Selenoi cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Selenoi
Entrez Gene ID	362713
Full Name	selenoprotein I
Synonyms	Ept1,RGD1560938,Seli
General protein information	Preferred Names selenoprotein I Names ethanolaminephosphotransferase 1 ethanolaminephosphotransferase 1 (CDP-ethanolamine-specific)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134754.1	NP_001128226.2	ethanolaminephosphotransferase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPT1	NP_001140169.1
	EPT1	NP_001129035.1
Gallus gallus (chicken)	EPT1	NP_001026699.2
Caenorhabditis elegans (roundworm)	CELE_F54D7.2	NP_491454.2
Mus musculus (house mouse)	Ept1	NP_081928.2
Rattus norvegicus (Norway rat)	Seli	NP_001128226.2
Drosophila melanogaster (fruit fly)	CG33116	NP_788074.1
Xenopus tropicalis (tropical clawed frog)	ept1	NP_001004832.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008881	XP_319628.3
Homo sapiens (human)	EPT1	NP_277040.1
Canis lupus familiaris (dog)	EPT1	NP_001157958.1
Bos taurus (cattle)	EPT1	NP_001068725.2

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Identification and characterization of human ethanolaminephosphotransferase1.
Horibata Y, Hirabayashi Y
Journal of lipid research48(3)503-8(2007 Mar)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Selenoi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Selenoi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03565	NM_001134754.1 Latest version!	Rattus norvegicus selenoprotein I (Selenoi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Selenoi ( NM_001134754.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001128226.2 Rattus norvegicus selenoprotein I (Selenoi), mRNA.

Price & Availability↑

CloneID	ORa03565
Clone ID Related Accession (Same CDS sequence)	NM_001134754.1
Accession Version	NM_001134754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC168235.1. On or before Sep 5, 2008 this sequence version replaced XM_343031.3, XM_001069697.1. ##Evidence-Data-START## Transcript exon combination :: BC168235.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCT ACGAATACGT GAGCCCAGAG CAGCTGGCGG GCTTTGACAA GTATAAGTAC 
AGCGCTTTGG ATACCAACCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTATA 
GTGAAGGTAT TTCCCACTTG GCTGGCTCCC AATCTTATAA CCTTTTCTGG CTTTATGCTG 
CTTGTGTTCA ATTTCCTACT CCTGACATAC TTCGACCCTG ACTTCTATGC TTCAGCGCCA 
GGTCATAAGC ATGTACCTGA TTGGGTCTGG ATCGTTGTGG GCATCCTCAA CTTCGTTGCC 
TACACTCTAG ATGGAGTAGA TGGAAAACAA GCTCGTAGAA CTAATTCCAG CACCCCATTA 
GGGGAGCTGT TTGACCACGG CCTGGACAGT TGGGCATGCG TTTATTTCGT TGTGACTGTG 
TACTCTATCT TTGGACGAGG ACCGACTGGC GTCAGTGTTT TTGTTCTTTA CCTCCTGCTG 
TGGGTGGTTT TGTTTTCTTT TATCCTGTCT CACTGGGAGA AATATAACAC AGGCGTTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAA GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGATGTGCA TTGTGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTATA GAAGCTATAA AAGCAACTCT CTGAAGCACA ATTCCATCTA TGAAGCCATG 
GTCCCCTTCT TCTCTCCATG CCTGCTCTTC ACTCTGTCGA CAGTGTGGAT CCTCCGGTCA 
CCTTCAGATA TTTTAGAAAT GCACCCTAGA ATATTCTACT TCATGGTCGG AACAGCTTTT 
GCCAATATCA CATGTCAGCT AATCGTTTGC CAAATGAGCA GCACACGGTG CCCAACTTTG 
AACTGGTTGC TGCTTCCTCT CTTCTTGGTC GTGGCAACTG TGATCGTAGG CACAACAACC 
TCCCGCCTTG AGAGTGTTCT CCTTTACACA CTCACTGCTG CCTTCACTCT GGCTCACATC 
CATTATGGAG TACAAGTGGT AAAGCAGCTG AGCCGACATT TTCAGATTTA TCCTTTTTCA 
TTGAGGAAAC CAAACTCAGA TTGACTAGGA CTGGAAGAGA AGAGTATCGG TCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128226.2
CDS	98..1294
Translation	MAGYEYVSPEQLAGFDKYKYSALDTNPLSLYVMHPFWNTIVKVFPTWLAPNLITFSGFMLLVFNFLLLTYFDPDFYASAPGHKHVPDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWACVYFVVTVYSIFGRGPTGVSVFVLYLLLWVVLFSFILSHWEKYNTGVLFLPWGYDISQVTISFVYIVTAVVGVEAWYEPFLFNFLYRDLFTAMIIGCALCVTLPMSLLNFYRSYKSNSLKHNSIYEAMVPFFSPCLLFTLSTVWILRSPSDILEMHPRIFYFMVGTAFANITCQLIVCQMSSTRCPTLNWLLLPLFLVVATVIVGTTTSRLESVLLYTLTAAFTLAHIHYGVQVVKQLSRHFQIYPFSLRKPNSD*LGLEEKSIGP

Target ORF information:

RefSeq Version	NM_001134754.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus selenoprotein I (Selenoi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134754.1

ORF Insert Sequence:

ATGGCTGGCT ACGAATACGT GAGCCCAGAG CAGCTGGCGG GCTTTGACAA GTATAAGTAC 
AGCGCTTTGG ATACCAACCC ACTCTCTCTG TATGTCATGC ACCCATTTTG GAACACTATA 
GTGAAGGTAT TTCCCACTTG GCTGGCTCCC AATCTTATAA CCTTTTCTGG CTTTATGCTG 
CTTGTGTTCA ATTTCCTACT CCTGACATAC TTCGACCCTG ACTTCTATGC TTCAGCGCCA 
GGTCATAAGC ATGTACCTGA TTGGGTCTGG ATCGTTGTGG GCATCCTCAA CTTCGTTGCC 
TACACTCTAG ATGGAGTAGA TGGAAAACAA GCTCGTAGAA CTAATTCCAG CACCCCATTA 
GGGGAGCTGT TTGACCACGG CCTGGACAGT TGGGCATGCG TTTATTTCGT TGTGACTGTG 
TACTCTATCT TTGGACGAGG ACCGACTGGC GTCAGTGTTT TTGTTCTTTA CCTCCTGCTG 
TGGGTGGTTT TGTTTTCTTT TATCCTGTCT CACTGGGAGA AATATAACAC AGGCGTTCTT 
TTCCTGCCAT GGGGATATGA CATTAGCCAA GTGACTATTT CTTTTGTCTA CATAGTGACT 
GCGGTTGTGG GAGTTGAGGC CTGGTATGAA CCTTTCCTGT TTAATTTCTT ATATAGAGAC 
CTATTCACTG CAATGATTAT TGGATGTGCA TTGTGTGTGA CTCTTCCAAT GAGTTTATTA 
AACTTTTATA GAAGCTATAA AAGCAACTCT CTGAAGCACA ATTCCATCTA TGAAGCCATG 
GTCCCCTTCT TCTCTCCATG CCTGCTCTTC ACTCTGTCGA CAGTGTGGAT CCTCCGGTCA 
CCTTCAGATA TTTTAGAAAT GCACCCTAGA ATATTCTACT TCATGGTCGG AACAGCTTTT 
GCCAATATCA CATGTCAGCT AATCGTTTGC CAAATGAGCA GCACACGGTG CCCAACTTTG 
AACTGGTTGC TGCTTCCTCT CTTCTTGGTC GTGGCAACTG TGATCGTAGG CACAACAACC 
TCCCGCCTTG AGAGTGTTCT CCTTTACACA CTCACTGCTG CCTTCACTCT GGCTCACATC 
CATTATGGAG TACAAGTGGT AAAGCAGCTG AGCCGACATT TTCAGATTTA TCCTTTTTCA 
TTGAGGAAAC CAAACTCAGA TTGACTAGGA CTGGAAGAGA AGAGTATCGG TCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Identification and characterization of human ethanolaminephosphotransferase1.
Journal of lipid research48(3)503-8(2007 Mar)
Horibata Y,Hirabayashi Y

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

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Selenoi cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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