Tkfc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tkfc
Entrez Gene ID	361730
Full Name	triokinase and FMN cyclase
Synonyms	Dak,RGD1311026
General protein information	Preferred Names triokinase and FMN cyclase Names triokinase/FMN cyclase bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) dihydroxyacetone kinase 2 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231057.2	XP_006231119.2	triokinase/FMN cyclase isoform X1
XM_006231056.3	XP_006231118.1	triokinase/FMN cyclase isoform X2
NM_001039031.1	NP_001034120.1	triokinase/FMN cyclase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DAK	NP_056348.2
Caenorhabditis elegans (roundworm)	W02H5.8	NP_503724.1
Canis lupus familiaris (dog)	DAK	XP_005631736.1
Bos taurus (cattle)	DAK	NP_001019695.2
Magnaporthe oryzae (rice blast fungus)	MGG_03095	XP_003720578.1
Arabidopsis thaliana (thale cress)	AT3G17770	NP_188404.1
Arabidopsis thaliana (thale cress)	AT1G48430	NP_175276.3
Gallus gallus (chicken)	DAK	XP_424258.4
Danio rerio (zebrafish)	dak	NP_001070098.1
Pan troglodytes (chimpanzee)	DAK	NP_001233523.1
Mus musculus (house mouse)	Dak	NP_663471.1
Rattus norvegicus (Norway rat)	Dak	NP_001034120.1
Eremothecium gossypii	AGOS_AER139C	NP_984998.2
Neurospora crassa	NCU04400	XP_957477.1
Oryza sativa (rice)	Os03g0719300	NP_001051093.1
Xenopus tropicalis (tropical clawed frog)	dak	XP_002937699.2

Gene Ontology
Bibliography

Related articles in PubMed

Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements.
Rodrigues JR, Couto A, Cabezas A, Pinto RM, Ribeiro JM, Canales J, Costas MJ, Cameselle JC
The Journal of biological chemistry289(15)10620-36(2014 Apr)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase.
Diao F, Li S, Tian Y, Zhang M, Xu LG, Zhang Y, Wang RP, Chen D, Zhai Z, Zhong B, Tien P, Shu HB
Proceedings of the National Academy of Sciences of the United States of America104(28)11706-11(2007 Jul)

Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases.
Cabezas A, Costas MJ, Pinto RM, Couto A, Cameselle JC
Biochemical and biophysical research communications338(4)1682-9(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tkfc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tkfc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08254	NM_001039031.1 Latest version!	Rattus norvegicus triokinase and FMN cyclase (Tkfc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa08254	XM_006231056.3 Latest version!	Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa47998	XM_006231057.2 Latest version!	Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tkfc ( NM_001039031.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001034120.1 Rattus norvegicus triokinase and FMN cyclase (Tkfc), mRNA.

Price & Availability↑

CloneID	ORa08254
Clone ID Related Accession (Same CDS sequence)	XM_006231056.3 , NM_001039031.1
Accession Version	NM_001039031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	triokinase/FMN cyclase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098925.1. ##Evidence-Data-START## Transcript exon combination :: BC098925.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTCCA AGAAGATGGT GAATTCTGTG GAAGGGTGTG CTGGTGATGC CCTTGCTGGG 
TTTGTTGCCT GTAACCCTGA CTTGCAGCTC CTGCAAGGGT ACCGTGTAGC CCTACGTTCT 
GACCTGGACA GCCTCAAGGG CCGGGTGGCA CTTCTGTCAG GTGGAGGCTC AGGCCATGAG 
CCTGCCCATG CCGGTTTTAT CGGGAAAGGA ATGCTGACAG GTGTCATTGC AGGAGCAGTG 
TTTGCCTCTC CTGCTGTGGG CAGCATCCTG GCAGCCATTA GAGCTGTGGC CCAGGCAGGC 
ACAGCCGGCA CCCTCCTCAT CGTGAAGAAC TACACTGGGG ATCGGCTCAA CTTTGGACTT 
GCCATGGAGC AGGCCAAGGC CGAGGGCATC TCTGTGGAGA TGGTGGTAAT TGAGGATGAC 
AGCGCCTTCA CTGTCCTGAA GAAGGCAGGC CGGCGTGGCC TGTGTGGCAC AATACTTATC 
CACAAGGTAG CAGGTGCTCT AGCTGAGGAA GGTATGGGGC TGGAGGAGAT CACAAAGAAG 
GTGAGCGTGA TCGCCAAGGC CATAGGTACT CTGGGGGTGA GCTTGTCTCC CTGCAGTGTC 
CCTGGTACCA AACCCACCTT TGAGCTTGCA GCTGATGAAA TGGAGCTAGG CCTGGGGATC 
CATGGGGAAG CCGGTGTTCG TCGGATAAAG CTGGTACCTG TTGATCAGAT TGTCACTCTT 
ATGCTTGACC ACATGACAGA CACCTCCAAT ATATCCCATG TGCCTGTGAA GTCAGGCTCT 
TCAGTGGTGC TGATGGTCAA CAATCTGGGT GGCCTGTCTT TCCTGGAACT GGGCATCATA 
GCTGATGCTG CCATACGCCT GCTGGAGGGC CGTGGGGTGA AGGTCGCCCG TGCCCTGGTG 
GGTACCTTCA TGTCAGCACT AGAGATGCGT GGTGTTTCCC TTACTTTGAT GCTTGTGGAT 
GAACCCCTGC TGAAGCTGAT AGATGCTGAA ACTAATGCAA AAGCCTGGCC TCACATGTCC 
AAGGTCTCTG TGACTGGAAG GAATCGGATT CGGGCAGCTC CCACAGAGCC TGCAGAAGCC 
CCTGAAGCCA CTGCAGCAGG AGGTGTAGCA TCAAAGCAGA TGACACTTGT GCTGGATCGG 
ATATCCACCA CGCTCATCGG ACTGGAGGAG CACCTGAATG CCCTGGACAG GGCTGCTGGT 
GATGGGGACT GTGGTTCTAC CCATAGCCGT GCTGCCAAAG CCATTCAGGG GTGGTTAAAG 
GAAGGCCCAA CTCCAGCCAG CCCTGCCCAG GTGCTCTCCA AACTGTCTGT CCTGCTGCTG 
GAGAAGATGG GAGGCTCATC TGGGGCGCTC TACGGCCTGT TCCTGACTGC AGCTGCCCAG 
CCTCTCAAGG CCAACACTGA CCTCCCAGCC TGGTCTGCTG CTATGGATGC CGGCCTAAAG 
GCCATGCAGA AGTATGGAAA GGCTGCACCA GGAGACAGGA CAATGCTGGA TTCTCTGTGG 
GCAGCAGCAC AGGAGCTCCA AGCCTGGAAG AGCCCAGGGG CCAGTCTCCT CCCAGTCCTG 
ACTAAAGCAG TCAAAAGTGC CGAAGCTGCA GCCGAGGCCA CCAAGAACAT GGAAGCTGGT 
GCTGGGAGAG CGAGTTACAT CAGCTCTGCA CAACTAGATC AGCCAGACCC TGGAGCAGTT 
GCGGCTGCAG CCATCTTCCG TGCCATCCTG GAGGTCTTGC AGACGAAGGC AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034120.1
CDS	280..2016
Translation	MSSKKMVNSVEGCAGDALAGFVACNPDLQLLQGYRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFASPAVGSILAAIRAVAQAGTAGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVVIEDDSAFTVLKKAGRRGLCGTILIHKVAGALAEEGMGLEEITKKVSVIAKAIGTLGVSLSPCSVPGTKPTFELAADEMELGLGIHGEAGVRRIKLVPVDQIVTLMLDHMTDTSNISHVPVKSGSSVVLMVNNLGGLSFLELGIIADAAIRLLEGRGVKVARALVGTFMSALEMRGVSLTLMLVDEPLLKLIDAETNAKAWPHMSKVSVTGRNRIRAAPTEPAEAPEATAAGGVASKQMTLVLDRISTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLKEGPTPASPAQVLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKANTDLPAWSAAMDAGLKAMQKYGKAAPGDRTMLDSLWAAAQELQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAVAAAAIFRAILEVLQTKAA

Target ORF information:

RefSeq Version	NM_001039031.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus triokinase and FMN cyclase (Tkfc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039031.1

ORF Insert Sequence:

ATGAGCTCCA AGAAGATGGT GAATTCTGTG GAAGGGTGTG CTGGTGATGC CCTTGCTGGG 
TTTGTTGCCT GTAACCCTGA CTTGCAGCTC CTGCAAGGGT ACCGTGTAGC CCTACGTTCT 
GACCTGGACA GCCTCAAGGG CCGGGTGGCA CTTCTGTCAG GTGGAGGCTC AGGCCATGAG 
CCTGCCCATG CCGGTTTTAT CGGGAAAGGA ATGCTGACAG GTGTCATTGC AGGAGCAGTG 
TTTGCCTCTC CTGCTGTGGG CAGCATCCTG GCAGCCATTA GAGCTGTGGC CCAGGCAGGC 
ACAGCCGGCA CCCTCCTCAT CGTGAAGAAC TACACTGGGG ATCGGCTCAA CTTTGGACTT 
GCCATGGAGC AGGCCAAGGC CGAGGGCATC TCTGTGGAGA TGGTGGTAAT TGAGGATGAC 
AGCGCCTTCA CTGTCCTGAA GAAGGCAGGC CGGCGTGGCC TGTGTGGCAC AATACTTATC 
CACAAGGTAG CAGGTGCTCT AGCTGAGGAA GGTATGGGGC TGGAGGAGAT CACAAAGAAG 
GTGAGCGTGA TCGCCAAGGC CATAGGTACT CTGGGGGTGA GCTTGTCTCC CTGCAGTGTC 
CCTGGTACCA AACCCACCTT TGAGCTTGCA GCTGATGAAA TGGAGCTAGG CCTGGGGATC 
CATGGGGAAG CCGGTGTTCG TCGGATAAAG CTGGTACCTG TTGATCAGAT TGTCACTCTT 
ATGCTTGACC ACATGACAGA CACCTCCAAT ATATCCCATG TGCCTGTGAA GTCAGGCTCT 
TCAGTGGTGC TGATGGTCAA CAATCTGGGT GGCCTGTCTT TCCTGGAACT GGGCATCATA 
GCTGATGCTG CCATACGCCT GCTGGAGGGC CGTGGGGTGA AGGTCGCCCG TGCCCTGGTG 
GGTACCTTCA TGTCAGCACT AGAGATGCGT GGTGTTTCCC TTACTTTGAT GCTTGTGGAT 
GAACCCCTGC TGAAGCTGAT AGATGCTGAA ACTAATGCAA AAGCCTGGCC TCACATGTCC 
AAGGTCTCTG TGACTGGAAG GAATCGGATT CGGGCAGCTC CCACAGAGCC TGCAGAAGCC 
CCTGAAGCCA CTGCAGCAGG AGGTGTAGCA TCAAAGCAGA TGACACTTGT GCTGGATCGG 
ATATCCACCA CGCTCATCGG ACTGGAGGAG CACCTGAATG CCCTGGACAG GGCTGCTGGT 
GATGGGGACT GTGGTTCTAC CCATAGCCGT GCTGCCAAAG CCATTCAGGG GTGGTTAAAG 
GAAGGCCCAA CTCCAGCCAG CCCTGCCCAG GTGCTCTCCA AACTGTCTGT CCTGCTGCTG 
GAGAAGATGG GAGGCTCATC TGGGGCGCTC TACGGCCTGT TCCTGACTGC AGCTGCCCAG 
CCTCTCAAGG CCAACACTGA CCTCCCAGCC TGGTCTGCTG CTATGGATGC CGGCCTAAAG 
GCCATGCAGA AGTATGGAAA GGCTGCACCA GGAGACAGGA CAATGCTGGA TTCTCTGTGG 
GCAGCAGCAC AGGAGCTCCA AGCCTGGAAG AGCCCAGGGG CCAGTCTCCT CCCAGTCCTG 
ACTAAAGCAG TCAAAAGTGC CGAAGCTGCA GCCGAGGCCA CCAAGAACAT GGAAGCTGGT 
GCTGGGAGAG CGAGTTACAT CAGCTCTGCA CAACTAGATC AGCCAGACCC TGGAGCAGTT 
GCGGCTGCAG CCATCTTCCG TGCCATCCTG GAGGTCTTGC AGACGAAGGC AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tkfc ( XM_006231056.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231118.1 Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa08254
Clone ID Related Accession (Same CDS sequence)	XM_006231056.3 , NM_001039031.1
Accession Version	XM_006231056.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	triokinase/FMN cyclase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCA AGAAGATGGT GAATTCTGTG GAAGGGTGTG CTGGTGATGC CCTTGCTGGG 
TTTGTTGCCT GTAACCCTGA CTTGCAGCTC CTGCAAGGGT ACCGTGTAGC CCTACGTTCT 
GACCTGGACA GCCTCAAGGG CCGGGTGGCA CTTCTGTCAG GTGGAGGCTC AGGCCATGAG 
CCTGCCCATG CCGGTTTTAT CGGGAAAGGA ATGCTGACAG GTGTCATTGC AGGAGCAGTG 
TTTGCCTCTC CTGCTGTGGG CAGCATCCTG GCAGCCATTA GAGCTGTGGC CCAGGCAGGC 
ACAGCCGGCA CCCTCCTCAT CGTGAAGAAC TACACTGGGG ATCGGCTCAA CTTTGGACTT 
GCCATGGAGC AGGCCAAGGC CGAGGGCATC TCTGTGGAGA TGGTGGTAAT TGAGGATGAC 
AGCGCCTTCA CTGTCCTGAA GAAGGCAGGC CGGCGTGGCC TGTGTGGCAC AATACTTATC 
CACAAGGTAG CAGGTGCTCT AGCTGAGGAA GGTATGGGGC TGGAGGAGAT CACAAAGAAG 
GTGAGCGTGA TCGCCAAGGC CATAGGTACT CTGGGGGTGA GCTTGTCTCC CTGCAGTGTC 
CCTGGTACCA AACCCACCTT TGAGCTTGCA GCTGATGAAA TGGAGCTAGG CCTGGGGATC 
CATGGGGAAG CCGGTGTTCG TCGGATAAAG CTGGTACCTG TTGATCAGAT TGTCACTCTT 
ATGCTTGACC ACATGACAGA CACCTCCAAT ATATCCCATG TGCCTGTGAA GTCAGGCTCT 
TCAGTGGTGC TGATGGTCAA CAATCTGGGT GGCCTGTCTT TCCTGGAACT GGGCATCATA 
GCTGATGCTG CCATACGCCT GCTGGAGGGC CGTGGGGTGA AGGTCGCCCG TGCCCTGGTG 
GGTACCTTCA TGTCAGCACT AGAGATGCGT GGTGTTTCCC TTACTTTGAT GCTTGTGGAT 
GAACCCCTGC TGAAGCTGAT AGATGCTGAA ACTAATGCAA AAGCCTGGCC TCACATGTCC 
AAGGTCTCTG TGACTGGAAG GAATCGGATT CGGGCAGCTC CCACAGAGCC TGCAGAAGCC 
CCTGAAGCCA CTGCAGCAGG AGGTGTAGCA TCAAAGCAGA TGACACTTGT GCTGGATCGG 
ATATCCACCA CGCTCATCGG ACTGGAGGAG CACCTGAATG CCCTGGACAG GGCTGCTGGT 
GATGGGGACT GTGGTTCTAC CCATAGCCGT GCTGCCAAAG CCATTCAGGG GTGGTTAAAG 
GAAGGCCCAA CTCCAGCCAG CCCTGCCCAG GTGCTCTCCA AACTGTCTGT CCTGCTGCTG 
GAGAAGATGG GAGGCTCATC TGGGGCGCTC TACGGCCTGT TCCTGACTGC AGCTGCCCAG 
CCTCTCAAGG CCAACACTGA CCTCCCAGCC TGGTCTGCTG CTATGGATGC CGGCCTAAAG 
GCCATGCAGA AGTATGGAAA GGCTGCACCA GGAGACAGGA CAATGCTGGA TTCTCTGTGG 
GCAGCAGCAC AGGAGCTCCA AGCCTGGAAG AGCCCAGGGG CCAGTCTCCT CCCAGTCCTG 
ACTAAAGCAG TCAAAAGTGC CGAAGCTGCA GCCGAGGCCA CCAAGAACAT GGAAGCTGGT 
GCTGGGAGAG CGAGTTACAT CAGCTCTGCA CAACTAGATC AGCCAGACCC TGGAGCAGTT 
GCGGCTGCAG CCATCTTCCG TGCCATCCTG GAGGTCTTGC AGACGAAGGC AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231118.1
CDS	193..1929
Translation	MSSKKMVNSVEGCAGDALAGFVACNPDLQLLQGYRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFASPAVGSILAAIRAVAQAGTAGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVVIEDDSAFTVLKKAGRRGLCGTILIHKVAGALAEEGMGLEEITKKVSVIAKAIGTLGVSLSPCSVPGTKPTFELAADEMELGLGIHGEAGVRRIKLVPVDQIVTLMLDHMTDTSNISHVPVKSGSSVVLMVNNLGGLSFLELGIIADAAIRLLEGRGVKVARALVGTFMSALEMRGVSLTLMLVDEPLLKLIDAETNAKAWPHMSKVSVTGRNRIRAAPTEPAEAPEATAAGGVASKQMTLVLDRISTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLKEGPTPASPAQVLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKANTDLPAWSAAMDAGLKAMQKYGKAAPGDRTMLDSLWAAAQELQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAVAAAAIFRAILEVLQTKAA

Target ORF information:

RefSeq Version	XM_006231056.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231056.3

ORF Insert Sequence:

ATGAGCTCCA AGAAGATGGT GAATTCTGTG GAAGGGTGTG CTGGTGATGC CCTTGCTGGG 
TTTGTTGCCT GTAACCCTGA CTTGCAGCTC CTGCAAGGGT ACCGTGTAGC CCTACGTTCT 
GACCTGGACA GCCTCAAGGG CCGGGTGGCA CTTCTGTCAG GTGGAGGCTC AGGCCATGAG 
CCTGCCCATG CCGGTTTTAT CGGGAAAGGA ATGCTGACAG GTGTCATTGC AGGAGCAGTG 
TTTGCCTCTC CTGCTGTGGG CAGCATCCTG GCAGCCATTA GAGCTGTGGC CCAGGCAGGC 
ACAGCCGGCA CCCTCCTCAT CGTGAAGAAC TACACTGGGG ATCGGCTCAA CTTTGGACTT 
GCCATGGAGC AGGCCAAGGC CGAGGGCATC TCTGTGGAGA TGGTGGTAAT TGAGGATGAC 
AGCGCCTTCA CTGTCCTGAA GAAGGCAGGC CGGCGTGGCC TGTGTGGCAC AATACTTATC 
CACAAGGTAG CAGGTGCTCT AGCTGAGGAA GGTATGGGGC TGGAGGAGAT CACAAAGAAG 
GTGAGCGTGA TCGCCAAGGC CATAGGTACT CTGGGGGTGA GCTTGTCTCC CTGCAGTGTC 
CCTGGTACCA AACCCACCTT TGAGCTTGCA GCTGATGAAA TGGAGCTAGG CCTGGGGATC 
CATGGGGAAG CCGGTGTTCG TCGGATAAAG CTGGTACCTG TTGATCAGAT TGTCACTCTT 
ATGCTTGACC ACATGACAGA CACCTCCAAT ATATCCCATG TGCCTGTGAA GTCAGGCTCT 
TCAGTGGTGC TGATGGTCAA CAATCTGGGT GGCCTGTCTT TCCTGGAACT GGGCATCATA 
GCTGATGCTG CCATACGCCT GCTGGAGGGC CGTGGGGTGA AGGTCGCCCG TGCCCTGGTG 
GGTACCTTCA TGTCAGCACT AGAGATGCGT GGTGTTTCCC TTACTTTGAT GCTTGTGGAT 
GAACCCCTGC TGAAGCTGAT AGATGCTGAA ACTAATGCAA AAGCCTGGCC TCACATGTCC 
AAGGTCTCTG TGACTGGAAG GAATCGGATT CGGGCAGCTC CCACAGAGCC TGCAGAAGCC 
CCTGAAGCCA CTGCAGCAGG AGGTGTAGCA TCAAAGCAGA TGACACTTGT GCTGGATCGG 
ATATCCACCA CGCTCATCGG ACTGGAGGAG CACCTGAATG CCCTGGACAG GGCTGCTGGT 
GATGGGGACT GTGGTTCTAC CCATAGCCGT GCTGCCAAAG CCATTCAGGG GTGGTTAAAG 
GAAGGCCCAA CTCCAGCCAG CCCTGCCCAG GTGCTCTCCA AACTGTCTGT CCTGCTGCTG 
GAGAAGATGG GAGGCTCATC TGGGGCGCTC TACGGCCTGT TCCTGACTGC AGCTGCCCAG 
CCTCTCAAGG CCAACACTGA CCTCCCAGCC TGGTCTGCTG CTATGGATGC CGGCCTAAAG 
GCCATGCAGA AGTATGGAAA GGCTGCACCA GGAGACAGGA CAATGCTGGA TTCTCTGTGG 
GCAGCAGCAC AGGAGCTCCA AGCCTGGAAG AGCCCAGGGG CCAGTCTCCT CCCAGTCCTG 
ACTAAAGCAG TCAAAAGTGC CGAAGCTGCA GCCGAGGCCA CCAAGAACAT GGAAGCTGGT 
GCTGGGAGAG CGAGTTACAT CAGCTCTGCA CAACTAGATC AGCCAGACCC TGGAGCAGTT 
GCGGCTGCAG CCATCTTCCG TGCCATCCTG GAGGTCTTGC AGACGAAGGC AGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tkfc ( XM_006231057.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231119.2 Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47998
Clone ID Related Accession (Same CDS sequence)	XM_006231057.2
Accession Version	XM_006231057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	triokinase/FMN cyclase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACGA GCGAAGAGAC TGAGAACCCC CACCCTCACG CAGCCAACTC CGTGGCTGTT 
GCCTCCGCCC CTAAGACACG TTGCAGGCCA GAGAGGCCAG GCGCAGAGGA TCACGGGACA 
GCCTCACCTG GCCTCTTGGA GGACTTACGG AGCCCGAGGC GGCCAACCAA GCGAGGCCCC 
GCCCCTGAGG CCCCGCCTTT TCCTAACCGC CAGAGGCCTC AGTACCCGGA TGAGAGCGAG 
AGTTCGGGTA CGCAGACTAA ACCGGCCTCC ACTTTGGTCA ATTACGCCCT AAGAGGCCGC 
CTGCTGGTCC CCCTGCCACG GTGCTGCTGC CGTCTCTACT TCAGACCAGT TAGCAGCAGT 
CAGGACTGTC AACCCTGCAG CAACTCAGAG CAGAAGTGTG AGCTCGACTT GTTTCGAAGC 
TTTCCCACCA TGAGCTCCAA GAAGATGGTG AATTCTGTGG AAGGGTGTGC TGGTGATGCC 
CTTGCTGGGT TTGTTGCCTG TAACCCTGAC TTGCAGCTCC TGCAAGGGTA CCGTGTAGCC 
CTACGTTCTG ACCTGGACAG CCTCAAGGGC CGGGTGGCAC TTCTGTCAGG TGGAGGCTCA 
GGCCATGAGC CTGCCCATGC CGGTTTTATC GGGAAAGGAA TGCTGACAGG TGTCATTGCA 
GGAGCAGTGT TTGCCTCTCC TGCTGTGGGC AGCATCCTGG CAGCCATTAG AGCTGTGGCC 
CAGGCAGGCA CAGCCGGCAC CCTCCTCATC GTGAAGAACT ACACTGGGGA TCGGCTCAAC 
TTTGGACTTG CCATGGAGCA GGCCAAGGCC GAGGGCATCT CTGTGGAGAT GGTGGTAATT 
GAGGATGACA GCGCCTTCAC TGTCCTGAAG AAGGCAGGCC GGCGTGGCCT GTGTGGCACA 
ATACTTATCC ACAAGGTAGC AGGTGCTCTA GCTGAGGAAG GTATGGGGCT GGAGGAGATC 
ACAAAGAAGG TGAGCGTGAT CGCCAAGGCC ATAGGTACTC TGGGGGTGAG CTTGTCTCCC 
TGCAGTGTCC CTGGTACCAA ACCCACCTTT GAGCTTGCAG CTGATGAAAT GGAGCTAGGC 
CTGGGGATCC ATGGGGAAGC CGGTGTTCGT CGGATAAAGC TGGTACCTGT TGATCAGATT 
GTCACTCTTA TGCTTGACCA CATGACAGAC ACCTCCAATA TATCCCATGT GCCTGTGAAG 
TCAGGCTCTT CAGTGGTGCT GATGGTCAAC AATCTGGGTG GCCTGTCTTT CCTGGAACTG 
GGCATCATAG CTGATGCTGC CATACGCCTG CTGGAGGGCC GTGGGGTGAA GGTCGCCCGT 
GCCCTGGTGG GTACCTTCAT GTCAGCACTA GAGATGCGTG GTGTTTCCCT TACTTTGATG 
CTTGTGGATG AACCCCTGCT GAAGCTGATA GATGCTGAAA CTAATGCAAA AGCCTGGCCT 
CACATGTCCA AGGTCTCTGT GACTGGAAGG AATCGGATTC GGGCAGCTCC CACAGAGCCT 
GCAGAAGCCC CTGAAGCCAC TGCAGCAGGA GGTGTAGCAT CAAAGCAGAT GACACTTGTG 
CTGGATCGGA TATCCACCAC GCTCATCGGA CTGGAGGAGC ACCTGAATGC CCTGGACAGG 
GCTGCTGGTG ATGGGGACTG TGGTTCTACC CATAGCCGTG CTGCCAAAGC CATTCAGGGG 
TGGTTAAAGG AAGGCCCAAC TCCAGCCAGC CCTGCCCAGG TGCTCTCCAA ACTGTCTGTC 
CTGCTGCTGG AGAAGATGGG AGGCTCATCT GGGGCGCTCT ACGGCCTGTT CCTGACTGCA 
GCTGCCCAGC CTCTCAAGGC CAACACTGAC CTCCCAGCCT GGTCTGCTGC TATGGATGCC 
GGCCTAAAGG CCATGCAGAA GTATGGAAAG GCTGCACCAG GAGACAGGAC AATGCTGGAT 
TCTCTGTGGG CAGCAGCACA GGAGCTCCAA GCCTGGAAGA GCCCAGGGGC CAGTCTCCTC 
CCAGTCCTGA CTAAAGCAGT CAAAAGTGCC GAAGCTGCAG CCGAGGCCAC CAAGAACATG 
GAAGCTGGTG CTGGGAGAGC GAGTTACATC AGCTCTGCAC AACTAGATCA GCCAGACCCT 
GGAGCAGTTG CGGCTGCAGC CATCTTCCGT GCCATCCTGG AGGTCTTGCA GACGAAGGCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231119.2
CDS	20..2185
Translation	MGTSEETENPHPHAANSVAVASAPKTRCRPERPGAEDHGTASPGLLEDLRSPRRPTKRGPAPEAPPFPNRQRPQYPDESESSGTQTKPASTLVNYALRGRLLVPLPRCCCRLYFRPVSSSQDCQPCSNSEQKCELDLFRSFPTMSSKKMVNSVEGCAGDALAGFVACNPDLQLLQGYRVALRSDLDSLKGRVALLSGGGSGHEPAHAGFIGKGMLTGVIAGAVFASPAVGSILAAIRAVAQAGTAGTLLIVKNYTGDRLNFGLAMEQAKAEGISVEMVVIEDDSAFTVLKKAGRRGLCGTILIHKVAGALAEEGMGLEEITKKVSVIAKAIGTLGVSLSPCSVPGTKPTFELAADEMELGLGIHGEAGVRRIKLVPVDQIVTLMLDHMTDTSNISHVPVKSGSSVVLMVNNLGGLSFLELGIIADAAIRLLEGRGVKVARALVGTFMSALEMRGVSLTLMLVDEPLLKLIDAETNAKAWPHMSKVSVTGRNRIRAAPTEPAEAPEATAAGGVASKQMTLVLDRISTTLIGLEEHLNALDRAAGDGDCGSTHSRAAKAIQGWLKEGPTPASPAQVLSKLSVLLLEKMGGSSGALYGLFLTAAAQPLKANTDLPAWSAAMDAGLKAMQKYGKAAPGDRTMLDSLWAAAQELQAWKSPGASLLPVLTKAVKSAEAAAEATKNMEAGAGRASYISSAQLDQPDPGAVAAAAIFRAILEVLQTKAA

Target ORF information:

RefSeq Version	XM_006231057.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus triokinase and FMN cyclase (Tkfc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231057.2

ORF Insert Sequence:

ATGGGCACGA GCGAAGAGAC TGAGAACCCC CACCCTCACG CAGCCAACTC CGTGGCTGTT 
GCCTCCGCCC CTAAGACACG TTGCAGGCCA GAGAGGCCAG GCGCAGAGGA TCACGGGACA 
GCCTCACCTG GCCTCTTGGA GGACTTACGG AGCCCGAGGC GGCCAACCAA GCGAGGCCCC 
GCCCCTGAGG CCCCGCCTTT TCCTAACCGC CAGAGGCCTC AGTACCCGGA TGAGAGCGAG 
AGTTCGGGTA CGCAGACTAA ACCGGCCTCC ACTTTGGTCA ATTACGCCCT AAGAGGCCGC 
CTGCTGGTCC CCCTGCCACG GTGCTGCTGC CGTCTCTACT TCAGACCAGT TAGCAGCAGT 
CAGGACTGTC AACCCTGCAG CAACTCAGAG CAGAAGTGTG AGCTCGACTT GTTTCGAAGC 
TTTCCCACCA TGAGCTCCAA GAAGATGGTG AATTCTGTGG AAGGGTGTGC TGGTGATGCC 
CTTGCTGGGT TTGTTGCCTG TAACCCTGAC TTGCAGCTCC TGCAAGGGTA CCGTGTAGCC 
CTACGTTCTG ACCTGGACAG CCTCAAGGGC CGGGTGGCAC TTCTGTCAGG TGGAGGCTCA 
GGCCATGAGC CTGCCCATGC CGGTTTTATC GGGAAAGGAA TGCTGACAGG TGTCATTGCA 
GGAGCAGTGT TTGCCTCTCC TGCTGTGGGC AGCATCCTGG CAGCCATTAG AGCTGTGGCC 
CAGGCAGGCA CAGCCGGCAC CCTCCTCATC GTGAAGAACT ACACTGGGGA TCGGCTCAAC 
TTTGGACTTG CCATGGAGCA GGCCAAGGCC GAGGGCATCT CTGTGGAGAT GGTGGTAATT 
GAGGATGACA GCGCCTTCAC TGTCCTGAAG AAGGCAGGCC GGCGTGGCCT GTGTGGCACA 
ATACTTATCC ACAAGGTAGC AGGTGCTCTA GCTGAGGAAG GTATGGGGCT GGAGGAGATC 
ACAAAGAAGG TGAGCGTGAT CGCCAAGGCC ATAGGTACTC TGGGGGTGAG CTTGTCTCCC 
TGCAGTGTCC CTGGTACCAA ACCCACCTTT GAGCTTGCAG CTGATGAAAT GGAGCTAGGC 
CTGGGGATCC ATGGGGAAGC CGGTGTTCGT CGGATAAAGC TGGTACCTGT TGATCAGATT 
GTCACTCTTA TGCTTGACCA CATGACAGAC ACCTCCAATA TATCCCATGT GCCTGTGAAG 
TCAGGCTCTT CAGTGGTGCT GATGGTCAAC AATCTGGGTG GCCTGTCTTT CCTGGAACTG 
GGCATCATAG CTGATGCTGC CATACGCCTG CTGGAGGGCC GTGGGGTGAA GGTCGCCCGT 
GCCCTGGTGG GTACCTTCAT GTCAGCACTA GAGATGCGTG GTGTTTCCCT TACTTTGATG 
CTTGTGGATG AACCCCTGCT GAAGCTGATA GATGCTGAAA CTAATGCAAA AGCCTGGCCT 
CACATGTCCA AGGTCTCTGT GACTGGAAGG AATCGGATTC GGGCAGCTCC CACAGAGCCT 
GCAGAAGCCC CTGAAGCCAC TGCAGCAGGA GGTGTAGCAT CAAAGCAGAT GACACTTGTG 
CTGGATCGGA TATCCACCAC GCTCATCGGA CTGGAGGAGC ACCTGAATGC CCTGGACAGG 
GCTGCTGGTG ATGGGGACTG TGGTTCTACC CATAGCCGTG CTGCCAAAGC CATTCAGGGG 
TGGTTAAAGG AAGGCCCAAC TCCAGCCAGC CCTGCCCAGG TGCTCTCCAA ACTGTCTGTC 
CTGCTGCTGG AGAAGATGGG AGGCTCATCT GGGGCGCTCT ACGGCCTGTT CCTGACTGCA 
GCTGCCCAGC CTCTCAAGGC CAACACTGAC CTCCCAGCCT GGTCTGCTGC TATGGATGCC 
GGCCTAAAGG CCATGCAGAA GTATGGAAAG GCTGCACCAG GAGACAGGAC AATGCTGGAT 
TCTCTGTGGG CAGCAGCACA GGAGCTCCAA GCCTGGAAGA GCCCAGGGGC CAGTCTCCTC 
CCAGTCCTGA CTAAAGCAGT CAAAAGTGCC GAAGCTGCAG CCGAGGCCAC CAAGAACATG 
GAAGCTGGTG CTGGGAGAGC GAGTTACATC AGCTCTGCAC AACTAGATCA GCCAGACCCT 
GGAGCAGTTG CGGCTGCAGC CATCTTCCGT GCCATCCTGG AGGTCTTGCA GACGAAGGCA 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements.
The Journal of biological chemistry289(15)10620-36(2014 Apr)
Rodrigues JR,Couto A,Cabezas A,Pinto RM,Ribeiro JM,Canales J,Costas MJ,Cameselle JC

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Negative regulation of MDA5- but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase.
Proceedings of the National Academy of Sciences of the United States of America104(28)11706-11(2007 Jul)
Diao F,Li S,Tian Y,Zhang M,Xu LG,Zhang Y,Wang RP,Chen D,Zhai Z,Zhong B,Tien P,Shu HB

Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases.
Biochemical and biophysical research communications338(4)1682-9(2005 Dec)
Cabezas A,Costas MJ,Pinto RM,Couto A,Cameselle JC

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Tkfc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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