Map1lc3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map1lc3a
Entrez Gene ID	362245
Full Name	microtubule-associated protein 1 light chain 3 alpha
Synonyms	LC3-I,LC3-II,LC3A
General protein information	Preferred Names microtubule-associated protein 1 light chain 3 alpha Names microtubule-associated proteins 1A/1B light chain 3A MAP1 light chain 3-like protein 1 MAP1A/1B light chain 3 A MAP1A/MAP1B LC3 A MAP1A/MAP1B light chain 3 A autophagy-related protein LC3 A autophagy-related ubiquitin-like modifier LC3 A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	light chain subunit of MAP1A and MAP1B microtubule-associated proteins; mediates interactions between microtubules and the cytoskeleton [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199500.2	NP_955794.1	microtubule-associated proteins 1A/1B light chain 3A

Pathways from BioSystems

Homologies

	MAP1LC3A	XP_001104172.1
Caenorhabditis elegans (roundworm)	lgg-2	NP_001255523.1
Rattus norvegicus (Norway rat)	Map1lc3a	NP_955794.1
Gallus gallus (chicken)	MAP1LC3A	XP_417327.2
Danio rerio (zebrafish)	map1lc3a	NP_999904.1
Homo sapiens (human)	MAP1LC3A	NP_115903.1
Canis lupus familiaris (dog)	MAP1LC3A	XP_863854.1
Pan troglodytes (chimpanzee)	MAP1LC3A	XP_001159668.1
Bos taurus (cattle)	MAP1LC3A	NP_001039640.1
Mus musculus (house mouse)	Map1lc3a	NP_080011.1
Xenopus tropicalis (tropical clawed frog)	map1lc3a	NP_001007979.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.
Redmann M, Benavides GA, Berryhill TF, Wani WY, Ouyang X, Johnson MS, Ravi S, Barnes S, Darley-Usmar VM, Zhang J
Redox biology1173-81(2017 04)

Human CLEC16A regulates autophagy through modulating mTOR activity.
Tam RC, Li MW, Gao YP, Pang YT, Yan S, Ge W, Lau CS, Chan VS
Experimental cell research352(2)304-312(2017 03)

Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth.
Cheng Z, Zhu Q, Dee R, Opheim Z, Mack CP, Cyr DM, Taylor JM
The Journal of biological chemistry292(6)2065-2079(2017 02)

MicroRNA-30b Regulates High Phosphorus Level-Induced Autophagy in Vascular Smooth Muscle Cells by Targeting BECN1.
Wang J, Sun YT, Xu TH, Sun W, Tian BY, Sheng ZT, Sun L, Liu LL, Ma JF, Wang LN, Yao L
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(2)530-536(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that both bafilomycin and chloroquine could significantly increase the autophagosome marker LC3-II and inhibit key parameters of mitochondrial function, and increase mtDNA damage.
Title: Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.

treatment with an autophagy inhibitor decreased LC3II expression.However, miR-30b overexpression decreased Pi-induced expression of autophagy-related marker genes such as BECN1, ATG5, and LC3b, whereas miR-30b downregulation increased Pi-induced expression of these genes. In addition, we found that miR-30b
Title: MicroRNA-30b Regulates High Phosphorus Level-Induced Autophagy in Vascular Smooth Muscle Cells by Targeting BECN1.

autophagy of cytoplasmic bulk cargo was completely LC3 independent.
Title: Autophagy of cytoplasmic bulk cargo does not require LC3.

LC3B modulates Angiotensin II-induced cardiomyocyte hypertrophy in cultured ventricular cardiomyocytes.
Title: LC3B, a Protein That Serves as an Autophagic Marker, Modulates Angiotensin II-induced Myocardial Hypertrophy.

Our findings indicate that increased autophagy in the cerebellum of dogs naturally infected with CDV may play a role in transferring the virus from cell to cell.
Title: Immunohistochemical detection of autophagy-related microtubule-associated protein 1 light chain 3 (LC3) in the cerebellums of dogs naturally infected with canine distemper virus.

The following Map1lc3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map1lc3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05392	NM_199500.2 Latest version!	Rattus norvegicus microtubule-associated protein 1 light chain 3 alpha (Map1lc3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map1lc3a ( NM_199500.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955794.1 Rattus norvegicus microtubule-associated protein 1 light chain 3 alpha (Map1lc3a), mRNA.

Price & Availability↑

CloneID	ORa05392
Clone ID Related Accession (Same CDS sequence)	NM_199500.2
Accession Version	NM_199500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	microtubule-associated proteins 1A/1B light chain 3A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM063664.1, FM046244.1, FM036735.1 and FM053631.1. On Feb 22, 2012 this sequence version replaced NM_199500.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY206668.1, BC086389.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTCCG ACCGGCCTTT CAAGCAGCGG CGCAGCTTCG CCGACCGCTG TAAGGAGGTG 
CAGCAGATCC GTGACCAGCA CCCCAGCAAG ATCCCGGTGA TCATCGAGCG CTACAAGGGT 
GAGAAGCAGC TGCCTGTCCT GGATAAGACC AAGTTTCTGG TCCCAGACCA TGTTAACATG 
AGCGAGTTGG TCAAGATCAT CCGGCGCCGC CTGCAACTCA ACCCCACGCA GGCCTTCTTC 
CTGCTGGTGA ACCAGCACAG CATGGTGAGT GTATCCACAC CCATCGCTGA CATCTATGAA 
CAGGAGAAGG ATGAAGACGG ATTCCTCTAC ATGGTCTATG CCTCCCAAGA AACCTTCGGC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955794.1
CDS	176..541
Translation	MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF

Target ORF information:

RefSeq Version	NM_199500.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microtubule-associated protein 1 light chain 3 alpha (Map1lc3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199500.2

ORF Insert Sequence:

ATGCCCTCCG ACCGGCCTTT CAAGCAGCGG CGCAGCTTCG CCGACCGCTG TAAGGAGGTG 
CAGCAGATCC GTGACCAGCA CCCCAGCAAG ATCCCGGTGA TCATCGAGCG CTACAAGGGT 
GAGAAGCAGC TGCCTGTCCT GGATAAGACC AAGTTTCTGG TCCCAGACCA TGTTAACATG 
AGCGAGTTGG TCAAGATCAT CCGGCGCCGC CTGCAACTCA ACCCCACGCA GGCCTTCTTC 
CTGCTGGTGA ACCAGCACAG CATGGTGAGT GTATCCACAC CCATCGCTGA CATCTATGAA 
CAGGAGAAGG ATGAAGACGG ATTCCTCTAC ATGGTCTATG CCTCCCAAGA AACCTTCGGC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.
Redox biology1173-81(2017 04)
Redmann M,Benavides GA,Berryhill TF,Wani WY,Ouyang X,Johnson MS,Ravi S,Barnes S,Darley-Usmar VM,Zhang J

Human CLEC16A regulates autophagy through modulating mTOR activity.
Experimental cell research352(2)304-312(2017 03)
Tam RC,Li MW,Gao YP,Pang YT,Yan S,Ge W,Lau CS,Chan VS

Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth.
The Journal of biological chemistry292(6)2065-2079(2017 02)
Cheng Z,Zhu Q,Dee R,Opheim Z,Mack CP,Cyr DM,Taylor JM

MicroRNA-30b Regulates High Phosphorus Level-Induced Autophagy in Vascular Smooth Muscle Cells by Targeting BECN1.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology42(2)530-536(2017)
Wang J,Sun YT,Xu TH,Sun W,Tian BY,Sheng ZT,Sun L,Liu LL,Ma JF,Wang LN,Yao L

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Map1lc3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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