Myl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myl2
Entrez Gene ID	363925
Full Name	myosin light chain 2
Synonyms	MLC-2s/v,Mlc-2,Mlc2
General protein information	Preferred Names myosin light chain 2 Names myosin regulatory light chain 2, ventricular/cardiac muscle isoform cardiac myosin light chain-2 myosin light chain 2, slow skeletal/ventricular muscle isoform myosin, light chain 2, regulatory, cardiac, slow myosin, light polypeptide 2, regulatory, cardiac, slow
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001035252.2	NP_001030329.2	myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myl2	NP_001030329.2
Gallus gallus (chicken)	MYL2	NP_001258858.1
Canis lupus familiaris (dog)	MYL2	NP_001003069.1
Danio rerio (zebrafish)	myl2b	NP_001035134.1
Danio rerio (zebrafish)	LOC100537244	XP_003198611.2
Xenopus tropicalis (tropical clawed frog)	myl2	NP_001107717.1
Pan troglodytes (chimpanzee)	MYL2	XP_001144828.2
Mus musculus (house mouse)	Myl2	NP_034991.3
Bos taurus (cattle)	MYL2	NP_001030197.1
Homo sapiens (human)	MYL2	NP_000423.2
	MYL2	XP_001100796.1

Gene Ontology
Bibliography

Related articles in PubMed

Cyclical compressive stress induces differentiation of rat primary mandibular condylar chondrocytes through phosphorylated myosin light chain II.
Liu L, Chen L, Mai Z, Peng Z, Yu K, Liu G, Ai H
Molecular medicine reports14(5)4293-4300(2016 Nov)

A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis.
Wijnker PJ, Li Y, Zhang P, Foster DB, dos Remedios C, Van Eyk JE, Stienen GJ, Murphy AM, van der Velden J
Journal of molecular and cellular cardiology8293-103(2015 May)

Nuclear cardiac myosin light chain 2 modulates NADPH oxidase 2 expression in myocardium: a novel function beyond muscle contraction.
Zhang YS, Liu B, Luo XJ, Li TB, Zhang JJ, Peng JJ, Zhang XJ, Ma QL, Hu CP, Li YJ, Peng J, Li Q
Basic research in cardiology110(4)38(2015)

Proteome expression and carbonylation changes during Trypanosoma cruzi infection and Chagas disease in rats.
Wen JJ, Garg NJ
Molecular & cellular proteomics : MCP11(4)M111.010918(2012 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing MLCII by RNA interference reduced ALP activity (P<0.01), and eliminated RUNX2 mRNA expression (P<0.01). Addition of the MLC kinase inhibitor, ML7, reduced the CUCSassociated upregulation of RUNX2 expression (P<0.01) and ALP activity (P<0.01).
Title: Cyclical compressive stress induces differentiation of rat primary mandibular condylar chondrocytes through phosphorylated myosin light chain II.

nuclear MYL2 plays an important role in IR injury by transcriptionally upregulating NOX2 expression to enhance oxidative stress in a phosphorylation-dependent manner.
Title: Nuclear cardiac myosin light chain 2 modulates NADPH oxidase 2 expression in myocardium: a novel function beyond muscle contraction.

We have identified three protein biomarkers (GSN, MYL2, and VIM) that have potential utility in diagnosing the severity of Chagas disease
Title: Proteome expression and carbonylation changes during Trypanosoma cruzi infection and Chagas disease in rats.

Prostanoid F receptor (FPR) signalling mechanism(s) regulating myosin light chain-2 phosphorylation likely extend beyond those classically established for G(q/11)-coupled receptors.
Title: Prostanoid F receptors elicit an inotropic effect in rat left ventricle by enhancing myosin light chain phosphorylation.

The following Myl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42855	NM_001035252.2 Latest version!	Rattus norvegicus myosin light chain 2 (Myl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myl2 ( NM_001035252.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001030329.2 Rattus norvegicus myosin light chain 2 (Myl2), mRNA.

Price & Availability↑

CloneID	ORa42855
Clone ID Related Accession (Same CDS sequence)	NM_001035252.2
Accession Version	NM_001035252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126064.1. On May 10, 2012 this sequence version replaced NM_001035252.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGTCACCAA AGAAAGCCAA GAAGAGGTTA GAGGGCGGAA GCTCCAACGT GTTCTCCATG 
TTTGAGCAGA CCCAGATCCA GGAGTTCAAG GAGGCCTTCA CAATCATGGA CCAGAACAGA 
GACGGCTTCA TTGACAAGAA TGACCTAAGG GACACGTTTG CTGCCCTCGG ACGAGTGAAC 
GTGAAAAACG AAGAGATCGA TGAGATGATC AAAGAGGCTC CAGGTCCAAT TAACTTCACT 
GTGTTCCTCA CCATGTTTGG GGAGAAACTT AAAGGAGCTG ACCCGGAGGA GACCATTCTC 
AACGCCTTCA AGGTGTTTGA CCCCGAAGGC AAAGGGTCGC TGAAGGCCGA CTATGTCCGG 
GAGATGCTGA CCACGCAAGC AGAGAGGTTC TCCAAAGAAG AGATCGACCA GATGTTCGCA 
GCTTTTCCCC CTGACGTCAC CGGCAACCTT GATTATAAGA ATTTGGTTCA CATCATCACC 
CACGGAGAAG AGAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030329.2
CDS	30..530
Translation	MSPKKAKKRLEGGSSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGSLKADYVREMLTTQAERFSKEEIDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Target ORF information:

RefSeq Version	NM_001035252.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin light chain 2 (Myl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001035252.2

ORF Insert Sequence:

ATGTCACCAA AGAAAGCCAA GAAGAGGTTA GAGGGCGGAA GCTCCAACGT GTTCTCCATG 
TTTGAGCAGA CCCAGATCCA GGAGTTCAAG GAGGCCTTCA CAATCATGGA CCAGAACAGA 
GACGGCTTCA TTGACAAGAA TGACCTAAGG GACACGTTTG CTGCCCTCGG ACGAGTGAAC 
GTGAAAAACG AAGAGATCGA TGAGATGATC AAAGAGGCTC CAGGTCCAAT TAACTTCACT 
GTGTTCCTCA CCATGTTTGG GGAGAAACTT AAAGGAGCTG ACCCGGAGGA GACCATTCTC 
AACGCCTTCA AGGTGTTTGA CCCCGAAGGC AAAGGGTCGC TGAAGGCCGA CTATGTCCGG 
GAGATGCTGA CCACGCAAGC AGAGAGGTTC TCCAAAGAAG AGATCGACCA GATGTTCGCA 
GCTTTTCCCC CTGACGTCAC CGGCAACCTT GATTATAAGA ATTTGGTTCA CATCATCACC 
CACGGAGAAG AGAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cyclical compressive stress induces differentiation of rat primary mandibular condylar chondrocytes through phosphorylated myosin light chain II.
Molecular medicine reports14(5)4293-4300(2016 Nov)
Liu L,Chen L,Mai Z,Peng Z,Yu K,Liu G,Ai H

A novel phosphorylation site, Serine 199, in the C-terminus of cardiac troponin I regulates calcium sensitivity and susceptibility to calpain-induced proteolysis.
Journal of molecular and cellular cardiology8293-103(2015 May)
Wijnker PJ,Li Y,Zhang P,Foster DB,dos Remedios C,Van Eyk JE,Stienen GJ,Murphy AM,van der Velden J

Nuclear cardiac myosin light chain 2 modulates NADPH oxidase 2 expression in myocardium: a novel function beyond muscle contraction.
Basic research in cardiology110(4)38(2015)
Zhang YS,Liu B,Luo XJ,Li TB,Zhang JJ,Peng JJ,Zhang XJ,Ma QL,Hu CP,Li YJ,Peng J,Li Q

Proteome expression and carbonylation changes during Trypanosoma cruzi infection and Chagas disease in rats.
Molecular & cellular proteomics : MCP11(4)M111.010918(2012 Apr)
Wen JJ,Garg NJ