Prmt5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prmt5
Entrez Gene ID	364382
Full Name	protein arginine methyltransferase 5
Synonyms	Hrmt1l5,Skb1
General protein information	Preferred Names protein arginine methyltransferase 5 Names protein arginine N-methyltransferase 5 HMT1 hnRNP methyltransferase-like 5 SKB1 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108867.2	NP_001102337.2	protein arginine N-methyltransferase 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRMT5	XP_509844.3
Canis lupus familiaris (dog)	PRMT5	XP_537366.2
Mus musculus (house mouse)	Prmt5	NP_038796.2
Danio rerio (zebrafish)	prmt5	NP_001007184.2
Xenopus tropicalis (tropical clawed frog)	prmt5	XP_002941557.1
Homo sapiens (human)	PRMT5	NP_006100.2
Drosophila melanogaster (fruit fly)	csul	NP_725552.1
Oryza sativa (rice)	Os02g0139200	NP_001045843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009048	XP_319800.4
	PRMT5	XP_001103964.1
Bos taurus (cattle)	PRMT5	NP_001098844.1
Rattus norvegicus (Norway rat)	Prmt5	NP_001102337.2
Arabidopsis thaliana (thale cress)	SKB1	NP_194841.2

Gene Ontology
Bibliography

Related articles in PubMed

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.
Zhao DY, Gish G, Braunschweig U, Li Y, Ni Z, Schmitges FW, Zhong G, Liu K, Li W, Moffat J, Vedadi M, Min J, Pawson TJ, Blencowe BJ, Greenblatt JF
Nature529(7584)48-53(2016 Jan)

The protein arginine methyltransferase PRMT5 promotes D2-like dopamine receptor signaling.
Likhite N, Jackson CA, Liang MS, Krzyzanowski MC, Lei P, Wood JF, Birkaya B, Michaels KL, Andreadis ST, Clark SD, Yu MC, Ferkey DM
Science signaling8(402)ra115(2015 Nov)

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Takai H, Masuda K, Sato T, Sakaguchi Y, Suzuki T, Suzuki T, Koyama-Nasu R, Nasu-Nishimura Y, Katou Y, Ogawa H, Morishita Y, Kozuka-Hata H, Oyama M, Todo T, Ino Y, Mukasa A, Saito N, Toyoshima C, Shirahige K, Akiyama T
Cell reports9(1)48-60(2014 Oct)

Readers of histone methylarginine marks.
Gayatri S, Bedford MT
Biochimica et biophysica acta1839(8)702-10(2014 Aug)

Role of type II protein arginine methyltransferase 5 in the regulation of Circadian Per1 gene.
Na J, Lee K, Kim HG, Shin JY, Na W, Jeong H, Lee JW, Cho S, Kim WS, Ju BG
PloS one7(10)e48152(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell
Title: Type II arginine methyltransferase PRMT5 regulates gene expression of inhibitors of differentiation/DNA binding Id2 and Id4 during glial cell differentiation.

analysis of structural insights into protein arginine symmetric dimethylation by PRMT5
Title: Structural insights into protein arginine symmetric dimethylation by PRMT5.

Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF.
Title: Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF.

These data provide the first direct experimental evidence that PRMT5, a type II arginine methylase, is involved in the control of transcription and proliferation.
Title: Negative regulation of transcription by the type II arginine methyltransferase PRMT5.

The following Prmt5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prmt5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08527	NM_001108867.2 Latest version!	Rattus norvegicus protein arginine methyltransferase 5 (Prmt5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$377.30 $539.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prmt5 ( NM_001108867.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102337.2 Rattus norvegicus protein arginine methyltransferase 5 (Prmt5), mRNA.

Price & Availability↑

CloneID	ORa08527
Clone ID Related Accession (Same CDS sequence)	NM_001108867.2
Accession Version	NM_001108867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	protein arginine N-methyltransferase 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB814013.1, CO573950.1, DY553859.1, CB793692.1, CK845516.1, BQ210423.1, BE116721.1 and AI713913.1. On Apr 21, 2008 this sequence version replaced NM_001108867.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGA TGGCAGTCGG AGGTGCTGGT GGCAGCCGCG TGTCCAGCGG GAGGGACCTG 
AATTGCGTCC CTGAAATAGC TGACACACTG GGTGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG 
AATCGGCCTG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CATCCAGACT CAAAAGTGGA GAAGATCCGA 
AGGAACTCTG AGGCGGCTAT GTTGCAGGAG CTGAATTTTG GGGCATATCT AGGTCTTCCA 
GCTTTCCTAT TGCCCCTTAA TCAGGAAGAT AACACAAATC TGGCCAGAGT TCTGACCAAC 
CACATCCACA CTGGCCACCA TTCTTCCATG TTCTGGATGA GGGTCCCCTT GGTGGCCCCA 
GAGGACCTGA GAGATGATGT AATTGAGAAT GCACCAACTG CACACACAGA GGAGTACAGT 
GGAGAGGAGA AGACGTGGTT GTGGTGGCAT AACTTCCGGA CTCTGTGTGA CTATAGTAAG 
AGGATTGCAG TAGCTCTTGA AATCGGAGCT GACCTCCCGT CTAGTCACGT CATTGATCGT 
TGGCTTGGAG AGCCCATCAA AGCCGCCATT CTCCCCACCA GCATTTTCCT AACCAACAAG 
AAGGGGTTTC CTGTTCTTTC TAAGATGCAG CAGAGGCTGA TCTTCCGGCT CCTCAAGTTG 
GAAGTGCAAT TTATCATCAC GGGAACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC 
CTCCAGTACT TGGAATACTT AAGCCAAAAT CGTCCCCCAC CCAATGCTTA CGAACTCTTT 
GCCAAAGGCT ATGAAGACTA TCTGCAGTCC CCACTTCAGC CTCTGATGGA CAATCTGGAA 
TCTCAGACGT ACGAAGTGTT CGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC 
ATCTATAAAT GTTTGCTAGA CCGAGTACCA GAAGAAGAAA AGGAGACCAA TGTCCAGGTA 
CTTATGGTGC TGGGTGCAGG CCGAGGTCCT CTAGTGAATG CGTCTCTCCG GGCAGCCAAG 
CAGGCTGAGC GGCGGATTAG GCTGTATGCC GTGGAGAAGA ACCCCAATGC TGTGGTGACG 
CTAGAGAACT GGCAGTTTGA AGAATGGGGG AGTCAGGTGA CAGTTGTCTC GTCAGACATG 
CGGGAATGGG TGGCTCCGGA GAAGGCAGAC ATCATTGTCA GTGAACTTCT GGGCTCCTTT 
GCCGACAATG AGCTGTCACC TGAGTGTCTG GATGGAGCAC AGCACTTCCT GAAAGATGAT 
GGTGTAAGCA TCCCCGGAGA ATACACCTCC TTCCTGGCTC CCATTTCTTC CTCGAAGTTG 
TACAACGAGG TCCGCGCCTG TCGGGAGAAG GACCGTGACC CTGAGGCACA GTTTGAGATG 
CCTTATGTGG TTCGGCTGCA CAACTTCCAC CAGCTTTCTG CTCCTCAGCC CTGTTTTACC 
TTCAGCCATC CCAACCGAGA TCCTATGATT GACAACAACC GCTACTGTAC CTTGGAGTTT 
CCTGTGGAGG TGAACACGGT GCTTCATGGC TTTGCAGGCT ACTTTGAAAC TGTGCTTTAC 
CGGGACATCA CTCTGAGTAT CCGCCCAGAG ACCCACTCTC CTGGCATGTT CTCATGGTTC 
CCCATCTTCT TCCCCATCAA GCAGCCCATC ACGGTGCATG AAGGCCAGAA CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCTAAGAAG GTGTGGTACG AGTGGGCGGT GACAGCGCCA 
GTCTGTTCTT CTATTCACAA CCCTACTGGT CGCTCCTATA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102337.2
CDS	15..1928
Translation	MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIHPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDVIENAPTAHTEEYSGEEKTWLWWHNFRTLCDYSKRIAVALEIGADLPSSHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMQQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPEEEKETNVQVLMVLGAGRGPLVNASLRAAKQAERRIRLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYRDITLSIRPETHSPGMFSWFPIFFPIKQPITVHEGQNICVRFWRCSNSKKVWYEWAVTAPVCSSIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	NM_001108867.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein arginine methyltransferase 5 (Prmt5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108867.2

ORF Insert Sequence:

ATGGCGGCGA TGGCAGTCGG AGGTGCTGGT GGCAGCCGCG TGTCCAGCGG GAGGGACCTG 
AATTGCGTCC CTGAAATAGC TGACACACTG GGTGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCATTCAGGA ACCTGCTAAG 
AATCGGCCTG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CATCCAGACT CAAAAGTGGA GAAGATCCGA 
AGGAACTCTG AGGCGGCTAT GTTGCAGGAG CTGAATTTTG GGGCATATCT AGGTCTTCCA 
GCTTTCCTAT TGCCCCTTAA TCAGGAAGAT AACACAAATC TGGCCAGAGT TCTGACCAAC 
CACATCCACA CTGGCCACCA TTCTTCCATG TTCTGGATGA GGGTCCCCTT GGTGGCCCCA 
GAGGACCTGA GAGATGATGT AATTGAGAAT GCACCAACTG CACACACAGA GGAGTACAGT 
GGAGAGGAGA AGACGTGGTT GTGGTGGCAT AACTTCCGGA CTCTGTGTGA CTATAGTAAG 
AGGATTGCAG TAGCTCTTGA AATCGGAGCT GACCTCCCGT CTAGTCACGT CATTGATCGT 
TGGCTTGGAG AGCCCATCAA AGCCGCCATT CTCCCCACCA GCATTTTCCT AACCAACAAG 
AAGGGGTTTC CTGTTCTTTC TAAGATGCAG CAGAGGCTGA TCTTCCGGCT CCTCAAGTTG 
GAAGTGCAAT TTATCATCAC GGGAACCAAC CACCACTCAG AGAAGGAGTT CTGCTCCTAC 
CTCCAGTACT TGGAATACTT AAGCCAAAAT CGTCCCCCAC CCAATGCTTA CGAACTCTTT 
GCCAAAGGCT ATGAAGACTA TCTGCAGTCC CCACTTCAGC CTCTGATGGA CAATCTGGAA 
TCTCAGACGT ACGAAGTGTT CGAAAAGGAC CCCATCAAAT ACTCTCAGTA CCAGCAGGCC 
ATCTATAAAT GTTTGCTAGA CCGAGTACCA GAAGAAGAAA AGGAGACCAA TGTCCAGGTA 
CTTATGGTGC TGGGTGCAGG CCGAGGTCCT CTAGTGAATG CGTCTCTCCG GGCAGCCAAG 
CAGGCTGAGC GGCGGATTAG GCTGTATGCC GTGGAGAAGA ACCCCAATGC TGTGGTGACG 
CTAGAGAACT GGCAGTTTGA AGAATGGGGG AGTCAGGTGA CAGTTGTCTC GTCAGACATG 
CGGGAATGGG TGGCTCCGGA GAAGGCAGAC ATCATTGTCA GTGAACTTCT GGGCTCCTTT 
GCCGACAATG AGCTGTCACC TGAGTGTCTG GATGGAGCAC AGCACTTCCT GAAAGATGAT 
GGTGTAAGCA TCCCCGGAGA ATACACCTCC TTCCTGGCTC CCATTTCTTC CTCGAAGTTG 
TACAACGAGG TCCGCGCCTG TCGGGAGAAG GACCGTGACC CTGAGGCACA GTTTGAGATG 
CCTTATGTGG TTCGGCTGCA CAACTTCCAC CAGCTTTCTG CTCCTCAGCC CTGTTTTACC 
TTCAGCCATC CCAACCGAGA TCCTATGATT GACAACAACC GCTACTGTAC CTTGGAGTTT 
CCTGTGGAGG TGAACACGGT GCTTCATGGC TTTGCAGGCT ACTTTGAAAC TGTGCTTTAC 
CGGGACATCA CTCTGAGTAT CCGCCCAGAG ACCCACTCTC CTGGCATGTT CTCATGGTTC 
CCCATCTTCT TCCCCATCAA GCAGCCCATC ACGGTGCATG AAGGCCAGAA CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCTAAGAAG GTGTGGTACG AGTGGGCGGT GACAGCGCCA 
GTCTGTTCTT CTATTCACAA CCCTACTGGT CGCTCCTATA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SMN and symmetric arginine dimethylation of RNA polymerase II C-terminal domain control termination.
Nature529(7584)48-53(2016 Jan)
Zhao DY,Gish G,Braunschweig U,Li Y,Ni Z,Schmitges FW,Zhong G,Liu K,Li W,Moffat J,Vedadi M,Min J,Pawson TJ,Blencowe BJ,Greenblatt JF

The protein arginine methyltransferase PRMT5 promotes D2-like dopamine receptor signaling.
Science signaling8(402)ra115(2015 Nov)
Likhite N,Jackson CA,Liang MS,Krzyzanowski MC,Lei P,Wood JF,Birkaya B,Michaels KL,Andreadis ST,Clark SD,Yu MC,Ferkey DM

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Cell reports9(1)48-60(2014 Oct)
Takai H,Masuda K,Sato T,Sakaguchi Y,Suzuki T,Suzuki T,Koyama-Nasu R,Nasu-Nishimura Y,Katou Y,Ogawa H,Morishita Y,Kozuka-Hata H,Oyama M,Todo T,Ino Y,Mukasa A,Saito N,Toyoshima C,Shirahige K,Akiyama T

Readers of histone methylarginine marks.
Biochimica et biophysica acta1839(8)702-10(2014 Aug)
Gayatri S,Bedford MT

Role of type II protein arginine methyltransferase 5 in the regulation of Circadian Per1 gene.
PloS one7(10)e48152(2012)
Na J,Lee K,Kim HG,Shin JY,Na W,Jeong H,Lee JW,Cho S,Kim WS,Ju BG

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Prmt5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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