Ube2l3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2l3
Entrez Gene ID	363836
Full Name	ubiquitin-conjugating enzyme E2L 3
Synonyms	UbcH7,UbcR7
General protein information	Preferred Names ubiquitin-conjugating enzyme E2L 3 Names ubiquitin-conjugating enzyme E2 L3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329136.1	NP_001316065.1	ubiquitin-conjugating enzyme E2 L3
NM_001108847.1	NP_001102317.1	ubiquitin-conjugating enzyme E2 L3 isoform 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The three 'P's of mitophagy: PARKIN, PINK1, and post-translational modifications.
Durcan TM, Fon EA
Genes & development29(10)989-99(2015 May)

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Altered social behavior and neuronal development in mice lacking the Uba6-Use1 ubiquitin transfer system.
Lee PC, Dodart JC, Aron L, Finley LW, Bronson RT, Haigis MC, Yankner BA, Harper JW
Molecular cell50(2)172-84(2013 Apr)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.
Wenzel DM, Lissounov A, Brzovic PS, Klevit RE
Nature474(7349)105-8(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2l3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2l3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11860	NM_001108847.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa34493	NM_001329136.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2l3 ( NM_001108847.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102317.1 Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa11860
Clone ID Related Accession (Same CDS sequence)	NM_001108847.1
Accession Version	NM_001108847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 L3 isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473999.2. On or before Oct 4, 2007 this sequence version replaced XM_344046.3, XM_001067198.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC167087.1, EV772206.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAACTATG CTTTAGTTCT AGGTATGCAT GGGGCAGGTT TCTGGAGATT TCCATATGCC 
CAGGATGGTC AGAGCCCTGG GCCTGTGTTT CTATGTCTGT CTCTTCATTG GCAGAAAAGT 
GACAATGGAT TTTATTTCAC TCACATTCAG ATTTGTAATA AAATGCCAAA CTCTGTCAGA 
ATTGTCCAAA CCAACCACCA TTTTGTTGAT GTTGCTTTTC TGAATCTAAA GATAGTCCAG 
CTATCTCATT GCTTAGCTCT ACCTGCCTGT TACCCTCACT GTTCTCTGCT CATATGGGAG 
TACTCTTATG CCAGCAGCCC CTCTCAGGGA CTCTCAGCCC CACTGTTGAC AACCATGGCC 
TGCCCTGGAA GCAGAGGCCT GGCTTGGAGT CAGTGGTGTC CCTCTGGGCT TGCATGCCTT 
GTCCTTTCCA ACCCTGCCCT GGAGCAGGCT TTGACCAAGC TGGCAGGGAG ACCTGTGGAA 
CAGCCAGCCA CCCACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102317.1
CDS	1991..2491
Translation	MNYALVLGMHGAGFWRFPYAQDGQSPGPVFLCLSLHWQKSDNGFYFTHIQICNKMPNSVRIVQTNHHFVDVAFLNLKIVQLSHCLALPACYPHCSLLIWEYSYASSPSQGLSAPLLTTMACPGSRGLAWSQWCPSGLACLVLSNPALEQALTKLAGRPVEQPATHP

Target ORF information:

RefSeq Version	NM_001108847.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108847.1

ORF Insert Sequence:

ATGAACTATG CTTTAGTTCT AGGTATGCAT GGGGCAGGTT TCTGGAGATT TCCATATGCC 
CAGGATGGTC AGAGCCCTGG GCCTGTGTTT CTATGTCTGT CTCTTCATTG GCAGAAAAGT 
GACAATGGAT TTTATTTCAC TCACATTCAG ATTTGTAATA AAATGCCAAA CTCTGTCAGA 
ATTGTCCAAA CCAACCACCA TTTTGTTGAT GTTGCTTTTC TGAATCTAAA GATAGTCCAG 
CTATCTCATT GCTTAGCTCT ACCTGCCTGT TACCCTCACT GTTCTCTGCT CATATGGGAG 
TACTCTTATG CCAGCAGCCC CTCTCAGGGA CTCTCAGCCC CACTGTTGAC AACCATGGCC 
TGCCCTGGAA GCAGAGGCCT GGCTTGGAGT CAGTGGTGTC CCTCTGGGCT TGCATGCCTT 
GTCCTTTCCA ACCCTGCCCT GGAGCAGGCT TTGACCAAGC TGGCAGGGAG ACCTGTGGAA 
CAGCCAGCCA CCCACCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2l3 ( NM_001329136.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001316065.1 Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), mRNA.

Price & Availability↑

CloneID	ORa34493
Clone ID Related Accession (Same CDS sequence)	NM_001329136.1
Accession Version	NM_001329136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-17
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 L3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC128500.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ095739.1, FQ074932.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906352, SAMN02642739 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCA GCAGGAGGCT GATGAAGGAG CTTGAAGAGA TCCGCAAATG TGGAATGAAA 
AACTTCCGTA ACATCCAGGT TGATGAAGCT AATTTATTGA CTTGGCAAGG GCTTATTGTT 
CCTGACAACC CTCCATATGA TAAGGGAGCC TTCAGAATTG AGATCAACTT CCCAGCAGAG 
TATCCCTTCA AGCCACCCAA GATCACATTT AAAACAAAGA TCTACCACCC TAACATCGAC 
GAGAAGGGGC AGGTCTGTCT GCCCGTAATT AGTGCGGAAA ACTGGAAGCC GGCCACCAAG 
ACTGACCAAG TAATCCAGTC CCTCATAGCA CTGGTGAACG ACCCTCAGCC TGAGCACCCA 
CTCCGGGCTG ACCTAGCTGA AGAATACTCT AAGGACCGTA AAAAATTCTG TAAGAATGCT 
GAAGAGTTTA CAAAGAAATA TGGGGAAAAG CGACCTGTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316065.1
CDS	264..728
Translation	MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Target ORF information:

RefSeq Version	NM_001329136.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2L 3 (Ube2l3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329136.1

ORF Insert Sequence:

ATGGCGGCCA GCAGGAGGCT GATGAAGGAG CTTGAAGAGA TCCGCAAATG TGGAATGAAA 
AACTTCCGTA ACATCCAGGT TGATGAAGCT AATTTATTGA CTTGGCAAGG GCTTATTGTT 
CCTGACAACC CTCCATATGA TAAGGGAGCC TTCAGAATTG AGATCAACTT CCCAGCAGAG 
TATCCCTTCA AGCCACCCAA GATCACATTT AAAACAAAGA TCTACCACCC TAACATCGAC 
GAGAAGGGGC AGGTCTGTCT GCCCGTAATT AGTGCGGAAA ACTGGAAGCC GGCCACCAAG 
ACTGACCAAG TAATCCAGTC CCTCATAGCA CTGGTGAACG ACCCTCAGCC TGAGCACCCA 
CTCCGGGCTG ACCTAGCTGA AGAATACTCT AAGGACCGTA AAAAATTCTG TAAGAATGCT 
GAAGAGTTTA CAAAGAAATA TGGGGAAAAG CGACCTGTGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The three 'P's of mitophagy: PARKIN, PINK1, and post-translational modifications.
Genes & development29(10)989-99(2015 May)
Durcan TM,Fon EA

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Altered social behavior and neuronal development in mice lacking the Uba6-Use1 ubiquitin transfer system.
Molecular cell50(2)172-84(2013 Apr)
Lee PC,Dodart JC,Aron L,Finley LW,Bronson RT,Haigis MC,Yankner BA,Harper JW

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.
Nature474(7349)105-8(2011 Jun)
Wenzel DM,Lissounov A,Brzovic PS,Klevit RE