gRNAs
The following Mios gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mios cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06603 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017592684.1 , NM_001173554.1 , XM_006236090.3 | |
| Accession Version | NM_001173554.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | WD repeat-containing protein mio | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07059762.1. On or before May 13, 2010 this sequence version replaced XM_342642.4, XM_001055421.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001167025.1 |
| CDS | 311..2938 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001173554.1 |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06603 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017592684.1 , NM_001173554.1 , XM_006236090.3 | |
| Accession Version | XM_017592684.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | WD repeat-containing protein mio isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017448173.1 |
| CDS | 2476..5103 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017592684.1 |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06603 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017592684.1 , NM_001173554.1 , XM_006236090.3 | |
| Accession Version | XM_006236090.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | WD repeat-containing protein mio isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006236152.1 |
| CDS | 322..2949 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006236090.3 |
1 | ATGAGCGGTA CCAAGCCTGA TATTTTGTGG GCACCACACC AGGTTGATAG ATTCGTTGTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1. |
Regulation of mTORC1 by amino acids. |
A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. |
Integral and associated lysosomal membrane proteins. |
