Man2b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Man2b1
Entrez Gene ID	361378
Full Name	mannosidase, alpha, class 2B, member 1
Synonyms	Manb
General protein information	Preferred Names mannosidase, alpha, class 2B, member 1 Names lysosomal alpha-mannosidase mannosidase 2, alpha B1 mannosidase, alpha B, lysosomal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	mouse homolog is a lysosomal alpha-mannosidase which cleaves alpha-linked mannose residues on glycoproteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_199404.1	NP_955436.1	lysosomal alpha-mannosidase precursor
XM_006255273.3	XP_006255335.1	lysosomal alpha-mannosidase isoform X4
XM_017601312.1	XP_017456801.1	lysosomal alpha-mannosidase isoform X2
XM_017601313.1	XP_017456802.1	lysosomal alpha-mannosidase isoform X3
XM_017601311.1	XP_017456800.1	lysosomal alpha-mannosidase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B1	XP_003316198.2
Arabidopsis thaliana (thale cress)	AT5G13980	NP_196902.2
Bos taurus (cattle)	MAN2B1	NP_776986.2
Rattus norvegicus (Norway rat)	Man2b1	NP_955436.1
Mus musculus (house mouse)	Man2b1	NP_034894.2
Drosophila melanogaster (fruit fly)	LM408	NP_609408.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008584	XP_557731.3
Xenopus tropicalis (tropical clawed frog)	man2b1	NP_001072469.1
Homo sapiens (human)	MAN2B1	NP_000519.2
	LOC716173	XP_002801133.1
Canis lupus familiaris (dog)	MAN2B1	XP_005632890.1
Danio rerio (zebrafish)	man2b1	NP_001014354.1
Caenorhabditis elegans (roundworm)	aman-1	NP_508811.1
Oryza sativa (rice)	Os11g0525600	NP_001067993.1

Gene Ontology
Bibliography

Related articles in PubMed

The effects of swainsonine on the activity and expression of ?-mannosidase in BRL-3A cells.
Lu H, Ma F, Wang H, Geng PS, Wang SS, Wang JG, Wu CC, Zhao BY
Toxicon : official journal of the International Society on Toxinology9944-50(2015 Jun)

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
Thaysen-Andersen M, Venkatakrishnan V, Loke I, Laurini C, Diestel S, Parker BL, Packer NH
The Journal of biological chemistry290(14)8789-802(2015 Apr)

Neurocognitive and psychotiform behavioral alterations and enhanced hippocampal long-term potentiation in transgenic mice displaying neuropathological features of human alpha-mannosidosis.
D'Hooge R, L?llmann-Rauch R, Beckers T, Balschun D, Schwake M, Reiss K, von Figura K, Saftig P
The Journal of neuroscience : the official journal of the Society for Neuroscience25(28)6539-49(2005 Jul)

Targeted disruption of the lysosomal alpha-mannosidase gene results in mice resembling a mild form of human alpha-mannosidosis.
Stinchi S, L?llmann-Rauch R, Hartmann D, Coenen R, Beccari T, Orlacchio A, von Figura K, Saftig P
Human molecular genetics8(8)1365-72(1999 Aug)

Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA.
Beccari T, Appolloni MG, Costanzi E, Stinchi S, Stirling JL, Della Fazia MA, Servillo G, Viola MP, Orlacchio A
The Biochemical journal327 ( Pt 1)45-9(1997 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that hepatocyte expression of Man2a1 (Golgi apparatus alpha-mannosidase) and Man2b1 (lysosome alpha-mannosidase) is down-regulated by biological toxin swainsonine as part of locoweed/Oxytropis kansuensis hepatotoxic damage.
Title: The effects of swainsonine on the activity and expression of ?-mannosidase in BRL-3A cells.

The following Man2b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Man2b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05333	NM_199404.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$741.30 $1059.00
ORa59452	XM_017601311.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa59453	XM_017601312.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa59454	XM_017601313.1 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa38718	XM_006255273.3 Latest version!	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Man2b1 ( NM_199404.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955436.1 Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), mRNA.

Price & Availability↑

CloneID	ORa05333
Clone ID Related Accession (Same CDS sequence)	NM_199404.1
Accession Version	NM_199404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-mannosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC061819.1. On Jan 12, 2004 this sequence version replaced XM_341658.1. ##Evidence-Data-START## Transcript exon combination :: BC061819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACA 
GTAGACCAAT ACTACTACGG CATCATGAGT GACGTCCAGC ATGCATCTGT TCAGTACATC 
CTAGACTCAG TGATTTACTC TCTACTGAAC GATCCCACCC GTCGCTTCAT ATACGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAATG TAACACAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTAAT GAATGATGAG 
GCAGCCACTC ACTATGGTGC TATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAG 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ACATTGACCC TTTTGGCCAT 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTCGGGCGC 
ATTGACTACC AAGATAAATT TAACCGGAAG AGAAAGCTGA AGATGGAGGA ACTCTGGAGG 
GCTAGCGCCA GCTTGAAGCC CCCTGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCTC CAAAAGACCT GTGCTGGGAT GTGCTGTGTA CAGACCCTCC CGTGGTGGAC 
GATCCCACAA GCCCTGAGTT TAATGCCAAT AAGTTGGTTG ATTACTTCCT GAACCTCGCC 
TCTTCCCAGA AAAAGTATTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTTATCCG CCTGGTCAAC 
GAGCAGCAGG CAAACGGGAG CAAGGTCCAC GTGCTCTACT CCACCCCTTC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACTTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCTGATGGCC CCCACATGTT CTGGACTGGC TATTTCTCCA GTAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAGGCCA AAGTGGGACC CTACGGCTCA GGAGACAGTG CACCCCTCAA TGAGGCAATG 
GCGGTGCTGC AGCACCACGA TGCTGTCACT GGTACTGCAA GGCAGAATGT TGTCAATGAC 
TATGCGAAGC AGCTGGCGGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCTTTACAA ACAGAACTTC TCATTCTGTC GGGAGATCAA CATCAGTATC 
TGCCCAGCCA GCCAGACGTC AGAGCACTTC CGGGTAATTA TTTACAACCC CGTGGGGCGG 
AAGGTGGATC TGATGGTTCG GCTGCCGGTC AGTGAAGGCA TCTTCCTTGT GAAGGACCCT 
AACGATAGGA GAATTTCCAG TAATGTGGTG ATGGTTCCCA GTGCTTACAG TAAGACGTAT 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAT CTACTCTGTG 
AACAAAATGA GTGGCCATAA CCATCAGGCC CACAACCTAA CGGCCAGGCC CAAGAAATCC 
AAGTCCCGTG TCTTAGTCAT TGAAAATAAG TACATACGGG CCACATTTGA CTCTGACACA 
GGGTTATTGA GGAAGATTGA AAACCTGGAA CAGAACATTT CGCTCCCTGT GAGACAAGGC 
TTCTTTTGGT ACAATGCCAG CGCTGGTGAT GAGGAGAGTC CTCAGGCCTC TGGTGCCTAC 
ATCTTCAGAC CCAGTCATCG TAAACCACTG CCTGTGAGCC ACTGGGCTCA GGTCACTTTG 
GTGAAGACAA ACTTGGTGCA GGAGGTACAC CAGAATTTTT CAGCCTGGTG TTCCCAAGTG 
ATTCGCCTGT ATGAAGGACA GCGTCATTTG GAGCTGGAGT GGACGGTGGG ACCAATCCCA 
GTGAAAGATG ACTGGGGAAA GGAAGTCATC AGCCGCTTTA ACACCCCGAT GAGAACCAGG 
GGGCAGTTCT TCACGGACAG CAACGGCCGG GAAATCCTGA AGAGGAGGGA CGATTTCCGA 
CCCACCTGGA CATTAAATCA GACTGAACCA GTGGCTGGAA ATTACTACCC TGTCAACACT 
CGCATTTACA TCACGGACGG GCATATGCAG CTGACCGTGC TGACCGACCG TTCCCAGGGG 
GGCAGCAGTC TGCTGGATGG CTCATTGGAG CTCATGGTTC ACCGACGGCT GTTGGTAGAT 
GATGAACGTG GGGTGGCGGA ACCCTTGCTG GAGACAGATA CGGGGGATAA GGTTCGAGGG 
CGCCACCTTG TGATTTTGAG TTCTGTCAGT GATGCGGCTG CAAGACACCG ACTGCTGGCG 
GAGCAGGAAG TCCTGGCCCC TCAGGTGGTG CTGGCCCACG GGGGTAGCAG TCCCTATCAC 
TCGCAGGCAC CAAAAATGCA GTTCTCCGCA CTTCGCCGGG AGCTACCTCC GCAGGTGCAT 
CTGCTCACCC TGGCTCGCTG GGGTCCAAAG ATGCTGCTGC TGCGCTTGGA GCACCAGTTC 
GCTGTGAAGG AAGATTCAAA TCGCAACCTG AGCTCTCCGG TGACCTTGAA CTTGCAGAAC 
CTGTTCAAGA CCTTCACTAT CAACTACCTG CAGGAGACCA CACTGGCAGC CAACCAGCCC 
CTATCCAGGG TTTCCAGGCT CAAGTGGATG ACAGATACTG GTCCCATCTC CTACCCTGCT 
CCTTCCCGGT TGGACCCTAC CTCAATCACA CTGCAGCCTA TGCAAATCCG TACCTTCCTG 
GCCTCGGTTC AATGGCAGAG ACTCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955436.1
CDS	26..3055
Translation	MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGIMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTGPISYPAPSRLDPTSITLQPMQIRTFLASVQWQRLI

Target ORF information:

RefSeq Version	NM_199404.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199404.1

ORF Insert Sequence:

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACA 
GTAGACCAAT ACTACTACGG CATCATGAGT GACGTCCAGC ATGCATCTGT TCAGTACATC 
CTAGACTCAG TGATTTACTC TCTACTGAAC GATCCCACCC GTCGCTTCAT ATACGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAATG TAACACAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTAAT GAATGATGAG 
GCAGCCACTC ACTATGGTGC TATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAG 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ACATTGACCC TTTTGGCCAT 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTCGGGCGC 
ATTGACTACC AAGATAAATT TAACCGGAAG AGAAAGCTGA AGATGGAGGA ACTCTGGAGG 
GCTAGCGCCA GCTTGAAGCC CCCTGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCTC CAAAAGACCT GTGCTGGGAT GTGCTGTGTA CAGACCCTCC CGTGGTGGAC 
GATCCCACAA GCCCTGAGTT TAATGCCAAT AAGTTGGTTG ATTACTTCCT GAACCTCGCC 
TCTTCCCAGA AAAAGTATTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTTATCCG CCTGGTCAAC 
GAGCAGCAGG CAAACGGGAG CAAGGTCCAC GTGCTCTACT CCACCCCTTC GTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA TCTCACTTGG ACAGTGAAGG AGGACGACTT CTTTCCTTAT 
GCTGATGGCC CCCACATGTT CTGGACTGGC TATTTCTCCA GTAGGCCAGC CCTCAAGCGC 
TATGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAAGC ACTGGTGGGT 
CCCGAGGCCA AAGTGGGACC CTACGGCTCA GGAGACAGTG CACCCCTCAA TGAGGCAATG 
GCGGTGCTGC AGCACCACGA TGCTGTCACT GGTACTGCAA GGCAGAATGT TGTCAATGAC 
TATGCGAAGC AGCTGGCGGC AGGCTGGGGA CCCTGTGAGG TTCTCGTGAG CAACGCTCTG 
GCGCGACTCA GCCTTTACAA ACAGAACTTC TCATTCTGTC GGGAGATCAA CATCAGTATC 
TGCCCAGCCA GCCAGACGTC AGAGCACTTC CGGGTAATTA TTTACAACCC CGTGGGGCGG 
AAGGTGGATC TGATGGTTCG GCTGCCGGTC AGTGAAGGCA TCTTCCTTGT GAAGGACCCT 
AACGATAGGA GAATTTCCAG TAATGTGGTG ATGGTTCCCA GTGCTTACAG TAAGACGTAT 
CAATGGGAGC TGCTGTTCCC AGCCTCAGTG CCTGCCCTGG GCTTCAGCAT CTACTCTGTG 
AACAAAATGA GTGGCCATAA CCATCAGGCC CACAACCTAA CGGCCAGGCC CAAGAAATCC 
AAGTCCCGTG TCTTAGTCAT TGAAAATAAG TACATACGGG CCACATTTGA CTCTGACACA 
GGGTTATTGA GGAAGATTGA AAACCTGGAA CAGAACATTT CGCTCCCTGT GAGACAAGGC 
TTCTTTTGGT ACAATGCCAG CGCTGGTGAT GAGGAGAGTC CTCAGGCCTC TGGTGCCTAC 
ATCTTCAGAC CCAGTCATCG TAAACCACTG CCTGTGAGCC ACTGGGCTCA GGTCACTTTG 
GTGAAGACAA ACTTGGTGCA GGAGGTACAC CAGAATTTTT CAGCCTGGTG TTCCCAAGTG 
ATTCGCCTGT ATGAAGGACA GCGTCATTTG GAGCTGGAGT GGACGGTGGG ACCAATCCCA 
GTGAAAGATG ACTGGGGAAA GGAAGTCATC AGCCGCTTTA ACACCCCGAT GAGAACCAGG 
GGGCAGTTCT TCACGGACAG CAACGGCCGG GAAATCCTGA AGAGGAGGGA CGATTTCCGA 
CCCACCTGGA CATTAAATCA GACTGAACCA GTGGCTGGAA ATTACTACCC TGTCAACACT 
CGCATTTACA TCACGGACGG GCATATGCAG CTGACCGTGC TGACCGACCG TTCCCAGGGG 
GGCAGCAGTC TGCTGGATGG CTCATTGGAG CTCATGGTTC ACCGACGGCT GTTGGTAGAT 
GATGAACGTG GGGTGGCGGA ACCCTTGCTG GAGACAGATA CGGGGGATAA GGTTCGAGGG 
CGCCACCTTG TGATTTTGAG TTCTGTCAGT GATGCGGCTG CAAGACACCG ACTGCTGGCG 
GAGCAGGAAG TCCTGGCCCC TCAGGTGGTG CTGGCCCACG GGGGTAGCAG TCCCTATCAC 
TCGCAGGCAC CAAAAATGCA GTTCTCCGCA CTTCGCCGGG AGCTACCTCC GCAGGTGCAT 
CTGCTCACCC TGGCTCGCTG GGGTCCAAAG ATGCTGCTGC TGCGCTTGGA GCACCAGTTC 
GCTGTGAAGG AAGATTCAAA TCGCAACCTG AGCTCTCCGG TGACCTTGAA CTTGCAGAAC 
CTGTTCAAGA CCTTCACTAT CAACTACCTG CAGGAGACCA CACTGGCAGC CAACCAGCCC 
CTATCCAGGG TTTCCAGGCT CAAGTGGATG ACAGATACTG GTCCCATCTC CTACCCTGCT 
CCTTCCCGGT TGGACCCTAC CTCAATCACA CTGCAGCCTA TGCAAATCCG TACCTTCCTG 
GCCTCGGTTC AATGGCAGAG ACTCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_017601311.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456800.1 Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59452
Clone ID Related Accession (Same CDS sequence)	XM_017601311.1
Accession Version	XM_017601311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGCAGGCAAA CGGGAGCAAG 
GTCCACGTGC TCTACTCCAC CCCTTCGTGT TACCTCTGGG AGCTGAACAA GGCCAATCTC 
ACTTGGACAG TGAAGGAGGA CGACTTCTTT CCTTATGCTG ATGGCCCCCA CATGTTCTGG 
ACTGGCTATT TCTCCAGTAG GCCAGCCCTC AAGCGCTATG AGCGCCTCAG CTACAACTTC 
CTGCAGGTGT GCAACCAGCT GGAAGCACTG GTGGGTCCCG AGGCCAAAGT GGGACCCTAC 
GGCTCAGGAG ACAGTGCACC CCTCAATGAG GCAATGGCGG TGCTGCAGCA CCACGATGCT 
GTCACTGGTA CTGCAAGACA GAATGTTGTC AATGACTATG CGAAGCAGCT GGCGGCAGGC 
TGGGGACCCT GTGAGGTTCT CGTGAGCAAC GCTCTGGCGC GACTCAGCCT TTACAAACAG 
AACTTCTCAT TCTGTCGGGA GATCAACATC AGTATCTGCC CAGCCAGCCA GACGTCAGAG 
CACTTCCGGG TAATTATTTA CAACCCCGTG GGGCGGAAGG TGGATCTGAT GGTTCGGCTG 
CCGGTCAGTG AAGGCATCTT CCTTGTGAAG GACCCTAACG ATAGGAGAAT TTCCAGTAAT 
GTGGTGATGG TTCCCAGTGC TTACAGTAAG ACGTATCAAT GGGAGCTGCT GTTCCCAGCC 
TCAGTGCCTG CCCTGGGCTT CAGCATCTAC TCTGTGAACA AAATGAGTGG CCATAACCAT 
CAGGCCCACA ACCTAACGGC CAGGCCCAAG AAATCCAAGT CCCGTGTCTT AGTCATTGAA 
AATAAGTACA TACGGGCCAC ATTTGACTCT GACACAGGGT TATTGAGGAA GATTGAAAAC 
CTGGAACAGA ACATTTCGCT CCCTGTGAGA CAAGGCTTCT TTTGGTACAA TGCCAGCGCT 
GGTGATGAGG AGAGTCCTCA GGCCTCTGGT GCCTACATCT TCAGACCCAG TCATCGTAAA 
CCACTGCCTG TGAGCCACTG GGCTCAGGTC ACTTTGGTGA AGACAAACTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTATGA AGGACAGCGT 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA AAGATGACTG GGGAAAGGAA 
GTCATCAGCC GCTTTAACAC CCCGATGAGA ACCAGGGGGC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGACGAT TTCCGACCCA CCTGGACATT AAATCAGACT 
GAACCAGTGG CTGGAAATTA CTACCCTGTC AACACTCGCA TTTACATCAC GGACGGGCAT 
ATGCAGCTGA CCGTGCTGAC CGACCGTTCC CAGGGGGGCA GCAGTCTGCT GGATGGCTCA 
TTGGAGCTCA TGGTTCACCG ACGGCTGTTG GTAGATGATG AACGTGGGGT GGCGGAACCC 
TTGCTGGAGA CAGATACGGG GGATAAGGTT CGAGGGCGCC ACCTTGTGAT TTTGAGTTCT 
GTCAGTGATG CGGCTGCAAG ACACCGACTG CTGGCGGAGC AGGAAGTCCT GGCCCCTCAG 
GTGGTGCTGG CCCACGGGGG TAGCAGTCCC TATCACTCGC AGGCACCAAA AATGCAGTTC 
TCCGCACTTC GCCGGGAGCT ACCTCCGCAG GTGCATCTGC TCACCCTGGC TCGCTGGGGT 
CCAAAGATGC TGCTGCTGCG CTTGGAGCAC CAGTTCGCTG TGAAGGAAGA TTCAAATCGC 
AACCTGAGCT CTCCGGTGAC CTTGAACTTG CAGAACCTGT TCAAGACCTT CACTATCAAC 
TACCTGCAGG AGACCACACT GGCAGCCAAC CAGCCCCTAT CCAGGGTTTC CAGGCTCAAG 
TGGATGACAG ATACTGATAT CGCGTCTGGG AAGATGGCTC TGATTAAGAA TGGTGTTCTT 
CCAGAGTGTT CGAGATTAGC TTCCAGCACT GCATTAGGTG GTTCACCAGC TGTAACGGCA 
GCCCTCGAGG GATCCTACGC CAGTGGCCTT TATGGTCCGT GGTCACCTTC ACTTTCATGC 
ACACACCCAC ACATGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456800.1
CDS	66..3206
Translation	MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGMDPHLLTFMPIVMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTDIASGKMALIKNGVLPECSRLASSTALGGSPAVTAALEGSYASGLYGPWSPSLSCTHPHMK

Target ORF information:

RefSeq Version	XM_017601311.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601311.1

ORF Insert Sequence:

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGCAGGCAAA CGGGAGCAAG 
GTCCACGTGC TCTACTCCAC CCCTTCGTGT TACCTCTGGG AGCTGAACAA GGCCAATCTC 
ACTTGGACAG TGAAGGAGGA CGACTTCTTT CCTTATGCTG ATGGCCCCCA CATGTTCTGG 
ACTGGCTATT TCTCCAGTAG GCCAGCCCTC AAGCGCTATG AGCGCCTCAG CTACAACTTC 
CTGCAGGTGT GCAACCAGCT GGAAGCACTG GTGGGTCCCG AGGCCAAAGT GGGACCCTAC 
GGCTCAGGAG ACAGTGCACC CCTCAATGAG GCAATGGCGG TGCTGCAGCA CCACGATGCT 
GTCACTGGTA CTGCAAGACA GAATGTTGTC AATGACTATG CGAAGCAGCT GGCGGCAGGC 
TGGGGACCCT GTGAGGTTCT CGTGAGCAAC GCTCTGGCGC GACTCAGCCT TTACAAACAG 
AACTTCTCAT TCTGTCGGGA GATCAACATC AGTATCTGCC CAGCCAGCCA GACGTCAGAG 
CACTTCCGGG TAATTATTTA CAACCCCGTG GGGCGGAAGG TGGATCTGAT GGTTCGGCTG 
CCGGTCAGTG AAGGCATCTT CCTTGTGAAG GACCCTAACG ATAGGAGAAT TTCCAGTAAT 
GTGGTGATGG TTCCCAGTGC TTACAGTAAG ACGTATCAAT GGGAGCTGCT GTTCCCAGCC 
TCAGTGCCTG CCCTGGGCTT CAGCATCTAC TCTGTGAACA AAATGAGTGG CCATAACCAT 
CAGGCCCACA ACCTAACGGC CAGGCCCAAG AAATCCAAGT CCCGTGTCTT AGTCATTGAA 
AATAAGTACA TACGGGCCAC ATTTGACTCT GACACAGGGT TATTGAGGAA GATTGAAAAC 
CTGGAACAGA ACATTTCGCT CCCTGTGAGA CAAGGCTTCT TTTGGTACAA TGCCAGCGCT 
GGTGATGAGG AGAGTCCTCA GGCCTCTGGT GCCTACATCT TCAGACCCAG TCATCGTAAA 
CCACTGCCTG TGAGCCACTG GGCTCAGGTC ACTTTGGTGA AGACAAACTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTATGA AGGACAGCGT 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA AAGATGACTG GGGAAAGGAA 
GTCATCAGCC GCTTTAACAC CCCGATGAGA ACCAGGGGGC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGACGAT TTCCGACCCA CCTGGACATT AAATCAGACT 
GAACCAGTGG CTGGAAATTA CTACCCTGTC AACACTCGCA TTTACATCAC GGACGGGCAT 
ATGCAGCTGA CCGTGCTGAC CGACCGTTCC CAGGGGGGCA GCAGTCTGCT GGATGGCTCA 
TTGGAGCTCA TGGTTCACCG ACGGCTGTTG GTAGATGATG AACGTGGGGT GGCGGAACCC 
TTGCTGGAGA CAGATACGGG GGATAAGGTT CGAGGGCGCC ACCTTGTGAT TTTGAGTTCT 
GTCAGTGATG CGGCTGCAAG ACACCGACTG CTGGCGGAGC AGGAAGTCCT GGCCCCTCAG 
GTGGTGCTGG CCCACGGGGG TAGCAGTCCC TATCACTCGC AGGCACCAAA AATGCAGTTC 
TCCGCACTTC GCCGGGAGCT ACCTCCGCAG GTGCATCTGC TCACCCTGGC TCGCTGGGGT 
CCAAAGATGC TGCTGCTGCG CTTGGAGCAC CAGTTCGCTG TGAAGGAAGA TTCAAATCGC 
AACCTGAGCT CTCCGGTGAC CTTGAACTTG CAGAACCTGT TCAAGACCTT CACTATCAAC 
TACCTGCAGG AGACCACACT GGCAGCCAAC CAGCCCCTAT CCAGGGTTTC CAGGCTCAAG 
TGGATGACAG ATACTGATAT CGCGTCTGGG AAGATGGCTC TGATTAAGAA TGGTGTTCTT 
CCAGAGTGTT CGAGATTAGC TTCCAGCACT GCATTAGGTG GTTCACCAGC TGTAACGGCA 
GCCCTCGAGG GATCCTACGC CAGTGGCCTT TATGGTCCGT GGTCACCTTC ACTTTCATGC 
ACACACCCAC ACATGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_017601312.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456801.1 Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59453
Clone ID Related Accession (Same CDS sequence)	XM_017601312.1
Accession Version	XM_017601312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGGCAAACGG GAGCAAGGTC 
CACGTGCTCT ACTCCACCCC TTCGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACT 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCTGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTCT CCAGTAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGCTA CAACTTCCTG 
CAGGTGTGCA ACCAGCTGGA AGCACTGGTG GGTCCCGAGG CCAAAGTGGG ACCCTACGGC 
TCAGGAGACA GTGCACCCCT CAATGAGGCA ATGGCGGTGC TGCAGCACCA CGATGCTGTC 
ACTGGTACTG CAAGACAGAA TGTTGTCAAT GACTATGCGA AGCAGCTGGC GGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCTTTA CAAACAGAAC 
TTCTCATTCT GTCGGGAGAT CAACATCAGT ATCTGCCCAG CCAGCCAGAC GTCAGAGCAC 
TTCCGGGTAA TTATTTACAA CCCCGTGGGG CGGAAGGTGG ATCTGATGGT TCGGCTGCCG 
GTCAGTGAAG GCATCTTCCT TGTGAAGGAC CCTAACGATA GGAGAATTTC CAGTAATGTG 
GTGATGGTTC CCAGTGCTTA CAGTAAGACG TATCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CATCTACTCT GTGAACAAAA TGAGTGGCCA TAACCATCAG 
GCCCACAACC TAACGGCCAG GCCCAAGAAA TCCAAGTCCC GTGTCTTAGT CATTGAAAAT 
AAGTACATAC GGGCCACATT TGACTCTGAC ACAGGGTTAT TGAGGAAGAT TGAAAACCTG 
GAACAGAACA TTTCGCTCCC TGTGAGACAA GGCTTCTTTT GGTACAATGC CAGCGCTGGT 
GATGAGGAGA GTCCTCAGGC CTCTGGTGCC TACATCTTCA GACCCAGTCA TCGTAAACCA 
CTGCCTGTGA GCCACTGGGC TCAGGTCACT TTGGTGAAGA CAAACTTGGT GCAGGAGGTA 
CACCAGAATT TTTCAGCCTG GTGTTCCCAA GTGATTCGCC TGTATGAAGG ACAGCGTCAT 
TTGGAGCTGG AGTGGACGGT GGGACCAATC CCAGTGAAAG ATGACTGGGG AAAGGAAGTC 
ATCAGCCGCT TTAACACCCC GATGAGAACC AGGGGGCAGT TCTTCACGGA CAGCAACGGC 
CGGGAAATCC TGAAGAGGAG GGACGATTTC CGACCCACCT GGACATTAAA TCAGACTGAA 
CCAGTGGCTG GAAATTACTA CCCTGTCAAC ACTCGCATTT ACATCACGGA CGGGCATATG 
CAGCTGACCG TGCTGACCGA CCGTTCCCAG GGGGGCAGCA GTCTGCTGGA TGGCTCATTG 
GAGCTCATGG TTCACCGACG GCTGTTGGTA GATGATGAAC GTGGGGTGGC GGAACCCTTG 
CTGGAGACAG ATACGGGGGA TAAGGTTCGA GGGCGCCACC TTGTGATTTT GAGTTCTGTC 
AGTGATGCGG CTGCAAGACA CCGACTGCTG GCGGAGCAGG AAGTCCTGGC CCCTCAGGTG 
GTGCTGGCCC ACGGGGGTAG CAGTCCCTAT CACTCGCAGG CACCAAAAAT GCAGTTCTCC 
GCACTTCGCC GGGAGCTACC TCCGCAGGTG CATCTGCTCA CCCTGGCTCG CTGGGGTCCA 
AAGATGCTGC TGCTGCGCTT GGAGCACCAG TTCGCTGTGA AGGAAGATTC AAATCGCAAC 
CTGAGCTCTC CGGTGACCTT GAACTTGCAG AACCTGTTCA AGACCTTCAC TATCAACTAC 
CTGCAGGAGA CCACACTGGC AGCCAACCAG CCCCTATCCA GGGTTTCCAG GCTCAAGTGG 
ATGACAGATA CTGATATCGC GTCTGGGAAG ATGGCTCTGA TTAAGAATGG TGTTCTTCCA 
GAGTGTTCGA GATTAGCTTC CAGCACTGCA TTAGGTGGTT CACCAGCTGT AACGGCAGCC 
CTCGAGGGAT CCTACGCCAG TGGCCTTTAT GGTCCGTGGT CACCTTCACT TTCATGCACA 
CACCCACACA TGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456801.1
CDS	66..3203
Translation	MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGMDPHLLTFMPIVMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTDIASGKMALIKNGVLPECSRLASSTALGGSPAVTAALEGSYASGLYGPWSPSLSCTHPHMK

Target ORF information:

RefSeq Version	XM_017601312.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601312.1

ORF Insert Sequence:

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGGCAAACGG GAGCAAGGTC 
CACGTGCTCT ACTCCACCCC TTCGTGTTAC CTCTGGGAGC TGAACAAGGC CAATCTCACT 
TGGACAGTGA AGGAGGACGA CTTCTTTCCT TATGCTGATG GCCCCCACAT GTTCTGGACT 
GGCTATTTCT CCAGTAGGCC AGCCCTCAAG CGCTATGAGC GCCTCAGCTA CAACTTCCTG 
CAGGTGTGCA ACCAGCTGGA AGCACTGGTG GGTCCCGAGG CCAAAGTGGG ACCCTACGGC 
TCAGGAGACA GTGCACCCCT CAATGAGGCA ATGGCGGTGC TGCAGCACCA CGATGCTGTC 
ACTGGTACTG CAAGACAGAA TGTTGTCAAT GACTATGCGA AGCAGCTGGC GGCAGGCTGG 
GGACCCTGTG AGGTTCTCGT GAGCAACGCT CTGGCGCGAC TCAGCCTTTA CAAACAGAAC 
TTCTCATTCT GTCGGGAGAT CAACATCAGT ATCTGCCCAG CCAGCCAGAC GTCAGAGCAC 
TTCCGGGTAA TTATTTACAA CCCCGTGGGG CGGAAGGTGG ATCTGATGGT TCGGCTGCCG 
GTCAGTGAAG GCATCTTCCT TGTGAAGGAC CCTAACGATA GGAGAATTTC CAGTAATGTG 
GTGATGGTTC CCAGTGCTTA CAGTAAGACG TATCAATGGG AGCTGCTGTT CCCAGCCTCA 
GTGCCTGCCC TGGGCTTCAG CATCTACTCT GTGAACAAAA TGAGTGGCCA TAACCATCAG 
GCCCACAACC TAACGGCCAG GCCCAAGAAA TCCAAGTCCC GTGTCTTAGT CATTGAAAAT 
AAGTACATAC GGGCCACATT TGACTCTGAC ACAGGGTTAT TGAGGAAGAT TGAAAACCTG 
GAACAGAACA TTTCGCTCCC TGTGAGACAA GGCTTCTTTT GGTACAATGC CAGCGCTGGT 
GATGAGGAGA GTCCTCAGGC CTCTGGTGCC TACATCTTCA GACCCAGTCA TCGTAAACCA 
CTGCCTGTGA GCCACTGGGC TCAGGTCACT TTGGTGAAGA CAAACTTGGT GCAGGAGGTA 
CACCAGAATT TTTCAGCCTG GTGTTCCCAA GTGATTCGCC TGTATGAAGG ACAGCGTCAT 
TTGGAGCTGG AGTGGACGGT GGGACCAATC CCAGTGAAAG ATGACTGGGG AAAGGAAGTC 
ATCAGCCGCT TTAACACCCC GATGAGAACC AGGGGGCAGT TCTTCACGGA CAGCAACGGC 
CGGGAAATCC TGAAGAGGAG GGACGATTTC CGACCCACCT GGACATTAAA TCAGACTGAA 
CCAGTGGCTG GAAATTACTA CCCTGTCAAC ACTCGCATTT ACATCACGGA CGGGCATATG 
CAGCTGACCG TGCTGACCGA CCGTTCCCAG GGGGGCAGCA GTCTGCTGGA TGGCTCATTG 
GAGCTCATGG TTCACCGACG GCTGTTGGTA GATGATGAAC GTGGGGTGGC GGAACCCTTG 
CTGGAGACAG ATACGGGGGA TAAGGTTCGA GGGCGCCACC TTGTGATTTT GAGTTCTGTC 
AGTGATGCGG CTGCAAGACA CCGACTGCTG GCGGAGCAGG AAGTCCTGGC CCCTCAGGTG 
GTGCTGGCCC ACGGGGGTAG CAGTCCCTAT CACTCGCAGG CACCAAAAAT GCAGTTCTCC 
GCACTTCGCC GGGAGCTACC TCCGCAGGTG CATCTGCTCA CCCTGGCTCG CTGGGGTCCA 
AAGATGCTGC TGCTGCGCTT GGAGCACCAG TTCGCTGTGA AGGAAGATTC AAATCGCAAC 
CTGAGCTCTC CGGTGACCTT GAACTTGCAG AACCTGTTCA AGACCTTCAC TATCAACTAC 
CTGCAGGAGA CCACACTGGC AGCCAACCAG CCCCTATCCA GGGTTTCCAG GCTCAAGTGG 
ATGACAGATA CTGATATCGC GTCTGGGAAG ATGGCTCTGA TTAAGAATGG TGTTCTTCCA 
GAGTGTTCGA GATTAGCTTC CAGCACTGCA TTAGGTGGTT CACCAGCTGT AACGGCAGCC 
CTCGAGGGAT CCTACGCCAG TGGCCTTTAT GGTCCGTGGT CACCTTCACT TTCATGCACA 
CACCCACACA TGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_017601313.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456802.1 Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa59454
Clone ID Related Accession (Same CDS sequence)	XM_017601313.1
Accession Version	XM_017601313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-mannosidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATCATGAGT GACGTCCAGC ATGCATCTGT TCAGTACATC 
CTAGACTCAG TGATTTACTC TCTACTGAAC GATCCCACCC GTCGCTTCAT ATACGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAATG TAACACAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTAAT GAATGATGAG 
GCAGCCACTC ACTATGGTGC TATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAG 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ACATTGACCC TTTTGGCCAT 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTCGGGCGC 
ATTGACTACC AAGATAAATT TAACCGGAAG AGAAAGCTGA AGATGGAGGA ACTCTGGAGG 
GCTAGCGCCA GCTTGAAGCC CCCTGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCTC CAAAAGACCT GTGCTGGGAT GTGCTGTGTA CAGACCCTCC CGTGGTGGAC 
GATCCCACAA GCCCTGAGTT TAATGCCAAT AAGTTGGTTG ATTACTTCCT GAACCTCGCC 
TCTTCCCAGA AAAAGTATTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTTATCCG CCTGGTCAAC 
GAGCAGCAGC AGGCAAACGG GAGCAAGGTC CACGTGCTCT ACTCCACCCC TTCGTGTTAC 
CTCTGGGAGC TGAACAAGGC CAATCTCACT TGGACAGTGA AGGAGGACGA CTTCTTTCCT 
TATGCTGATG GCCCCCACAT GTTCTGGACT GGCTATTTCT CCAGTAGGCC AGCCCTCAAG 
CGCTATGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA AGCACTGGTG 
GGTCCCGAGG CCAAAGTGGG ACCCTACGGC TCAGGAGACA GTGCACCCCT CAATGAGGCA 
ATGGCGGTGC TGCAGCACCA CGATGCTGTC ACTGGTACTG CAAGACAGAA TGTTGTCAAT 
GACTATGCGA AGCAGCTGGC GGCAGGCTGG GGACCCTGTG AGGTTCTCGT GAGCAACGCT 
CTGGCGCGAC TCAGCCTTTA CAAACAGAAC TTCTCATTCT GTCGGGAGAT CAACATCAGT 
ATCTGCCCAG CCAGCCAGAC GTCAGAGCAC TTCCGGGTAA TTATTTACAA CCCCGTGGGG 
CGGAAGGTGG ATCTGATGGT TCGGCTGCCG GTCAGTGAAG GCATCTTCCT TGTGAAGGAC 
CCTAACGATA GGAGAATTTC CAGTAATGTG GTGATGGTTC CCAGTGCTTA CAGTAAGACG 
TATCAATGGG AGCTGCTGTT CCCAGCCTCA GTGCCTGCCC TGGGCTTCAG CATCTACTCT 
GTGAACAAAA TGAGTGGCCA TAACCATCAG GCCCACAACC TAACGGCCAG GCCCAAGAAA 
TCCAAGTCCC GTGTCTTAGT CATTGAAAAT AAGTACATAC GGGCCACATT TGACTCTGAC 
ACAGGGTTAT TGAGGAAGAT TGAAAACCTG GAACAGAACA TTTCGCTCCC TGTGAGACAA 
GGCTTCTTTT GGTACAATGC CAGCGCTGGT GATGAGGAGA GTCCTCAGGC CTCTGGTGCC 
TACATCTTCA GACCCAGTCA TCGTAAACCA CTGCCTGTGA GCCACTGGGC TCAGGTCACT 
TTGGTGAAGA CAAACTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTATGAAGG ACAGCGTCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAAAG ATGACTGGGG AAAGGAAGTC ATCAGCCGCT TTAACACCCC GATGAGAACC 
AGGGGGCAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGACGATTTC 
CGACCCACCT GGACATTAAA TCAGACTGAA CCAGTGGCTG GAAATTACTA CCCTGTCAAC 
ACTCGCATTT ACATCACGGA CGGGCATATG CAGCTGACCG TGCTGACCGA CCGTTCCCAG 
GGGGGCAGCA GTCTGCTGGA TGGCTCATTG GAGCTCATGG TTCACCGACG GCTGTTGGTA 
GATGATGAAC GTGGGGTGGC GGAACCCTTG CTGGAGACAG ATACGGGGGA TAAGGTTCGA 
GGGCGCCACC TTGTGATTTT GAGTTCTGTC AGTGATGCGG CTGCAAGACA CCGACTGCTG 
GCGGAGCAGG AAGTCCTGGC CCCTCAGGTG GTGCTGGCCC ACGGGGGTAG CAGTCCCTAT 
CACTCGCAGG CACCAAAAAT GCAGTTCTCC GCACTTCGCC GGGAGCTACC TCCGCAGGTG 
CATCTGCTCA CCCTGGCTCG CTGGGGTCCA AAGATGCTGC TGCTGCGCTT GGAGCACCAG 
TTCGCTGTGA AGGAAGATTC AAATCGCAAC CTGAGCTCTC CGGTGACCTT GAACTTGCAG 
AACCTGTTCA AGACCTTCAC TATCAACTAC CTGCAGGAGA CCACACTGGC AGCCAACCAG 
CCCCTATCCA GGGTTTCCAG GCTCAAGTGG ATGACAGATA CTGATATCGC GTCTGGGAAG 
ATGGCTCTGA TTAAGAATGG TGTTCTTCCA GAGTGTTCGA GATTAGCTTC CAGCACTGCA 
TTAGGTGGTT CACCAGCTGT AACGGCAGCC CTCGAGGGAT CCTACGCCAG TGGCCTTTAT 
GGTCCGTGGT CACCTTCACT TTCATGCACA CACCCACACA TGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456802.1
CDS	66..3173
Translation	MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGIMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTDIASGKMALIKNGVLPECSRLASSTALGGSPAVTAALEGSYASGLYGPWSPSLSCTHPHMK

Target ORF information:

RefSeq Version	XM_017601313.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601313.1

ORF Insert Sequence:

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATCATGAGT GACGTCCAGC ATGCATCTGT TCAGTACATC 
CTAGACTCAG TGATTTACTC TCTACTGAAC GATCCCACCC GTCGCTTCAT ATACGTGGAA 
ATGGCTTTCT TCTCCCGTTG GTGGAAGCAG CAGACCAATG TAACACAGGA CGCTGTACGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GTCAATGGTG GCTGGGTAAT GAATGATGAG 
GCAGCCACTC ACTATGGTGC TATTGTGGAC CAGATGACAC TTGGGCTACG CTTCCTGCAG 
GACACATTTG GTAGTGATGG ACTTCCCCGT GTGGCCTGGC ACATTGACCC TTTTGGCCAT 
TCTCGAGAAC AGGCCTCCCT GTTTGCCCAG ATGGGTTTTG ATGGCTTCTT CCTCGGGCGC 
ATTGACTACC AAGATAAATT TAACCGGAAG AGAAAGCTGA AGATGGAGGA ACTCTGGAGG 
GCTAGCGCCA GCTTGAAGCC CCCTGCTGCT GACCTCTTTA CTGGTGTGCT CCCCAATAAT 
TACAACCCTC CAAAAGACCT GTGCTGGGAT GTGCTGTGTA CAGACCCTCC CGTGGTGGAC 
GATCCCACAA GCCCTGAGTT TAATGCCAAT AAGTTGGTTG ATTACTTCCT GAACCTCGCC 
TCTTCCCAGA AAAAGTATTA CCGAACCAAC CACACCGTGA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTCAAA AACATGGACA AGCTTATCCG CCTGGTCAAC 
GAGCAGCAGC AGGCAAACGG GAGCAAGGTC CACGTGCTCT ACTCCACCCC TTCGTGTTAC 
CTCTGGGAGC TGAACAAGGC CAATCTCACT TGGACAGTGA AGGAGGACGA CTTCTTTCCT 
TATGCTGATG GCCCCCACAT GTTCTGGACT GGCTATTTCT CCAGTAGGCC AGCCCTCAAG 
CGCTATGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA AGCACTGGTG 
GGTCCCGAGG CCAAAGTGGG ACCCTACGGC TCAGGAGACA GTGCACCCCT CAATGAGGCA 
ATGGCGGTGC TGCAGCACCA CGATGCTGTC ACTGGTACTG CAAGACAGAA TGTTGTCAAT 
GACTATGCGA AGCAGCTGGC GGCAGGCTGG GGACCCTGTG AGGTTCTCGT GAGCAACGCT 
CTGGCGCGAC TCAGCCTTTA CAAACAGAAC TTCTCATTCT GTCGGGAGAT CAACATCAGT 
ATCTGCCCAG CCAGCCAGAC GTCAGAGCAC TTCCGGGTAA TTATTTACAA CCCCGTGGGG 
CGGAAGGTGG ATCTGATGGT TCGGCTGCCG GTCAGTGAAG GCATCTTCCT TGTGAAGGAC 
CCTAACGATA GGAGAATTTC CAGTAATGTG GTGATGGTTC CCAGTGCTTA CAGTAAGACG 
TATCAATGGG AGCTGCTGTT CCCAGCCTCA GTGCCTGCCC TGGGCTTCAG CATCTACTCT 
GTGAACAAAA TGAGTGGCCA TAACCATCAG GCCCACAACC TAACGGCCAG GCCCAAGAAA 
TCCAAGTCCC GTGTCTTAGT CATTGAAAAT AAGTACATAC GGGCCACATT TGACTCTGAC 
ACAGGGTTAT TGAGGAAGAT TGAAAACCTG GAACAGAACA TTTCGCTCCC TGTGAGACAA 
GGCTTCTTTT GGTACAATGC CAGCGCTGGT GATGAGGAGA GTCCTCAGGC CTCTGGTGCC 
TACATCTTCA GACCCAGTCA TCGTAAACCA CTGCCTGTGA GCCACTGGGC TCAGGTCACT 
TTGGTGAAGA CAAACTTGGT GCAGGAGGTA CACCAGAATT TTTCAGCCTG GTGTTCCCAA 
GTGATTCGCC TGTATGAAGG ACAGCGTCAT TTGGAGCTGG AGTGGACGGT GGGACCAATC 
CCAGTGAAAG ATGACTGGGG AAAGGAAGTC ATCAGCCGCT TTAACACCCC GATGAGAACC 
AGGGGGCAGT TCTTCACGGA CAGCAACGGC CGGGAAATCC TGAAGAGGAG GGACGATTTC 
CGACCCACCT GGACATTAAA TCAGACTGAA CCAGTGGCTG GAAATTACTA CCCTGTCAAC 
ACTCGCATTT ACATCACGGA CGGGCATATG CAGCTGACCG TGCTGACCGA CCGTTCCCAG 
GGGGGCAGCA GTCTGCTGGA TGGCTCATTG GAGCTCATGG TTCACCGACG GCTGTTGGTA 
GATGATGAAC GTGGGGTGGC GGAACCCTTG CTGGAGACAG ATACGGGGGA TAAGGTTCGA 
GGGCGCCACC TTGTGATTTT GAGTTCTGTC AGTGATGCGG CTGCAAGACA CCGACTGCTG 
GCGGAGCAGG AAGTCCTGGC CCCTCAGGTG GTGCTGGCCC ACGGGGGTAG CAGTCCCTAT 
CACTCGCAGG CACCAAAAAT GCAGTTCTCC GCACTTCGCC GGGAGCTACC TCCGCAGGTG 
CATCTGCTCA CCCTGGCTCG CTGGGGTCCA AAGATGCTGC TGCTGCGCTT GGAGCACCAG 
TTCGCTGTGA AGGAAGATTC AAATCGCAAC CTGAGCTCTC CGGTGACCTT GAACTTGCAG 
AACCTGTTCA AGACCTTCAC TATCAACTAC CTGCAGGAGA CCACACTGGC AGCCAACCAG 
CCCCTATCCA GGGTTTCCAG GCTCAAGTGG ATGACAGATA CTGATATCGC GTCTGGGAAG 
ATGGCTCTGA TTAAGAATGG TGTTCTTCCA GAGTGTTCGA GATTAGCTTC CAGCACTGCA 
TTAGGTGGTT CACCAGCTGT AACGGCAGCC CTCGAGGGAT CCTACGCCAG TGGCCTTTAT 
GGTCCGTGGT CACCTTCACT TTCATGCACA CACCCACACA TGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Man2b1 ( XM_006255273.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255335.1 Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X4, mRNA.

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CloneID	ORa38718
Clone ID Related Accession (Same CDS sequence)	XM_006255273.3
Accession Version	XM_006255273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lysosomal alpha-mannosidase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGCAGGCAAA CGGGAGCAAG 
GTCCACGTGC TCTACTCCAC CCCTTCGTGT TACCTCTGGG AGCTGAACAA GGCCAATCTC 
ACTTGGACAG TGAAGGAGGA CGACTTCTTT CCTTATGCTG ATGGCCCCCA CATGTTCTGG 
ACTGGCTATT TCTCCAGTAG GCCAGCCCTC AAGCGCTATG AGCGCCTCAG CTACAACTTC 
CTGCAGGTGT GCAACCAGCT GGAAGCACTG GTGGGTCCCG AGGCCAAAGT GGGACCCTAC 
GGCTCAGGAG ACAGTGCACC CCTCAATGAG GCAATGGCGG TGCTGCAGCA CCACGATGCT 
GTCACTGGTA CTGCAAGACA GAATGTTGTC AATGACTATG CGAAGCAGCT GGCGGCAGGC 
TGGGGACCCT GTGAGGTTCT CGTGAGCAAC GCTCTGGCGC GACTCAGCCT TTACAAACAG 
AACTTCTCAT TCTGTCGGGA GATCAACATC AGTATCTGCC CAGCCAGCCA GACGTCAGAG 
CACTTCCGGG TAATTATTTA CAACCCCGTG GGGCGGAAGG TGGATCTGAT GGTTCGGCTG 
CCGGTCAGTG AAGGCATCTT CCTTGTGAAG GACCCTAACG ATAGGAGAAT TTCCAGTAAT 
GTGGTGATGG TTCCCAGTGC TTACAGTAAG ACGTATCAAT GGGAGCTGCT GTTCCCAGCC 
TCAGTGCCTG CCCTGGGCTT CAGCATCTAC TCTGTGAACA AAATGAGTGG CCATAACCAT 
CAGGCCCACA ACCTAACGGC CAGGCCCAAG AAATCCAAGT CCCGTGTCTT AGTCATTGAA 
AATAAGTACA TACGGGCCAC ATTTGACTCT GACACAGGGT TATTGAGGAA GATTGAAAAC 
CTGGAACAGA ACATTTCGCT CCCTGTGAGA CAAGGCTTCT TTTGGTACAA TGCCAGCGCT 
GGTGATGAGG AGAGTCCTCA GGCCTCTGGT GCCTACATCT TCAGACCCAG TCATCGTAAA 
CCACTGCCTG TGAGCCACTG GGCTCAGGTC ACTTTGGTGA AGACAAACTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTATGA AGGACAGCGT 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA AAGATGACTG GGGAAAGGAA 
GTCATCAGCC GCTTTAACAC CCCGATGAGA ACCAGGGGGC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGACGAT TTCCGACCCA CCTGGACATT AAATCAGACT 
GAACCAGTGG CTGGAAATTA CTACCCTGTC AACACTCGCA TTTACATCAC GGACGGGCAT 
ATGCAGCTGA CCGTGCTGAC CGACCGTTCC CAGGGGGGCA GCAGTCTGCT GGATGGCTCA 
TTGGAGCTCA TGGTTCACCG ACGGCTGTTG GTAGATGATG AACGTGGGGT GGCGGAACCC 
TTGCTGGAGA CAGATACGGG GGATAAGGTT CGAGGGCGCC ACCTTGTGAT TTTGAGTTCT 
GTCAGTGATG CGGCTGCAAG ACACCGACTG CTGGCGGAGC AGGAAGTCCT GGCCCCTCAG 
GTGGTGCTGG CCCACGGGGG TAGCAGTCCC TATCACTCGC AGGCACCAAA AATGCAGTTC 
TCCGCACTTC GCCGGGAGCT ACCTCCGCAG GTGCATCTGC TCACCCTGGC TCGCTGGGGT 
CCAAAGATGC TGCTGCTGCG CTTGGAGCAC CAGTTCGCTG TGAAGGAAGA TTCAAATCGC 
AACCTGAGCT CTCCGGTGAC CTTGAACTTG CAGAACCTGT TCAAGACCTT CACTATCAAC 
TACCTGCAGG AGACCACACT GGCAGCCAAC CAGCCCCTAT CCAGGGTTTC CAGGCTCAAG 
TGGATGACAG ATACTGGTCC CATCTCCTAC CCTGCTCCTT CCCGGTTGGA CCCTACCTCA 
ATCACACTGC AGCCTATGCA AATCCGTACC TTCCTGGCCT CGGTTCAATG GCAGAGACTC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255335.1
CDS	65..3130
Translation	MGTGSLAPGVRAGGGNTGWLWMSSCNLGLPVLSISFLIWLLLAAPGAQAAGYKTCPTTKPGMLNVHLLPHTHDDVGWLKTVDQYYYGMDPHLLTFMPIVMSDVQHASVQYILDSVIYSLLNDPTRRFIYVEMAFFSRWWKQQTNVTQDAVRNLVRQGRLEFVNGGWVMNDEAATHYGAIVDQMTLGLRFLQDTFGSDGLPRVAWHIDPFGHSREQASLFAQMGFDGFFLGRIDYQDKFNRKRKLKMEELWRASASLKPPAADLFTGVLPNNYNPPKDLCWDVLCTDPPVVDDPTSPEFNANKLVDYFLNLASSQKKYYRTNHTVMTMGSDFQYENANMWFKNMDKLIRLVNEQQQANGSKVHVLYSTPSCYLWELNKANLTWTVKEDDFFPYADGPHMFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGPEAKVGPYGSGDSAPLNEAMAVLQHHDAVTGTARQNVVNDYAKQLAAGWGPCEVLVSNALARLSLYKQNFSFCREINISICPASQTSEHFRVIIYNPVGRKVDLMVRLPVSEGIFLVKDPNDRRISSNVVMVPSAYSKTYQWELLFPASVPALGFSIYSVNKMSGHNHQAHNLTARPKKSKSRVLVIENKYIRATFDSDTGLLRKIENLEQNISLPVRQGFFWYNASAGDEESPQASGAYIFRPSHRKPLPVSHWAQVTLVKTNLVQEVHQNFSAWCSQVIRLYEGQRHLELEWTVGPIPVKDDWGKEVISRFNTPMRTRGQFFTDSNGREILKRRDDFRPTWTLNQTEPVAGNYYPVNTRIYITDGHMQLTVLTDRSQGGSSLLDGSLELMVHRRLLVDDERGVAEPLLETDTGDKVRGRHLVILSSVSDAAARHRLLAEQEVLAPQVVLAHGGSSPYHSQAPKMQFSALRRELPPQVHLLTLARWGPKMLLLRLEHQFAVKEDSNRNLSSPVTLNLQNLFKTFTINYLQETTLAANQPLSRVSRLKWMTDTGPISYPAPSRLDPTSITLQPMQIRTFLASVQWQRLI

Target ORF information:

RefSeq Version	XM_006255273.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mannosidase, alpha, class 2B, member 1 (Man2b1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255273.3

ORF Insert Sequence:

ATGGGCACCG GATCGCTTGC CCCTGGTGTC CGTGCTGGTG GCGGGAACAC TGGGTGGCTC 
TGGATGAGCT CCTGCAACTT AGGTTTGCCA GTCCTGTCTA TTTCTTTTCT TATTTGGTTG 
TTACTGGCTG CGCCTGGCGC TCAGGCCGCG GGTTACAAGA CATGTCCCAC CACGAAGCCA 
GGCATGCTCA ACGTGCACCT GCTGCCTCAC ACACACGATG ATGTGGGCTG GCTGAAGACG 
GTAGACCAAT ACTACTACGG CATGGACCCT CACTTGCTGA CCTTCATGCC CATAGTCATG 
AGTGACGTCC AGCATGCATC TGTTCAGTAC ATCCTAGACT CAGTGATTTA CTCTCTACTG 
AACGATCCCA CCCGTCGCTT CATATACGTG GAAATGGCTT TCTTCTCCCG TTGGTGGAAG 
CAGCAGACCA ATGTAACACA GGACGCTGTA CGGAACCTGG TGCGCCAGGG GCGCCTGGAG 
TTTGTCAATG GTGGCTGGGT AATGAATGAT GAGGCAGCCA CTCACTATGG TGCTATTGTG 
GACCAGATGA CACTTGGGCT ACGCTTCCTG CAGGACACAT TTGGTAGTGA TGGACTTCCC 
CGTGTGGCCT GGCACATTGA CCCTTTTGGC CATTCTCGAG AACAGGCCTC CCTGTTTGCC 
CAGATGGGTT TTGATGGCTT CTTCCTCGGG CGCATTGACT ACCAAGATAA ATTTAACCGG 
AAGAGAAAGC TGAAGATGGA GGAACTCTGG AGGGCTAGCG CCAGCTTGAA GCCCCCTGCT 
GCTGACCTCT TTACTGGTGT GCTCCCCAAT AATTACAACC CTCCAAAAGA CCTGTGCTGG 
GATGTGCTGT GTACAGACCC TCCCGTGGTG GACGATCCCA CAAGCCCTGA GTTTAATGCC 
AATAAGTTGG TTGATTACTT CCTGAACCTC GCCTCTTCCC AGAAAAAGTA TTACCGAACC 
AACCACACCG TGATGACCAT GGGCTCAGAC TTCCAGTATG AGAATGCCAA CATGTGGTTC 
AAAAACATGG ACAAGCTTAT CCGCCTGGTC AACGAGCAGC AGCAGGCAAA CGGGAGCAAG 
GTCCACGTGC TCTACTCCAC CCCTTCGTGT TACCTCTGGG AGCTGAACAA GGCCAATCTC 
ACTTGGACAG TGAAGGAGGA CGACTTCTTT CCTTATGCTG ATGGCCCCCA CATGTTCTGG 
ACTGGCTATT TCTCCAGTAG GCCAGCCCTC AAGCGCTATG AGCGCCTCAG CTACAACTTC 
CTGCAGGTGT GCAACCAGCT GGAAGCACTG GTGGGTCCCG AGGCCAAAGT GGGACCCTAC 
GGCTCAGGAG ACAGTGCACC CCTCAATGAG GCAATGGCGG TGCTGCAGCA CCACGATGCT 
GTCACTGGTA CTGCAAGACA GAATGTTGTC AATGACTATG CGAAGCAGCT GGCGGCAGGC 
TGGGGACCCT GTGAGGTTCT CGTGAGCAAC GCTCTGGCGC GACTCAGCCT TTACAAACAG 
AACTTCTCAT TCTGTCGGGA GATCAACATC AGTATCTGCC CAGCCAGCCA GACGTCAGAG 
CACTTCCGGG TAATTATTTA CAACCCCGTG GGGCGGAAGG TGGATCTGAT GGTTCGGCTG 
CCGGTCAGTG AAGGCATCTT CCTTGTGAAG GACCCTAACG ATAGGAGAAT TTCCAGTAAT 
GTGGTGATGG TTCCCAGTGC TTACAGTAAG ACGTATCAAT GGGAGCTGCT GTTCCCAGCC 
TCAGTGCCTG CCCTGGGCTT CAGCATCTAC TCTGTGAACA AAATGAGTGG CCATAACCAT 
CAGGCCCACA ACCTAACGGC CAGGCCCAAG AAATCCAAGT CCCGTGTCTT AGTCATTGAA 
AATAAGTACA TACGGGCCAC ATTTGACTCT GACACAGGGT TATTGAGGAA GATTGAAAAC 
CTGGAACAGA ACATTTCGCT CCCTGTGAGA CAAGGCTTCT TTTGGTACAA TGCCAGCGCT 
GGTGATGAGG AGAGTCCTCA GGCCTCTGGT GCCTACATCT TCAGACCCAG TCATCGTAAA 
CCACTGCCTG TGAGCCACTG GGCTCAGGTC ACTTTGGTGA AGACAAACTT GGTGCAGGAG 
GTACACCAGA ATTTTTCAGC CTGGTGTTCC CAAGTGATTC GCCTGTATGA AGGACAGCGT 
CATTTGGAGC TGGAGTGGAC GGTGGGACCA ATCCCAGTGA AAGATGACTG GGGAAAGGAA 
GTCATCAGCC GCTTTAACAC CCCGATGAGA ACCAGGGGGC AGTTCTTCAC GGACAGCAAC 
GGCCGGGAAA TCCTGAAGAG GAGGGACGAT TTCCGACCCA CCTGGACATT AAATCAGACT 
GAACCAGTGG CTGGAAATTA CTACCCTGTC AACACTCGCA TTTACATCAC GGACGGGCAT 
ATGCAGCTGA CCGTGCTGAC CGACCGTTCC CAGGGGGGCA GCAGTCTGCT GGATGGCTCA 
TTGGAGCTCA TGGTTCACCG ACGGCTGTTG GTAGATGATG AACGTGGGGT GGCGGAACCC 
TTGCTGGAGA CAGATACGGG GGATAAGGTT CGAGGGCGCC ACCTTGTGAT TTTGAGTTCT 
GTCAGTGATG CGGCTGCAAG ACACCGACTG CTGGCGGAGC AGGAAGTCCT GGCCCCTCAG 
GTGGTGCTGG CCCACGGGGG TAGCAGTCCC TATCACTCGC AGGCACCAAA AATGCAGTTC 
TCCGCACTTC GCCGGGAGCT ACCTCCGCAG GTGCATCTGC TCACCCTGGC TCGCTGGGGT 
CCAAAGATGC TGCTGCTGCG CTTGGAGCAC CAGTTCGCTG TGAAGGAAGA TTCAAATCGC 
AACCTGAGCT CTCCGGTGAC CTTGAACTTG CAGAACCTGT TCAAGACCTT CACTATCAAC 
TACCTGCAGG AGACCACACT GGCAGCCAAC CAGCCCCTAT CCAGGGTTTC CAGGCTCAAG 
TGGATGACAG ATACTGGTCC CATCTCCTAC CCTGCTCCTT CCCGGTTGGA CCCTACCTCA 
ATCACACTGC AGCCTATGCA AATCCGTACC TTCCTGGCCT CGGTTCAATG GCAGAGACTC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The effects of swainsonine on the activity and expression of ?-mannosidase in BRL-3A cells.
Toxicon : official journal of the International Society on Toxinology9944-50(2015 Jun)
Lu H,Ma F,Wang H,Geng PS,Wang SS,Wang JG,Wu CC,Zhao BY

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
The Journal of biological chemistry290(14)8789-802(2015 Apr)
Thaysen-Andersen M,Venkatakrishnan V,Loke I,Laurini C,Diestel S,Parker BL,Packer NH

Neurocognitive and psychotiform behavioral alterations and enhanced hippocampal long-term potentiation in transgenic mice displaying neuropathological features of human alpha-mannosidosis.
The Journal of neuroscience : the official journal of the Society for Neuroscience25(28)6539-49(2005 Jul)
D'Hooge R,L?llmann-Rauch R,Beckers T,Balschun D,Schwake M,Reiss K,von Figura K,Saftig P

Targeted disruption of the lysosomal alpha-mannosidase gene results in mice resembling a mild form of human alpha-mannosidosis.
Human molecular genetics8(8)1365-72(1999 Aug)
Stinchi S,L?llmann-Rauch R,Hartmann D,Coenen R,Beccari T,Orlacchio A,von Figura K,Saftig P

Lysosomal alpha-mannosidases of mouse tissues: characteristics of the isoenzymes, and cloning and expression of a full-length cDNA.
The Biochemical journal327 ( Pt 1)45-9(1997 Oct)
Beccari T,Appolloni MG,Costanzi E,Stinchi S,Stirling JL,Della Fazia MA,Servillo G,Viola MP,Orlacchio A

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Man2b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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