTCTACAG GCCTTACCTG 
CAGATCTTTG GGCAAATCCC TCTGGATGAA ATTGATGAGG CCCGTGTGAA CTGCTCTCTT 
CACCCGTTGC TGCTGGACAG CTCAGCTTCC TGCCCTAATC TCTATGCCAA CTGGCTGGTC 
ATTCTCCTGC TGGTTACCTT CCTCCTCGTC ACTAATGTGC TACTTATGAA CCTTCTGATC 
GCCATGTTCA GCTACACATT CCAGGTGGTG CAGGGCAATG CAGACATGTT CTGGAAGTTT 
CAACGCTACC ACCTCATCGT TGAATACCAC GGAAGGCCGG CTCTGGCCCC GCCCTTCATC 
CTGCTCAGCC ACCTGAGCCT GGTGCTCAAG CAGGTCTTCA GGAAGGAAGC CCAGCACAAA 
CAGCAACACC TGGAGAGAGA CTTGCCTGAC CCCGTGGACC AGAAGATCAT TACCTGGGAA 
ACAGTTCAAA AGGAGAACTT CCTGAGTACC ATGGAGAAAC GGAGGAGGGA CAGTGAGGAG 
GAGGTGCTGA GGAAAACGGC ACACAGAGTG GACTTGATTG CCAAATACAT CGGGGGTCTG 
AGAGAGCAAG AAAAGAGGAT CAAGTGTCTG GAGTCACAGG CCAACTACTG TATGCTCCTC 
TTGTCCTCCA TGACTGACAC ACTGGCTCCT GGAGGCACCT ACTCAAGTTC TCAAAACTGT 
GGTCGCAGGA GTCAGCCAGC CTCTGCTAGA GACAGGGAGT ACCTAGAGGC TGGCTTGCCA 
CACTCAGACA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trpm5 ( XM_008760160.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758382.1 Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

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CloneID	ORa48727
Clone ID Related Accession (Same CDS sequence)	XM_008760160.2
Accession Version	XM_008760160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transient receptor potential cation channel subfamily M member 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG TCTGGAAGGA ACAGACAGGA GACCCTCTGC AAAAGCCTCT GGAGAGCTGT 
GTCGAGGGTG TTGGAATCCA GATGCCCGGA GTTCGAAAAG TCACAATGCC GATGGCCCAG 
AGCTCTTGTC CTGGAAGCCC CCCAGATACT GGGGATGGAT GGGAGCCAGT CCTATGCAAG 
GGAGAGGTCA ACTTCGGAGG GTCTGGGAAA AAGCGAAGCA AGTTTGTGAA GGTGCCAAGC 
AATGTGGCCC CCTCCATGCT CTTTGAACTC CTGCTCACCG AGTGGCACCT GCCAGCCCCC 
AACCTGGTGG TGTCCCTGGT GGGCGAGGAA CGGCTTTTTG CTATGAAGTC CTGGCTTCGG 
GATGTCTTGC GCAAGGGGCT GGTGAAAGCA GCTCAGAGCA CAGGTGCCTG GATCCTGACC 
AGTGCCCTCC ATGTGGGCCT GGCACGCCAT GTTGGACAGG CTGTACGTGA TCACTCTCTG 
GCTAGCACGT CCACCAAGGT CCGTGTGGTG GCCATCGGAA TGGCCTCTCT GGACCGAATC 
CTTCACCGCC AACTTCTAGA TGGTGTCCAG GAGGATACTC CCATCCACTA CCCAGCAGAT 
GAGGGCAGCA CTCAGGGACC CCTCTGCCCT CTGGACAGCA ATCTCTCCCA CTTCATCCTC 
GTGGAGCCAG GCACCCTTGG GAGTGGGAAC GACGGACTGG CAGAGCTGCA GCTGAGCCTG 
GAGAAGCACA TCTCTCAGCA GAGGACAGGT TATGGGGGTA CCAGCAGCAT CCAGATACCT 
GTCCTTTGCT TGCTAGTCAA TGGTGACCCC AGCACCCTAG AGAGGATGTC CAGGGCAGTG 
GAGCAGGCTG CCCCATGGCT GATCCTGGCA GGTTCTGGGG GCATTGCTGA TGTACTCGCT 
GCCCTGGTGG GCCAGCCTCA TCTCCTGGTG CCCCAGGTGA CCGAGAAGCA GTTCAGAGAG 
AAATTCCCAA GCGAGTGTTT CTCTTGGGAA GCCATTGTAC ACTGGACAGA GCTGCTACAG 
AACATTGCTG CACACCCCCA CCTGCTCACA GTGTACGACT TTGAGCAGGA GGGTTCCGAG 
GACCTGGACA CCGTCATCCT CAAGGCACTT GTGAAAGCCT GCAAGAGTCA CAGCCGAGAC 
GCACAAGACT ACCTAGATGA GCTCAAGTTA GCAGTGGCCT GGGATCGCGT GGACATTGCC 
AAGAGTGAAA TCTTCAATGG GGACGTGGAG TGGAAGTCCT GTGACTTGGA AGAGGTGATG 
ACAGATGCCC TAGTGAGCAA CAAGCCTGAC TTCGTGCGCC TCTTTGTGGA CAGTGGTGCT 
GACATGGCCG AGTTCTTGAC CTATGGGCGG CTGCAGCAGC TTTACCACTC TGTGTCCCCC 
AAGAGCCTCC TCTTTGAACT GCTGGAGCGT AAGCATGAGG AGGGTCGGCT GACACTGGCT 
GGCCTGGGTG CCCAGCAGAC CCGGGAGCTG CCCGTTGGTC TGCCTGCCTT TTCACTCCAT 
GAGGTCTCCC GAGTTCTCAA AGATTTCCTG CATGACGCCT GCCGTGGCTT CTACCAGGAT 
GGGCGCAGGA TGGAGGAGAG AGGGCCACCC AAGCGGCCTG CAGGCCAGAA ATGGCTGCCG 
GACCTCAGTC GGAAGAGTGA AGACCCATGG AGGGACCTGT TCCTTTGGGC TGTGCTGCAG 
AACCGTTATG AGATGGCCAC ATACTTCTGG GCCATGGGCC GGGAGGGTGT GGCTGCTGCT 
CTGGCGGCCT GCAAGATCAT CAAGGAAATG TCCCACCTGG AGAAAGAGGC AGAGGTGGCC 
CGCACTATGC GTGAGGCCAA GTATGAGCAG CTGGCCCTCG AGTGCTACAG CAACAGTGAG 
GACCGTGCCT TTGCCCTGTT GGTGCGCAGG AACCACAGCT GGAGCAGGAC CACCTGCCTG 
CACCTGGCCA CTGAGGCCGA TGCCAAGGCC TTCTTTGCCC ATGATGGTGT GCAAGCATTC 
CTGACGAAGA TCTGGTGGGG AGACATGGCC ACAGGCACAC CCATCTTACG ACTTCTGGGT 
GCCTTCACCT GCCCAGCCCT CATCTACACA AATCTCATCT CCTTCAGTGA GGATGCCCCG 
CAGAGGATGG ACCTGGAAGA TCTGCAGGAG CCAGACAGTT TGGATATGGA AAAGAGCTTC 
CTGTGCAGCC ATGGTGGCCA ATTGGAGAAG TTAACAGAGG CGCCAAGGGC TCCTGGCGAT 
CTAGGCCCAC AAGCTGCCTT CCTGCTCACA CGGTGGAGGA AGTTCTGGGG CGCTCCTGTG 
ACTGTGTTCT TGGGGAATGT GGTCATGTAC TTTGCATTCC TCTTCCTATT CTCCTACGTC 
CTGCTGGTGG ATTTCAGGCC ACCACCCCAG GGGCCATCTG GGTCGGAAGT TACCCTGTAT 
TTCTGGGTCT TCACACTGGT GCTGGAGGAA ATCCGACAGG GATTCTTCAC AAACGAGGAC 
ACCCGTCTGG TGAAGAAGTT CACTCTGTAC GTAGAAGACA ACTGGAACAA ATGTGACATG 
GTGGCCATCT TCCTGTTCAT TGTTGGTGTC ACCTGTAGAA TGGTGCCCTC CGTGTTTGAG 
GCTGGCCGGA CTGTTCTGGC CATTGACTTC ATGGTGTTCA CACTTCGGCT CATCCACATC 
TTTGCTATTC ACAAGCAGCT GGGTCCTAAG ATCATCATTG TAGAGCGGAT GATGAAAGAT 
GTCTTCTTCT TCCTCTTCTT CCTGAGCGTG TGGCTCGTGG CCTATGGCGT GACCACTCAG 
GCCCTGCTGC ACCCCCACGA TGGCCGTCTG GAGTGGATTT TCCGCCGTGT GCTCTACAGG 
CCTTACCTGC AGATCTTTGG GCAAATCCCT CTGGATGAAA TTGATGAGGC CCGTGTGAAC 
TGCTCTCTTC ACCCGTTGCT GCTGGACAGC TCAGCTTCCT GCCCTAATCT CTATGCCAAC 
TGGCTGGTCA TTCTCCTGCT GGTTACCTTC CTCCTCGTCA CTAATGTGCT ACTTATGAAC 
CTTCTGATCG CCATGTTCAG CTACACATTC CAGGTGGTGC AGGGCAATGC AGACATGTTC 
TGGAAGTTTC AACGCTACCA CCTCATCGTT GAATACCACG GAAGGCCGGC TCTGGCCCCG 
CCCTTCATCC TGCTCAGCCA CCTGAGCCTG GTGCTCAAGC AGGTCTTCAG GAAGGAAGCC 
CAGCACAAAC AGCAACACCT GGAGAGAGAC TTGCCTGACC CCGTGGACCA GAAGATCATT 
ACCTGGGAAA CAGTTCAAAA GGAGAACTTC CTGAGTACCA TGGAGAAACG GAGGAGGGAC 
AGTGAGGAGG AGGTGCTGAG GAAAACGGCA CACAGAGTGG ACTTGATTGC CAAATACATC 
GGGGGTCTGA GAGAGCAAGA AAAGAGGATC AAGTGTCTGG AGTCACAGGC CAACTACTGT 
ATGCTCCTCT TGTCCTCCAT GACTGACACA CTGGCTCCTG GAGGCACCTA CTCAAGTTCT 
CAAAACTGTG GTCGCAGGAG TCAGCCAGCC TCTGCTAGAG ACAGGGAGTA CCTAGAGGCT 
GGCTTGCCAC ACTCAGACAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758382.1
CDS	2107..5670
Translation	MPGVWKEQTGDPLQKPLESCVEGVGIQMPGVRKVTMPMAQSSCPGSPPDTGDGWEPVLCKGEVNFGGSGKKRSKFVKVPSNVAPSMLFELLLTEWHLPAPNLVVSLVGEERLFAMKSWLRDVLRKGLVKAAQSTGAWILTSALHVGLARHVGQAVRDHSLASTSTKVRVVAIGMASLDRILHRQLLDGVQEDTPIHYPADEGSTQGPLCPLDSNLSHFILVEPGTLGSGNDGLAELQLSLEKHISQQRTGYGGTSSIQIPVLCLLVNGDPSTLERMSRAVEQAAPWLILAGSGGIADVLAALVGQPHLLVPQVTEKQFREKFPSECFSWEAIVHWTELLQNIAAHPHLLTVYDFEQEGSEDLDTVILKALVKACKSHSRDAQDYLDELKLAVAWDRVDIAKSEIFNGDVEWKSCDLEEVMTDALVSNKPDFVRLFVDSGADMAEFLTYGRLQQLYHSVSPKSLLFELLERKHEEGRLTLAGLGAQQTRELPVGLPAFSLHEVSRVLKDFLHDACRGFYQDGRRMEERGPPKRPAGQKWLPDLSRKSEDPWRDLFLWAVLQNRYEMATYFWAMGREGVAAALAACKIIKEMSHLEKEAEVARTMREAKYEQLALECYSNSEDRAFALLVRRNHSWSRTTCLHLATEADAKAFFAHDGVQAFLTKIWWGDMATGTPILRLLGAFTCPALIYTNLISFSEDAPQRMDLEDLQEPDSLDMEKSFLCSHGGQLEKLTEAPRAPGDLGPQAAFLLTRWRKFWGAPVTVFLGNVVMYFAFLFLFSYVLLVDFRPPPQGPSGSEVTLYFWVFTLVLEEIRQGFFTNEDTRLVKKFTLYVEDNWNKCDMVAIFLFIVGVTCRMVPSVFEAGRTVLAIDFMVFTLRLIHIFAIHKQLGPKIIIVERMMKDVFFFLFFLSVWLVAYGVTTQALLHPHDGRLEWIFRRVLYRPYLQIFGQIPLDEIDEARVNCSLHPLLLDSSASCPNLYANWLVILLLVTFLLVTNVLLMNLLIAMFSYTFQVVQGNADMFWKFQRYHLIVEYHGRPALAPPFILLSHLSLVLKQVFRKEAQHKQQHLERDLPDPVDQKIITWETVQKENFLSTMEKRRRDSEEEVLRKTAHRVDLIAKYIGGLREQEKRIKCLESQANYCMLLLSSMTDTLAPGGTYSSSQNCGRRSQPASARDREYLEAGLPHSDT

Target ORF information:

RefSeq Version	XM_008760160.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transient receptor potential cation channel, subfamily M, member 5 (Trpm5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760160.2

ORF Insert Sequence:

ATGCCAGGAG TCTGGAAGGA ACAGACAGGA GACCCTCTGC AAAAGCCTCT GGAGAGCTGT 
GTCGAGGGTG TTGGAATCCA GATGCCCGGA GTTCGAAAAG TCACAATGCC GATGGCCCAG 
AGCTCTTGTC CTGGAAGCCC CCCAGATACT GGGGATGGAT GGGAGCCAGT CCTATGCAAG 
GGAGAGGTCA ACTTCGGAGG GTCTGGGAAA AAGCGAAGCA AGTTTGTGAA GGTGCCAAGC 
AATGTGGCCC CCTCCATGCT CTTTGAACTC CTGCTCACCG AGTGGCACCT GCCAGCCCCC 
AACCTGGTGG TGTCCCTGGT GGGCGAGGAA CGGCTTTTTG CTATGAAGTC CTGGCTTCGG 
GATGTCTTGC GCAAGGGGCT GGTGAAAGCA GCTCAGAGCA CAGGTGCCTG GATCCTGACC 
AGTGCCCTCC ATGTGGGCCT GGCACGCCAT GTTGGACAGG CTGTACGTGA TCACTCTCTG 
GCTAGCACGT CCACCAAGGT CCGTGTGGTG GCCATCGGAA TGGCCTCTCT GGACCGAATC 
CTTCACCGCC AACTTCTAGA TGGTGTCCAG GAGGATACTC CCATCCACTA CCCAGCAGAT 
GAGGGCAGCA CTCAGGGACC CCTCTGCCCT CTGGACAGCA ATCTCTCCCA CTTCATCCTC 
GTGGAGCCAG GCACCCTTGG GAGTGGGAAC GACGGACTGG CAGAGCTGCA GCTGAGCCTG 
GAGAAGCACA TCTCTCAGCA GAGGACAGGT TATGGGGGTA CCAGCAGCAT CCAGATACCT 
GTCCTTTGCT TGCTAGTCAA TGGTGACCCC AGCACCCTAG AGAGGATGTC CAGGGCAGTG 
GAGCAGGCTG CCCCATGGCT GATCCTGGCA GGTTCTGGGG GCATTGCTGA TGTACTCGCT 
GCCCTGGTGG GCCAGCCTCA TCTCCTGGTG CCCCAGGTGA CCGAGAAGCA GTTCAGAGAG 
AAATTCCCAA GCGAGTGTTT CTCTTGGGAA GCCATTGTAC ACTGGACAGA GCTGCTACAG 
AACATTGCTG CACACCCCCA CCTGCTCACA GTGTACGACT TTGAGCAGGA GGGTTCCGAG 
GACCTGGACA CCGTCATCCT CAAGGCACTT GTGAAAGCCT GCAAGAGTCA CAGCCGAGAC 
GCACAAGACT ACCTAGATGA GCTCAAGTTA GCAGTGGCCT GGGATCGCGT GGACATTGCC 
AAGAGTGAAA TCTTCAATGG GGACGTGGAG TGGAAGTCCT GTGACTTGGA AGAGGTGATG 
ACAGATGCCC TAGTGAGCAA CAAGCCTGAC TTCGTGCGCC TCTTTGTGGA CAGTGGTGCT 
GACATGGCCG AGTTCTTGAC CTATGGGCGG CTGCAGCAGC TTTACCACTC TGTGTCCCCC 
AAGAGCCTCC TCTTTGAACT GCTGGAGCGT AAGCATGAGG AGGGTCGGCT GACACTGGCT 
GGCCTGGGTG CCCAGCAGAC CCGGGAGCTG CCCGTTGGTC TGCCTGCCTT TTCACTCCAT 
GAGGTCTCCC GAGTTCTCAA AGATTTCCTG CATGACGCCT GCCGTGGCTT CTACCAGGAT 
GGGCGCAGGA TGGAGGAGAG AGGGCCACCC AAGCGGCCTG CAGGCCAGAA ATGGCTGCCG 
GACCTCAGTC GGAAGAGTGA AGACCCATGG AGGGACCTGT TCCTTTGGGC TGTGCTGCAG 
AACCGTTATG AGATGGCCAC ATACTTCTGG GCCATGGGCC GGGAGGGTGT GGCTGCTGCT 
CTGGCGGCCT GCAAGATCAT CAAGGAAATG TCCCACCTGG AGAAAGAGGC AGAGGTGGCC 
CGCACTATGC GTGAGGCCAA GTATGAGCAG CTGGCCCTCG AGTGCTACAG CAACAGTGAG 
GACCGTGCCT TTGCCCTGTT GGTGCGCAGG AACCACAGCT GGAGCAGGAC CACCTGCCTG 
CACCTGGCCA CTGAGGCCGA TGCCAAGGCC TTCTTTGCCC ATGATGGTGT GCAAGCATTC 
CTGACGAAGA TCTGGTGGGG AGACATGGCC ACAGGCACAC CCATCTTACG ACTTCTGGGT 
GCCTTCACCT GCCCAGCCCT CATCTACACA AATCTCATCT CCTTCAGTGA GGATGCCCCG 
CAGAGGATGG ACCTGGAAGA TCTGCAGGAG CCAGACAGTT TGGATATGGA AAAGAGCTTC 
CTGTGCAGCC ATGGTGGCCA ATTGGAGAAG TTAACAGAGG CGCCAAGGGC TCCTGGCGAT 
CTAGGCCCAC AAGCTGCCTT CCTGCTCACA CGGTGGAGGA AGTTCTGGGG CGCTCCTGTG 
ACTGTGTTCT TGGGGAATGT GGTCATGTAC TTTGCATTCC TCTTCCTATT CTCCTACGTC 
CTGCTGGTGG ATTTCAGGCC ACCACCCCAG GGGCCATCTG GGTCGGAAGT TACCCTGTAT 
TTCTGGGTCT TCACACTGGT GCTGGAGGAA ATCCGACAGG GATTCTTCAC AAACGAGGAC 
ACCCGTCTGG TGAAGAAGTT CACTCTGTAC GTAGAAGACA ACTGGAACAA ATGTGACATG 
GTGGCCATCT TCCTGTTCAT TGTTGGTGTC ACCTGTAGAA TGGTGCCCTC CGTGTTTGAG 
GCTGGCCGGA CTGTTCTGGC CATTGACTTC ATGGTGTTCA CACTTCGGCT CATCCACATC 
TTTGCTATTC ACAAGCAGCT GGGTCCTAAG ATCATCATTG TAGAGCGGAT GATGAAAGAT 
GTCTTCTTCT TCCTCTTCTT CCTGAGCGTG TGGCTCGTGG CCTATGGCGT GACCACTCAG 
GCCCTGCTGC ACCCCCACGA TGGCCGTCTG GAGTGGATTT TCCGCCGTGT GCTCTACAGG 
CCTTACCTGC AGATCTTTGG GCAAATCCCT CTGGATGAAA TTGATGAGGC CCGTGTGAAC 
TGCTCTCTTC ACCCGTTGCT GCTGGACAGC TCAGCTTCCT GCCCTAATCT CTATGCCAAC 
TGGCTGGTCA TTCTCCTGCT GGTTACCTTC CTCCTCGTCA CTAATGTGCT ACTTATGAAC 
CTTCTGATCG CCATGTTCAG CTACACATTC CAGGTGGTGC AGGGCAATGC AGACATGTTC 
TGGAAGTTTC AACGCTACCA CCTCATCGTT GAATACCACG GAAGGCCGGC TCTGGCCCCG 
CCCTTCATCC TGCTCAGCCA CCTGAGCCTG GTGCTCAAGC AGGTCTTCAG GAAGGAAGCC 
CAGCACAAAC AGCAACACCT GGAGAGAGAC TTGCCTGACC CCGTGGACCA GAAGATCATT 
ACCTGGGAAA CAGTTCAAAA GGAGAACTTC CTGAGTACCA TGGAGAAACG GAGGAGGGAC 
AGTGAGGAGG AGGTGCTGAG GAAAACGGCA CACAGAGTGG ACTTGATTGC CAAATACATC 
GGGGGTCTGA GAGAGCAAGA AAAGAGGATC AAGTGTCTGG AGTCACAGGC CAACTACTGT 
ATGCTCCTCT TGTCCTCCAT GACTGACACA CTGGCTCCTG GAGGCACCTA CTCAAGTTCT 
CAAAACTGTG GTCGCAGGAG TCAGCCAGCC TCTGCTAGAG ACAGGGAGTA CCTAGAGGCT 
GGCTTGCCAC ACTCAGACAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs).
PloS one13(1)e0190465(2018)
Qian J,Mummalaneni S,Grider JR,Damaj MI,Lyall V

Expression of sweet taste receptor and gut hormone secretion in modelled type 2 diabetes.
General and comparative endocrinology252142-149(2017 Oct)
Feng R,Qian C,Liu Q,Jin Y,Liu L,Li S,Liao Y,Zhou H,Liu W,Rayner CK,Ma J

Role of transient receptor potential melastatin-like subtype 5 channel in insulin secretion from rat ?-cells.
Pancreas43(4)597-604(2014 May)
Krishnan K,Ma Z,Bj?rklund A,Islam MS

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response.
American journal of physiology. Gastrointestinal and liver physiology305(1)G106-17(2013 Jul)
Ren Z,Rhyu MR,Phan TH,Mummalaneni S,Murthy KS,Grider JR,DeSimone JA,Lyall V

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Trpm5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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