gRNAs
The following Ift52 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift52 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06662 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001177685.1 | |
| Accession Version | NM_001177685.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | intraflagellar transport protein 52 homolog isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC162007.1. On or before May 13, 2010 this sequence version replaced XM_002729251.2, XM_002726291.1. Transcript Variant: This variant (2) uses an alternate splice junction in the 3' end of the coding sequence compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC162007.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGAAGG AGCTGAGGAG TACCATTCTT TTCAATGCAT ACAAAAAGGA GGTGTTTACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001171156.1 |
| CDS | 60..1172 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001177685.1 |
1 | ATGGAGAAGG AGCTGAGGAG TACCATTCTT TTCAATGCAT ACAAAAAGGA GGTGTTTACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa48174 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001308389.1 | |
| Accession Version | NM_001308389.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | intraflagellar transport protein 52 homolog isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07054547.1 and BC162007.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGAAGG AGCTGAGGAG TACCATTCTT TTCAATGCAT ACAAAAAGGA GGTGTTTACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001295318.1 |
| CDS | 60..1340 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001308389.1 |
1 | ATGGAGAAGG AGCTGAGGAG TACCATTCTT TTCAATGCAT ACAAAAAGGA GGTGTTTACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
IFT52 mutations destabilize anterograde complex assembly, disrupt ciliogenesis and result in short rib polydactyly syndrome. |
The intraflagellar transport protein IFT27 promotes BBSome exit from cilia through the GTPase ARL6/BBS3. |
C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. |
TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella. |
