Clca2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clca2
Entrez Gene ID	362052
Full Name	chloride channel calcium activated 2
Synonyms	CLCA,Clca4,rbCLCA
General protein information	Preferred Names chloride channel calcium activated 2 Names chloride channel calcium activated 2 Ca(2+)-activated chloride channel splicing calcium activated chloride channel chloride channel calcium activated 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44
Summary	calcium-sensitive chloride channel which is expressed in endothelial cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013202.2	NP_001013220.1	chloride channel calcium activated 2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC784768	NP_001229512.1
Danio rerio (zebrafish)	clca1	XP_002664610.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004059	XP_560062.4
Xenopus tropicalis (tropical clawed frog)	LOC100495107	XP_002931721.2
Canis lupus familiaris (dog)	LOC479964	XP_005622181.1
Bos taurus (cattle)	CLCA3	NP_851361.1
Mus musculus (house mouse)	Clca2	NP_085104.1
Rattus norvegicus (Norway rat)	Clca2	NP_001013220.1
Xenopus tropicalis (tropical clawed frog)	clca1	NP_001184129.1
Xenopus tropicalis (tropical clawed frog)	LOC100490932	XP_002934201.1
	LOC715292	XP_001105446.1
Mus musculus (house mouse)	Clca1	NP_034029.2
Xenopus tropicalis (tropical clawed frog)	LOC100488301	XP_002934186.1
Gallus gallus (chicken)	LOC424523	XP_422360.3
Xenopus tropicalis (tropical clawed frog)	clca3p	XP_002934204.1

Gene Ontology
Bibliography

Related articles in PubMed

CLCA splicing isoform associated with adhesion through ?1-integrin and its scaffolding protein: specific expression in undifferentiated epithelial cells.
Yamazaki J, Okamura K, Uehara K, Hatta M
The Journal of biological chemistry288(7)4831-43(2013 Feb)

Characterization of CLCA protein expressed in ductal cells of rat salivary glands.
Yamazaki J, Okamura K, Ishibashi K, Kitamura K
Biochimica et biophysica acta1715(2)132-44(2005 Sep)

Cloning and expression of Ca2+-activated chloride channel from rat brain.
Jeong SM, Park HK, Yoon IS, Lee JH, Kim JH, Jang CG, Lee CJ, Nah SY
Biochemical and biophysical research communications334(2)569-76(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CLCA splicing isoform is associated with adhesion through beta1-integrin and its scaffolding protein
Title: CLCA splicing isoform associated with adhesion through ?1-integrin and its scaffolding protein: specific expression in undifferentiated epithelial cells.

We successfully cloned a rat brain CLCA (rbCLCA).
Title: Cloning and expression of Ca2+-activated chloride channel from rat brain.

The following Clca2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clca2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03800	NM_001013202.2 Latest version!	Rattus norvegicus chloride channel calcium activated 2 (Clca2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clca2 ( NM_001013202.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013220.1 Rattus norvegicus chloride channel calcium activated 2 (Clca2), mRNA.

Price & Availability↑

CloneID	ORa03800
Clone ID Related Accession (Same CDS sequence)	NM_001013202.2
Accession Version	NM_001013202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	chloride channel calcium activated 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB119249.1. On Aug 30, 2012 this sequence version replaced NM_001013202.1. ##Evidence-Data-START## Transcript exon combination :: AB119249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCCAG TGCTGAAGGT CCTTCTGTTC CTCACCCTGC ACCTCTTGCA GGACACAAAA 
AGCTTCAAGG TCCATCTCAA CAACAATGGA TATGAGGGTG TGGTCATTGC AATTAACCCC 
AGTGTACCAG AGGATGAAAG ACTCATCCCA AGCTTAAAGG AAATGGTCAC TCAAGCCTCT 
ACTTACCTGT TTGAAGCCAG CCAAGGAAGG TTTTACTTCA GGAATGTAAG CATATTAGTC 
CCGATGACCT GGAAGTCAAA ATCTGAGTAC TTGATGCCAA AACGAGAATC ATATGACAAA 
GCAGATGTCA TAGTTGCCAA TTCTCATCTG AAATATGGAG ACAATCCCTA CACCCTTCAA 
TATGGGCAGT GTGGGGACAG AGGACGGTAC ATACATTTCA CTCCAAACTT CCTGCTCACT 
GATAACGTGC GTAACTATGG ACCCCGAGGC AGAGTCTTTG TCCATGAGTG GGCTCATCTC 
CGGTGGGGAG TGTTTGATGA GTATAATGAG GACCGGCCTT TCTACATATC TGGAAAGAAC 
ACTATCGAAG TGACAAGGTG TTCTACCGAC ATCAAGGGCT CTAAGGCGGT CCATGAGCGC 
CAGAGAGGCA GCGATGTGAC AAGGTTATGC CGATGGGACT CGAGGACAGG GCTGTATGAA 
CCCAAATGTA AATTTTTCCC AGACAAAATT CAGACTGCCA GGGCCTCCAT AATGTTCATG 
CAAAATCTCA ATTCCGTGGT TGAATTTTGC ACAGAAAAAA CCCATAATAC GGAAGCCCCA 
AACCTACAAA ACAAAATATG CAATGGCAGA AGCACGTGGG ACGTAATCAA GGAGTCCGCT 
GACTTTCAGC AGGCCCCTCC CATGAGAGGA ACAGAAGCCC CTCCTCCACC CACGTTTTCC 
TTGCTCAAGT CCAGACAGCG AGTGATCTGC TTGGTGCTGG ATAAATCCGG AAGCATGGAC 
ACAGAAGACC GCCTTATTCG AATGAATCAA GCAGCGGAAC TGTACTTAAC TCAAATTGTT 
GAAAAGGAAT CTATGGTTGG ATTAGTCACT TTTGACAGCA CTGCCCAAAT CCAAAATTAC 
CTGATAAAAA TAACTAATAC CGGTGACTAC AAGAAAATCA CTGGAAATCT CCCTCAGCAG 
GCTGTTGGGG GGACTTCAAT CTGCAGGGGA CTGGAAGCAG GATTTCAGGC AATTACCTCC 
AGTGACCAGA GTACTTCCGG TTCTGAGATT GTATTGCTAA CAGATGGGGA AGATGATCTA 
ATAAGTTCAT GCTTTGAGGT GGTCAAACAC AGCGGTGCGG TCATTCACAC CATCGCCCTG 
GGGCCTAAAG CTGCCCGAGA ACTGGAGACT TTGTCGGATA TGACAGGAGG GCTTCGCTTT 
TATGCCAACA AAGATGTGAA CAGCCTGATG GACGCTTTCA GTGGGATTTC CTCTGCAAGT 
GGCAACCTCT CCCAGCAGGC TCTGCAGTTG GAGAGCAAAG CCTTCAATAT TGGAGGGGGG 
ACATGGATAA ATGGTACAGT ACCTGTGGAC AGTACCATTG GCAACGACAC TTTCTTTGTT 
ATTACCTGGA CAGTCCAAAA GCCAGAAATT ATTCTTCGAG ATCCAAAAAG AAAAAAATAT 
ACAACCTCAG ATTTCCAAGA CAATAAACTA AACATTCGAT CTGCTCGACT TCAAATACCA 
GGCACTGCAG AGACAGGAAC TTGGACTTAC AGCCTCTTCA ATAAGGGGGT CAAACCTCAG 
TTGCTAACTG TGACAGTGAC CACTCGAGCC AGAAGTCCCA CCACACAGCC CCTCATGGCC 
ACCGCTCACA TGAGTCAGAG CACAGCCCAG TACCCTAGTC CGATGATTGT GTATGCACGG 
GTCAGCCAAG GGTTTTTGCC TGTTCTGGGA GCCAATGTCA CAGCCGTCAT AGAATCTGAA 
AATGGAAGTC GAGTCACCCT GGAGCTCTGG GACAACGGGG CAGGTGCTGA CACTGTCAAA 
AATGATGGCA TCTACACGAG ATATTTTACA GATTATCACG GAAATGGTAG ATACAGCTTA 
AAAGTGCGTG TCCAGGCAAG AAAGAGCAAA GCCAGACTAA GTTTAAGACA AAAGAACAAG 
TCTTTATATA TACCTGGCTA TGTTGAAAAT GGTAAAATTA TACTGAATCC ACCCAGACCG 
GAAGTCCCAG AAGAAGCTAC AGAAGCTGCA GTGGAAGACT TCAGCAGACT AACCTCTGGA 
GAGTCGTTTA CTGTGTCCGG AGCGCCCCCT GATGGCGGCC ACGCTCATGT GTTCCCACCC 
AGTAAAGTTA CAGACCTGGA GGCCGAGTTT AAAGGTGATC ATATTCACCT TACCTGGACG 
GCCCCTGGCA AGGTTCTCGA TAAAGGAAGA GCGCACAGAT ACGTCATCAG AATGAGCCGG 
CATCCTCCTG GTCTCCAAGA TTTTAACGAC GCTACTTTAG TGAATACTTC CGGTCTGATA 
CCTAAAGAGG CTGGCTCAGA AGAAACGTTT AAATTCAAAC CAGCAAGTTT TAAAATAGAA 
AACGGCACCC AGCTCTACAT TGCAATTCTG GCCAACAATG AAGCCGGTCT CACCTCTGAG 
GTCTCCAACA TCGCACAGGC TGTCAACTTT ATTCCTCCAG AACATAGTTT CTCTACACTC 
AGCCCTGATA TTTCTGCAAT CTGTCTGACA ATTTGGGGGT TAACTGTGAT TCTTAACGAT 
ATTTTAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013220.1
CDS	20..2731
Translation	MVPVLKVLLFLTLHLLQDTKSFKVHLNNNGYEGVVIAINPSVPEDERLIPSLKEMVTQASTYLFEASQGRFYFRNVSILVPMTWKSKSEYLMPKRESYDKADVIVANSHLKYGDNPYTLQYGQCGDRGRYIHFTPNFLLTDNVRNYGPRGRVFVHEWAHLRWGVFDEYNEDRPFYISGKNTIEVTRCSTDIKGSKAVHERQRGSDVTRLCRWDSRTGLYEPKCKFFPDKIQTARASIMFMQNLNSVVEFCTEKTHNTEAPNLQNKICNGRSTWDVIKESADFQQAPPMRGTEAPPPPTFSLLKSRQRVICLVLDKSGSMDTEDRLIRMNQAAELYLTQIVEKESMVGLVTFDSTAQIQNYLIKITNTGDYKKITGNLPQQAVGGTSICRGLEAGFQAITSSDQSTSGSEIVLLTDGEDDLISSCFEVVKHSGAVIHTIALGPKAARELETLSDMTGGLRFYANKDVNSLMDAFSGISSASGNLSQQALQLESKAFNIGGGTWINGTVPVDSTIGNDTFFVITWTVQKPEIILRDPKRKKYTTSDFQDNKLNIRSARLQIPGTAETGTWTYSLFNKGVKPQLLTVTVTTRARSPTTQPLMATAHMSQSTAQYPSPMIVYARVSQGFLPVLGANVTAVIESENGSRVTLELWDNGAGADTVKNDGIYTRYFTDYHGNGRYSLKVRVQARKSKARLSLRQKNKSLYIPGYVENGKIILNPPRPEVPEEATEAAVEDFSRLTSGESFTVSGAPPDGGHAHVFPPSKVTDLEAEFKGDHIHLTWTAPGKVLDKGRAHRYVIRMSRHPPGLQDFNDATLVNTSGLIPKEAGSEETFKFKPASFKIENGTQLYIAILANNEAGLTSEVSNIAQAVNFIPPEHSFSTLSPDISAICLTIWGLTVILNDILN

Target ORF information:

RefSeq Version	NM_001013202.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus chloride channel calcium activated 2 (Clca2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013202.2

ORF Insert Sequence:

ATGGTGCCAG TGCTGAAGGT CCTTCTGTTC CTCACCCTGC ACCTCTTGCA GGACACAAAA 
AGCTTCAAGG TCCATCTCAA CAACAATGGA TATGAGGGTG TGGTCATTGC AATTAACCCC 
AGTGTACCAG AGGATGAAAG ACTCATCCCA AGCTTAAAGG AAATGGTCAC TCAAGCCTCT 
ACTTACCTGT TTGAAGCCAG CCAAGGAAGG TTTTACTTCA GGAATGTAAG CATATTAGTC 
CCGATGACCT GGAAGTCAAA ATCTGAGTAC TTGATGCCAA AACGAGAATC ATATGACAAA 
GCAGATGTCA TAGTTGCCAA TTCTCATCTG AAATATGGAG ACAATCCCTA CACCCTTCAA 
TATGGGCAGT GTGGGGACAG AGGACGGTAC ATACATTTCA CTCCAAACTT CCTGCTCACT 
GATAACGTGC GTAACTATGG ACCCCGAGGC AGAGTCTTTG TCCATGAGTG GGCTCATCTC 
CGGTGGGGAG TGTTTGATGA GTATAATGAG GACCGGCCTT TCTACATATC TGGAAAGAAC 
ACTATCGAAG TGACAAGGTG TTCTACCGAC ATCAAGGGCT CTAAGGCGGT CCATGAGCGC 
CAGAGAGGCA GCGATGTGAC AAGGTTATGC CGATGGGACT CGAGGACAGG GCTGTATGAA 
CCCAAATGTA AATTTTTCCC AGACAAAATT CAGACTGCCA GGGCCTCCAT AATGTTCATG 
CAAAATCTCA ATTCCGTGGT TGAATTTTGC ACAGAAAAAA CCCATAATAC GGAAGCCCCA 
AACCTACAAA ACAAAATATG CAATGGCAGA AGCACGTGGG ACGTAATCAA GGAGTCCGCT 
GACTTTCAGC AGGCCCCTCC CATGAGAGGA ACAGAAGCCC CTCCTCCACC CACGTTTTCC 
TTGCTCAAGT CCAGACAGCG AGTGATCTGC TTGGTGCTGG ATAAATCCGG AAGCATGGAC 
ACAGAAGACC GCCTTATTCG AATGAATCAA GCAGCGGAAC TGTACTTAAC TCAAATTGTT 
GAAAAGGAAT CTATGGTTGG ATTAGTCACT TTTGACAGCA CTGCCCAAAT CCAAAATTAC 
CTGATAAAAA TAACTAATAC CGGTGACTAC AAGAAAATCA CTGGAAATCT CCCTCAGCAG 
GCTGTTGGGG GGACTTCAAT CTGCAGGGGA CTGGAAGCAG GATTTCAGGC AATTACCTCC 
AGTGACCAGA GTACTTCCGG TTCTGAGATT GTATTGCTAA CAGATGGGGA AGATGATCTA 
ATAAGTTCAT GCTTTGAGGT GGTCAAACAC AGCGGTGCGG TCATTCACAC CATCGCCCTG 
GGGCCTAAAG CTGCCCGAGA ACTGGAGACT TTGTCGGATA TGACAGGAGG GCTTCGCTTT 
TATGCCAACA AAGATGTGAA CAGCCTGATG GACGCTTTCA GTGGGATTTC CTCTGCAAGT 
GGCAACCTCT CCCAGCAGGC TCTGCAGTTG GAGAGCAAAG CCTTCAATAT TGGAGGGGGG 
ACATGGATAA ATGGTACAGT ACCTGTGGAC AGTACCATTG GCAACGACAC TTTCTTTGTT 
ATTACCTGGA CAGTCCAAAA GCCAGAAATT ATTCTTCGAG ATCCAAAAAG AAAAAAATAT 
ACAACCTCAG ATTTCCAAGA CAATAAACTA AACATTCGAT CTGCTCGACT TCAAATACCA 
GGCACTGCAG AGACAGGAAC TTGGACTTAC AGCCTCTTCA ATAAGGGGGT CAAACCTCAG 
TTGCTAACTG TGACAGTGAC CACTCGAGCC AGAAGTCCCA CCACACAGCC CCTCATGGCC 
ACCGCTCACA TGAGTCAGAG CACAGCCCAG TACCCTAGTC CGATGATTGT GTATGCACGG 
GTCAGCCAAG GGTTTTTGCC TGTTCTGGGA GCCAATGTCA CAGCCGTCAT AGAATCTGAA 
AATGGAAGTC GAGTCACCCT GGAGCTCTGG GACAACGGGG CAGGTGCTGA CACTGTCAAA 
AATGATGGCA TCTACACGAG ATATTTTACA GATTATCACG GAAATGGTAG ATACAGCTTA 
AAAGTGCGTG TCCAGGCAAG AAAGAGCAAA GCCAGACTAA GTTTAAGACA AAAGAACAAG 
TCTTTATATA TACCTGGCTA TGTTGAAAAT GGTAAAATTA TACTGAATCC ACCCAGACCG 
GAAGTCCCAG AAGAAGCTAC AGAAGCTGCA GTGGAAGACT TCAGCAGACT AACCTCTGGA 
GAGTCGTTTA CTGTGTCCGG AGCGCCCCCT GATGGCGGCC ACGCTCATGT GTTCCCACCC 
AGTAAAGTTA CAGACCTGGA GGCCGAGTTT AAAGGTGATC ATATTCACCT TACCTGGACG 
GCCCCTGGCA AGGTTCTCGA TAAAGGAAGA GCGCACAGAT ACGTCATCAG AATGAGCCGG 
CATCCTCCTG GTCTCCAAGA TTTTAACGAC GCTACTTTAG TGAATACTTC CGGTCTGATA 
CCTAAAGAGG CTGGCTCAGA AGAAACGTTT AAATTCAAAC CAGCAAGTTT TAAAATAGAA 
AACGGCACCC AGCTCTACAT TGCAATTCTG GCCAACAATG AAGCCGGTCT CACCTCTGAG 
GTCTCCAACA TCGCACAGGC TGTCAACTTT ATTCCTCCAG AACATAGTTT CTCTACACTC 
AGCCCTGATA TTTCTGCAAT CTGTCTGACA ATTTGGGGGT TAACTGTGAT TCTTAACGAT 
ATTTTAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLCA splicing isoform associated with adhesion through ?1-integrin and its scaffolding protein: specific expression in undifferentiated epithelial cells.
The Journal of biological chemistry288(7)4831-43(2013 Feb)
Yamazaki J,Okamura K,Uehara K,Hatta M

Characterization of CLCA protein expressed in ductal cells of rat salivary glands.
Biochimica et biophysica acta1715(2)132-44(2005 Sep)
Yamazaki J,Okamura K,Ishibashi K,Kitamura K

Cloning and expression of Ca2+-activated chloride channel from rat brain.
Biochemical and biophysical research communications334(2)569-76(2005 Aug)
Jeong SM,Park HK,Yoon IS,Lee JH,Kim JH,Jang CG,Lee CJ,Nah SY

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Clca2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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