Ehmt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ehmt2
Entrez Gene ID	361798
Full Name	euchromatic histone lysine methyltransferase 2
Synonyms	Bat8,G9a,Ng36
General protein information	Preferred Names euchromatic histone lysine methyltransferase 2 Names histone-lysine N-methyltransferase EHMT2 HLA-B associated transcript 8, rat orthologue euchromatic histone-lysine N-methyltransferase 2 histone-lysine N-methyltransferase, H3 lysine-9 specific 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	mouse homolog may act as a histone methyltransferase and may be involved in genetic imprinting [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255957.2	XP_006256019.1	histone-lysine N-methyltransferase EHMT2 isoform X4
NM_212463.1	NP_997628.1	histone-lysine N-methyltransferase EHMT2
XM_006255955.3	XP_006256017.2	histone-lysine N-methyltransferase EHMT2 isoform X2
XM_006255956.3	XP_006256018.2	histone-lysine N-methyltransferase EHMT2 isoform X3
XM_006255959.2	XP_006256021.1	histone-lysine N-methyltransferase EHMT2 isoform X6
XM_006255954.3	XP_006256016.2	histone-lysine N-methyltransferase EHMT2 isoform X1
XM_006255958.2	XP_006256020.1	histone-lysine N-methyltransferase EHMT2 isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EHMT2	XP_518365.3
Canis lupus familiaris (dog)	EHMT2	XP_005627223.1
Rattus norvegicus (Norway rat)	Ehmt2	NP_997628.1
Danio rerio (zebrafish)	ehmt2	NP_001107087.1
Bos taurus (cattle)	EHMT2	NP_001193192.1
Mus musculus (house mouse)	Ehmt2	NP_665829.1
Homo sapiens (human)	EHMT2	NP_006700.3
	EHMT2	XP_001106224.2
Xenopus tropicalis (tropical clawed frog)	ehmt2	XP_004917185.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of the histone dimethyltransferase G9a in nucleus accumbens shell increases cocaine self-administration, stress-induced reinstatement, and anxiety.
Anderson EM, Larson EB, Guzman D, Marie Wissman A, Neve RL, Nestler EJ, Self DW
The Journal of neuroscience : the official journal of the Society for Neuroscience(2017 Dec)

Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
Sharma M, Dierkes T, Sajikumar S
Aging cell16(5)1062-1072(2017 Oct)

The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.
Thienpont B, Aronsen JM, Robinson EL, Okkenhaug H, Loche E, Ferrini A, Brien P, Alkass K, Tomasso A, Agrawal A, Bergmann O, Sjaastad I, Reik W, Roderick HL
The Journal of clinical investigation127(1)335-348(2017 Jan)

Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-mediated Transcriptional Repression of ?-Opioid Receptors in Primary Sensory Neurons.
Zhang Y, Chen SR, Laumet G, Chen H, Pan HL
The Journal of biological chemistry291(16)8475-85(2016 Apr)

H3K9 histone methyltransferase G9a ameliorates dilated cardiomyopathy via the downregulation of cell adhesion molecules.
Chen G, Wang X, Zhang Y, Ru X, Zhou L, Tian Y
Molecular medicine reports11(5)3872-9(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-217-mediated, genetic, or pharmacological inactivation of EHMT1/2 was sufficient to promote pathological hypertrophy
Title: The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.

findings indicate that G9a contributes critically to transcriptional repression of MORs in primary sensory neurons in neuropathic pain. G9a inhibitors may be used to enhance the opioid analgesic effect in the treatment of chronic neuropathic pain.
Title: Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-mediated Transcriptional Repression of ?-Opioid Receptors in Primary Sensory Neurons.

H3K9 histone methyltransferase G9a ameliorates dilated cardiomyopathy via the downregulation of cell adhesion molecules
Title: H3K9 histone methyltransferase G9a ameliorates dilated cardiomyopathy via the downregulation of cell adhesion molecules.

The histone methyltransferase G9a exhibited decreased protein levels and interactions with DNMT1 upon antidepressant exposure.
Title: Antidepressants inhibit DNA methyltransferase 1 through reducing G9a levels.

The data of this study demonstrated that G9a/GLP differentially regulates gene transcription in the hippocampus and the EC during memory consolidation
Title: G9a/GLP histone lysine dimethyltransferase complex activity in the hippocampus and the entorhinal cortex is required for gene activation and silencing during memory consolidation.

The following Ehmt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ehmt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05701	NM_212463.1 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$930.30 $1329.00
ORa39149	XM_006255957.2 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa39150	XM_006255958.2 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa39151	XM_006255959.2 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa48020	XM_006255954.3 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa48021	XM_006255955.3 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa48022	XM_006255956.3 Latest version!	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ehmt2 ( NM_212463.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_997628.1 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), mRNA.

Price & Availability↑

CloneID	ORa05701
Clone ID Related Accession (Same CDS sequence)	NM_212463.1
Accession Version	NM_212463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX883045.1. On Apr 19, 2005 this sequence version replaced XM_579740.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGAGTGAA TGGTGTGGGT TCCTCAGGGC CCAGTGAGTA CATGGAGGTT 
CCTCTGGGGT CCCTGGAGCT GCCCAGCGAG GGGACCCTCT CCCCCAACCA CGCTGGGGTC 
TCCAATGACA CGTCTTCGCT GGAGACGGAG CGTGGGTTTG AGGAGCTGCC CCTCTGCAGC 
TGCCGCATGG AGGCGCCCAA GATTGACCGC ATCAGCGAGA GAGCAGGACA CAAGTGTATG 
GCCACCGAAA GTGTGGACGG AGAGCTGCTG GGTTGCAATG CCGCCATCTT GAAGCGGGAG 
ACCATGCGGC CGTCTAGCCG TGTGGCGCTG ATGGTACTGT GTGAGGCCCA TCGAGCCCGC 
ATGGTCAAGC ACCATTGCTG CCCAGGCTGT GGCTACTTCT GCACGGCGGG CACCTTCCTG 
GAGTGTCACC CCGACTTCCG TGTAGCTCAC CGCTTCCATA AGGCCTGCGT GTCCCAGCTC 
AATGGGATGG TTTTCTGTCC CCACTGTGGG GAGGACGCCT CAGAGGCCCA GGAGGTGACC 
ATCCCTCGGG GCGATGGGGG AACACCCCCG GTTGGCACTG TGGCGCCTGC CCCGCCACCC 
CTGGCACACG ACGCCCCAGG GCGAGCGGAT ACCTCCCAGC CCAGTGCCCG AATGCGAGGA 
CACGGAGAGC CAAGGCGCCC ACCCTGTGAT CCCCTGGCCG ACACCATTGA CAGCTCGGGG 
CCTTCTCTGA CTCTGCCTAA TGGCGGCTGC CTCTCAGCTG TGGGTCTGCC CCCAGGGCCA 
GGCCGGGAAG CCCTGGAAAA AGCCTTGGTC ATCCAGGAGT CCGAGAGGAG GAAGAAGCTG 
CGGTTCCACC CGCGGCAGCT CTACCTGTCG GTGAAGCAGG GGGAGCTGCA GAAGGTGATC 
CTCATGCTGC TCGACAACCT GGACCCCAAC TTCCAGAGCG ACCAGCAGAG CAAGCGCACG 
CCCCTGCACG CAGCCGCCCA GAAGGGGTCC GTGGAGATCT GTCACGTGCT GCTACAGGCA 
GGAGCCAACA TCAACGCCGT GGATAAGCAG CAGCGCACCC CGCTGATGGA GGCCGTGGTG 
AACAACCACC TGGAGGTGGC TCGCTACATG GTGCAGCTGG GCGGCTGTGT GTACAGCAAG 
GAAGAGGATG GCTCTACCTG TCTCCATCAT GCGGCCAAAA TTGGGAACTT GGAAATGGTC 
AGCCTGCTGC TGAGCACAGG GCAGGTGGAC GTCAATGCCC AGGACAGTGG GGGCTGGACA 
CCCATCATCT GGGCAGCCGA GCACAAGCAC ATCGACGTGA TCCGTATGCT GCTGACCCGG 
GGCGCCGACG TCACCCTTAC CGACAACGAG GAAAACATCT GCCTGCACTG GGCTTCCTTC 
ACGGGTAGTG CCGCTATTGC CGAGGTCCTC CTGAATGCCC AGTGCGACCT CCATGCTGTC 
AACTACCACG GGGACACGCC CCTGCACATA GCCGCCAGGG AGAGCTACCA TGACTGCGTT 
CTGTTGTTCC TGTCCCGTGG AGCCAACCCT GAGCTTCGGA ACAAAGAAGG GGACACGGCG 
TGGGATCTGA CCCCAGAGCG CTCCGATGTG TGGTTTGCAC TGCAGCTCAA TCGCAAGCTT 
AGGCTCGGGG TAGGGAACCG GGCTGTCCGC ACCGAGAAGA TCATCTGCCG GGATGTAGCC 
CGAGGCTATG AGAATGTGCC CATTCCCTGT GTCAATGGCG TGGATGGGGA GCCATGCCCA 
GAGGACTATA AGTACATCTC CGAGAACTGT GAAACGTCCA CCATGAACAT CGACCGAAAC 
ATCACTCACC TGCAGCACTG CACGTGTGTT GACGACTGCT CCAGCTCCAA CTGCCTGTGT 
GGCCAGCTAA GCATCCGATG CTGGTATGAT AAGGACGGGC GGCTGCTCCA GGAGTTTAAC 
AAGATCGAGC CCCCCCTGAT CTTTGAGTGT AACCAGGCAT GCTCCTGCTG GAGAAGCTGC 
AAGAACCGCG TGGTGCAGAG CGGCATCAAG GTGCGGCTGC AGCTCTACCG GACGGCCAAG 
ATGGGCTGGG GGGTCCGTGC CTTGCAGACC ATTCCCCAGG GCACGTTCAT CTGCGAGTAC 
GTCGGGGAGC TGATCTCCGA CGCCGAGGCA GACGTGAGAG AGGATGATTC TTACCTCTTC 
GACTTAGACA ACAAGGATGG TGAGGTCTAC TGCATTGATG CCCGTTACTA TGGCAACATC 
AGCCGCTTCA TTAACCACCT GTGCGACCCC AACATCATCC CTGTTCGGGT CTTCATGCTG 
CATCAAGACC TACGGTTCCC TCGCATCGCC TTCTTCAGCT CCAGGGACAT CCGGACCGGG 
GAGGAGCTGG GGTTTGACTA CGGCGACCGG TTCTGGGATA TCAAGAGCAA GTATTTCACC 
TGCCAGTGTG GCTCTGAGAA GTGCAAGCAT TCAGCTGAGG CCATCGCCCT GGAGCAGAGC 
CGCCTGGCCC GTCTGGACCC CCACCCGGAG CTGCTCCCTG ACCTCAGCTC CCTGCCCCCC 
ATCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997628.1
CDS	1..3792
Translation	MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPSQGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	NM_212463.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212463.1

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGAGTGAA TGGTGTGGGT TCCTCAGGGC CCAGTGAGTA CATGGAGGTT 
CCTCTGGGGT CCCTGGAGCT GCCCAGCGAG GGGACCCTCT CCCCCAACCA CGCTGGGGTC 
TCCAATGACA CGTCTTCGCT GGAGACGGAG CGTGGGTTTG AGGAGCTGCC CCTCTGCAGC 
TGCCGCATGG AGGCGCCCAA GATTGACCGC ATCAGCGAGA GAGCAGGACA CAAGTGTATG 
GCCACCGAAA GTGTGGACGG AGAGCTGCTG GGTTGCAATG CCGCCATCTT GAAGCGGGAG 
ACCATGCGGC CGTCTAGCCG TGTGGCGCTG ATGGTACTGT GTGAGGCCCA TCGAGCCCGC 
ATGGTCAAGC ACCATTGCTG CCCAGGCTGT GGCTACTTCT GCACGGCGGG CACCTTCCTG 
GAGTGTCACC CCGACTTCCG TGTAGCTCAC CGCTTCCATA AGGCCTGCGT GTCCCAGCTC 
AATGGGATGG TTTTCTGTCC CCACTGTGGG GAGGACGCCT CAGAGGCCCA GGAGGTGACC 
ATCCCTCGGG GCGATGGGGG AACACCCCCG GTTGGCACTG TGGCGCCTGC CCCGCCACCC 
CTGGCACACG ACGCCCCAGG GCGAGCGGAT ACCTCCCAGC CCAGTGCCCG AATGCGAGGA 
CACGGAGAGC CAAGGCGCCC ACCCTGTGAT CCCCTGGCCG ACACCATTGA CAGCTCGGGG 
CCTTCTCTGA CTCTGCCTAA TGGCGGCTGC CTCTCAGCTG TGGGTCTGCC CCCAGGGCCA 
GGCCGGGAAG CCCTGGAAAA AGCCTTGGTC ATCCAGGAGT CCGAGAGGAG GAAGAAGCTG 
CGGTTCCACC CGCGGCAGCT CTACCTGTCG GTGAAGCAGG GGGAGCTGCA GAAGGTGATC 
CTCATGCTGC TCGACAACCT GGACCCCAAC TTCCAGAGCG ACCAGCAGAG CAAGCGCACG 
CCCCTGCACG CAGCCGCCCA GAAGGGGTCC GTGGAGATCT GTCACGTGCT GCTACAGGCA 
GGAGCCAACA TCAACGCCGT GGATAAGCAG CAGCGCACCC CGCTGATGGA GGCCGTGGTG 
AACAACCACC TGGAGGTGGC TCGCTACATG GTGCAGCTGG GCGGCTGTGT GTACAGCAAG 
GAAGAGGATG GCTCTACCTG TCTCCATCAT GCGGCCAAAA TTGGGAACTT GGAAATGGTC 
AGCCTGCTGC TGAGCACAGG GCAGGTGGAC GTCAATGCCC AGGACAGTGG GGGCTGGACA 
CCCATCATCT GGGCAGCCGA GCACAAGCAC ATCGACGTGA TCCGTATGCT GCTGACCCGG 
GGCGCCGACG TCACCCTTAC CGACAACGAG GAAAACATCT GCCTGCACTG GGCTTCCTTC 
ACGGGTAGTG CCGCTATTGC CGAGGTCCTC CTGAATGCCC AGTGCGACCT CCATGCTGTC 
AACTACCACG GGGACACGCC CCTGCACATA GCCGCCAGGG AGAGCTACCA TGACTGCGTT 
CTGTTGTTCC TGTCCCGTGG AGCCAACCCT GAGCTTCGGA ACAAAGAAGG GGACACGGCG 
TGGGATCTGA CCCCAGAGCG CTCCGATGTG TGGTTTGCAC TGCAGCTCAA TCGCAAGCTT 
AGGCTCGGGG TAGGGAACCG GGCTGTCCGC ACCGAGAAGA TCATCTGCCG GGATGTAGCC 
CGAGGCTATG AGAATGTGCC CATTCCCTGT GTCAATGGCG TGGATGGGGA GCCATGCCCA 
GAGGACTATA AGTACATCTC CGAGAACTGT GAAACGTCCA CCATGAACAT CGACCGAAAC 
ATCACTCACC TGCAGCACTG CACGTGTGTT GACGACTGCT CCAGCTCCAA CTGCCTGTGT 
GGCCAGCTAA GCATCCGATG CTGGTATGAT AAGGACGGGC GGCTGCTCCA GGAGTTTAAC 
AAGATCGAGC CCCCCCTGAT CTTTGAGTGT AACCAGGCAT GCTCCTGCTG GAGAAGCTGC 
AAGAACCGCG TGGTGCAGAG CGGCATCAAG GTGCGGCTGC AGCTCTACCG GACGGCCAAG 
ATGGGCTGGG GGGTCCGTGC CTTGCAGACC ATTCCCCAGG GCACGTTCAT CTGCGAGTAC 
GTCGGGGAGC TGATCTCCGA CGCCGAGGCA GACGTGAGAG AGGATGATTC TTACCTCTTC 
GACTTAGACA ACAAGGATGG TGAGGTCTAC TGCATTGATG CCCGTTACTA TGGCAACATC 
AGCCGCTTCA TTAACCACCT GTGCGACCCC AACATCATCC CTGTTCGGGT CTTCATGCTG 
CATCAAGACC TACGGTTCCC TCGCATCGCC TTCTTCAGCT CCAGGGACAT CCGGACCGGG 
GAGGAGCTGG GGTTTGACTA CGGCGACCGG TTCTGGGATA TCAAGAGCAA GTATTTCACC 
TGCCAGTGTG GCTCTGAGAA GTGCAAGCAT TCAGCTGAGG CCATCGCCCT GGAGCAGAGC 
CGCCTGGCCC GTCTGGACCC CCACCCGGAG CTGCTCCCTG ACCTCAGCTC CCTGCCCCCC 
ATCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255957.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256019.1 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa39149
Clone ID Related Accession (Same CDS sequence)	XM_006255957.2
Accession Version	XM_006255957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGAGTGAAT GGTGTGGGTT CCTCAGGGCC CAGTGAGTAC 
ATGGAGGTTC CTCTGGGGTC CCTGGAGCTG CCCAGCGAGG GGACCCTCTC CCCCAACCAC 
GCTGGGGTCT CCAATGACAC GTCTTCGCTG GAGACGGAGC GTGGGTTTGA GGAGCTGCCC 
CTCTGCAGCT GCCGCATGGA GGCGCCCAAG ATTGACCGCA TCAGCGAGAG AGCAGGACAC 
AAGTGTATGG CCACCGAAAG TGTGGACGGA GAGCTGCTGG GTTGCAATGC CGCCATCTTG 
AAGCGGGAGA CCATGCGGCC GTCTAGCCGT GTGGCGCTGA TGGTACTGTG TGAGGCCCAT 
CGAGCCCGCA TGGTCAAGCA CCATTGCTGC CCAGGCTGTG GCTACTTCTG CACGGCGGGC 
ACCTTCCTGG AGTGTCACCC CGACTTCCGT GTAGCTCACC GCTTCCATAA GGCCTGCGTG 
TCCCAGCTCA ATGGGATGGT TTTCTGTCCC CACTGTGGGG AGGACGCCTC AGAGGCCCAG 
GAGGTGACCA TCCCTCGGGG CGATGGGGGA ACACCCCCGG TTGGCACTGT GGCGCCTGCC 
CCGCCACCCC TGGCACACGA CGCCCCAGGG CGAGCGGATA CCTCCCAGCC CAGTGCCCGA 
ATGCGAGGAC ACGGAGAGCC AAGGCGCCCA CCCTGTGATC CCCTGGCCGA CACCATTGAC 
AGCTCGGGGC CTTCTCTGAC TCTGCCTAAT GGCGGCTGCC TCTCAGCTGT GGGTCTGCCC 
CCAGGGCCAG GCCGGGAAGC CCTGGAAAAA GCCTTGGTCA TCCAGGAGTC CGAGAGCCTA 
CCTTCTCCCC CTCCGTCCCC GGGCAGGAGG AAGAAGCTGC GGTTCCACCC GCGGCAGCTC 
TACCTGTCGG TGAAGCAGGG GGAGCTGCAG AAGGTGATCC TCATGCTGCT CGACAACCTG 
GACCCCAACT TCCAGAGCGA CCAGCAGAGC AAGCGCACGC CCCTGCACGC AGCCGCCCAG 
AAGGGGTCCG TGGAGATCTG TCACGTGCTG CTACAGGCAG GAGCCAACAT CAACGCCGTG 
GATAAGCAGC AGCGCACCCC GCTGATGGAG GCCGTGGTGA ACAACCACCT GGAGGTGGCT 
CGCTACATGG TGCAGCTGGG CGGCTGTGTG TACAGCAAGG AAGAGGATGG CTCTACCTGT 
CTCCATCATG CGGCCAAAAT TGGGAACTTG GAAATGGTCA GCCTGCTGCT GAGCACAGGG 
CAGGTGGACG TCAATGCCCA GGACAGTGGG GGCTGGACAC CCATCATCTG GGCAGCCGAG 
CACAAGCACA TCGACGTGAT CCGTATGCTG CTGACCCGGG GCGCCGACGT CACCCTTACC 
GACAACGAGG AAAACATCTG CCTGCACTGG GCTTCCTTCA CGGGTAGTGC CGCTATTGCC 
GAGGTCCTCC TGAATGCCCA GTGCGACCTC CATGCTGTCA ACTACCACGG GGACACGCCC 
CTGCACATAG CCGCCAGGGA GAGCTACCAT GACTGCGTTC TGTTGTTCCT GTCCCGTGGA 
GCCAACCCTG AGCTTCGGAA CAAAGAAGGG GACACGGCGT GGGATCTGAC CCCAGAGCGC 
TCCGATGTGT GGTTTGCACT GCAGCTCAAT CGCAAGCTTA GGCTCGGGGT AGGGAACCGG 
GCTGTCCGCA CCGAGAAGAT CATCTGCCGG GATGTAGCCC GAGGCTATGA GAATGTGCCC 
ATTCCCTGTG TCAATGGCGT GGATGGGGAG CCATGCCCAG AGGACTATAA GTACATCTCC 
GAGAACTGTG AAACGTCCAC CATGAACATC GACCGAAACA TCACTCACCT GCAGCACTGC 
ACGTGTGTTG ACGACTGCTC CAGCTCCAAC TGCCTGTGTG GCCAGCTAAG CATCCGATGC 
TGGTATGATA AGGACGGGCG GCTGCTCCAG GAGTTTAACA AGATCGAGCC CCCCCTGATC 
TTTGAGTGTA ACCAGGCATG CTCCTGCTGG AGAAGCTGCA AGAACCGCGT GGTGCAGAGC 
GGCATCAAGG TGCGGCTGCA GCTCTACCGG ACGGCCAAGA TGGGCTGGGG GGTCCGTGCC 
TTGCAGACCA TTCCCCAGGG CACGTTCATC TGCGAGTACG TCGGGGAGCT GATCTCCGAC 
GCCGAGGCAG ACGTGAGAGA GGATGATTCT TACCTCTTCG ACTTAGACAA CAAGGATGGT 
GAGGTCTACT GCATTGATGC CCGTTACTAT GGCAACATCA GCCGCTTCAT TAACCACCTG 
TGCGACCCCA ACATCATCCC TGTTCGGGTC TTCATGCTGC ATCAAGACCT ACGGTTCCCT 
CGCATCGCCT TCTTCAGCTC CAGGGACATC CGGACCGGGG AGGAGCTGGG GTTTGACTAC 
GGCGACCGGT TCTGGGATAT CAAGAGCAAG TATTTCACCT GCCAGTGTGG CTCTGAGAAG 
TGCAAGCATT CAGCTGAGGC CATCGCCCTG GAGCAGAGCC GCCTGGCCCG TCTGGACCCC 
CACCCGGAGC TGCTCCCTGA CCTCAGCTCC CTGCCCCCCA TCAACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256019.1
CDS	30..3680
Translation	MAAAAGAAAAAAAEGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESESLPSPPPSPGRRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255957.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255957.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGAGTGAAT GGTGTGGGTT CCTCAGGGCC CAGTGAGTAC 
ATGGAGGTTC CTCTGGGGTC CCTGGAGCTG CCCAGCGAGG GGACCCTCTC CCCCAACCAC 
GCTGGGGTCT CCAATGACAC GTCTTCGCTG GAGACGGAGC GTGGGTTTGA GGAGCTGCCC 
CTCTGCAGCT GCCGCATGGA GGCGCCCAAG ATTGACCGCA TCAGCGAGAG AGCAGGACAC 
AAGTGTATGG CCACCGAAAG TGTGGACGGA GAGCTGCTGG GTTGCAATGC CGCCATCTTG 
AAGCGGGAGA CCATGCGGCC GTCTAGCCGT GTGGCGCTGA TGGTACTGTG TGAGGCCCAT 
CGAGCCCGCA TGGTCAAGCA CCATTGCTGC CCAGGCTGTG GCTACTTCTG CACGGCGGGC 
ACCTTCCTGG AGTGTCACCC CGACTTCCGT GTAGCTCACC GCTTCCATAA GGCCTGCGTG 
TCCCAGCTCA ATGGGATGGT TTTCTGTCCC CACTGTGGGG AGGACGCCTC AGAGGCCCAG 
GAGGTGACCA TCCCTCGGGG CGATGGGGGA ACACCCCCGG TTGGCACTGT GGCGCCTGCC 
CCGCCACCCC TGGCACACGA CGCCCCAGGG CGAGCGGATA CCTCCCAGCC CAGTGCCCGA 
ATGCGAGGAC ACGGAGAGCC AAGGCGCCCA CCCTGTGATC CCCTGGCCGA CACCATTGAC 
AGCTCGGGGC CTTCTCTGAC TCTGCCTAAT GGCGGCTGCC TCTCAGCTGT GGGTCTGCCC 
CCAGGGCCAG GCCGGGAAGC CCTGGAAAAA GCCTTGGTCA TCCAGGAGTC CGAGAGCCTA 
CCTTCTCCCC CTCCGTCCCC GGGCAGGAGG AAGAAGCTGC GGTTCCACCC GCGGCAGCTC 
TACCTGTCGG TGAAGCAGGG GGAGCTGCAG AAGGTGATCC TCATGCTGCT CGACAACCTG 
GACCCCAACT TCCAGAGCGA CCAGCAGAGC AAGCGCACGC CCCTGCACGC AGCCGCCCAG 
AAGGGGTCCG TGGAGATCTG TCACGTGCTG CTACAGGCAG GAGCCAACAT CAACGCCGTG 
GATAAGCAGC AGCGCACCCC GCTGATGGAG GCCGTGGTGA ACAACCACCT GGAGGTGGCT 
CGCTACATGG TGCAGCTGGG CGGCTGTGTG TACAGCAAGG AAGAGGATGG CTCTACCTGT 
CTCCATCATG CGGCCAAAAT TGGGAACTTG GAAATGGTCA GCCTGCTGCT GAGCACAGGG 
CAGGTGGACG TCAATGCCCA GGACAGTGGG GGCTGGACAC CCATCATCTG GGCAGCCGAG 
CACAAGCACA TCGACGTGAT CCGTATGCTG CTGACCCGGG GCGCCGACGT CACCCTTACC 
GACAACGAGG AAAACATCTG CCTGCACTGG GCTTCCTTCA CGGGTAGTGC CGCTATTGCC 
GAGGTCCTCC TGAATGCCCA GTGCGACCTC CATGCTGTCA ACTACCACGG GGACACGCCC 
CTGCACATAG CCGCCAGGGA GAGCTACCAT GACTGCGTTC TGTTGTTCCT GTCCCGTGGA 
GCCAACCCTG AGCTTCGGAA CAAAGAAGGG GACACGGCGT GGGATCTGAC CCCAGAGCGC 
TCCGATGTGT GGTTTGCACT GCAGCTCAAT CGCAAGCTTA GGCTCGGGGT AGGGAACCGG 
GCTGTCCGCA CCGAGAAGAT CATCTGCCGG GATGTAGCCC GAGGCTATGA GAATGTGCCC 
ATTCCCTGTG TCAATGGCGT GGATGGGGAG CCATGCCCAG AGGACTATAA GTACATCTCC 
GAGAACTGTG AAACGTCCAC CATGAACATC GACCGAAACA TCACTCACCT GCAGCACTGC 
ACGTGTGTTG ACGACTGCTC CAGCTCCAAC TGCCTGTGTG GCCAGCTAAG CATCCGATGC 
TGGTATGATA AGGACGGGCG GCTGCTCCAG GAGTTTAACA AGATCGAGCC CCCCCTGATC 
TTTGAGTGTA ACCAGGCATG CTCCTGCTGG AGAAGCTGCA AGAACCGCGT GGTGCAGAGC 
GGCATCAAGG TGCGGCTGCA GCTCTACCGG ACGGCCAAGA TGGGCTGGGG GGTCCGTGCC 
TTGCAGACCA TTCCCCAGGG CACGTTCATC TGCGAGTACG TCGGGGAGCT GATCTCCGAC 
GCCGAGGCAG ACGTGAGAGA GGATGATTCT TACCTCTTCG ACTTAGACAA CAAGGATGGT 
GAGGTCTACT GCATTGATGC CCGTTACTAT GGCAACATCA GCCGCTTCAT TAACCACCTG 
TGCGACCCCA ACATCATCCC TGTTCGGGTC TTCATGCTGC ATCAAGACCT ACGGTTCCCT 
CGCATCGCCT TCTTCAGCTC CAGGGACATC CGGACCGGGG AGGAGCTGGG GTTTGACTAC 
GGCGACCGGT TCTGGGATAT CAAGAGCAAG TATTTCACCT GCCAGTGTGG CTCTGAGAAG 
TGCAAGCATT CAGCTGAGGC CATCGCCCTG GAGCAGAGCC GCCTGGCCCG TCTGGACCCC 
CACCCGGAGC TGCTCCCTGA CCTCAGCTCC CTGCCCCCCA TCAACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255958.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256020.1 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X5, mRNA.

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CloneID	ORa39150
Clone ID Related Accession (Same CDS sequence)	XM_006255958.2
Accession Version	XM_006255958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGAGTGAAT GGTGTGGGTT CCTCAGGGCC CAGTGAGTAC 
ATGGAGGTTC CTCTGGGGTC CCTGGAGCTG CCCAGCGAGG GGACCCTCTC CCCCAACCAC 
GCTGGGGTCT CCAATGACAC GTCTTCGCTG GAGACGGAGC GTGGGTTTGA GGAGCTGCCC 
CTCTGCAGCT GCCGCATGGA GGCGCCCAAG ATTGACCGCA TCAGCGAGAG AGCAGGACAC 
AAGTGTATGG CCACCGAAAG TGTGGACGGA GAGCTGCTGG GTTGCAATGC CGCCATCTTG 
AAGCGGGAGA CCATGCGGCC GTCTAGCCGT GTGGCGCTGA TGGTACTGTG TGAGGCCCAT 
CGAGCCCGCA TGGTCAAGCA CCATTGCTGC CCAGGCTGTG GCTACTTCTG CACGGCGGGC 
ACCTTCCTGG AGTGTCACCC CGACTTCCGT GTAGCTCACC GCTTCCATAA GGCCTGCGTG 
TCCCAGCTCA ATGGGATGGT TTTCTGTCCC CACTGTGGGG AGGACGCCTC AGAGGCCCAG 
GAGGTGACCA TCCCTCGGGG CGATGGGGGA ACACCCCCGG TTGGCACTGT GGCGCCTGCC 
CCGCCACCCC TGGCACACGA CGCCCCAGGG CGAGCGGATA CCTCCCAGCC CAGTGCCCGA 
ATGCGAGGAC ACGGAGAGCC AAGGCGCCCA CCCTGTGATC CCCTGGCCGA CACCATTGAC 
AGCTCGGGGC CTTCTCTGAC TCTGCCTAAT GGCGGCTGCC TCTCAGCTGT GGGTCTGCCC 
CCAGGGCCAG GCCGGGAAGC CCTGGAAAAA GCCTTGGTCA TCCAGGAGTC CGAGAGGAGG 
AAGAAGCTGC GGTTCCACCC GCGGCAGCTC TACCTGTCGG TGAAGCAGGG GGAGCTGCAG 
AAGGTGATCC TCATGCTGCT CGACAACCTG GACCCCAACT TCCAGAGCGA CCAGCAGAGC 
AAGCGCACGC CCCTGCACGC AGCCGCCCAG AAGGGGTCCG TGGAGATCTG TCACGTGCTG 
CTACAGGCAG GAGCCAACAT CAACGCCGTG GATAAGCAGC AGCGCACCCC GCTGATGGAG 
GCCGTGGTGA ACAACCACCT GGAGGTGGCT CGCTACATGG TGCAGCTGGG CGGCTGTGTG 
TACAGCAAGG AAGAGGATGG CTCTACCTGT CTCCATCATG CGGCCAAAAT TGGGAACTTG 
GAAATGGTCA GCCTGCTGCT GAGCACAGGG CAGGTGGACG TCAATGCCCA GGACAGTGGG 
GGCTGGACAC CCATCATCTG GGCAGCCGAG CACAAGCACA TCGACGTGAT CCGTATGCTG 
CTGACCCGGG GCGCCGACGT CACCCTTACC GACAACGAGG AAAACATCTG CCTGCACTGG 
GCTTCCTTCA CGGGTAGTGC CGCTATTGCC GAGGTCCTCC TGAATGCCCA GTGCGACCTC 
CATGCTGTCA ACTACCACGG GGACACGCCC CTGCACATAG CCGCCAGGGA GAGCTACCAT 
GACTGCGTTC TGTTGTTCCT GTCCCGTGGA GCCAACCCTG AGCTTCGGAA CAAAGAAGGG 
GACACGGCGT GGGATCTGAC CCCAGAGCGC TCCGATGTGT GGTTTGCACT GCAGCTCAAT 
CGCAAGCTTA GGCTCGGGGT AGGGAACCGG GCTGTCCGCA CCGAGAAGAT CATCTGCCGG 
GATGTAGCCC GAGGCTATGA GAATGTGCCC ATTCCCTGTG TCAATGGCGT GGATGGGGAG 
CCATGCCCAG AGGACTATAA GTACATCTCC GAGAACTGTG AAACGTCCAC CATGAACATC 
GACCGAAACA TCACTCACCT GCAGCACTGC ACGTGTGTTG ACGACTGCTC CAGCTCCAAC 
TGCCTGTGTG GCCAGCTAAG CATCCGATGC TGGTATGATA AGGACGGGCG GCTGCTCCAG 
GAGTTTAACA AGATCGAGCC CCCCCTGATC TTTGAGTGTA ACCAGGCATG CTCCTGCTGG 
AGAAGCTGCA AGAACCGCGT GGTGCAGAGC GGCATCAAGG TGCGGCTGCA GCTCTACCGG 
ACGGCCAAGA TGGGCTGGGG GGTCCGTGCC TTGCAGACCA TTCCCCAGGG CACGTTCATC 
TGCGAGTACG TCGGGGAGCT GATCTCCGAC GCCGAGGCAG ACGTGAGAGA GGATGATTCT 
TACCTCTTCG ACTTAGACAA CAAGGATGGT GAGGTCTACT GCATTGATGC CCGTTACTAT 
GGCAACATCA GCCGCTTCAT TAACCACCTG TGCGACCCCA ACATCATCCC TGTTCGGGTC 
TTCATGCTGC ATCAAGACCT ACGGTTCCCT CGCATCGCCT TCTTCAGCTC CAGGGACATC 
CGGACCGGGG AGGAGCTGGG GTTTGACTAC GGCGACCGGT TCTGGGATAT CAAGAGCAAG 
TATTTCACCT GCCAGTGTGG CTCTGAGAAG TGCAAGCATT CAGCTGAGGC CATCGCCCTG 
GAGCAGAGCC GCCTGGCCCG TCTGGACCCC CACCCGGAGC TGCTCCCTGA CCTCAGCTCC 
CTGCCCCCCA TCAACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256020.1
CDS	30..3650
Translation	MAAAAGAAAAAAAEGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255958.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255958.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGAGTGAAT GGTGTGGGTT CCTCAGGGCC CAGTGAGTAC 
ATGGAGGTTC CTCTGGGGTC CCTGGAGCTG CCCAGCGAGG GGACCCTCTC CCCCAACCAC 
GCTGGGGTCT CCAATGACAC GTCTTCGCTG GAGACGGAGC GTGGGTTTGA GGAGCTGCCC 
CTCTGCAGCT GCCGCATGGA GGCGCCCAAG ATTGACCGCA TCAGCGAGAG AGCAGGACAC 
AAGTGTATGG CCACCGAAAG TGTGGACGGA GAGCTGCTGG GTTGCAATGC CGCCATCTTG 
AAGCGGGAGA CCATGCGGCC GTCTAGCCGT GTGGCGCTGA TGGTACTGTG TGAGGCCCAT 
CGAGCCCGCA TGGTCAAGCA CCATTGCTGC CCAGGCTGTG GCTACTTCTG CACGGCGGGC 
ACCTTCCTGG AGTGTCACCC CGACTTCCGT GTAGCTCACC GCTTCCATAA GGCCTGCGTG 
TCCCAGCTCA ATGGGATGGT TTTCTGTCCC CACTGTGGGG AGGACGCCTC AGAGGCCCAG 
GAGGTGACCA TCCCTCGGGG CGATGGGGGA ACACCCCCGG TTGGCACTGT GGCGCCTGCC 
CCGCCACCCC TGGCACACGA CGCCCCAGGG CGAGCGGATA CCTCCCAGCC CAGTGCCCGA 
ATGCGAGGAC ACGGAGAGCC AAGGCGCCCA CCCTGTGATC CCCTGGCCGA CACCATTGAC 
AGCTCGGGGC CTTCTCTGAC TCTGCCTAAT GGCGGCTGCC TCTCAGCTGT GGGTCTGCCC 
CCAGGGCCAG GCCGGGAAGC CCTGGAAAAA GCCTTGGTCA TCCAGGAGTC CGAGAGGAGG 
AAGAAGCTGC GGTTCCACCC GCGGCAGCTC TACCTGTCGG TGAAGCAGGG GGAGCTGCAG 
AAGGTGATCC TCATGCTGCT CGACAACCTG GACCCCAACT TCCAGAGCGA CCAGCAGAGC 
AAGCGCACGC CCCTGCACGC AGCCGCCCAG AAGGGGTCCG TGGAGATCTG TCACGTGCTG 
CTACAGGCAG GAGCCAACAT CAACGCCGTG GATAAGCAGC AGCGCACCCC GCTGATGGAG 
GCCGTGGTGA ACAACCACCT GGAGGTGGCT CGCTACATGG TGCAGCTGGG CGGCTGTGTG 
TACAGCAAGG AAGAGGATGG CTCTACCTGT CTCCATCATG CGGCCAAAAT TGGGAACTTG 
GAAATGGTCA GCCTGCTGCT GAGCACAGGG CAGGTGGACG TCAATGCCCA GGACAGTGGG 
GGCTGGACAC CCATCATCTG GGCAGCCGAG CACAAGCACA TCGACGTGAT CCGTATGCTG 
CTGACCCGGG GCGCCGACGT CACCCTTACC GACAACGAGG AAAACATCTG CCTGCACTGG 
GCTTCCTTCA CGGGTAGTGC CGCTATTGCC GAGGTCCTCC TGAATGCCCA GTGCGACCTC 
CATGCTGTCA ACTACCACGG GGACACGCCC CTGCACATAG CCGCCAGGGA GAGCTACCAT 
GACTGCGTTC TGTTGTTCCT GTCCCGTGGA GCCAACCCTG AGCTTCGGAA CAAAGAAGGG 
GACACGGCGT GGGATCTGAC CCCAGAGCGC TCCGATGTGT GGTTTGCACT GCAGCTCAAT 
CGCAAGCTTA GGCTCGGGGT AGGGAACCGG GCTGTCCGCA CCGAGAAGAT CATCTGCCGG 
GATGTAGCCC GAGGCTATGA GAATGTGCCC ATTCCCTGTG TCAATGGCGT GGATGGGGAG 
CCATGCCCAG AGGACTATAA GTACATCTCC GAGAACTGTG AAACGTCCAC CATGAACATC 
GACCGAAACA TCACTCACCT GCAGCACTGC ACGTGTGTTG ACGACTGCTC CAGCTCCAAC 
TGCCTGTGTG GCCAGCTAAG CATCCGATGC TGGTATGATA AGGACGGGCG GCTGCTCCAG 
GAGTTTAACA AGATCGAGCC CCCCCTGATC TTTGAGTGTA ACCAGGCATG CTCCTGCTGG 
AGAAGCTGCA AGAACCGCGT GGTGCAGAGC GGCATCAAGG TGCGGCTGCA GCTCTACCGG 
ACGGCCAAGA TGGGCTGGGG GGTCCGTGCC TTGCAGACCA TTCCCCAGGG CACGTTCATC 
TGCGAGTACG TCGGGGAGCT GATCTCCGAC GCCGAGGCAG ACGTGAGAGA GGATGATTCT 
TACCTCTTCG ACTTAGACAA CAAGGATGGT GAGGTCTACT GCATTGATGC CCGTTACTAT 
GGCAACATCA GCCGCTTCAT TAACCACCTG TGCGACCCCA ACATCATCCC TGTTCGGGTC 
TTCATGCTGC ATCAAGACCT ACGGTTCCCT CGCATCGCCT TCTTCAGCTC CAGGGACATC 
CGGACCGGGG AGGAGCTGGG GTTTGACTAC GGCGACCGGT TCTGGGATAT CAAGAGCAAG 
TATTTCACCT GCCAGTGTGG CTCTGAGAAG TGCAAGCATT CAGCTGAGGC CATCGCCCTG 
GAGCAGAGCC GCCTGGCCCG TCTGGACCCC CACCCGGAGC TGCTCCCTGA CCTCAGCTCC 
CTGCCCCCCA TCAACACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255959.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256021.1 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X6, mRNA.

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CloneID	ORa39151
Clone ID Related Accession (Same CDS sequence)	XM_006255959.2
Accession Version	XM_006255959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGGGTCTCC AATGACACGT CTTCGCTGGA GACGGAGCGT 
GGGTTTGAGG AGCTGCCCCT CTGCAGCTGC CGCATGGAGG CGCCCAAGAT TGACCGCATC 
AGCGAGAGAG CAGGACACAA GTGTATGGCC ACCGAAAGTG TGGACGGAGA GCTGCTGGGT 
TGCAATGCCG CCATCTTGAA GCGGGAGACC ATGCGGCCGT CTAGCCGTGT GGCGCTGATG 
GTACTGTGTG AGGCCCATCG AGCCCGCATG GTCAAGCACC ATTGCTGCCC AGGCTGTGGC 
TACTTCTGCA CGGCGGGCAC CTTCCTGGAG TGTCACCCCG ACTTCCGTGT AGCTCACCGC 
TTCCATAAGG CCTGCGTGTC CCAGCTCAAT GGGATGGTTT TCTGTCCCCA CTGTGGGGAG 
GACGCCTCAG AGGCCCAGGA GGTGACCATC CCTCGGGGCG ATGGGGGAAC ACCCCCGGTT 
GGCACTGTGG CGCCTGCCCC GCCACCCCTG GCACACGACG CCCCAGGGCG AGCGGATACC 
TCCCAGCCCA GTGCCCGAAT GCGAGGACAC GGAGAGCCAA GGCGCCCACC CTGTGATCCC 
CTGGCCGACA CCATTGACAG CTCGGGGCCT TCTCTGACTC TGCCTAATGG CGGCTGCCTC 
TCAGCTGTGG GTCTGCCCCC AGGGCCAGGC CGGGAAGCCC TGGAAAAAGC CTTGGTCATC 
CAGGAGTCCG AGAGGAGGAA GAAGCTGCGG TTCCACCCGC GGCAGCTCTA CCTGTCGGTG 
AAGCAGGGGG AGCTGCAGAA GGTGATCCTC ATGCTGCTCG ACAACCTGGA CCCCAACTTC 
CAGAGCGACC AGCAGAGCAA GCGCACGCCC CTGCACGCAG CCGCCCAGAA GGGGTCCGTG 
GAGATCTGTC ACGTGCTGCT ACAGGCAGGA GCCAACATCA ACGCCGTGGA TAAGCAGCAG 
CGCACCCCGC TGATGGAGGC CGTGGTGAAC AACCACCTGG AGGTGGCTCG CTACATGGTG 
CAGCTGGGCG GCTGTGTGTA CAGCAAGGAA GAGGATGGCT CTACCTGTCT CCATCATGCG 
GCCAAAATTG GGAACTTGGA AATGGTCAGC CTGCTGCTGA GCACAGGGCA GGTGGACGTC 
AATGCCCAGG ACAGTGGGGG CTGGACACCC ATCATCTGGG CAGCCGAGCA CAAGCACATC 
GACGTGATCC GTATGCTGCT GACCCGGGGC GCCGACGTCA CCCTTACCGA CAACGAGGAA 
AACATCTGCC TGCACTGGGC TTCCTTCACG GGTAGTGCCG CTATTGCCGA GGTCCTCCTG 
AATGCCCAGT GCGACCTCCA TGCTGTCAAC TACCACGGGG ACACGCCCCT GCACATAGCC 
GCCAGGGAGA GCTACCATGA CTGCGTTCTG TTGTTCCTGT CCCGTGGAGC CAACCCTGAG 
CTTCGGAACA AAGAAGGGGA CACGGCGTGG GATCTGACCC CAGAGCGCTC CGATGTGTGG 
TTTGCACTGC AGCTCAATCG CAAGCTTAGG CTCGGGGTAG GGAACCGGGC TGTCCGCACC 
GAGAAGATCA TCTGCCGGGA TGTAGCCCGA GGCTATGAGA ATGTGCCCAT TCCCTGTGTC 
AATGGCGTGG ATGGGGAGCC ATGCCCAGAG GACTATAAGT ACATCTCCGA GAACTGTGAA 
ACGTCCACCA TGAACATCGA CCGAAACATC ACTCACCTGC AGCACTGCAC GTGTGTTGAC 
GACTGCTCCA GCTCCAACTG CCTGTGTGGC CAGCTAAGCA TCCGATGCTG GTATGATAAG 
GACGGGCGGC TGCTCCAGGA GTTTAACAAG ATCGAGCCCC CCCTGATCTT TGAGTGTAAC 
CAGGCATGCT CCTGCTGGAG AAGCTGCAAG AACCGCGTGG TGCAGAGCGG CATCAAGGTG 
CGGCTGCAGC TCTACCGGAC GGCCAAGATG GGCTGGGGGG TCCGTGCCTT GCAGACCATT 
CCCCAGGGCA CGTTCATCTG CGAGTACGTC GGGGAGCTGA TCTCCGACGC CGAGGCAGAC 
GTGAGAGAGG ATGATTCTTA CCTCTTCGAC TTAGACAACA AGGATGGTGA GGTCTACTGC 
ATTGATGCCC GTTACTATGG CAACATCAGC CGCTTCATTA ACCACCTGTG CGACCCCAAC 
ATCATCCCTG TTCGGGTCTT CATGCTGCAT CAAGACCTAC GGTTCCCTCG CATCGCCTTC 
TTCAGCTCCA GGGACATCCG GACCGGGGAG GAGCTGGGGT TTGACTACGG CGACCGGTTC 
TGGGATATCA AGAGCAAGTA TTTCACCTGC CAGTGTGGCT CTGAGAAGTG CAAGCATTCA 
GCTGAGGCCA TCGCCCTGGA GCAGAGCCGC CTGGCCCGTC TGGACCCCCA CCCGGAGCTG 
CTCCCTGACC TCAGCTCCCT GCCCCCCATC AACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256021.1
CDS	30..3548
Translation	MAAAAGAAAAAAAEGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255959.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255959.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGGAGC TGCTGCGGCG GCGGCCGCCG AGGGGGAGGC CCCCGCTGAG 
ATGGGGGCGC TGCTGCTGGA GAAGGAGCCC CGAGGAGCCG CCGAGAGAGT TCATGGCTCT 
TTGGGGGACA CCTCTCATAG TGAGGAGACC CTTCCCAAGG CCAACCCCGA CTCCTTGGAG 
CCTACCGGCC CCTCCTCTCC GGCCTCTGTC ACTGTCACCG TCGGCGATGA GGGGGCTGAC 
ACCCCTGTCG GGGCCACACC GCTCATCGGG GAGGAACCCG AGAACCTGGA GGGAGATGGG 
GGTCGAATCC TGCTGGGCCA TGCCACAAAG TCATTCCCCT CTTCCCCCAG CAAGGGGGGT 
GCCTGTCCCA GTCGGGCCAA AATGTCAATG ACAGGGGCAG GAAAGTCGCC CCCCTCGGTC 
CAGAGTTTGG CCATGAGGCT GTTGAGCATG CCTGGGGCCC AGGGAGCTGC AACTGCTGGG 
CCTGAGCCCC CTCCGGCAAC AACTGCCGGC CAGGAGGGGC AGCCCAAAGT ACACCGAGCT 
CGGAAAACCA TGTCCAAGCC TAGCAACGGA CAGCCTCCAG TCCCTGAGAA GCGGCCCCCT 
GAAGTCCAGC ATTTCCGCAT GAGTGACGAC ATGCATCTGG GAAAGGTGAC TTCAGATGTG 
GCCAAACGGA GGAAGTTGAC CTCGGGCAGC CTGTCAGAGG ACTTGGGCTC TGCTGGGGGC 
TCAGGAGAAG TGATCCTGGA GAAGGGAGAG CCCAGGCCTC TGGAGGAGTG GGAGACGGTG 
GTGGGCGATG ACTTCAGCCT GTACTATGAC GCGTACTCTG TGGATGAGCG GGTGGACTCT 
GACAGCAAGT CTGAAGTCGA AGCTCTAGCT GAACAGTTGA GTGAAGAGGA GGAGGAGGAA 
GAGGAGGAAG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AGGAAGAGGA GGAGGAGGAT 
GAGGAGTCGG GCAACCAGTC AGACAGGAGT GGCTCTAGCG GCCGACGCAA GGCCAAGAAG 
AAATGGCGGA AAGACAGCCC ATGGGTGAAG CCATCCAGGA AACGGCGGAA GCGAGAGCCT 
CCGAGGGCCA AGGAGCCCAG AGGGGTCTCC AATGACACGT CTTCGCTGGA GACGGAGCGT 
GGGTTTGAGG AGCTGCCCCT CTGCAGCTGC CGCATGGAGG CGCCCAAGAT TGACCGCATC 
AGCGAGAGAG CAGGACACAA GTGTATGGCC ACCGAAAGTG TGGACGGAGA GCTGCTGGGT 
TGCAATGCCG CCATCTTGAA GCGGGAGACC ATGCGGCCGT CTAGCCGTGT GGCGCTGATG 
GTACTGTGTG AGGCCCATCG AGCCCGCATG GTCAAGCACC ATTGCTGCCC AGGCTGTGGC 
TACTTCTGCA CGGCGGGCAC CTTCCTGGAG TGTCACCCCG ACTTCCGTGT AGCTCACCGC 
TTCCATAAGG CCTGCGTGTC CCAGCTCAAT GGGATGGTTT TCTGTCCCCA CTGTGGGGAG 
GACGCCTCAG AGGCCCAGGA GGTGACCATC CCTCGGGGCG ATGGGGGAAC ACCCCCGGTT 
GGCACTGTGG CGCCTGCCCC GCCACCCCTG GCACACGACG CCCCAGGGCG AGCGGATACC 
TCCCAGCCCA GTGCCCGAAT GCGAGGACAC GGAGAGCCAA GGCGCCCACC CTGTGATCCC 
CTGGCCGACA CCATTGACAG CTCGGGGCCT TCTCTGACTC TGCCTAATGG CGGCTGCCTC 
TCAGCTGTGG GTCTGCCCCC AGGGCCAGGC CGGGAAGCCC TGGAAAAAGC CTTGGTCATC 
CAGGAGTCCG AGAGGAGGAA GAAGCTGCGG TTCCACCCGC GGCAGCTCTA CCTGTCGGTG 
AAGCAGGGGG AGCTGCAGAA GGTGATCCTC ATGCTGCTCG ACAACCTGGA CCCCAACTTC 
CAGAGCGACC AGCAGAGCAA GCGCACGCCC CTGCACGCAG CCGCCCAGAA GGGGTCCGTG 
GAGATCTGTC ACGTGCTGCT ACAGGCAGGA GCCAACATCA ACGCCGTGGA TAAGCAGCAG 
CGCACCCCGC TGATGGAGGC CGTGGTGAAC AACCACCTGG AGGTGGCTCG CTACATGGTG 
CAGCTGGGCG GCTGTGTGTA CAGCAAGGAA GAGGATGGCT CTACCTGTCT CCATCATGCG 
GCCAAAATTG GGAACTTGGA AATGGTCAGC CTGCTGCTGA GCACAGGGCA GGTGGACGTC 
AATGCCCAGG ACAGTGGGGG CTGGACACCC ATCATCTGGG CAGCCGAGCA CAAGCACATC 
GACGTGATCC GTATGCTGCT GACCCGGGGC GCCGACGTCA CCCTTACCGA CAACGAGGAA 
AACATCTGCC TGCACTGGGC TTCCTTCACG GGTAGTGCCG CTATTGCCGA GGTCCTCCTG 
AATGCCCAGT GCGACCTCCA TGCTGTCAAC TACCACGGGG ACACGCCCCT GCACATAGCC 
GCCAGGGAGA GCTACCATGA CTGCGTTCTG TTGTTCCTGT CCCGTGGAGC CAACCCTGAG 
CTTCGGAACA AAGAAGGGGA CACGGCGTGG GATCTGACCC CAGAGCGCTC CGATGTGTGG 
TTTGCACTGC AGCTCAATCG CAAGCTTAGG CTCGGGGTAG GGAACCGGGC TGTCCGCACC 
GAGAAGATCA TCTGCCGGGA TGTAGCCCGA GGCTATGAGA ATGTGCCCAT TCCCTGTGTC 
AATGGCGTGG ATGGGGAGCC ATGCCCAGAG GACTATAAGT ACATCTCCGA GAACTGTGAA 
ACGTCCACCA TGAACATCGA CCGAAACATC ACTCACCTGC AGCACTGCAC GTGTGTTGAC 
GACTGCTCCA GCTCCAACTG CCTGTGTGGC CAGCTAAGCA TCCGATGCTG GTATGATAAG 
GACGGGCGGC TGCTCCAGGA GTTTAACAAG ATCGAGCCCC CCCTGATCTT TGAGTGTAAC 
CAGGCATGCT CCTGCTGGAG AAGCTGCAAG AACCGCGTGG TGCAGAGCGG CATCAAGGTG 
CGGCTGCAGC TCTACCGGAC GGCCAAGATG GGCTGGGGGG TCCGTGCCTT GCAGACCATT 
CCCCAGGGCA CGTTCATCTG CGAGTACGTC GGGGAGCTGA TCTCCGACGC CGAGGCAGAC 
GTGAGAGAGG ATGATTCTTA CCTCTTCGAC TTAGACAACA AGGATGGTGA GGTCTACTGC 
ATTGATGCCC GTTACTATGG CAACATCAGC CGCTTCATTA ACCACCTGTG CGACCCCAAC 
ATCATCCCTG TTCGGGTCTT CATGCTGCAT CAAGACCTAC GGTTCCCTCG CATCGCCTTC 
TTCAGCTCCA GGGACATCCG GACCGGGGAG GAGCTGGGGT TTGACTACGG CGACCGGTTC 
TGGGATATCA AGAGCAAGTA TTTCACCTGC CAGTGTGGCT CTGAGAAGTG CAAGCATTCA 
GCTGAGGCCA TCGCCCTGGA GCAGAGCCGC CTGGCCCGTC TGGACCCCCA CCCGGAGCTG 
CTCCCTGACC TCAGCTCCCT GCCCCCCATC AACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255954.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256016.2 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X1, mRNA.

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CloneID	ORa48020
Clone ID Related Accession (Same CDS sequence)	XM_006255954.3
Accession Version	XM_006255954.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255954.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGAGTGAA TGGTGTGGGT TCCTCAGGGC CCAGTGAGTA CATGGAGGTT 
CCTCTGGGGT CCCTGGAGCT GCCCAGCGAG GGGACCCTCT CCCCCAACCA CGCTGGGGTC 
TCCAATGACA CGTCTTCGCT GGAGACGGAG CGTGGGTTTG AGGAGCTGCC CCTCTGCAGC 
TGCCGCATGG AGGCGCCCAA GATTGACCGC ATCAGCGAGA GAGCAGGACA CAAGTGTATG 
GCCACCGAAA GTGTGGACGG AGAGCTGCTG GGTTGCAATG CCGCCATCTT GAAGCGGGAG 
ACCATGCGGC CGTCTAGCCG TGTGGCGCTG ATGGTACTGT GTGAGGCCCA TCGAGCCCGC 
ATGGTCAAGC ACCATTGCTG CCCAGGCTGT GGCTACTTCT GCACGGCGGG CACCTTCCTG 
GAGTGTCACC CCGACTTCCG TGTAGCTCAC CGCTTCCATA AGGCCTGCGT GTCCCAGCTC 
AATGGGATGG TTTTCTGTCC CCACTGTGGG GAGGACGCCT CAGAGGCCCA GGAGGTGACC 
ATCCCTCGGG GCGATGGGGG AACACCCCCG GTTGGCACTG TGGCGCCTGC CCCGCCACCC 
CTGGCACACG ACGCCCCAGG GCGAGCGGAT ACCTCCCAGC CCAGTGCCCG AATGCGAGGA 
CACGGAGAGC CAAGGCGCCC ACCCTGTGAT CCCCTGGCCG ACACCATTGA CAGCTCGGGG 
CCTTCTCTGA CTCTGCCTAA TGGCGGCTGC CTCTCAGCTG TGGGTCTGCC CCCAGGGCCA 
GGCCGGGAAG CCCTGGAAAA AGCCTTGGTC ATCCAGGAGT CCGAGAGCCT ACCTTCTCCC 
CCTCCGTCCC CGGGCAGGAG GAAGAAGCTG CGGTTCCACC CGCGGCAGCT CTACCTGTCG 
GTGAAGCAGG GGGAGCTGCA GAAGGTGATC CTCATGCTGC TCGACAACCT GGACCCCAAC 
TTCCAGAGCG ACCAGCAGAG CAAGCGCACG CCCCTGCACG CAGCCGCCCA GAAGGGGTCC 
GTGGAGATCT GTCACGTGCT GCTACAGGCA GGAGCCAACA TCAACGCCGT GGATAAGCAG 
CAGCGCACCC CGCTGATGGA GGCCGTGGTG AACAACCACC TGGAGGTGGC TCGCTACATG 
GTGCAGCTGG GCGGCTGTGT GTACAGCAAG GAAGAGGATG GCTCTACCTG TCTCCATCAT 
GCGGCCAAAA TTGGGAACTT GGAAATGGTC AGCCTGCTGC TGAGCACAGG GCAGGTGGAC 
GTCAATGCCC AGGACAGTGG GGGCTGGACA CCCATCATCT GGGCAGCCGA GCACAAGCAC 
ATCGACGTGA TCCGTATGCT GCTGACCCGG GGCGCCGACG TCACCCTTAC CGACAACGAG 
GAAAACATCT GCCTGCACTG GGCTTCCTTC ACGGGTAGTG CCGCTATTGC CGAGGTCCTC 
CTGAATGCCC AGTGCGACCT CCATGCTGTC AACTACCACG GGGACACGCC CCTGCACATA 
GCCGCCAGGG AGAGCTACCA TGACTGCGTT CTGTTGTTCC TGTCCCGTGG AGCCAACCCT 
GAGCTTCGGA ACAAAGAAGG GGACACGGCG TGGGATCTGA CCCCAGAGCG CTCCGATGTG 
TGGTTTGCAC TGCAGCTCAA TCGCAAGCTT AGGCTCGGGG TAGGGAACCG GGCTGTCCGC 
ACCGAGAAGA TCATCTGCCG GGATGTAGCC CGAGGCTATG AGAATGTGCC CATTCCCTGT 
GTCAATGGCG TGGATGGGGA GCCATGCCCA GAGGACTATA AGTACATCTC CGAGAACTGT 
GAAACGTCCA CCATGAACAT CGACCGAAAC ATCACTCACC TGCAGCACTG CACGTGTGTT 
GACGACTGCT CCAGCTCCAA CTGCCTGTGT GGCCAGCTAA GCATCCGATG CTGGTATGAT 
AAGGACGGGC GGCTGCTCCA GGAGTTTAAC AAGATCGAGC CCCCCCTGAT CTTTGAGTGT 
AACCAGGCAT GCTCCTGCTG GAGAAGCTGC AAGAACCGCG TGGTGCAGAG CGGCATCAAG 
GTGCGGCTGC AGCTCTACCG GACGGCCAAG ATGGGCTGGG GGGTCCGTGC CTTGCAGACC 
ATTCCCCAGG GCACGTTCAT CTGCGAGTAC GTCGGGGAGC TGATCTCCGA CGCCGAGGCA 
GACGTGAGAG AGGATGATTC TTACCTCTTC GACTTAGACA ACAAGGATGG TGAGGTCTAC 
TGCATTGATG CCCGTTACTA TGGCAACATC AGCCGCTTCA TTAACCACCT GTGCGACCCC 
AACATCATCC CTGTTCGGGT CTTCATGCTG CATCAAGACC TACGGTTCCC TCGCATCGCC 
TTCTTCAGCT CCAGGGACAT CCGGACCGGG GAGGAGCTGG GGTTTGACTA CGGCGACCGG 
TTCTGGGATA TCAAGAGCAA GTATTTCACC TGCCAGTGTG GCTCTGAGAA GTGCAAGCAT 
TCAGCTGAGG CCATCGCCCT GGAGCAGAGC CGCCTGGCCC GTCTGGACCC CCACCCGGAG 
CTGCTCCCTG ACCTCAGCTC CCTGCCCCCC ATCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256016.2
CDS	16..3837
Translation	MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPSQGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESESLPSPPPSPGRRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255954.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255954.3

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGAGTGAA TGGTGTGGGT TCCTCAGGGC CCAGTGAGTA CATGGAGGTT 
CCTCTGGGGT CCCTGGAGCT GCCCAGCGAG GGGACCCTCT CCCCCAACCA CGCTGGGGTC 
TCCAATGACA CGTCTTCGCT GGAGACGGAG CGTGGGTTTG AGGAGCTGCC CCTCTGCAGC 
TGCCGCATGG AGGCGCCCAA GATTGACCGC ATCAGCGAGA GAGCAGGACA CAAGTGTATG 
GCCACCGAAA GTGTGGACGG AGAGCTGCTG GGTTGCAATG CCGCCATCTT GAAGCGGGAG 
ACCATGCGGC CGTCTAGCCG TGTGGCGCTG ATGGTACTGT GTGAGGCCCA TCGAGCCCGC 
ATGGTCAAGC ACCATTGCTG CCCAGGCTGT GGCTACTTCT GCACGGCGGG CACCTTCCTG 
GAGTGTCACC CCGACTTCCG TGTAGCTCAC CGCTTCCATA AGGCCTGCGT GTCCCAGCTC 
AATGGGATGG TTTTCTGTCC CCACTGTGGG GAGGACGCCT CAGAGGCCCA GGAGGTGACC 
ATCCCTCGGG GCGATGGGGG AACACCCCCG GTTGGCACTG TGGCGCCTGC CCCGCCACCC 
CTGGCACACG ACGCCCCAGG GCGAGCGGAT ACCTCCCAGC CCAGTGCCCG AATGCGAGGA 
CACGGAGAGC CAAGGCGCCC ACCCTGTGAT CCCCTGGCCG ACACCATTGA CAGCTCGGGG 
CCTTCTCTGA CTCTGCCTAA TGGCGGCTGC CTCTCAGCTG TGGGTCTGCC CCCAGGGCCA 
GGCCGGGAAG CCCTGGAAAA AGCCTTGGTC ATCCAGGAGT CCGAGAGCCT ACCTTCTCCC 
CCTCCGTCCC CGGGCAGGAG GAAGAAGCTG CGGTTCCACC CGCGGCAGCT CTACCTGTCG 
GTGAAGCAGG GGGAGCTGCA GAAGGTGATC CTCATGCTGC TCGACAACCT GGACCCCAAC 
TTCCAGAGCG ACCAGCAGAG CAAGCGCACG CCCCTGCACG CAGCCGCCCA GAAGGGGTCC 
GTGGAGATCT GTCACGTGCT GCTACAGGCA GGAGCCAACA TCAACGCCGT GGATAAGCAG 
CAGCGCACCC CGCTGATGGA GGCCGTGGTG AACAACCACC TGGAGGTGGC TCGCTACATG 
GTGCAGCTGG GCGGCTGTGT GTACAGCAAG GAAGAGGATG GCTCTACCTG TCTCCATCAT 
GCGGCCAAAA TTGGGAACTT GGAAATGGTC AGCCTGCTGC TGAGCACAGG GCAGGTGGAC 
GTCAATGCCC AGGACAGTGG GGGCTGGACA CCCATCATCT GGGCAGCCGA GCACAAGCAC 
ATCGACGTGA TCCGTATGCT GCTGACCCGG GGCGCCGACG TCACCCTTAC CGACAACGAG 
GAAAACATCT GCCTGCACTG GGCTTCCTTC ACGGGTAGTG CCGCTATTGC CGAGGTCCTC 
CTGAATGCCC AGTGCGACCT CCATGCTGTC AACTACCACG GGGACACGCC CCTGCACATA 
GCCGCCAGGG AGAGCTACCA TGACTGCGTT CTGTTGTTCC TGTCCCGTGG AGCCAACCCT 
GAGCTTCGGA ACAAAGAAGG GGACACGGCG TGGGATCTGA CCCCAGAGCG CTCCGATGTG 
TGGTTTGCAC TGCAGCTCAA TCGCAAGCTT AGGCTCGGGG TAGGGAACCG GGCTGTCCGC 
ACCGAGAAGA TCATCTGCCG GGATGTAGCC CGAGGCTATG AGAATGTGCC CATTCCCTGT 
GTCAATGGCG TGGATGGGGA GCCATGCCCA GAGGACTATA AGTACATCTC CGAGAACTGT 
GAAACGTCCA CCATGAACAT CGACCGAAAC ATCACTCACC TGCAGCACTG CACGTGTGTT 
GACGACTGCT CCAGCTCCAA CTGCCTGTGT GGCCAGCTAA GCATCCGATG CTGGTATGAT 
AAGGACGGGC GGCTGCTCCA GGAGTTTAAC AAGATCGAGC CCCCCCTGAT CTTTGAGTGT 
AACCAGGCAT GCTCCTGCTG GAGAAGCTGC AAGAACCGCG TGGTGCAGAG CGGCATCAAG 
GTGCGGCTGC AGCTCTACCG GACGGCCAAG ATGGGCTGGG GGGTCCGTGC CTTGCAGACC 
ATTCCCCAGG GCACGTTCAT CTGCGAGTAC GTCGGGGAGC TGATCTCCGA CGCCGAGGCA 
GACGTGAGAG AGGATGATTC TTACCTCTTC GACTTAGACA ACAAGGATGG TGAGGTCTAC 
TGCATTGATG CCCGTTACTA TGGCAACATC AGCCGCTTCA TTAACCACCT GTGCGACCCC 
AACATCATCC CTGTTCGGGT CTTCATGCTG CATCAAGACC TACGGTTCCC TCGCATCGCC 
TTCTTCAGCT CCAGGGACAT CCGGACCGGG GAGGAGCTGG GGTTTGACTA CGGCGACCGG 
TTCTGGGATA TCAAGAGCAA GTATTTCACC TGCCAGTGTG GCTCTGAGAA GTGCAAGCAT 
TCAGCTGAGG CCATCGCCCT GGAGCAGAGC CGCCTGGCCC GTCTGGACCC CCACCCGGAG 
CTGCTCCCTG ACCTCAGCTC CCTGCCCCCC ATCAACACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255955.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256017.2 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X2, mRNA.

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CloneID	ORa48021
Clone ID Related Accession (Same CDS sequence)	XM_006255955.3
Accession Version	XM_006255955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGGGTCTC CAATGACACG TCTTCGCTGG AGACGGAGCG TGGGTTTGAG 
GAGCTGCCCC TCTGCAGCTG CCGCATGGAG GCGCCCAAGA TTGACCGCAT CAGCGAGAGA 
GCAGGACACA AGTGTATGGC CACCGAAAGT GTGGACGGAG AGCTGCTGGG TTGCAATGCC 
GCCATCTTGA AGCGGGAGAC CATGCGGCCG TCTAGCCGTG TGGCGCTGAT GGTACTGTGT 
GAGGCCCATC GAGCCCGCAT GGTCAAGCAC CATTGCTGCC CAGGCTGTGG CTACTTCTGC 
ACGGCGGGCA CCTTCCTGGA GTGTCACCCC GACTTCCGTG TAGCTCACCG CTTCCATAAG 
GCCTGCGTGT CCCAGCTCAA TGGGATGGTT TTCTGTCCCC ACTGTGGGGA GGACGCCTCA 
GAGGCCCAGG AGGTGACCAT CCCTCGGGGC GATGGGGGAA CACCCCCGGT TGGCACTGTG 
GCGCCTGCCC CGCCACCCCT GGCACACGAC GCCCCAGGGC GAGCGGATAC CTCCCAGCCC 
AGTGCCCGAA TGCGAGGACA CGGAGAGCCA AGGCGCCCAC CCTGTGATCC CCTGGCCGAC 
ACCATTGACA GCTCGGGGCC TTCTCTGACT CTGCCTAATG GCGGCTGCCT CTCAGCTGTG 
GGTCTGCCCC CAGGGCCAGG CCGGGAAGCC CTGGAAAAAG CCTTGGTCAT CCAGGAGTCC 
GAGAGCCTAC CTTCTCCCCC TCCGTCCCCG GGCAGGAGGA AGAAGCTGCG GTTCCACCCG 
CGGCAGCTCT ACCTGTCGGT GAAGCAGGGG GAGCTGCAGA AGGTGATCCT CATGCTGCTC 
GACAACCTGG ACCCCAACTT CCAGAGCGAC CAGCAGAGCA AGCGCACGCC CCTGCACGCA 
GCCGCCCAGA AGGGGTCCGT GGAGATCTGT CACGTGCTGC TACAGGCAGG AGCCAACATC 
AACGCCGTGG ATAAGCAGCA GCGCACCCCG CTGATGGAGG CCGTGGTGAA CAACCACCTG 
GAGGTGGCTC GCTACATGGT GCAGCTGGGC GGCTGTGTGT ACAGCAAGGA AGAGGATGGC 
TCTACCTGTC TCCATCATGC GGCCAAAATT GGGAACTTGG AAATGGTCAG CCTGCTGCTG 
AGCACAGGGC AGGTGGACGT CAATGCCCAG GACAGTGGGG GCTGGACACC CATCATCTGG 
GCAGCCGAGC ACAAGCACAT CGACGTGATC CGTATGCTGC TGACCCGGGG CGCCGACGTC 
ACCCTTACCG ACAACGAGGA AAACATCTGC CTGCACTGGG CTTCCTTCAC GGGTAGTGCC 
GCTATTGCCG AGGTCCTCCT GAATGCCCAG TGCGACCTCC ATGCTGTCAA CTACCACGGG 
GACACGCCCC TGCACATAGC CGCCAGGGAG AGCTACCATG ACTGCGTTCT GTTGTTCCTG 
TCCCGTGGAG CCAACCCTGA GCTTCGGAAC AAAGAAGGGG ACACGGCGTG GGATCTGACC 
CCAGAGCGCT CCGATGTGTG GTTTGCACTG CAGCTCAATC GCAAGCTTAG GCTCGGGGTA 
GGGAACCGGG CTGTCCGCAC CGAGAAGATC ATCTGCCGGG ATGTAGCCCG AGGCTATGAG 
AATGTGCCCA TTCCCTGTGT CAATGGCGTG GATGGGGAGC CATGCCCAGA GGACTATAAG 
TACATCTCCG AGAACTGTGA AACGTCCACC ATGAACATCG ACCGAAACAT CACTCACCTG 
CAGCACTGCA CGTGTGTTGA CGACTGCTCC AGCTCCAACT GCCTGTGTGG CCAGCTAAGC 
ATCCGATGCT GGTATGATAA GGACGGGCGG CTGCTCCAGG AGTTTAACAA GATCGAGCCC 
CCCCTGATCT TTGAGTGTAA CCAGGCATGC TCCTGCTGGA GAAGCTGCAA GAACCGCGTG 
GTGCAGAGCG GCATCAAGGT GCGGCTGCAG CTCTACCGGA CGGCCAAGAT GGGCTGGGGG 
GTCCGTGCCT TGCAGACCAT TCCCCAGGGC ACGTTCATCT GCGAGTACGT CGGGGAGCTG 
ATCTCCGACG CCGAGGCAGA CGTGAGAGAG GATGATTCTT ACCTCTTCGA CTTAGACAAC 
AAGGATGGTG AGGTCTACTG CATTGATGCC CGTTACTATG GCAACATCAG CCGCTTCATT 
AACCACCTGT GCGACCCCAA CATCATCCCT GTTCGGGTCT TCATGCTGCA TCAAGACCTA 
CGGTTCCCTC GCATCGCCTT CTTCAGCTCC AGGGACATCC GGACCGGGGA GGAGCTGGGG 
TTTGACTACG GCGACCGGTT CTGGGATATC AAGAGCAAGT ATTTCACCTG CCAGTGTGGC 
TCTGAGAAGT GCAAGCATTC AGCTGAGGCC ATCGCCCTGG AGCAGAGCCG CCTGGCCCGT 
CTGGACCCCC ACCCGGAGCT GCTCCCTGAC CTCAGCTCCC TGCCCCCCAT CAACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256017.2
CDS	16..3735
Translation	MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPSQGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESESLPSPPPSPGRRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255955.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255955.3

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGGGTCTC CAATGACACG TCTTCGCTGG AGACGGAGCG TGGGTTTGAG 
GAGCTGCCCC TCTGCAGCTG CCGCATGGAG GCGCCCAAGA TTGACCGCAT CAGCGAGAGA 
GCAGGACACA AGTGTATGGC CACCGAAAGT GTGGACGGAG AGCTGCTGGG TTGCAATGCC 
GCCATCTTGA AGCGGGAGAC CATGCGGCCG TCTAGCCGTG TGGCGCTGAT GGTACTGTGT 
GAGGCCCATC GAGCCCGCAT GGTCAAGCAC CATTGCTGCC CAGGCTGTGG CTACTTCTGC 
ACGGCGGGCA CCTTCCTGGA GTGTCACCCC GACTTCCGTG TAGCTCACCG CTTCCATAAG 
GCCTGCGTGT CCCAGCTCAA TGGGATGGTT TTCTGTCCCC ACTGTGGGGA GGACGCCTCA 
GAGGCCCAGG AGGTGACCAT CCCTCGGGGC GATGGGGGAA CACCCCCGGT TGGCACTGTG 
GCGCCTGCCC CGCCACCCCT GGCACACGAC GCCCCAGGGC GAGCGGATAC CTCCCAGCCC 
AGTGCCCGAA TGCGAGGACA CGGAGAGCCA AGGCGCCCAC CCTGTGATCC CCTGGCCGAC 
ACCATTGACA GCTCGGGGCC TTCTCTGACT CTGCCTAATG GCGGCTGCCT CTCAGCTGTG 
GGTCTGCCCC CAGGGCCAGG CCGGGAAGCC CTGGAAAAAG CCTTGGTCAT CCAGGAGTCC 
GAGAGCCTAC CTTCTCCCCC TCCGTCCCCG GGCAGGAGGA AGAAGCTGCG GTTCCACCCG 
CGGCAGCTCT ACCTGTCGGT GAAGCAGGGG GAGCTGCAGA AGGTGATCCT CATGCTGCTC 
GACAACCTGG ACCCCAACTT CCAGAGCGAC CAGCAGAGCA AGCGCACGCC CCTGCACGCA 
GCCGCCCAGA AGGGGTCCGT GGAGATCTGT CACGTGCTGC TACAGGCAGG AGCCAACATC 
AACGCCGTGG ATAAGCAGCA GCGCACCCCG CTGATGGAGG CCGTGGTGAA CAACCACCTG 
GAGGTGGCTC GCTACATGGT GCAGCTGGGC GGCTGTGTGT ACAGCAAGGA AGAGGATGGC 
TCTACCTGTC TCCATCATGC GGCCAAAATT GGGAACTTGG AAATGGTCAG CCTGCTGCTG 
AGCACAGGGC AGGTGGACGT CAATGCCCAG GACAGTGGGG GCTGGACACC CATCATCTGG 
GCAGCCGAGC ACAAGCACAT CGACGTGATC CGTATGCTGC TGACCCGGGG CGCCGACGTC 
ACCCTTACCG ACAACGAGGA AAACATCTGC CTGCACTGGG CTTCCTTCAC GGGTAGTGCC 
GCTATTGCCG AGGTCCTCCT GAATGCCCAG TGCGACCTCC ATGCTGTCAA CTACCACGGG 
GACACGCCCC TGCACATAGC CGCCAGGGAG AGCTACCATG ACTGCGTTCT GTTGTTCCTG 
TCCCGTGGAG CCAACCCTGA GCTTCGGAAC AAAGAAGGGG ACACGGCGTG GGATCTGACC 
CCAGAGCGCT CCGATGTGTG GTTTGCACTG CAGCTCAATC GCAAGCTTAG GCTCGGGGTA 
GGGAACCGGG CTGTCCGCAC CGAGAAGATC ATCTGCCGGG ATGTAGCCCG AGGCTATGAG 
AATGTGCCCA TTCCCTGTGT CAATGGCGTG GATGGGGAGC CATGCCCAGA GGACTATAAG 
TACATCTCCG AGAACTGTGA AACGTCCACC ATGAACATCG ACCGAAACAT CACTCACCTG 
CAGCACTGCA CGTGTGTTGA CGACTGCTCC AGCTCCAACT GCCTGTGTGG CCAGCTAAGC 
ATCCGATGCT GGTATGATAA GGACGGGCGG CTGCTCCAGG AGTTTAACAA GATCGAGCCC 
CCCCTGATCT TTGAGTGTAA CCAGGCATGC TCCTGCTGGA GAAGCTGCAA GAACCGCGTG 
GTGCAGAGCG GCATCAAGGT GCGGCTGCAG CTCTACCGGA CGGCCAAGAT GGGCTGGGGG 
GTCCGTGCCT TGCAGACCAT TCCCCAGGGC ACGTTCATCT GCGAGTACGT CGGGGAGCTG 
ATCTCCGACG CCGAGGCAGA CGTGAGAGAG GATGATTCTT ACCTCTTCGA CTTAGACAAC 
AAGGATGGTG AGGTCTACTG CATTGATGCC CGTTACTATG GCAACATCAG CCGCTTCATT 
AACCACCTGT GCGACCCCAA CATCATCCCT GTTCGGGTCT TCATGCTGCA TCAAGACCTA 
CGGTTCCCTC GCATCGCCTT CTTCAGCTCC AGGGACATCC GGACCGGGGA GGAGCTGGGG 
TTTGACTACG GCGACCGGTT CTGGGATATC AAGAGCAAGT ATTTCACCTG CCAGTGTGGC 
TCTGAGAAGT GCAAGCATTC AGCTGAGGCC ATCGCCCTGG AGCAGAGCCG CCTGGCCCGT 
CTGGACCCCC ACCCGGAGCT GCTCCCTGAC CTCAGCTCCC TGCCCCCCAT CAACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ehmt2 ( XM_006255956.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256018.2 Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X3, mRNA.

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CloneID	ORa48022
Clone ID Related Accession (Same CDS sequence)	XM_006255956.3
Accession Version	XM_006255956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone-lysine N-methyltransferase EHMT2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGGGTCTC CAATGACACG TCTTCGCTGG AGACGGAGCG TGGGTTTGAG 
GAGCTGCCCC TCTGCAGCTG CCGCATGGAG GCGCCCAAGA TTGACCGCAT CAGCGAGAGA 
GCAGGACACA AGTGTATGGC CACCGAAAGT GTGGACGGAG AGCTGCTGGG TTGCAATGCC 
GCCATCTTGA AGCGGGAGAC CATGCGGCCG TCTAGCCGTG TGGCGCTGAT GGTACTGTGT 
GAGGCCCATC GAGCCCGCAT GGTCAAGCAC CATTGCTGCC CAGGCTGTGG CTACTTCTGC 
ACGGCGGGCA CCTTCCTGGA GTGTCACCCC GACTTCCGTG TAGCTCACCG CTTCCATAAG 
GCCTGCGTGT CCCAGCTCAA TGGGATGGTT TTCTGTCCCC ACTGTGGGGA GGACGCCTCA 
GAGGCCCAGG AGGTGACCAT CCCTCGGGGC GATGGGGGAA CACCCCCGGT TGGCACTGTG 
GCGCCTGCCC CGCCACCCCT GGCACACGAC GCCCCAGGGC GAGCGGATAC CTCCCAGCCC 
AGTGCCCGAA TGCGAGGACA CGGAGAGCCA AGGCGCCCAC CCTGTGATCC CCTGGCCGAC 
ACCATTGACA GCTCGGGGCC TTCTCTGACT CTGCCTAATG GCGGCTGCCT CTCAGCTGTG 
GGTCTGCCCC CAGGGCCAGG CCGGGAAGCC CTGGAAAAAG CCTTGGTCAT CCAGGAGTCC 
GAGAGGAGGA AGAAGCTGCG GTTCCACCCG CGGCAGCTCT ACCTGTCGGT GAAGCAGGGG 
GAGCTGCAGA AGGTGATCCT CATGCTGCTC GACAACCTGG ACCCCAACTT CCAGAGCGAC 
CAGCAGAGCA AGCGCACGCC CCTGCACGCA GCCGCCCAGA AGGGGTCCGT GGAGATCTGT 
CACGTGCTGC TACAGGCAGG AGCCAACATC AACGCCGTGG ATAAGCAGCA GCGCACCCCG 
CTGATGGAGG CCGTGGTGAA CAACCACCTG GAGGTGGCTC GCTACATGGT GCAGCTGGGC 
GGCTGTGTGT ACAGCAAGGA AGAGGATGGC TCTACCTGTC TCCATCATGC GGCCAAAATT 
GGGAACTTGG AAATGGTCAG CCTGCTGCTG AGCACAGGGC AGGTGGACGT CAATGCCCAG 
GACAGTGGGG GCTGGACACC CATCATCTGG GCAGCCGAGC ACAAGCACAT CGACGTGATC 
CGTATGCTGC TGACCCGGGG CGCCGACGTC ACCCTTACCG ACAACGAGGA AAACATCTGC 
CTGCACTGGG CTTCCTTCAC GGGTAGTGCC GCTATTGCCG AGGTCCTCCT GAATGCCCAG 
TGCGACCTCC ATGCTGTCAA CTACCACGGG GACACGCCCC TGCACATAGC CGCCAGGGAG 
AGCTACCATG ACTGCGTTCT GTTGTTCCTG TCCCGTGGAG CCAACCCTGA GCTTCGGAAC 
AAAGAAGGGG ACACGGCGTG GGATCTGACC CCAGAGCGCT CCGATGTGTG GTTTGCACTG 
CAGCTCAATC GCAAGCTTAG GCTCGGGGTA GGGAACCGGG CTGTCCGCAC CGAGAAGATC 
ATCTGCCGGG ATGTAGCCCG AGGCTATGAG AATGTGCCCA TTCCCTGTGT CAATGGCGTG 
GATGGGGAGC CATGCCCAGA GGACTATAAG TACATCTCCG AGAACTGTGA AACGTCCACC 
ATGAACATCG ACCGAAACAT CACTCACCTG CAGCACTGCA CGTGTGTTGA CGACTGCTCC 
AGCTCCAACT GCCTGTGTGG CCAGCTAAGC ATCCGATGCT GGTATGATAA GGACGGGCGG 
CTGCTCCAGG AGTTTAACAA GATCGAGCCC CCCCTGATCT TTGAGTGTAA CCAGGCATGC 
TCCTGCTGGA GAAGCTGCAA GAACCGCGTG GTGCAGAGCG GCATCAAGGT GCGGCTGCAG 
CTCTACCGGA CGGCCAAGAT GGGCTGGGGG GTCCGTGCCT TGCAGACCAT TCCCCAGGGC 
ACGTTCATCT GCGAGTACGT CGGGGAGCTG ATCTCCGACG CCGAGGCAGA CGTGAGAGAG 
GATGATTCTT ACCTCTTCGA CTTAGACAAC AAGGATGGTG AGGTCTACTG CATTGATGCC 
CGTTACTATG GCAACATCAG CCGCTTCATT AACCACCTGT GCGACCCCAA CATCATCCCT 
GTTCGGGTCT TCATGCTGCA TCAAGACCTA CGGTTCCCTC GCATCGCCTT CTTCAGCTCC 
AGGGACATCC GGACCGGGGA GGAGCTGGGG TTTGACTACG GCGACCGGTT CTGGGATATC 
AAGAGCAAGT ATTTCACCTG CCAGTGTGGC TCTGAGAAGT GCAAGCATTC AGCTGAGGCC 
ATCGCCCTGG AGCAGAGCCG CCTGGCCCGT CTGGACCCCC ACCCGGAGCT GCTCCCTGAC 
CTCAGCTCCC TGCCCCCCAT CAACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256018.2
CDS	16..3705
Translation	MRGLPRGRGLMRARGRGRAAPTGGRGRGRGGAHRGRGRPRSLLSLPRAQASWAPQLPAGLTGPPVPCLPSQGEAPAEMGALLLEKEPRGAAERVHGSLGDTSHSEETLPKANPDSLEPTGPSSPASVTVTVGDEGADTPVGATPLIGEEPENLEGDGGRILLGHATKSFPSSPSKGGACPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAATAGPEPPPATTAGQEGQPKVHRARKTMSKPSNGQPPVPEKRPPEVQHFRMSDDMHLGKVTSDVAKRRKLTSGSLSEDLGSAGGSGEVILEKGEPRPLEEWETVVGDDFSLYYDAYSVDERVDSDSKSEVEALAEQLSEEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELLGCNAAILKRETMRPSSRVALMVLCEAHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGGTPPVGTVAPAPPPLAHDAPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPPGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQLGGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKHIDVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNAQCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAVRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRSCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPDLSSLPPINT

Target ORF information:

RefSeq Version	XM_006255956.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus euchromatic histone lysine methyltransferase 2 (Ehmt2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255956.3

ORF Insert Sequence:

ATGCGGGGTC TGCCGAGAGG GAGGGGGCTG ATGCGGGCCC GGGGGCGGGG GCGTGCGGCC 
CCCACGGGCG GCCGCGGCCG GGGTCGCGGG GGCGCCCACC GTGGACGAGG TAGGCCCCGA 
AGCCTGCTCT CGCTGCCCAG GGCCCAGGCG TCTTGGGCCC CCCAGCTGCC TGCCGGGCTG 
ACCGGCCCTC CGGTCCCTTG TCTCCCCTCC CAGGGGGAGG CCCCCGCTGA GATGGGGGCG 
CTGCTGCTGG AGAAGGAGCC CCGAGGAGCC GCCGAGAGAG TTCATGGCTC TTTGGGGGAC 
ACCTCTCATA GTGAGGAGAC CCTTCCCAAG GCCAACCCCG ACTCCTTGGA GCCTACCGGC 
CCCTCCTCTC CGGCCTCTGT CACTGTCACC GTCGGCGATG AGGGGGCTGA CACCCCTGTC 
GGGGCCACAC CGCTCATCGG GGAGGAACCC GAGAACCTGG AGGGAGATGG GGGTCGAATC 
CTGCTGGGCC ATGCCACAAA GTCATTCCCC TCTTCCCCCA GCAAGGGGGG TGCCTGTCCC 
AGTCGGGCCA AAATGTCAAT GACAGGGGCA GGAAAGTCGC CCCCCTCGGT CCAGAGTTTG 
GCCATGAGGC TGTTGAGCAT GCCTGGGGCC CAGGGAGCTG CAACTGCTGG GCCTGAGCCC 
CCTCCGGCAA CAACTGCCGG CCAGGAGGGG CAGCCCAAAG TACACCGAGC TCGGAAAACC 
ATGTCCAAGC CTAGCAACGG ACAGCCTCCA GTCCCTGAGA AGCGGCCCCC TGAAGTCCAG 
CATTTCCGCA TGAGTGACGA CATGCATCTG GGAAAGGTGA CTTCAGATGT GGCCAAACGG 
AGGAAGTTGA CCTCGGGCAG CCTGTCAGAG GACTTGGGCT CTGCTGGGGG CTCAGGAGAA 
GTGATCCTGG AGAAGGGAGA GCCCAGGCCT CTGGAGGAGT GGGAGACGGT GGTGGGCGAT 
GACTTCAGCC TGTACTATGA CGCGTACTCT GTGGATGAGC GGGTGGACTC TGACAGCAAG 
TCTGAAGTCG AAGCTCTAGC TGAACAGTTG AGTGAAGAGG AGGAGGAGGA AGAGGAGGAA 
GAAGAAGAGG AAGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGGAGGAGGA TGAGGAGTCG 
GGCAACCAGT CAGACAGGAG TGGCTCTAGC GGCCGACGCA AGGCCAAGAA GAAATGGCGG 
AAAGACAGCC CATGGGTGAA GCCATCCAGG AAACGGCGGA AGCGAGAGCC TCCGAGGGCC 
AAGGAGCCCA GAGGGGTCTC CAATGACACG TCTTCGCTGG AGACGGAGCG TGGGTTTGAG 
GAGCTGCCCC TCTGCAGCTG CCGCATGGAG GCGCCCAAGA TTGACCGCAT CAGCGAGAGA 
GCAGGACACA AGTGTATGGC CACCGAAAGT GTGGACGGAG AGCTGCTGGG TTGCAATGCC 
GCCATCTTGA AGCGGGAGAC CATGCGGCCG TCTAGCCGTG TGGCGCTGAT GGTACTGTGT 
GAGGCCCATC GAGCCCGCAT GGTCAAGCAC CATTGCTGCC CAGGCTGTGG CTACTTCTGC 
ACGGCGGGCA CCTTCCTGGA GTGTCACCCC GACTTCCGTG TAGCTCACCG CTTCCATAAG 
GCCTGCGTGT CCCAGCTCAA TGGGATGGTT TTCTGTCCCC ACTGTGGGGA GGACGCCTCA 
GAGGCCCAGG AGGTGACCAT CCCTCGGGGC GATGGGGGAA CACCCCCGGT TGGCACTGTG 
GCGCCTGCCC CGCCACCCCT GGCACACGAC GCCCCAGGGC GAGCGGATAC CTCCCAGCCC 
AGTGCCCGAA TGCGAGGACA CGGAGAGCCA AGGCGCCCAC CCTGTGATCC CCTGGCCGAC 
ACCATTGACA GCTCGGGGCC TTCTCTGACT CTGCCTAATG GCGGCTGCCT CTCAGCTGTG 
GGTCTGCCCC CAGGGCCAGG CCGGGAAGCC CTGGAAAAAG CCTTGGTCAT CCAGGAGTCC 
GAGAGGAGGA AGAAGCTGCG GTTCCACCCG CGGCAGCTCT ACCTGTCGGT GAAGCAGGGG 
GAGCTGCAGA AGGTGATCCT CATGCTGCTC GACAACCTGG ACCCCAACTT CCAGAGCGAC 
CAGCAGAGCA AGCGCACGCC CCTGCACGCA GCCGCCCAGA AGGGGTCCGT GGAGATCTGT 
CACGTGCTGC TACAGGCAGG AGCCAACATC AACGCCGTGG ATAAGCAGCA GCGCACCCCG 
CTGATGGAGG CCGTGGTGAA CAACCACCTG GAGGTGGCTC GCTACATGGT GCAGCTGGGC 
GGCTGTGTGT ACAGCAAGGA AGAGGATGGC TCTACCTGTC TCCATCATGC GGCCAAAATT 
GGGAACTTGG AAATGGTCAG CCTGCTGCTG AGCACAGGGC AGGTGGACGT CAATGCCCAG 
GACAGTGGGG GCTGGACACC CATCATCTGG GCAGCCGAGC ACAAGCACAT CGACGTGATC 
CGTATGCTGC TGACCCGGGG CGCCGACGTC ACCCTTACCG ACAACGAGGA AAACATCTGC 
CTGCACTGGG CTTCCTTCAC GGGTAGTGCC GCTATTGCCG AGGTCCTCCT GAATGCCCAG 
TGCGACCTCC ATGCTGTCAA CTACCACGGG GACACGCCCC TGCACATAGC CGCCAGGGAG 
AGCTACCATG ACTGCGTTCT GTTGTTCCTG TCCCGTGGAG CCAACCCTGA GCTTCGGAAC 
AAAGAAGGGG ACACGGCGTG GGATCTGACC CCAGAGCGCT CCGATGTGTG GTTTGCACTG 
CAGCTCAATC GCAAGCTTAG GCTCGGGGTA GGGAACCGGG CTGTCCGCAC CGAGAAGATC 
ATCTGCCGGG ATGTAGCCCG AGGCTATGAG AATGTGCCCA TTCCCTGTGT CAATGGCGTG 
GATGGGGAGC CATGCCCAGA GGACTATAAG TACATCTCCG AGAACTGTGA AACGTCCACC 
ATGAACATCG ACCGAAACAT CACTCACCTG CAGCACTGCA CGTGTGTTGA CGACTGCTCC 
AGCTCCAACT GCCTGTGTGG CCAGCTAAGC ATCCGATGCT GGTATGATAA GGACGGGCGG 
CTGCTCCAGG AGTTTAACAA GATCGAGCCC CCCCTGATCT TTGAGTGTAA CCAGGCATGC 
TCCTGCTGGA GAAGCTGCAA GAACCGCGTG GTGCAGAGCG GCATCAAGGT GCGGCTGCAG 
CTCTACCGGA CGGCCAAGAT GGGCTGGGGG GTCCGTGCCT TGCAGACCAT TCCCCAGGGC 
ACGTTCATCT GCGAGTACGT CGGGGAGCTG ATCTCCGACG CCGAGGCAGA CGTGAGAGAG 
GATGATTCTT ACCTCTTCGA CTTAGACAAC AAGGATGGTG AGGTCTACTG CATTGATGCC 
CGTTACTATG GCAACATCAG CCGCTTCATT AACCACCTGT GCGACCCCAA CATCATCCCT 
GTTCGGGTCT TCATGCTGCA TCAAGACCTA CGGTTCCCTC GCATCGCCTT CTTCAGCTCC 
AGGGACATCC GGACCGGGGA GGAGCTGGGG TTTGACTACG GCGACCGGTT CTGGGATATC 
AAGAGCAAGT ATTTCACCTG CCAGTGTGGC TCTGAGAAGT GCAAGCATTC AGCTGAGGCC 
ATCGCCCTGG AGCAGAGCCG CCTGGCCCGT CTGGACCCCC ACCCGGAGCT GCTCCCTGAC 
CTCAGCTCCC TGCCCCCCAT CAACACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of the histone dimethyltransferase G9a in nucleus accumbens shell increases cocaine self-administration, stress-induced reinstatement, and anxiety.
The Journal of neuroscience : the official journal of the Society for Neuroscience(2017 Dec)
Anderson EM,Larson EB,Guzman D,Marie Wissman A,Neve RL,Nestler EJ,Self DW

Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons.
Aging cell16(5)1062-1072(2017 Oct)
Sharma M,Dierkes T,Sajikumar S

The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy.
The Journal of clinical investigation127(1)335-348(2017 Jan)
Thienpont B,Aronsen JM,Robinson EL,Okkenhaug H,Loche E,Ferrini A,Brien P,Alkass K,Tomasso A,Agrawal A,Bergmann O,Sjaastad I,Reik W,Roderick HL

Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-mediated Transcriptional Repression of ?-Opioid Receptors in Primary Sensory Neurons.
The Journal of biological chemistry291(16)8475-85(2016 Apr)
Zhang Y,Chen SR,Laumet G,Chen H,Pan HL

H3K9 histone methyltransferase G9a ameliorates dilated cardiomyopathy via the downregulation of cell adhesion molecules.
Molecular medicine reports11(5)3872-9(2015 May)
Chen G,Wang X,Zhang Y,Ru X,Zhou L,Tian Y

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Ehmt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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