Rnf31 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf31
Entrez Gene ID	364386
Full Name	ring finger protein 31
General protein information	Preferred Names ring finger protein 31 Names E3 ubiquitin-protein ligase RNF31
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13

mRNA and Protein(s)

mRNA	Protein	Name
XM_006252000.3	XP_006252062.1	E3 ubiquitin-protein ligase RNF31 isoform X1
NM_001108868.1	NP_001102338.2	E3 ubiquitin-protein ligase RNF31

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF31	NP_060469.4
Pan troglodytes (chimpanzee)	RNF31	XP_001166671.1
Canis lupus familiaris (dog)	RNF31	XP_537383.2
Xenopus tropicalis (tropical clawed frog)	rnf31	NP_001090644.1
	RNF31	XP_001112195.1
Mus musculus (house mouse)	Rnf31	NP_919327.2
Rattus norvegicus (Norway rat)	Rnf31	NP_001102338.2
Danio rerio (zebrafish)	LOC100334103	XP_002660873.3

Gene Ontology
Bibliography

Related articles in PubMed

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Damgaard RB, Walker JA, Marco-Casanova P, Morgan NV, Titheradge HL, Elliott PR, McHale D, Maher ER, McKenzie ANJ, Komander D
Cell166(5)1215-1230.e20(2016 Aug)

Molecular basis and regulation of OTULIN-LUBAC interaction.
Elliott PR, Nielsen SV, Marco-Casanova P, Fiil BK, Keusekotten K, Mailand N, Freund SM, Gyrd-Hansen M, Komander D
Molecular cell54(3)335-48(2014 May)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

The E3 ligase parkin maintains mitochondrial integrity by increasing linear ubiquitination of NEMO.
M?ller-Rischart AK, Pilsl A, Beaudette P, Patra M, Hadian K, Funke M, Peis R, Deinlein A, Schweimer C, Kuhn PH, Lichtenthaler SF, Motori E, Hrelia S, Wurst W, Tr?mbach D, Langer T, Krappmann D, Dittmar G, Tatzelt J, Winklhofer KF
Molecular cell49(5)908-21(2013 Mar)

Tumor necrosis factor.
Chu WM
Cancer letters328(2)222-5(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rnf31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08528	NM_001108868.1 Latest version!	Rattus norvegicus ring finger protein 31 (Rnf31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$783.30 $1119.00
ORa36694	XM_006252000.3 Latest version!	Rattus norvegicus ring finger protein 31 (Rnf31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf31 ( NM_001108868.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102338.2 Rattus norvegicus ring finger protein 31 (Rnf31), mRNA.

Price & Availability↑

CloneID	ORa08528
Clone ID Related Accession (Same CDS sequence)	NM_001108868.1
Accession Version	NM_001108868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF31
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474049.1. On or before Oct 4, 2007 this sequence version replaced XM_344409.3, XM_001059460.1, XM_001059524.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCAGGAG ACGAGGAGCG AGCCTTCCTA GCGGCCCGCG AGGAGCTGGC GAGCGCCCTG 
AGGTGGGATT CTGCGCAGGT ATTTCCCCTG GAGCAGCTCA CGCCGCTTCT GGCCACCTCT 
CTGCCACTCT CCTCCCGCTA CCGGCAGCTG GATGCCGGAC GCCTGGTCCG CTGCAACGCT 
CATGGGGAGC CTCGAAACTA CCTCAACACT CTATCCACGG CCCTGAACAT CCTGGAAAAG 
TATGGTCGAA ACCTCCTCAG CCCACAGCGG CCCCGGTATT GGCGCTCAGT GAAGTTTAAT 
AACCCTGTCT TTCGCAGCAC GGTGGATGCT GTGCAGGGTG GCCGGGATGT GCTACGGTTG 
TATGGCTATA CCGAGGAGCG CCCAGATGGA TTGAGTTTCC CCGGAGGGCA GGAGGAACCA 
GATGAATACC AGGTTGCCAT AGTCACACTA GAAGTACTAC TGCTTCGCAC GGAGCTCAAT 
TTGCTGTTGC AGAATACTCA TCCAAGACAG AACGCACTGG ACCAGCTACT AAGAGACAGC 
GTTGAAGATG ACATGCTGCA GCTTTCAGAG TTTCACCCCC TTTTGAGAGA GATTGTTCCT 
GGCCCCCTCC CCTCTGCCCC AGGCCATACT CCTGGTCCCT GCTTTCTCTG TGGTTCTGCC 
CCAGGCACAC TGCACTGTCC AGCCTGTAAC CAAGCTTCAT GCCCAGCTTG TGACCTTTTG 
TTCCATGGGC ATCCGTCCCG TGCGCATCAC CTTCGCCACA CCCTACCCGG GGCCCACCAG 
ACCACCAACC TAAGCTCTAG TTTACCTGCC TCGTCCCAAC CACGGCCCCA CTCCTCCTCC 
CTGGCCATGG GAGATAGCTC TCTCTCTTCC CCTGACCCTG CAAATGCTTG TCTGCCCTGG 
CACTGTCCTA CCTGTGCCAC ATTAAACGAG CCTTGGGCAG TGTTCTGTGC AGCCTGTAGT 
CAGCCCAAAG GCTGTAAGGT GCGGGGAAGA GAGAGTTCCC AAGGAACTGG GGTCCTAGAG 
CCCGAGCCTG CACGGGATCA GTGGGCCTGC CAGAGCTGTA CCTTTGAGAA TGAGGCAGCA 
GCCGTGCTCT GTGCCATCTG TGAGCGACCT CGGCTGGCCC AGCCTCCCAG CTTGGTGGTG 
GATTCCCATG ACGCTGGTGT TTACCAACAG CCCCTTAAGC AGGAGGATAC TTTGCTCTCC 
GCTGCCCAGT CTCAGGTGTG GCACTGTGAC CATTGTACCT TCTGCAATTC AGGCCCTGTC 
TGGGTGTGTG CCATGTGTAA CCGGACTCGC AGCCCCATCC CTGCACAGCC CTATCCCAGC 
TCCTTGGAGA AGGGACTCTC ACAGCCAGGG TCCTCACAAC ACCTCAGCTC CTCCCTGCCT 
GCTTCCTGTG GAGATCCAGA GAAACAGCGC CAAGATAAGA TGCGGAAGGA AAGTCTCCAG 
CTAGTGAACA TGATCCAGGA AGGGGAAGCT GCAGGTGCCA GTCCAGAAGA GATCTTCTCG 
GCTCTACAAT ACTCGGGCAC TGAGGTGCCC CTGCAGTGGT TGCGTTCAGA GCTGTCCTAC 
GTCTTGGAGA TGGTGGCTGA GCTAGCTGGA CAACGGGATC CAGAGCTCGG TGCCTTTTCC 
TGTCAGGAAG CCCGGAAAGC CTGGCTTGAT CGTCATGGCA ACCTTGATGA AGCTGTAGAG 
GAGTGTGTGA GGGCCAGGAG GAGGAAGGTG CAGGAGCTCC AGTCCCTGGG CTTTGAGCCT 
AAAGAAGGGT CGCTGCAGGC ATTGTTCCAG CATGGGGGTG ATGTGGCCCG GGCCCTAACT 
GAGTTACAGC GACAGCGCCT GGAGCCTTTC CACCAGCGCC TCTGGGGCAG AGACCCCGAA 
CCCACTCCCT GCTGGGATGG CCTGGATAGA CAGAGCCTGG TCAGACGCCT TCTGGCGGTA 
TACACACTCC CCAGCTGGGG TCGAGCAGAG TTGGCGTTAG CGCTGCTGCA GGAGACACCC 
AGGAACTATG AGTTGTTGGA CGTGGTGGAG GCCGTGAGGC ACAGTCAGGA CCGGGCCTTT 
CTGCGCCGAC TGCTTGCCCA GGAATGTGCT GTGTGCGGGT GGGCCCTTCC CCGAAACCGG 
ATGCAGGCCC TGATCTCCTG CGAGTGCACC ATATGCCCTG AGTGTTTCCG CCAGCATTTC 
ACCATTGCCC TGAAGGAGAA GCACATCACA GACATGGTGT GCCCTGCCTG TGGCCGCCCT 
GACCTCACCG ATGATGCGCA GTTACTCAGT TACTTCTCCA CCCTTGACAT CCAGCTCAGA 
GAGAGCCTAG AGCCGGATGC ATATGCTCTG TTTCATAAGA AGCTAACCGA GGCTGTGCTC 
ATGCGAGACC CCAAGTTCTT GTGGTGTGCC CAGTGTTCCT TCGGCTTCAT ATATGAACGA 
GAACAGCTGG AGGCGACATG TCCCCAGTGT CACCAGACCT TCTGTGTGCG CTGCAAGCGC 
CAGTGGGAGG AGCAGCACAG AGGGCGGAGC TGTGAGGATT TCCAGAACTG GAAACGCACC 
AATGACCCAG AGTACCAGGC TCAAGGCCTG GCCATGTACC TTCAGGAAAA CGGCATAGAC 
TGCCCCAAAT GCAAGTTCTC ATACGCACTG GCCCGAGGAG GCTGCATGCA CTTCCACTGC 
ACACAGTGTC GCCACCAGTT CTGCAGCGGC TGCTACAATG CCTTTTACGC CAAGAATAAA 
TGTCCAGACC CTAACTGCAA GGTGAAAAAG TCCCTGCATG GTCACCACCC TCGAGATTGC 
CTCTTCTACC TACGGGATTG GCCTGCTGCC CGGCTCCAGA AACTTTTGCA GGACAATAAT 
GTCATGTTTA ACACAGAGCC TCCTGCCGGC ACACGGGCAG TCCCTGGAGG TGGCTGCCGA 
GTGATGGAAC AGAAGGAGGT TCCCAGTGGG TTCAGGGATG AAGCTTGTGG CAAGGAAACT 
CCGCCTGGCT ATGCCGGCCT GTGTCAGGCA CACTACAAAG AGTATCTTGT GAGCCTCATC 
AATGCCCACT CGCTGGACCC AGCGACCCTG TATGAAGTGG AGGAACTGGA GACAGCCACT 
GCACGCTACC TACATTTAAC GCCCCAACCC ATGGATGGAG AGGATCTTCC TGCTTACCAG 
GCCCGGTTGT TACAGAAGCT GAGAGAAGAG GTGCCCTTGG GACAGAGTAT TGCTCGCAGA 
AGAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102338.2
CDS	119..3307
Translation	MPGDEERAFLAAREELASALRWDSAQVFPLEQLTPLLATSLPLSSRYRQLDAGRLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRSVKFNNPVFRSTVDAVQGGRDVLRLYGYTEERPDGLSFPGGQEEPDEYQVAIVTLEVLLLRTELNLLLQNTHPRQNALDQLLRDSVEDDMLQLSEFHPLLREIVPGPLPSAPGHTPGPCFLCGSAPGTLHCPACNQASCPACDLLFHGHPSRAHHLRHTLPGAHQTTNLSSSLPASSQPRPHSSSLAMGDSSLSSPDPANACLPWHCPTCATLNEPWAVFCAACSQPKGCKVRGRESSQGTGVLEPEPARDQWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSHDAGVYQQPLKQEDTLLSAAQSQVWHCDHCTFCNSGPVWVCAMCNRTRSPIPAQPYPSSLEKGLSQPGSSQHLSSSLPASCGDPEKQRQDKMRKESLQLVNMIQEGEAAGASPEEIFSALQYSGTEVPLQWLRSELSYVLEMVAELAGQRDPELGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELQSLGFEPKEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWGRDPEPTPCWDGLDRQSLVRRLLAVYTLPSWGRAELALALLQETPRNYELLDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALISCECTICPECFRQHFTIALKEKHITDMVCPACGRPDLTDDAQLLSYFSTLDIQLRESLEPDAYALFHKKLTEAVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRTNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCKVKKSLHGHHPRDCLFYLRDWPAARLQKLLQDNNVMFNTEPPAGTRAVPGGGCRVMEQKEVPSGFRDEACGKETPPGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATARYLHLTPQPMDGEDLPAYQARLLQKLREEVPLGQSIARRRK

Target ORF information:

RefSeq Version	NM_001108868.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 31 (Rnf31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108868.1

ORF Insert Sequence:

ATGCCAGGAG ACGAGGAGCG AGCCTTCCTA GCGGCCCGCG AGGAGCTGGC GAGCGCCCTG 
AGGTGGGATT CTGCGCAGGT ATTTCCCCTG GAGCAGCTCA CGCCGCTTCT GGCCACCTCT 
CTGCCACTCT CCTCCCGCTA CCGGCAGCTG GATGCCGGAC GCCTGGTCCG CTGCAACGCT 
CATGGGGAGC CTCGAAACTA CCTCAACACT CTATCCACGG CCCTGAACAT CCTGGAAAAG 
TATGGTCGAA ACCTCCTCAG CCCACAGCGG CCCCGGTATT GGCGCTCAGT GAAGTTTAAT 
AACCCTGTCT TTCGCAGCAC GGTGGATGCT GTGCAGGGTG GCCGGGATGT GCTACGGTTG 
TATGGCTATA CCGAGGAGCG CCCAGATGGA TTGAGTTTCC CCGGAGGGCA GGAGGAACCA 
GATGAATACC AGGTTGCCAT AGTCACACTA GAAGTACTAC TGCTTCGCAC GGAGCTCAAT 
TTGCTGTTGC AGAATACTCA TCCAAGACAG AACGCACTGG ACCAGCTACT AAGAGACAGC 
GTTGAAGATG ACATGCTGCA GCTTTCAGAG TTTCACCCCC TTTTGAGAGA GATTGTTCCT 
GGCCCCCTCC CCTCTGCCCC AGGCCATACT CCTGGTCCCT GCTTTCTCTG TGGTTCTGCC 
CCAGGCACAC TGCACTGTCC AGCCTGTAAC CAAGCTTCAT GCCCAGCTTG TGACCTTTTG 
TTCCATGGGC ATCCGTCCCG TGCGCATCAC CTTCGCCACA CCCTACCCGG GGCCCACCAG 
ACCACCAACC TAAGCTCTAG TTTACCTGCC TCGTCCCAAC CACGGCCCCA CTCCTCCTCC 
CTGGCCATGG GAGATAGCTC TCTCTCTTCC CCTGACCCTG CAAATGCTTG TCTGCCCTGG 
CACTGTCCTA CCTGTGCCAC ATTAAACGAG CCTTGGGCAG TGTTCTGTGC AGCCTGTAGT 
CAGCCCAAAG GCTGTAAGGT GCGGGGAAGA GAGAGTTCCC AAGGAACTGG GGTCCTAGAG 
CCCGAGCCTG CACGGGATCA GTGGGCCTGC CAGAGCTGTA CCTTTGAGAA TGAGGCAGCA 
GCCGTGCTCT GTGCCATCTG TGAGCGACCT CGGCTGGCCC AGCCTCCCAG CTTGGTGGTG 
GATTCCCATG ACGCTGGTGT TTACCAACAG CCCCTTAAGC AGGAGGATAC TTTGCTCTCC 
GCTGCCCAGT CTCAGGTGTG GCACTGTGAC CATTGTACCT TCTGCAATTC AGGCCCTGTC 
TGGGTGTGTG CCATGTGTAA CCGGACTCGC AGCCCCATCC CTGCACAGCC CTATCCCAGC 
TCCTTGGAGA AGGGACTCTC ACAGCCAGGG TCCTCACAAC ACCTCAGCTC CTCCCTGCCT 
GCTTCCTGTG GAGATCCAGA GAAACAGCGC CAAGATAAGA TGCGGAAGGA AAGTCTCCAG 
CTAGTGAACA TGATCCAGGA AGGGGAAGCT GCAGGTGCCA GTCCAGAAGA GATCTTCTCG 
GCTCTACAAT ACTCGGGCAC TGAGGTGCCC CTGCAGTGGT TGCGTTCAGA GCTGTCCTAC 
GTCTTGGAGA TGGTGGCTGA GCTAGCTGGA CAACGGGATC CAGAGCTCGG TGCCTTTTCC 
TGTCAGGAAG CCCGGAAAGC CTGGCTTGAT CGTCATGGCA ACCTTGATGA AGCTGTAGAG 
GAGTGTGTGA GGGCCAGGAG GAGGAAGGTG CAGGAGCTCC AGTCCCTGGG CTTTGAGCCT 
AAAGAAGGGT CGCTGCAGGC ATTGTTCCAG CATGGGGGTG ATGTGGCCCG GGCCCTAACT 
GAGTTACAGC GACAGCGCCT GGAGCCTTTC CACCAGCGCC TCTGGGGCAG AGACCCCGAA 
CCCACTCCCT GCTGGGATGG CCTGGATAGA CAGAGCCTGG TCAGACGCCT TCTGGCGGTA 
TACACACTCC CCAGCTGGGG TCGAGCAGAG TTGGCGTTAG CGCTGCTGCA GGAGACACCC 
AGGAACTATG AGTTGTTGGA CGTGGTGGAG GCCGTGAGGC ACAGTCAGGA CCGGGCCTTT 
CTGCGCCGAC TGCTTGCCCA GGAATGTGCT GTGTGCGGGT GGGCCCTTCC CCGAAACCGG 
ATGCAGGCCC TGATCTCCTG CGAGTGCACC ATATGCCCTG AGTGTTTCCG CCAGCATTTC 
ACCATTGCCC TGAAGGAGAA GCACATCACA GACATGGTGT GCCCTGCCTG TGGCCGCCCT 
GACCTCACCG ATGATGCGCA GTTACTCAGT TACTTCTCCA CCCTTGACAT CCAGCTCAGA 
GAGAGCCTAG AGCCGGATGC ATATGCTCTG TTTCATAAGA AGCTAACCGA GGCTGTGCTC 
ATGCGAGACC CCAAGTTCTT GTGGTGTGCC CAGTGTTCCT TCGGCTTCAT ATATGAACGA 
GAACAGCTGG AGGCGACATG TCCCCAGTGT CACCAGACCT TCTGTGTGCG CTGCAAGCGC 
CAGTGGGAGG AGCAGCACAG AGGGCGGAGC TGTGAGGATT TCCAGAACTG GAAACGCACC 
AATGACCCAG AGTACCAGGC TCAAGGCCTG GCCATGTACC TTCAGGAAAA CGGCATAGAC 
TGCCCCAAAT GCAAGTTCTC ATACGCACTG GCCCGAGGAG GCTGCATGCA CTTCCACTGC 
ACACAGTGTC GCCACCAGTT CTGCAGCGGC TGCTACAATG CCTTTTACGC CAAGAATAAA 
TGTCCAGACC CTAACTGCAA GGTGAAAAAG TCCCTGCATG GTCACCACCC TCGAGATTGC 
CTCTTCTACC TACGGGATTG GCCTGCTGCC CGGCTCCAGA AACTTTTGCA GGACAATAAT 
GTCATGTTTA ACACAGAGCC TCCTGCCGGC ACACGGGCAG TCCCTGGAGG TGGCTGCCGA 
GTGATGGAAC AGAAGGAGGT TCCCAGTGGG TTCAGGGATG AAGCTTGTGG CAAGGAAACT 
CCGCCTGGCT ATGCCGGCCT GTGTCAGGCA CACTACAAAG AGTATCTTGT GAGCCTCATC 
AATGCCCACT CGCTGGACCC AGCGACCCTG TATGAAGTGG AGGAACTGGA GACAGCCACT 
GCACGCTACC TACATTTAAC GCCCCAACCC ATGGATGGAG AGGATCTTCC TGCTTACCAG 
GCCCGGTTGT TACAGAAGCT GAGAGAAGAG GTGCCCTTGG GACAGAGTAT TGCTCGCAGA 
AGAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnf31 ( XM_006252000.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252062.1 Rattus norvegicus ring finger protein 31 (Rnf31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36694
Clone ID Related Accession (Same CDS sequence)	XM_006252000.3
Accession Version	XM_006252000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGAG ACGAGGAGCG AGCCTTCCTA GCGGCCCGCG AGGAGCTGGC GAGCGCCCTG 
AGGTGGGATT CTGCGCAGGT ATTTCCCCTG GAGCAGCTCA CGCCGCTTCT GGCCACCTCT 
CTGCCACTCT CCTCCCGCTA CCGGCAGCTG GATGCCGGAC GCCTGGTCCG CTGCAACGCT 
CATGGGGAGC CTCGAAACTA CCTCAACACT CTATCCACGG CCCTGAACAT CCTGGAAAAG 
TATGGTCGAA ACCTCCTCAG CCCACAGCGG CCCCGGTATT GGCGCTCAGT GAAGTTTAAT 
AACCCTGTCT TTCGCAGCAC GGTGGATGCT GTGCAGGGTG GCCGGGATGT GCTACGGTTG 
TATGGCTATA CCGAGGAGCG CCCAGATGGA TTGAGTTTCC CCGGAGGGCA GGAGGAACCA 
GATGAATACC AGGTTGCCAT AGTCACACTA GAAGTACTAC TGCTTCGCAC GGAGCTCAAT 
TTGCTGTTGC AGAATACTCA TCCAAGACAG AACGCACTGG ACCAGCTACT AAGAGACAGC 
GTTGAAGATG ACATGCTGCA GCTTTCAGAG TTTCACCCCC TTTTGAGAGA GATTGTTCCT 
GGCCCCCTCC CCTCTGCCCC AGGCCATACT CCTGGTCCCT GCTTTCTCTG TGGTTCTGCC 
CCAGGCACAC TGCACTGTCC AGCCTGTAAC CAAGCTTCAT GCCCAGCTTG TGACCTTTTG 
TTCCATGGGC ATCCGTCCCG TGCGCATCAC CTTCGCCACA CCCTACCCGG GGCCCACCAG 
ACCACCAACC TAAGCTCTAG TTTACCTGCC TCGTCCCAAC CACGGCCCCA CTCCTCCTCC 
CTGGCCATGG GAGATAGCTC TCTCTCTTCC CCTGACCCTG CAAATGCTTG TCTGCCCTGG 
CACTGTCCTA CCTGTGCCAC ATTAAACGAG CCTTGGGCAG TGTTCTGTGC AGCCTGTAGT 
CAGCCCAAAG GCTGTAAGGT GCGGGGAAGA GAGAGTTCCC AAGGAACTGG GGTCCTAGAG 
CCCGAGCCTG CACGGGATCA GTGGGCCTGC CAGAGCTGTA CCTTTGAGAA TGAGGCAGCA 
GCCGTGCTCT GTGCCATCTG TGAGCGACCT CGGCTGGCCC AGCCTCCCAG CTTGGTGGTG 
GATTCCCATG ACGCTGGTGT TTACCAACAG CCCCTTAAGG AGGATACTTT GCTCTCCGCT 
GCCCAGTCTC AGGTGTGGCA CTGTGACCAT TGTACCTTCT GCAATTCAGG CCCTGTCTGG 
GTGTGTGCCA TGTGTAACCG GACTCGCAGC CCCATCCCTG CACAGCCCTA TCCCAGCTCC 
TTGGAGAAGG GACTCTCACA GCCAGGGTCC TCACAACACC TCAGCTCCTC CCTGCCTGCT 
TCCTGTGGAG ATCCAGAGAA ACAGCGCCAA GATAAGATGC GGAAGGAAAG TCTCCAGCTA 
GTGAACATGA TCCAGGAAGG GGAAGCTGCA GGTGCCAGTC CAGAAGAGAT CTTCTCGGCT 
CTACAATACT CGGGCACTGA GGTGCCCCTG CAGTGGTTGC GTTCAGAGCT GTCCTACGTC 
TTGGAGATGG TGGCTGAGCT AGCTGGACAA CGGGATCCAG AGCTCGGTGC CTTTTCCTGT 
CAGGAAGCCC GGAAAGCCTG GCTTGATCGT CATGGCAACC TTGATGAAGC TGTAGAGGAG 
TGTGTGAGGG CCAGGAGGAG GAAGGTGCAG GAGCTCCAGT CCCTGGGCTT TGAGCCTAAA 
GAAGGGTCGC TGCAGGCATT GTTCCAGCAT GGGGGTGATG TGGCCCGGGC CCTAACTGAG 
TTACAGCGAC AGCGCCTGGA GCCTTTCCAC CAGCGCCTCT GGGGCAGAGA CCCCGAACCC 
ACTCCCTGCT GGGATGGCCT GGATAGACAG AGCCTGGTCA GACGCCTTCT GGCGGTATAC 
ACACTCCCCA GCTGGGGTCG AGCAGAGTTG GCGTTAGCGC TGCTGCAGGA GACACCCAGG 
AACTATGAGT TGTTGGACGT GGTGGAGGCC GTGAGGCACA GTCAGGACCG GGCCTTTCTG 
CGCCGACTGC TTGCCCAGGA ATGTGCTGTG TGCGGGTGGG CCCTTCCCCG AAACCGGATG 
CAGGCCCTGA TCTCCTGCGA GTGCACCATA TGCCCTGAGT GTTTCCGCCA GCATTTCACC 
ATTGCCCTGA AGGAGAAGCA CATCACAGAC ATGGTGTGCC CTGCCTGTGG CCGCCCTGAC 
CTCACCGATG ATGCGCAGTT ACTCAGTTAC TTCTCCACCC TTGACATCCA GCTCAGAGAG 
AGCCTAGAGC CGGATGCATA TGCTCTGTTT CATAAGAAGC TAACCGAGGC TGTGCTCATG 
CGAGACCCCA AGTTCTTGTG GTGTGCCCAG TGTTCCTTCG GCTTCATATA TGAACGAGAA 
CAGCTGGAGG CGACATGTCC CCAGTGTCAC CAGACCTTCT GTGTGCGCTG CAAGCGCCAG 
TGGGAGGAGC AGCACAGAGG GCGGAGCTGT GAGGATTTCC AGAACTGGAA ACGCACCAAT 
GACCCAGAGT ACCAGGCTCA AGGCCTGGCC ATGTACCTTC AGGAAAACGG CATAGACTGC 
CCCAAATGCA AGTTCTCATA CGCACTGGCC CGAGGAGGCT GCATGCACTT CCACTGCACA 
CAGTGTCGCC ACCAGTTCTG CAGCGGCTGC TACAATGCCT TTTACGCCAA GAATAAATGT 
CCAGACCCTA ACTGCAAGGT GAAAAAGTCC CTGCATGGTC ACCACCCTCG AGATTGCCTC 
TTCTACCTAC GGGATTGGCC TGCTGCCCGG CTCCAGAAAC TTTTGCAGGA CAATAATGTC 
ATGTTTAACA CAGAGCCTCC TGCCGGCACA CGGGCAGTCC CTGGAGGTGG CTGCCGAGTG 
ATGGAACAGA AGGAGGTTCC CAGTGGGTTC AGGGATGAAG CTTGTGGCAA GGAAACTCCG 
CCTGGCTATG CCGGCCTGTG TCAGGCACAC TACAAAGAGT ATCTTGTGAG CCTCATCAAT 
GCCCACTCGC TGGACCCAGC GACCCTGTAT GAAGTGGAGG AACTGGAGAC AGCCACTGCA 
CGCTACCTAC ATTTAACGCC CCAACCCATG GATGGAGAGG ATCTTCCTGC TTACCAGGCC 
CGGTTGTTAC AGAAGCTGAG AGAAGAGGTG CCCTTGGGAC AGAGTATTGC TCGCAGAAGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252062.1
CDS	805..3990
Translation	MPGDEERAFLAAREELASALRWDSAQVFPLEQLTPLLATSLPLSSRYRQLDAGRLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRSVKFNNPVFRSTVDAVQGGRDVLRLYGYTEERPDGLSFPGGQEEPDEYQVAIVTLEVLLLRTELNLLLQNTHPRQNALDQLLRDSVEDDMLQLSEFHPLLREIVPGPLPSAPGHTPGPCFLCGSAPGTLHCPACNQASCPACDLLFHGHPSRAHHLRHTLPGAHQTTNLSSSLPASSQPRPHSSSLAMGDSSLSSPDPANACLPWHCPTCATLNEPWAVFCAACSQPKGCKVRGRESSQGTGVLEPEPARDQWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSHDAGVYQQPLKEDTLLSAAQSQVWHCDHCTFCNSGPVWVCAMCNRTRSPIPAQPYPSSLEKGLSQPGSSQHLSSSLPASCGDPEKQRQDKMRKESLQLVNMIQEGEAAGASPEEIFSALQYSGTEVPLQWLRSELSYVLEMVAELAGQRDPELGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELQSLGFEPKEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWGRDPEPTPCWDGLDRQSLVRRLLAVYTLPSWGRAELALALLQETPRNYELLDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALISCECTICPECFRQHFTIALKEKHITDMVCPACGRPDLTDDAQLLSYFSTLDIQLRESLEPDAYALFHKKLTEAVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRTNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCKVKKSLHGHHPRDCLFYLRDWPAARLQKLLQDNNVMFNTEPPAGTRAVPGGGCRVMEQKEVPSGFRDEACGKETPPGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATARYLHLTPQPMDGEDLPAYQARLLQKLREEVPLGQSIARRRK

Target ORF information:

RefSeq Version	XM_006252000.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 31 (Rnf31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252000.3

ORF Insert Sequence:

ATGCCAGGAG ACGAGGAGCG AGCCTTCCTA GCGGCCCGCG AGGAGCTGGC GAGCGCCCTG 
AGGTGGGATT CTGCGCAGGT ATTTCCCCTG GAGCAGCTCA CGCCGCTTCT GGCCACCTCT 
CTGCCACTCT CCTCCCGCTA CCGGCAGCTG GATGCCGGAC GCCTGGTCCG CTGCAACGCT 
CATGGGGAGC CTCGAAACTA CCTCAACACT CTATCCACGG CCCTGAACAT CCTGGAAAAG 
TATGGTCGAA ACCTCCTCAG CCCACAGCGG CCCCGGTATT GGCGCTCAGT GAAGTTTAAT 
AACCCTGTCT TTCGCAGCAC GGTGGATGCT GTGCAGGGTG GCCGGGATGT GCTACGGTTG 
TATGGCTATA CCGAGGAGCG CCCAGATGGA TTGAGTTTCC CCGGAGGGCA GGAGGAACCA 
GATGAATACC AGGTTGCCAT AGTCACACTA GAAGTACTAC TGCTTCGCAC GGAGCTCAAT 
TTGCTGTTGC AGAATACTCA TCCAAGACAG AACGCACTGG ACCAGCTACT AAGAGACAGC 
GTTGAAGATG ACATGCTGCA GCTTTCAGAG TTTCACCCCC TTTTGAGAGA GATTGTTCCT 
GGCCCCCTCC CCTCTGCCCC AGGCCATACT CCTGGTCCCT GCTTTCTCTG TGGTTCTGCC 
CCAGGCACAC TGCACTGTCC AGCCTGTAAC CAAGCTTCAT GCCCAGCTTG TGACCTTTTG 
TTCCATGGGC ATCCGTCCCG TGCGCATCAC CTTCGCCACA CCCTACCCGG GGCCCACCAG 
ACCACCAACC TAAGCTCTAG TTTACCTGCC TCGTCCCAAC CACGGCCCCA CTCCTCCTCC 
CTGGCCATGG GAGATAGCTC TCTCTCTTCC CCTGACCCTG CAAATGCTTG TCTGCCCTGG 
CACTGTCCTA CCTGTGCCAC ATTAAACGAG CCTTGGGCAG TGTTCTGTGC AGCCTGTAGT 
CAGCCCAAAG GCTGTAAGGT GCGGGGAAGA GAGAGTTCCC AAGGAACTGG GGTCCTAGAG 
CCCGAGCCTG CACGGGATCA GTGGGCCTGC CAGAGCTGTA CCTTTGAGAA TGAGGCAGCA 
GCCGTGCTCT GTGCCATCTG TGAGCGACCT CGGCTGGCCC AGCCTCCCAG CTTGGTGGTG 
GATTCCCATG ACGCTGGTGT TTACCAACAG CCCCTTAAGG AGGATACTTT GCTCTCCGCT 
GCCCAGTCTC AGGTGTGGCA CTGTGACCAT TGTACCTTCT GCAATTCAGG CCCTGTCTGG 
GTGTGTGCCA TGTGTAACCG GACTCGCAGC CCCATCCCTG CACAGCCCTA TCCCAGCTCC 
TTGGAGAAGG GACTCTCACA GCCAGGGTCC TCACAACACC TCAGCTCCTC CCTGCCTGCT 
TCCTGTGGAG ATCCAGAGAA ACAGCGCCAA GATAAGATGC GGAAGGAAAG TCTCCAGCTA 
GTGAACATGA TCCAGGAAGG GGAAGCTGCA GGTGCCAGTC CAGAAGAGAT CTTCTCGGCT 
CTACAATACT CGGGCACTGA GGTGCCCCTG CAGTGGTTGC GTTCAGAGCT GTCCTACGTC 
TTGGAGATGG TGGCTGAGCT AGCTGGACAA CGGGATCCAG AGCTCGGTGC CTTTTCCTGT 
CAGGAAGCCC GGAAAGCCTG GCTTGATCGT CATGGCAACC TTGATGAAGC TGTAGAGGAG 
TGTGTGAGGG CCAGGAGGAG GAAGGTGCAG GAGCTCCAGT CCCTGGGCTT TGAGCCTAAA 
GAAGGGTCGC TGCAGGCATT GTTCCAGCAT GGGGGTGATG TGGCCCGGGC CCTAACTGAG 
TTACAGCGAC AGCGCCTGGA GCCTTTCCAC CAGCGCCTCT GGGGCAGAGA CCCCGAACCC 
ACTCCCTGCT GGGATGGCCT GGATAGACAG AGCCTGGTCA GACGCCTTCT GGCGGTATAC 
ACACTCCCCA GCTGGGGTCG AGCAGAGTTG GCGTTAGCGC TGCTGCAGGA GACACCCAGG 
AACTATGAGT TGTTGGACGT GGTGGAGGCC GTGAGGCACA GTCAGGACCG GGCCTTTCTG 
CGCCGACTGC TTGCCCAGGA ATGTGCTGTG TGCGGGTGGG CCCTTCCCCG AAACCGGATG 
CAGGCCCTGA TCTCCTGCGA GTGCACCATA TGCCCTGAGT GTTTCCGCCA GCATTTCACC 
ATTGCCCTGA AGGAGAAGCA CATCACAGAC ATGGTGTGCC CTGCCTGTGG CCGCCCTGAC 
CTCACCGATG ATGCGCAGTT ACTCAGTTAC TTCTCCACCC TTGACATCCA GCTCAGAGAG 
AGCCTAGAGC CGGATGCATA TGCTCTGTTT CATAAGAAGC TAACCGAGGC TGTGCTCATG 
CGAGACCCCA AGTTCTTGTG GTGTGCCCAG TGTTCCTTCG GCTTCATATA TGAACGAGAA 
CAGCTGGAGG CGACATGTCC CCAGTGTCAC CAGACCTTCT GTGTGCGCTG CAAGCGCCAG 
TGGGAGGAGC AGCACAGAGG GCGGAGCTGT GAGGATTTCC AGAACTGGAA ACGCACCAAT 
GACCCAGAGT ACCAGGCTCA AGGCCTGGCC ATGTACCTTC AGGAAAACGG CATAGACTGC 
CCCAAATGCA AGTTCTCATA CGCACTGGCC CGAGGAGGCT GCATGCACTT CCACTGCACA 
CAGTGTCGCC ACCAGTTCTG CAGCGGCTGC TACAATGCCT TTTACGCCAA GAATAAATGT 
CCAGACCCTA ACTGCAAGGT GAAAAAGTCC CTGCATGGTC ACCACCCTCG AGATTGCCTC 
TTCTACCTAC GGGATTGGCC TGCTGCCCGG CTCCAGAAAC TTTTGCAGGA CAATAATGTC 
ATGTTTAACA CAGAGCCTCC TGCCGGCACA CGGGCAGTCC CTGGAGGTGG CTGCCGAGTG 
ATGGAACAGA AGGAGGTTCC CAGTGGGTTC AGGGATGAAG CTTGTGGCAA GGAAACTCCG 
CCTGGCTATG CCGGCCTGTG TCAGGCACAC TACAAAGAGT ATCTTGTGAG CCTCATCAAT 
GCCCACTCGC TGGACCCAGC GACCCTGTAT GAAGTGGAGG AACTGGAGAC AGCCACTGCA 
CGCTACCTAC ATTTAACGCC CCAACCCATG GATGGAGAGG ATCTTCCTGC TTACCAGGCC 
CGGTTGTTAC AGAAGCTGAG AGAAGAGGTG CCCTTGGGAC AGAGTATTGC TCGCAGAAGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.
Cell166(5)1215-1230.e20(2016 Aug)
Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Molecular basis and regulation of OTULIN-LUBAC interaction.
Molecular cell54(3)335-48(2014 May)
Elliott PR,Nielsen SV,Marco-Casanova P,Fiil BK,Keusekotten K,Mailand N,Freund SM,Gyrd-Hansen M,Komander D

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

The E3 ligase parkin maintains mitochondrial integrity by increasing linear ubiquitination of NEMO.
Molecular cell49(5)908-21(2013 Mar)
M?ller-Rischart AK,Pilsl A,Beaudette P,Patra M,Hadian K,Funke M,Peis R,Deinlein A,Schweimer C,Kuhn PH,Lichtenthaler SF,Motori E,Hrelia S,Wurst W,Tr?mbach D,Langer T,Krappmann D,Dittmar G,Tatzelt J,Winklhofer KF

Tumor necrosis factor.
Cancer letters328(2)222-5(2013 Jan)
Chu WM

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Rnf31 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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