Cyp2b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp2b2
Entrez Gene ID	361523
Full Name	cytochrome P450, family 2, subfamily b, polypeptide 2
Synonyms	CYPIIB2,Cype
General protein information	Preferred Names cytochrome P450, family 2, subfamily b, polypeptide 2 Names cytochrome P450 2B2 cytochrome P-450b type e cytochrome P-450e-M cytochrome P450 PB4 cytochrome P450, subfamily IIB (phenobarbital-inducible)(b, e) cytochrome P450E
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	phenobarbital-inducible member of the p450 xenobiotic-inducible superfamily that metabolizes nicotine and some drugs and activates carcinogens [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198676.1	NP_001185605.1	cytochrome P450 2B2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyp2b10	NP_034129.1
	CYP2B6	NP_001035302.1
Homo sapiens (human)	CYP2B6	NP_000758.1
Rattus norvegicus (Norway rat)	Cyp2b1	NP_001128316.1
Bos taurus (cattle)	CYP2B6	NP_001068641.1
Rattus norvegicus (Norway rat)	Cyp2b2	NP_001185605.1
Pan troglodytes (chimpanzee)	LOC456052	XP_003316405.1
Canis lupus familiaris (dog)	CYP2B6	NP_001006653.1

Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide-dependent CYP2B degradation is potentiated by a cytokine-regulated pathway and utilizes the immunoproteasome subunit LMP2.
Sun H, Lee CM, Tripathi S, Kim KB, Morgan ET
The Biochemical journal445(3)377-82(2012 Aug)

Peroxisome proliferator-activated receptor alpha (PPARalpha) agonists induce constitutive androstane receptor (CAR) and cytochrome P450 2B in rat primary hepatocytes.
Saito K, Kobayashi K, Mizuno Y, Fukuchi Y, Furihata T, Chiba K
Drug metabolism and pharmacokinetics25(1)108-11(2010)

A spectrophotometric method to monitor the catalytic activity of microsomal cytochrome P-450 IIB1/2: comparison with fluorometric assay.
Rastogi S, Khanna SK, Das M
Indian journal of biochemistry & biophysics39(3)191-6(2002 Jun)

Sequence requirements for cytochrome P-450IIB1 catalytic activity. Alteration of the stereospecificity and regioselectivity of steroid hydroxylation by a simultaneous change of two hydrophobic amino acid residues to phenylalanine.
Aoyama T, Korzekwa K, Nagata K, Adesnik M, Reiss A, Lapenson DP, Gillette J, Gelboin HV, Waxman DJ, Gonzalez FJ
The Journal of biological chemistry264(35)21327-33(1989 Dec)

Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450.
Pyerin W, Taniguchi H
The EMBO journal8(10)3003-10(1989 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

stimulation of hepatic heme oxygenase-1 expression by ascorbic acid deficiency caused the decrease in CYP content in liver
Title: Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats.

LMP2 contributes to the NO-dependent degradation of CYP2B proteins, and induction of LMP2 may be involved in the potentiation of this degradation by IL-1.
Title: Nitric oxide-dependent CYP2B degradation is potentiated by a cytokine-regulated pathway and utilizes the immunoproteasome subunit LMP2.

Werner syndrome protein WRN is recruited to the CYP2B2 promoter in response to phenobarbital treatment in rat liver chromatin.
Title: Werner's syndrome helicase participates in transcription of phenobarbital-inducible CYP2B genes in rat and mouse liver.

NanoString provides an additional new technology to evaluate the induction of CYP1A1 and 2B1/2, as well as potentially other enzymes or transporters in rat liver slices.
Title: Evaluation of CYP1A1 and CYP2B1/2 m-RNA induction in rat liver slices using the NanoString technology: a novel tool for drug discovery lead optimization.

analysis of the spectrophotometric method to monitor the catalytic activity of microsomal cytochrome P-450 IIB1/2 and comparison with fluorometric assay
Title: A spectrophotometric method to monitor the catalytic activity of microsomal cytochrome P-450 IIB1/2: comparison with fluorometric assay.

The following Cyp2b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp2b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08588	NM_001198676.1 Latest version!	Rattus norvegicus cytochrome P450, family 2, subfamily b, polypeptide 2 (Cyp2b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp2b2 ( NM_001198676.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001185605.1 Rattus norvegicus cytochrome P450, family 2, subfamily b, polypeptide 2 (Cyp2b2), mRNA.

Price & Availability↑

CloneID	ORa08588
Clone ID Related Accession (Same CDS sequence)	NM_001198676.1
Accession Version	NM_001198676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 2B2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07002749.1 and M34452.1. On or before Nov 4, 2010 this sequence version replaced XM_001070953.2, XM_001062335.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript and protein alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906355, SAMN01906356 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCA GTATCTTGCT CCTCCTTGCT CTCCTTGTGG GCTTCTTGTT ACTCTTAGTC 
AGGGGACACC CAAAGTCCCG TGGCAACTTC CCACCAGGAC CTCGTCCCCT TCCCCTCTTG 
GGGAACCTCC TGCAGTTGGA CAGAGGAGGC CTCCTCAATT CCTTCATGCA GTTTCGCGAA 
AAATATGGAG ATGTGTTCAC AGTACACCTG GGACCAAGGC CTGTGGTCAT GCTATGTGGG 
ACAGACACCA TAAAGGAGGC TCTGGTGGGC CAAGCTGAAG ATTTCTCTGG TCGGGGAACA 
ATCGCTGTGA TTGAGCCAAT CTTCAAGGAA TATGGTGTGT TCTTTGCCAA TGGGGAACGC 
TGGAAGGCCC TTCGGCGATT CTCTCTGGCT ACCATGAGAG ACTTTGGGAT GGGAAAGAGG 
AGTGTGGAAG AACGGATTCA GGAGGAAGCC CAATGTTTGG TGGAGGAACT GCGGAAATCC 
CAGGGAGCCC CACTGGATCC CACCTTCCTC TTCCAGTGCA TCACAGCCAA CATCATCTGC 
TCCATTGTGT TTGGAGAGCG CTTTGACTAC ACAGACCGCC AGTTCCTGCG CCTGTTGGAG 
CTGTTCTACC GGACCTTTTC CCTCCTAAGT TCATTCTCCA GCCAGGTGTT TGAGTTCTTC 
TCTGGGTTCC TGAAATACTT TCCTGGTGCC CACAGACAAA TCTCCAAAAA CCTCCAGGAA 
ATCCTCGATT ACATTGGCCA TATTGTGGAG AAGCACAGGG CCACCTTAGA CCCCAGCGCT 
CCACGAGACT TCATCGACAC TTACCTTCTG CGCATGGAGA AAGTGAGTCC TGCATGGATG 
AGAGAGGAGA AGTCGAACCA CCACACAGAG TTCCATCATG AGAACCTCAT GATCTCCCTG 
CTCTCTCTCT TCTTTGCTGG CACTGAGACC GGCAGCACCA CACTCCGCTA TGGTTTCCTG 
CTCATGCTCA AGTACCCCCA TGTCACAGAG AAAGTCCAAA AGGAGATTGA TCAGGTGATT 
GGCTCTCACA GGCCACCATC CCTTGATGAT CGTACCAAAA TGCCATACAC TGATGCAGTC 
ATCCACGAGA TTCAGAGATT TGCAGATCTT GCCCCAATTG GTTTACCACA CAGAGTCACC 
AAAGACACCA TGTTCCGAGG GTACCTGCTC CCCAAGAACA CTGAGGTGTA TCCCATCCTG 
AGTTCAGCTC TCCATGACCC ACAGTACTTT GACCATCCAG ACACCTTCAA TCCTGAGCAC 
TTCCTGGATG CCGATGGGAC ACTGAAAAAG AGTGAAGCTT TTATGCCCTT CTCCACAGGA 
AAGCGCATTT GTCTTGGCGA AGGCATTGCC CGAAATGAAT TGTTCCTCTT CTTCACCACC 
ATCCTCCAGA ACTTCTCTGT GTCAAGCCAT TTGGCTCCCA AGGACATTGA CCTCACGCCC 
AAGGAGAGTG GCATTGCAAA AATACCTCCA ACATACCAGA TCTGCTTCTC AGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185605.1
CDS	1..1500
Translation	MEPSILLLLALLVGFLLLLVRGHPKSRGNFPPGPRPLPLLGNLLQLDRGGLLNSFMQFREKYGDVFTVHLGPRPVVMLCGTDTIKEALVGQAEDFSGRGTIAVIEPIFKEYGVFFANGERWKALRRFSLATMRDFGMGKRSVEERIQEEAQCLVEELRKSQGAPLDPTFLFQCITANIICSIVFGERFDYTDRQFLRLLELFYRTFSLLSSFSSQVFEFFSGFLKYFPGAHRQISKNLQEILDYIGHIVEKHRATLDPSAPRDFIDTYLLRMEKVSPAWMREEKSNHHTEFHHENLMISLLSLFFAGTETGSTTLRYGFLLMLKYPHVTEKVQKEIDQVIGSHRPPSLDDRTKMPYTDAVIHEIQRFADLAPIGLPHRVTKDTMFRGYLLPKNTEVYPILSSALHDPQYFDHPDTFNPEHFLDADGTLKKSEAFMPFSTGKRICLGEGIARNELFLFFTTILQNFSVSSHLAPKDIDLTPKESGIAKIPPTYQICFSAR

Target ORF information:

RefSeq Version	NM_001198676.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 2, subfamily b, polypeptide 2 (Cyp2b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198676.1

ORF Insert Sequence:

ATGGAGCCCA GTATCTTGCT CCTCCTTGCT CTCCTTGTGG GCTTCTTGTT ACTCTTAGTC 
AGGGGACACC CAAAGTCCCG TGGCAACTTC CCACCAGGAC CTCGTCCCCT TCCCCTCTTG 
GGGAACCTCC TGCAGTTGGA CAGAGGAGGC CTCCTCAATT CCTTCATGCA GTTTCGCGAA 
AAATATGGAG ATGTGTTCAC AGTACACCTG GGACCAAGGC CTGTGGTCAT GCTATGTGGG 
ACAGACACCA TAAAGGAGGC TCTGGTGGGC CAAGCTGAAG ATTTCTCTGG TCGGGGAACA 
ATCGCTGTGA TTGAGCCAAT CTTCAAGGAA TATGGTGTGT TCTTTGCCAA TGGGGAACGC 
TGGAAGGCCC TTCGGCGATT CTCTCTGGCT ACCATGAGAG ACTTTGGGAT GGGAAAGAGG 
AGTGTGGAAG AACGGATTCA GGAGGAAGCC CAATGTTTGG TGGAGGAACT GCGGAAATCC 
CAGGGAGCCC CACTGGATCC CACCTTCCTC TTCCAGTGCA TCACAGCCAA CATCATCTGC 
TCCATTGTGT TTGGAGAGCG CTTTGACTAC ACAGACCGCC AGTTCCTGCG CCTGTTGGAG 
CTGTTCTACC GGACCTTTTC CCTCCTAAGT TCATTCTCCA GCCAGGTGTT TGAGTTCTTC 
TCTGGGTTCC TGAAATACTT TCCTGGTGCC CACAGACAAA TCTCCAAAAA CCTCCAGGAA 
ATCCTCGATT ACATTGGCCA TATTGTGGAG AAGCACAGGG CCACCTTAGA CCCCAGCGCT 
CCACGAGACT TCATCGACAC TTACCTTCTG CGCATGGAGA AAGTGAGTCC TGCATGGATG 
AGAGAGGAGA AGTCGAACCA CCACACAGAG TTCCATCATG AGAACCTCAT GATCTCCCTG 
CTCTCTCTCT TCTTTGCTGG CACTGAGACC GGCAGCACCA CACTCCGCTA TGGTTTCCTG 
CTCATGCTCA AGTACCCCCA TGTCACAGAG AAAGTCCAAA AGGAGATTGA TCAGGTGATT 
GGCTCTCACA GGCCACCATC CCTTGATGAT CGTACCAAAA TGCCATACAC TGATGCAGTC 
ATCCACGAGA TTCAGAGATT TGCAGATCTT GCCCCAATTG GTTTACCACA CAGAGTCACC 
AAAGACACCA TGTTCCGAGG GTACCTGCTC CCCAAGAACA CTGAGGTGTA TCCCATCCTG 
AGTTCAGCTC TCCATGACCC ACAGTACTTT GACCATCCAG ACACCTTCAA TCCTGAGCAC 
TTCCTGGATG CCGATGGGAC ACTGAAAAAG AGTGAAGCTT TTATGCCCTT CTCCACAGGA 
AAGCGCATTT GTCTTGGCGA AGGCATTGCC CGAAATGAAT TGTTCCTCTT CTTCACCACC 
ATCCTCCAGA ACTTCTCTGT GTCAAGCCAT TTGGCTCCCA AGGACATTGA CCTCACGCCC 
AAGGAGAGTG GCATTGCAAA AATACCTCCA ACATACCAGA TCTGCTTCTC AGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide-dependent CYP2B degradation is potentiated by a cytokine-regulated pathway and utilizes the immunoproteasome subunit LMP2.
The Biochemical journal445(3)377-82(2012 Aug)
Sun H,Lee CM,Tripathi S,Kim KB,Morgan ET

Peroxisome proliferator-activated receptor alpha (PPARalpha) agonists induce constitutive androstane receptor (CAR) and cytochrome P450 2B in rat primary hepatocytes.
Drug metabolism and pharmacokinetics25(1)108-11(2010)
Saito K,Kobayashi K,Mizuno Y,Fukuchi Y,Furihata T,Chiba K

A spectrophotometric method to monitor the catalytic activity of microsomal cytochrome P-450 IIB1/2: comparison with fluorometric assay.
Indian journal of biochemistry & biophysics39(3)191-6(2002 Jun)
Rastogi S,Khanna SK,Das M

Sequence requirements for cytochrome P-450IIB1 catalytic activity. Alteration of the stereospecificity and regioselectivity of steroid hydroxylation by a simultaneous change of two hydrophobic amino acid residues to phenylalanine.
The Journal of biological chemistry264(35)21327-33(1989 Dec)
Aoyama T,Korzekwa K,Nagata K,Adesnik M,Reiss A,Lapenson DP,Gillette J,Gelboin HV,Waxman DJ,Gonzalez FJ

Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450.
The EMBO journal8(10)3003-10(1989 Oct)
Pyerin W,Taniguchi H

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Cyp2b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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